NO315323B1 - Isolering av nukleinsyrer - Google Patents

Isolering av nukleinsyrer Download PDF

Info

Publication number: NO315323B1
Authority: NO; Norway
Prior art keywords: solid phase; nucleic acid; nucleic acids; value; blood sample
Prior art date: 1997-12-06

Application number

NO20002540A

Other languages

English (en)

Norwegian (no)

Other versions

NO20002540D0 (no

NO20002540L (no

Inventor

Matthew John Baker

Original Assignee

Dna Res Innovations Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1997-12-06

Filing date

2000-05-16

Publication date

2003-08-18

Family has litigation

First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26312726&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NO315323(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.

1997-12-06 Priority claimed from GBGB9725839.6A external-priority patent/GB9725839D0/en

1998-07-17 Priority claimed from GBGB9815541.9A external-priority patent/GB9815541D0/en

2000-05-16 Application filed by Dna Res Innovations Ltd filed Critical Dna Res Innovations Ltd

2000-05-16 Publication of NO20002540D0 publication Critical patent/NO20002540D0/no

2000-07-07 Publication of NO20002540L publication Critical patent/NO20002540L/no

2003-08-18 Publication of NO315323B1 publication Critical patent/NO315323B1/no

Links

108020004707 nucleic acids Proteins 0.000 title claims abstract description 47
102000039446 nucleic acids Human genes 0.000 title claims abstract description 47
150000007523 nucleic acids Chemical class 0.000 title claims abstract description 47
238000002955 isolation Methods 0.000 title description 7
238000000034 method Methods 0.000 claims abstract description 43
239000007790 solid phase Substances 0.000 claims abstract description 35
210000004369 blood Anatomy 0.000 claims abstract description 34
239000008280 blood Substances 0.000 claims abstract description 34
239000011521 glass Substances 0.000 claims abstract description 9
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims abstract description 3
239000004033 plastic Substances 0.000 claims description 16
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 12
239000000463 material Substances 0.000 claims description 11
239000012620 biological material Substances 0.000 claims description 9
238000003752 polymerase chain reaction Methods 0.000 claims description 9
238000004458 analytical method Methods 0.000 claims description 7
239000000872 buffer Substances 0.000 claims description 7
238000000605 extraction Methods 0.000 claims description 7
229920002704 polyhistidine Polymers 0.000 claims description 7
-1 polypropylene Polymers 0.000 claims description 7
238000002156 mixing Methods 0.000 claims description 6
239000004743 Polypropylene Substances 0.000 claims description 5
229920001155 polypropylene Polymers 0.000 claims description 5
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 4
239000003795 chemical substances by application Substances 0.000 claims description 4
239000000356 contaminant Substances 0.000 claims description 4
125000000524 functional group Chemical group 0.000 claims description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
108091005804 Peptidases Proteins 0.000 claims description 3
239000004365 Protease Substances 0.000 claims description 3
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
230000003196 chaotropic effect Effects 0.000 claims description 3
239000003456 ion exchange resin Substances 0.000 claims description 3
229920003303 ion-exchange polymer Polymers 0.000 claims description 3
150000002500 ions Chemical class 0.000 claims description 3
230000007935 neutral effect Effects 0.000 claims description 3
150000003839 salts Chemical class 0.000 claims description 3
239000011343 solid material Substances 0.000 claims description 3
239000004793 Polystyrene Substances 0.000 claims description 2
229910010293 ceramic material Inorganic materials 0.000 claims description 2
150000001875 compounds Chemical class 0.000 claims description 2
230000008878 coupling Effects 0.000 claims description 2
238000010168 coupling process Methods 0.000 claims description 2
238000005859 coupling reaction Methods 0.000 claims description 2
239000003599 detergent Substances 0.000 claims description 2
239000003085 diluting agent Substances 0.000 claims description 2
238000012252 genetic analysis Methods 0.000 claims description 2
238000010353 genetic engineering Methods 0.000 claims description 2
150000004676 glycans Chemical class 0.000 claims description 2
239000000815 hypotonic solution Substances 0.000 claims description 2
125000002883 imidazolyl group Chemical group 0.000 claims description 2
230000003993 interaction Effects 0.000 claims description 2
239000002773 nucleotide Substances 0.000 claims description 2
125000003729 nucleotide group Chemical group 0.000 claims description 2
239000007800 oxidant agent Substances 0.000 claims description 2
230000001590 oxidative effect Effects 0.000 claims description 2
229920000768 polyamine Polymers 0.000 claims description 2
229920001282 polysaccharide Polymers 0.000 claims description 2
239000005017 polysaccharide Substances 0.000 claims description 2
229920002223 polystyrene Polymers 0.000 claims description 2
239000011148 porous material Substances 0.000 claims description 2
238000000746 purification Methods 0.000 claims description 2
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims 2
238000005349 anion exchange Methods 0.000 claims 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
239000002907 paramagnetic material Substances 0.000 claims 1
238000003756 stirring Methods 0.000 claims 1
239000011324 bead Substances 0.000 abstract description 17
239000011230 binding agent Substances 0.000 abstract description 4
230000002934 lysing effect Effects 0.000 abstract description 3
210000000601 blood cell Anatomy 0.000 abstract 1
108020004414 DNA Proteins 0.000 description 19
229920001213 Polysorbate 20 Polymers 0.000 description 6
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 6
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 6
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
210000004027 cell Anatomy 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
230000005298 paramagnetic effect Effects 0.000 description 3
239000000243 solution Substances 0.000 description 3
239000002904 solvent Substances 0.000 description 3
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
238000007400 DNA extraction Methods 0.000 description 2
102000035195 Peptidases Human genes 0.000 description 2
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
RJURFGZVJUQBHK-UHFFFAOYSA-N actinomycin D Natural products CC1OC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)C2CCCN2C(=O)C(C(C)C)NC(=O)C1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)NC4C(=O)NC(C(N5CCCC5C(=O)N(C)CC(=O)N(C)C(C(C)C)C(=O)OC4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-UHFFFAOYSA-N 0.000 description 2
239000003153 chemical reaction reagent Substances 0.000 description 2
239000003480 eluent Substances 0.000 description 2
238000009830 intercalation Methods 0.000 description 2
230000005291 magnetic effect Effects 0.000 description 2
239000011347 resin Substances 0.000 description 2
229920005989 resin Polymers 0.000 description 2
239000000377 silicon dioxide Substances 0.000 description 2
239000000126 substance Substances 0.000 description 2
229920000936 Agarose Polymers 0.000 description 1
108010092160 Dactinomycin Proteins 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
108020005120 Plant DNA Proteins 0.000 description 1
RJURFGZVJUQBHK-IIXSONLDSA-N actinomycin D Chemical compound C[C@H]1OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]2CCCN2C(=O)[C@@H](C(C)C)NC(=O)[C@H]1NC(=O)C1=C(N)C(=O)C(C)=C2OC(C(C)=CC=C3C(=O)N[C@@H]4C(=O)N[C@@H](C(N5CCC[C@H]5C(=O)N(C)CC(=O)N(C)[C@@H](C(C)C)C(=O)O[C@@H]4C)=O)C(C)C)=C3N=C21 RJURFGZVJUQBHK-IIXSONLDSA-N 0.000 description 1
125000000129 anionic group Chemical group 0.000 description 1
150000001491 aromatic compounds Chemical class 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
210000002421 cell wall Anatomy 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
238000012412 chemical coupling Methods 0.000 description 1
125000003636 chemical group Chemical group 0.000 description 1
239000007822 coupling agent Substances 0.000 description 1
229960000640 dactinomycin Drugs 0.000 description 1
230000029087 digestion Effects 0.000 description 1
239000000975 dye Substances 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
210000003608 fece Anatomy 0.000 description 1
230000005660 hydrophilic surface Effects 0.000 description 1
230000005661 hydrophobic surface Effects 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
238000011534 incubation Methods 0.000 description 1
239000004413 injection moulding compound Substances 0.000 description 1
230000002687 intercalation Effects 0.000 description 1
238000004255 ion exchange chromatography Methods 0.000 description 1
239000003446 ligand Substances 0.000 description 1
239000000203 mixture Substances 0.000 description 1
239000002991 molded plastic Substances 0.000 description 1
NHNBFGGVMKEFGY-UHFFFAOYSA-N nitrate group Chemical group [N+](=O)([O-])[O-] NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
239000002245 particle Substances 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
229920000053 polysorbate 80 Polymers 0.000 description 1
239000012286 potassium permanganate Substances 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
238000005185 salting out Methods 0.000 description 1
238000005070 sampling Methods 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000002689 soil Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
238000001228 spectrum Methods 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
238000005406 washing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biomedical Technology (AREA)
Genetics & Genomics (AREA)
Biotechnology (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Analytical Chemistry (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Crystallography & Structural Chemistry (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Investigating Or Analysing Biological Materials (AREA)

NO20002540A 1997-12-06 2000-05-16 Isolering av nukleinsyrer NO315323B1 (no)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
GBGB9725839.6A GB9725839D0 (en)	1997-12-06	1997-12-06	Isolation of nucleic acids
GBGB9815541.9A GB9815541D0 (en)	1998-07-17	1998-07-17	Isolation of nucleic acids
PCT/GB1998/003602 WO1999029703A2 (en)	1997-12-06	1998-12-04	Isolation of nucleic acids

Publications (3)

Publication Number	Publication Date
NO20002540D0 NO20002540D0 (no)	2000-05-16
NO20002540L NO20002540L (no)	2000-07-07
NO315323B1 true NO315323B1 (no)	2003-08-18

Family

ID=26312726

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO20002540A NO315323B1 (no)	1997-12-06	2000-05-16	Isolering av nukleinsyrer

Country Status (16)

Country	Link
US (1)	US20030130499A1 (de)
EP (2)	EP1234832B1 (de)
JP (2)	JP2004501054A (de)
KR (2)	KR20010032806A (de)
CN (1)	CN1230440C (de)
AT (2)	ATE218140T1 (de)
AU (1)	AU755342B2 (de)
BR (1)	BR9815569A (de)
CA (1)	CA2318306A1 (de)
DE (2)	DE69805649T2 (de)
DK (1)	DK1036082T3 (de)
ES (2)	ES2177093T3 (de)
HK (1)	HK1034520A1 (de)
NO (1)	NO315323B1 (de)
PT (1)	PT1036082E (de)
WO (1)	WO1999029703A2 (de)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB9425138D0 (en) *	1994-12-12	1995-02-08	Dynal As	Isolation of nucleic acid
US6914137B2 (en) *	1997-12-06	2005-07-05	Dna Research Innovations Limited	Isolation of nucleic acids
JP2002541839A (ja) *	1999-04-21	2002-12-10	アノビス，インコーポレイテッド	植物についての磁気ｄｎａ抽出キット
US6383783B1 (en) *	1999-09-21	2002-05-07	3M Innovative Properties Company	Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
SE9904539D0 (sv) *	1999-12-10	1999-12-10	Alphahelix Ab	Method and device for the handling of samples and reagents
JP2001221721A (ja) *	2000-02-09	2001-08-17	Sapporo Imuno Diagnostic Laboratory:Kk	乾燥濾紙便による遺伝子診断
DE10006590B4 (de) *	2000-02-11	2007-10-18	Qiagen North American Holdings, Inc.	Verwendung funktionalisierter Membranen bzw. Matrizes zur Aufreinigung von Nukleinsäuren sowie entsprechende Verfahren
EP1349951A4 (de) *	2001-01-09	2005-08-24	Whitehead Biomedical Inst	Verfahren und reagenzien zur isolierung von nukleinsäuren
US7829025B2 (en)	2001-03-28	2010-11-09	Venture Lending & Leasing Iv, Inc.	Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en)	2001-03-28	2014-11-25	Handylab, Inc.	Methods and systems for control of general purpose microfluidic devices
US8110351B2 (en)	2002-01-16	2012-02-07	Invitrogen Dynal As	Method for isolating nucleic acids and protein from a single sample
GB0212825D0 (en) *	2002-05-31	2002-07-10	Dna Res Innovations Ltd	Methods compositions and kits for cell separation
GB0212826D0 (en) *	2002-05-31	2002-07-10	Dna Res Innovations Ltd	Materials and methods relating to polyions and substance delivery
GB0229287D0 (en) *	2002-12-16	2003-01-22	Dna Res Innovations Ltd	Polyfunctional reagents
US7601491B2 (en)	2003-02-06	2009-10-13	Becton, Dickinson And Company	Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040197780A1 (en) *	2003-04-02	2004-10-07	Agencourt Bioscience Corporation	Method for isolating nucleic acids
CN100395257C (zh) *	2003-05-08	2008-06-18	慈溪市中鼎生物技术有限公司	钾离子酸性水溶液和利用这种溶液的dna提取方法和试剂盒
EP3718635A1 (de)	2003-07-31	2020-10-07	Handylab, Inc.	Verarbeitung partikelhaltiger proben
US8470586B2 (en) *	2004-05-03	2013-06-25	Handylab, Inc.	Processing polynucleotide-containing samples
US8852862B2 (en)	2004-05-03	2014-10-07	Handylab, Inc.	Method for processing polynucleotide-containing samples
WO2005111246A1 (en) *	2004-05-11	2005-11-24	Wyeth	Oligonucleotide arrays to monitor gene expression and methods for making and using same
US20060024712A1 (en) *	2004-06-25	2006-02-02	Invitrogen Corporation	Separation of nucleic acid
CN101027413B (zh)	2004-07-28	2016-08-17	佳能美国生命科学公司	用于监测生物的基因组dna的方法
EP1776458A2 (de) *	2004-07-30	2007-04-25	Agencourt Bioscience Corporation	Verfahren zur isolierung von nukleinsäuren mit hilfe von festphasenträgern mit multifunktionalen gruppen
CA2575446C (en) *	2004-08-03	2014-03-25	Becton, Dickinson And Company	Use of magnetic material to direct isolation of compounds and fractionation of multipart samples
KR100647306B1 (ko) *	2004-12-23	2006-11-23	삼성전자주식회사	아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
US7964380B2 (en)	2005-01-21	2011-06-21	Argylia Technologies	Nanoparticles for manipulation of biopolymers and methods of thereof
US20060166223A1 (en) *	2005-01-26	2006-07-27	Reed Michael W	DNA purification and analysis on nanoengineered surfaces
KR100647315B1 (ko) *	2005-02-02	2006-11-23	삼성전자주식회사	실란화된 고상 물질을 이용한 핵산의 분리 및 증폭 방법
CN101163800B (zh) *	2005-02-18	2013-04-17	佳能美国生命科学公司	鉴定生物的基因组dna的装置和方法
US20060234251A1 (en) *	2005-04-19	2006-10-19	Lumigen, Inc.	Methods of enhancing isolation of RNA from biological samples
KR100668337B1 (ko)	2005-05-21	2007-01-12	삼성전자주식회사	단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
WO2007028084A2 (en)	2005-09-01	2007-03-08	Canon U.S. Life Sciences, Inc.	Method and molecular diagnostic device for detection, analysis and identification of genomic dna
GB0522193D0 (en)	2005-10-31	2005-12-07	Axis Shield Asa	Method
US20100021974A1 (en) *	2005-12-05	2010-01-28	Hideji Tajima	Method for Preparation of cRNA
US11806718B2 (en)	2006-03-24	2023-11-07	Handylab, Inc.	Fluorescence detector for microfluidic diagnostic system
ES2692380T3 (es)	2006-03-24	2018-12-03	Handylab, Inc.	Método para realizar PCR con un cartucho con varias pistas
US8883490B2 (en)	2006-03-24	2014-11-11	Handylab, Inc.	Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en)	2006-03-24	2011-08-16	Handylab, Inc.	Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en)	2006-03-24	2021-01-26	Handylab, Inc.	Microfluidic system for amplifying and detecting polynucleotides in parallel
KR100785010B1 (ko) *	2006-04-06	2007-12-11	삼성전자주식회사	수소 결합을 이용하여 고체 지지체의 친수성 표면 상에서핵산 정제 방법 및 장치
BRPI0710637A2 (pt) *	2006-04-21	2012-03-13	Wyeth	análise do perfil de expressão diferencial de fenótipos de cultura celular e seus usos
US7608399B2 (en)	2006-06-26	2009-10-27	Blood Cell Storage, Inc.	Device and method for extraction and analysis of nucleic acids from biological samples
US8158411B2 (en)	2006-08-21	2012-04-17	Samsung Electronics Co., Ltd.	Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same
KR100813264B1 (ko) *	2006-08-21	2008-03-13	삼성전자주식회사	비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법
KR100837401B1 (ko) *	2006-08-21	2008-06-12	삼성전자주식회사	비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치
US20080070282A1 (en)	2006-08-21	2008-03-20	Samsung Electronics Co., Ltd.	Method and device for obtaining or amplifying nucleic acid from a cell using a nonplanar solid substrate
KR100813265B1 (ko) *	2006-08-21	2008-03-13	삼성전자주식회사	비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법
WO2008035991A2 (en) *	2006-09-19	2008-03-27	Michael Ronald Cook	A nucleic acid extraction method
GB2445441B (en) *	2006-09-26	2010-06-30	Ge Healthcare Bio Sciences	Nucleic acid purification method
WO2008061165A2 (en) *	2006-11-14	2008-05-22	Handylab, Inc.	Microfluidic cartridge and method of making same
US8999636B2 (en) *	2007-01-08	2015-04-07	Toxic Report Llc	Reaction chamber
GB0701253D0 (en)	2007-01-23	2007-02-28	Diagnostics For The Real World	Nucleic acid amplification and testing
CA2629589C (en) *	2007-04-20	2016-03-29	F.Hoffmann-La Roche Ag	Isolation and purification of nucleic acid molecules with a solid phase
US20090017460A1 (en) *	2007-06-15	2009-01-15	Wyeth	Differential expression profiling analysis of cell culture phenotypes and uses thereof
WO2009009127A2 (en) *	2007-07-12	2009-01-15	U.S. Genomics, Inc.	Method and system for transferring and/or concentrating a sample
US9618139B2 (en)	2007-07-13	2017-04-11	Handylab, Inc.	Integrated heater and magnetic separator
US8287820B2 (en)	2007-07-13	2012-10-16	Handylab, Inc.	Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en)	2007-07-13	2012-01-31	Handylab, Inc.	Microfluidic cartridge
US8182763B2 (en)	2007-07-13	2012-05-22	Handylab, Inc.	Rack for sample tubes and reagent holders
WO2009012185A1 (en)	2007-07-13	2009-01-22	Handylab, Inc.	Polynucleotide capture materials, and methods of using same
US9186677B2 (en)	2007-07-13	2015-11-17	Handylab, Inc.	Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10125388B2 (en)	2007-10-31	2018-11-13	Akonni Biosystems, Inc.	Integrated sample processing system
WO2009086215A2 (en) *	2007-12-21	2009-07-09	Wyeth	Pathway analysis of cell culture phenotypes and uses thereof
WO2009117167A1 (en)	2008-01-02	2009-09-24	Blood Cell Storage, Inc.	Devices and processes for nucleic acid extraction
GB0814570D0 (en)	2008-08-08	2008-09-17	Diagnostics For The Real World	Isolation of nucleic acid
KR101015502B1 (ko)	2008-09-09	2011-02-22	삼성전자주식회사	제1 ｐＨ에서 양전하 및 제2 ｐＨ에서 음전하를 띠는 화합물 및 그를 이용하여 핵산을 분리하는 방법
CN102177237B (zh)	2008-09-12	2013-10-30	金沃特公司	用于贮存和稳定生物分子的基质和介质
US8361716B2 (en) *	2008-10-03	2013-01-29	Pathogenetix, Inc.	Focusing chamber
US20100204462A1 (en)	2008-10-13	2010-08-12	Thomas Walter	Pipette tip with separation material
DE102008063001A1 (de)	2008-12-23	2010-06-24	Qiagen Gmbh	Nukleinsäureaufreinigungsverfahren
DE102008063003A1 (de) *	2008-12-23	2010-06-24	Qiagen Gmbh	Nukleinsäureaufreinigungsverfahren
US9464319B2 (en)	2009-03-24	2016-10-11	California Institute Of Technology	Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9447461B2 (en)	2009-03-24	2016-09-20	California Institute Of Technology	Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
WO2010111265A1 (en)	2009-03-24	2010-09-30	University Of Chicago	Slip chip device and methods
US10196700B2 (en)	2009-03-24	2019-02-05	University Of Chicago	Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
EP2256195A1 (de) *	2009-05-12	2010-12-01	Qiagen GmbH	Nukleinsäureaufreinigungsverfahren
IN2012DN03431A (de)	2009-09-21	2015-10-23	Akonni Biosystems
JP2013513400A (ja) *	2009-12-14	2013-04-22	ベティウー	核酸物質を分離する方法および物質
KR101878749B1 (ko)	2010-03-05	2018-07-17	삼성전자주식회사	표적 세포의 분리 방법 및 키트
EP2596135A2 (de)	2010-07-23	2013-05-29	Beckman Coulter, Inc.	System und verfahren mit analyseeinheiten
EP3709025A1 (de)	2011-04-15	2020-09-16	Becton, Dickinson and Company	Mikrofluidischer abtastender echtzeit-thermocycler und verfahren zur optischen detektion mittels synchronisierter thermozyklierung und abtastung
WO2013004710A2 (en) *	2011-07-04	2013-01-10	Qiagen Gmbh	Reagent usable for the isolation and/or purification of nucleic acids
EP4159857A1 (de)	2011-09-26	2023-04-05	QIAGEN GmbH	Schnelles verfahren zur isolierung extrazellulärer nukleinsäuren
WO2013049706A1 (en)	2011-09-30	2013-04-04	Becton, Dickinson And Company	Unitized reagent strip
WO2013062476A1 (en)	2011-10-27	2013-05-02	Ge Healthcare Bio-Sciences Ab	Purification of nucleic acid
WO2013067202A1 (en)	2011-11-04	2013-05-10	Handylab, Inc.	Polynucleotide sample preparation device
BR112014011044A2 (pt)	2011-11-07	2017-04-25	Beckman Coulter Inc	amortecimento magnético para sistema de transporte de espécime
CN104040352B (zh)	2011-11-07	2016-09-07	贝克曼考尔特公司	机械臂
JP6320926B2 (ja)	2011-11-07	2018-05-09	ベックマンコールター，インコーポレイテッド	遠心分離機システムおよびワークフロー
KR20140092377A (ko)	2011-11-07	2014-07-23	베크만 컬터, 인코포레이티드	분취기 시스템 및 작업흐름
BR112014011043A2 (pt)	2011-11-07	2017-06-13	Beckman Coulter Inc	detecção de recipiente de espécime
CN104053997B (zh)	2011-11-07	2016-12-21	贝克曼考尔特公司	用于处理样本的***和方法
CA2863637C (en)	2012-02-03	2021-10-26	Becton, Dickinson And Company	External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US8685708B2 (en)	2012-04-18	2014-04-01	Pathogenetix, Inc.	Device for preparing a sample
EP2839260B1 (de)	2012-04-20	2018-07-18	Talis Biomedical Corporation	Fluidische vorrichtungen und systeme zur herstellung oder autonomen analyse einer probe
WO2013159116A1 (en)	2012-04-20	2013-10-24	University Of Chicago	Fluidic devices for biospecimen preservation
US9803237B2 (en)	2012-04-24	2017-10-31	California Institute Of Technology	Slip-induced compartmentalization
WO2014035090A1 (ko)	2012-08-28	2014-03-06	주식회사 바이오큐브시스템	생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
US9719082B2 (en) *	2013-10-31	2017-08-01	General Electric Company	Substrates and associated methods for elution of nucleic acids
KR102344395B1 (ko) *	2014-06-24	2021-12-29	(주)바이오니아	자성 입자를 이용한 핵산 분리 방법
CN104513819B (zh) *	2014-12-17	2020-05-05	吉林大学	一种选择性提取dna的方法
CN107690481B (zh)	2015-06-10	2021-08-13	凯杰有限公司	利用阴离子交换粒子分离细胞外核酸的方法
KR101696549B1 (ko) *	2015-07-31	2017-01-13	전북대학교산학협력단	수소이온농도 대응 검체 스왑 장치
KR102190922B1 (ko) *	2015-09-01	2020-12-14	삼성전자주식회사	핵산 분리 방법
WO2017162518A1 (en)	2016-03-19	2017-09-28	Qiagen Gmbh	Stabilization of rna
US11161107B2 (en)	2016-05-25	2021-11-02	Integrated Micro-Chromatography Systems, Inc.	Dispersive pipette extraction system for purification of large biomolecules
CN106520522A (zh) *	2016-12-06	2017-03-22	厦门华厦学院	一种dna萃取装置及方法
EP3502274A1 (de) *	2017-12-22	2019-06-26	Attomol GmbH	Probenträger und verfahren zu dessen herstellung
CN108660074B (zh) *	2018-05-28	2022-04-29	嘉兴市艾科诺生物科技有限公司	核酸提取pcr扩增检测一体化解决方案
EP3916397B1 (de) *	2019-01-25	2023-08-30	Sekisui Chemical Co., Ltd.	Verfahren zur isolierung von nukleinsäure, nukleinsäureisolierungskit und inspektionschip
JP2020124129A (ja) *	2019-02-01	2020-08-20	積水化学工業株式会社	核酸の単離方法及び核酸単離用デバイス
CN110129314B (zh) *	2019-04-24	2021-04-13	合肥欧创基因生物科技有限公司	一种氨基酸缓冲液及血浆游离dna提取试剂盒
CN114845957A (zh) *	2019-09-18	2022-08-02	南科征途有限公司	用于按尺寸富集和分离核酸的设备***和方法
EP4077659A1 (de)	2019-12-16	2022-10-26	QIAGEN GmbH	Anreicherungsverfahren
EP4355873A1 (de)	2021-06-17	2024-04-24	QIAGEN GmbH	Verfahren zur isolierung von nichtvesikulärer mirna

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3797202A (en) *	1971-08-27	1974-03-19	Gen Electric	Microporous/non-porous composite membranes
US4055469A (en) *	1976-12-10	1977-10-25	Eastman Kodak Company	Purification of microbial enzyme extracts using synthetic polyelectrolytes
US4843012A (en) *	1986-09-17	1989-06-27	Genetics Institute, Inc.	Novel composition for nucleic acid purification
DE3639949A1 (de) *	1986-11-22	1988-06-09	Diagen Inst Molekularbio	Verfahren zur trennung von langkettigen nukleinsaeuren
US5599667A (en) *	1987-03-02	1997-02-04	Gen-Probe Incorporated	Polycationic supports and nucleic acid purification separation and hybridization
JPH01125395A (ja) *	1987-07-29	1989-05-17	Life Technol Inc	核酸浦獲試薬
US4921805A (en) *	1987-07-29	1990-05-01	Life Technologies, Inc.	Nucleic acid capture method
US4908318A (en) *	1987-09-04	1990-03-13	Integrated Genetics, Inc.	Nucleic acid extraction methods
US4923978A (en) *	1987-12-28	1990-05-08	E. I. Du Pont De Nemours & Company	Process for purifying nucleic acids
US5234809A (en) *	1989-03-23	1993-08-10	Akzo N.V.	Process for isolating nucleic acid
US5334499A (en) *	1989-04-17	1994-08-02	Eastman Kodak Company	Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5124444A (en) *	1989-07-24	1992-06-23	Microprobe Corporation	Lactam-containing compositions and methods useful for the extraction of nucleic acids
US5128247A (en) *	1989-08-14	1992-07-07	Board Of Regents, The University Of Texas System	Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5433847A (en) *	1989-11-01	1995-07-18	Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College	Radial flow chromatography
US5641628A (en) *	1989-11-13	1997-06-24	Children's Medical Center Corporation	Non-invasive method for isolation and detection of fetal DNA
IT1240870B (it) *	1990-02-14	1993-12-17	Talent	Procedimento per l'estrazione e la purificazione di dna genomico umano
JPH06510363A (ja) *	1990-10-29	1994-11-17	ディカルブ　プラント　ジェネティクス	磁気性粒子を使用する生物学的材料の単離
US5654179A (en) *	1990-11-14	1997-08-05	Hri Research, Inc.	Nucleic acid preparation methods
US5204246A (en) *	1990-12-26	1993-04-20	Pioneer Hi-Bred International, Inc.	Dna isolation method
US6051380A (en) *	1993-11-01	2000-04-18	Nanogen, Inc.	Methods and procedures for molecular biological analysis and diagnostics
US5710028A (en) *	1992-07-02	1998-01-20	Eyal; Nurit	Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
WO1995027718A2 (en) *	1994-04-08	1995-10-19	Hybridon, Inc.	Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
US5622822A (en) *	1994-09-13	1997-04-22	Johnson & Johnson Clinical Diagnostics, Inc.	Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) *	1994-09-15	1996-12-10	Johnson & Johnson Clinical Diagnostics, Inc.	Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705628A (en) *	1994-09-20	1998-01-06	Whitehead Institute For Biomedical Research	DNA purification and isolation using magnetic particles
US5652348A (en) *	1994-09-23	1997-07-29	Massey University	Chromatographic resins and methods for using same
US5660984A (en) *	1994-12-09	1997-08-26	Davis; Thomas E.	DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
US5631146A (en) *	1995-01-19	1997-05-20	The General Hospital Corporation	DNA aptamers and catalysts that bind adenosine or adenosine-5'-phosphates and methods for isolation thereof
US5612473A (en) *	1996-01-16	1997-03-18	Gull Laboratories	Methods, kits and solutions for preparing sample material for nucleic acid amplification
US5981735A (en) *	1996-02-12	1999-11-09	Cobra Therapeutics Limited	Method of plasmid DNA production and purification
SE9600590D0 (sv) *	1996-02-19	1996-02-19	Pharmacia Biotech Ab	Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
US5916746A (en) *	1996-05-09	1999-06-29	Kirkegaard & Perry Laboratories, Inc.	Formazan-based immunoassay
US5981235A (en) *	1996-07-29	1999-11-09	Promega Corporation	Methods for isolating nucleic acids using alkaline protease
JP3793287B2 (ja) *	1996-08-22	2006-07-05	ペンタックス株式会社	プラスミドｄｎａの単離方法
CA2214495C (en) *	1996-09-25	2002-02-05	Daniel L. Woodard	Hydrated zirconium silicate composition for purification of nucleic acids
US6060246A (en) *	1996-11-15	2000-05-09	Avi Biopharma, Inc.	Reagent and method for isolation and detection of selected nucleic acid sequences
US5770712A (en) *	1997-03-14	1998-06-23	Virginia Tech Intellectual Properties, Inc.	Crosslinked hydrogel beads from chitosan
EP0897978A3 (de) *	1997-08-22	2001-10-17	Becton, Dickinson and Company	Zirkoniumoxyd und verwandten Verbindungen zur Reinigung von Nukleinsäuren
JP4304348B2 (ja) *	1997-09-22	2009-07-29	独立行政法人理化学研究所	Ｄｎａの単離方法
DE19743518A1 (de) *	1997-10-01	1999-04-15	Roche Diagnostics Gmbh	Automatisierbare universell anwendbare Probenvorbereitungsmethode
US7078224B1 (en) *	1999-05-14	2006-07-18	Promega Corporation	Cell concentration and lysate clearance using paramagnetic particles
US6194562B1 (en) *	1998-04-22	2001-02-27	Promega Corporation	Endotoxin reduction in nucleic acid purification
US6534262B1 (en) *	1998-05-14	2003-03-18	Whitehead Institute For Biomedical Research	Solid phase technique for selectively isolating nucleic acids
AU2862100A (en) *	1999-01-27	2000-08-18	Folim G. Halaka	Materials and methods for the purification of polyelectrolytes
US6310199B1 (en) *	1999-05-14	2001-10-30	Promega Corporation	pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) *	1999-05-14	2001-08-07	Promega Corporation	Mixed-bed solid phase and its use in the isolation of nucleic acids

1998
- 1998-12-04 BR BR9815569-5A patent/BR9815569A/pt not_active Application Discontinuation
- 1998-12-04 CN CNB988118939A patent/CN1230440C/zh not_active Expired - Lifetime
- 1998-12-04 EP EP02003403A patent/EP1234832B1/de not_active Revoked
- 1998-12-04 AU AU13447/99A patent/AU755342B2/en not_active Ceased
- 1998-12-04 AT AT98957019T patent/ATE218140T1/de not_active IP Right Cessation
- 1998-12-04 KR KR1020007006123A patent/KR20010032806A/ko active Application Filing
- 1998-12-04 ES ES98957019T patent/ES2177093T3/es not_active Expired - Lifetime
- 1998-12-04 KR KR1020057014820A patent/KR20050088164A/ko not_active Application Discontinuation
- 1998-12-04 DE DE69805649T patent/DE69805649T2/de not_active Expired - Lifetime
- 1998-12-04 PT PT98957019T patent/PT1036082E/pt unknown
- 1998-12-04 WO PCT/GB1998/003602 patent/WO1999029703A2/en not_active Application Discontinuation
- 1998-12-04 AT AT02003403T patent/ATE386044T1/de not_active IP Right Cessation
- 1998-12-04 DE DE69839133T patent/DE69839133T2/de not_active Expired - Lifetime
- 1998-12-04 DK DK98957019T patent/DK1036082T3/da active
- 1998-12-04 JP JP2000524296A patent/JP2004501054A/ja active Pending
- 1998-12-04 EP EP98957019A patent/EP1036082B1/de not_active Expired - Lifetime
- 1998-12-04 CA CA002318306A patent/CA2318306A1/en not_active Abandoned
- 1998-12-04 ES ES02003403T patent/ES2301581T3/es not_active Expired - Lifetime
2000
- 2000-05-16 NO NO20002540A patent/NO315323B1/no unknown
2001
- 2001-07-20 HK HK01105089A patent/HK1034520A1/xx not_active IP Right Cessation
2002
- 2002-11-21 US US10/300,990 patent/US20030130499A1/en not_active Abandoned
2010
- 2010-03-08 JP JP2010050067A patent/JP2010162037A/ja active Pending

Also Published As

Publication number	Publication date
KR20050088164A (ko)	2005-09-01
BR9815569A (pt)	2001-10-09
DK1036082T3 (da)	2002-08-12
ES2177093T3 (es)	2002-12-01
JP2010162037A (ja)	2010-07-29
AU1344799A (en)	1999-06-28
JP2004501054A (ja)	2004-01-15
DE69805649D1 (de)	2002-07-04
CN1230440C (zh)	2005-12-07
EP1036082B1 (de)	2002-05-29
US20030130499A1 (en)	2003-07-10
WO1999029703A2 (en)	1999-06-17
PT1036082E (pt)	2002-10-31
WO1999029703A3 (en)	1999-08-26
NO20002540D0 (no)	2000-05-16
KR20010032806A (ko)	2001-04-25
ATE218140T1 (de)	2002-06-15
AU755342B2 (en)	2002-12-12
NO20002540L (no)	2000-07-07
DE69839133T2 (de)	2009-02-05
ATE386044T1 (de)	2008-03-15
ES2301581T3 (es)	2008-07-01
EP1234832A3 (de)	2003-08-13
EP1036082A2 (de)	2000-09-20
HK1034520A1 (en)	2001-10-26
CA2318306A1 (en)	1999-06-17
EP1234832B1 (de)	2008-02-13
DE69839133D1 (de)	2008-03-27
EP1234832A2 (de)	2002-08-28
DE69805649T2 (de)	2002-12-05
CN1281462A (zh)	2001-01-24

Publication	Publication Date	Title
NO315323B1 (no)	2003-08-18	Isolering av nukleinsyrer
JP4868697B2 (ja)	2012-02-01	核酸の分離
US20090215124A1 (en)	2009-08-27	Nucleic acid isolation methods and materials and devices thereof
WO2000070041A1 (en)	2000-11-23	Mixed-bed solid phase and its use in the isolation of nucleic acids
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WO2015154013A1 (en)	2015-10-08	System and method for dna extraction from samples containing humic acids
JP5463492B2 (ja)	2014-04-09	微生物細胞からのプラスミドｄｎａ抽出法
MXPA00005474A (en)	2001-07-03	Isolation of nucleic acids
Gautam	2022	Spin column-based isolation of nucleic acid
CN1781931A (zh)	2006-06-07	核酸的分离
US20230142197A1 (en)	2023-05-11	Cation exchange for sperm-associated dna purification
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