EP1651341A2 - Fond d'un reacteur - Google Patents

Fond d'un reacteur

Info

Publication number
EP1651341A2
EP1651341A2 EP04741243A EP04741243A EP1651341A2 EP 1651341 A2 EP1651341 A2 EP 1651341A2 EP 04741243 A EP04741243 A EP 04741243A EP 04741243 A EP04741243 A EP 04741243A EP 1651341 A2 EP1651341 A2 EP 1651341A2
Authority
EP
European Patent Office
Prior art keywords
reactor
closure part
opening
reactor base
collecting funnel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04741243A
Other languages
German (de)
English (en)
Inventor
Alexander Engel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bavarian Nordic AS
Original Assignee
Bavarian Nordic AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bavarian Nordic AS filed Critical Bavarian Nordic AS
Publication of EP1651341A2 publication Critical patent/EP1651341A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/04—Making microcapsules or microballoons by physical processes, e.g. drying, spraying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2/00—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic
    • B01J2/02—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops
    • B01J2/06—Processes or devices for granulating materials, e.g. fertilisers in general; Rendering particulate materials free flowing in general, e.g. making them hydrophobic by dividing the liquid material into drops, e.g. by spraying, and solidifying the drops in a liquid medium

Definitions

  • the invention relates to a reactor base of a reactor, which is used in particular when encapsulating cells, and a method for separating a phase from a phase mixture in a reactor with such a reactor base.
  • DD 217 821 A1 describes the mixing of Langerhans cells with NaCS and the dropping of the mixture into a precipitation bath which contains PDMDAAC.
  • DD 217 821 A1 describes a method for producing a NaCS-PDMDAAC microcapsule system which contains hormone-producing cells.
  • Microcapsules made of NaCS and PDADMAC are also known, inter alia, from DD 160 393 A, DD 219 795 A1, DD 217 821 A1 and DD 274 051 A1.
  • An improved apparatus for the production of NaCS capsules is in Cho (process engineering design of an apparatus for the production of microencapsulated biocatalysts with separate supply of catalyst solution and capsule base substance, progress reports VDI, series 17, no.
  • the reactor or the reactor vessel In a known device for encapsulating cells (Encapsulator AP 'medical' from Inotech), the reactor or the reactor vessel must be removed from the device in order to harvest the capsules. By shaking and tilting the reactor vessel, the capsules are rinsed through a drain opening, which is located about 3 cm above a reactor base in the wall of the reactor vessel, into a collecting vessel or harvesting flask. This procedure is not very user-friendly and leads to an unusable residue of capsules in the reactor vessel.
  • the reactor base of the reactor is formed as a collecting funnel
  • the phase of the phase mixture contained in the reactor or the capsules formed at the bottom or center of the funnel can Meltrichter formed harvest opening and the outlet channel are removed or harvested.
  • a simple handling and operation results from the fact that a closure part is provided for the harvest opening, which is movably arranged on the reactor floor and between a closed position in which it closes the harvest opening of the collecting funnel and a removal position in which it is lowered into the reactor floor and releases a connection between the harvest opening and the outlet channel is adjustable.
  • the harvest opening can be released by adjusting the closure part and the phase to be separated or the capsules can be withdrawn through the outlet channel essentially without residues due to the collecting funnel. Dismantling of the reactor or reactor vessel for manual pouring out or pouring out the phase to be separated or the capsules is not necessary.
  • the surface or top of the closure part is preferably used as a diverting device for diverting the reactor! of? Phase mixture or the capsules formed from the harvest opening in the outlet channel.
  • the Abieit thus supports the almost or completely loss-free draining.
  • the surface or top of the closure part has a collection area with a low level, the collection area being assigned to the outlet channel in the lowered removal position of the closure part.
  • the collection area is in particular part of the drainage device and it can e.g. B. be formed approximately punctiform or linear.
  • a circular or ring-shaped and thus approximately linear collecting area is formed on the periphery of the closure part, for example, in the case of a concavely curved upper side of the closure part.
  • the harvest opening is expediently arranged in the region of the center of the collecting funnel in order to enable the phase or the capsules located above the harvest opening to be removed completely.
  • the crop opening can be arranged both centrally and eccentrically to the central axis or to the center of the collecting funnel, which is formed symmetrically or asymmetrically. It is particularly preferred if the edge of the harvest opening runs through or touches the central axis of the collecting funnel and the surface of the closure part is inclined at the location of the harvest opening in accordance with the inclination of the funnel surface or has the corresponding funnel shape. Due to this shape of the surface, no particles or capsules remain on the lowered closure part, which opens the harvest opening.
  • the surface of the closure part In its closed position, the surface of the closure part is then expediently arranged flush with the surface of the collecting funnel. When the closure part is open, the collecting funnel can be emptied without residue.
  • the surface of the closure part can be beveled or spherical with respect to the central axis, so that no residues of particles or capsules can remain on the surface when they flow or flow out of the reactor ,
  • An outlet recess which extends from the harvest opening in the reactor base and in particular parallel to the central axis of the collecting funnel, is preferably provided, which receives the closure part in a displaceable manner and into which the at least one outlet channel opens.
  • the outlet recess can be a hole with a round cross-section or a non-circular one such as B. elliptical or rectangular cross section.
  • the outlet recess can also run at an acute angle to the central axis.
  • an annular groove and / or a plurality of openings is or are formed in the wall of the outlet recess, the Communicate with the outlet channel, the draining or harvesting of the capsules can be done faster due to the larger drain cross-sections.
  • At least one drain channel covered by a sieve opens into the collecting funnel.
  • a second liquid phase in particular a liquid phase, can be drained or sucked out of the reactor via the discharge channel.
  • a washing or rinsing liquid which has previously been fed to the reactor in order to rinse the capsules or generally the product formed in the reactor can also be drained off via the discharge channel. Such a rinsing process can be carried out once or several times.
  • the reactor base can be formed in one piece with the reactor or the reactor wall. On the other hand, it can be provided that the reactor and the reactor wall to the reactor bottom and is detachably connected to the top of the actuator bottom Re- the Samme! R trichte surrounding reactor wall can be fixed.
  • the reactor floor is made of plastic such as. B. PTFE manufactured.
  • the collecting funnel can also be attached to the reactor base as a separate sheet metal or plastic part.
  • the closure part can be adjustable both manually and by means of a drive device arranged on the reactor base.
  • the funnel angle of the collecting funnel is preferably essentially 130 ° to 170 ° and in particular 153 °. Otherwise, the funnel angle is selected as a function of the capsules or phases to be separated and their flow properties and can therefore have any suitable angle.
  • the phase mixture is introduced into the reactor, the phases are separated and the desired phase is removed by moving the closure part arranged in its closed position into its removal position and thereby releasing a connection between the harvest opening and the outlet channel, via the harvest opening and the outlet channel from the reactor.
  • the phase mixture is a mixture of solid and liquid phases and the phases are separated by sedimentation of the solid phase, capsules in particular forming the solid phase.
  • the phase separation by means of sedimentation is particularly expedient if the phase mixture is a hardening bath with capsules contained in the hardening bath as the phase to be separated and the capsules settle at the bottom of the collecting funnel, where they can be removed.
  • the capsules are, for example, ⁇ aCS capsules and they preferably contain biological cells, in particular animal, human or plant cells.
  • the hardening bath is drained via the drain channel and flushing liquid is fed into the reactor and drained again via the drain channel, this process step being carried out one or more times can.
  • the desired phase which in the reactor z. B. forms a lower layer, can be removed through the harvesting opening and the outlet channel and the middle or the upper layer-like phase can, depending on the arrangement of the phase or layer, through the outlet channel or subsequently hd to the lower phase through the harvest opening and the outlet channel.
  • Figure 1 is a vertical sectional view of a reactor floor according to the invention with an outlet device.
  • FIG. 2 shows a plan view in a reduced representation of the reactor floor shown in FIG. 1;
  • Fig. 3 in a vertical sectional view in an enlarged view an embodiment of the reactor floor with a modified outlet device.
  • a reactor 1 of a device for encapsulating cells, in particular microbial, plant or animal cells, or of biological and chemical substances contains a reactor base 2, which, for. B. is made of a circular or square plate made of PTFE and has on its underside 3 a support device with, for example, four feet 4, which are firmly attached to the underside 3 of the reactor base 2.
  • the support device can be, for example, a support frame on which the reactor base 2 rests.
  • the reactor base 2 is formed on its top 5 as a collecting funnel 7 having a central axis 6, which is surrounded by an edge region 8 of the top 5 and has a funnel angle of, for example, 153 °.
  • the edge area 8 preferably contains a flat shoulder 9 for receiving a sealing ring 10 on which a reactor vessel, for. B. a glass cylinder 11 is arranged liquid-tight.
  • a holding device is preferably attached to the reactor base 2, which, for example, four distributed over the circumference of the reactor base 2 and on the Actuator floor 2 attached rods 12.
  • the rods 12 preferably extend to the upper section of the glass cylinder 11 and fix a reactor head part (not shown) to the glass cylinder 11 by means of a releasable holding device and this to the reactor base 2.
  • the reactor base 2 also contains an outlet or harvest recess 13 which extends downward from the collecting funnel 7 into the reactor base 2 and is formed, for example, as a through-opening which connects the collecting funnel 7 to the underside 3 of the reactor base 2.
  • the z. B. formed as a cylindrical bore outlet recess 13 or through opening is arranged eccentrically and in particular parallel to the central axis 6 that its wall 14, for example, touches the central axis 6 and thus runs through the deepest central point of the collecting funnel 7.
  • a harvest opening 15 is thus formed in the inclined surface of the collecting funnel 7 by the outlet recess 13.
  • a closure member 16 which, for. B.
  • the sealing of the closure part 16 takes place either due to the play-free fit of a plastic such as. B. PTFE manufactured closure member 16 in the outlet recess 13 and / or by a seal (not shown), for. B. an O-ring which is arranged on the circumference of the closure part 16 and bears sealingly on the wall 14 of the outlet recess 13 or alternatively is arranged in an annular groove formed in the wall 14.
  • the closure part 16 is preferably received and guided in the outlet recess 13 in such a way that it cannot twist.
  • This protection against rotation is provided, for example, by a non-round cross-sectional shape of the closure part 16 or the outlet recess 13 or by a corresponding guide which prevents the closure part 16, which is round in cross section, from rotating in the cylindrical outlet recess 13.
  • the top or surface 17 of the closure part 16 is inclined and in particular is formed in accordance with the inclined surface of the collecting funnel 7 adjoining the upper harvest opening 15 of the outlet recess 13, so that the closure part 16 is arranged flush in the surface of the collecting funnel 7 when it is in it upper closed position.
  • the reactor base 2 also contains an outlet channel 18 which, for example, on the one hand in the wall 14 of the outlet recess 13 below the center or on the central axis 6 of the collecting funnel 7 and on the other hand on the underside 3 of the reactor base 2 z. B. opens into an outlet pipe or connector 19.
  • the closure part 16 is preferably movable or displaceable in the outlet recess 13 by means of a drive device.
  • the drive device contains, for example, an electrically attached to the underside 3 of the reactor base plate 2.
  • a manual stroke actuation or a manual displacement of the closure part 16 is provided.
  • the shape or shape of the top or surface 17 of the closure oil 16 forms a drainage device with a collecting area 26 at a low level, so that the reactor contents, e.g. B. a liquid or solids such as capsules or the like, is discharged from the surface 17 in the removal direction or in the direction of the outlet channel 18 substantially without residue.
  • the collecting area 26 extends around the lowest point of the surface 17 on the peripheral edge of the closure part 16.
  • the reactor base 2 also contains an additional drain channel 23, which preferably leads from the surface of the collecting funnel 7 to the underside 3 of the reactor base 2.
  • the outlet recess or through hole is preferably arranged centrally to the central axis 6 of the collecting funnel 7.
  • a groove 25 is preferably formed as an annular channel into which the at least one outlet channel 18 opens.
  • the surface 17 of the closure part 16 is preferably spherical or convex, so that in the lowered removal position of the closure part 16 (shown in broken lines in FIG. 3) the desired phase, in particular capsules formed, can be removed with little loss or without loss.
  • the discharge device here forms an annular collecting area 26, which is lowered on the edge side, on the surface 17 of the closure part 16, in order to B. derive the capsules to the groove 25 or the ring channel.
  • the reactor is e.g. B. used in the encapsulation of cells.
  • a suspension of sodium cellulose sulphate and cells is dripped through a nozzle. The drops fall into a hardness bath made of PDADMAC contained in the reactor 1 or the reactor vessel 11.
  • the closure part 16 is arranged in its upper closing division flush with the surface of the collecting funnel 7 and the drain channel 23 is blocked off.
  • the hardening bath is drained or pumped out via the drain channel 23, the sieve 24 holding back the encapsulated substances or capsules.
  • a rinsing or washing liquid can then be filled into the reactor and, after washing the capsules, drained off via the drain channel 23. This washing process can be carried out once or several times.
  • the closure member 16 is moved downward until the opening of the outlet channel 18 in the wall 14 of the outlet recess 13 is exposed and thus the capsules can flow through the harvest opening 15 and the outlet channel 18 with little loss or loss-free, since no corners are present on the flow path in which capsules may remain.
  • the upper side or surface 17 of the closure part 16 enables and thus supports the loss-free drainage of the capsules from the reactor 1.
  • the reactor 1 with the described reactor bottom can be used very generally to separate a phase from a phase mixture with at least two phases, the phase located above the surface of the collecting funnel 7 through the harvest opening 15 with little loss or can even be deducted without loss.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Mixers Of The Rotary Stirring Type (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Manufacturing Of Micro-Capsules (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)

Abstract

Fond (2) d'un réacteur (1) qui comporte une face supérieure (5) conçue sous forme trémie collectrice (7), un orifice d'évacuation (15) formé dans la trémie collectrice (7) et raccordé à une conduite d'évacuation (18) traversant le fond (2) du réacteur et un élément de fermeture (16) de l'orifice d'évacuation (15) placé mobile dans le fond (2) du réacteur et pouvant être déplacé entre une position de fermeture dans laquelle il ferme l'orifice d'évacuation (15) de la trémie collectrice (7) et une position de prélèvement dans laquelle il est enfoncé dans le fond (2) du réacteur et libère une liaison entre l'orifice d'évacuation (15) et la conduite d'évacuation (18). La présente invention concerne en outre un procédé de séparation d'une phase ou de capsules d'un mélange de phases ou d'une solution de durcissement, en particulier au moyen de la sédimentation, dans un réacteur comportant un fond de ce type.
EP04741243A 2003-07-25 2004-07-23 Fond d'un reacteur Withdrawn EP1651341A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10334371A DE10334371A1 (de) 2003-07-25 2003-07-25 Reaktorboden eines Reaktors
PCT/EP2004/008260 WO2005011857A2 (fr) 2003-07-25 2004-07-23 Fond d'un reacteur

Publications (1)

Publication Number Publication Date
EP1651341A2 true EP1651341A2 (fr) 2006-05-03

Family

ID=34071952

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04741243A Withdrawn EP1651341A2 (fr) 2003-07-25 2004-07-23 Fond d'un reacteur

Country Status (6)

Country Link
US (1) US20060207682A1 (fr)
EP (1) EP1651341A2 (fr)
JP (1) JP2006528545A (fr)
CA (1) CA2533487A1 (fr)
DE (1) DE10334371A1 (fr)
WO (1) WO2005011857A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3501550A1 (fr) 2012-04-02 2019-06-26 Moderna Therapeutics, Inc. Polynucléotides modifiés pour la production de protéines associées à une maladie humaine
DE102016014457A1 (de) * 2016-12-05 2018-06-07 Pva Tepla Ag Probengefäß zur Aufnahme kleinvolumiger flüssiger Proben

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD38062A (fr) *
GB685371A (en) * 1949-06-07 1953-01-07 North Thames Gas Board Means embodying a gas-tight valve for use in removing flowable solids from or introducing flowable solids into gas-solid reaction vessels
US3690842A (en) * 1970-10-05 1972-09-12 Universal Oil Prod Co Reactor for fluidized particles with adjustable level weir to particle outlet
DD217821A1 (de) * 1983-08-08 1985-01-23 Adw Inst Polymerenchemie Verfahren zur herstellung eines hormonproduzierenden mikrokapselsystems
JPS6251957A (ja) * 1985-08-30 1987-03-06 Hitachi Ltd 海藻ゲル包括菌体粒子ゲル製造装置及び方法
JPS63248381A (ja) * 1987-04-03 1988-10-14 Hitachi Ltd 無菌式吐出弁
DE4316944C2 (de) * 1993-05-21 2000-10-12 Puth Hans Edgar Verfahren und Vorrichtung zur semikontinuierlichen Herstellung von Milchsäure aus Würze
US5733776A (en) * 1993-11-09 1998-03-31 Genzyme Corporation Continuous settling apparatus
US5925563A (en) * 1997-07-21 1999-07-20 Redford; Steven G. Multi-stage column continuous fermentation system
JP3329709B2 (ja) * 1997-10-27 2002-09-30 株式会社総合開発 反応器
IT1307234B1 (it) * 1999-04-01 2001-10-30 Gimar Tecno S R L Vasca di fermentazione, particolarmente per la vinificazione in rosso.
DE19923628C2 (de) * 1999-05-22 2002-03-07 Herbert Huettlin Verfahren und Vorrichtung zum Herstellen von partikelförmigen Teilchen
US6780507B2 (en) * 2000-02-09 2004-08-24 Analytical Research Systems, Inc. Hydrocapsules and method of preparation thereof
DE10024154A1 (de) * 2000-05-19 2001-11-22 Inotech Encapsulation Ag Dotti Verfahren und Vorrichtung zur Verkapselung von Zellen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2005011857A3 *

Also Published As

Publication number Publication date
WO2005011857A3 (fr) 2005-04-07
WO2005011857A2 (fr) 2005-02-10
US20060207682A1 (en) 2006-09-21
DE10334371A1 (de) 2005-02-17
JP2006528545A (ja) 2006-12-21
CA2533487A1 (fr) 2005-02-10

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