DK3133157T3 - Fremgangsmåde til oprensning af vitamin k-afhængige proteiner - Google Patents

Fremgangsmåde til oprensning af vitamin k-afhængige proteiner Download PDF

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Publication number
DK3133157T3
DK3133157T3 DK16190737.3T DK16190737T DK3133157T3 DK 3133157 T3 DK3133157 T3 DK 3133157T3 DK 16190737 T DK16190737 T DK 16190737T DK 3133157 T3 DK3133157 T3 DK 3133157T3
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DK
Denmark
Prior art keywords
vitamin
purification
procedure
dependent proteins
proteins
Prior art date
Application number
DK16190737.3T
Other languages
English (en)
Inventor
Gustav Gilljam
Stefan Winge
Original Assignee
Octapharma Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Octapharma Ag filed Critical Octapharma Ag
Application granted granted Critical
Publication of DK3133157T3 publication Critical patent/DK3133157T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DK16190737.3T 2010-03-30 2011-03-30 Fremgangsmåde til oprensning af vitamin k-afhængige proteiner DK3133157T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10158511 2010-03-30
US28289510P 2010-04-16 2010-04-16
EP11713216.7A EP2553095B1 (en) 2010-03-30 2011-03-30 A process for purifying vitamin K dependent proteins such as coagulation factor IX

Publications (1)

Publication Number Publication Date
DK3133157T3 true DK3133157T3 (da) 2019-08-12

Family

ID=42154362

Family Applications (2)

Application Number Title Priority Date Filing Date
DK11713216.7T DK2553095T3 (da) 2010-03-30 2011-03-30 Fremgangsmåde til rensning af vitamin k-afhængige proteiner såsom koagulationsfaktor ix
DK16190737.3T DK3133157T3 (da) 2010-03-30 2011-03-30 Fremgangsmåde til oprensning af vitamin k-afhængige proteiner

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK11713216.7T DK2553095T3 (da) 2010-03-30 2011-03-30 Fremgangsmåde til rensning af vitamin k-afhængige proteiner såsom koagulationsfaktor ix

Country Status (16)

Country Link
US (1) US20130079498A1 (da)
EP (2) EP3133157B1 (da)
JP (1) JP5876868B2 (da)
KR (1) KR101829860B1 (da)
CN (1) CN102858971B (da)
AU (2) AU2011234521B2 (da)
BR (1) BR112012024550B1 (da)
CA (1) CA2793136C (da)
DK (2) DK2553095T3 (da)
ES (2) ES2740825T3 (da)
IL (2) IL221609A (da)
MX (1) MX2012011066A (da)
RU (2) RU2590726C2 (da)
TR (1) TR201911082T4 (da)
WO (1) WO2011121020A1 (da)
ZA (1) ZA201207227B (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105777862A (zh) 2012-10-24 2016-07-20 建新公司 使用mes和mops作为流动相改性剂从多峰树脂洗脱生物分子
CN105175486A (zh) * 2015-10-20 2015-12-23 上海洲跃生物科技有限公司 一种高纯人凝血因子ix的制备方法
CN105330736A (zh) * 2015-11-06 2016-02-17 上海洲跃生物科技有限公司 一种从去冷胶血浆中同时制备人凝血因子ix及vii的方法
CN105326859A (zh) * 2015-11-09 2016-02-17 上海洲跃生物科技有限公司 一种从Cohn血浆组分III中制备人凝血酶原复合物的方法
US11174284B2 (en) * 2017-04-04 2021-11-16 Scarab Genomics, Llc Purification of CRM 197 from bacteria
CN107460182B (zh) * 2017-09-01 2020-12-18 上海太阳生物技术有限公司 一种制备活化猪血浆凝血因子x的方法
CN109444422A (zh) * 2018-12-17 2019-03-08 福建省立医院 一种基于upt-lf定量测定血清pivka-ii的检测卡及其构建方法及应用
CN111378029B (zh) * 2018-12-29 2023-05-05 四川远大蜀阳药业有限责任公司 一种人凝血因子ix的制备方法
CN110257358B (zh) * 2019-06-10 2023-05-19 广东双林生物制药有限公司 一种高纯人凝血因子ix制剂的生产方法
CN111925450B (zh) * 2020-09-21 2020-12-15 迈威(上海)生物科技股份有限公司 一种去除毕赤酵母表达重组蛋白聚集体和/或降解片段的方法
CN113267390B (zh) * 2021-05-11 2023-05-05 北京丹大生物技术有限公司 一种用于滤纸干血片的检测标志物洗脱液及其应用
AU2022357575A1 (en) * 2021-09-28 2024-04-11 Kashiv Biosciences, Llc An improved process for purification of protein

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
US4540573A (en) 1983-07-14 1985-09-10 New York Blood Center, Inc. Undenatured virus-free biologically active protein derivatives
DK162233C (da) * 1989-11-09 1992-03-16 Novo Nordisk As Fremgangsmaade til isolering af faktor viii fra blodplasma og pharmaceutisk praeparat indeholdende den saaledes isolerede fator viii
EP0573605B1 (en) * 1991-03-01 2000-08-30 Rhone-Poulenc Rorer International (Holdings) Inc. Preparation of factor ix
US5286849A (en) * 1992-07-14 1994-02-15 Alpha Therapeutic Corporation Purification of factor IX
DE19506633A1 (de) * 1995-02-25 1996-08-29 Octapharma Ag Verfahren zur Herstellung von Faktor IX aus biologischen Quellen
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
DE69901569T2 (de) * 1998-08-28 2002-12-19 Genentech Inc Humane antikörper gegen faktor ix/ixa
RU2321633C2 (ru) * 2001-02-16 2008-04-10 Ново Нордиск Хелт Кэр Аг Способ получения витамин к-зависимых белков
SE0203551D0 (sv) * 2002-12-02 2002-12-02 Biovitrum Ab Prothrombin purification
US20040106779A1 (en) * 2002-12-03 2004-06-03 Bigler Douglas E. Modified factor IX preparation
EP1718386A1 (en) 2004-02-27 2006-11-08 GE Healthcare Bio-Sciences AB A process for the purification of antibodies
CN102924565A (zh) 2004-06-07 2013-02-13 厄普弗朗特色谱公司 血浆或者血清蛋白的分离
JP2008511546A (ja) * 2004-06-07 2008-04-17 エーブイティー プラズマ リミテッド タンパク質単離のためのプロセス
JP2008525381A (ja) * 2004-12-23 2008-07-17 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少
EP1707634A1 (en) 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins
EP1739179A1 (en) 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
RU2493163C2 (ru) 2007-07-11 2013-09-20 Ново Нордиск А/С Способ очистки белка фактора свертывания viii и способ стабилизации белка фактора viii
EP2027875A1 (en) 2007-08-23 2009-02-25 Octapharma AG A Process for Isolation and Purification of a Target Protein free of Prion Protein (PrPSC)
KR101804136B1 (ko) * 2008-06-24 2017-12-04 옥타파마 아게 응고 인자 ｖｉｉｉ을 정제하는 방법

Also Published As

Publication number Publication date
US20130079498A1 (en) 2013-03-28
ES2740825T3 (es) 2020-02-06
RU2016122883A3 (da) 2019-12-03
IL221609A (en) 2017-01-31
JP2013523689A (ja) 2013-06-17
AU2015203388B2 (en) 2017-11-16
IL248549B (en) 2020-06-30
AU2011234521A1 (en) 2012-09-13
DK2553095T3 (da) 2017-01-23
IL248549A0 (en) 2016-12-29
CA2793136A1 (en) 2011-10-06
RU2016122883A (ru) 2018-11-29
ES2610529T3 (es) 2017-04-28
AU2011234521B2 (en) 2015-04-23
BR112012024550A2 (pt) 2015-09-22
CA2793136C (en) 2019-05-07
EP2553095A1 (en) 2013-02-06
RU2590726C2 (ru) 2016-07-10
AU2015203388A1 (en) 2015-07-09
KR20130010474A (ko) 2013-01-28
BR112012024550B1 (pt) 2020-12-08
EP3133157B1 (en) 2019-05-08
CN102858971B (zh) 2016-06-01
JP5876868B2 (ja) 2016-03-02
TR201911082T4 (tr) 2019-08-21
ZA201207227B (en) 2013-06-26
CN102858971A (zh) 2013-01-02
RU2012146084A (ru) 2014-05-10
EP2553095B1 (en) 2016-10-12
EP3133157A1 (en) 2017-02-22
KR101829860B1 (ko) 2018-02-19
WO2011121020A1 (en) 2011-10-06
MX2012011066A (es) 2012-10-10
RU2731720C2 (ru) 2020-09-08

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