ES2088374T3 - Purificacion de una proteina de membrana externa de pertussis. - Google Patents
Purificacion de una proteina de membrana externa de pertussis.Info
- Publication number
- ES2088374T3 ES2088374T3 ES91906730T ES91906730T ES2088374T3 ES 2088374 T3 ES2088374 T3 ES 2088374T3 ES 91906730 T ES91906730 T ES 91906730T ES 91906730 T ES91906730 T ES 91906730T ES 2088374 T3 ES2088374 T3 ES 2088374T3
- Authority
- ES
- Spain
- Prior art keywords
- pertactine
- precipitate
- haf
- extract
- precipitated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 201000005702 Pertussis Diseases 0.000 title abstract 2
- 108010052285 Membrane Proteins Proteins 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 239000002244 precipitate Substances 0.000 abstract 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 2
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 2
- 239000001166 ammonium sulphate Substances 0.000 abstract 2
- 241000588832 Bordetella pertussis Species 0.000 abstract 1
- 229920002684 Sepharose Polymers 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
- 102000030621 adenylate cyclase Human genes 0.000 abstract 1
- 108060000200 adenylate cyclase Proteins 0.000 abstract 1
- 239000004202 carbamide Substances 0.000 abstract 1
- 238000004090 dissolution Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/235—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bordetella (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Oncology (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
SE RECUPERA LA PERTACTINA (ANTERIORMENTE PROTEINA 69 KDA) EN UNA FORMA BIOLOGICAMENTE PURA ESTABLE SIN ACTIVIDAD DETECTABLE DE CICLASA DE ADENILATO A PARTIR DEL CALDO DE FERMENTACION DEL BORDETELLA PERTUSSI ASI COMO A PARTIR DE CELULAS. SE TRATA EL CALDO PARA EXTRAER SELECTIVAMENTE LA TOXINA PERTUSSI (TP) Y LA HEMAGLUTININA FILAMENTOSA (HAF), SE PRECIPITA LA PERTACTINA CON SULFATO DE AMONIO Y SE DISUELVE EL PRECIPITADO EN TAMPON A UN PH DE 0,6 A 8,5, A CONTINUACION SE PASA LA DISOLUCION A TRAVES DE HIDROXIAPATITA Y A LAS COLUMNAS CROMATOGRAFICAS DE Q-SEPHAROSE ANTES DE LA ULTRAFILTRACION FINAL. SE EXTRAEN LAS CELULAS CON UREA Y EL EXTRACTO ULTRAFILTRADO Y DIAFILTRADO. SE PRECIPITA LA PERTACTINA A PARTIR DEL EXTRACTO Y SE PROCESA EL PRECIPITADO DE LA FORMA DESCRITA ANTERIORMENTE. EN UNA VARIACION, SE PONE EN CONTACTO EL CALDO CON SULFATO DE AMONIO PARA PRECIPITAR LA PERTACTINA, LA TP Y LA HAF, SE DISUELVE EL PRECIPITADO Y SE EXTRAEN SELECTIVAMENTE LA TP Y LA HAF ANTES DE PASAR LA DISOLUCIONA LAS COLUMNAS CROMATOGRAFICAS.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB909007657A GB9007657D0 (en) | 1990-04-04 | 1990-04-04 | Purification of a pertussis outer membrane protein(omp69) |
| GB9007657 | 1990-04-04 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ES2088374T1 ES2088374T1 (es) | 1996-08-16 |
| ES2088374T3 true ES2088374T3 (es) | 1997-05-16 |
| ES2088374T5 ES2088374T5 (es) | 2004-08-16 |
Family
ID=10673904
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91906730T Expired - Lifetime ES2088374T5 (es) | 1990-04-04 | 1991-04-03 | Purificacion y uso de pertactina, una proteina de membrana externa de bordetella pertussis. |
| ES96202627T Pending ES2104535T1 (es) | 1990-04-04 | 1991-04-03 | Vacuna que contiene proteina de membrana externa de bordetella pertussis, y metodo para elaborar tal vacuna. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES96202627T Pending ES2104535T1 (es) | 1990-04-04 | 1991-04-03 | Vacuna que contiene proteina de membrana externa de bordetella pertussis, y metodo para elaborar tal vacuna. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US5444159A (es) |
| EP (2) | EP0527753B2 (es) |
| JP (1) | JP2549225B2 (es) |
| AT (1) | ATE147754T1 (es) |
| CA (1) | CA2079887C (es) |
| DE (3) | DE761230T1 (es) |
| DK (1) | DK0527753T4 (es) |
| ES (2) | ES2088374T5 (es) |
| GB (1) | GB9007657D0 (es) |
| GR (2) | GR3022769T3 (es) |
| WO (1) | WO1991015505A1 (es) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8412207D0 (en) * | 1984-05-12 | 1984-06-20 | Wellcome Found | Antigenic preparations |
| GB8910570D0 (en) | 1989-05-08 | 1989-06-21 | Wellcome Found | Acellular vaccine |
| US6197548B1 (en) | 1990-04-02 | 2001-03-06 | Medeva Pharma Limited | Transformed Pichia expressing the pertactin antigen |
| NL9002092A (nl) * | 1990-09-25 | 1992-04-16 | Nederlanden Staat | Vaccin, geschikt voor de bestrijding van bordetella pertussis. |
| US6444211B2 (en) * | 1991-04-03 | 2002-09-03 | Connaught Laboratories, Inc. | Purification of a pertussis outer membrane protein |
| GB9304399D0 (en) * | 1993-03-04 | 1993-04-21 | Smithkline Beecham Biolog | Novel process |
| DE69511392T2 (de) * | 1994-04-28 | 2000-03-23 | Takeda Chemical Industries Ltd | Verfahren zur trennung von protektiven verbindungen aus bordetella pertussis |
| US5877298A (en) * | 1995-05-04 | 1999-03-02 | Connaught Lab | Acellular pertussis vaccines and methods of preparing thereof |
| EP1233022B1 (en) * | 1995-05-04 | 2015-12-09 | Aventis Pasteur Limited | Methods of preparation of components of acellular pertussis vaccines |
| JP3745805B2 (ja) * | 1995-10-24 | 2006-02-15 | 日本ケミカルリサーチ株式会社 | トロンボモジュリンの精製方法 |
| US5739313A (en) | 1995-11-13 | 1998-04-14 | Regents Of The University Of Minnesota | Radionuclide labeling of vitamin B12 and coenzymes thereof |
| AU724743B2 (en) * | 1996-05-31 | 2000-09-28 | Genetics Institute, Llc | IL-12 as an adjuvant for Bordetella Pertussis vaccines |
| EP1762246A1 (en) | 1996-07-02 | 2007-03-14 | Sanofi Pasteur Limited | Multivalent DTP-Polio vaccines |
| DE60231651D1 (de) * | 2001-12-21 | 2009-04-30 | Immunex Corp | Proteinreinigungsverfahren |
| WO2009016651A1 (en) * | 2007-07-31 | 2009-02-05 | Panacea Biotec Limited | Simplified means for obtaining prn from bordetella pertussis |
| US8574589B2 (en) | 2009-05-11 | 2013-11-05 | Novartis Ag | Antigen purification process for pertactin antigen |
| CN102584958A (zh) * | 2012-02-08 | 2012-07-18 | 北京天坛生物制品股份有限公司 | 百日咳杆菌69kd外膜蛋白的纯化方法 |
| DK2863943T3 (en) | 2013-03-08 | 2016-11-07 | Janssen Vaccines & Prevention Bv | acellular pertussis vaccines |
| KR102426041B1 (ko) * | 2017-08-01 | 2022-07-29 | 주식회사 녹십자 | 냉동 및 해동 과정을 포함하는 백일해균 유래 단백질 수득 방법 |
| CN113151081A (zh) * | 2021-04-21 | 2021-07-23 | 深圳市儿童医院 | 一种百日咳鲍特菌培养基及其制备方法 |
| WO2024056508A1 (en) | 2022-09-15 | 2024-03-21 | Cube Biotech Gmbh | In vitro diagnostic method for detecting the presence of a target by using stabilized membrane proteins |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5237052A (en) * | 1984-05-12 | 1993-08-17 | Burroughs Wellcome Company | Antigenic preparations and isolation of such preparations |
| GB8412207D0 (en) * | 1984-05-12 | 1984-06-20 | Wellcome Found | Antigenic preparations |
| GB8807860D0 (en) * | 1988-04-05 | 1988-05-05 | Connaught Lab | Pertussis vaccine |
| US5101014A (en) * | 1989-02-10 | 1992-03-31 | United States Of America | Process for the purification of a 69,000 da outer membrane protein of Bordetella pertussis |
| US5276142A (en) * | 1989-12-11 | 1994-01-04 | American Cyanamid Company | Process for purification of a 69000 dalton antigenic protein from Bordetella pertussis |
| GB9007416D0 (en) † | 1990-04-02 | 1990-05-30 | Wellcome Found | Expression of heterologous protein in yeast |
-
1990
- 1990-04-04 GB GB909007657A patent/GB9007657D0/en active Pending
-
1991
- 1991-04-03 EP EP91906730A patent/EP0527753B2/en not_active Expired - Lifetime
- 1991-04-03 JP JP3506712A patent/JP2549225B2/ja not_active Expired - Lifetime
- 1991-04-03 DE DE0761230T patent/DE761230T1/de active Pending
- 1991-04-03 WO PCT/CA1991/000110 patent/WO1991015505A1/en active IP Right Grant
- 1991-04-03 DE DE69124235T patent/DE69124235T3/de not_active Expired - Lifetime
- 1991-04-03 EP EP96202627A patent/EP0761230A3/en not_active Withdrawn
- 1991-04-03 DE DE0527753T patent/DE527753T1/de active Pending
- 1991-04-03 CA CA002079887A patent/CA2079887C/en not_active Expired - Lifetime
- 1991-04-03 AT AT91906730T patent/ATE147754T1/de not_active IP Right Cessation
- 1991-04-03 ES ES91906730T patent/ES2088374T5/es not_active Expired - Lifetime
- 1991-04-03 DK DK91906730T patent/DK0527753T4/da active
- 1991-04-03 ES ES96202627T patent/ES2104535T1/es active Pending
-
1992
- 1992-11-06 US US07/930,595 patent/US5444159A/en not_active Expired - Lifetime
-
1995
- 1995-05-04 US US08/433,644 patent/US5667787A/en not_active Expired - Lifetime
-
1997
- 1997-03-07 GR GR970400453T patent/GR3022769T3/el unknown
- 1997-10-31 GR GR970300034T patent/GR970300034T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2104535T1 (es) | 1997-10-16 |
| ATE147754T1 (de) | 1997-02-15 |
| CA2079887C (en) | 1998-06-23 |
| EP0527753B1 (en) | 1997-01-15 |
| ES2088374T1 (es) | 1996-08-16 |
| DE761230T1 (de) | 1997-09-25 |
| US5667787A (en) | 1997-09-16 |
| ES2088374T5 (es) | 2004-08-16 |
| JPH05506649A (ja) | 1993-09-30 |
| GB9007657D0 (en) | 1990-05-30 |
| GR3022769T3 (en) | 1997-06-30 |
| DE69124235D1 (de) | 1997-02-27 |
| EP0527753B2 (en) | 2003-12-17 |
| DK0527753T3 (da) | 1997-04-07 |
| DE69124235T2 (de) | 1997-05-28 |
| DE69124235T3 (de) | 2004-06-03 |
| GR970300034T1 (en) | 1997-10-31 |
| WO1991015505A1 (en) | 1991-10-17 |
| EP0527753A1 (en) | 1993-02-24 |
| US5444159A (en) | 1995-08-22 |
| DE527753T1 (de) | 1996-11-28 |
| JP2549225B2 (ja) | 1996-10-30 |
| EP0761230A2 (en) | 1997-03-12 |
| EP0761230A3 (en) | 1999-01-20 |
| CA2079887A1 (en) | 1991-10-05 |
| DK0527753T4 (da) | 2004-03-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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