EP1046073A1 - Optische anordnung im beleuchtungsstrahlengang eines mikroskops - Google Patents
Optische anordnung im beleuchtungsstrahlengang eines mikroskopsInfo
- Publication number
- EP1046073A1 EP1046073A1 EP99907238A EP99907238A EP1046073A1 EP 1046073 A1 EP1046073 A1 EP 1046073A1 EP 99907238 A EP99907238 A EP 99907238A EP 99907238 A EP99907238 A EP 99907238A EP 1046073 A1 EP1046073 A1 EP 1046073A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- optics
- arrangement according
- illumination
- beam path
- designed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
Definitions
- the invention relates to an optical arrangement in the illumination beam path of a microscope, in particular a confocal laser microscope.
- the actually gaussian laser beam is expanded in the illuminating beam path by means of a suitable optical system in such a way that the entrance pupil of the respective objective or of the lenses that can be used there is fundamentally over-illuminated.
- the degree of over-lighting is an important design requirement.
- the overexposure of the entrance pupil is used for homogeneous illumination of the entrance pupil, the theoretical resolution having to be ensured in particular also with lenses with different apertures.
- the overexposure of the entrance pupil leads to considerable losses of the excitation light, particularly in the case of objectives with a small entrance pupil. However, such losses of excitation light are unacceptable in applications with low power reserves in the excitation light.
- the Leica TCS laser scanning microscope is known from practice, in which a fixed expansion lens is provided.
- the diameter of the expanded laser beam there is about 25 mm (1 / e 2 value) on the microscope objective.
- a PL APO 40x / 1, 25 objective has an entrance pupil of approximately 12 mm in diameter.
- a PL APO 100x / 1, 4 lens has an entrance pupil of only 5 mm in diameter.
- the pinhole transmission is characterized in proportion to the area of the pinhole by corresponding light losses with small diameters. This is also not acceptable in practice.
- the invention is based on the object of specifying an optical arrangement in the illumination beam path of a microscope, in which optimal illumination is ensured while reducing losses of the excitation light.
- optical arrangement of the generic type according to the invention achieves the above object by the features of claim 1. According to this, such an optical arrangement is characterized by an illumination optics arranged in the illumination beam path for changing the illumination diameter.
- the illuminating diameter of the illuminating beam path should be adapted more or less exactly to the entrance pupil of the respective objective in order to avoid light losses.
- This requires an illumination optics arranged in the illumination beam path, with the aid of which the change or adaptation of the illumination diameter can take place. Loss of light according to the In any case, the prior art discussed above can be avoided as far as possible.
- the lighting optics provided according to the invention could be designed as an arrangement of interchangeable fixed optics.
- Illumination beam path are exchanged so that the illumination diameter is matched to the entrance pupil of the respective lens.
- the illumination optics comprise a preferably continuously variable vario optics, so that the exchange of fixed optics in the illumination beam path is not necessary.
- the vario optics can be a preferably motorized zoom optics, which in turn can be designed as conventional zoom optics, as is used, for example, in commercially available video cameras.
- An automatic setting could be provided for the simple and optimal adaptation of the illumination diameter to the entrance pupils of several lenses.
- the change in the illumination diameter could be coordinated with the entrance pupils of predetermined lenses, preferably arranged in a revolver, the change or adaptation taking place automatically depending on the use of the respective objective, corresponding to the position in the revolver.
- the lighting optics prefferably be arranged downstream of a point light source or an optical fiber.
- the lighting optics could be more variable as parallelization optics with a fixed focal length
- Focal length can be designed, wherein the beam diameter of the entrance pupil of the lens is adjustable. It is also conceivable that the illumination optics are designed as expansion optics for a preferably directly coupled laser beam. In this respect, the beam could be variably expandable according to the ratio of the focal lengths f-
- the illumination optics comprise a further optical component which influences or promotes the edge illumination, the over-illumination known from the prior art being avoided in any case .
- Such an optical component could be designed as an additional lens, as a ring diaphragm or as a holographically produced optical element, whereby the usually Gaussian laser beam in particular is expanded in the edge regions.
- an additional input is provided for feeding in a further light source, which is preferably the coupling of a laser light beam.
- this laser light beam could be adaptable to the entrance pupil of the objective, which also makes it possible to optimize the laser light beam without adapting the actual illuminating beam path.
- an arrangement of the type mentioned above could advantageously be used in multi-photon laser scanning microscopy or for multi-photon excitation.
- FIG. 1 shows a schematic representation of a first exemplary embodiment of an optical arrangement according to the invention in the beam path of a confocal scanning microscope, a point light source being provided as the light source and the scanning microscope being shown only schematically by its components for the sake of simplicity;
- FIG. 2 shows a schematic representation of a second exemplary embodiment of an optical arrangement according to the invention in the beam path of a confocal scanning microscope, an optical fiber being provided as the light source and the scanning microscope being shown only schematically by its components for the sake of simplicity and
- FIG. 3 shows a schematic illustration of a third exemplary embodiment of an optical arrangement according to the invention in the beam path of a confocal scanning microscope, a laser light source or a laser beam and the scanning microscope being the light source
- 1 to 3 each show an optical arrangement in the illumination beam path 1 of a confocal scanning microscope, the scanning microscope as a whole being shown only schematically for the sake of simplicity.
- a point light source 2 is symbolically shown as the light source in FIG. 1, the light is coupled in via an optical fiber 3 in FIG.
- a laser beam 4 or a parallel light beam from an alternative / conventional light source is coupled directly into the illumination beam path 1 via a lens 5.
- an illumination optics 6 is arranged in the illumination beam path 1. This illumination optics 6 serves to change the illumination diameter 7, so that an adaptation of the illumination diameter 7 to the symbolically represented entrance pupil 8 of the objective 9 is possible.
- Illumination beam path 1 up to the object 10, but rather also a beam combiner 11 arranged in the illumination beam path 1 and a scanner 12.
- a pinhole optics 14 and a detection pinhole 15 are arranged schematically in the detection beam path 13.
- the illumination optics 6 are designed as continuously variable vario optics. More precisely, this is a motorized zoom optic, which is, however, only shown symbolically by a displaceable lens 16. Specifically, this is a conventional zoom optic, as is known from video cameras. In the embodiment shown in FIG. 3, the lighting optics 6 are preceded by a lens 5, into which the laser beam 4 couples directly.
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19801833 | 1998-01-14 | ||
DE19801833 | 1998-01-14 | ||
PCT/DE1999/000062 WO1999036822A1 (de) | 1998-01-14 | 1999-01-14 | Optische anordnung im beleuchtungsstrahlengang eines mikroskops |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1046073A1 true EP1046073A1 (de) | 2000-10-25 |
EP1046073B1 EP1046073B1 (de) | 2015-04-08 |
Family
ID=7855046
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99907238.2A Revoked EP1046073B1 (de) | 1998-01-14 | 1999-01-14 | Verfahren zur abstimmung des beleuchtungsstrahlengangs eines konfokalen mikroskops auf die eintrittspupille eines objektivs |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1046073B1 (de) |
JP (1) | JP2002509287A (de) |
DE (1) | DE19901219B4 (de) |
WO (1) | WO1999036822A1 (de) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10139920B4 (de) | 2001-08-14 | 2008-07-31 | Leica Microsystems Cms Gmbh | Scanmikroskop und Verfahren zum Scannen eines Objekts |
DE102004034966A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Beleuchtungsvorrichtung für ein Lichtrastermikroskop mit linienförmiger Abtastung und Verwendung |
DE102004034967A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Beleuchtungsvorrichtung für ein Lichtrastermikroskop mit punktförmiger Lichtquellenverteilung |
DE102004034990A1 (de) | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Zoomoptik für ein Lichtrastermikroskop mit linienförmiger Abtastung und Verwendung |
DE102004034991A1 (de) | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Zoomoptik für ein Lichtrastermikroskop |
DE102004034989A1 (de) * | 2004-07-16 | 2006-02-09 | Carl Zeiss Jena Gmbh | Zoomoptik für ein Lichtrastermikroskop mit punktförmiger Lichtquellenverteilung und Verwendung |
DE102005034441A1 (de) | 2005-07-22 | 2007-02-22 | Carl Zeiss Microimaging Gmbh | Mikroskopobjektiv |
DE102005034442A1 (de) | 2005-07-22 | 2007-02-22 | Carl Zeiss Microimaging Gmbh | Mikroskopobjektivsystem |
JP2009080502A (ja) * | 2008-12-05 | 2009-04-16 | Carl Zeiss Jena Gmbh | 走査ユニット付き顕微鏡における放射を走査ヘッドに結合するための配置およびその操作方法 |
JP5616824B2 (ja) | 2011-03-10 | 2014-10-29 | オリンパス株式会社 | 顕微鏡装置 |
DE102011087196A1 (de) | 2011-11-28 | 2013-05-29 | Leica Microsystems Cms Gmbh | Mikroskopbeleuchtungssystem und -verfahren |
Family Cites Families (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3679287A (en) * | 1969-12-16 | 1972-07-25 | Nippon Kogaku Kk | Illumination system for light microscopes |
JPS49129547U (de) | 1973-03-05 | 1974-11-07 | ||
US3876289A (en) | 1973-03-26 | 1975-04-08 | American Optical Corp | Illumination zoom system for microscopes |
GB1595422A (en) | 1977-04-28 | 1981-08-12 | Nat Res Dev | Scaning microscopes |
JPS56165111A (en) | 1980-05-26 | 1981-12-18 | Nippon Kogaku Kk <Nikon> | Telecentric illuminating system |
JPS575024A (en) | 1980-06-13 | 1982-01-11 | Olympus Optical Co Ltd | Lighting changer for microscope |
DE3221804A1 (de) * | 1982-06-07 | 1983-12-08 | Leisegang Feinmechanik-Optik Gmbh & Co Kg, 1000 Berlin | Beleuchtungseinrichtung |
JPS59211014A (ja) | 1983-05-16 | 1984-11-29 | Nippon Kogaku Kk <Nikon> | 変倍観察装置 |
US4827125A (en) * | 1987-04-29 | 1989-05-02 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Confocal scanning laser microscope having no moving parts |
JP2855271B2 (ja) | 1987-06-30 | 1999-02-10 | 株式会社トプコン | 照明・観察光学装置 |
JPH06100724B2 (ja) | 1988-07-04 | 1994-12-12 | 大日本スクリーン製造株式会社 | 顕微鏡の照明光学系 |
JP2959830B2 (ja) | 1990-10-30 | 1999-10-06 | オリンパス光学工業株式会社 | 光走査装置 |
DE4301716C2 (de) * | 1992-02-04 | 1999-08-12 | Hitachi Ltd | Projektionsbelichtungsgerät und -verfahren |
JPH05323195A (ja) | 1992-05-15 | 1993-12-07 | Casio Comput Co Ltd | ズームレンズ |
DE4231406A1 (de) * | 1992-09-19 | 1994-03-24 | Leica Mikroskopie & Syst | Hellfeld-Durchlicht-Beleuchtungseinrichtung für Mikroskope |
JPH0815156A (ja) | 1993-06-03 | 1996-01-19 | Hamamatsu Photonics Kk | レーザスキャン光学系及びレーザスキャン光学装置 |
DE4404283A1 (de) | 1994-02-11 | 1995-08-17 | Leica Mikroskopie & Syst | Kondensorsystem für Mikroskope |
JPH07234382A (ja) | 1994-02-24 | 1995-09-05 | Matsushita Electric Ind Co Ltd | 超解像走査光学装置 |
DE4423705A1 (de) * | 1994-06-24 | 1996-01-04 | Frankowski Gottfried Dr | Strukturierte Leuchtfeldblende |
CH693804A5 (de) * | 1994-10-13 | 2004-02-13 | Zeiss Carl Fa | Beleuchtungseinrichtung für ein Stereomikroskop. |
JPH09318879A (ja) | 1996-05-29 | 1997-12-12 | Komatsu Ltd | 共焦点光学装置 |
DE19654211C2 (de) * | 1996-12-24 | 2000-07-06 | Leica Microsystems | Konfokales Mikroskop |
-
1999
- 1999-01-14 EP EP99907238.2A patent/EP1046073B1/de not_active Revoked
- 1999-01-14 WO PCT/DE1999/000062 patent/WO1999036822A1/de active Application Filing
- 1999-01-14 DE DE19901219.9A patent/DE19901219B4/de not_active Expired - Lifetime
- 1999-01-14 JP JP2000540475A patent/JP2002509287A/ja active Pending
Non-Patent Citations (1)
Title |
---|
See references of WO9936822A1 * |
Also Published As
Publication number | Publication date |
---|---|
DE19901219B4 (de) | 2017-10-19 |
WO1999036822A1 (de) | 1999-07-22 |
JP2002509287A (ja) | 2002-03-26 |
EP1046073B1 (de) | 2015-04-08 |
DE19901219A1 (de) | 1999-09-09 |
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