JPS585198A - クレアチニン検定のための自動連続流れ法 - Google Patents

クレアチニン検定のための自動連続流れ法

Info

Publication number: JPS585198A
Authority: JP; Japan
Prior art keywords: reagent; creatinine; ammonia; reagents; substances
Prior art date: 1981-06-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP57108634A

Other languages

English (en)

Japanese (ja)

Inventor

ステフア−ノ・カムビアギ

ダニエレ・バツシ

テイツイア−ノ・ベネツキ

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Pfizer Italia SRL

Original Assignee

Carlo Erba SpA

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-06-26

Filing date

1982-06-25

Publication date

1983-01-12

1982-06-25 Application filed by Carlo Erba SpA filed Critical Carlo Erba SpA

1983-01-12 Publication of JPS585198A publication Critical patent/JPS585198A/ja

Status Pending legal-status Critical Current

Links

DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 title claims description 188
229940109239 creatinine Drugs 0.000 title claims description 94
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 158
239000003153 chemical reaction reagent Substances 0.000 claims description 101
229910021529 ammonia Inorganic materials 0.000 claims description 79
238000000034 method Methods 0.000 claims description 70
229920001778 nylon Polymers 0.000 claims description 33
239000004677 Nylon Substances 0.000 claims description 32
108090000790 Enzymes Proteins 0.000 claims description 24
102000004190 Enzymes Human genes 0.000 claims description 24
239000000243 solution Substances 0.000 claims description 24
238000000502 dialysis Methods 0.000 claims description 23
239000000126 substance Substances 0.000 claims description 23
VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 19
108700016171 Aspartate ammonia-lyases Proteins 0.000 claims description 18
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 18
238000003379 elimination reaction Methods 0.000 claims description 16
230000008030 elimination Effects 0.000 claims description 15
101000833181 Schizosaccharomyces pombe (strain 972 / ATCC 24843) Glycerol dehydrogenase 1 Proteins 0.000 claims description 14
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 13
239000000872 buffer Substances 0.000 claims description 13
238000006243 chemical reaction Methods 0.000 claims description 13
238000001514 detection method Methods 0.000 claims description 13
239000008363 phosphate buffer Substances 0.000 claims description 13
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 12
238000003556 assay Methods 0.000 claims description 12
239000005708 Sodium hypochlorite Substances 0.000 claims description 10
SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 10
238000012360 testing method Methods 0.000 claims description 9
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 8
239000011734 sodium Substances 0.000 claims description 8
229910052708 sodium Inorganic materials 0.000 claims description 8
RHYBFKMFHLPQPH-UHFFFAOYSA-N N-methylhydantoin Chemical compound CN1CC(=O)NC1=O RHYBFKMFHLPQPH-UHFFFAOYSA-N 0.000 claims description 7
239000013060 biological fluid Substances 0.000 claims description 7
WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 claims description 6
238000005259 measurement Methods 0.000 claims description 6
KPGXRSRHYNQIFN-UHFFFAOYSA-N 2-oxoglutaric acid Chemical compound OC(=O)CCC(=O)C(O)=O KPGXRSRHYNQIFN-UHFFFAOYSA-N 0.000 claims description 5
101710155216 Methylamine dehydrogenase heavy chain Proteins 0.000 claims description 4
101710183893 Methylamine dehydrogenase light chain Proteins 0.000 claims description 4
239000002253 acid Substances 0.000 claims description 4
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 3
229910019142 PO4 Inorganic materials 0.000 claims description 3
230000002255 enzymatic effect Effects 0.000 claims description 3
239000000463 material Substances 0.000 claims description 3
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
239000010452 phosphate Substances 0.000 claims description 3
230000004913 activation Effects 0.000 claims description 2
230000029936 alkylation Effects 0.000 claims description 2
238000005804 alkylation reaction Methods 0.000 claims description 2
239000007788 liquid Substances 0.000 claims description 2
150000003839 salts Chemical class 0.000 claims description 2
239000012224 working solution Substances 0.000 claims 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims 3
229940049920 malate Drugs 0.000 claims 2
BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims 2
241000218691 Cupressaceae Species 0.000 claims 1
VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 1
239000005977 Ethylene Substances 0.000 claims 1
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical group [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
239000007853 buffer solution Substances 0.000 claims 1
239000003431 cross linking reagent Substances 0.000 claims 1
230000001186 cumulative effect Effects 0.000 claims 1
238000007872 degassing Methods 0.000 claims 1
FRTGEIHSCHXMTI-UHFFFAOYSA-N dimethyl octanediimidate Chemical compound COC(=N)CCCCCCC(=N)OC FRTGEIHSCHXMTI-UHFFFAOYSA-N 0.000 claims 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-O ethanol;hydron Chemical compound CC[OH2+] LFQSCWFLJHTTHZ-UHFFFAOYSA-O 0.000 claims 1
229940014144 folate Drugs 0.000 claims 1
OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims 1
235000019152 folic acid Nutrition 0.000 claims 1
239000011724 folic acid Substances 0.000 claims 1
239000008055 phosphate buffer solution Substances 0.000 claims 1
239000011574 phosphorus Substances 0.000 claims 1
229910052698 phosphorus Inorganic materials 0.000 claims 1
239000004033 plastic Substances 0.000 claims 1
239000004071 soot Substances 0.000 claims 1
210000002966 serum Anatomy 0.000 description 14
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 12
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 12
238000011534 incubation Methods 0.000 description 8
210000002700 urine Anatomy 0.000 description 8
101710088194 Dehydrogenase Proteins 0.000 description 7
238000004458 analytical method Methods 0.000 description 7
238000006297 dehydration reaction Methods 0.000 description 5
230000000694 effects Effects 0.000 description 5
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
239000007983 Tris buffer Substances 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
230000018044 dehydration Effects 0.000 description 4
239000002207 metabolite Substances 0.000 description 4
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
210000004369 blood Anatomy 0.000 description 3
239000008280 blood Substances 0.000 description 3
230000003197 catalytic effect Effects 0.000 description 3
230000001419 dependent effect Effects 0.000 description 3
238000010586 diagram Methods 0.000 description 3
235000018102 proteins Nutrition 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
238000007430 reference method Methods 0.000 description 3
229930024421 Adenine Natural products 0.000 description 2
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 2
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
108010046334 Urease Proteins 0.000 description 2
229960000643 adenine Drugs 0.000 description 2
229910000147 aluminium phosphate Inorganic materials 0.000 description 2
239000003146 anticoagulant agent Substances 0.000 description 2
229940127219 anticoagulant drug Drugs 0.000 description 2
230000037213 diet Effects 0.000 description 2
235000005911 diet Nutrition 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
-1 ethyloxonium tetrafluoroborate Chemical compound 0.000 description 2
230000007717 exclusion Effects 0.000 description 2
229930195712 glutamate Natural products 0.000 description 2
230000007062 hydrolysis Effects 0.000 description 2
238000006460 hydrolysis reaction Methods 0.000 description 2
230000000977 initiatory effect Effects 0.000 description 2
239000011591 potassium Substances 0.000 description 2
229910052700 potassium Inorganic materials 0.000 description 2
238000011084 recovery Methods 0.000 description 2
239000012086 standard solution Substances 0.000 description 2
ZNEMGFATAVGQSF-UHFFFAOYSA-N 1-(2-amino-6,7-dihydro-4H-[1,3]thiazolo[4,5-c]pyridin-5-yl)-2-[5-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]-1,3,4-oxadiazol-2-yl]ethanone Chemical compound NC=1SC2=C(CN(CC2)C(CC=2OC(=NN=2)C=2C=NC(=NC=2)NC2CC3=CC=CC=C3C2)=O)N=1 ZNEMGFATAVGQSF-UHFFFAOYSA-N 0.000 description 1
FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 description 1
SFRDXVJWXWOTEW-UHFFFAOYSA-N 2-(hydroxymethyl)propane-1,3-diol Chemical compound OCC(CO)CO SFRDXVJWXWOTEW-UHFFFAOYSA-N 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
208000025299 Atresia of urethra Diseases 0.000 description 1
101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 1
241000951471 Citrus junos Species 0.000 description 1
206010010219 Compulsions Diseases 0.000 description 1
XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 1
QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
206010018367 Glomerulonephritis chronic Diseases 0.000 description 1
102000016901 Glutamate dehydrogenase Human genes 0.000 description 1
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
206010067350 Hypercreatinaemia Diseases 0.000 description 1
108010093096 Immobilized Enzymes Proteins 0.000 description 1
LFVLUOAHQIVABZ-UHFFFAOYSA-N Iodofenphos Chemical compound COP(=S)(OC)OC1=CC(Cl)=C(I)C=C1Cl LFVLUOAHQIVABZ-UHFFFAOYSA-N 0.000 description 1
125000001176 L-lysyl group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C([H])([H])C([H])([H])C([H])([H])C(N([H])[H])([H])[H] 0.000 description 1
240000000249 Morus alba Species 0.000 description 1
235000008708 Morus alba Nutrition 0.000 description 1
239000001747 Potassium fumarate Substances 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
206010064895 Urethral atresia Diseases 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
230000001154 acute effect Effects 0.000 description 1
238000000137 annealing Methods 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
229940009098 aspartate Drugs 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
230000001588 bifunctional effect Effects 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
238000005112 continuous flow technique Methods 0.000 description 1
238000012937 correction Methods 0.000 description 1
238000012258 culturing Methods 0.000 description 1
238000000354 decomposition reaction Methods 0.000 description 1
239000013530 defoamer Substances 0.000 description 1
238000006356 dehydrogenation reaction Methods 0.000 description 1
238000011161 development Methods 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
VTYGSWGUBDXCRA-UHFFFAOYSA-L dipotassium;2-oxopentanedioate Chemical compound [K+].[K+].[O-]C(=O)CCC(=O)C([O-])=O VTYGSWGUBDXCRA-UHFFFAOYSA-L 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000011010 flushing procedure Methods 0.000 description 1
239000001530 fumaric acid Substances 0.000 description 1
230000006870 function Effects 0.000 description 1
159000000011 group IA salts Chemical class 0.000 description 1
229960002897 heparin Drugs 0.000 description 1
229920000669 heparin Polymers 0.000 description 1
238000005286 illumination Methods 0.000 description 1
230000003100 immobilizing effect Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
210000003734 kidney Anatomy 0.000 description 1
208000017169 kidney disease Diseases 0.000 description 1
230000003907 kidney function Effects 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
229910052753 mercury Inorganic materials 0.000 description 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
230000002906 microbiologic effect Effects 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000004118 muscle contraction Effects 0.000 description 1
201000008383 nephritis Diseases 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
MAYUCBCSAVDUKG-UHFFFAOYSA-N orthoacetic acid Chemical compound CC(O)(O)O MAYUCBCSAVDUKG-UHFFFAOYSA-N 0.000 description 1
238000006213 oxygenation reaction Methods 0.000 description 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
150000003017 phosphorus Chemical class 0.000 description 1
OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
SHPKCSFVQGSAJU-SEPHDYHBSA-L potassium fumarate Chemical compound [K+].[K+].[O-]C(=O)\C=C\C([O-])=O SHPKCSFVQGSAJU-SEPHDYHBSA-L 0.000 description 1
235000019295 potassium fumarate Nutrition 0.000 description 1
159000000001 potassium salts Chemical class 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
238000005086 pumping Methods 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
238000007127 saponification reaction Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
125000006850 spacer group Chemical group 0.000 description 1
238000012289 standard assay Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
229940014800 succinic anhydride Drugs 0.000 description 1
239000004094 surface-active agent Substances 0.000 description 1
230000002123 temporal effect Effects 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
239000002023 wood Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

JP57108634A 1981-06-26 1982-06-25 クレアチニン検定のための自動連続流れ法 Pending JPS585198A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
IT22575A/81		1981-06-26
IT22575/81A IT1137262B (it)	1981-06-26	1981-06-26	Metodo automatico su flusso continuo per il dosaggio della creatinina con creatinina desimidasi immobilizzata

Publications (1)

Publication Number	Publication Date
JPS585198A true JPS585198A (ja)	1983-01-12

Family

ID=11197999

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP57108634A Pending JPS585198A (ja)	1981-06-26	1982-06-25	クレアチニン検定のための自動連続流れ法

Country Status (5)

Country	Link
JP (1)	JPS585198A (de)
DE (1)	DE3220548A1 (de)
FR (1)	FR2508488B1 (de)
GB (1)	GB2102568B (de)
IT (1)	IT1137262B (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CS238261B1 (en) *	1983-07-28	1985-11-13	Jiri Cerkasov	Enzymatic reactions execution and tracing method and device for application of this method
JPS6156095A (ja) *	1984-08-27	1986-03-20	Kyowa Hakko Kogyo Co Ltd	基質または酵素活性の定量法
JPH0664056B2 (ja) *	1987-04-16	1994-08-22	富士写真フイルム株式会社	アンモニア生成物質定量用一体型多層分析要素
US5804452A (en) *	1995-04-27	1998-09-08	Quidel Corporation	One step urine creatinine assays

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4134793A (en) *	1975-08-28	1979-01-16	Kyowa Hakko Kogyo Co., Ltd.	Creatinine desimidase in the quantitative determination of creatinine
US4276377A (en) *	1979-11-05	1981-06-30	Eastman Kodak Company	Creatinine iminohydrolase free from urease activity

1981
- 1981-06-26 IT IT22575/81A patent/IT1137262B/it active
1982
- 1982-06-01 DE DE19823220548 patent/DE3220548A1/de not_active Withdrawn
- 1982-06-24 GB GB08218264A patent/GB2102568B/en not_active Expired
- 1982-06-25 FR FR8211184A patent/FR2508488B1/fr not_active Expired
- 1982-06-25 JP JP57108634A patent/JPS585198A/ja active Pending

Also Published As

Publication number	Publication date
IT8122575A0 (it)	1981-06-26
GB2102568B (en)	1984-11-07
FR2508488B1 (fr)	1985-09-06
IT1137262B (it)	1986-09-03
GB2102568A (en)	1983-02-02
DE3220548A1 (de)	1983-01-20
FR2508488A1 (fr)	1982-12-31

Publication	Publication Date	Title
Apstein et al.	1970	Improved automated lactate determination
US7002670B2 (en)	2006-02-21	Optical sensor and method for measuring concentration of a chemical constituent using its intrinsic optical absorbance
CN107957495B (zh)	2020-10-09	一种ck-mb检测试剂盒及其使用方法
JPS63294799A (ja)	1988-12-01	グルコ−ス及び１，５−アンヒドログルシト−ルの同時測定法
CN112029817A (zh)	2020-12-04	一种肌酐检测试剂盒及其使用方法
CA1229781A (en)	1987-12-01	Method for determination of ammonia
JPS585198A (ja)	1983-01-12	クレアチニン検定のための自動連続流れ法
Sundaram et al.	1978	Immobilized-enzyme nylon-tube reactors for routine determination of urea and citrulline in serum.
CN106885803B (zh)	2019-06-28	一种抗干扰能力强且准确度高的酶法乙醇检测试剂
Chirillo et al.	1979	The use of immobilized enzyme reactors in continuous-flow analyzers for the determination of glucose, urea, and uric acid.
Fujimura et al.	1981	Microdetermination of galactose and galactose 1-phosphate in dried blood spots
CN111190003A (zh)	2020-05-22	一种视黄醇结合蛋白检测试剂盒及其制备方法
Werner et al.	1979	Immobilized enzymes in continuous-flow analysis.
Collison et al.	1987	Continuous-flow enzymatic determination of creatinine with improved on-line removal of endogenous ammonia
CN109187389A (zh)	2019-01-11	一种海洋低温尿酸氧化酶测定尿酸的检测试剂盒
CN110699422B (zh)	2022-07-26	一种基于金纳米簇荧光增强的乳酸检测方法
Murachi et al.	1988	[23] Use of immobilized enzyme column reactors in clinical analysis
Tritsch et al.	1975	Automated determination of adenosine aminohydrolase in human plasma and erythrocytes by continuous flow analysis
Yasuhara et al.	1981	Continuous-flow enzymic determination of creatine in urine.
CN116124770A (zh)	2023-05-16	一种基于双酶-无机杂化微球的二胺检测试剂盒及其制备方法和应用
Kamoun et al.	1978	Immobilized-enzyme membrane sandwich reactor used in automated micro-scale assay of plasma uric acid.
Nakajima et al.	1985	Enzymic determination of methylguanidine in urine
WO2011108670A1 (ja)	2011-09-09	タウリンの分析方法
Stalikas et al.	1994	Flow injection determination of glutamate in human serum and rat brain samples with immobilized glutamate oxidase and glutamate dehydrogenase reactors
Hinsch et al.	1981	Harnsäurebestimmung mit Hilfe eines Aldehyddehy-drogenase-und eines Uricase-Aldehyddehydrogenase-Nylonschlauch-Reaktors