FI104493B - Syrafosfatasgen av Pichia pastoris-jäst - Google Patents
Syrafosfatasgen av Pichia pastoris-jäst Download PDFInfo
- Publication number
- FI104493B FI104493B FI915893A FI915893A FI104493B FI 104493 B FI104493 B FI 104493B FI 915893 A FI915893 A FI 915893A FI 915893 A FI915893 A FI 915893A FI 104493 B FI104493 B FI 104493B
- Authority
- FI
- Finland
- Prior art keywords
- seq
- sequence
- gene
- dna
- pichia pastoris
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/938—Pichia
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (15)
1. Isolerat DNA-fragment, kännetecknat av att det innefattar en syrafosfatasgen av Pichia pastoria -jäst, vars nukleotidsek-vens är SEQ ID NO 1.
2. Isolerat DNA-fragment, kannetecknat av att det innefattar en 5'-regleringsregion av syrafosfatasen av Pichia pastori s -jästen, vars nukleotidsekvens är SEQ ID NO 2.
3. Isolerat DNA-fragment, kännetecknat av att det innefattar en signalsekvens av syrafosfatasen av Pichia pastoria-jästen, vars nukleotidsekvens är SEQ ID NO 3.
4. DNA-fragment enligt patentkrav 2 eller 3, kännetecknat av att nämnda DNA-fragment är funktionellt kopplat tili en hetero-logisk DNA-sekvens som kodar minst för invertas eller vävnads-plasminogenaktivator (tPA).
5. Förfarande för sekretering av minst invertas eller tPA ur en Pichia pastoria -cell, kännetecknat av att denna sekretering styrs av närvaron av signalsekvensen (SEQ ID NO 3) som härrör frän syraf osfatasgenen av Pichia pastori s -jästen.
6. Isolerat DNA-fragment, kännetecknat av att det innefattar ·.· en strukturgen av syrafosfatasen av Pichia pastoria -jästen, vars nukleotidsekvens är SEQ ID NO 4.
7. Isolerat DNA-fragment, kännetecknat av att det innefattar en 3'-transkriptionsterminalsekvens av syrafosfatasen av Pichia pastori h -jästen, vars nukleotidsekvens är SEQ ID NO 5.
8. Plasmid pLP24, säsom har beskrivits i exempel I.
9. Plasmid pT37, säsom har beskrivits i exempel IX, 2.
10. Förfarande för aktivering av regleringsregionen av syrafosfatasen som härrör frän syrafosfatasgenen av Pichia pastnris -jästen för att underlätta expressionen av den heterologiska m 104493 DNA-sekvensen som kodar minst för en polypeptid, kannetecknat av att Pichia pastoris, som är transformerad med 5'-reglerings-regionen (SEQ ID NO 2) av syrafosfatasen funktionellt kopplat tili den heterologiska sekvensen, sätts i beröring med ett sädant underlag, vars fosfathalt ästadkommer expressionen.
11. Förfarande enligt patentkrav 10, kännetecknat av att nämnda heterologiska DNA även innefattar en signalsekvens som är funktionellt kopplad tili den, speciellt signalsekvensen (SEQ ID NO 3) av syrafosfatasen av Pichia pastori s -jästen.
12. Isolerat DNA-fragment, kännetecknat av att det innefattar signalsekvensen (SEQ ID NO 3) av syrafosfatasen av Pichia pastori s -jästen funktionellt kopplad tili 5'-regleringsregionen, speciellt tili 5regleringsregionen (SEQ ID NO 2) av syrafosfatasen av Pichia pastnris -jästen eller tili 5'-regleringsregionen av alkoholoxidasen (AOX1) av Pichia pastoria -jästen.
13. DNA-fragment enligt patentkrav 12, kännetecknat av att nämnda DNA-fragment är funktionellt kopplat tili den heterologiska DNA-sekvensen som kodar minst för invertas och tPA.
14. Förfarande för att styra integreringen tili Pichia pastoria PHOT -locus med hjälp av en integrerande vektor som innefattar 5'- och 3'-sekvenser som omramar Pichia pastori s PHQ1 -genen.
15. Förfarande för identifiering av Pichia pastori s - trans-formanter som innefattar tili PHOl-locuset integrerade expres-sionsvektorer, kännetecknat av att transformerad Pichia pastoria överförs tili indikatorkärl, vars fosfathalt är smä, och .* kolonierna läts växa över natten, efter vilket ur kolonierna isoleras de kolonier som är vita tili färgen.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US62753990 | 1990-12-14 | ||
US07/627,539 US5268273A (en) | 1990-12-14 | 1990-12-14 | Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same |
Publications (3)
Publication Number | Publication Date |
---|---|
FI915893A0 FI915893A0 (fi) | 1991-12-13 |
FI915893A FI915893A (fi) | 1992-06-15 |
FI104493B true FI104493B (sv) | 2000-02-15 |
Family
ID=24515081
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI915893A FI104493B (sv) | 1990-12-14 | 1991-12-13 | Syrafosfatasgen av Pichia pastoris-jäst |
FI992503A FI113474B (sv) | 1990-12-14 | 1999-11-24 | DNA-fragment av syrafosfatasgen av jästen Pichia pastoris |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI992503A FI113474B (sv) | 1990-12-14 | 1999-11-24 | DNA-fragment av syrafosfatasgen av jästen Pichia pastoris |
Country Status (22)
Country | Link |
---|---|
US (1) | US5268273A (sv) |
EP (1) | EP0495208B1 (sv) |
JP (1) | JP3437850B2 (sv) |
KR (1) | KR0181179B1 (sv) |
CN (1) | CN1062169A (sv) |
AR (1) | AR245218A1 (sv) |
AT (1) | ATE208822T1 (sv) |
AU (1) | AU637734B2 (sv) |
CA (1) | CA2055542C (sv) |
CS (1) | CS378891A3 (sv) |
DE (1) | DE69132812T2 (sv) |
FI (2) | FI104493B (sv) |
HU (1) | HU913926D0 (sv) |
IE (1) | IE914354A1 (sv) |
IL (1) | IL100363A0 (sv) |
MX (1) | MX9102546A (sv) |
NO (1) | NO314151B1 (sv) |
PL (1) | PL292765A1 (sv) |
PT (1) | PT99802A (sv) |
TW (1) | TW201330B (sv) |
YU (1) | YU193591A (sv) |
ZA (1) | ZA919776B (sv) |
Families Citing this family (51)
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US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
GB9307371D0 (en) * | 1993-04-08 | 1993-06-02 | Walls Alan J | Fusion proteins |
US5521086A (en) * | 1993-09-16 | 1996-05-28 | Cephalon, Inc. | Secretion sequence for the production of a heterologous protein in yeast |
IT1266740B1 (it) * | 1994-07-01 | 1997-01-14 | Maria Paola Landini | Materiale proteico ricombinante legante anticorpi contro il citomegalovirus umano, reagenti diagnostici derivati da tale |
CA2202351A1 (en) * | 1994-10-18 | 1996-04-25 | George Phillip Vlasuk | Nematode-extracted serine protease inhibitors and anticoagulant proteins |
US5863894A (en) * | 1995-06-05 | 1999-01-26 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
US5945275A (en) * | 1994-10-18 | 1999-08-31 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
US5866542A (en) * | 1994-10-18 | 1999-02-02 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
US5872098A (en) * | 1995-06-05 | 1999-02-16 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
US5866543A (en) * | 1995-06-05 | 1999-02-02 | Corvas International, Inc. | Nematode-extracted anticoagulant protein |
PL183855B1 (pl) * | 1994-10-18 | 2002-07-31 | Corvas International | Białko, cząsteczka cDNA, oligonukleotyd, kompozycja farmaceutyczna i środek farmaceutyczny do hamowania krzepnięcia krwi |
AU2115897A (en) * | 1996-02-01 | 1997-08-22 | North American Vaccine, Inc. | Expression of group b neisseria meningitidis outer membrane (mb3) protein from yeast and vaccines |
MX9605082A (es) | 1996-10-24 | 1998-04-30 | Univ Autonoma De Nuevo Leon | Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano. |
EP1003889A1 (en) * | 1997-08-05 | 2000-05-31 | Chiron Corporation | NOVEL $i(PICHIA PASTORIS) GENE SEQUENCES AND METHODS FOR THEIR USE |
US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
AU2614500A (en) | 1999-01-14 | 2000-08-01 | Novozymes Biotech, Inc. | Polypeptides having acid phosphatase activity and nucleic acids encoding same |
AU4056700A (en) | 1999-03-31 | 2000-10-16 | Cornell Research Foundation Inc. | Phosphatases with improved phytase activity |
US6841370B1 (en) | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
CN1320699A (zh) * | 2000-04-27 | 2001-11-07 | 上海博德基因开发有限公司 | 一种新的多肽——人磷脂酶9和编码这种多肽的多核苷酸 |
CN1324948A (zh) * | 2000-05-24 | 2001-12-05 | 上海博德基因开发有限公司 | 一种新的多肽——酸性磷酸酶14和编码这种多肽的多核苷酸 |
AU2002210126A1 (en) | 2000-05-25 | 2001-12-03 | Chiron Corporation | Iron regulation of the ppsec10 transcriptional regulatory region of pichia pastoris and methods of use |
CN1329020A (zh) * | 2000-06-21 | 2002-01-02 | 上海博德基因开发有限公司 | 一种新的多肽——酸性磷酸酶家族蛋白11和编码这种多肽的多核苷酸 |
US7009045B2 (en) * | 2000-07-14 | 2006-03-07 | Archer-Daniels-Midland Company | Transformation systems for flavinogenic yeast |
WO2003037102A2 (en) * | 2001-10-31 | 2003-05-08 | Phytex, Llc | Phytase-containing animal food and method |
CA2925807C (en) * | 2002-09-13 | 2020-09-15 | Cornell Research Foundation, Inc. | Using mutations to improve aspergillus phytases |
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US7919297B2 (en) | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
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US8540984B2 (en) | 2006-08-03 | 2013-09-24 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
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MY175858A (en) | 2012-12-19 | 2020-07-14 | Corvay Bioproducts Gmbh | Biological methods for preparing a fatty dicarboxylic acid |
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CN104195059B (zh) * | 2014-08-15 | 2016-08-24 | 陕西科技大学 | 多形汉逊酵母突变菌株及多形汉逊酵母生产d-***糖醇的方法 |
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MX2020001332A (es) | 2017-08-03 | 2020-09-09 | Synthorx Inc | Conjugados de citoquina para el tratamiento de enfermedades proliferativas e infecciosas. |
WO2020060948A1 (en) | 2018-09-17 | 2020-03-26 | Levadura Biotechnology, Inc. | Production of cannabinoids in yeast using a fatty acid feedstock |
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-
1990
- 1990-12-14 US US07/627,539 patent/US5268273A/en not_active Expired - Lifetime
-
1991
- 1991-11-14 CA CA002055542A patent/CA2055542C/en not_active Expired - Fee Related
- 1991-12-05 AU AU88823/91A patent/AU637734B2/en not_active Ceased
- 1991-12-11 ZA ZA919776A patent/ZA919776B/xx unknown
- 1991-12-12 HU HU913926A patent/HU913926D0/hu unknown
- 1991-12-13 CS CS913788A patent/CS378891A3/cs unknown
- 1991-12-13 IL IL100363A patent/IL100363A0/xx unknown
- 1991-12-13 KR KR1019910023053A patent/KR0181179B1/ko not_active IP Right Cessation
- 1991-12-13 MX MX9102546A patent/MX9102546A/es active IP Right Grant
- 1991-12-13 PL PL29276591A patent/PL292765A1/xx unknown
- 1991-12-13 DE DE69132812T patent/DE69132812T2/de not_active Expired - Fee Related
- 1991-12-13 FI FI915893A patent/FI104493B/sv active
- 1991-12-13 EP EP91121452A patent/EP0495208B1/en not_active Expired - Lifetime
- 1991-12-13 PT PT99802A patent/PT99802A/pt not_active Application Discontinuation
- 1991-12-13 NO NO19914935A patent/NO314151B1/no not_active IP Right Cessation
- 1991-12-13 YU YU193591A patent/YU193591A/sh unknown
- 1991-12-13 CN CN91111394A patent/CN1062169A/zh active Pending
- 1991-12-13 AT AT91121452T patent/ATE208822T1/de not_active IP Right Cessation
- 1991-12-13 IE IE435491A patent/IE914354A1/en not_active IP Right Cessation
- 1991-12-16 AR AR91321400A patent/AR245218A1/es active
- 1991-12-16 JP JP33233391A patent/JP3437850B2/ja not_active Expired - Lifetime
-
1992
- 1992-01-07 TW TW081100084A patent/TW201330B/zh not_active IP Right Cessation
-
1999
- 1999-11-24 FI FI992503A patent/FI113474B/sv active
Also Published As
Publication number | Publication date |
---|---|
ATE208822T1 (de) | 2001-11-15 |
NO914935D0 (no) | 1991-12-13 |
AU637734B2 (en) | 1993-06-03 |
NO314151B1 (no) | 2003-02-03 |
KR920012442A (ko) | 1992-07-27 |
EP0495208A2 (en) | 1992-07-22 |
US5268273A (en) | 1993-12-07 |
TW201330B (sv) | 1993-03-01 |
CA2055542A1 (en) | 1992-06-15 |
KR0181179B1 (ko) | 1999-04-01 |
ZA919776B (en) | 1992-09-30 |
YU193591A (sh) | 1994-06-10 |
DE69132812D1 (de) | 2001-12-20 |
CS378891A3 (en) | 1992-06-17 |
FI915893A0 (fi) | 1991-12-13 |
FI19992503A (sv) | 1999-11-24 |
CA2055542C (en) | 1999-08-31 |
HU913926D0 (en) | 1992-02-28 |
IE914354A1 (en) | 1992-06-17 |
EP0495208B1 (en) | 2001-11-14 |
JPH0576371A (ja) | 1993-03-30 |
AU8882391A (en) | 1992-06-25 |
DE69132812T2 (de) | 2002-06-20 |
IL100363A0 (en) | 1992-09-06 |
JP3437850B2 (ja) | 2003-08-18 |
MX9102546A (es) | 1992-07-01 |
FI113474B (sv) | 2004-04-30 |
PL292765A1 (en) | 1992-08-24 |
FI915893A (fi) | 1992-06-15 |
NO914935L (no) | 1992-06-15 |
PT99802A (pt) | 1992-12-31 |
AR245218A1 (es) | 1993-12-30 |
CN1062169A (zh) | 1992-06-24 |
EP0495208A3 (en) | 1994-05-18 |
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