PT2844739T - Métodos e composições para gerar progenitores pancreáticos e células beta funcionais a partir de hpscs - Google Patents

Métodos e composições para gerar progenitores pancreáticos e células beta funcionais a partir de hpscs

Info

Publication number
PT2844739T
PT2844739T PT137846481T PT13784648T PT2844739T PT 2844739 T PT2844739 T PT 2844739T PT 137846481 T PT137846481 T PT 137846481T PT 13784648 T PT13784648 T PT 13784648T PT 2844739 T PT2844739 T PT 2844739T
Authority
PT
Portugal
Prior art keywords
hpscs
compositions
methods
beta cells
pancreatic progenitors
Prior art date
Application number
PT137846481T
Other languages
English (en)
Inventor
Keller Gordon
Cristina Nostro Maria
Holtzinger Audrey
Sarangi Farida
Original Assignee
Univ Health Network
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Health Network filed Critical Univ Health Network
Publication of PT2844739T publication Critical patent/PT2844739T/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/39—Pancreas; Islets of Langerhans
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00—Drugs for disorders of the endocrine system
    • A61P5/48—Drugs for disorders of the endocrine system of the pancreatic hormones
    • A61P5/50—Drugs for disorders of the endocrine system of the pancreatic hormones for increasing or potentiating the activity of insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
PT137846481T 2012-04-30 2013-04-30 Métodos e composições para gerar progenitores pancreáticos e células beta funcionais a partir de hpscs PT2844739T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261640366P 2012-04-30 2012-04-30

Publications (1)

Publication Number Publication Date
PT2844739T true PT2844739T (pt) 2019-10-01

Family

ID=49514141

Family Applications (1)

Application Number Title Priority Date Filing Date
PT137846481T PT2844739T (pt) 2012-04-30 2013-04-30 Métodos e composições para gerar progenitores pancreáticos e células beta funcionais a partir de hpscs

Country Status (18)

Country Link
US (1) US20150104430A1 (pt)
EP (2) EP3594323A1 (pt)
JP (2) JP6397397B2 (pt)
CN (2) CN104411815B (pt)
AU (1) AU2013255031C1 (pt)
CA (1) CA2871795C (pt)
CY (1) CY1122058T1 (pt)
DK (1) DK2844739T3 (pt)
ES (1) ES2747967T3 (pt)
HK (2) HK1208047A1 (pt)
HR (1) HRP20191677T1 (pt)
HU (1) HUE046271T2 (pt)
IN (1) IN2014DN10053A (pt)
LT (1) LT2844739T (pt)
PL (1) PL2844739T3 (pt)
PT (1) PT2844739T (pt)
SI (1) SI2844739T1 (pt)
WO (1) WO2013163739A1 (pt)

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* Cited by examiner, † Cited by third party
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AU2014248167B2 (en) 2013-04-03 2019-10-10 FUJIFILM Cellular Dynamics, Inc. Methods and compositions for culturing endoderm progenitor cells in suspension
JP6730608B2 (ja) * 2014-05-21 2020-07-29 国立大学法人京都大学 膵芽細胞の製造方法および膵芽細胞を含む膵疾患治療剤
DK3286300T3 (da) 2015-04-24 2021-01-18 Univ Copenhagen Isolering af bona fide pankreatiske stamceller
WO2016170067A1 (en) * 2015-04-24 2016-10-27 University Of Copenhagen Method for production of insulin-producing cells
US11332716B2 (en) 2015-07-27 2022-05-17 The Regents Of The University Of California Methods and compositions for producing pancreatic beta cells
CN106554936B (zh) * 2015-09-30 2020-05-05 海门雨霖细胞科技有限责任公司 诱导人干细胞向肝细胞定向分化的新方法
EP3420073B1 (en) * 2016-02-24 2023-11-08 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
MA45329A (fr) 2016-04-08 2019-02-13 Univ California Production de cellules bêta matures pleinement fonctionnelles à partir de progénitrices pancréatiques humaines
JP7065517B2 (ja) * 2016-07-26 2022-05-12 国立大学法人京都大学 Ｐｔｆ１ａ陽性細胞の製造方法
WO2018159805A1 (ja) * 2017-03-03 2018-09-07 国立大学法人京都大学 膵前駆細胞の製造方法
CN111246864A (zh) * 2017-07-21 2020-06-05 森玛治疗公司 干细胞衍生的胰腺β细胞的重聚
CA2983845C (en) 2017-10-26 2024-01-30 University Of Copenhagen Generation of glucose-responsive beta cells
WO2019099725A1 (en) 2017-11-15 2019-05-23 Semma Therapeutics, Inc. Islet cell manufacturing compositions and methods of use
WO2019142883A1 (ja) 2018-01-18 2019-07-25 第一三共株式会社 ジヒドロインドリジノン誘導体
EP3801577A4 (en) 2018-05-31 2021-07-14 University Health Network (UHN) METHOD AND COMPOSITIONS USING TANKYRASE INHIBITOR FOR GENERATING INSULIN-PRODUCING CELLS
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
AU2018439389A1 (en) 2018-08-30 2021-02-18 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
CN111875674B (zh) * 2018-09-03 2021-12-10 内蒙古创润生物科技有限公司 表皮干细胞向胰腺细胞分化的改进方法
CA3116184A1 (en) * 2018-10-15 2020-04-23 Cynity Co., Ltd. Method for producing stem/precursor cells, by using low molecular weight compound, from cells derived from endodermal tissue or organ
JP7385244B2 (ja) * 2019-06-27 2023-11-22 国立大学法人 東京大学 膵前駆細胞の分離方法
WO2021051256A1 (en) * 2019-09-17 2021-03-25 Center For Excellence In Molecular Cell Science, Chinese Academy Of Sciences Pancreatic pro-endocrine progenitor cells and use thereof
CN114634904B (zh) * 2022-05-17 2022-09-13 天津外泌体科技有限公司 高纯度胰腺祖细胞的产生方法

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Also Published As

Publication number Publication date
SI2844739T1 (sl) 2019-11-29
IN2014DN10053A (pt) 2015-08-14
ES2747967T3 (es) 2020-03-12
EP3594323A1 (en) 2020-01-15
CA2871795A1 (en) 2013-11-07
CA2871795C (en) 2023-03-14
AU2013255031B2 (en) 2018-09-06
CY1122058T1 (el) 2020-11-25
HRP20191677T1 (hr) 2019-12-13
JP2018198620A (ja) 2018-12-20
EP2844739B1 (en) 2019-06-19
CN104411815B (zh) 2017-11-28
LT2844739T (lt) 2019-10-10
AU2013255031A1 (en) 2014-12-04
JP6397397B2 (ja) 2018-09-26
JP2015519048A (ja) 2015-07-09
CN108004199A (zh) 2018-05-08
HK1208047A1 (en) 2016-02-19
CN108004199B (zh) 2022-03-18
AU2013255031C1 (en) 2018-12-06
HUE046271T2 (hu) 2020-02-28
DK2844739T3 (da) 2019-09-30
HK1254968A1 (zh) 2019-08-02
EP2844739A1 (en) 2015-03-11
EP2844739A4 (en) 2015-12-02
WO2013163739A1 (en) 2013-11-07
PL2844739T3 (pl) 2019-12-31
JP6759293B2 (ja) 2020-09-23
CN104411815A (zh) 2015-03-11
US20150104430A1 (en) 2015-04-16

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