JP2015507930A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2015507930A5 JP2015507930A5 JP2014557769A JP2014557769A JP2015507930A5 JP 2015507930 A5 JP2015507930 A5 JP 2015507930A5 JP 2014557769 A JP2014557769 A JP 2014557769A JP 2014557769 A JP2014557769 A JP 2014557769A JP 2015507930 A5 JP2015507930 A5 JP 2015507930A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- atn
- thio
- amino
- dna polymerase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 11
- 108020004707 nucleic acids Proteins 0.000 claims 11
- IQFYYKKMVGJFEH-XLPZGREQSA-N DEOXYTHYMIDINE Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims 8
- 101700011961 DPOM Proteins 0.000 claims 6
- 101710029649 MDV043 Proteins 0.000 claims 6
- 101700061424 POLB Proteins 0.000 claims 6
- 101700054624 RF1 Proteins 0.000 claims 6
- 230000003321 amplification Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- YKBGVTZYEHREMT-KVQBGUIXSA-N Deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 claims 4
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N dCyd Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 3
- NOLHIMIFXOBLFF-KVQBGUIXSA-N (2R,3S,5R)-5-(2,6-diaminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol Chemical compound C12=NC(N)=NC(N)=C2N=CN1[C@H]1C[C@H](O)[C@@H](CO)O1 NOLHIMIFXOBLFF-KVQBGUIXSA-N 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 239000001226 triphosphate Substances 0.000 claims 2
- 235000011178 triphosphate Nutrition 0.000 claims 2
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 claims 2
- PISWNSOQFZRVJK-XLPZGREQSA-N 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methyl-2-sulfanylidenepyrimidin-4-one Chemical compound S=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 PISWNSOQFZRVJK-XLPZGREQSA-N 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000005549 deoxyribonucleoside Substances 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
Claims (8)
- 核酸の増幅方法であって、
ａ）核酸鋳型を用意するステップと、
ｂ）ＤＮＡポリメラーゼと、デオキシリボヌクレオシド三リン酸と、３’末端及び５’末端を有するプライマーとを含む反応溶液に核酸鋳型を接触させるステップであって、プライマーが、２−アミノ−デオキシアデノシン（２−アミノ−ｄＡ）及び２−チオ−デオキシチミジン（２−チオ−ｄＴ）を含んでいて、（＋Ｎ）（＋Ｎ）（ａｔＮ）（ａｔＮ）（ａｔＮ）＊Ｎの一般構造（式中、ヘキサマーの５’末端は（＋Ｎ）、３’末端は＊Ｎであり、「Ｎ」はデオキシアデノシン（ｄＡ）、デオキシシチジン（ｄＣ）、デオキシグアノシン（ｄＧ）又はデオキシチミジン（ｄＴ）を表し、「＋」はヌクレオチド塩基に先行するロックド核酸（ＬＮＡ）塩基を示し、（ａｔＮ）は２−アミノ−ｄＡ、ｄＣ、ｄＧ及び２−チオ−ｄＴのランダム混合物を表し、「＊」はホスホロチオエート結合を表す。）を有するヘキサマーである、ステップと、
ｃ）核酸鋳型を増幅するステップと
を含む方法。 - 核酸鋳型の増幅が等温条件下で行われる、請求項１記載の方法。
- 核酸鋳型の増幅が高いストリンジェンシー条件下で行われる、請求項１記載の方法。
- ＤＮＡポリメラーゼがｐｈｉ２９ＤＮＡポリメラーゼである、請求項１記載の方法。
- 核酸鋳型の増幅がローリングサークル増幅法（ＲＣＡ）又は多重置換増幅法（ＭＤＡ）からなる、請求項１記載の方法。
- 当該方法により、痕跡量の核酸の増幅が可能である、請求項１記載の方法。
- 核酸を増幅するためのキットであって、
（ａ）ＤＮＡポリメラーゼと、
（ｂ）デオキシリボヌクレオシド三リン酸と、
（ｃ）３’末端及び５’末端を有するプライマーであって、２−アミノ−デオキシアデノシン（２−アミノ−ｄＡ）及び２−チオ−デオキシチミジン（２−チオ−ｄＴ）を含んでいて、（＋Ｎ）（＋Ｎ）（ａｔＮ）（ａｔＮ）（ａｔＮ）＊Ｎの一般構造（式中、ヘキサマーの５’末端は（＋Ｎ）、３’末端は＊Ｎであり、「Ｎ」はデオキシアデノシン（ｄＡ）、デオキシシチジン（ｄＣ）、デオキシグアノシン（ｄＧ）又はデオキシチミジン（ｄＴ）を表し、「＋」はヌクレオチド塩基に先行するロックド核酸（ＬＮＡ）塩基を示し、（ａｔＮ）は２−アミノ−ｄＡ、ｄＣ、ｄＧ及び２−チオ−ｄＴのランダム混合物を表し、「＊」はホスホロチオエート結合を表す。）を有するヘキサマーであるプライマーと
を含むキット。 - ＤＮＡポリメラーゼがｐｈｉ２９ＤＮＡポリメラーゼである、請求項７記載のキット。
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261599119P | 2012-02-15 | 2012-02-15 | |
| US61/599,119 | 2012-02-15 | ||
| US13/446,474 | 2012-04-13 | ||
| US13/446,474 US9353393B2 (en) | 2012-02-15 | 2012-04-13 | Methods and kits for reducing non-specific nucleic acid amplification |
| PCT/US2013/026136 WO2013123187A1 (en) | 2012-02-15 | 2013-02-14 | Methods and kits for reducing non-specific nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015507930A JP2015507930A (ja) | 2015-03-16 |
| JP2015507930A5 true JP2015507930A5 (ja) | 2017-08-03 |
| JP6225123B2 JP6225123B2 (ja) | 2017-11-01 |
Family
ID=48945882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014557769A Active JP6225123B2 (ja) | 2012-02-15 | 2013-02-14 | 非特異的核酸増幅を低減するための方法及びキット |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US9353393B2 (ja) |
| EP (1) | EP2814976B1 (ja) |
| JP (1) | JP6225123B2 (ja) |
| CN (1) | CN104093850B (ja) |
| CA (1) | CA2864676C (ja) |
| WO (1) | WO2013123187A1 (ja) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2917400T3 (es) | 2012-07-26 | 2022-07-08 | Illumina Inc | Composiciones y métodos para la amplificación de ácidos nucleicos |
| US9938568B2 (en) | 2013-07-26 | 2018-04-10 | General Electric Company | Ligase-assisted nucleic acid circularization and amplification |
| US9587263B2 (en) * | 2014-03-26 | 2017-03-07 | General Electric Company | Isothermal amplification under low salt condition |
| US10683534B2 (en) * | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| EP3201350B1 (en) | 2014-09-30 | 2020-12-02 | Global Life Sciences Solutions USA LLC | Method for nucleic acid analysis directly from an unpurified biological sample |
| ES2963004T3 (es) | 2015-09-09 | 2024-03-22 | Drawbridge Health Inc | Dispositivos para la recopilación, estabilización y conservación de muestras |
| US10077459B2 (en) | 2016-05-04 | 2018-09-18 | General Electric Company | Cell-free protein expression using rolling circle amplification product |
| WO2018209092A1 (en) * | 2017-05-10 | 2018-11-15 | Board Of Regents, The University Of Texas System | Methods and devices related to amplifying nucleic acid at a variety of temperatures |
| US11001868B2 (en) * | 2017-08-11 | 2021-05-11 | Global Life Sciences Solutions Operations UK Ltd | Cell-free protein expression using double-stranded concatameric DNA |
| CN111334562A (zh) * | 2018-12-19 | 2020-06-26 | 合肥铼科生物科技有限公司 | 一种含有修饰引物的核酸扩增方法和试剂盒 |
| CN110295241A (zh) * | 2019-07-11 | 2019-10-01 | 深圳易致生物科技有限公司 | 用于检测***的引物组和包含该引物组的试剂盒 |
| FR3110602A1 (fr) | 2020-05-20 | 2021-11-26 | Anova-Plus | Methode de detection sur le terrain de la presence et de la mutliresistance d’un bio-agresseur, notamment dezymo-septoria tritici,dans des culturescerealieres |
| CN112899346A (zh) * | 2021-01-05 | 2021-06-04 | 南京普济生物有限公司 | 一种降低pcr非特异性扩增的核苷酸及其设计方法与应用 |
| FR3129407A1 (fr) | 2021-11-25 | 2023-05-26 | Anova-Plus | Methode de detection de la presence d’un pathogene et determination de ses caracteristiques en sante humaine et animale |
| CN114277103A (zh) * | 2022-01-21 | 2022-04-05 | 杭州飞时达生物科技有限公司 | 一种基于六聚体随机引物高效滚环扩增方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040086880A1 (en) * | 1999-07-20 | 2004-05-06 | Sampson Jeffrey R | Method of producing nucleic acid molecules with reduced secondary structure |
| AU2001216942A1 (en) | 2000-11-24 | 2002-06-03 | Asper Ou | Method of optimising the sequences of synthetic nucleic acids |
| US7993839B2 (en) * | 2001-01-19 | 2011-08-09 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
| US20020197618A1 (en) * | 2001-01-20 | 2002-12-26 | Sampson Jeffrey R. | Synthesis and amplification of unstructured nucleic acids for rapid sequencing |
| US6617137B2 (en) | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| US7176002B2 (en) | 2002-05-16 | 2007-02-13 | Applera Corporation | Universal-tagged oligonucleotide primers and methods of use |
| EP1426448A1 (en) | 2002-12-03 | 2004-06-09 | bioMérieux BV | Method for lowering the effects of sequence variations in a diagnostic hybridization assay, probe for use in the assay and assay |
| US8871469B1 (en) | 2004-11-13 | 2014-10-28 | Steven Albert Benner | Self-avoiding molecular recognition systems in DNA priming |
| WO2006119066A2 (en) | 2005-04-29 | 2006-11-09 | The J. Craig Venter Institute | Amplification and cloning of single dna molecules using rolling circle amplification |
| JP5558811B2 (ja) | 2006-06-01 | 2014-07-23 | トリリンク バイオテクノロジーズ | 核酸増幅のための化学的に修飾されたオリゴヌクレオチドプライマー |
| AU2008272421B2 (en) | 2007-07-03 | 2014-09-04 | Genaphora Ltd. | Chimeric primers for improved nucleic acid amplification reactions |
| US20090191553A1 (en) | 2007-10-01 | 2009-07-30 | Applied Biosystems Inc. | Chase Ligation Sequencing |
| EP2488664A4 (en) | 2009-10-13 | 2014-04-09 | Syntezza Molecular Detection Israel Ltd | METHOD AND COMPOSITIONS FOR REINFORCING TARGET SEQUENCES FROM NUCLEIC ACID SAMPLES |
| US20110195457A1 (en) | 2010-02-09 | 2011-08-11 | General Electric Company | Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof |
-
2012
- 2012-04-13 US US13/446,474 patent/US9353393B2/en active Active
-
2013
- 2013-02-14 CA CA2864676A patent/CA2864676C/en active Active
- 2013-02-14 CN CN201380009386.6A patent/CN104093850B/zh active Active
- 2013-02-14 WO PCT/US2013/026136 patent/WO2013123187A1/en active Application Filing
- 2013-02-14 EP EP13749671.7A patent/EP2814976B1/en active Active
- 2013-02-14 JP JP2014557769A patent/JP6225123B2/ja active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2015507930A5 (ja) | ||
| ES2610461T3 (es) | Método de detección de nucleótidos únicos | |
| WO2011142861A3 (en) | Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers | |
| JP2016195614A5 (ja) | ||
| JP2010142229A5 (ja) | ||
| JP2015156872A5 (ja) | ||
| WO2015081229A3 (en) | Selective amplification of nucleic acid sequences | |
| ES2559108T3 (es) | Método de detección de nucleótidos simples | |
| JP2017508474A5 (ja) | ||
| JP2016526876A5 (ja) | ||
| JP2010004884A5 (ja) | ||
| WO2014004852A3 (en) | Kit for isothermal dna amplification starting from an rna template | |
| WO2015089511A3 (en) | Modified nucleic acid molecules and uses thereof | |
| JP2014513534A5 (ja) | ||
| WO2009102896A3 (en) | Isothermal nucleic acid amplification methods and compositions | |
| WO2014145296A3 (en) | Nuclei acid amplification | |
| WO2010117457A3 (en) | System comprising dual-sided thermal cycler and emulsion pcr in pouch | |
| JP2016540517A5 (ja) | ||
| JP2015533507A5 (ja) | ||
| JP2017530715A5 (ja) | ||
| JP2016507242A5 (ja) | ||
| WO2011106368A3 (en) | Amplification methods to minimise sequence specific bias | |
| GB0712882D0 (en) | Nucleic acid amplification | |
| WO2015148218A3 (en) | Isothermal amplification under low salt condition | |
| WO2012177695A3 (en) | Hybrid polymerases having the ability to produce long amplicons |