EP2826862A4 - Procédé de synthèse de gènes, puce à adn et kit - Google Patents
Procédé de synthèse de gènes, puce à adn et kitInfo
- Publication number
- EP2826862A4 EP2826862A4 EP12871271.8A EP12871271A EP2826862A4 EP 2826862 A4 EP2826862 A4 EP 2826862A4 EP 12871271 A EP12871271 A EP 12871271A EP 2826862 A4 EP2826862 A4 EP 2826862A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- gene
- kit
- synthesis process
- chip
- gene chip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2012100688417A CN102533738B (zh) | 2012-03-15 | 2012-03-15 | 一种基因合成方法、基因芯片及试剂盒 |
PCT/CN2012/074181 WO2013134984A1 (fr) | 2012-03-15 | 2012-04-17 | Procédé de synthèse de gènes, puce à adn et kit |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2826862A1 EP2826862A1 (fr) | 2015-01-21 |
EP2826862A4 true EP2826862A4 (fr) | 2015-10-28 |
Family
ID=46341770
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP12871271.8A Withdrawn EP2826862A4 (fr) | 2012-03-15 | 2012-04-17 | Procédé de synthèse de gènes, puce à adn et kit |
Country Status (4)
Country | Link |
---|---|
US (2) | US9862991B2 (fr) |
EP (1) | EP2826862A4 (fr) |
CN (1) | CN102533738B (fr) |
WO (1) | WO2013134984A1 (fr) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2013302376B2 (en) | 2012-08-16 | 2018-02-22 | Synthetic Genomics, Inc. | Digital to biological converter |
CN103215296B (zh) * | 2013-03-22 | 2014-12-03 | 武汉伯远生物科技有限公司 | 一种多片段dna分子组装方法及应用 |
CN105505755A (zh) * | 2015-12-23 | 2016-04-20 | 杭州谷禾信息技术有限公司 | 空间转录组建库测序方法及所用装置 |
WO2019080704A1 (fr) * | 2017-10-25 | 2019-05-02 | 深圳华大生命科学研究院 | Puce microfluidique pour la synthèse d'acide nucléique |
CN111349673A (zh) * | 2018-12-20 | 2020-06-30 | 江苏金斯瑞生物科技有限公司 | 一种单链dna的合成及错误纠正方法 |
CN113728130A (zh) * | 2019-04-01 | 2021-11-30 | 国立大学法人神户大学 | 嵌合质粒文库的构建方法 |
US20230086471A1 (en) * | 2020-02-15 | 2023-03-23 | Agency For Science, Technology And Research | A method to amplify a nucleic acid |
CN114904469B (zh) * | 2021-02-09 | 2024-05-14 | 深圳华大生命科学研究院 | 合成装置、合成仪及合成方法 |
CN113096731B (zh) * | 2021-03-12 | 2022-01-11 | 云舟生物科技(广州)有限公司 | 载体生产周期的预估方法、计算机存储介质及电子设备 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1257918A (zh) * | 1998-12-18 | 2000-06-28 | 吴光耀 | 重组抗真菌蛋白基因序列、工程菌及其产物制备方法 |
ATE412776T1 (de) * | 2000-11-06 | 2008-11-15 | Ajinomoto Kk | Verfahren zur suche eines genes, welches ein protein des nukleären transportes kodiert |
JP4579525B2 (ja) * | 2003-10-27 | 2010-11-10 | 日立ソフトウエアエンジニアリング株式会社 | 遺伝子発現データ管理表示方法 |
-
2012
- 2012-03-15 CN CN2012100688417A patent/CN102533738B/zh active Active
- 2012-04-17 WO PCT/CN2012/074181 patent/WO2013134984A1/fr active Application Filing
- 2012-04-17 EP EP12871271.8A patent/EP2826862A4/fr not_active Withdrawn
- 2012-04-17 US US14/384,960 patent/US9862991B2/en active Active
-
2017
- 2017-11-29 US US15/825,553 patent/US20180080069A1/en not_active Abandoned
Non-Patent Citations (3)
Title |
---|
I. SAAEM ET AL: "Error correction of microchip synthesized genes using Surveyor nuclease", NUCLEIC ACIDS RESEARCH, vol. 40, no. 3, 29 November 2011 (2011-11-29), pages e23 - e23, XP055212292, ISSN: 0305-1048, DOI: 10.1093/nar/gkr887 * |
MA SIYING ET AL: "Error correction in gene synthesis technology", TRENDS IN BIOTECHNOLOGY, vol. 30, no. 3, 29 November 2011 (2011-11-29), pages 147 - 154, XP028897829, ISSN: 0167-7799, DOI: 10.1016/J.TIBTECH.2011.10.002 * |
See also references of WO2013134984A1 * |
Also Published As
Publication number | Publication date |
---|---|
US20150031088A1 (en) | 2015-01-29 |
US20180080069A1 (en) | 2018-03-22 |
WO2013134984A9 (fr) | 2017-03-09 |
WO2013134984A1 (fr) | 2013-09-19 |
CN102533738B (zh) | 2013-07-31 |
CN102533738A (zh) | 2012-07-04 |
US9862991B2 (en) | 2018-01-09 |
EP2826862A1 (fr) | 2015-01-21 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20140918 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
AX | Request for extension of the european patent |
Extension state: BA ME |
|
DAX | Request for extension of the european patent (deleted) | ||
RA4 | Supplementary search report drawn up and despatched (corrected) |
Effective date: 20150928 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12N 15/10 20060101AFI20150922BHEP Ipc: C40B 40/06 20060101ALI20150922BHEP Ipc: C12M 1/00 20060101ALI20150922BHEP |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20160426 |