JP5431732B2 - マイクロ流体フォーマットにおけるアッセイ実装 - Google Patents
マイクロ流体フォーマットにおけるアッセイ実装 Download PDFInfo
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- JP5431732B2 JP5431732B2 JP2008548875A JP2008548875A JP5431732B2 JP 5431732 B2 JP5431732 B2 JP 5431732B2 JP 2008548875 A JP2008548875 A JP 2008548875A JP 2008548875 A JP2008548875 A JP 2008548875A JP 5431732 B2 JP5431732 B2 JP 5431732B2
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- G01N15/10—Investigating individual particles
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- G—PHYSICS
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- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
- G01N2035/00099—Characterised by type of test elements
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- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/44—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from protozoa
- G01N2333/445—Plasmodium
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- G—PHYSICS
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- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
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- G—PHYSICS
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- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70589—CD45
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- G—PHYSICS
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- G01N2800/00—Detection or diagnosis of diseases
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
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- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2575—Volumetric liquid transfer
Description
本出願は、2005年12月22日に出願した米国仮特許出願第60/753,293号の利益を主張する、2006年12月22日に出願した米国特許出願第11/615,884号の一部継続出願である。
{SSALSi,SFALSi}→{drbci,CHCi}
のように細胞直径パラメータおよび細胞ヘモグロビン濃度パラメータにマッピングすることができる。
フローサイトメーター950または960は、AIDS監視/マラリア診断のため遠隔地で使用できる。サイトメーター950または960のような迅速で、配備可能であり、低コストである(計測装置およびアッセイのコスト)計測装置は、市販の大型のフローサイトメトリーシステムに匹敵するか、またはそれ超えるよい結果をもたらしうる。それに加えて、マラリアの場合、これは、感染種を同定することができる低コストで、使いやすいPOCフローサイトメトリースクリーニングの必要性を満たすことができる。携帯型サイトメーター950または960は、開発途上国世界のへんぴな地域でそのようなスクリーニングを行う場合にいくつかの利点を有すると思われる。これらの利点としては、特定の治療を必要とする可能性のある患者の迅速で簡単な識別、関連死亡率および疾病率を有する重度のマラリアへの進行の低減、寄生体抵抗の予防、および多くの人々の良好な患者予後が挙げられる。
本発明は、少なくとも1つの例示的な実施例に関して説明されているけれども、本明細書を読んだ後、多くの変更形態および修正形態が当業者に明らかなものとなるであろう。したがって、付属の請求項が従来技術に関して可能な限り広く、そのようなすべての変更形態および修正形態を含むものと解釈されることが意図されている。
Claims (4)
- ポイントオブケア分析器(10)であって、
携帯型計測装置(12)と、
計測装置(12)内に挿入可能なマイクロ流体カートリッジ(14)とを備え、
前記マイクロ流体カートリッジ(14)は、
透明な窓(474)を有するサイトメトリー流路(472)と、
前記サイトメトリー流路(472)に接続された出口を有する交差領域(470)と、
前記交差領域(470)の第1の入口に接続される第1の流路(462)と、
前記交差領域(470)の第2の入口に接続される第2の流路(464)と、
前記第2の流路(464)に接続されるシース液貯蔵容器と、
前記サイトメトリー流路に接続された廃棄物貯蔵容器とを備え、
前記交差領域(470)は、シース液とともに前記第1の流路(462)から粒子を有する血液試料を、一列縦隊の粒子のコアに流体力学的に集束するためであり、
前記サイトメトリー流路(472)は、サイトメトリー流路(472)の透明な窓(474)を通過するシース液とともに一列縦隊の粒子のコアを廃棄物貯蔵容器へ搬送するためであり、
前記マイクロ流体カートリッジ(14)は前記携帯型計測装置(12)の中に挿入され、前記透明な窓(474)は前記携帯型計測装置内で1以上の光源と1以上の検出器とに整合しており、
前記ポイントオブケア分析器(10)は更に、前記サイトメトリー流路(472)に接続されたヘモグロビンのモジュール(770)を備え、このモジュール(770)は溶血剤堆積領域(771)を有する、
ポイントオブケア分析器(10)。 - マイクロ流体カートリッジ(14)上でマイクロ流体力学ベースのアッセイを行うための方法であって、
全血の試料をマイクロ流体カートリッジ(14)に供給するステップと、
分析器(10)の携帯型計測装置(12)内に配置されているマイクロ流体カートリッジ(14)上でマイクロ流体力学ベースのアッセイを実行するステップとを含み、
前記マイクロ流体力学ベースのアッセイを実行するステップは、さらに、
全血試料を前記マイクロ流体カートリッジ(14)の投入口と第1の流路(462)とに供給するステップと、
前記試料の少なくとも第1の部分を前記第1の流路(462)で溶解するステップと、
交差領域(470)において第2の流路(464)から一列縦隊の粒子のコア内にシース液とともに第1の流路(462)からの試料を流体力学的に集束するステップと、
サイトメトリー流路(472)内のシース液とともに一列縦隊の粒子のコアを携帯型計測装置(12)の光源を介して廃棄物貯蔵容器に搬送するステップと、
マイクロ流体カートリッジ(14)を携帯型計測装置(12)内に挿入するステップであって、マイクロ流体カートリッジ(14)が携帯型計測装置(12)内の1以上の検出器および光源と整合する、前記挿入するステップと、
全血試料の少なくとも第2部分を、溶血剤堆積領域(771)を有するヘモグロビンのモジュール(770)に搬送するステップと、
前記ヘモグロビンのモジュール(770)で、ヘモグロビン吸収技術を使い、前記全血試料の少なくとも第2部分におけるヘモグロビン濃度を決定するステップとを
含む方法。 - ポイントオブケア分析器(10)であって、
免疫アッセイモジュールと、
血液学モジュールを備え、
前記モジュールの少なくとも一部は、前記分析器(10)の計測装置(12)内に挿入可能なカートリッジ(14)に配置され、
前記免疫アッセイモジュールは、第1のマイクロ流体回路(402)を備え、
前記血液学モジュールは、第2のマイクロ流体回路(404)を備え、
前記第1および第2のマイクロ流体回路(402、404)は、前記カートリッジ(14)内に配置され、
前記免疫アッセイモジュールは、
透明な窓(474)を有する第1サイトメトリー流路(472)と、
前記第1サイトメトリー流路(472)に接続された出口を有する第1交差領域(470)と、
前記第1交差領域(470)の第1の入口に接続された第1の流路(462)と、
前記第1交差領域(470)の第2の入口に接続された第2の流路(464)と、
前記第2の流路(464)に接続される第1シース液貯蔵容器と、
前記第1サイトメトリー流路(472)の出口に接続された第1廃棄物貯蔵容器と、
前記第1流路(462)に接続された出口を有する第2交差領域(460)と、
前記第2交差領域(460)に接続される出口を有する第1血液試料貯蔵容器と、
前記第2交差領域(460)に接続される出口を有する溶解液貯蔵容器とを備え、
前記血液学モジュールは、
透明な窓(444)を有する第2サイトメトリー流路(442)と、
前記第2サイトメトリー流路(442)に接続された出口を有する第3交差領域(440)と、
前記第3交差領域の第1の入口に接続された第3の流路(432)と、
前記第3の流路に接続された出口を有する第4交差領域(430)と、
第4の流路(434)に接続される第2シース液貯蔵容器と、
前記第2サイトメトリー流路(442)の出口に接続された第2廃棄物貯蔵容器と、
前記第4交差領域(430)に接続された出口を有する第2血液試料貯蔵容器と、
前記第4交差領域(430)に接続された出口を有する球状化試薬貯蔵容器と、
溶血剤堆積領域(771)を有し、前記第1サイトメトリー流路(472)の出口と連通するヘモグロビンのモジュール(770)とを備える、
ポイントオブケア分析器。 - 血液分析の方法であって、
血液学検査を実行するステップと、
免疫アッセイを実行するステップとを含み、
前記検査およびアッセイは、単一のマイクロ流体カートリッジ(14)により実行され、前記血液学検査は、
全血の試料を用意するステップと、
完全血球算定を実行するステップとを含み、
前記完全血球算定は、
前記試料の第1の部分を溶解し、その結果として白血球を得るステップと、
前記白血球の第1の部分を流体力学的に集束して流路(472)内に押し通し、光を散乱させるステップと、
前記白血球により散乱された光に応じて前記白血球を複数の種類に分画するステップと、
前記試料の第2の部分を流体力学的に集束して流路(442)内に通し、光を散乱させるステップと、
前記赤血球により散乱された光に応じて前記赤血球を計数するステップと、
前記赤血球により散乱された光からヘマトクリットデータを判定するステップと、
ヘモグロビンのモジュール(770)の溶血剤堆積領域(771)と、ヘモグロビンのモジュール(770)の溶血剤堆積領域(771)から少し移動した位置にあるコーナー(772)を通過する前記試料の第3の部分を通過することにより、前記試料の第3の部分のヘモグロビン含有量を光吸収により判定するステップとを含む血液分析の方法。
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US75501405P | 2005-12-29 | 2005-12-29 | |
US60/755,014 | 2005-12-29 | ||
US11/615,884 US8383043B2 (en) | 2004-05-14 | 2006-12-22 | Analyzer system |
US11/615,884 | 2006-12-22 | ||
PCT/US2006/062756 WO2007076549A2 (en) | 2005-12-29 | 2006-12-29 | Assay implementation in a microfluidic format |
US11/618,502 | 2006-12-29 | ||
US11/618,502 US7553453B2 (en) | 2000-06-02 | 2006-12-29 | Assay implementation in a microfluidic format |
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JP2009522556A JP2009522556A (ja) | 2009-06-11 |
JP5431732B2 true JP5431732B2 (ja) | 2014-03-05 |
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JP2008548875A Active JP5431732B2 (ja) | 2005-12-29 | 2006-12-29 | マイクロ流体フォーマットにおけるアッセイ実装 |
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US (1) | US7553453B2 (ja) |
EP (1) | EP1966588B1 (ja) |
JP (1) | JP5431732B2 (ja) |
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WO (1) | WO2007076549A2 (ja) |
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US7553453B2 (en) | 2009-06-30 |
EP1966588B1 (en) | 2018-12-12 |
CN101389947B (zh) | 2013-08-21 |
EP1966588A2 (en) | 2008-09-10 |
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