RU94023251A - Способ получения гидрофобных полипептидов, протеинов или пептидов - Google Patents

Способ получения гидрофобных полипептидов, протеинов или пептидов

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Publication number
RU94023251A
RU94023251A RU94023251/13A RU94023251A RU94023251A RU 94023251 A RU94023251 A RU 94023251A RU 94023251/13 A RU94023251/13 A RU 94023251/13A RU 94023251 A RU94023251 A RU 94023251A RU 94023251 A RU94023251 A RU 94023251A
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RU
Russia
Prior art keywords
synthesis
proteins
peptide
peptides
monomeric
Prior art date
Application number
RU94023251/13A
Other languages
English (en)
Inventor
Дебелл Хайнц
Ch]
Дрэгер Николас
Хубер Троттман Герда
Якоб Петер
Штюбер Дитрих
De]
Original Assignee
Ф.Хоффманн - Ля Рош Аг (Ch)
Ф.Хоффманн - Ля Рош АГ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ф.Хоффманн - Ля Рош Аг (Ch), Ф.Хоффманн - Ля Рош АГ filed Critical Ф.Хоффманн - Ля Рош Аг (Ch)
Publication of RU94023251A publication Critical patent/RU94023251A/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Neurology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Neurosurgery (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Hospice & Palliative Care (AREA)
  • Animal Behavior & Ethology (AREA)
  • Psychiatry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Изобретение относится к способам, предназначенным для получения и очистки гидрофобных полипептидов, протеинов или пептидов. Кроме того, в соответствии с настоящим изобретением предлагаются протеины слияния, используемые для получения и очистки вышеупомянутых гидрофобных полипептидов, протеинов или пептидов. Кроме того, в соответствии с настоящим изобретением предлагается гомогенный мономерный b-амилоидный пептид и испытания для просеивания подавляющих токсичность амилоида лекарственных препаратов, используя вышеупомянутый мономерный b-амилоидный пептид.

Claims (1)

  1. Изобретение относится к способам, предназначенным для получения и очистки гидрофобных полипептидов, протеинов или пептидов. Кроме того, в соответствии с настоящим изобретением предлагаются протеины слияния, используемые для получения и очистки вышеупомянутых гидрофобных полипептидов, протеинов или пептидов. Кроме того, в соответствии с настоящим изобретением предлагается гомогенный мономерный b-амилоидный пептид и испытания для просеивания подавляющих токсичность амилоида лекарственных препаратов, используя вышеупомянутый мономерный b-амилоидный пептид.
RU94023251/13A 1993-07-06 1994-07-04 Способ получения гидрофобных полипептидов, протеинов или пептидов RU94023251A (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93110755 1993-07-06
EP93110755.1 1993-07-06

Publications (1)

Publication Number Publication Date
RU94023251A true RU94023251A (ru) 1996-05-10

Family

ID=8213046

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94023251/13A RU94023251A (ru) 1993-07-06 1994-07-04 Способ получения гидрофобных полипептидов, протеинов или пептидов

Country Status (14)

Country Link
US (1) US5750374A (ru)
EP (2) EP0641861B1 (ru)
JP (2) JP2947401B2 (ru)
CN (3) CN1057535C (ru)
AT (1) ATE236984T1 (ru)
AU (1) AU666274B2 (ru)
CA (1) CA2125467C (ru)
DE (1) DE69432449T2 (ru)
DK (1) DK0641861T3 (ru)
ES (1) ES2194017T3 (ru)
NZ (1) NZ260907A (ru)
PT (1) PT641861E (ru)
RU (1) RU94023251A (ru)
ZA (1) ZA944686B (ru)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6303567B1 (en) 1995-03-14 2001-10-16 Praecis Pharmaceuticals, Inc . Modulators of β-amyloid peptide aggregation comprising D-amino acids
ATE218583T1 (de) 1995-03-14 2002-06-15 Praecis Pharm Inc Verbindungen mit aggregations-modulierenden wirkung auf das amyloid protein
US5817626A (en) * 1995-03-14 1998-10-06 Praecis Pharmaceuticals Incorporated Modulators of beta-amyloid peptide aggregation
US5854215A (en) * 1995-03-14 1998-12-29 Praecis Pharmaceuticals Incorporated Modulators of β-amyloid peptide aggregation
GB9618952D0 (en) 1996-09-11 1996-10-23 Sandoz Ltd Process
US5985242A (en) * 1995-10-27 1999-11-16 Praecis Pharmaceuticals, Inc. Modulators of β-amyloid peptide aggregation comprising D-amino acids
US6277826B1 (en) 1996-08-27 2001-08-21 Praecis Pharmaceuticals, Inc. Modulators of β-amyloid peptide aggregation comprising D-amino acids
WO2000052048A1 (en) 1999-03-04 2000-09-08 Praecis Pharmaceuticals Incorporated Modulators of beta-amyloid peptide aggregation comprising d-amino acids
US7569660B1 (en) 1999-06-09 2009-08-04 The University Of Chicago Recombinant prion-like proteins and materials comprising same
AU4183200A (en) * 1999-10-21 2001-04-30 Panorama Research, Inc. A general method for optimizing the expression of heterologous proteins
US7799561B2 (en) * 2002-06-12 2010-09-21 Sigma-Aldrich, Co. Affinity peptides and method for purification of recombinant proteins
FR2859221B1 (fr) * 2003-08-28 2005-10-14 Centre Nat Rech Scient Vecteur de co-expression de domaines membranaires de proteines d'enveloppe d'un virus et utilisations
US7741053B2 (en) * 2005-05-13 2010-06-22 Sigma-Aldrich Co. Processes for purification of recombinant proteins
KR100621354B1 (ko) * 2005-06-14 2006-09-08 광주과학기술원 유비퀴틴을 이용한 아밀로이드-베타 펩티드의 제조방법
WO2010063460A1 (en) * 2008-12-02 2010-06-10 Universität Zürich Recombinant production of hydrophobic peptides and fusion proteins for use in producing same
CN107001409A (zh) * 2014-09-26 2017-08-01 株式会社钟化 疏水性肽的制造方法
RU2017123283A (ru) 2014-12-01 2019-01-09 Пфенекс Инк. Партнеры по слиянию для получения пептидов
CN105001307B (zh) * 2015-07-31 2016-08-24 南京斯拜科生化实业有限公司 一种溶解难溶多肽的偶联肽链及其在液相色谱中分离纯化的应用

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EP0207044A1 (en) * 1985-06-20 1986-12-30 Monsanto Company Release of peptides from polypeptides
EP0212532A1 (en) * 1985-08-12 1987-03-04 Syntex (U.S.A.) Inc. Method for producing fusion proteins
JPS63502904A (ja) * 1986-03-24 1988-10-27 オ−ソ・フア−マシユ−チカル・コ−ポレ−シヨン 合成ｈｔｌｖ−３ペプチド、その組成物及び用途
CA1304886C (en) * 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
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CA1329124C (en) * 1987-02-02 1994-05-03 Jerald C. Sadoff Conjugate malaria vaccine
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US5416007A (en) * 1987-03-20 1995-05-16 Creative Biomolecules, Inc. Enhanced proteolytic cleavage of recombinant fusion proteins
US5124255A (en) * 1988-03-11 1992-06-23 Abbott Laboratories CKS method of protein synthesis
IT1226728B (it) * 1988-08-05 1991-02-05 Eniricerche Spa Coniugato peptidico immunologicamente attivo utile come vaccino antimalaria e metodo di immunizzazione impiegante lo stesso
US5045190A (en) * 1988-11-08 1991-09-03 Carbonell Ruben G Chromatography apparatus
DE69133372T2 (de) * 1990-06-15 2005-02-24 Scios Inc., Sunnyvale Transgenes, nicht-menschliches säugetier das die amyloidbildende pathologie der alzheimerschen krankheit zeigt
EP0546101A1 (en) * 1990-08-24 1993-06-16 The President And Fellows Of Harvard College METHOD OF INTERFERING WITH FORMATION OF $g(a)-ANTICHYMOTRYPSIN-$g(b)-PROTEIN COMPLEX AND SYNTHETIC PEPTIDES FOR USE THEREIN
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US5434050A (en) * 1991-08-13 1995-07-18 Regents Of The University Of Minnesota Labelled β-amyloid peptide and methods of screening for Alzheimer's disease

Also Published As

Publication number Publication date
ZA944686B (en) 1995-01-06
PT641861E (pt) 2003-07-31
EP0641861A3 (en) 1997-07-30
CA2125467A1 (en) 1995-01-07
EP1304381A3 (en) 2003-05-02
EP0641861B1 (en) 2003-04-09
CN100439391C (zh) 2008-12-03
CN1098416A (zh) 1995-02-08
CA2125467C (en) 2001-02-06
JP2947401B2 (ja) 1999-09-13
DK0641861T3 (da) 2003-07-28
CN1916017A (zh) 2007-02-21
AU6612294A (en) 1995-01-27
CN1227846A (zh) 1999-09-08
JPH1143500A (ja) 1999-02-16
JPH07102000A (ja) 1995-04-18
DE69432449T2 (de) 2004-04-08
EP0641861A2 (en) 1995-03-08
CN1057535C (zh) 2000-10-18
ATE236984T1 (de) 2003-04-15
NZ260907A (en) 1996-06-25
DE69432449D1 (de) 2003-05-15
US5750374A (en) 1998-05-12
ES2194017T3 (es) 2003-11-16
EP1304381A2 (en) 2003-04-23
AU666274B2 (en) 1996-02-01

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