NO331783B1 - Transkobalamin-metning testmetode samt sett for a utfore slik testing - Google Patents

Transkobalamin-metning testmetode samt sett for a utfore slik testing Download PDF

Info

Publication number: NO331783B1
Authority: NO; Norway
Prior art keywords: transcobalamin; holo; binding partner; apo; ligand
Prior art date: 2001-04-23

Application number

NO20034707A

Other languages

English (en)

Norwegian (no)

Other versions

NO20034707D0 (no

NO20034707L (no

Inventor

Lars Orning

Original Assignee

Axis Shield Asa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-04-23

Filing date

2003-10-21

Publication date

2012-03-26

2003-10-21 Application filed by Axis Shield Asa filed Critical Axis Shield Asa

2003-10-21 Publication of NO20034707D0 publication Critical patent/NO20034707D0/no

2003-12-17 Publication of NO20034707L publication Critical patent/NO20034707L/no

2012-03-26 Publication of NO331783B1 publication Critical patent/NO331783B1/no

Links

102000011409 Transcobalamins Human genes 0.000 title claims abstract description 48
108010023603 Transcobalamins Proteins 0.000 title claims abstract description 48
238000012360 testing method Methods 0.000 title claims description 28
238000010998 test method Methods 0.000 title claims description 6
238000003149 assay kit Methods 0.000 title 1
239000003446 ligand Substances 0.000 claims abstract description 55
238000009739 binding Methods 0.000 claims abstract description 53
230000027455 binding Effects 0.000 claims abstract description 51
239000000758 substrate Substances 0.000 claims abstract description 50
230000009870 specific binding Effects 0.000 claims abstract description 38
239000007788 liquid Substances 0.000 claims abstract description 12
230000003100 immobilizing effect Effects 0.000 claims abstract description 11
238000000034 method Methods 0.000 claims description 45
238000002198 surface plasmon resonance spectroscopy Methods 0.000 claims description 16
238000001514 detection method Methods 0.000 claims description 11
238000002372 labelling Methods 0.000 claims description 7
230000002255 enzymatic effect Effects 0.000 claims description 6
239000001913 cellulose Substances 0.000 claims description 5
229920002678 cellulose Polymers 0.000 claims description 5
210000004369 blood Anatomy 0.000 claims description 4
239000008280 blood Substances 0.000 claims description 4
239000003599 detergent Substances 0.000 claims description 3
230000005670 electromagnetic radiation Effects 0.000 claims description 2
239000011358 absorbing material Substances 0.000 claims 1
238000003556 assay Methods 0.000 abstract 1
FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 44
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 17
239000011324 bead Substances 0.000 description 16
108020004414 DNA Proteins 0.000 description 11
208000002670 vitamin B12 deficiency Diseases 0.000 description 10
108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
102000004169 proteins and genes Human genes 0.000 description 9
108090000623 proteins and genes Proteins 0.000 description 9
210000002966 serum Anatomy 0.000 description 9
LMDZBCPBFSXMTL-UHFFFAOYSA-N 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide Chemical compound CCN=C=NCCCN(C)C LMDZBCPBFSXMTL-UHFFFAOYSA-N 0.000 description 8
238000012216 screening Methods 0.000 description 8
239000011780 sodium chloride Substances 0.000 description 8
102000004190 Enzymes Human genes 0.000 description 7
108090000790 Enzymes Proteins 0.000 description 7
239000011230 binding agent Substances 0.000 description 7
150000001875 compounds Chemical class 0.000 description 7
239000000203 mixture Substances 0.000 description 7
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
101100075829 Caenorhabditis elegans mab-3 gene Proteins 0.000 description 6
102000053602 DNA Human genes 0.000 description 6
239000007983 Tris buffer Substances 0.000 description 6
210000004027 cell Anatomy 0.000 description 6
238000006243 chemical reaction Methods 0.000 description 6
239000010410 layer Substances 0.000 description 6
210000004379 membrane Anatomy 0.000 description 6
239000012528 membrane Substances 0.000 description 6
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
101100476210 Caenorhabditis elegans rnt-1 gene Proteins 0.000 description 5
102000008394 Immunoglobulin Fragments Human genes 0.000 description 5
108010021625 Immunoglobulin Fragments Proteins 0.000 description 5
239000012148 binding buffer Substances 0.000 description 5
238000000338 in vitro Methods 0.000 description 5
239000000243 solution Substances 0.000 description 5
239000000126 substance Substances 0.000 description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 4
102000002260 Alkaline Phosphatase Human genes 0.000 description 4
108020004774 Alkaline Phosphatase Proteins 0.000 description 4
229920002307 Dextran Polymers 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
108020004682 Single-Stranded DNA Proteins 0.000 description 4
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
210000001124 body fluid Anatomy 0.000 description 4
239000010839 body fluid Substances 0.000 description 4
230000008878 coupling Effects 0.000 description 4
238000010168 coupling process Methods 0.000 description 4
238000005859 coupling reaction Methods 0.000 description 4
239000008367 deionised water Substances 0.000 description 4
229910021641 deionized water Inorganic materials 0.000 description 4
239000000463 material Substances 0.000 description 4
238000005259 measurement Methods 0.000 description 4
238000002823 phage display Methods 0.000 description 4
239000000047 product Substances 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
239000003656 tris buffered saline Substances 0.000 description 4
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 description 3
108091034117 Oligonucleotide Proteins 0.000 description 3
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
102100040423 Transcobalamin-2 Human genes 0.000 description 3
101710124862 Transcobalamin-2 Proteins 0.000 description 3
239000002250 absorbent Substances 0.000 description 3
230000002745 absorbent Effects 0.000 description 3
239000006096 absorbing agent Substances 0.000 description 3
239000003463 adsorbent Substances 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
238000011161 development Methods 0.000 description 3
230000018109 developmental process Effects 0.000 description 3
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
235000013305 food Nutrition 0.000 description 3
210000003405 ileum Anatomy 0.000 description 3
229940029329 intrinsic factor Drugs 0.000 description 3
239000002609 medium Substances 0.000 description 3
244000005700 microbiome Species 0.000 description 3
239000002245 particle Substances 0.000 description 3
239000002953 phosphate buffered saline Substances 0.000 description 3
229920001223 polyethylene glycol Polymers 0.000 description 3
229920000136 polysorbate Polymers 0.000 description 3
230000008569 process Effects 0.000 description 3
108090000765 processed proteins & peptides Proteins 0.000 description 3
102000005962 receptors Human genes 0.000 description 3
125000006853 reporter group Chemical group 0.000 description 3
238000000926 separation method Methods 0.000 description 3
-1 sheets Substances 0.000 description 3
239000011782 vitamin Substances 0.000 description 3
235000013343 vitamin Nutrition 0.000 description 3
229940088594 vitamin Drugs 0.000 description 3
229930003231 vitamin Natural products 0.000 description 3
150000003722 vitamin derivatives Chemical class 0.000 description 3
NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
108010088751 Albumins Proteins 0.000 description 2
102000009027 Albumins Human genes 0.000 description 2
108091023037 Aptamer Proteins 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
PCDQPRRSZKQHHS-XVFCMESISA-N CTP Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-XVFCMESISA-N 0.000 description 2
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 2
241000287828 Gallus gallus Species 0.000 description 2
108091006905 Human Serum Albumin Proteins 0.000 description 2
102000008100 Human Serum Albumin Human genes 0.000 description 2
MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
241000124008 Mammalia Species 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 2
206010060860 Neurological symptom Diseases 0.000 description 2
238000012408 PCR amplification Methods 0.000 description 2
239000002202 Polyethylene glycol Substances 0.000 description 2
229920001213 Polysorbate 20 Polymers 0.000 description 2
206010037180 Psychiatric symptoms Diseases 0.000 description 2
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
PGAVKCOVUIYSFO-XVFCMESISA-N UTP Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-XVFCMESISA-N 0.000 description 2
238000010521 absorption reaction Methods 0.000 description 2
150000001408 amides Chemical class 0.000 description 2
239000012491 analyte Substances 0.000 description 2
208000007502 anemia Diseases 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
239000000872 buffer Substances 0.000 description 2
210000000170 cell membrane Anatomy 0.000 description 2
235000013330 chicken meat Nutrition 0.000 description 2
239000005515 coenzyme Substances 0.000 description 2
239000002299 complementary DNA Substances 0.000 description 2
230000009260 cross reactivity Effects 0.000 description 2
230000007812 deficiency Effects 0.000 description 2
230000002950 deficient Effects 0.000 description 2
239000000412 dendrimer Substances 0.000 description 2
229920000736 dendritic polymer Polymers 0.000 description 2
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
239000000975 dye Substances 0.000 description 2
239000012530 fluid Substances 0.000 description 2
230000004927 fusion Effects 0.000 description 2
239000000499 gel Substances 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
210000004408 hybridoma Anatomy 0.000 description 2
FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
238000003018 immunoassay Methods 0.000 description 2
210000004185 liver Anatomy 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
230000011987 methylation Effects 0.000 description 2
238000007069 methylation reaction Methods 0.000 description 2
ZIYVHBGGAOATLY-UHFFFAOYSA-N methylmalonic acid Chemical compound OC(=O)C(C)C(O)=O ZIYVHBGGAOATLY-UHFFFAOYSA-N 0.000 description 2
229940126619 mouse monoclonal antibody Drugs 0.000 description 2
239000002773 nucleotide Substances 0.000 description 2
125000003729 nucleotide group Chemical group 0.000 description 2
239000008363 phosphate buffer Substances 0.000 description 2
230000036470 plasma concentration Effects 0.000 description 2
239000013612 plasmid Substances 0.000 description 2
229920003023 plastic Polymers 0.000 description 2
239000004033 plastic Substances 0.000 description 2
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
229920001184 polypeptide Polymers 0.000 description 2
102000004196 processed proteins & peptides Human genes 0.000 description 2
238000011002 quantification Methods 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
239000003488 releasing hormone Substances 0.000 description 2
238000012163 sequencing technique Methods 0.000 description 2
235000017557 sodium bicarbonate Nutrition 0.000 description 2
229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
239000007790 solid phase Substances 0.000 description 2
238000001179 sorption measurement Methods 0.000 description 2
ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
238000010561 standard procedure Methods 0.000 description 2
210000001519 tissue Anatomy 0.000 description 2
238000013518 transcription Methods 0.000 description 2
230000035897 transcription Effects 0.000 description 2
PGAVKCOVUIYSFO-UHFFFAOYSA-N uridine-triphosphate Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 PGAVKCOVUIYSFO-UHFFFAOYSA-N 0.000 description 2
MZFOKIKEPGUZEN-AGCMQPJKSA-N (R)-methylmalonyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)[C@@H](C(O)=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MZFOKIKEPGUZEN-AGCMQPJKSA-N 0.000 description 1
JNGRENQDBKMCCR-UHFFFAOYSA-N 2-(3-amino-6-iminoxanthen-9-yl)benzoic acid;hydrochloride Chemical compound [Cl-].C=12C=CC(=[NH2+])C=C2OC2=CC(N)=CC=C2C=1C1=CC=CC=C1C(O)=O JNGRENQDBKMCCR-UHFFFAOYSA-N 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
OALHHIHQOFIMEF-UHFFFAOYSA-N 3',6'-dihydroxy-2',4',5',7'-tetraiodo-3h-spiro[2-benzofuran-1,9'-xanthene]-3-one Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 OALHHIHQOFIMEF-UHFFFAOYSA-N 0.000 description 1
FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 1
229940122720 Alkaline phosphatase inhibitor Drugs 0.000 description 1
208000004300 Atrophic Gastritis Diseases 0.000 description 1
208000023275 Autoimmune disease Diseases 0.000 description 1
241000713838 Avian myeloblastosis virus Species 0.000 description 1
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
102000004506 Blood Proteins Human genes 0.000 description 1
108010017384 Blood Proteins Proteins 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
108010001857 Cell Surface Receptors Proteins 0.000 description 1
PCDQPRRSZKQHHS-UHFFFAOYSA-N Cytidine 5'-triphosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 PCDQPRRSZKQHHS-UHFFFAOYSA-N 0.000 description 1
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical compound C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 description 1
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 1
BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
208000005577 Gastroenteritis Diseases 0.000 description 1
208000018522 Gastrointestinal disease Diseases 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
235000000177 Indigofera tinctoria Nutrition 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
108060001084 Luciferase Proteins 0.000 description 1
239000005089 Luciferase Substances 0.000 description 1
DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
239000007993 MOPS buffer Substances 0.000 description 1
241000699670 Mus sp. Species 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
108091028043 Nucleic acid sequence Proteins 0.000 description 1
102000015636 Oligopeptides Human genes 0.000 description 1
108010038807 Oligopeptides Proteins 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
241001494479 Pecora Species 0.000 description 1
208000031845 Pernicious anaemia Diseases 0.000 description 1
208000007452 Plasmacytoma Diseases 0.000 description 1
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 1
108091008103 RNA aptamers Proteins 0.000 description 1
108091028664 Ribonucleotide Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
101710137500 T7 RNA polymerase Proteins 0.000 description 1
239000013504 Triton X-100 Substances 0.000 description 1
229920004890 Triton X-100 Polymers 0.000 description 1
229930003779 Vitamin B12 Natural products 0.000 description 1
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
238000009825 accumulation Methods 0.000 description 1
150000001251 acridines Chemical class 0.000 description 1
230000009471 action Effects 0.000 description 1
230000001154 acute effect Effects 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
229960003896 aminopterin Drugs 0.000 description 1
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
229910052921 ammonium sulfate Inorganic materials 0.000 description 1
239000001166 ammonium sulphate Substances 0.000 description 1
235000011130 ammonium sulphate Nutrition 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
125000000129 anionic group Chemical group 0.000 description 1
150000004056 anthraquinones Chemical class 0.000 description 1
239000002518 antifoaming agent Substances 0.000 description 1
JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical compound N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 description 1
239000000987 azo dye Substances 0.000 description 1
230000008901 benefit Effects 0.000 description 1
ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
102000005936 beta-Galactosidase Human genes 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
238000005415 bioluminescence Methods 0.000 description 1
230000029918 bioluminescence Effects 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
230000003139 buffering effect Effects 0.000 description 1
235000021466 carotenoid Nutrition 0.000 description 1
150000001747 carotenoids Chemical class 0.000 description 1
210000001175 cerebrospinal fluid Anatomy 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
229930002875 chlorophyll Natural products 0.000 description 1
235000019804 chlorophyll Nutrition 0.000 description 1
239000001752 chlorophylls and chlorophyllins Substances 0.000 description 1
208000016644 chronic atrophic gastritis Diseases 0.000 description 1
230000001684 chronic effect Effects 0.000 description 1
239000007979 citrate buffer Substances 0.000 description 1
238000010367 cloning Methods 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
229940010007 cobalamins Drugs 0.000 description 1
150000001867 cobalamins Chemical class 0.000 description 1
GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical group [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
WUPRCGRRQUZFAB-DEGKJRJSSA-N corrin Chemical group N1C2CC\C1=C\C(CC/1)=N\C\1=C/C(CC\1)=N/C/1=C\C1=NC2CC1 WUPRCGRRQUZFAB-DEGKJRJSSA-N 0.000 description 1
235000001671 coumarin Nutrition 0.000 description 1
150000004775 coumarins Chemical class 0.000 description 1
239000012228 culture supernatant Substances 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
239000005547 deoxyribonucleotide Substances 0.000 description 1
125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
238000002405 diagnostic procedure Methods 0.000 description 1
230000001079 digestive effect Effects 0.000 description 1
238000007865 diluting Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
239000000539 dimer Substances 0.000 description 1
238000013399 early diagnosis Methods 0.000 description 1
230000000694 effects Effects 0.000 description 1
238000004520 electroporation Methods 0.000 description 1
238000010828 elution Methods 0.000 description 1
210000003989 endothelium vascular Anatomy 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
210000001842 enterocyte Anatomy 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000000835 fiber Substances 0.000 description 1
210000002950 fibroblast Anatomy 0.000 description 1
229920002457 flexible plastic Polymers 0.000 description 1
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
239000012634 fragment Substances 0.000 description 1
238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
210000001156 gastric mucosa Anatomy 0.000 description 1
210000001035 gastrointestinal tract Anatomy 0.000 description 1
239000008273 gelatin Substances 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000007973 glycine-HCl buffer Substances 0.000 description 1
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
239000010931 gold Substances 0.000 description 1
229910052737 gold Inorganic materials 0.000 description 1
230000011132 hemopoiesis Effects 0.000 description 1
230000003053 immunization Effects 0.000 description 1
238000002649 immunization Methods 0.000 description 1
230000001771 impaired effect Effects 0.000 description 1
230000006872 improvement Effects 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
229940097275 indigo Drugs 0.000 description 1
COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
230000002427 irreversible effect Effects 0.000 description 1
238000004020 luminiscence type Methods 0.000 description 1
HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
210000002540 macrophage Anatomy 0.000 description 1
102000006240 membrane receptors Human genes 0.000 description 1
230000007102 metabolic function Effects 0.000 description 1
239000002207 metabolite Substances 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
230000002906 microbiologic effect Effects 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
210000000653 nervous system Anatomy 0.000 description 1
230000000926 neurological effect Effects 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
150000003901 oxalic acid esters Chemical class 0.000 description 1
150000004893 oxazines Chemical class 0.000 description 1
238000004091 panning Methods 0.000 description 1
239000008188 pellet Substances 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
108060006184 phycobiliprotein Proteins 0.000 description 1
239000000049 pigment Substances 0.000 description 1
230000008884 pinocytosis Effects 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
229950008882 polysorbate Drugs 0.000 description 1
150000004032 porphyrins Chemical class 0.000 description 1
239000000843 powder Substances 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
238000004393 prognosis Methods 0.000 description 1
125000000168 pyrrolyl group Chemical group 0.000 description 1
230000005855 radiation Effects 0.000 description 1
239000000700 radioactive tracer Substances 0.000 description 1
230000008707 rearrangement Effects 0.000 description 1
230000009467 reduction Effects 0.000 description 1
238000004153 renaturation Methods 0.000 description 1
230000004044 response Effects 0.000 description 1
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
229940043267 rhodamine b Drugs 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
210000003296 saliva Anatomy 0.000 description 1
210000000582 semen Anatomy 0.000 description 1
230000011664 signaling Effects 0.000 description 1
229910021487 silica fume Inorganic materials 0.000 description 1
239000000741 silica gel Substances 0.000 description 1
229910002027 silica gel Inorganic materials 0.000 description 1
239000000377 silicon dioxide Substances 0.000 description 1
239000002356 single layer Substances 0.000 description 1
235000020183 skimmed milk Nutrition 0.000 description 1
239000001632 sodium acetate Substances 0.000 description 1
235000017281 sodium acetate Nutrition 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
239000007787 solid Substances 0.000 description 1
229940063673 spermidine Drugs 0.000 description 1
210000000952 spleen Anatomy 0.000 description 1
210000004988 splenocyte Anatomy 0.000 description 1
210000002784 stomach Anatomy 0.000 description 1
230000004206 stomach function Effects 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
VNOYUJKHFWYWIR-ITIYDSSPSA-N succinyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCC(O)=O)O[C@H]1N1C2=NC=NC(N)=C2N=C1 VNOYUJKHFWYWIR-ITIYDSSPSA-N 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
150000004897 thiazines Chemical class 0.000 description 1
RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
229940033663 thimerosal Drugs 0.000 description 1
150000003573 thiols Chemical class 0.000 description 1
150000005075 thioxanthenes Chemical class 0.000 description 1
229940104230 thymidine Drugs 0.000 description 1
238000012546 transfer Methods 0.000 description 1
230000032258 transport Effects 0.000 description 1
150000003918 triazines Chemical class 0.000 description 1
239000013638 trimer Substances 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
241001148471 unidentified anaerobic bacterium Species 0.000 description 1
210000002438 upper gastrointestinal tract Anatomy 0.000 description 1
235000019156 vitamin B Nutrition 0.000 description 1
239000011720 vitamin B Substances 0.000 description 1
239000011715 vitamin B12 Substances 0.000 description 1
235000019163 vitamin B12 Nutrition 0.000 description 1
229940046001 vitamin b complex Drugs 0.000 description 1
238000005406 washing Methods 0.000 description 1
125000001834 xanthenyl group Chemical class C1=CC=CC=2OC3=CC=CC=C3C(C12)* 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
- G01N33/5735—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes co-enzymes or co-factors, e.g. NAD, ATP
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/32—Immunoglobulins specific features characterized by aspects of specificity or valency specific for a neo-epitope on a complex, e.g. antibody-antigen or ligand-receptor

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Organic Chemistry (AREA)
Physics & Mathematics (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Microbiology (AREA)
Cell Biology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Food Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Genetics & Genomics (AREA)
Biophysics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Investigating Or Analysing Biological Materials (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Semiconductor Lasers (AREA)

NO20034707A 2001-04-23 2003-10-21 Transkobalamin-metning testmetode samt sett for a utfore slik testing NO331783B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB0109925A GB0109925D0 (en)	2001-04-23	2001-04-23	Method
PCT/GB2002/001846 WO2002086513A2 (en)	2001-04-23	2002-04-23	Transcobalamin ii assay method

Publications (3)

Publication Number	Publication Date
NO20034707D0 NO20034707D0 (no)	2003-10-21
NO20034707L NO20034707L (no)	2003-12-17
NO331783B1 true NO331783B1 (no)	2012-03-26

Family

ID=9913282

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO20034707A NO331783B1 (no)	2001-04-23	2003-10-21	Transkobalamin-metning testmetode samt sett for a utfore slik testing

Country Status (13)

Country	Link
US (2)	US7501287B2 (ja)
EP (1)	EP1384083B1 (ja)
JP (1)	JP3935437B2 (ja)
AT (1)	ATE285582T1 (ja)
AU (1)	AU2002255117B2 (ja)
CA (1)	CA2445089A1 (ja)
DE (1)	DE60202361T2 (ja)
ES (1)	ES2235034T3 (ja)
GB (1)	GB0109925D0 (ja)
NO (1)	NO331783B1 (ja)
PT (1)	PT1384083E (ja)
TR (1)	TR200402398T3 (ja)
WO (1)	WO2002086513A2 (ja)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE60332479D1 (de) *	2002-11-26	2010-06-17	Axis Shield Asa	Holo-transcobalamin bindungspartner und ihre verwendung in cobalamintest
GB0305904D0 (en) *	2003-03-14	2003-04-23	Cobento Biotech Aps	Test
US8916389B2 (en)	2006-06-26	2014-12-23	Nippon Telegraph And Telephone Corporation	Sensor element for SPR measurement
JP5553615B2 (ja) *	2007-03-01	2014-07-16	アボット・ラボラトリーズ	プロゾーン現象の低減を示すイムノアッセイ
US8835120B2 (en) *	2009-12-02	2014-09-16	Abbott Laboratories	Assay for cardiac troponin-T (cTnT)
EP2831582A4 (en) *	2012-03-31	2015-12-23	Nvigen Inc	MULTIFUNCTIONAL NANOPARTICLES FOR SEPARATION, DETECTION AND QUANTIFICATION OF MOLECULES AND CELLS
US9157910B2 (en)	2013-03-15	2015-10-13	Abbott Laboratories	Assay with increased dynamic range
WO2021030166A1 (en) *	2019-08-09	2021-02-18	Siemens Healthcare Diagnostics Inc.	Anti-hog tcn1 monoclonal antibodies and methods of production and use thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4273757A (en) *	1977-06-02	1981-06-16	Yissum Research Development Company	Determination of transcobalamins
US4595661A (en) *	1983-11-18	1986-06-17	Beckman Instruments, Inc.	Immunoassays and kits for use therein which include low affinity antibodies for reducing the hook effect
US4632901A (en) *	1984-05-11	1986-12-30	Hybritech Incorporated	Method and apparatus for immunoassays
GB8501671D0 (en) *	1985-01-23	1985-02-27	Allen G J	Immunoassay
FI884505A (fi) *	1987-10-02	1989-04-03	Du Pont	Immunoanalys, i vilken anvaends igg-uppsnappande antikroppar och detekteringssystem foer flera monoklonala antikroppar.
US5279937A (en) *	1992-04-30	1994-01-18	Detechnology Canada	Use of macroglobulins to improve the signal-to-background ratio in affinity binding assays
ATE163679T1 (de) *	1992-05-08	1998-03-15	Receptagen Corp	Anti-rezeptor antikörper gegen den vitamin b12/transcobalamin ii rezeptor
US5485277A (en) *	1994-07-26	1996-01-16	Physical Optics Corporation	Surface plasmon resonance sensor and methods for the utilization thereof
DE69633780T3 (de) *	1995-04-18	2011-05-05	Biosite Incorporated, San Diego	Verfahren zum assay von troponin i und t und komplexen von troponin i und t und auswahl von antikörpern zur verwendung in immunoassays
DE19629657A1 (de) *	1996-07-23	1998-01-29	Boehringer Mannheim Gmbh	Volumenunabhängiger diagnostischer Testträger und Verfahren zur Bestimmung von Analyt mit dessen Hilfe
JP3334558B2 (ja) *	1997-04-23	2002-10-15	富士レビオ株式会社	酵素免疫測定方法及び試験片
GB9820473D0 (en)	1998-09-18	1998-11-11	Axis Biochemicals Asa	Assay
KR100348351B1 (ko) *	2000-05-24	2002-08-09	주식회사 바이오디지트	전기화학 멤브레인 스트립 바이오센서

2001
- 2001-04-23 GB GB0109925A patent/GB0109925D0/en not_active Ceased
2002
- 2002-04-23 PT PT02724424T patent/PT1384083E/pt unknown
- 2002-04-23 JP JP2002583990A patent/JP3935437B2/ja not_active Expired - Fee Related
- 2002-04-23 CA CA 2445089 patent/CA2445089A1/en not_active Abandoned
- 2002-04-23 TR TR200402398T patent/TR200402398T3/xx unknown
- 2002-04-23 EP EP02724424A patent/EP1384083B1/en not_active Expired - Lifetime
- 2002-04-23 US US10/474,506 patent/US7501287B2/en not_active Expired - Lifetime
- 2002-04-23 WO PCT/GB2002/001846 patent/WO2002086513A2/en active IP Right Grant
- 2002-04-23 AT AT02724424T patent/ATE285582T1/de not_active IP Right Cessation
- 2002-04-23 AU AU2002255117A patent/AU2002255117B2/en not_active Ceased
- 2002-04-23 DE DE2002602361 patent/DE60202361T2/de not_active Expired - Lifetime
- 2002-04-23 ES ES02724424T patent/ES2235034T3/es not_active Expired - Lifetime
2003
- 2003-10-21 NO NO20034707A patent/NO331783B1/no not_active IP Right Cessation
2008
- 2008-10-24 US US12/289,291 patent/US9140710B2/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
ATE285582T1 (de)	2005-01-15
JP2004526973A (ja)	2004-09-02
US20100099129A1 (en)	2010-04-22
TR200402398T3 (tr)	2004-11-22
US9140710B2 (en)	2015-09-22
NO20034707D0 (no)	2003-10-21
DE60202361D1 (de)	2005-01-27
CA2445089A1 (en)	2002-10-31
WO2002086513A2 (en)	2002-10-31
WO2002086513A3 (en)	2003-04-10
JP3935437B2 (ja)	2007-06-20
EP1384083B1 (en)	2004-12-22
AU2002255117B2 (en)	2008-04-03
DE60202361T2 (de)	2005-12-22
NO20034707L (no)	2003-12-17
ES2235034T3 (es)	2005-07-01
GB0109925D0 (en)	2001-06-13
PT1384083E (pt)	2005-04-29
US20040132213A1 (en)	2004-07-08
US7501287B2 (en)	2009-03-10
EP1384083A2 (en)	2004-01-28

Legal Events

Date	Code	Title	Description
2022-05-09	MK1K	Patent expired

Publication	Publication Date	Title
US9140710B2 (en)	2015-09-22	Transcobalamin II assay method
US8802382B2 (en)	2014-08-12	Cobalamin assay
JP2018525637A (ja)	2018-09-06	Ｐｅｇ化分析物特異的結合剤を用いた粒子に基づくイムノアッセイ
AU2002255117A1 (en)	2003-04-17	Transcobalamin II assay method
JP2022528465A (ja)	2022-06-10	対称性ジメチル化アルギニン分析物に対する抗体及びその用途
US7279283B1 (en)	2007-10-09	Cobalamin assay
EP1030179B1 (en)	2005-11-09	Method for assaying human thymidylate synthase and assay kit
JP4663831B2 (ja)	2011-04-06	モノクローナル抗体、細胞株及びｎ１，ｎ１２−ジアセチルスペルミンの測定法
PT1257830E (pt)	2007-06-08	Ensaio para medição da holo-transcobalamina e do folato
JP2000146981A (ja)	2000-05-26	ヒトチミジル酸シンタ―ゼの測定方法及び測定用キット
JP2020186175A (ja)	2020-11-19	免疫グロブリンａに結合しているペリオスチン並びに免疫グロブリンａに結合しているペリオスチンに結合する抗体、ペリオスチンの測定方法、ペリオスチンの測定試薬及びペリオスチン測定の正確性の改善方法
JPH0798316A (ja)	1995-04-11	酵素免疫測定法
NZ525253A (en)	2004-08-27	Cobalamin assay
JPH0682456A (ja)	1994-03-22	ヒトｎｏｓに結合する抗体およびヒトｎｏｓの免疫測定法