JPWO2012032844A1 - 細胞培養容器及びその容器を用いた細胞培養方法 - Google Patents
細胞培養容器及びその容器を用いた細胞培養方法 Download PDFInfo
- Publication number
- JPWO2012032844A1 JPWO2012032844A1 JP2012532891A JP2012532891A JPWO2012032844A1 JP WO2012032844 A1 JPWO2012032844 A1 JP WO2012032844A1 JP 2012532891 A JP2012532891 A JP 2012532891A JP 2012532891 A JP2012532891 A JP 2012532891A JP WO2012032844 A1 JPWO2012032844 A1 JP WO2012032844A1
- Authority
- JP
- Japan
- Prior art keywords
- well
- gas
- cell culture
- flow path
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 59
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 238000009826 distribution Methods 0.000 claims abstract description 17
- 239000001301 oxygen Substances 0.000 claims description 92
- 229910052760 oxygen Inorganic materials 0.000 claims description 92
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 91
- 239000007789 gas Substances 0.000 claims description 87
- 239000007788 liquid Substances 0.000 claims description 14
- 239000000758 substrate Substances 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 60
- 206010028980 Neoplasm Diseases 0.000 description 29
- 201000011510 cancer Diseases 0.000 description 25
- 238000000034 method Methods 0.000 description 10
- 238000004088 simulation Methods 0.000 description 10
- 210000004204 blood vessel Anatomy 0.000 description 7
- 210000000988 bone and bone Anatomy 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 239000007850 fluorescent dye Substances 0.000 description 6
- 210000000130 stem cell Anatomy 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- NPRDEIDCAUHOJU-UHFFFAOYSA-N [Pt].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 Chemical compound [Pt].N1C(C=C2N=C(C=C3NC(=C4)C=C3)C=C2)=CC=C1C=C1C=CC4=N1 NPRDEIDCAUHOJU-UHFFFAOYSA-N 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 239000006143 cell culture medium Substances 0.000 description 4
- 239000004205 dimethyl polysiloxane Substances 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 239000012298 atmosphere Substances 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 208000032839 leukemia Diseases 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- -1 polydimethylsiloxane Polymers 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 229920006367 Neoflon Polymers 0.000 description 1
- 206010029113 Neovascularisation Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000002798 bone marrow cell Anatomy 0.000 description 1
- 230000005907 cancer growth Effects 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 229920006129 ethylene fluorinated ethylene propylene Polymers 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 238000012576 optical tweezer Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000000963 osteoblast Anatomy 0.000 description 1
- 210000002997 osteoclast Anatomy 0.000 description 1
- 150000002926 oxygen Chemical class 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 210000000603 stem cell niche Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- DCGLONGLPGISNX-UHFFFAOYSA-N trimethyl(prop-1-ynyl)silane Chemical compound CC#C[Si](C)(C)C DCGLONGLPGISNX-UHFFFAOYSA-N 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
この実施例の細胞培養容器1はウエル5、第1流路10及び第2流路12を内部に備えている。ウエル5は直方体形状の空間であり、その対向する一対の側面の一方の側面に第1流路10の一部区間がガス透過膜14を介して接し、反対側の側面に第2流路12の一部区間がガス透過膜16を介して接している。ガス透過膜14,16は、ガスは通すが液体を通さない膜である。ウエル5の他の一対の側面の一方の側面には細胞培養液の導入用流路6、他方の側面には排出用流路8を備えている。
2,4 透明基板
3 流路形成シート
5 ウエル
6 導入用流路
8 排出用流路
10 第1流路
12 第2流路
14,16 ガス透過膜(多孔質膜)
18,20,22,24,26,28 貫通孔
Claims (8)
- 基体内部に形成され、細胞を収容する空間をもち、細胞培養液を流通させる流路に繋がり、前記空間の対向する一対の側面の一方が気体を透過させ液体を透過させない第1ガス透過膜からなり、前記一対の側面の他方が気体を透過させ液体を透過させない第2ガス透過膜からなるウエルと、
前記第1ガス透過膜を介して前記ウエルと接し、特定成分を含むガスを流通させる第1流路と、
前記第2ガス透過膜を介して前記ウエルと接し、特定成分を含まないか又は前記第1流路に流すガスよりも特定成分を低濃度に含むガスを流通させる第2流路と、を備えた細胞培養容器。 - 前記ウエルは6.3×10-6mm3から1mm3の間の容積を有する請求項1に記載の細胞培養容器。
- 前記ウエルの上下面を封止している上下面封止部の少なくとも一方が外部から前記ウエル内部を視認可能な透明窓となっている請求項1又は2に記載の細胞培養容器。
- 一方の前記透明窓の内面に、接触液中の酸素濃度に応じて光学的性質を変化させる酸素モニタ物質が固定されている請求項3に記載の細胞培養容器。
- 請求項3に記載の細胞培養容器のウエルに細胞培養液を充填するとともに細胞を導入するステップと、
前記ウエルに導入された細胞のウエル内における停止位置を前記透明窓を介して認識するステップと、
前記第1流路及び第2流路のガス流量とウエル内に形成される特定成分の濃度分布との予め求められた関係に基づいて、細胞の停止位置における前記特定成分の濃度を所定濃度にするために第1流路及び第2流路のガス流量を設定するステップと、
前記ウエル内の細胞培養液は静止させたままで、前記第1流路及び第2流路に前記ガス流量設定ステップで設定したガス流量でガスを流すステップと、
を備えて細胞の培養を行なう細胞培養方法。 - 前記特定成分は酸素である請求項5に記載の細胞培養方法。
- 前記ウエル内の酸素濃度は21%未満である請求項6に記載の細胞培養方法。
- 前記細胞はiPS細胞であり、細胞の停止位置における酸素濃度を約5%にする請求項7に記載の細胞培養方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012532891A JP5703302B2 (ja) | 2010-09-08 | 2011-07-08 | 細胞培養容器及びその容器を用いた細胞培養方法 |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010200679 | 2010-09-08 | ||
JP2010200679 | 2010-09-08 | ||
JP2012532891A JP5703302B2 (ja) | 2010-09-08 | 2011-07-08 | 細胞培養容器及びその容器を用いた細胞培養方法 |
PCT/JP2011/065662 WO2012032844A1 (ja) | 2010-09-08 | 2011-07-08 | 細胞培養容器及びその容器を用いた細胞培養方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2012032844A1 true JPWO2012032844A1 (ja) | 2014-01-20 |
JP5703302B2 JP5703302B2 (ja) | 2015-04-15 |
Family
ID=45810449
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012532891A Active JP5703302B2 (ja) | 2010-09-08 | 2011-07-08 | 細胞培養容器及びその容器を用いた細胞培養方法 |
Country Status (4)
Country | Link |
---|---|
US (2) | US20130164848A1 (ja) |
JP (1) | JP5703302B2 (ja) |
CN (1) | CN103080294B (ja) |
WO (1) | WO2012032844A1 (ja) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013046980A1 (ja) * | 2011-09-27 | 2013-04-04 | 株式会社島津製作所 | 細胞選別器及び細胞選別方法 |
KR20150095699A (ko) * | 2012-12-12 | 2015-08-21 | 히타치가세이가부시끼가이샤 | 암 세포 단리 장치 및 암 세포 단리 방법 |
CN108165603A (zh) | 2013-01-25 | 2018-06-15 | 艾克斯赛尔生物科学公司 | 用于选择性富集靶细胞的方法、组合物、试剂盒及*** |
US9790465B2 (en) | 2013-04-30 | 2017-10-17 | Corning Incorporated | Spheroid cell culture well article and methods thereof |
JP6227425B2 (ja) * | 2014-01-17 | 2017-11-08 | アズビル株式会社 | 複数種類の粒子の蛍光強度の比較方法及び蛍光検出装置の評価方法 |
EP3212759A1 (en) | 2014-10-29 | 2017-09-06 | Corning Incorporated | Cell culture insert |
SG11201703500XA (en) * | 2014-10-29 | 2017-05-30 | Corning Inc | Perfusion bioreactor platform |
JP7197557B2 (ja) | 2017-07-14 | 2022-12-27 | コーニング インコーポレイテッド | 多孔性支持体を有する細胞培養槽 |
US11857970B2 (en) | 2017-07-14 | 2024-01-02 | Corning Incorporated | Cell culture vessel |
JP7245222B2 (ja) | 2017-07-14 | 2023-03-23 | コーニング インコーポレイテッド | 手動又は自動で培地を交換するための3d細胞培養容器 |
WO2019014610A1 (en) | 2017-07-14 | 2019-01-17 | Corning Incorporated | CELL CULTURE CONTAINER FOR 3D CULTURE AND METHODS OF CULTURING 3D CELLS |
US20210207072A1 (en) * | 2018-05-29 | 2021-07-08 | Hitachi High-Tech Corporation | Cell detection device and cell detection method |
US11912968B2 (en) | 2018-07-13 | 2024-02-27 | Corning Incorporated | Microcavity dishes with sidewall including liquid medium delivery surface |
US11661574B2 (en) | 2018-07-13 | 2023-05-30 | Corning Incorporated | Fluidic devices including microplates with interconnected wells |
PL3649229T3 (pl) | 2018-07-13 | 2021-12-06 | Corning Incorporated | Naczynia do hodowli komórkowych ze stabilizującymi urządzeniami |
JP2020110117A (ja) * | 2019-01-15 | 2020-07-27 | 株式会社島津製作所 | 細胞処理容器及び細胞処理装置 |
CN111575184B (zh) * | 2020-05-21 | 2022-11-15 | 上海理工大学 | 可以控制细胞培养环境溶氧水平的微流控芯片及制作方法 |
WO2024092347A1 (en) * | 2022-10-31 | 2024-05-10 | Val-Chum, Limited Partnership | Cell culture devices |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5416022A (en) * | 1988-08-10 | 1995-05-16 | Cellex Biosciences, Inc. | Cell culture apparatus |
CA2422110A1 (en) * | 2000-09-18 | 2002-03-21 | President And Fellows Of Harvard College | Method and apparatus for gradient generation |
JP2004329122A (ja) * | 2003-05-08 | 2004-11-25 | Jgc Corp | 細胞培養装置および培養方法 |
US8658349B2 (en) * | 2006-07-13 | 2014-02-25 | Seahorse Bioscience | Cell analysis apparatus and method |
CN101346167B (zh) * | 2005-12-28 | 2011-04-13 | 株式会社岛津制作所 | 压力差气泡移动控制方法以及使用了该方法的气体交换装置、导电率测定装置、总有机碳测定装置、反应装置及细胞培养装置 |
US7575890B2 (en) * | 2006-01-18 | 2009-08-18 | Oxygen Enterprises, Ltd. | Method for rapid detection and evaluation of cultured cell growth |
US7611204B2 (en) * | 2006-06-30 | 2009-11-03 | Caterpillar Inc. | Rotary adjustment mechanism |
WO2008028241A1 (en) * | 2006-09-06 | 2008-03-13 | The University Of Queensland | Microbioreactor |
US8501462B2 (en) * | 2007-02-27 | 2013-08-06 | The Board Of Trustees Of The University Of Illinois | Insert device for multiwell plate |
-
2011
- 2011-07-08 JP JP2012532891A patent/JP5703302B2/ja active Active
- 2011-07-08 WO PCT/JP2011/065662 patent/WO2012032844A1/ja active Application Filing
- 2011-07-08 CN CN201180041898.1A patent/CN103080294B/zh active Active
- 2011-07-08 US US13/821,063 patent/US20130164848A1/en not_active Abandoned
-
2018
- 2018-12-28 US US16/235,432 patent/US20190136171A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO2012032844A1 (ja) | 2012-03-15 |
JP5703302B2 (ja) | 2015-04-15 |
CN103080294A (zh) | 2013-05-01 |
US20190136171A1 (en) | 2019-05-09 |
CN103080294B (zh) | 2017-02-15 |
US20130164848A1 (en) | 2013-06-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5703302B2 (ja) | 細胞培養容器及びその容器を用いた細胞培養方法 | |
Zirath et al. | Every breath you take: non-invasive real-time oxygen biosensing in two-and three-dimensional microfluidic cell models | |
Oomen et al. | Implementing oxygen control in chip-based cell and tissue culture systems | |
Brennan et al. | Oxygen control with microfluidics | |
Günther et al. | A microfluidic platform for probing small artery structure and function | |
Lesher-Pérez et al. | Dispersible oxygen microsensors map oxygen gradients in three-dimensional cell cultures | |
US20150247112A1 (en) | 3D Tissue Culture Devices and Systems | |
Rivera et al. | Measuring and regulating oxygen levels in microphysiological systems: Design, material, and sensor considerations | |
US10731119B2 (en) | Method and devices for the in vitro production of arrangements of cell layers | |
WO2008028241A1 (en) | Microbioreactor | |
US20180066220A1 (en) | Devices for cell culture and methods of making and using the same | |
Grant et al. | Establishment of physiologically relevant oxygen gradients in microfluidic organ chips | |
Chiang et al. | Polydimethylsiloxane-polycarbonate microfluidic devices for cell migration studies under perpendicular chemical and oxygen gradients | |
Wu et al. | Review of microfluidic cell culture devices for the control of gaseous microenvironments in vitro | |
Bunge et al. | Microfluidic oxygen sensor system as a tool to monitor the metabolism of mammalian cells | |
EP2841941B1 (en) | Methods for determining and/or monitoring conditions of a three dimensional cell culture system and optical sensor device for conducting said methods | |
Liu et al. | Microfluidic liquid-air dual-gradient chip for synergic effect bio-evaluation of air pollutant | |
Gil et al. | Cancer Models on Chip: Paving the Way to Large‐Scale Trial Applications | |
Bunge et al. | PDMS-free microfluidic cell culture with integrated gas supply through a porous membrane of anodized aluminum oxide | |
US10793820B2 (en) | Miniaturized, automated in-vitro tissue bioreactor | |
Shuchat et al. | Perfusion in Organ-on-Chip Models and Its Applicability to the Replication of Spermatogenesis In Vitro | |
Lee et al. | Design criteria and standardization of a microfluidic cell culture system for investigating cellular migration | |
JP2010011814A (ja) | 培養細胞観察用チャンバー及びその使用 | |
TW202039819A (zh) | 細胞培養晶片 | |
Eroğlu | In vitro 3D Spheroid Culture Developed on the Parafilm Surface Using HEK-293 Cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140722 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140901 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150210 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150223 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5703302 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |