ES2478294T3 - Sistema de agitador - Google Patents

Sistema de agitador Download PDF

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Publication number
ES2478294T3
ES2478294T3 ES10736633.8T ES10736633T ES2478294T3 ES 2478294 T3 ES2478294 T3 ES 2478294T3 ES 10736633 T ES10736633 T ES 10736633T ES 2478294 T3 ES2478294 T3 ES 2478294T3
Authority
ES
Spain
Prior art keywords
stirrer
agitator
radial
elements
mammalian cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10736633.8T
Other languages
English (en)
Inventor
Alexander Alisch
Marco Jenzsch
Michael Pohlscheidt
Joerg Thiele
Claus Wallerius
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41478681&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2478294(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2478294T3 publication Critical patent/ES2478294T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/111—Centrifugal stirrers, i.e. stirrers with radial outlets; Stirrers of the turbine type, e.g. with means to guide the flow
    • B01F27/1111—Centrifugal stirrers, i.e. stirrers with radial outlets; Stirrers of the turbine type, e.g. with means to guide the flow with a flat disc or with a disc-like element equipped with blades, e.g. Rushton turbine
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/19—Stirrers with two or more mixing elements mounted in sequence on the same axis
    • B01F27/192—Stirrers with two or more mixing elements mounted in sequence on the same axis with dissimilar elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/81—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis the stirrers having central axial inflow and substantially radial outflow
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/02—Apparatus for enzymology or microbiology with agitation means; with heat exchange means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/06—Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C12M27/08—Stirrer or mobile mixing elements with different stirrer shapes in one shaft or axis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/111—Centrifugal stirrers, i.e. stirrers with radial outlets; Stirrers of the turbine type, e.g. with means to guide the flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/113—Propeller-shaped stirrers for producing an axial flow, e.g. shaped like a ship or aircraft propeller
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/05—Stirrers
    • B01F27/11—Stirrers characterised by the configuration of the stirrers
    • B01F27/115—Stirrers characterised by the configuration of the stirrers comprising discs or disc-like elements essentially perpendicular to the stirrer shaft axis
    • B01F27/1152—Stirrers characterised by the configuration of the stirrers comprising discs or disc-like elements essentially perpendicular to the stirrer shaft axis with separate elements other than discs fixed on the discs, e.g. vanes fixed on the discs
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Sustainable Development (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Mixers Of The Rotary Stirring Type (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procedimiento de cultivo de células de mamífero, caracterizado porque las células de mamífero se cultivan en un dispositivo que comprende un sistema de agitador y un recipiente de cultivo, en el que el sistema de agitador consiste en dos elementos de a gitador de transporte radial y un elemento de agitador de transporte axial dispuestos los unos sobre los otros en un árbol de agitador vertical, en el que el elemento de agitador de transporte axial está dispuesto por encima de los elementos de agitador de transporte radial.

Description

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Claims (1)

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    imagen2
ES10736633.8T 2009-07-24 2010-07-23 Sistema de agitador Active ES2478294T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09009639 2009-07-24
EP09009639 2009-07-24
PCT/EP2010/004520 WO2011009625A1 (en) 2009-07-24 2010-07-23 Stirrer system

Publications (1)

Publication Number Publication Date
ES2478294T3 true ES2478294T3 (es) 2014-07-21

Family

ID=41478681

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10736633.8T Active ES2478294T3 (es) 2009-07-24 2010-07-23 Sistema de agitador

Country Status (14)

Country Link
US (3) US20120135464A1 (es)
EP (1) EP2456547B2 (es)
JP (5) JP5921433B2 (es)
KR (1) KR20120031083A (es)
CN (1) CN102639221B (es)
AU (1) AU2010275687A1 (es)
BR (1) BRPI1010068A2 (es)
CA (1) CA2766836A1 (es)
DE (1) DE202010018640U1 (es)
ES (1) ES2478294T3 (es)
IL (1) IL216666A0 (es)
MX (1) MX2012000730A (es)
SG (1) SG177727A1 (es)
WO (1) WO2011009625A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LT3597659T (lt) 2007-07-09 2023-05-10 Genentech, Inc. Disulfidinės jungties redukcijos prevencijos būdas gaminant polipeptidą rekombinantiniu būdu
EP2437874B1 (en) 2009-06-05 2015-05-06 F.Hoffmann-La Roche Ag Device for cultivating cells
DE202010018640U1 (de) * 2009-07-24 2019-11-14 F. Hoffmann-La Roche Ag Rührersystem
US8619148B1 (en) 2012-01-04 2013-12-31 Audience, Inc. Image correction after combining images from multiple cameras
CA2896251A1 (en) 2013-01-11 2014-07-17 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material
KR101477258B1 (ko) * 2013-05-29 2014-12-30 현대제철 주식회사 용선 교반용 임펠라의 침적 깊이 제어장치 및 제어방법
US11944946B2 (en) 2013-06-28 2024-04-02 Saint-Gobain Performance Plastics Corporation Mixing assemblies including magnetic impellers
BR112015031637B1 (pt) 2013-06-28 2022-01-18 Saint-Gobain Performance Plastics Corporation Conjuntos de mistura incluindo impelidores magnéticos
JP6696421B2 (ja) * 2014-06-04 2020-05-20 三菱ケミカル株式会社 トナー製造用連続式反応装置、連続式反応システム、微粒子製造方法、及び静電荷像現像用トナーの製造方法
FR3022804B1 (fr) * 2014-06-25 2016-07-15 Roquette Freres Arbre d'agitation pour broyeur
CA2998463A1 (en) * 2015-09-15 2017-03-23 Megakaryon Corporation Method for manufacturing platelets by rotary stirring culture method
CN106801009A (zh) * 2015-11-25 2017-06-06 衡阳屹顺化工有限公司 一种好气发酵装置
EP3430119B1 (en) * 2016-03-14 2023-06-07 Omnibrx Biotechnologies Private Limited A bioreactor system and method thereof
US20190274482A1 (en) * 2016-12-13 2019-09-12 Nestec S.A. Ergonomic whisk for food processing
JP7148279B2 (ja) * 2018-05-31 2022-10-05 花王株式会社 撹拌方法及び撹拌装置
WO2020072845A1 (en) * 2018-10-05 2020-04-09 University Of Baltimore Systems, methods and apparatus for utilizing a resuspension tank
RU2737205C1 (ru) * 2020-02-18 2020-11-26 Федеральное государственное бюджетное образовательное учреждение высшего образования "Арктический государственный агротехнологический университет" (ФГБОУ ВО Арктический ГАТУ) Аппарат для культивирования микроорганизмов
CN111570489B (zh) * 2020-06-22 2021-04-27 山东润德生物科技有限公司 一种好氧发酵过程的物料补料装置
CN111760542A (zh) * 2020-06-23 2020-10-13 武汉工程大学 一种化妆品膏状反应釜

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3445342A (en) * 1966-02-11 1969-05-20 New Brunswick Scientific Co Fermentation apparatus
JPS497160A (es) * 1972-05-15 1974-01-22
GB1584103A (en) * 1977-06-01 1981-02-04 Ranks Hovis Mcdougall Ltd Method and apparatus for promoting fermentation
US4438074A (en) 1981-07-21 1984-03-20 Phillips Petroleum Company Continuous polymerization reactor
JPS5970499U (ja) * 1982-11-05 1984-05-12 味の素株式会社 撹拌翼
JPS61200842A (ja) * 1985-02-28 1986-09-05 Sumitomo Heavy Ind Ltd 撹拌機
SE461444B (sv) 1985-11-21 1990-02-19 Boerje Skaanberg Impellerapparat foer omroerning av vaetska under dispergering av gas daeri
US5198156A (en) * 1986-02-17 1993-03-30 Imperial Chemical Industries Plc Agitators
JPS63146782A (ja) * 1986-12-09 1988-06-18 Kanegafuchi Chem Ind Co Ltd 高リパ−ゼ活性を有する菌体の生産方法
JPH0751920Y2 (ja) * 1991-05-30 1995-11-29 株式会社横山エンジニアリング 酒造米液化装置における撹拌翼
DK0590058T3 (da) 1991-06-14 2004-03-29 Genentech Inc Humaniseret heregulin-antistof
US5342763A (en) * 1992-11-23 1994-08-30 Genentech, Inc. Method for producing polypeptide via bacterial fermentation
GB9309429D0 (en) * 1993-05-07 1993-06-23 Bioscot Ltd Fermenter accessory
JP3193215B2 (ja) * 1993-11-18 2001-07-30 池袋琺瑯工業株式会社 撹拌装置
CN1044334C (zh) * 1994-12-28 1999-07-28 黄为民 搅拌釜
JPH08252445A (ja) * 1996-03-22 1996-10-01 Sumitomo Heavy Ind Ltd 攪拌機
JP3826443B2 (ja) * 1996-05-10 2006-09-27 株式会社カネカ 撹拌装置
FR2763867B1 (fr) * 1997-06-03 1999-07-30 Grande Paroisse Sa Dispositif pour melanger et dissoudre dans un liquide des granules solides, en particulier pour la production d'engrais phospho-azotes
CN103641885A (zh) 1998-05-06 2014-03-19 基因技术股份有限公司 用离子交换层析纯化蛋白质
US5972661A (en) 1998-09-28 1999-10-26 Penn State Research Foundation Mixing systems
WO2000053304A1 (en) * 1999-03-11 2000-09-14 Cobra Therapeutics Limited A vessel for mixing a cell lysate
US6250769B1 (en) 1999-09-13 2001-06-26 Clair F. Kirk Visor light cap
US6455306B1 (en) * 2000-06-09 2002-09-24 Transcyte, Inc. Transfusable oxygenating composition
CA2431230C (en) * 2000-12-20 2011-02-22 Bayer Pharmaceuticals Corporation A device and method for seed-train expansion of mammalian cells
WO2002092549A1 (en) * 2001-05-15 2002-11-21 Inca International S.P.A. Agitation system for alkylbenzene oxidation reactors
ATE549359T1 (de) 2003-04-02 2012-03-15 Hoffmann La Roche Antikörper gegen den insulinähnlichen wachstumsfaktor i-rezeptor und deren verwendungen
US7153480B2 (en) * 2003-05-22 2006-12-26 David Robert Bickham Apparatus for and method of producing aromatic carboxylic acids
ME01775B (me) 2003-11-05 2011-02-28 Glycart Biotechnology Ag Cd20 antitijela sa povećanim afinitetom vezivanja za fc receptor i efektornom funkcijom
JP4081478B2 (ja) * 2004-04-22 2008-04-23 エフ．ホフマン−ラ ロシュ アーゲー 攪拌機
CA2559496A1 (en) * 2004-04-27 2005-11-17 Baxter International Inc. Stirred-tank reactor system
CN100375782C (zh) * 2005-09-29 2008-03-19 华东理工大学 用于动物大规模细胞培养的生物反应器***
JP4860702B2 (ja) 2005-10-21 2012-01-25 エフ．ホフマン−ラ ロシュ アーゲー ポリペプチドのリコンビナント発現のための方法
US20080014203A1 (en) 2006-04-11 2008-01-17 Silke Hansen Antibodies against insulin-like growth factor I receptor and uses thereof
BRPI0716762A2 (pt) * 2006-09-13 2013-09-24 Abbott Lab melhorias da cultura celular
JP2008211979A (ja) * 2007-02-28 2008-09-18 Dic Corp ポリエステル化合物の製造方法
WO2008120643A1 (ja) * 2007-03-30 2008-10-09 Mitsui Engineering & Shipbuilding Co., Ltd. アルコール連続生産方法
TW200911740A (en) * 2007-06-01 2009-03-16 Solvay Process for manufacturing a chlorohydrin
JP2009072133A (ja) * 2007-09-21 2009-04-09 Hitachi Plant Technologies Ltd 培養槽装置、細胞培養方法及び物質生産方法
DE202010018640U1 (de) * 2009-07-24 2019-11-14 F. Hoffmann-La Roche Ag Rührersystem

Also Published As

Publication number Publication date
US20150175952A1 (en) 2015-06-25
SG177727A1 (en) 2012-02-28
JP2012533419A (ja) 2012-12-27
EP2456547B2 (en) 2024-03-06
JP2021007946A (ja) 2021-01-28
JP2017209676A (ja) 2017-11-30
JP2016073976A (ja) 2016-05-12
IL216666A0 (en) 2012-02-29
JP6827389B2 (ja) 2021-02-10
JP6505592B2 (ja) 2019-04-24
US20180187138A1 (en) 2018-07-05
CA2766836A1 (en) 2011-01-27
EP2456547B1 (en) 2014-06-04
WO2011009625A1 (en) 2011-01-27
BRPI1010068A2 (pt) 2016-03-15
EP2456547A1 (en) 2012-05-30
JP5921433B2 (ja) 2016-05-24
KR20120031083A (ko) 2012-03-29
JP2014140378A (ja) 2014-08-07
MX2012000730A (es) 2012-01-27
DE202010018640U1 (de) 2019-11-14
AU2010275687A1 (en) 2012-01-12
CN102639221B (zh) 2014-12-10
US20120135464A1 (en) 2012-05-31
CN102639221A (zh) 2012-08-15

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