JP7189137B2 - シャシャンボ実の抽出物を含む憂鬱症予防及び改善用健康機能性食品組成物 - Google Patents
シャシャンボ実の抽出物を含む憂鬱症予防及び改善用健康機能性食品組成物 Download PDFInfo
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
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Description
図1は、シャシャンボ実抽出物を得る過程を示す。シャシャンボ実2.0kgに蒸溜水30Lを加え、還流抽出機を用いて100℃で温度が上がった後、3時間の間加熱、抽出した。得られた抽出物を減圧フィルタ後、減圧濃縮して107.02gの濃縮液を得た。
図2に図示したように、前記シャシャンボ実の熱水抽出物から系統分画を実施してシャシャンボ実抽出物の分画物を製造するステップを具体的に説明すると、次の通りである。濃縮液を水で十分に懸濁して2倍体積のヘキサンで1次溶媒移行を3回遂行して水層とヘキサン(hexane) ext.(41.8mg)に分離した。また、水層を2倍体積のクロロホルムで2次溶媒移行を3回遂行してCHCl3ext.(79.9mg)を確保した。エチルアセテートで3次溶媒移行を3回遂行してEtOAc ext.(312.4mg)を確保した。最後に、n-ブタノールで4次溶媒移行を3回遂行してn-BuOH ext.(5g)を確保した。
シャシャンボ抽出物の抗憂鬱効果測定のための実験動物として生後6週齢の雄ICRマウスを(株)サムタコ(SAMTACO、Korea)から購入して動物飼育室で一定の条件(温度:22±2℃、湿度:50±5%、明暗:12時間light/dark cycle)で一週間の間適応させた後、使用した。
雄ICRマウスに本発明のシャシャンボ抽出物を経口投与し、比較群には抗憂鬱剤に使われるエスシタロプラムシュウ酸塩を経口投与した。これらは実験によって6日投与した。
正常な状態で憂鬱症と関連した神経伝達物質であるNEとストレスホルモンであるCORの濃度変化に及ぼす影響は、各薬物6日投与後、マウスから血漿(plasma)を分離してAbnova ELISA kitを使用して製造社の指示に従って測定した。
SH-SY5Y cell(neuroblastoma、human dopaminergic neuronal cell)を1% antimycotics/antibioticsと10%FBSを含有したMEM培地で培養し、96-well plateに細胞数が105cell/mlになるようにシードした後、24時間培養した。
CHO-K1細胞株(Chinese Hamster Ovary Cell)をATCCから購入し、培養液(RPMI 1640、fetal bovine serum 10%、penicilin100IU/ml、streptomycin 100μg/ml)を使用して96-well black wall/clear bottom(BD Falcon)に細胞を分株した後、37℃、5% CO2 インキュベーター(incubator)で24時間培養した。セロトニン1A受容体(5-HT1A receptor)またはセロトニン2A受容体(5-HT2A receptor)各々cDNAをプラスミドトランスフェクション試薬であるlipofectamine 2000を用いて48時間の間細胞内一時的に発現させた細胞を実験に使用した。
CHO-K1細胞株(Chinese Hamster Ovary Cell)をATCCから購入し、培養液(RPMI 1640、fetal bovine serum 10%、penicilin 100IU/ml、streptomycin 100μg/ml)を使用して96-well black wall/clear bottom(BD Falcon)に細胞を分株した後、37℃、5%CO2 インキュベーター(incubator)で24時間培養した。セロトニン2A受容体(5-HT2A receptor)各々cDNAをプラスミドトランスフェクション試薬であるlipofectamine 2000を用いて48時間の間細胞内に一時的に発現させた細胞を実験に使用した。
シャシャンボ実抽出物を有効成分とする免疫増強用薬学的組成物または健康食品組成物は錠剤及びカプセル剤、軟質カプセル剤、顆粒剤、液剤形態に製造できる。更に他の適切な実施形態によると、前記組成物は飲料添加剤に製造することができる。前記免疫増強用薬剤または健康食品は、前記シャシャンボ実抽出物を有効成分として含む組成物が0.01~99.9重量%で含まれるように散剤、顆粒剤、錠剤、カプセル剤、懸濁液、エマルジョン、シロップ、エアゾール、経皮剤、坐剤、または滅菌注射溶液に剤形化して製造することができる。
Claims (3)
- シャシャンボ(Vaccinium bracteatum Thunb.)実抽出物を有効成分として含み、
前記シャシャンボ実抽出物が、水抽出物のエチルアセテート又はブタノール分画物である憂鬱症の予防または改善用健康機能性食品組成物。 - 請求項1の健康機能性食品組成物が0.01~99.9重量%の量で含まれることを特徴とする、憂鬱症の予防または改善用健康機能性食品。
- 健康機能性食品は、錠剤、カプセル剤、軟質カプセル剤、顆粒剤、及び液剤のうちから選択されるいずれか1つの形態に製造されることを特徴とする、請求項2に記載の憂鬱症の予防または改善用健康機能性食品。
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KR102388980B1 (ko) | 2021-04-08 | 2022-04-22 | 재단법인 전남바이오산업진흥원 | 모새나무(Vaccinium bacteaum Thunb.) 잎 추출물을 유효성분을 포함하는 피부 장벽, 보습 개선 및 예방용 화장료 조성물 |
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