JPH06277043A - Culture of pachyma hoelen rumph - Google Patents

Abstract

PURPOSE:To artificially culture Pachyma hoelen Rumph. CONSTITUTION:5g of glucose, 8g of malt extract, 0.3g of CSL, 5g of dry yeast, 3g of (NH4)2SO4, 0.1g of CaCl2.6H2O, and 0.1g of thiamine are dissolved in 11 of ion-exchanged water to form a liquid medium. The raw fungus of the Pachyma hoelen Rumph is inoculated into the liquid medium and subsequently subjected to a stationary culture at a temperature of 20 deg.C. L-Tryptophan is added to the culture solution at the 13th days after the inoculation of the raw fungus, and the stationary culture is further continued. The amount of the Pacyhma hoelen Rumph obtained by the culture increases with the passage of the culturing dates, thus enabling to artificially culture the Pachyma hoelen Rumph by this method.

Description

【発明の詳細な説明】Detailed Description of the Invention

【０００１】[0001]

【産業上の利用分野】本発明は、茯苓の培養方法に関す
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for cultivating a scale.

【０００２】[0002]

【従来の技術】近年薬害の問題から副作用の恐れの少な
い漢方薬（生薬）が見直されてきており、その使用量は
増大の一途である。そのうち茯苓は不完全菌類に属する
ものであるが、安魂、養神、延年、利小便やその他多く
の作用を有し、種々の症状に有効な優れた漢方薬の成分
として知られている。2. Description of the Related Art In recent years, Chinese herbs (herbal medicines), which are less likely to cause side effects due to the problem of drug damage, have been reviewed, and the amount used has been increasing. Of these, Furei, which belongs to the incomplete fungi, has many effects such as Ansoul, Yojin, Renewal, urine and urine, and is known as an ingredient of an excellent herbal medicine effective for various symptoms.

【０００３】この茯苓は伐採した松の切り株の３〜５年
を経た根に寄生した菌核であり、全形のものは丸みのあ
る大小不定の塊で重さ１〜２ｋｇに達するが通常はその
断片又は切片から成る。従来かかる茯苓を採取するに
は、茯苓は土中の深さ１０〜３０ｃｍ程の枯れた松の根
の周囲に生ずるため、その様な場所を目当てとして、Ｔ
字型の柄に鉄製の先の尖った棒を取り付けてなる茯苓突
きを用いて土中を突いて探す方法が採られていた。この
方法では、茯苓が存在する土中に茯苓突きを突き刺した
時には必ず白色の茯苓片が付着し、また刺した感触が異
なることに基づいて茯苓の存在を確認している。This rooster is a sclerotium that parasitizes the roots of a felled pine stump aged 3 to 5 years, and the whole one is a rounded large and small indefinite mass that weighs 1 to 2 kg. It consists of fragments or pieces. Conventionally, in order to collect such a flesh flesh, since the flesh flesh occurs around a dead pine root with a depth of about 10 to 30 cm in the soil, aiming for such a place, T
A method was used in which the iron was used to attach a pointed iron rod to the handle to make a search for it by plunging into the soil. According to this method, when a peony stake is stabbed in the soil where a skein flesh is present, a white skein flee piece is always attached, and the existence of a flee peony is confirmed based on the different feeling of the pierce.

【０００４】[0004]

【発明が解決しようとしている課題】しかしながら上述
の茯苓の採取方法は、茯苓突きを持って枯れた松の根の
周辺を突いて歩く作業であり大変な労力がかかる上に、
茯苓の存在の確認には熟練を要するため採取が困難であ
るという問題があった。また最近では、宅地開発に伴い
茯苓の生育場所が減少すると共に、採取専門家が高年齢
化し、茯苓の採取が益々困難になってきている。従って
茯苓の国内採取量は需要量に追いつかず、その大部分は
中国から輸入されているのが現状である。However, the above-mentioned method of collecting a peony is a work in which a peony butt is used to poke and walk around a dead pine root.
There is a problem that it is difficult to collect because the skill is required to confirm the existence of the peony. In addition, recently, along with the development of residential land, the number of peony growing places has decreased, and the collection specialists have become older, making it even more difficult to collect peony. Therefore, the domestic collection volume of Peony cannot keep up with the demand volume, and most of it is currently imported from China.

【０００５】本発明はこのような事情のもとになされた
ものであり、その目的は茯苓を人工的に培養する方法を
提供することにある。The present invention has been made under such circumstances, and an object thereof is to provide a method for artificially cultivating a scale.

【０００６】[0006]

【課題を解決するための手段】本発明者は、種々の固型
培養や液体培養を試行錯誤的に行って、茯苓の培養を試
みた結果、本発明方法を確立するに至った。Means for Solving the Problems The inventors of the present invention have established the method of the present invention as a result of trial and error of various solid-type cultures and liquid cultures to try to culture peony.

【０００７】即ち、本発明は、糖類と、穀類の芽または
胚芽からの抽出成分と、ビタミンとを含む液体培地を用
い、この液体培地に培養の途中からアミノ酸を添加する
ことを特徴とする。That is, the present invention is characterized in that a liquid medium containing sugars, components extracted from cereal buds or germs, and vitamins is used, and amino acids are added to the liquid medium during the culture.

【０００８】[0008]

【作用】糖類と、穀類の芽または胚芽からの抽出成分
と、ビタミンとを含む液体培地を作成し、茯苓の原菌を
接種した後、この液体培地に培養の途中からアミノ酸を
添加する。かかる培地を用いて静止培養を行うことによ
り茯苓は発育するので、人工的に茯苓を培養することが
できる。[Function] A liquid medium containing saccharides, components extracted from cereal buds or germs, and vitamins is prepared, inoculated with the stocks of the peony, and amino acids are added to the liquid medium during the culture. By performing stationary culture using such a medium, since the scales grow, the scales can be artificially cultured.

【０００９】[0009]

【実施例】本発明の茯苓の培養方法は、水に例えばグル
コースからなる糖類と、例えば麦芽のエキスであるmalt
extractやトウモロコシの胚芽のエキスであるＣＳＬ等
の穀類の芽または胚芽のエキスと、例えばdry yeast か
らなるビタミンとを溶解して得た液体培地に茯苓の原菌
を接種し、接種後１０〜２０日の間に、この液体培地に
例えばトリプトファンやアントラニル酸、アルギニン等
のアミノ酸を添加して、温度１５〜２８℃の下で、静止
培養により、茯苓を培養するものである。EXAMPLES The method for cultivating a peony according to the present invention comprises a saccharide such as glucose in water and malt, which is an extract of malt, for example.
Inoculate a liquid medium obtained by dissolving a cereal bud or germ extract such as CSL, which is an extract of corn germ extract, and a vitamin composed of, for example, dry yeast, and inoculate the broth of the scorpion into 10 to 20 after inoculation. During the day, tryptophan, amino acids such as anthranilic acid, and arginine are added to this liquid medium, and the scorpion is cultured by static culturing at a temperature of 15 to 28 ° C.

【００１０】以下に本発明の液体培地による培養方法を
用いて実際に茯苓の培養を試みた実験例について説明す
る。An experimental example of actually attempting to culture 苀 苋 will be described below using the culture method using the liquid medium of the present invention.

【００１１】［実験例］ （方法）イオン交換水１ｌに、グルコース５ｇ、malt e
xtract８ｇ、ＣＳＬ０．３ｇ、dry yeast ５ｇ、（ＮＨ
₄ ）₂ ＳＯ₄ ３ｇ、ＣａＣｌ₂ ・６Ｈ₂ Ｏ０．１ｇ、チ
アミン０．１ｇを溶解し（〈表１〉実験例１参照）、液
体培地を作成した。５００ｍｌ片口ルー氏フラスコに、
上述の液体培地を３００ｍｌ入れ、茯苓の原菌を１白金
耳接種した後密栓し、培養温度２０℃の下で、静止培養
を行った。この後原菌接種後１３日目にＬ−トリプトフ
ァン０．０５ｇを培地に添加し、引き続き静止培養を行
った。[Experimental Example] (Method) 1 g of ion-exchanged water, 5 g of glucose and malt e
xtract8g, CSL0.3g, dry yeast 5g, (NH
₄ ) ₂ SO ₄ 3 g, CaCl ₂ .6H ₂ O 0.1 g, and thiamine 0.1 g were dissolved (see Experimental Example 1 in Table 1) to prepare a liquid medium. In a 500 ml one-neck Rou flask,
300 ml of the above-mentioned liquid medium was placed, 1 platinum loop of a stock of Bombyx mori was inoculated, then sealed, and static culture was performed at a culture temperature of 20 ° C. Then, 13 days after the inoculation of the protozoan, 0.05 g of L-tryptophan was added to the medium, and then stationary culture was carried out.

【００１２】３０日放置後ルー氏フラスコから茯苓を取
り出し、乾燥した後計量を行った。この操作を１０日毎
に６０日経過するまで行った（実験例１）。また〈表
１〉の実験例２、３に示すように、培養の途中から液体
培地に添加するアミノ酸や培地の組成を変えて同様の実
験を行った。なお実験例２においてアントラニル酸は原
菌接種後１５日目に、実験例３においてＬ−アルギニン
は原菌接種後１３日目にそれぞれ添加した。After standing for 30 days, the scale was taken out from the Roux flask, dried and weighed. This operation was performed every 10 days until 60 days passed (Experimental Example 1). In addition, as shown in Experimental Examples 2 and 3 of <Table 1>, similar experiments were performed while changing the composition of the amino acid added to the liquid medium and the medium during the culture. In Experimental Example 2, anthranilic acid was added 15 days after the inoculation of the original bacterium, and in Experimental Example 3, L-arginine was added 13 days after the inoculation of the original bacterium.

【００１３】[0013]

【表１】 （結果）上述の［実験例１〜３］の結果、即ち本発明の
培養方法により培養された茯苓の量を〈表２〉に示す。
なおこの値は、液体培地１ｌから得られる茯苓の量に換
算したものである。[Table 1] (Results) Table 2 shows the results of the above-mentioned [Experimental Examples 1 to 3], that is, the amount of the sardines cultivated by the culturing method of the present invention.
In addition, this value is converted into the amount of Fukuryo obtained from 1 liter of the liquid medium.

【００１４】[0014]

【表２】 次に本発明の茯苓の培養方法を確立するまでに試行錯誤
的に行った培養方法を比較例として説明する。本発明の
培養方法を見出すまでは種々の固型培養や液体培養を行
ったが、比較例としてはこれらのうちからその一部であ
る鋸屑による固型培養（比較例１）、potato dextrose
培地による液体培養（比較例２）、無機成分を主体とす
る液体培養（比較例３）について記載する。[Table 2] Next, a culture method carried out by trial and error until establishing the method for cultivating a peony according to the present invention will be described as a comparative example. Various solid-type cultures and liquid cultures were carried out until the culture method of the present invention was found. As comparative examples, solid-type culture with sawdust, which is a part of these, (Comparative Example 1), potato dextrose.
Liquid culture with a medium (Comparative Example 2) and liquid culture mainly containing inorganic components (Comparative Example 3) will be described.

【００１５】［比較例１−鋸屑による固型培養］ （方法）鋸屑１０００ｇ、米糠３００ｇ、ウイスキー粕
１０ｇを混合して固型培地を作成し（〈表３〉組成１参
照）、８００ｍｌエノキ茸栽培養ビンを用いて、茯苓の
培養を行った。また培地成分の混合量を、〈表３〉の組
成２〜４に示す量に変えて同様の実験を行った。[Comparative Example 1-Solid culture with sawdust] (Method) 1000 g of sawdust, 300 g of rice bran, and 10 g of whiskey lees were mixed to prepare a solid medium (see composition 1 in Table 3), and 800 ml of Enoki mushroom was cultivated. The peony culturing was carried out using a feeding bottle. Further, the same experiment was conducted by changing the mixing amount of the medium components to the amounts shown in the compositions 2 to 4 of Table 3.

【００１６】[0016]

【表３】 （結果）原菌を接種後約１０日で菌糸の発育が見られた
が、６０日経過してもそれ以上の発育は認められなかっ
た。[Table 3] (Results) Growth of mycelia was observed about 10 days after inoculation with the protozoa, but no further growth was observed even after 60 days.

【００１７】［比較例２−potato dextrose 培地による
液体培養］ （方法）馬鈴薯３００ｇを水約８００ｍｌで煮た後濾過
して濾液を得、この濾液を１ｌの量になるまで水で希釈
することにより液体培地を作成し、上述の［実験例］と
同様の方法を用いて茯苓の液体培養を行った。 （結果）茯苓の発育は認められなかった。[Comparative Example 2-Liquid culture with potato dextrose medium] (Method) 300 g of potatoes was boiled with about 800 ml of water and filtered to obtain a filtrate, and the filtrate was diluted with water to a volume of 1 liter. A liquid culture medium was prepared, and a liquid culture of the scorpion was performed using the same method as in the above [Experimental Example]. (Results) No development of peony was observed.

【００１８】［比較例３−無機成分を主体とする液体培
養］ （方法）１ｌの水に、無機成分としてＫＨ₂ ＰＯ₄ １
ｇ、（ＮＨ₄ ）₂ ＳＯ₄ ２ｇ、ＭｇＳＯ₄ ０．１ｇ、Ｆ
ｅＳＯ₄ ０．２ｇ、ＫＣｌ０．５ｇ、ＮａＮＯ₃ １ｇを
溶解して液体培地を作成し（〈表４〉の組成１参照）、
上述の［実験例］と同様の方法を用いて茯苓の培養を行
った。また培地の組成を〈表４〉の組成２、３に示す値
に変えて同様の実験を行った。さらに〈表５、６〉に示
すように無機成分を種々変えたり、〈表７〉に示すよう
に例えばグルコース等の糖分を加える等、液体培地の成
分や組成を変えて同様の実験を行った。[Comparative Example 3-Liquid culture mainly composed of inorganic components] (Method) In 1 l of water, KH ₂ PO ₄ 1 as an inorganic component
g, (NH ₄ ) ₂ SO ₄ 2 g, MgSO ₄ 0.1 g, F
A liquid medium was prepared by dissolving 0.2 g of eSO ₄ , 0.5 g of KCl, and 1 g of NaNO ₃ (see composition 1 in Table 4),
Falcon was cultivated using the same method as in the above [Experimental Example]. Further, the same experiment was conducted by changing the composition of the medium to the values shown in Compositions 2 and 3 of Table 4. Further, similar experiments were carried out by changing the components and composition of the liquid medium, such as changing various inorganic components as shown in <Tables 5 and 6> and adding sugars such as glucose as shown in <Table 7>. .

【００１９】[0019]

【表４】 [Table 4]

【００２０】[0020]

【表５】 [Table 5]

【００２１】[0021]

【表６】 [Table 6]

【００２２】[0022]

【表７】 （結果）いずれの場合も、原菌を接種後４０日の段階で
は、発芽は全くしないか、あるいはほとんどしない状態
であることが確認された。[Table 7] (Results) In any case, it was confirmed that no germination or almost no germination was observed at the stage of 40 days after the inoculation of the protozoa.

【００２３】次に上述の［実験例］と［比較例］につい
て考察する。茯苓の人工的な培養方法について研究する
にあたり、茯苓は不完全菌類であるため、まず最初に菌
類の一般的な培養方法である鋸屑による固型培養に着目
した。このため上述の［比較例１］の実験を試みたが、
好ましい結果は得られなかった。また茯苓は松の根に寄
生した菌核であることを考慮して、鋸屑の中に松のエキ
スや松やにを加えた培地を用いて同様の実験を行ったが
効果はなかった。Next, the above-mentioned [Experimental example] and [Comparative example] will be considered. In studying the artificial culture method of 苯 苓, since 苯 苓 is an incomplete fungus, first of all, we focused on the solid culture with sawdust, which is a general culturing method of fungi. Therefore, the experiment of [Comparative Example 1] described above was tried,
No favorable result was obtained. Considering that Furei is a sclerotium parasitizing the roots of pine trees, the same experiment was conducted using a medium containing pine extract or pine nuts in sawdust, but there was no effect.

【００２４】次に［比較例２］に示すpotato dextrose
培地による茯苓の液体培地を試みた。この培養方法に着
目したのは、potato dextrose の固体培地が、例えばシ
イタケ、エノキタケ、ヒラタケ、マンネンタケ等の担子
菌類の保存培地として使用されているからである。但し
これらの菌類は、固体培地の表面上において菌糸が発育
するものであるため、茯苓のような地下において発育す
るものは、培養にかかる労力や場所等を考慮すると、液
体中において培養できれば便利であると考えたからであ
る。なおpotato dextrose の液体培地による培養方法
は、主として細菌や藻類の培養に用いられる方法であ
り、糸状菌や担子菌類の培養には用いられていない。し
かしこの方法を用いても好ましい結果は得られなかっ
た。Next, potato dextrose shown in [Comparative Example 2]
An attempt was made to use a liquid medium of Furei on the medium. The reason why this culture method was focused is that the solid medium of potato dextrose is used as a storage medium for basidiomycetes such as shiitake mushroom, enokitake mushroom, oyster mushroom, ganoderma lucidum, and the like. However, since these fungi grow hyphae on the surface of the solid medium, those that grow underground such as 苓苓 will be convenient if they can be cultivated in a liquid in consideration of labor and place for culturing. Because I thought there was. The culture method of potato dextrose in a liquid medium is a method mainly used for culturing bacteria and algae, and is not used for culturing filamentous fungi and basidiomycetes. However, favorable results were not obtained even using this method.

【００２５】［比較例３］に示す無機成分による液体培
養は、主に特殊な栄養要求のある細菌、糸状菌、藻類等
の培養に用いられる方法である。〈表４〜７〉からも明
らかなように、種々の無機成分や糖分を用いて液体培地
を作成し、茯苓の培養を試みたが茯苓を発育させること
に対しほとんど効果はなかった。The liquid culture with the inorganic components shown in [Comparative Example 3] is a method mainly used for culturing bacteria, filamentous fungi, algae and the like having special nutritional requirements. As is clear from <Tables 4 to 7>, a liquid medium was prepared using various inorganic components and sugars, and an attempt was made to culture peony, but there was almost no effect on the development of peony.

【００２６】しかしながらこの培地に、これの添加によ
りある種の植物病原菌（糸状菌）の菌糸の肥大を得たと
いう発明者の過去の実験資料に基づいて、malt extract
を加えたところ茯苓は発芽し、上述の比較例に比べて顕
著な効果が見られたが、培養により得られた茯苓の量は
わずかであり実用性にはほど遠かった。このため穀類の
エキスに注目し、培地にＣＳＬを加えたところ更に発育
が良くなり、またビタミンの一種であるdry yeast を加
えると一層発育が良くなった。さらにグルコース等の糖
分を加えると茯苓の菌糸の発育にさらに良い効果を与え
ることが確認された。However, malt extract was added to this medium based on the past experimental data of the inventor that the hyphae of certain phytopathogenic fungi (filamentous fungi) were obtained by adding this medium.
When added, the sprouting germinated, and a remarkable effect was seen as compared with the above-mentioned comparative example, but the amount of scorpion obtained by the culture was small and it was far from practical use. Therefore, paying attention to grain extracts, when CSL was added to the medium, the growth was further improved, and when dry yeast, which is a kind of vitamin, was added, the growth was further improved. Furthermore, it was confirmed that the addition of sugars such as glucose gives a better effect on the development of the hyphae of the peony.

【００２７】ここで担子菌類はアミノ酸やビタミン類を
含有するものが多いため、アミノ酸の添加により成長を
促進することができるのではないかと考え、アミノ酸に
着目し、培地に添加して茯苓の培養を行ってみた。この
ときの培地の組成を〈表８〉に示す。使用したアミノ酸
は、アルギニン、シスチン、ヒスチジン、グルタミン
酸、メチオニンであり、このうち２種または３種を組み
合わせて添加した。Since many basidiomycetes contain amino acids and vitamins, it may be possible to promote growth by the addition of amino acids. Focusing on the amino acids, adding them to the medium and cultivating the peony I went to The composition of the medium at this time is shown in Table 8. The amino acids used were arginine, cystine, histidine, glutamic acid and methionine, of which two or three were added in combination.

【００２８】[0028]

【表８】 しかしこの培養方法では、培養の初期段階で菌が増殖す
ることは認められたが、その後の茯苓の発育に対しては
よい結果は得られなかった。そこでアミノ酸を培地に最
初から添加すると、菌が初期段階で増殖し過ぎてその後
飢餓状態に近い状態になると考え、アミノ酸を培養の途
中から添加してみたところ茯苓の発育を促進することが
認められた。なお実験によりアミノ酸の添加時期は原菌
接種後１０日〜２０日程度が好ましいと思われる。[Table 8] However, in this culturing method, although it was confirmed that the bacteria grew in the early stage of the culturing, it did not give good results for the subsequent development of the scorpion. Therefore, when amino acids were added to the medium from the beginning, it was thought that the bacteria would overgrow at an early stage and then become in a state of starvation, and addition of amino acids from the middle of the culture was found to promote the development of peony. It was According to the experiment, it is considered preferable to add the amino acid preferably about 10 to 20 days after the inoculation of the protozoa.

【００２９】このようにして種々の固型培養や液体培地
を試行錯誤的に試みた結果、水に無機成分と共に、グル
コース等の糖分、malt extractやＣＳＬ等の穀類の芽ま
たは胚芽からのエキスやdry yeast 等のビタミンを溶解
して得た液体培地が茯苓の培養に適しており、さらにこ
の培地に培養途中からアミノ酸を添加することが茯苓の
発育を促進させることを見出し、従来菌類の培養には用
いられていなかった液体培地を用いた茯苓の培養方法を
確立するに至った。As a result of trial-and-error trials of various solid cultures and liquid media, sugars such as glucose, extracts from cereal buds or germs such as malt extract and CSL were added to water together with inorganic components. A liquid medium obtained by dissolving vitamins such as dry yeast is suitable for cultivating the scorpion, and it was found that adding an amino acid to this medium during the culturing promotes the growth of the scorpion. Came to establish a method for cultivating Fukuryo using a liquid medium that had not been used.

【００３０】このような前提の下で、［実験例］ではグ
ルコース、malt extract、ＣＳＬ、dry yeast の他に、
（ＮＨ₄ ）₂ ＳＯ₄ 、ＣａＣｌ₂ ・６Ｈ₂ Ｏ、ＺｎＳＯ
₄ ・７Ｈ₂ Ｏ等の無機成分やチアミンからなるビタミン
を培地成分とし、これらを〈表１〉に示すように組み合
わせて［実験例１〜３］の３種類の液体培地を作成し、
それぞれの培地にＬ−トリプトファン、アントラニル
酸、Ｌ−アルギニンの三種類のアミノ酸を添加して、こ
れらの培地における茯苓の発育状態を観察した。ちなみ
にＬ−トリプトファンはα−アミノ酸、アントラニル酸
はο−アミノ安息香酸、Ｌ−アルギニンは塩基性α−ア
ミノ酸である。Under such a premise, in [Experimental Example], in addition to glucose, malt extract, CSL and dry yeast,
(NH ₄ ) ₂ SO ₄ , CaCl ₂ .6H ₂ O, ZnSO
₄ · 7H and ₂ O inorganic component and consisting of thiamine vitamin medium components such as, by combining them as shown in <Table 1> create three liquid medium [Experimental Examples 1 to 3,
Three types of amino acids, L-tryptophan, anthranilic acid, and L-arginine were added to each medium, and the developmental condition of the boiled scales in these mediums was observed. By the way, L-tryptophan is an α-amino acid, anthranilic acid is an o-aminobenzoic acid, and L-arginine is a basic α-amino acid.

【００３１】この結果は〈表２〉に示す通りであり、い
ずれの実験例においても培養日数の経過ごとに培養によ
り得られた茯苓の量は増加していることから、茯苓は各
培地中で発育していることが認められ、この方法によっ
て茯苓を人工的に培養できることが確認された。The results are shown in Table 2 and, in any of the experimental examples, the amount of the scorpion flesh obtained by the culturing increased with the lapse of the number of culturing days. It was confirmed that they were growing, and it was confirmed that this method allows artificial cultivation of the 耊 苓.

【００３２】また〈表２〉より、各培地において培養さ
れた茯苓の量を比較すると、培養日数が５０日程度では
大きな違いは見られないが、６０日経過時には［実験例
２］の培地における培養量は他の培地に比べて劣ってい
る。ここで［実験例１］と［実験例２］の培養条件の違
いは、アミノ酸の種類のみである。従って培養途中で添
加するアミノ酸としてＬ−トリプトファンとアントラニ
ル酸とを比較した場合には、茯苓の培養にはＬ−トリプ
トファンが適していると推察される。Further, from Table 2, when comparing the amounts of the boiled peony cultivated in each medium, no significant difference was observed when the number of days of culturing was about 50 days, but after 60 days, in the medium of [Example 2] The culture volume is inferior to other culture media. Here, the difference between the culture conditions of [Experimental Example 1] and [Experimental Example 2] is only the type of amino acid. Therefore, when L-tryptophan and anthranilic acid are compared as the amino acids added during the culture, it is presumed that L-tryptophan is suitable for the cultivation of the 苯 苓.

【００３３】さらに本実施例の効果、すなわち茯苓の発
育に対するアミノ酸の影響を確認するために、［実験例
３］の液体培地と同様の組成を持つ培地を作成し、アミ
ノ酸を添加しない以外は［実験例３］と同条件の下で茯
苓の培養を行った。この培養方法によって得られた茯苓
の量は〈表９〉に示す通りであるが、この値と［実験例
３］の方法によって得られた茯苓の量（〈表２〉参照）
とを比較すると、培養日数が６０日経過時には、アミノ
酸を添加した場合は、アミノ酸を添加しない場合に比べ
て得られた茯苓の量は約２倍となっている。このことか
ら培養途中からアミノ酸を添加することが茯苓の発育を
促進させることが確認された。Further, in order to confirm the effect of the present example, that is, the effect of amino acids on the development of the flesh peony, a medium having the same composition as the liquid medium of [Experimental Example 3] was prepared, and no amino acid was added. Under the same conditions as in [Experimental example 3], cultivated peony was cultured. The amount of peony obtained by this culturing method is as shown in <Table 9>, and this value and the amount of peony obtained by the method of [Experimental Example 3] (see <Table 2>)
Comparing with the above, after 60 days of culturing, when the amino acid was added, the amount of peony obtained was about twice as much as when the amino acid was not added. From this, it was confirmed that the addition of amino acids during the culture promotes the development of the 苋 苓.

【００３４】[0034]

【表９】 なお以上において本発明の茯苓の培養に用いられる液体
培地に含まれる成分としては、グルコース以外の糖類
や、malt extractやＣＳＬ以外の穀類の芽または胚芽か
らの抽出成分、dry yeast 以外のビタミン、トリプトフ
ァン、アントラニル酸、アルギニン以外のアミノ酸を用
いることも可能である。[Table 9] In the above, the components contained in the liquid medium used for cultivating the scales of the present invention include sugars other than glucose, extract components from cereal buds or germs other than malt extract and CSL, vitamins other than dry yeast, tryptophan. It is also possible to use amino acids other than anthranilic acid and arginine.

【００３５】[0035]

【発明の効果】本発明は、糖類、穀類の芽または胚芽か
らの抽出成分、ビタミンを含む液体培地を用い、この液
体培地に培養途中からアミノ酸を添加する液体培養が、
茯苓の培養に適していることを見出したものであり、こ
の培養方法を用いることによって茯苓を人工的に培養す
ることができる。INDUSTRIAL APPLICABILITY The present invention uses a liquid medium containing a sugar, an extract component from a cereal bud or germ, and a vitamin, and a liquid culture in which an amino acid is added to the liquid medium during the culture.
It has been found that it is suitable for cultivating a peony. By using this culturing method, a peony can be artificially cultured.

Claims (1)

【特許請求の範囲】[Claims] 【請求項１】 糖類と、穀類の芽または胚芽からの抽出
成分と、ビタミンとを含む液体培地を用い、この液体培
地に培養の途中からアミノ酸を添加することを特徴とす
る茯苓の培養方法。1. A method for cultivating a peony, characterized in that a liquid medium containing a sugar, an extract component from a cereal bud or embryo and a vitamin is used, and an amino acid is added to the liquid medium during the culture.