Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SAfiledCriticalAntibioticos SA
Priority to ES534757ApriorityCriticalpatent/ES8602933A1/es
Publication of ES534757A0publicationCriticalpatent/ES534757A0/es
Publication of ES8602933A1publicationCriticalpatent/ES8602933A1/es
Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
PROCEDIMIENTO PARA CLONAR EN MICROORGANISMOS LOS GENES QUE CODIFICAN PARA LAS PROTEINAS CON ACTIVIDAD DE PENICILINACILASA Y PRODUCIRLAS POR FERMENTACION. COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE SELECCIONAN MICROORGANISMOS PRODUCTORES DE PENICILINACILASA Y SE AISLAN DE ELLOS LOS FRAGMENTOS DE DNA QUE CONTIENEN LA INFORMACION GENETICA RELATIVA A LA PRODUCCION DE PENICILINACILASA Y SE INSERTAN EN UN VECTOR DE DNA PARA OBTENER LOS CORRESPONDIENTES VECTORES RECOMBINANTES; SEGUNDA, SE INTRODUCEN DICHOS VECTORES EN LAS CELULAS DE UN MICROORGANISMO HUESPED, NO PRODUCTOR DE PENICILINACILASA, DE CEPAS AUXOTROFAS PARA COMPUESTOS CAPACES DE ORIGINAR ENLACES AMIDA CON EL ACIDO FENILACETICO; Y POR ULTIMO, SE AISLAN LOS TRANSFORMANTES CON ACTIVIDAD DE PENICILINACILASA Y SE CULTIVAN LAS CELULAS TRANSFORMANTES EN UN MEDIO DE CULTIVO APROPIADO.
ES534757A1984-07-311984-07-31Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion
ExpiredES8602933A1
(es)
Nuevas secuencias nucleotidicas codificadoras de gen thre y procedimiento para la preparacion por fermentacion de l-treonina utilizando bacterias corineformes.
Polynucleotide sequences, polypeptides coded by them and exhibiting activity resulting in exposrting branched-chain amino acids, host cell an method of producing branched-chain amino acids
Bacillus subtilis strain whose extracellular protease activities are reduced, method for obtaining the strain and method for secreting proteins by using the strain