DK2678438T3 - Enzymbehandling af chlorophyll i planteolier - Google Patents

Enzymbehandling af chlorophyll i planteolier Download PDF

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Publication number
DK2678438T3
DK2678438T3 DK12706934.2T DK12706934T DK2678438T3 DK 2678438 T3 DK2678438 T3 DK 2678438T3 DK 12706934 T DK12706934 T DK 12706934T DK 2678438 T3 DK2678438 T3 DK 2678438T3
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Denmark
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gly
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DK12706934.2T
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English (en)
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Jørn Borch Søe
Tina Lillan Jørgensen
Lene Lauridsen
Janne Brunstedt
Rene Mikkelsen
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Dupont Nutrition Biosci Aps
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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
    • A23D9/04—Working-up
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01014—Chlorophyllase (3.1.1.14)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Claims (13)

  1. ENZYMBEHANDLING AF CHLOROPHYLLI PLANTEOLIER
    1. Fremgangsmåde til behandling af en planteolie, hvilken fremgangsmåde omfatter et trin med etablering af kontakt mellem olien og et enzym, hvor enzymet er i stand til at hydrolysere en a’ eller b’ stereoisomer af chlorophyll eller et chlorophyllderivat, og hvor enzymet omfatter et polypeptid med mindst 90 % sekvensidentitet med SEQ ID NO: 13.
  2. 2. Fremgangsmåde ifølge krav 1, hvor a’ eller b’ stereoisomeren omfatter chlorophyll a \ pheophytin a \ chlorophyll b’ eller pheophytin b’.
  3. 3. Fremgangsmåde ifølge krav i eller krav 2, hvor enzymet er i stand til at hydrolysere en a’ stereoisomer af chlorophyll eller chlorophyllderivatet.
  4. 4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor enzymet har et aktivitetsforhold for (a) en a stereoisomer af chlorophyll eller et chlorophyllderivat sammenlignet med en a' stereoisomer af chlorophyll eller chlorophyllderivatet; eller (b) en b stereoisomer af chlorophyll eller et chlorophyllderivat sammenlignet med en b' stereoisomer af chlorophyll eller chlorophyllderivatet; på mindre end 10.
  5. 5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor olien efter behandling med enzymet omfatter (a) mindst 50 % a stereoisomerer af chlorophyll eller chlorophyllderivatet, baseret på den totale mængde af a og a’ stereoisomerer af chlorophyll eller chlorophyllderivatet i olien; eller (b) mindst 50 % b stereoisomerer af chlorophyll eller chlorophyllderivatet, baseret på den totale mængde af b og b' stereoisomerer af chlorophyll eller chlorophyllderivatet i olien.
  6. 6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor enzymet har et aktivitetsforhold for pheophytin sammenlignet med pyropheophytin på mindre end 10.
  7. 7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor enzymet omfatter en chlorophyllase-, pheophytinase- og/eller pyropheophytinaseaktivitet.
  8. 8. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor enzymet omfatter aminosekvensen GHSRG (SEQ ID NO: 32).
  9. 9. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor enzymet er afledt af Ricinus communis.
  10. 10. Anvendelse af et enzym, der er i stand til at hydrolysere chlorophyll eller et chlorophyllderivat, for fjernelse af en a’ eller b’ stereoisomer af chlorophyll eller chlorophyllderivatet fra en planteolie, hvor enzymet har en hydrolytisk aktivitet på mindst 1000 E/g, baseret på aktivitetsenhederne pr. gram af det oprensede enzym, på en prime-stereoisomer af chlorophyll eller et chlorophyllderivat, og hvor en enzymaktivitetsenhed defineres som mængden af enzym, der hydrolyserer en mikromol substrat pr. minut ved 40 °C, hvor assayet til måling af hydrolytisk aktivitet er følgende: a) 170 μΐ 50 mM HEPES, pH 17 tilsættes til 20 μΐ 0,3 mM chlorophyll, pheophytin eller pyropheophytin, der er opløst i acetone; b) enzymet opløses i 50 mM HEPES, pH 7; c) 10 μ 1 enz.ymopløsning tilsættes til 190 μΐ substratopløsning for at initiere reaktionen og inkuberes ved 40 °C i forskellige tidsrum; d) reaktionen stoppes ved tilsætning af 350 μΐ acetone; e) efter centrifugering i 2 min ved 18.000 g analyseres den ovenstående væske ved hjælp af HPLC og mængderne af (i) chlorophyll og chlorophyllid (ii) pheophytin og pheophorbid eller (iii) pyropheophytin og pyropheophorbid bestemmes; hvor enzymet omfatter et polypeptid med mindst 90 % sekvensidentitet med SEQ ID NO: 2 eller SEQ ID NO: 13.
  11. 11. Anvendelse ifølge krav 10, hvor enzymet har et aktivitetsforhold for (a) en a stereoisomer af chlorophyll eller et chlorophyllderivat sammenlignet med en a’ stereoisomer af chlorophyll eller chlorophyllderivatet; eller (b) en b stereoisomer af chlorophyll eller et chlorophyllderivat sammenlignet med en b’ stereoisomer af chlorophyll eller chlorophyllderivatet; på mindre end 10.
  12. 12. Anvendelse ifølge krav 10 eller krav 11, hvor enzymet har et aktivitetsforhold for pheophytin sammenlignet med pyropheophytin på mindre end 10.
  13. 13. Anvendelse ifølge et hvilket som helst af kravene 10 til 12, hvor a’ eller b’ stereoisomeren omfatter chlorophyll a\ pheophytin a\ chlorophyll b’ eller pheophytin b’.
DK12706934.2T 2011-02-23 2012-02-16 Enzymbehandling af chlorophyll i planteolier DK2678438T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161445665P 2011-02-23 2011-02-23
PCT/IB2012/050715 WO2012114234A1 (en) 2011-02-23 2012-02-16 Process

Publications (1)

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DK2678438T3 true DK2678438T3 (da) 2017-10-02

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Country Status (10)

Country Link
US (1) US9493749B2 (da)
EP (1) EP2678438B1 (da)
CN (2) CN107156333A (da)
AR (2) AR085250A1 (da)
BR (1) BR112013021432B1 (da)
CA (2) CA2825632C (da)
DK (1) DK2678438T3 (da)
EA (1) EA201391213A1 (da)
ES (1) ES2649912T3 (da)
WO (1) WO2012114234A1 (da)

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5288619A (en) 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
DE4112440C1 (da) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
DE4115938A1 (de) 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
JP2937746B2 (ja) 1993-04-25 1999-08-23 昭和産業株式会社 油脂の精製方法
DE4339556C1 (de) 1993-11-19 1995-02-02 Metallgesellschaft Ag Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
BR9609381A (pt) 1995-06-27 1999-05-18 Unilever Nv Processo para a imobilização de uma enzima partícula sólida processos catalisados por uma enzima anfifílica para a interesterficação de mono- di- ou triglicerídeos catalisado por uma fosfo-lipase e para a remoção de fosfo-lipídeos de óleo de triglicerídeo
DE19527274A1 (de) 1995-07-26 1997-01-30 Metallgesellschaft Ag Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase
SE504664C2 (sv) 1995-09-22 1997-03-24 Scotia Lipidteknik Ab Sätt att framställa fraktionerad olja, oljan, dess användning samt emulsionskomposition innehållande oljan
AU6996396A (en) 1995-09-22 1997-04-09 Medical Research Council Improvements in or relating to mutagenesis of nucleic acids
US6344328B1 (en) 1995-12-07 2002-02-05 Diversa Corporation Method for screening for enzyme activity
WO2000058517A1 (en) 1999-03-26 2000-10-05 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
DE69721805T2 (de) 1996-09-18 2004-03-11 Merck & Co., Inc. Sordarin und dessen derivate als fungizide für planzenkulturen
US6103505A (en) 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
DK1131416T3 (da) 1998-11-27 2009-10-26 Novozymes As Lipolytiske enzymvarianter
US6376689B1 (en) 1999-09-02 2002-04-23 Cargill, Incorporated Removal of gum and chlorophyll-type compounds from vegetable oils
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
EP1307548A2 (en) 2000-07-13 2003-05-07 Maxygen, Inc. Novel lipase genes
WO2002029022A2 (en) 2000-10-05 2002-04-11 E. I. Du Pont De Nemours And Company Chlorophyllases
CN102174546A (zh) 2002-04-19 2011-09-07 维莱尼姆公司 磷脂酶，编码磷脂酶的核酸以及制备和应用磷脂酶的方法
EP2290085B1 (en) 2003-01-17 2017-12-13 DuPont Nutrition Biosciences ApS Method for the in situ production of an emulsifier in a foodstuff
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
CA2570872A1 (en) 2004-06-16 2006-01-26 Diversa Corporation Compositions and methods for enzymatic decolorization of chlorophyll
US20080070291A1 (en) * 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
MX2007000626A (es) 2004-07-16 2007-03-07 Danisco Enzima lipolitica; usos de la misma en la industria alimenticia.
WO2006031699A2 (en) 2004-09-10 2006-03-23 Diversa Corporation Compositions and methods for making and modifying oils
WO2007055735A2 (en) 2005-05-31 2007-05-18 Verenium Corporation Hydrolases, nucleic acids encoding them and methods for making and using them
EP1987142A4 (en) 2006-02-02 2009-07-15 Verenium Corp ESTERASES AND ASSOCIATED NUCLEIC ACIDS AND METHODS
WO2008143679A2 (en) 2006-06-01 2008-11-27 Verenium Corporation Nucleic acids and proteins and methods for making and using them
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
EP2582809A1 (en) * 2010-06-17 2013-04-24 DuPont Nutrition Biosciences ApS Process

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Publication number Publication date
CN107156333A (zh) 2017-09-15
US9493749B2 (en) 2016-11-15
CA2825632C (en) 2020-07-07
CN103459606B (zh) 2017-06-16
EP2678438A1 (en) 2014-01-01
BR112013021432B1 (pt) 2021-04-20
AR085250A1 (es) 2013-09-18
CA3004088A1 (en) 2012-08-30
US20130330804A1 (en) 2013-12-12
EP2678438B1 (en) 2017-07-05
ES2649912T3 (es) 2018-01-16
US20140363877A9 (en) 2014-12-11
CN103459606A (zh) 2013-12-18
WO2012114234A1 (en) 2012-08-30
CA2825632A1 (en) 2012-08-30
EA201391213A1 (ru) 2014-05-30
AR116290A2 (es) 2021-04-21
BR112013021432A2 (pt) 2016-10-25

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