JP5467433B2 - 海藻発酵組成物およびその製造方法 - Google Patents
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Landscapes
- Edible Seaweed (AREA)
- Seasonings (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Description
発酵食品には、有史以前からの長い歴史があり、その優れた利点から、世界各地に多種多様な発酵食品が発展してきた。発酵食品の例としては、チーズやヨーグルト、ワイン、清酒、味噌、醤油、魚醤、納豆、糠漬け、ザワークラウト、キムチ、鮒寿司、豆腐よう等が挙げられ、今日の食卓において欠かせないものとなっている。
1) ワカメ及びコンブからなる群から選択される海藻類をプロテアーゼで処理し、Pediococcus acidilactici、Lactobacillus curvatus、Leuconostoc mesenteroides subsp. mesenteroides 及び Pediococcus pentosaceus からなる群から選択される乳酸菌により発酵させることを特徴とする海藻発酵組成物。
2) グルタミン酸を20mg/dl以上、乳酸を70mg/dl以上含有する、上記1記載の海藻発酵組成物。
3) ワカメ及びコンブからなる群から選択される海藻類をプロテアーゼで処理し、Pediococcus acidilactici、Lactobacillus curvatus、Leuconostoc mesenteroides subsp. mesenteroides 及び Pediococcus pentosaceus からなる群から選択される乳酸菌により発酵させることを特徴とする海藻発酵組成物の製造方法。
4) 上記1または2記載の海藻発酵組成物を利用して得られる飲食品。
を提供するものである。
(海藻類)
(海藻原料液)
(酵素分解)
(乳酸発酵)
(発酵組成物)
食品に広く用いられている乳酸菌487株を用いて、海藻発酵組成物を調整した。各乳酸菌を、40mlのMRS培地にて30℃で20時間静置培養後、遠心分離にて菌体を分取し、それぞれに30%グリセリンを添加した50%MRS培地を加えて、菌体懸濁液を得た。次に、粉末ワカメ(理研ビタミン社製)200mgおよびプロテアーゼ(天野エンザイム社製)200mgに、滅菌水を総量20mlとなるよう加えて得られる粉末ワカメ水溶液20mlに、菌体懸濁液0.1mlを加え、30℃で48時間発酵し、発酵組成物を得た。各海藻発酵組成物のL−乳酸量およびグルタミン酸量を、バイオセンサーBF−5(王子計測社製)を用いて測定した。その代表例を表1に示す。
海藻をプロテアーゼまたはセルラーゼで処理して得られる組成物中に含まれるグルコース、タンパクの遊離量を測定した。
海藻原料液として、粉砕した干しコンブ(南茅部産)0.2gまたは0.6gを水20mlに加えて撹拌して1%および3%粉末コンブ水溶液を作製し、実施例2に記載の方法に従い、各水溶液をプロテアーゼまたはセルラーゼで処理後、各酵素処理後の水溶液中に含まれるグルコースおよびタンパクの遊離量を算出した。その結果を表6に示す。
粉末ワカメ(理研ビタミン社製)0.2gまたは粉砕した干しコンブ(南茅部産)0.2gを、水20mlにそれぞれ加えて撹拌した。当該海藻原料液に、終濃度が1.0%となるようプロテアーゼを添加した後、さらに菌体懸濁液0.1mlを用いて乳酸菌を接種した後、30℃で48時間培養し、本発明である海藻発酵組成物(本発明8、9)を得た。乳酸菌としては、Leuconostoc mesenteroides subsp.mesenteroidesを用いた。比較例としては、海藻原料液として全量20mlとなる1%粉末ワカメ水溶液、1%粉末コンブ水溶液を用い、セルラーゼまたはプロテアーゼを終濃度1%となるよう添加し、乳酸菌を接種せずに、30℃、48時間の加温後に得られる組成物(比較例11,13,14,16)および各海藻原料液に終濃度が1.0%なるようセルラーゼを添加し、乳酸菌を接種後、30℃で48時間発酵して得られる組成物(比較例12,15)を用いた。アミノ酸量は、実施例1に記載の方法と同様に測定した。
海藻発酵組成物の官能評価試験を行った。官能評価試験に用いた本発明の海藻発酵組成物および比較例の組成物は、実施例2に記載の方法で得た。官能評価試験は、5人のパネラーに対するブラインドテストにより実施し、酸味および旨味についての評価を行った。評価は、各サンプルの相対的な評価を平均化することで算出した。その結果を表9に示す。
Claims (4)
- ワカメ及びコンブからなる群から選択される海藻類をプロテアーゼで処理し、Pediococcus acidilactici、Lactobacillus curvatus、Leuconostoc mesenteroides subsp. mesenteroides 及び Pediococcus pentosaceus からなる群から選択される乳酸菌により発酵させることを特徴とする海藻発酵組成物。
- グルタミン酸を20mg/dl以上、乳酸を70mg/dl以上含有する、請求項1記載の海藻発酵組成物。
- ワカメ及びコンブからなる群から選択される海藻類をプロテアーゼで処理し、Pediococcus acidilactici、Lactobacillus curvatus、Leuconostoc mesenteroides subsp. mesenteroides 及び Pediococcus pentosaceus からなる群から選択される乳酸菌により発酵させることを特徴とする海藻発酵組成物の製造方法。
- 請求項1又は請求項2に記載の海藻発酵組成物を利用して得られる飲食品。
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KR101937641B1 (ko) | 2017-10-28 | 2019-01-11 | 농업회사법인 (주)다모 | 해조류 발효조미액의 제조방법 |
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JPS6445067A (en) * | 1987-08-12 | 1989-02-17 | Japan Storage Battery Co Ltd | Manufacture of sealed lead-acid battery |
JP2011160761A (ja) * | 2010-02-15 | 2011-08-25 | Kikkoman Corp | 海苔発酵組成物およびその製造方法 |
KR101258739B1 (ko) | 2010-06-25 | 2013-04-29 | 이미경 | 톳을 주원료로 하는 후리가케 조성물 |
DK179900B1 (en) | 2013-06-28 | 2019-09-10 | Fermentationexperts A/S | Compositions comprising fermented seaweed and / or algae |
JP6145353B2 (ja) * | 2013-07-17 | 2017-06-07 | 濱田 奈保子 | Lactobacillus属を用いた発酵ヒダカコンブの血圧上昇抑制剤 |
JP5990209B2 (ja) * | 2014-03-12 | 2016-09-07 | サッポロビール株式会社 | レモン果汁発酵液の製造方法 |
JP7067961B2 (ja) * | 2018-02-28 | 2022-05-16 | 理研ビタミン株式会社 | ビフィズス菌増殖促進剤 |
CN111743150A (zh) * | 2020-07-21 | 2020-10-09 | 集美大学 | 一种海带酵素及其制备方法 |
CN114343143B (zh) * | 2022-01-17 | 2023-06-20 | 厦门元之道生物科技有限公司 | 一种海洋藻类发酵产品及其制备方法 |
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JP3538641B2 (ja) * | 2001-06-25 | 2004-06-14 | 独立行政法人水産総合研究センター | 海藻発酵食品およびその製造方法 |
CN1223283C (zh) * | 2003-08-08 | 2005-10-19 | 李祥麟 | 提高运动员体能的藻菌食品及其制备方法 |
CA2600118A1 (en) * | 2005-03-04 | 2006-09-08 | Suntory Limited | Fermented compositions having immunomodulatory actions |
JP2006320257A (ja) * | 2005-05-19 | 2006-11-30 | Grandhill Osaka:Kk | 海藻発酵エキス、その製造方法およびその用途 |
JP2007267662A (ja) * | 2006-03-31 | 2007-10-18 | Noriyoshi Tsuchiya | 色落ち海苔加工食品素材の製造方法および海苔加工食品素材 |
JP2008086292A (ja) * | 2006-10-04 | 2008-04-17 | Nagatanien:Kk | γ−アミノ酪酸含有食品素材の製造方法 |
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