CY1121864T1 - Τρωκτικα που εκφραζουν ευαισθητες στο ρη αλληλουχιες ανοσοσφαιρινης - Google Patents

Τρωκτικα που εκφραζουν ευαισθητες στο ρη αλληλουχιες ανοσοσφαιρινης

Info

Publication number
CY1121864T1
CY1121864T1 CY20191100821T CY191100821T CY1121864T1 CY 1121864 T1 CY1121864 T1 CY 1121864T1 CY 20191100821 T CY20191100821 T CY 20191100821T CY 191100821 T CY191100821 T CY 191100821T CY 1121864 T1 CY1121864 T1 CY 1121864T1
Authority
CY
Cyprus
Prior art keywords
histidine
immunoglobulin
codon
germline
human animals
Prior art date
Application number
CY20191100821T
Other languages
English (en)
Inventor
John Mcwhirter
Lynn Macdonald
Joel H. Martin
Andrew J. Murphy
Original Assignee
Regeneron Pharmaceuticals, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharmaceuticals, Inc. filed Critical Regeneron Pharmaceuticals, Inc.
Publication of CY1121864T1 publication Critical patent/CY1121864T1/el

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Παρέχονται γενετικά τροποποιημένα ζώα πλην του ανθρώπου τα οποία εκφράζουν έναν μεταβλητό τομέα της ανοσοσφαιρίνης που περιλαμβάνει τουλάχιστον μία ιστιδίνη, όπου η τουλάχιστον μία ιστιδίνη κωδικοποιείται από μια υποκατάσταση ενός κωδικονίου πλην της ιστιδίνης στη βλαστική σειρά του ζώου με ένα κωδικόνιο ιστιδίνης ή από την εισαγωγή ενός κωδικονίου ιστιδίνης σε μια νουκλεοξική αλληλουχία ανοσοσφαιρίνης της βλαστικής σειράς. Παρέχονται επίσης γονίδια ανοσοσφαιρίνης που περιλαμβάνουν ιστιδίνες σε μία ή περισσότερες CDR, σε μια Ν-τελική περιοχή ή/και σε μια περιοχή βρόχου 4. Μεταβλητοί τομείς ανοσοσφαιρίνης που περιλαμβάνουν μία ή περισσότερες ιστιδίνες (π.χ. συστάδες ιστιδινών) που υποκαθιστούν μη αντιγονοδεσμευτικά κατάλοιπα πλην της ιστιδίνης. Παρέχονται επίσης ζώα πλην του ανθρώπου τα οποία είναι απόγονοι ζώων που περιλαμβάνουν τροποποιημένους μεταβλητούς γενετικούς τόπους βαριάς αλυσίδας (τμήματα V, D, J), τροποποιημένους μεταβλητούς γενετικούς τόπους ελαφριάς αλυσίδας (τμήματα V, J) και αναδιαταγμένα γονίδια ελαφριάς αλυσίδας της βλαστικής σειράς (αλληλουχίες VJ). Παρέχονται ζώα πλην του ανθρώπου τα οποία φτιάχνουν τομείς ανοσοσφαιρίνης που δεσμεύουν αντιγόνα κατά τρόπο ευαίσθητο στο pΗ.
CY20191100821T 2012-03-16 2019-08-01 Τρωκτικα που εκφραζουν ευαισθητες στο ρη αλληλουχιες ανοσοσφαιρινης CY1121864T1 (el)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261612126P 2012-03-16 2012-03-16
US201261611950P 2012-03-16 2012-03-16
US201261613352P 2012-03-20 2012-03-20
US201261736930P 2012-12-13 2012-12-13
PCT/US2013/032036 WO2013138712A1 (en) 2012-03-16 2013-03-15 Non-human animals expressing ph-sensitive immunoglobulin sequences

Publications (1)

Publication Number Publication Date
CY1121864T1 true CY1121864T1 (el) 2020-07-31

Family

ID=48045752

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20191100821T CY1121864T1 (el) 2012-03-16 2019-08-01 Τρωκτικα που εκφραζουν ευαισθητες στο ρη αλληλουχιες ανοσοσφαιρινης

Country Status (24)

Country Link
US (4) US9801362B2 (el)
EP (2) EP3539374A1 (el)
JP (4) JP6228589B2 (el)
KR (3) KR102213535B1 (el)
CN (2) CN104302171B (el)
BR (1) BR112014022855A2 (el)
CA (1) CA2865645A1 (el)
CY (1) CY1121864T1 (el)
DK (1) DK2825037T3 (el)
ES (1) ES2737990T3 (el)
HK (1) HK1200270A1 (el)
HR (1) HRP20191333T1 (el)
HU (1) HUE045537T2 (el)
IL (4) IL234237B (el)
LT (1) LT2825037T (el)
MX (2) MX355944B (el)
NZ (3) NZ629077A (el)
PL (1) PL2825037T4 (el)
PT (1) PT2825037T (el)
RS (1) RS59076B1 (el)
RU (2) RU2018128915A (el)
SG (3) SG11201405171WA (el)
SI (1) SI2825037T1 (el)
WO (1) WO2013138712A1 (el)

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