DK2440655T3 - Fremgangsmåde til behandling af pyropheophytin-holdige sammensætninger - Google Patents

Fremgangsmåde til behandling af pyropheophytin-holdige sammensætninger Download PDF

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Publication number
DK2440655T3
DK2440655T3 DK10725286.8T DK10725286T DK2440655T3 DK 2440655 T3 DK2440655 T3 DK 2440655T3 DK 10725286 T DK10725286 T DK 10725286T DK 2440655 T3 DK2440655 T3 DK 2440655T3
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Denmark
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DK10725286.8T
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English (en)
Inventor
Charlotte Horsmans Poulsen
Janne Brunstedt
Rene Mikkelsen
Susan Mampusti Madrid
Masoud Rajabi Zargahi
Jens Frisbæk Sørensen
Jørn Borch Søe
Tina Jørgensen
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Dupont Nutrition Biosci Aps
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Publication of DK2440655T3 publication Critical patent/DK2440655T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40—Colouring or decolouring of foods
    • A23L5/49—Removing colour by chemical reaction, e.g. bleaching
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead

Claims (12)

  1. FREMGANGSMÅDE TIL BEHANDLING AF PYROPHEOPHYTIN-HOLDIGE SAMMENSÆTNINGER PATENTRKAV
    1. Fremgangsmåde til behandling af en pyropheophytin-holdig planteolie, hvilken fremgangsmåde omfatter etablering af kontakt mellem olien og et enzym, der er i stand til at hydrolysere pyropheophytin, hvor enzymet har et forhold mellem pheophytinase- og pyropheophytinaseaktivitet, der er mindre end 80, hvor enzymet omfatter en polypeptidsekvens med mindst 75 % sekvensidentitet med en hvilken som helst ifølge SEQ ID NO: 21, 23 eller 25 over sekvensens fulde længde.
  2. 2. Fremgangsmåde ifølge krav 1 hvor enzymet omfatter en pheophytinase eller pheophytinpheophorbidhydrolase.
  3. 3. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor enzymet er afledt af Arabidopsis thaliana, Populus trichocarpa, Vitis vinifera, Oryza sativa, Zea mays, Nicotiana tabacum, Ostreococcus lucimarinus, Ostreococcus taurii, Physcomitrella patens, Phaeodactylum tricornutum, Chlamydomonas reinhardtii, Triticum aestivum eller Micromonas sp. RCC299.
  4. 4. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor pyropheophytin i olien er hydrolyseret for at danne pyropheophorbid og fremgangsmåden endvidere omfatter et trin med fjernelse af pyropheophorbid fra olien.
  5. 5. Fremgangsmåde ifølge krav 4, hvor fremgangsmåden omfatter et deodoriseringstrin.
  6. 6. Fremgangsmåde ifølge krav 4 eller krav 5, hvor fremgangsmåden omfatter et trin med silicabehandling.
  7. 7. Fremgangsmåde ifølge krav 6, hvor fremgangsmåden omfatter to eller flere silicabehandlingstrin.
  8. 8. Fremgangsmåde ifølge krav 6 eller krav 7, hvor silicabehandlingen udføres ved ca. 70 til 110 °C.
  9. 9. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor olien er udvalgt fra risklid-, soja-, raps-, palme-, oliven-, bomuldsfrø-, majs-, palmekerne-, kokos-, jordnødde-, sesam- eller solsikkeolie.
  10. 10. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor koncentrationen af pyropheophytin i olien reduceres med mindst 10 % sammenlignet med koncentrationen af pyropheophytin til stede i olien før behandling.
  11. 11. Fremgangsmåde ifølge et hvilket som helst foregående krav, der endvidere omfatter et hexanekstraktions- og/eller degummeringstrin.
  12. 12. Anvendelse af et polypeptid med pyropheophytinaseaktivitet til fjernelse af pyropheophytinkontaminering fra en planteolie, hvor enzymet har et forhold mellem pheophytinase- og pyropheophytinaseaktivitet, der er mindre end 80, hvor enzymet omfatter en polypeptidsekvens med mindst 75 % sekvensidentitet med en hvilken som helst ifølge SEQ ID NO: 21, 23 eller 25 over sekvensens fulde længde.
DK10725286.8T 2009-06-12 2010-06-10 Fremgangsmåde til behandling af pyropheophytin-holdige sammensætninger DK2440655T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18652509P 2009-06-12 2009-06-12
US31319310P 2010-03-12 2010-03-12
PCT/IB2010/052581 WO2010143149A2 (en) 2009-06-12 2010-06-10 Method

Publications (1)

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DK2440655T3 true DK2440655T3 (da) 2018-01-15

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DK17189991.7T DK3284820T3 (da) 2009-06-12 2010-06-10 Fremgangsmåde til behandling af pyropheophytin-holdige sammensætninger
DK10725286.8T DK2440655T3 (da) 2009-06-12 2010-06-10 Fremgangsmåde til behandling af pyropheophytin-holdige sammensætninger

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DK17189991.7T DK3284820T3 (da) 2009-06-12 2010-06-10 Fremgangsmåde til behandling af pyropheophytin-holdige sammensætninger

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US (1) US9493748B2 (da)
EP (2) EP2440655B1 (da)
CN (1) CN102803479A (da)
AR (1) AR077042A1 (da)
BR (1) BRPI1010705A2 (da)
CA (1) CA2764023C (da)
DK (2) DK3284820T3 (da)
WO (1) WO2010143149A2 (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011110967A1 (en) * 2010-03-12 2011-09-15 Danisco A/S Process
AR081950A1 (es) * 2010-06-17 2012-10-31 Danisco Proceso de tratamiento de semillas que contienen aceite
CA2825628A1 (en) * 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Method
ES2649912T3 (es) * 2011-02-23 2018-01-16 Dupont Nutrition Biosciences Aps Tratamiento enzimático de la clorofila en los aceites vegetales
WO2013104659A2 (en) * 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) * 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013160372A1 (en) * 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
CN102845620A (zh) * 2012-08-06 2013-01-02 王茜 一种新型安全环保饲料添加剂
EP2792735A1 (de) * 2013-04-16 2014-10-22 Clariant Produkte (Deutschland) GmbH Verfahren zur Verbesserung der wässrigen enzymatischen Entschleimung von Pflanzenölen
CN104630233A (zh) * 2013-11-14 2015-05-20 华中农业大学 OsChls2基因在调控水稻叶绿素含量中的应用
CN105670770A (zh) * 2016-01-28 2016-06-15 广西大学 一种处理含油种子叶绿素的方法
CN106191082A (zh) * 2016-08-08 2016-12-07 山西农业大学 小麦脱镁叶绿素脱镁叶绿酸水解酶基因TaPPH及编码蛋白和克隆方法
CN106590924A (zh) * 2016-12-30 2017-04-26 晨光生物科技集团股份有限公司 一种葡萄籽油的脱色工艺
CN111712568A (zh) 2017-09-26 2020-09-25 邦吉全球创新有限责任公司 从基于三酰基甘油的油酶促除去叶绿素底物
WO2020198212A1 (en) 2019-03-27 2020-10-01 Bunge Global Innovation, Llc Silica adsorbent treatment for removal of chlorophyll derivatives from triacylglycerol-based oils

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5288619A (en) 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
DE4112440C1 (da) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
DE4115938A1 (de) 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
US5315021A (en) 1992-07-01 1994-05-24 The Procter & Gamble Company Process for removing chlorophyll color impurities from vegetable oils
DE4339556C1 (de) 1993-11-19 1995-02-02 Metallgesellschaft Ag Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
EP0835304A1 (en) 1995-06-27 1998-04-15 Unilever N.V. Immobilized enzyme and its use for the processing of triglyceride oils
DE19527274A1 (de) 1995-07-26 1997-01-30 Metallgesellschaft Ag Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase
SE504664C2 (sv) 1995-09-22 1997-03-24 Scotia Lipidteknik Ab Sätt att framställa fraktionerad olja, oljan, dess användning samt emulsionskomposition innehållande oljan
JP4053595B2 (ja) 1995-09-22 2008-02-27 メディカル・リサーチ・カウンシル 核酸の突然変異誘発におけるまたはそれに関する改良
US6344328B1 (en) 1995-12-07 2002-02-05 Diversa Corporation Method for screening for enzyme activity
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
WO1998018912A1 (en) 1996-10-31 1998-05-07 Novo Nordisk A/S Novel phospholipase, production and use thereof
US6103505A (en) 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
MXPA00011569A (es) 1999-03-26 2004-12-03 Diversa Corp Re-ensamble de acidos nucleicos mediado por exonucleasa en evolucion dirigida.
US6376689B1 (en) 1999-09-02 2002-04-23 Cargill, Incorporated Removal of gum and chlorophyll-type compounds from vegetable oils
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
EP1307548A2 (en) 2000-07-13 2003-05-07 Maxygen, Inc. Novel lipase genes
AU2001296577A1 (en) * 2000-10-05 2002-04-15 E.I. Du Pont De Nemours And Company Chlorophyllases
JP4894102B2 (ja) 2001-07-23 2012-03-14 タカタ株式会社 布シートの車体への取付構造
DE60334718D1 (de) 2002-05-20 2010-12-09 Danisco Us Inc Peptidderivate und deren verwendung zur synthese v
EP2468853B1 (en) 2004-06-16 2015-04-01 DSM IP Assets B.V. Method for enzymatic decolorization of pheophytin
WO2006031699A2 (en) 2004-09-10 2006-03-23 Diversa Corporation Compositions and methods for making and modifying oils

Also Published As

Publication number Publication date
EP2440655B1 (en) 2017-12-06
CA2764023C (en) 2018-10-16
WO2010143149A2 (en) 2010-12-16
DK3284820T3 (da) 2020-12-07
CA2764023A1 (en) 2010-12-16
AR077042A1 (es) 2011-07-27
US20120149927A1 (en) 2012-06-14
BRPI1010705A2 (pt) 2015-09-15
US9493748B2 (en) 2016-11-15
EP3284820A1 (en) 2018-02-21
EP2440655A2 (en) 2012-04-18
WO2010143149A3 (en) 2011-03-17
CN102803479A (zh) 2012-11-28
EP3284820B1 (en) 2020-09-16

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