DK3286300T3 - Isolering af bona fide pankreatiske stamceller - Google Patents

Isolering af bona fide pankreatiske stamceller Download PDF

Info

Publication number
DK3286300T3
DK3286300T3 DK16721712.4T DK16721712T DK3286300T3 DK 3286300 T3 DK3286300 T3 DK 3286300T3 DK 16721712 T DK16721712 T DK 16721712T DK 3286300 T3 DK3286300 T3 DK 3286300T3
Authority
DK
Denmark
Prior art keywords
isolation
stem cells
bona fide
pancreatic stem
fide pancreatic
Prior art date
Application number
DK16721712.4T
Other languages
English (en)
Inventor
Henrik Semb
Jacqueline Ameri
Original Assignee
Univ Copenhagen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Copenhagen filed Critical Univ Copenhagen
Application granted granted Critical
Publication of DK3286300T3 publication Critical patent/DK3286300T3/da

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/58—Adhesion molecules, e.g. ICAM, VCAM, CD18 (ligand), CD11 (ligand), CD49 (ligand)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/585—Integrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
DK16721712.4T 2015-04-24 2016-04-21 Isolering af bona fide pankreatiske stamceller DK3286300T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15164999 2015-04-24
PCT/EP2016/058920 WO2016170069A1 (en) 2015-04-24 2016-04-21 Isolation of bona fide pancreatic progenitor cells

Publications (1)

Publication Number Publication Date
DK3286300T3 true DK3286300T3 (da) 2021-01-18

Family

ID=53016496

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16721712.4T DK3286300T3 (da) 2015-04-24 2016-04-21 Isolering af bona fide pankreatiske stamceller

Country Status (12)

Country Link
US (3) US10494608B2 (da)
EP (2) EP3819372A3 (da)
JP (1) JP6954711B2 (da)
CN (1) CN107787363B (da)
CA (1) CA2983764A1 (da)
CY (1) CY1123677T1 (da)
DK (1) DK3286300T3 (da)
ES (1) ES2840076T3 (da)
HK (1) HK1248277A1 (da)
PL (1) PL3286300T3 (da)
PT (1) PT3286300T (da)
WO (1) WO2016170069A1 (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107787363B (zh) 2015-04-24 2021-06-25 哥本哈根大学 真正的胰腺祖细胞的分离
EP3638774A1 (en) 2017-06-14 2020-04-22 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
CA3101021A1 (en) * 2018-05-31 2019-12-05 University Health Network Methods and compositions comprising tankyrase inhibitors for generating insulin producing cells
WO2020068984A1 (en) * 2018-09-25 2020-04-02 The Regents Of The University Of California Production and enrichment of pancreatic endocrine progenitor cells
JP7389980B2 (ja) * 2018-12-06 2023-12-01 国立大学法人 琉球大学 ヒト膵臓組織特異的幹／前駆細胞の人工作製方法
WO2021051256A1 (en) * 2019-09-17 2021-03-25 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Pancreatic pro-endocrine progenitor cells and use thereof
US20230128770A1 (en) * 2019-10-21 2023-04-27 President And Fellows Of Harvard College Compositions and methods for enhancing differentiation of stem cell-derived beta cells

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005512593A (ja) 2001-12-28 2005-05-12 セルアーティス アーベー 多能性のヒト胚盤胞由来幹細胞株の樹立方法
JPWO2003087349A1 (ja) * 2002-04-17 2005-08-18 大塚製薬株式会社 間葉系細胞から膵β細胞を形成する方法
WO2004010933A2 (en) 2002-07-25 2004-02-05 The Board Of Trustees Of The Leland Stanford Junoir University Insulin-producing cell compositions and related methods
US20050196784A1 (en) * 2002-11-01 2005-09-08 Decode Genetics Ehf. Human Type II diabetes gene - Kv channel-interacting protein (KChIP1) located on chromosome 5
US20070154981A1 (en) 2003-02-14 2007-07-05 The Board Of Trustees Of The Leland Stanford Junior University Insulin-producing cells derived from stem cells
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US7704738B2 (en) 2003-12-23 2010-04-27 Cythera, Inc. Definitive endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
JP4926946B2 (ja) 2004-04-27 2012-05-09 ヴィアサイト，インコーポレイテッド Ｐｄｘ１発現性内胚葉
US20060153894A1 (en) 2004-06-30 2006-07-13 Ragae Ghabrial Multi-compartment delivery system
AU2006210955A1 (en) 2005-01-31 2006-08-10 Es Cell International Pte Ltd. Directed differentiation of embryonic stem cells and uses thereof
ES2627419T3 (es) 2005-03-04 2017-07-28 Lifescan, Inc. Células estromales adultas derivadas del páncreas
US20100129906A1 (en) 2005-10-07 2010-05-27 Cellartis Ab Method for Obtaining Xeno-Free Hbs Cell line
WO2007103282A2 (en) 2006-03-02 2007-09-13 Cythera, Inc. Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
CA2650812C (en) 2006-04-28 2017-12-12 Lifescan, Inc. Differentiation of human embryonic stem cells
US20070259423A1 (en) * 2006-05-02 2007-11-08 Jon Odorico Method of differentiating stem cells into cells of the endoderm and pancreatic lineage
AU2007277364B2 (en) 2006-07-26 2010-08-12 Viacyte, Inc. Methods of producing pancreatic hormones
CN103627671A (zh) 2007-01-30 2014-03-12 佐治亚大学研究基金会 产生中内胚层细胞及多能游走细胞的方法与细胞群及用途
EP3957716A1 (en) 2007-07-18 2022-02-23 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
EP2185693B1 (en) 2007-07-31 2019-07-03 Lifescan, Inc. Differentiation of human embryonic stem cells
EP2227487A1 (en) 2007-12-28 2010-09-15 Novo Nordisk A/S Ddr1-mediated cell purification of pancreatic endocrine cells through their progenitors
JP2011518547A (ja) 2008-04-03 2011-06-30 ノボ・ノルデイスク・エー／エス 膵臓内分泌プレ前駆体細胞及びその子孫のｄｎｅｒ媒介細胞精製
WO2009126927A2 (en) 2008-04-11 2009-10-15 Joslin Diabetes Center, Inc. Methods of generating insulin-producing cells
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
DK2283117T3 (da) 2008-04-21 2014-01-20 Viacyte Inc Fremgangsmåde til oprensning af pancreatiske endodermceller afledt fra humane embryoniske stamceller
US7939322B2 (en) 2008-04-24 2011-05-10 Centocor Ortho Biotech Inc. Cells expressing pluripotency markers and expressing markers characteristic of the definitive endoderm
EP2297298A4 (en) * 2008-05-09 2011-10-05 Vistagen Therapeutics Inc PANCREATIC ENDOCRINE PROGENITOR CELLS FROM PLURIPOTENT STEM CELLS
TWI438275B (zh) 2008-06-11 2014-05-21 Healthbanks Biotech Co Ltd 促進幹細胞分化為胰島素製造細胞的方法
CA2742583C (en) 2008-11-04 2022-09-27 Viacyte, Inc. Stem cell aggregate suspension compositions and methods for differentiation thereof
JP2012508584A (ja) 2008-11-14 2012-04-12 ヴィアサイト，インコーポレイテッド ヒト多能性幹細胞由来膵臓細胞のカプセル化
DE102008063951A1 (de) 2008-12-19 2010-06-24 Leopold Kostal Gmbh & Co. Kg Winkelsensor
WO2011128897A1 (en) 2010-04-12 2011-10-20 Technion Research & Development Foundation Ltd. Populations of pancreatic progenitor cells and methods of isolating and using same
US10000739B2 (en) 2010-10-28 2018-06-19 National University Corporation Kumamoto University Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency
WO2012070014A2 (en) * 2010-11-26 2012-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells
WO2013086008A1 (en) 2011-12-05 2013-06-13 Factor Bioscience Inc. Methods and products for transfecting cells
WO2013106844A2 (en) * 2012-01-13 2013-07-18 Oncocyte Corporation Methods and compositions for the treatment and diaginosis of pancreatic cancer
PT2844739T (pt) 2012-04-30 2019-10-01 Univ Health Network Métodos e composições para gerar progenitores pancreáticos e células beta funcionais a partir de hpscs
WO2013188138A1 (en) 2012-06-11 2013-12-19 The Regents Of The University Of California Inhibitors of hippo-yap signaling pathway
US20140030234A1 (en) 2012-07-12 2014-01-30 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for modulating islet beta cell development
ES2813407T3 (es) 2012-08-06 2021-03-23 Brainstorm Cell Therapeutics Ltd Métodos para generar células madre mesenquimales que secretan factores neurotróficos
WO2014127219A1 (en) 2013-02-14 2014-08-21 The Cleveland Clinic Foundation Methods for induction of cell fates from pluripotent cells
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
US20140329704A1 (en) 2013-03-28 2014-11-06 President And Fellows Of Harvard College Markers for mature beta-cells and methods of using the same
DE202014011287U1 (de) 2013-06-11 2019-02-06 The President And Fellows Of Harvard College SC-ß Zellen und Zusammensetzungen zur Erzeugung der Zellen
JP2017521089A (ja) 2014-05-14 2017-08-03 ザ ユニバーシティー コート オブ ザ ユニバーシティー オブ アバーディーンＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｃｏｕｒｔ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ａｂｅｒｄｅｅｎ 膵内分泌細胞の入手方法
CA2948751A1 (en) 2014-05-14 2015-11-19 The University Court Of The University Of Aberdeen Methods of obtaining islet cells
US10920199B2 (en) 2015-02-27 2021-02-16 Salk Institute For Biological Studies Reprogramming progenitor compositions and methods of use therefore
CN107787363B (zh) 2015-04-24 2021-06-25 哥本哈根大学 真正的胰腺祖细胞的分离
US20190390169A1 (en) 2017-03-03 2019-12-26 Kyoto University Pancreatic progenitor cell production method
EP3638774A1 (en) 2017-06-14 2020-04-22 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US20190128885A1 (en) 2017-07-25 2019-05-02 University Health Network Selection of pancreatic progenitors

Also Published As

Publication number Publication date
EP3819372A3 (en) 2021-10-06
EP3286300B1 (en) 2020-10-21
HK1248277A1 (zh) 2018-10-12
PL3286300T3 (pl) 2021-05-04
US11613736B2 (en) 2023-03-28
ES2840076T3 (es) 2021-07-06
CN107787363A (zh) 2018-03-09
JP6954711B2 (ja) 2021-10-27
PT3286300T (pt) 2020-12-28
EP3819372A2 (en) 2021-05-12
US20180127724A1 (en) 2018-05-10
WO2016170069A1 (en) 2016-10-27
CA2983764A1 (en) 2016-10-27
US10494608B2 (en) 2019-12-03
CN107787363B (zh) 2021-06-25
US20200080062A1 (en) 2020-03-12
US20230227788A1 (en) 2023-07-20
CY1123677T1 (el) 2022-03-24
EP3286300A1 (en) 2018-02-28
JP2018512886A (ja) 2018-05-24

Similar Documents

Publication Publication Date Title
DK3445838T3 (da) Isolering af planteekstrakter
DK3371171T3 (da) Inhibitorer af RET
DK3468573T3 (da) Behandling af clostridium difficile infektion
DK3192902T3 (da) Fremgangsmåde til fremstilling af graphenholdige viscosefibre
DK3554240T3 (da) Kombinationer af aktive forbindelser
DK3096741T3 (da) Fremgangsmåde til fremstillingen af hybridosomer
DK3137169T3 (da) Hæmmere af lysin-specifik demethylase-1
DK3325443T3 (da) Fremgangsmåde til fremstilling af pridopidin
DK3259349T3 (da) Dehydrogenase-katalyseret fremstilling af FDCA
DK3317400T3 (da) Fremgangsmåde til kryokonservering af tumorinfiltrerende lymfocytter
DK3286300T3 (da) Isolering af bona fide pankreatiske stamceller
DK3174858T3 (da) Fremgangsmåde til fremstilling af pyrazoler
DK3466432T3 (da) Fremgangsmåder til behandling af pulmonale non-tuberkuløse mykobakterielle infektioner
DK3461891T3 (da) Nedstrømsbehandling af en alkalisk phosphatase
DK3668851T3 (da) Fremgangsmåde til fremstilling af thiocarbonater
DK3390390T3 (da) Bipyrazolylderivater egnede til behandling af autoimmunsygdomme
DK3349772T3 (da) Fremgangsmåde til fremstilling af urteekstrakter
DK3389692T3 (da) Modulatorer af komplement-aktivitet
DK3689380T3 (da) Bløde kapsler af calcifediol
DK3283210T3 (da) Fremgangsmåde
DK3307267T3 (da) Behandling af multipel sklerose
DK3280408T3 (da) Fremgangsmåde til fremstilling af dicycloplatin
DK3363148T3 (da) Afdækning af krypteringsanvendelse
DK3262023T3 (da) Fremgangsmåde til fremstillingen af diener
DK2921050T3 (da) Fremgangsmåde til udbringning af plantebeskyttelsesmidler