DK496489A - Fremgangsmaade til stabilisering af proteiner ved tilrettelaeggelse af indbyrdes elektrostatiske paavirkninger ved metalionbindingssteder - Google Patents

Fremgangsmaade til stabilisering af proteiner ved tilrettelaeggelse af indbyrdes elektrostatiske paavirkninger ved metalionbindingssteder Download PDF

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Publication number
DK496489A
DK496489A DK496489A DK496489A DK496489A DK 496489 A DK496489 A DK 496489A DK 496489 A DK496489 A DK 496489A DK 496489 A DK496489 A DK 496489A DK 496489 A DK496489 A DK 496489A
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DK
Denmark
Prior art keywords
metalion
organization
procedure
electrostatic effects
stabilizing proteins
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DK496489A
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English (en)
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DK496489D0 (da
DK175917B1 (da
Inventor
Michael W Pantoliano
Barry C Finzel
Philip N Bryan
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Genex Corp
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21879773&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK496489(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Laminated Bodies (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DK198904964A 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet DK175917B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06
US3496587 1987-04-06
PCT/US1988/001235 WO1988008028A1 (en) 1987-04-06 1988-04-06 The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US8801235 1988-04-06

Publications (3)

Publication Number Publication Date
DK496489D0 DK496489D0 (da) 1989-10-06
DK496489A true DK496489A (da) 1989-10-06
DK175917B1 DK175917B1 (da) 2005-06-27

Family

ID=21879773

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198904964A DK175917B1 (da) 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet

Country Status (6)

Country Link
EP (2) EP0353250B1 (da)
JP (2) JP3155984B2 (da)
AT (2) ATE184316T1 (da)
DE (3) DE3856362T2 (da)
DK (1) DK175917B1 (da)
WO (1) WO1988008028A1 (da)

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US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
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DE69133632D1 (de) * 1990-01-04 2010-05-27 Danisco Us Inc Glukose-Isomerasen mit geänderter Substratspezifität
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US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
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CN1554750B (zh) 1996-11-04 2011-05-18 诺维信公司 枯草酶变种及组合物
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EP1012251A1 (en) 1997-08-29 2000-06-28 Novo Nordisk A/S Protease variants and compositions
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US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
KR20010052223A (ko) * 1998-03-26 2001-06-25 데이비드 엠 모이어 아미노산 결실 및 치환을 갖는 세린 프로테아제 변이체
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WO2000071687A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
EP1183340B1 (en) 1999-05-20 2008-09-03 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
ATE408680T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
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EP1206526A2 (en) 1999-07-22 2002-05-22 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
KR20020021395A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 입체적으로 보호된 ｃｌｉｐ 부위를 갖는 프로테아제콘쥬게이트
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
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US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
GB0013643D0 (en) 2000-05-31 2000-07-26 Unilever Plc Targeted moieties for use in bleach catalysts
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
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DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
EP2325318A1 (en) * 2004-02-13 2011-05-25 Novozymes A/S Protease variants
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
EP1598367A1 (en) * 2004-05-18 2005-11-23 ZLB Behring GmbH Modified coagulation factors with enhanced stability and their derivatives
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EP2013339B1 (en) 2006-04-20 2013-09-11 Novozymes A/S Savinase variants having an improved wash performance on egg stains
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DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
WO2012022777A1 (en) 2010-08-19 2012-02-23 Novozymes A/S Induced sporulation screening method
BR112013033816A2 (pt) 2011-07-01 2017-02-14 Novozymes A / S composição de detergente líquido sem boro
EP2970830B1 (en) 2013-03-14 2017-12-13 Novozymes A/S Enzyme and inhibitor contained in water-soluble films
EP3461881A1 (en) 2013-05-03 2019-04-03 Novozymes A/S Microencapsulation of detergent enzymes
WO2017117089A1 (en) 2015-12-28 2017-07-06 Novozymes Bioag A/S Heat priming of bacterial spores
JP7317811B2 (ja) 2017-09-27 2023-07-31 ノボザイムス アクティーゼルスカブ リパーゼ変異体及びかかるリパーゼ変異体を含むマイクロカプセル組成物
CN111801416A (zh) 2018-02-08 2020-10-20 诺维信公司 脂肪酶变体及其组合物
CN111868239A (zh) 2018-02-08 2020-10-30 诺维信公司 脂肪酶、脂肪酶变体及其组合物
CN111770788B (zh) 2018-03-13 2023-07-25 诺维信公司 使用氨基糖低聚物进行微囊化
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
CN114207123A (zh) 2019-07-02 2022-03-18 诺维信公司 脂肪酶变体及其组合物
JP7425459B2 (ja) * 2019-10-11 2024-01-31 株式会社シノテスト 安定化されたｈｍｇｂ１含有溶液
KR20230057391A (ko) 2020-08-24 2023-04-28 노보자임스 에이/에스 프럭타나제를 포함하는 구강 관리 조성물

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WO1990014420A1 (en) * 1989-05-17 1990-11-29 Amgen Inc. Multiply mutated subtilisins

Also Published As

Publication number Publication date
ATE184316T1 (de) 1999-09-15
ATE371029T1 (de) 2007-09-15
JP2001029076A (ja) 2001-02-06
DE3856362T2 (de) 2000-05-18
DE3856593D1 (de) 2007-10-04
EP0916732B1 (en) 2007-08-22
EP0353250B1 (en) 1999-09-08
DE353250T1 (de) 1998-05-28
EP0353250A4 (en) 1990-09-05
JPH02502874A (ja) 1990-09-13
EP0353250A1 (en) 1990-02-07
DK496489D0 (da) 1989-10-06
WO1988008028A1 (en) 1988-10-20
EP0916732A1 (en) 1999-05-19
DK175917B1 (da) 2005-06-27
DE3856362D1 (de) 1999-10-14
JP3155984B2 (ja) 2001-04-16

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