ES2132044T1 - Inmunoensayo para detectar h. pylori en muestras fecales. - Google Patents
Inmunoensayo para detectar h. pylori en muestras fecales.Info
- Publication number
- ES2132044T1 ES2132044T1 ES97303147T ES97303147T ES2132044T1 ES 2132044 T1 ES2132044 T1 ES 2132044T1 ES 97303147 T ES97303147 T ES 97303147T ES 97303147 T ES97303147 T ES 97303147T ES 2132044 T1 ES2132044 T1 ES 2132044T1
- Authority
- ES
- Spain
- Prior art keywords
- pylori
- antibody
- sample
- antigen
- detect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56922—Campylobacter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
SE DESCRIBE UN INMUNOENSAYO PARA H. PYLORI EN MUESTRAS FECALES. SE DISPERSA UNA MUESTRA DE HECES SOSPECHOSA DE CONTENER H. PYLORI EN UN DILUYENTE DE MUESTRA. LA MUESTRA FECAL EN EL DILUYENTE SE PONE EN CONTACTO CON UN PRIMER ANTICUERPO POLICLONAL PARA ANTIGENO H. PYLORI PARA FORMAR UN COMPLEJO DEL ANTICUERPO Y EL ANTIGENO. SE SEPARAN LA MUESTRA Y EL COMPLEJO; ESTE SE EXPONE A UN SEGUNDO ANTICUERPO POLICLONAL PARA EL ANTIGENO Y UNA PORCION DEL ANTICUERPO QUE REACCIONA CON EL COMPLEJO. EL PRIMERO O EL SEGUNDO ANTICUERPO SE UNE A UN SOPORTE SOLIDO Y EL OTRO SE MARCA CON UN AGENTE DE DETECCION. SE DETERMINA LA CANTIDAD DE ANTICUERPO MARCADO Y A PARTIR DE ESTA LA PRESENCIA DE ANTIGENO H. PYLORI EN LA MUESTRA FECAL.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US647115 | 1996-05-09 | ||
US08/647,115 US5716791A (en) | 1996-05-09 | 1996-05-09 | Immunoassay for H. pylori in fecal specimens |
Publications (3)
Publication Number | Publication Date |
---|---|
ES2132044T1 true ES2132044T1 (es) | 1999-08-16 |
ES2132044T3 ES2132044T3 (es) | 2002-03-16 |
ES2132044T5 ES2132044T5 (es) | 2008-12-01 |
Family
ID=24595765
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES97303147T Expired - Lifetime ES2132044T5 (es) | 1996-05-09 | 1997-05-08 | Inmunoensayo para h. pylori en muestras fecales. |
Country Status (12)
Country | Link |
---|---|
US (3) | US5716791A (es) |
EP (1) | EP0806667B2 (es) |
JP (1) | JP3043999B2 (es) |
CN (1) | CN1271408C (es) |
AR (1) | AR006016A1 (es) |
AT (1) | ATE211552T1 (es) |
AU (1) | AU698513B2 (es) |
CA (1) | CA2198336C (es) |
DE (2) | DE69709434T3 (es) |
ES (1) | ES2132044T5 (es) |
GR (1) | GR990300022T1 (es) |
MX (1) | MX9701877A (es) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
CA2224551A1 (en) * | 1998-02-25 | 1999-08-25 | Alex Hongsheng Chang | A quick in situ diagnostic method for detecting alimentary diseases |
EA004107B1 (ru) | 1998-08-11 | 2003-12-25 | Айдек Фармацевтикалс Корпорэйшн | Комбинированная терапия в-клеточных лимфом, предусматривающая введение антитела против cd20 |
AU1157100A (en) | 1998-10-29 | 2000-05-22 | Connex Gmbh | Detection of acid-resistant micro-organisms in a stool |
US8557244B1 (en) | 1999-08-11 | 2013-10-15 | Biogen Idec Inc. | Treatment of aggressive non-Hodgkins lymphoma with anti-CD20 antibody |
BR0014734A (pt) | 1999-10-12 | 2002-06-18 | Connex Ges Zur Optimierung Von | Método melhorado para detectar microorganismos resistentes a ácido na evacuação |
JP3504633B2 (ja) * | 1999-10-29 | 2004-03-08 | わかもと製薬株式会社 | ヘリコバクター・ピロリへの感染を判定する検査方法及び検査試薬 |
CN1195869C (zh) | 1999-10-29 | 2005-04-06 | 若素制药株式会社 | 单克隆抗体、杂交瘤、免疫测定法和诊断试剂盒 |
AU1939501A (en) * | 1999-12-03 | 2001-06-12 | Meridian Bioscience, Inc. | Diagnosis and treatment for helicobacter pylori induced colic |
TWI237695B (en) * | 1999-12-14 | 2005-08-11 | Joy Biomedical Corp | Helicobacter pylori antigens in blood |
DE10006432A1 (de) * | 2000-02-14 | 2001-08-16 | Ganzimmun Inst Fuer Ganzheitli | Verfahren zum Nachweis von Helicobacter pylori in Stuhl- und Speichelproben |
ATE434185T1 (de) * | 2000-05-18 | 2009-07-15 | Meridian Bioscience Inc | Immunoassay für h. pylori in fäkalienproben mit hilfe von gattungsspezifischen antikörpern |
WO2002014541A1 (en) * | 2000-08-11 | 2002-02-21 | Wakamoto Pharmaceutical Co., Ltd. | Method of examining infection with helicobacter pylori and diagnostic kit |
JP4443117B2 (ja) * | 2001-04-23 | 2010-03-31 | わかもと製薬株式会社 | イムノクロマトグラフィー試験片及び診断キット |
JP4763149B2 (ja) * | 2001-05-10 | 2011-08-31 | わかもと製薬株式会社 | ヘリコバクター・ピロリへの感染を判定する検査方法 |
FI118061B (fi) | 2001-09-24 | 2007-06-15 | Beanor Oy | Menetelmä ja bioanturi analyysiä varten |
FI115166B (fi) | 2001-12-31 | 2005-03-15 | Biofons Oy | Diagnostisia menetelmiä |
DE10219741A1 (de) * | 2002-05-02 | 2003-11-13 | Georg S Wengler | Verfahren zur Vorbehandlung von Stuhlproben |
KR20040046427A (ko) * | 2002-11-27 | 2004-06-05 | 주식회사 비엘에스 | 분변 시료 중의 헬리코박터 피로리의 검출방법 |
JP2010510526A (ja) * | 2006-11-22 | 2010-04-02 | スリーエム イノベイティブ プロパティズ カンパニー | 細菌全細胞を捕捉する方法及び細菌について試料を分析する方法 |
US8025880B2 (en) * | 2007-03-01 | 2011-09-27 | Helicure Ab | Immunoglobulin against Helicobacter pylori |
US20090263905A1 (en) | 2008-04-18 | 2009-10-22 | Kim Scheuringer | Detection test assembly for detecting the presence of a substance in a sample |
US9110053B2 (en) * | 2010-08-20 | 2015-08-18 | Agilent Technologies, Inc. | Dried blood spotting paper device and method |
US8541180B1 (en) | 2010-10-11 | 2013-09-24 | Genova Diagnostics, Inc. | Compositions and methods for assessing gastrointestinal health |
CN102565391A (zh) * | 2011-12-23 | 2012-07-11 | 王滔 | 胃黏膜样品中幽门螺杆菌的免疫测定法 |
CN103293305A (zh) * | 2012-03-05 | 2013-09-11 | 美利泰格诊断试剂(嘉兴)有限公司 | 口腔螺旋杆菌感染检测方法及用于检测的唾液试板 |
CN102980884A (zh) * | 2012-11-29 | 2013-03-20 | 江苏创生生物技术有限公司 | 胃黏膜样品中幽门螺旋杆菌的化学发光免疫测定法 |
CN103149067A (zh) * | 2013-02-28 | 2013-06-12 | 苏州和锐医药科技有限公司 | 一种粪便蛋白萃取方法及试剂 |
KR101619862B1 (ko) | 2014-09-30 | 2016-05-12 | 주식회사 녹십자 | 간접효소면역측정법을 이용하는 인간 Fc 포함 단백질의 역가 측정키트 및 이를 이용한 Fc 포함 단백질의 역가 측정방법 |
DE102015000626A1 (de) * | 2015-01-22 | 2016-07-28 | Kibion Gmbh | Verfahren zum Nachweis von Helicobacter Pylori |
CN109342722A (zh) * | 2018-09-30 | 2019-02-15 | 深圳市鸿美诊断技术有限公司 | 一种用于定量测定粪便中幽门螺杆菌抗原试剂的制备方法 |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR231590A1 (es) * | 1981-04-29 | 1984-12-28 | Ciba Geigy Ag | Dispositivo de analisis inmunologico y procedimiento para obtenerlo |
JP2607496B2 (ja) * | 1986-01-22 | 1997-05-07 | アモコ・コーポレーション | カンピロバクター検出用プローブ |
US4942126A (en) * | 1986-08-13 | 1990-07-17 | Allegheny-Singer Research Institute | Method of extracting antigens of bacteria |
EP0281251A3 (en) * | 1987-02-04 | 1988-09-28 | International Immunoassay Laboratories, Inc. | A method for preparing a fecal sample composition for immunoassay testing |
US4923801A (en) * | 1987-04-13 | 1990-05-08 | The University Of Virginia Alumni Patents Foundation | Compositions and methods for the enrichment and isolation of Campylobacter pylori and related organisms from biological specimens and the environment |
US5459041A (en) * | 1988-02-18 | 1995-10-17 | Enteric Research Laboratories, Inc. | Campylobacter pylori antigens and uses thereof for detection of Campylobacter pylori infection |
US4882271A (en) * | 1988-03-10 | 1989-11-21 | Baylor College Of Medicine | Process for preparation of high molecular weight cell-associated protein of campylobacter pylori and use for serological detection of campylobacter pylori infection |
US5017342A (en) * | 1988-06-01 | 1991-05-21 | Ortho Diagnostic Systems Inc. | Device for immunoassay determinations |
US5571674A (en) * | 1989-09-18 | 1996-11-05 | The Trustees Of Columbia University In The City Of New York | DNA oligomers for use in detection of campylobacter pylori and methods of using such DNA oligomers |
AU644121B2 (en) * | 1989-12-04 | 1993-12-02 | Auspharm International Limited | Rapid in vitro test for helicobacter pylori using saliva |
JP2988635B2 (ja) * | 1990-09-18 | 1999-12-13 | 塩野義製薬株式会社 | ヒトIgEに対するモノクローナル抗体 |
US5262156A (en) * | 1991-08-12 | 1993-11-16 | Hycor Biomedical, Inc. | Antigenic compositions and their use for the detection of Helicobacter pylori |
US5420014A (en) * | 1992-04-30 | 1995-05-30 | Auspharm International Ltd. | Rapid in vitro test for helicobacter pylori using saliva |
PL172125B1 (pl) * | 1992-04-29 | 1997-08-29 | Auspharm Int Ltd | Sposób wykrywania trwajacych infekcji Helicobacter pyroli w komórkach ssaków PL PL PL |
US5403924A (en) * | 1992-10-13 | 1995-04-04 | Vanderbilt University | Taga gene and methods for detecting predisposition to peptic ulceration |
JPH07509783A (ja) * | 1992-10-30 | 1995-10-26 | ティー セル ダイアグノスティックス,インコーポレーテッド | 試料中の全分子の測定及びそれに基づく方法 |
GB9313437D0 (en) † | 1993-06-30 | 1993-08-11 | Smith Andrew W | Pathogenicity sequences in helicobacter pylori |
US5486361A (en) * | 1993-10-25 | 1996-01-23 | The United States Of America As Represented By The Department Of Health And Human Services | Hybridomas and monoclonal antibodies that speifically bind to GPIB on platelets and inhibit the binding of thrombin to platelets |
US5498528A (en) * | 1994-06-10 | 1996-03-12 | King; Wing | Detection of helicobacter pylori |
US5610060A (en) * | 1994-06-24 | 1997-03-11 | The United States Of America As Represented By The Department Of Health And Human Services | Isolated Helicobacter hepaticus |
US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
-
1996
- 1996-05-09 US US08/647,115 patent/US5716791A/en not_active Ceased
-
1997
- 1997-02-24 CA CA002198336A patent/CA2198336C/en not_active Expired - Lifetime
- 1997-02-27 AR ARP970100781A patent/AR006016A1/es active IP Right Grant
- 1997-02-28 AU AU15029/97A patent/AU698513B2/en not_active Expired
- 1997-03-12 MX MX9701877A patent/MX9701877A/es unknown
- 1997-03-12 CN CN97103112.6A patent/CN1271408C/zh not_active Expired - Lifetime
- 1997-03-17 JP JP9062637A patent/JP3043999B2/ja not_active Expired - Fee Related
- 1997-05-08 DE DE69709434T patent/DE69709434T3/de not_active Expired - Lifetime
- 1997-05-08 AT AT97303147T patent/ATE211552T1/de not_active IP Right Cessation
- 1997-05-08 ES ES97303147T patent/ES2132044T5/es not_active Expired - Lifetime
- 1997-05-08 DE DE0806667T patent/DE806667T1/de active Pending
- 1997-05-08 EP EP97303147A patent/EP0806667B2/en not_active Expired - Lifetime
- 1997-07-21 US US08/897,732 patent/US5871942A/en not_active Expired - Lifetime
-
1999
- 1999-01-01 GR GR990300022T patent/GR990300022T1/el unknown
-
2000
- 2000-02-08 US US09/500,365 patent/USRE38088E1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0806667B1 (en) | 2002-01-02 |
EP0806667B2 (en) | 2008-07-09 |
DE69709434T2 (de) | 2002-09-19 |
AR006016A1 (es) | 1999-07-21 |
GR990300022T1 (en) | 1999-07-30 |
DE806667T1 (de) | 1999-10-07 |
JP3043999B2 (ja) | 2000-05-22 |
EP0806667A1 (en) | 1997-11-12 |
CN1271408C (zh) | 2006-08-23 |
CA2198336A1 (en) | 1997-11-10 |
JPH1010128A (ja) | 1998-01-16 |
DE69709434T3 (de) | 2009-01-02 |
US5871942A (en) | 1999-02-16 |
CA2198336C (en) | 2002-06-18 |
ES2132044T5 (es) | 2008-12-01 |
AU1502997A (en) | 1997-11-13 |
ES2132044T3 (es) | 2002-03-16 |
US5716791A (en) | 1998-02-10 |
AU698513B2 (en) | 1998-10-29 |
ATE211552T1 (de) | 2002-01-15 |
USRE38088E1 (en) | 2003-04-22 |
DE69709434D1 (de) | 2002-02-07 |
CN1165299A (zh) | 1997-11-19 |
MX9701877A (es) | 1998-04-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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