DE68925893D1 - Sekretorische leader-sequenzen - Google Patents

Sekretorische leader-sequenzen

Info

Publication number
DE68925893D1
DE68925893D1 DE68925893T DE68925893T DE68925893D1 DE 68925893 D1 DE68925893 D1 DE 68925893D1 DE 68925893 T DE68925893 T DE 68925893T DE 68925893 T DE68925893 T DE 68925893T DE 68925893 D1 DE68925893 D1 DE 68925893D1
Authority
DE
Germany
Prior art keywords
leu
ser
phe
ile
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68925893T
Other languages
English (en)
Other versions
DE68925893T2 (de
Inventor
Andrew Goodey
Graham Belfield
Darrell Sleep
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Albumedix Ltd
Original Assignee
Delta Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888817598A external-priority patent/GB8817598D0/en
Priority claimed from GB898906920A external-priority patent/GB8906920D0/en
Application filed by Delta Biotechnology Ltd filed Critical Delta Biotechnology Ltd
Publication of DE68925893D1 publication Critical patent/DE68925893D1/de
Application granted granted Critical
Publication of DE68925893T2 publication Critical patent/DE68925893T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
DE68925893T 1988-07-23 1989-07-14 Sekretorische leader-sequenzen Expired - Lifetime DE68925893T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB888817598A GB8817598D0 (en) 1988-07-23 1988-07-23 Dna & peptide sequences
GB898906920A GB8906920D0 (en) 1989-03-28 1989-03-28 Peptide and dna sequences
PCT/GB1989/000816 WO1990001063A1 (en) 1988-07-23 1989-07-14 New secretory leader sequences

Publications (2)

Publication Number Publication Date
DE68925893D1 true DE68925893D1 (de) 1996-04-11
DE68925893T2 DE68925893T2 (de) 1996-08-08

Family

ID=26294196

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68925893T Expired - Lifetime DE68925893T2 (de) 1988-07-23 1989-07-14 Sekretorische leader-sequenzen

Country Status (15)

Country Link
US (1) US5302697A (de)
EP (1) EP0387319B1 (de)
JP (1) JP3092811B2 (de)
KR (1) KR100195632B1 (de)
AT (1) ATE135045T1 (de)
AU (1) AU633020B2 (de)
CA (1) CA1340547C (de)
DE (1) DE68925893T2 (de)
DK (1) DK175853B1 (de)
FI (1) FI104564B (de)
GB (1) GB2230268B (de)
HU (1) HU213571B (de)
IE (1) IE62266B1 (de)
IL (1) IL91024A (de)
WO (1) WO1990001063A1 (de)

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CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5759802A (en) * 1988-10-26 1998-06-02 Tonen Corporation Production of human serum alubumin A
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
US5766883A (en) * 1989-04-29 1998-06-16 Delta Biotechnology Limited Polypeptides
FR2650598B1 (fr) * 1989-08-03 1994-06-03 Rhone Poulenc Sante Derives de l'albumine a fonction therapeutique
DK300090D0 (da) * 1990-12-19 1990-12-19 Novo Nordisk As Fremgangsmaade til fremstilling af leadersekvenser
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US5521086A (en) * 1993-09-16 1996-05-28 Cephalon, Inc. Secretion sequence for the production of a heterologous protein in yeast
EP0773296B1 (de) * 1995-02-03 2006-09-13 Asahi Glass Company Ltd. Gen für ein sekretionssignal und dieses enthaltender vektor
US5712114A (en) * 1995-06-06 1998-01-27 Basf Aktiengesellschaft Compositions for expression of proteins in host cells using a preprocollagen signal sequence
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
GB9712370D0 (en) 1997-06-14 1997-08-13 Aepact Ltd Therapeutic systems
AU1519699A (en) * 1997-11-07 1999-05-31 Conjuchem, Inc. Affinity markers for human serum albumin
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
JP2003530847A (ja) 2000-04-12 2003-10-21 ヒューマン ゲノム サイエンシズ インコーポレイテッド アルブミン融合タンパク質
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
US20050244931A1 (en) * 2001-04-12 2005-11-03 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20060084794A1 (en) * 2001-04-12 2006-04-20 Human Genome Sciences, Inc. Albumin fusion proteins
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
AU2002332041A1 (en) * 2001-10-05 2003-04-22 Human Genome Sciences, Inc. Albumin fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
JP5424521B2 (ja) 2001-12-21 2014-02-26 ヒューマン ジノーム サイエンシーズ， インコーポレイテッド アルブミン融合タンパク質
US20080194481A1 (en) 2001-12-21 2008-08-14 Human Genome Sciences, Inc. Albumin Fusion Proteins
WO2003059934A2 (en) * 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
CA2485064A1 (en) 2002-02-07 2003-08-14 Delta Biotechnology Limited Albumin-fused kunitz domain peptides
GB0217033D0 (en) 2002-07-23 2002-08-28 Delta Biotechnology Ltd Gene and polypeptide sequences
CA2548140A1 (en) 2003-12-05 2005-06-23 University Of Massachusetts Oligosaccharide compositions and use thereof in the treatment of infection
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
EP1702069A2 (de) 2004-01-05 2006-09-20 EMD Lexigen Research Center Corp. Auf onkofötales fibronectin gerichtetes interleukin-12
EP1816201A1 (de) 2006-02-06 2007-08-08 CSL Behring GmbH Modifizierter Koagulationsfaktor VIIa mit verbesserter 'half-life'-Stabiltät
ATE524493T1 (de) 2006-07-24 2011-09-15 Biorexis Pharmaceutical Corp Exendin-fusionsproteine
US9505823B2 (en) 2006-08-07 2016-11-29 TEV A Biopharmaceuticals USA, Inc. Albumin-insulin fusion proteins
WO2008051383A2 (en) * 2006-10-19 2008-05-02 Amgen Inc. Use of alcohol co-solvents to improve pegylation reaction yields
US8043829B2 (en) * 2006-10-25 2011-10-25 Amgen Inc. DNA encoding chimeric toxin peptide fusion proteins and vectors and mammalian cells for recombinant expression
EP2738257A1 (de) 2007-05-22 2014-06-04 Amgen Inc. Zusammensetzungen und Verfahren zur Herstellung bioaktiver Fusionsproteine
EP2604623A3 (de) 2007-08-08 2013-10-02 Novozymes Biopharma DK A/S Transferrinvarianten und Konjugate
US20110294988A1 (en) 2009-02-06 2011-12-01 Novozymes Bio Pharma DK A/S Purification Process
JP5936112B2 (ja) 2009-02-11 2016-06-15 アルブミディクス アクティーゼルスカブ アルブミン変異体及び複合体
JP2012521360A (ja) 2009-03-20 2012-09-13 アムジエン・インコーポレーテツド Ｋｖ１．３の選択的かつ強力なペプチド阻害剤
JP2013511279A (ja) 2009-11-20 2013-04-04 アムジエン・インコーポレーテツド 抗Ｏｒａｉ１抗原結合蛋白及びその使用
CN103314296A (zh) 2010-08-10 2013-09-18 安姆根有限公司 用于检测针对靶抗体的中和抗体的双功能体外靶结合测定
KR20130110169A (ko) 2010-09-22 2013-10-08 암젠 인크 담체 면역글로뷸린 및 이것의 용도
WO2012099983A1 (en) 2011-01-18 2012-07-26 Amgen Inc. Nav1.7 knockout mice and uses thereof
EP2681245B1 (de) 2011-03-03 2018-05-09 Zymeworks Inc. Entwurf und konstrukte für ein multivalentes heteromultimeres gerüst
SG10201601789TA (en) 2011-03-16 2016-04-28 Amgen Inc Potent And Selective Inhibitors Of Nav1.3 And Nav1.7
AU2013222188A1 (en) 2012-02-22 2014-09-11 Amgen Inc. Autologous mammalian models derived from induced pluripotent stem cells and related methods
AU2013289881B2 (en) 2012-07-13 2018-01-18 Zymeworks Bc Inc. Multivalent heteromultimer scaffold design and constructs
US10344060B2 (en) 2013-03-12 2019-07-09 Amgen Inc. Potent and selective inhibitors of Nav1.7
CA2910945A1 (en) 2013-05-08 2014-11-13 Zymeworks Inc. Bispecific her2 and her3 antigen binding constructs
WO2015031686A1 (en) 2013-08-30 2015-03-05 Amgen Inc. High titer recombinant aav vector production in adherent and suspension cells
US10463716B2 (en) 2014-10-13 2019-11-05 University Of Maryland, Baltimore Methods for treating cardiovascular dysfunction and improving fluid homeostasis with Elabela peptide hormone
US10202616B2 (en) 2014-10-15 2019-02-12 Amgen Inc. Promoter and regulatory elements for improved expression of heterologous genes in host cells
CA2874083C (en) 2014-12-05 2024-01-02 Universite Laval Tdp-43-binding polypeptides useful for the treatment of neurodegenerative diseases
BR112018012851A2 (pt) 2015-12-22 2018-12-26 Albumedix Ltd cepas de expressão de proteína melhoradas
EP3408397B1 (de) 2016-01-27 2020-07-15 Just-Evotec Biologics, Inc. Hybrider promoter und verwendungen davon
CN109937034B (zh) 2016-11-21 2022-09-16 济世-伊沃泰克生物制品有限公司 阿柏西普制剂及其用途
EP4026908A1 (de) 2017-06-20 2022-07-13 Albumedix Ltd Verbesserte proteinexpressionsstämme
CA3067735A1 (en) 2017-08-17 2019-02-21 Just Biotherapeutics, Inc. Method of purifying glycosylated protein from host cell galectins and other contaminants
JP7183268B2 (ja) 2017-11-20 2022-12-05 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド 等張化剤としてリジン塩を含有するアフリベルセプト製剤及びその使用
US20220119757A1 (en) 2019-02-15 2022-04-21 Just-Evotec Biologics, Inc. Automated biomanufacturing systems, facilities, and processes
US11739166B2 (en) 2020-07-02 2023-08-29 Davol Inc. Reactive polysaccharide-based hemostatic agent
WO2023287707A1 (en) 2021-07-15 2023-01-19 Just-Evotec Biologics, Inc. Bidirectional tangential flow filtration (tff) perfusion system
WO2023154305A2 (en) 2022-02-10 2023-08-17 Amgen Inc. Antibody protein product expression constructs for high throughput sequencing

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NZ207925A (en) * 1983-04-25 1988-05-30 Genentech Inc Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4588684A (en) * 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
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Also Published As

Publication number Publication date
HU894572D0 (en) 1991-05-28
KR100195632B1 (ko) 1999-06-15
ATE135045T1 (de) 1996-03-15
GB2230268A (en) 1990-10-17
HU213571B (en) 1997-08-28
AU633020B2 (en) 1993-01-21
AU4042789A (en) 1990-02-19
GB8928849D0 (en) 1990-07-18
DK175853B1 (da) 2005-04-04
JPH03500370A (ja) 1991-01-31
WO1990001063A1 (en) 1990-02-08
CA1340547C (en) 1999-05-18
JP3092811B2 (ja) 2000-09-25
IE892361L (en) 1990-01-23
US5302697A (en) 1994-04-12
FI104564B (fi) 2000-02-29
FI901428A0 (fi) 1990-03-21
DE68925893T2 (de) 1996-08-08
HUT57829A (en) 1991-12-30
KR920700288A (ko) 1992-02-19
IE62266B1 (en) 1995-01-11
EP0387319A1 (de) 1990-09-19
DK72990D0 (da) 1990-03-21
IL91024A0 (en) 1990-02-09
IL91024A (en) 1995-08-31
GB2230268B (en) 1992-01-29
DK72990A (da) 1990-03-21
EP0387319B1 (de) 1996-03-06

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Owner name: NOVOZYMES DELTA LTD., NOTTINGHAM, GB