JP2017537980A - 乾草防腐剤及び乾草の保存方法 - Google Patents
乾草防腐剤及び乾草の保存方法 Download PDFInfo
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- JP2017537980A JP2017537980A JP2017546270A JP2017546270A JP2017537980A JP 2017537980 A JP2017537980 A JP 2017537980A JP 2017546270 A JP2017546270 A JP 2017546270A JP 2017546270 A JP2017546270 A JP 2017546270A JP 2017537980 A JP2017537980 A JP 2017537980A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2442—Chitinase (3.2.1.14)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01014—Chitinase (3.2.1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
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- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Beans For Foods Or Fodder (AREA)
- Treatment Of Sludge (AREA)
- Storage Of Harvested Produce (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
しかしながら、この作業は、植物の継続的な呼吸、機械的損傷による葉の粉砕、及び雨による溶脱に起因する相当な栄養損失をもたらす。この事実の認識により、また予想不能な天候のため、多くの乾草生産者は最適な水分量(20〜30%)よりも高い水分量で梱包し、雨による被害と葉の機械的損失のリスクを最小限にしている。しかしながら、この実務は酵母、カビ、及び時には細菌の活動による損害、熱の発生、及び給与する際の乾草の栄養価の低下をもたらしている。
1PA=ピキア・アノマラ;CE=キチナーゼ活性保有酵素;BPA=プロピオン酸;CON=コントロール
2TB=寒天培地で成長する非選好性細菌;LAB=MRS培地で成長する、乳酸桿菌と推定される細菌;OM=有機物;NH3=アンモニア性窒素;TN=総窒素;LA=乳酸;ADF=酸性デタージェント線維;ADIN=酸性洗剤不溶酵素
1PA=ピキア・アノマラ;CE=キチナーゼ活性保有酵素;BPA=プロピオン酸;CON=コントロール
2TB=寒天培地で成長する非選好性細菌;LAB=MRS培地で成長する、乳酸桿菌と推定される細菌;OM=有機物;NH3=アンモニア性窒素;TN=総窒素;LA=乳酸;ADF=酸性デタージェント線維;ADIN=酸性洗剤不溶酵素
1PA=ピキア・アノマラ;CE=キチナーゼ活性保有酵素;BPA=プロピオン酸;CON=コントロール
PA=ピキア・アノマラ;CE=キチナーゼ活性保有酵素;BPA=プロピオン酸;CON=コントロール
色/におい Maryland Cooperative Extension Fact Sheet#644 “Evaluating Hay Quality”による。
(http://www.extension.umd.edu/publications/pdfs/fs644.pdf)
色
明るい緑色の乾草は高いポイントとする(15〜20)。
ゴールデンイエロー〜黄色の乾草は5〜15ポイントとする。
茶褐色〜黒色の乾草は0〜5ポイントとする。
におい
新しく刈った乾草のにおいは高いポイントとする(15〜20ポイント)。
カビ臭い又は他の悪臭の乾草は5〜15ポイントとする。
カビが生えた又は異常なほこりっぽい乾草は非常に低いポイントとする(0〜5ポイント)。
グレード
グレードは審査員による全体評価に基づき、非常に劣る(very poor)、劣る(poor)、平均(average)、良好(good)、非常に良好(very good)、極めて良好(excellent)で評価する。
ペディオコッカス・ペントサセウスと組み合わせたキチナーゼ活性保有酵素の組み合わせは、乾草1トン当たり、1Lの水に1011CFU、及び1.5gを使用した。ピキア・アノマラ単独については乾草1トン当たり、1Lに1011CFU使用した。ペディオコッカス・ペントサセウス単独については乾草1トン当たり、1Lに1011CFU使用した。キチナーゼ活性保有酵素単独については、乾草1トン当たり1.5g(1Lの水に懸濁)の割合で使用した。プロピオン酸製品は
68%(vol/vol)プロピオン酸からなり、2.72L/トンの量でポジティブコントロールとして使用した。ネガティブコントロールは水であり、乾草1トン当たり1Lの割合で使用した。約800kgの5つの大きな丸いベールを同じ日に各処理に対して実施例1に従って作成した。
Claims (24)
- 乾草防腐及び減熱に有効な量の少なくとも1種類のキチナーゼ活性保有酵素を、単独で又は乾草防腐及び減熱に有効な量のピキア属の酵母又はペディオコッカス属の細菌と組み合わせて含む、乾草防腐剤。
- 前記少なくとも1種類のキチナーゼ活性保有酵素が被処理乾草1トン当たり約6U〜300Uの範囲のキチナーゼ活性を保有する、請求項1に記載の乾草防腐剤。
- 前記ピキア属の酵母がピキア・アノマラ種に由来する、請求項1又は2に記載の乾草防腐剤。
- 前記ピキア属の酵母が、受託番号DBVPG 3003のピキア・アノマラ株の識別特性の全てを備えたピキア・アノマラ種に由来する、請求項1〜3のいずれか一項に記載の乾草防腐剤。
- 前記ペディオコッカス属の細菌がペディオコッカス・ペントサセウス種に由来する、請求項1又は2に記載の乾草防腐剤。
- 前記ペディオコッカス属の細菌が、受託番号NCIMB 12674のペディオコッカス・ペントサセウスBTC328株又は受託番号NCIMB 12675のペディオコッカス・ペントサセウスBTC401株の識別特性の全てを備えたペディオコッカス・ペントサセウス種に由来する、請求項1、2又は5に記載の乾草防腐剤。
- 前記少なくとも1種類のキチナーゼ活性保有酵素が乾草防腐及び減熱に有効な量のピキア・アノマラ種と組み合わせられる、請求項1に記載の乾草防腐剤。
- 前記少なくとも1種類のキチナーゼ活性保有酵素が乾草防腐及び減熱に有効な量のペディオコッカス・ペントサセウス種と組み合わせられる、請求項1に記載の乾草防腐剤。
- ペクチンリアーゼ活性、グルカナーゼ活性又はそれらの混合活性を保有する少なくとも1種類の酵素をさらに含む、請求項1〜8のいずれか一項に記載の乾草防腐剤。
- 高水分乾草における熱損傷を防止及び/又は低減するため、並びにそれを防腐するために乾草を処理する方法であって、乾草に、乾草防腐及び減熱に有効な量の少なくとも1種類のキチナーゼ活性保有酵素を単独で又は乾草防腐及び減熱に有効な量のピキア属の酵母又はペディオコッカス属の細菌と組み合わせて含む乾草防腐剤を添加することを含む方法。
- 前記少なくとも1種類のキチナーゼ活性保有酵素が被処理乾草1トン当たり約6U〜300Uの範囲のキチナーゼ活性を保有する、請求項10に記載の方法。
- 前記ピキア属の酵母がピキア・アノマラ種である、請求項10又は11に記載の方法。
- 前記ピキア属の酵母が受託番号DBVPG 3003のピキア・アノマラ株の識別特性の全てを備えたピキア・アノマラ種である、請求項10〜12のいずれか一項に記載の方法。
- 前記ペディオコッカス属の酵母がペディオコッカス・ペントサセウス種である、請求項10又は11に記載の方法。
- 前記ペディオコッカス属の酵母が受託番号NCIMB 12674のペディオコッカス・ペントサセウスBTC328株又は受託番号NCIMB 12675のペディオコッカス・ペントサセウスBTC401株の全ての識別特性を備えたペディオコッカス・ペントサセウス種である、請求項10、11又は14に記載の方法。
- ペクチンリアーゼ活性、グルカナーゼ活性又はそれらの混合活性を保有する少なくとも1種類の酵素をさらに含む、請求項10〜15のいずれか一項に記載の方法。
- 請求項1〜9のいずれか一項に規定される乾草防腐剤を含む高水分乾草。
- 乾草防腐及び減熱に有効な量のピキア属の酵母を含む乾草防腐剤。
- 前記ピキア属の酵母がピキア・アノマラ種に由来する、請求項18に記載の乾草防腐剤。
- 前記ピキア属の酵母が受託番号DBVPG 3003のピキア・アノマラ株の全ての識別特性を備えたピキア・アノマラ種に由来する、請求項18又は19に記載の乾草防腐剤。
- 請求項18〜20のいずれか一項に規定される乾草防腐剤を含む高水分乾草。
- 高水分乾草における熱損傷を防止及び/又は低減するため、並びにそれを防腐するために乾草を処理する方法であって、乾草に、乾草防腐及び減熱に有効な量のピキア属の酵母を含む乾草防腐剤を添加することを含む方法。
- 前記ピキア属の酵母がピキア・アノマラ種である、請求項22に記載の方法。
- 前記ピキア属の酵母が受託番号DBVPG 3003のピキア・アノマラ株の全ての識別特性を備えたピキア・アノマラ種である、請求項22又は23に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14194567.5A EP3023490A1 (en) | 2014-11-24 | 2014-11-24 | Hay preservative and methods for preservation of hay |
EP14194567.5 | 2014-11-24 | ||
PCT/IB2015/059079 WO2016083996A1 (en) | 2014-11-24 | 2015-11-24 | Hay preservative and methods for preservation of hay |
Related Child Applications (1)
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JP2020174369A Division JP2021035952A (ja) | 2014-11-24 | 2020-10-16 | 乾草防腐剤及び乾草の保存方法 |
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JP2017537980A true JP2017537980A (ja) | 2017-12-21 |
JP2017537980A5 JP2017537980A5 (ja) | 2020-04-23 |
JPWO2016083996A5 JPWO2016083996A5 (ja) | 2023-01-06 |
JP7233839B2 JP7233839B2 (ja) | 2023-03-07 |
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JP2017546270A Active JP7233839B2 (ja) | 2014-11-24 | 2015-11-24 | 乾草防腐剤及び乾草の保存方法 |
JP2020174369A Withdrawn JP2021035952A (ja) | 2014-11-24 | 2020-10-16 | 乾草防腐剤及び乾草の保存方法 |
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JP2020174369A Withdrawn JP2021035952A (ja) | 2014-11-24 | 2020-10-16 | 乾草防腐剤及び乾草の保存方法 |
Country Status (19)
Country | Link |
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US (1) | US11903397B2 (ja) |
EP (2) | EP3023490A1 (ja) |
JP (2) | JP7233839B2 (ja) |
CN (1) | CN106998750B (ja) |
AR (2) | AR103206A1 (ja) |
AU (1) | AU2015352045B2 (ja) |
BR (1) | BR112017010766B1 (ja) |
CA (1) | CA2967206C (ja) |
CL (1) | CL2017001228A1 (ja) |
CO (1) | CO2017005171A2 (ja) |
DK (1) | DK3224345T3 (ja) |
EA (1) | EA034723B1 (ja) |
ES (1) | ES2864217T3 (ja) |
HU (1) | HUE053811T2 (ja) |
MX (1) | MX2017006721A (ja) |
NZ (1) | NZ731618A (ja) |
PL (1) | PL3224345T3 (ja) |
PT (1) | PT3224345T (ja) |
WO (1) | WO2016083996A1 (ja) |
Cited By (1)
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JP2021035952A (ja) * | 2014-11-24 | 2021-03-04 | ダンスター フェルマン アーゲー | 乾草防腐剤及び乾草の保存方法 |
Families Citing this family (1)
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CN114128792A (zh) * | 2021-12-15 | 2022-03-04 | 内蒙古自治区农牧业科学院 | 一种利用植物精油熏蒸抑制高水分苜蓿贮藏发霉的方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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JPS4856819A (ja) * | 1971-11-11 | 1973-08-09 | ||
JPS4947152A (ja) * | 1972-09-19 | 1974-05-07 | ||
JPH1042861A (ja) * | 1996-08-02 | 1998-02-17 | Norin Suisansyo Sochi Shikenjo | 乳酸非資化性キラー酵母 |
JPH11196860A (ja) * | 1998-01-08 | 1999-07-27 | Rikagaku Kenkyusho | サイレージ調製用乳酸菌 |
JP2000109405A (ja) * | 1998-08-04 | 2000-04-18 | Sanin Kensetsu Kogyo Kk | キチナ―ゼを用いた植物病原菌防除剤 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4820531A (en) * | 1987-10-22 | 1989-04-11 | Pioneer Hi-Bred International | Bacterial treatment to preserve hay quality by addition of microorganisms of the genus bacillus |
US5371011A (en) * | 1989-07-10 | 1994-12-06 | Zeneca Corp. | Mold control in forage |
EP0408220B1 (en) * | 1989-07-10 | 1993-09-08 | ZENECA Corp. | Mould control in forage |
GB9027303D0 (en) | 1990-12-17 | 1991-02-06 | Enzymatix Ltd | Enzyme formulation |
EP0906020B1 (en) | 1996-06-07 | 2002-02-06 | Dsm N.V. | Use of a fungal composition for crop protection |
SE513894C2 (sv) * | 1998-03-12 | 2000-11-20 | Bioagri Ab | Förfarande för lufttät lagring av fuktigt spannmål i närvaro av ett biokontrollmedel |
JP4856819B2 (ja) | 2001-06-28 | 2012-01-18 | 東芝機械株式会社 | 破砕機 |
US7087810B2 (en) * | 2001-11-07 | 2006-08-08 | Muller Mathis L | Isolated nucleic acids encoding proteins with chitinase activity and uses thereof |
CN104146151A (zh) | 2014-09-01 | 2014-11-19 | 陕西省西安植物园 | 一种垂穗披碱草青贮饲料的生产方法 |
EP3023490A1 (en) * | 2014-11-24 | 2016-05-25 | Danstar Ferment AG | Hay preservative and methods for preservation of hay |
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2014
- 2014-11-24 EP EP14194567.5A patent/EP3023490A1/en not_active Withdrawn
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2015
- 2015-11-24 US US15/528,998 patent/US11903397B2/en active Active
- 2015-11-24 HU HUE15804608A patent/HUE053811T2/hu unknown
- 2015-11-24 PT PT158046086T patent/PT3224345T/pt unknown
- 2015-11-24 BR BR112017010766-0A patent/BR112017010766B1/pt active IP Right Grant
- 2015-11-24 AR ARP150103839A patent/AR103206A1/es active IP Right Grant
- 2015-11-24 JP JP2017546270A patent/JP7233839B2/ja active Active
- 2015-11-24 EA EA201791141A patent/EA034723B1/ru not_active IP Right Cessation
- 2015-11-24 WO PCT/IB2015/059079 patent/WO2016083996A1/en active Application Filing
- 2015-11-24 AU AU2015352045A patent/AU2015352045B2/en active Active
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Cited By (1)
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JP2021035952A (ja) * | 2014-11-24 | 2021-03-04 | ダンスター フェルマン アーゲー | 乾草防腐剤及び乾草の保存方法 |
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BR112017010766B1 (pt) | 2022-04-19 |
CA2967206C (en) | 2022-08-16 |
PT3224345T (pt) | 2021-03-12 |
US11903397B2 (en) | 2024-02-20 |
CO2017005171A2 (es) | 2017-10-31 |
CA2967206A1 (en) | 2016-06-02 |
CN106998750B (zh) | 2022-04-15 |
AU2015352045A1 (en) | 2017-05-25 |
MX2017006721A (es) | 2018-03-16 |
WO2016083996A1 (en) | 2016-06-02 |
AU2015352045B2 (en) | 2022-01-27 |
EP3224345A1 (en) | 2017-10-04 |
BR112017010766A2 (pt) | 2018-01-09 |
AR103206A1 (es) | 2017-04-26 |
PL3224345T3 (pl) | 2021-07-12 |
JP2021035952A (ja) | 2021-03-04 |
EA034723B1 (ru) | 2020-03-12 |
HUE053811T2 (hu) | 2021-07-28 |
CL2017001228A1 (es) | 2018-05-11 |
DK3224345T3 (da) | 2021-03-15 |
EP3224345B1 (en) | 2020-12-23 |
AR125341A2 (es) | 2023-07-05 |
NZ731618A (en) | 2019-09-27 |
EP3023490A1 (en) | 2016-05-25 |
CN106998750A (zh) | 2017-08-01 |
ES2864217T3 (es) | 2021-10-13 |
EA201791141A1 (ru) | 2017-10-31 |
JP7233839B2 (ja) | 2023-03-07 |
US20170311625A1 (en) | 2017-11-02 |
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