ES2435092B1 - Método y sistema de cultivo celular - Google Patents
Método y sistema de cultivo celular Download PDFInfo
- Publication number
- ES2435092B1 ES2435092B1 ES201230933A ES201230933A ES2435092B1 ES 2435092 B1 ES2435092 B1 ES 2435092B1 ES 201230933 A ES201230933 A ES 201230933A ES 201230933 A ES201230933 A ES 201230933A ES 2435092 B1 ES2435092 B1 ES 2435092B1
- Authority
- ES
- Spain
- Prior art keywords
- cell culture
- culture system
- culture
- density
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
- C12M3/02—Tissue, human, animal or plant cell, or virus culture apparatus with means providing suspensions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
- C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
- C12M3/067—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means with moving or mobile filter elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La invención se enmarca dentro de los dispositivos automatizados para el cultivo celular. La invención presenta un método y un sistema de cultivo celular que permite modificar de manera controlada la densidad y el número de células en un cultivo. En concreto, se presenta un método iterativo cuyo número de etapas de iteración se modifica en función de la demanda de densidad y número de células necesarios durante la duración del cultivo.
Description
Claims (1)
-
imagen1 imagen2
Priority Applications (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201230933A ES2435092B1 (es) | 2012-06-14 | 2012-06-14 | Método y sistema de cultivo celular |
PT13731064T PT2861713T (pt) | 2012-06-14 | 2013-06-13 | Método e sistema de cultura celular |
US14/410,310 US9587216B2 (en) | 2012-06-14 | 2013-06-13 | Cell culture method and system |
CA2876523A CA2876523C (en) | 2012-06-14 | 2013-06-13 | Cell culture method and system |
DK13731064.5T DK2861713T3 (en) | 2012-06-14 | 2013-06-13 | Cell culture method and system |
PL13731064T PL2861713T3 (pl) | 2012-06-14 | 2013-06-13 | Sposób hodowli komórkowej i system |
PCT/EP2013/062293 WO2013186318A1 (en) | 2012-06-14 | 2013-06-13 | Cell culture method and system |
TR2018/16280T TR201816280T4 (tr) | 2012-06-14 | 2013-06-13 | Hücre kültürü yöntemi ve sistemi. |
EP13731064.5A EP2861713B1 (en) | 2012-06-14 | 2013-06-13 | Cell culture method and system |
CN201380042902.5A CN104704107B (zh) | 2012-06-14 | 2013-06-13 | 细胞培养方法和*** |
ES13731064.5T ES2693708T3 (es) | 2012-06-14 | 2013-06-13 | Método y sistema de cultivo celular |
HUE13731064A HUE039701T2 (hu) | 2012-06-14 | 2013-06-13 | Sejtkultúra-eljárás és sejtkultúra-rendszer |
US15/419,492 US10494599B2 (en) | 2012-06-14 | 2017-01-30 | Cell culture method and system |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES201230933A ES2435092B1 (es) | 2012-06-14 | 2012-06-14 | Método y sistema de cultivo celular |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2435092A1 ES2435092A1 (es) | 2013-12-18 |
ES2435092B1 true ES2435092B1 (es) | 2014-09-09 |
Family
ID=48692438
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES201230933A Active ES2435092B1 (es) | 2012-06-14 | 2012-06-14 | Método y sistema de cultivo celular |
ES13731064.5T Active ES2693708T3 (es) | 2012-06-14 | 2013-06-13 | Método y sistema de cultivo celular |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES13731064.5T Active ES2693708T3 (es) | 2012-06-14 | 2013-06-13 | Método y sistema de cultivo celular |
Country Status (11)
Country | Link |
---|---|
US (2) | US9587216B2 (es) |
EP (1) | EP2861713B1 (es) |
CN (1) | CN104704107B (es) |
CA (1) | CA2876523C (es) |
DK (1) | DK2861713T3 (es) |
ES (2) | ES2435092B1 (es) |
HU (1) | HUE039701T2 (es) |
PL (1) | PL2861713T3 (es) |
PT (1) | PT2861713T (es) |
TR (1) | TR201816280T4 (es) |
WO (1) | WO2013186318A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE1022269B1 (fr) * | 2015-02-09 | 2016-03-08 | Univercells Sa | Système, appareil et procédé pour la production de cellules et/ou de produits cellulaires |
US20180030398A1 (en) * | 2015-02-09 | 2018-02-01 | Univercells Nv | System, apparatus and method for the production of cells and/or cell products |
KR102050852B1 (ko) * | 2016-03-11 | 2019-12-03 | 주식회사 스템온 | 세포 리프로그래밍 장치 |
EP3556841A1 (en) * | 2018-04-19 | 2019-10-23 | Aglaris Ltd | Disposable cartridge cooperating with a platform in a flexible system for handling and/or manipulating fluids |
CN112852616B (zh) * | 2021-01-22 | 2024-06-25 | 福建省微生物研究所 | 一种流体生物反应器及其使用方法 |
CN114350591A (zh) * | 2022-01-13 | 2022-04-15 | 中山大学附属第一医院 | 一种在压力条件下培养细胞的方法 |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5202254A (en) * | 1990-10-11 | 1993-04-13 | Endotronics, Inc. | Process for improving mass transfer in a membrane bioreactor and providing a more homogeneous culture environment |
US5989913A (en) | 1998-07-02 | 1999-11-23 | Charles Daniel Anderson | Culture vessel for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using the same |
DE19952847B4 (de) * | 1999-10-01 | 2006-03-23 | Minuth, Will, Prof. Dr. | Vorrichtung zum Kultivieren und/oder Differenzieren und/oder Halten von Zellen und/oder Geweben |
EP1279728A1 (en) * | 2001-07-27 | 2003-01-29 | Schuler, Gerold | Generation of fully mature and stable dentritic cells from leukapheresis products for clinical applications |
US20040214314A1 (en) * | 2001-11-02 | 2004-10-28 | Friedrich Srienc | High throughput bioreactor |
EP1354941A1 (en) * | 2002-04-19 | 2003-10-22 | Computer Cell Culture Center S.A. | Apparatus and method for a cell culture in a bioreactor at high cell concentration |
US7507579B2 (en) | 2002-05-01 | 2009-03-24 | Massachusetts Institute Of Technology | Apparatus and methods for simultaneous operation of miniaturized reactors |
US20050170491A1 (en) * | 2002-07-31 | 2005-08-04 | Mutsumi Takagi | Automatic culture apparatus for cell or tisse with biological origin |
CN1249217C (zh) * | 2003-01-27 | 2006-04-05 | 英科新创(厦门)科技有限公司 | 细胞培养方法和装置 |
CN101416059A (zh) | 2003-07-17 | 2009-04-22 | 格洛伯塞尔解决方案公司 | 自动化细胞培养***及方法 |
ITPI20040046A1 (it) | 2004-06-18 | 2004-09-18 | Univ Pisa | Bioreattore per lo studio degli effetti sulle attivita' cellulari di stimoli imposti |
WO2007044699A1 (en) | 2005-10-07 | 2007-04-19 | Massachusetts Institute Of Technology | Parallel integrated bioreactor device and method |
US8951784B2 (en) * | 2005-12-14 | 2015-02-10 | Sepragen Corporation | Cell culture bioreactor |
US20100317102A1 (en) * | 2006-01-17 | 2010-12-16 | Tsutomu Suzuki | Cell Culture Method and Automatic Culture System Using the Method |
WO2007139748A2 (en) | 2006-05-22 | 2007-12-06 | Biovest International Inc. | Extra-capillary fluid cycling system and method for a cell culture device |
JP2008228585A (ja) * | 2007-03-16 | 2008-10-02 | Canon Inc | 細胞培養容器および細胞培養装置 |
DK2370561T3 (da) * | 2008-12-16 | 2019-10-21 | Emd Millipore Corp | Omrøringstankreaktor og fremgangsmåde |
WO2010103748A1 (ja) * | 2009-03-09 | 2010-09-16 | 東洋製罐株式会社 | 細胞培養方法、細胞培養装置、容器内の計数対象物の計数方法、及び計数用装置 |
US20120034596A1 (en) * | 2009-04-22 | 2012-02-09 | Pan-Systech Gmbh | Device for automatically cultivating cells in parallel |
GB201007261D0 (en) | 2010-04-30 | 2010-06-16 | Isis Innovation | Reactor |
DE102010026369B4 (de) * | 2010-07-07 | 2015-07-30 | Zellwerk Gmbh | Verfahren und Druck-Drehbett-Bioreaktor zur dynamischen Expansion und/oder Differenzierung von Stammzellen oder primären Zellen humanen und tierischen Ursprungs |
US9260686B2 (en) * | 2010-10-08 | 2016-02-16 | University Of Maryland, College Park | Tubular bioreactor system for use in bone and cartilage tissue engineering |
-
2012
- 2012-06-14 ES ES201230933A patent/ES2435092B1/es active Active
-
2013
- 2013-06-13 TR TR2018/16280T patent/TR201816280T4/tr unknown
- 2013-06-13 PT PT13731064T patent/PT2861713T/pt unknown
- 2013-06-13 ES ES13731064.5T patent/ES2693708T3/es active Active
- 2013-06-13 EP EP13731064.5A patent/EP2861713B1/en active Active
- 2013-06-13 US US14/410,310 patent/US9587216B2/en active Active
- 2013-06-13 DK DK13731064.5T patent/DK2861713T3/en active
- 2013-06-13 CA CA2876523A patent/CA2876523C/en active Active
- 2013-06-13 PL PL13731064T patent/PL2861713T3/pl unknown
- 2013-06-13 WO PCT/EP2013/062293 patent/WO2013186318A1/en active Application Filing
- 2013-06-13 HU HUE13731064A patent/HUE039701T2/hu unknown
- 2013-06-13 CN CN201380042902.5A patent/CN104704107B/zh not_active Expired - Fee Related
-
2017
- 2017-01-30 US US15/419,492 patent/US10494599B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CN104704107A (zh) | 2015-06-10 |
CN104704107B (zh) | 2017-11-24 |
TR201816280T4 (tr) | 2018-11-21 |
HUE039701T2 (hu) | 2019-02-28 |
CA2876523A1 (en) | 2013-12-19 |
PL2861713T3 (pl) | 2019-01-31 |
US10494599B2 (en) | 2019-12-03 |
EP2861713B1 (en) | 2018-08-08 |
CA2876523C (en) | 2021-10-19 |
ES2693708T3 (es) | 2018-12-13 |
US20150159128A1 (en) | 2015-06-11 |
DK2861713T3 (en) | 2018-11-19 |
US9587216B2 (en) | 2017-03-07 |
PT2861713T (pt) | 2018-11-16 |
ES2435092A1 (es) | 2013-12-18 |
EP2861713A1 (en) | 2015-04-22 |
WO2013186318A1 (en) | 2013-12-19 |
US20170298317A1 (en) | 2017-10-19 |
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