CN217479473U

CN217479473U - Biochip all-in-one with picture acquisition mechanism

Info

Publication number: CN217479473U
Application number: CN202221685861.4U
Authority: CN
Inventors: 孙悦; 陈立波
Original assignee: Fist Shanghai Biotechnology Co ltd
Current assignee: Fist Shanghai Biotechnology Co ltd
Priority date: 2022-05-24
Filing date: 2022-07-01
Publication date: 2022-09-23
Anticipated expiration: 2032-07-01
Also published as: CN116410843A; CN217614815U; CN217628334U; CN217479466U; CN217479439U

Abstract

The utility model provides a biochip all-in-one with picture acquisition mechanism, the biochip all-in-one includes hybridization portion and recognition portion, and hybridization portion is equipped with control by temperature change heating member, reactor and shifts subassembly and a plurality of reactor, and the reactor is equipped with inside appearance chamber, inside holds and is equipped with biochip in the chamber, and the front panel and the rear panel of reactor are transparent, inside appearance chamber can see through front panel and rear panel, reactor carry out hybridization reaction in the control by temperature change heating member, and a plurality of reactors are arranged in proper order, and recognition portion is equipped with picture acquisition mechanism, and the reactor shifts the subassembly and connects and drives a plurality of reactors and get into the recognition district from the hybridization reaction district and supply picture acquisition mechanism to shoot or return to the temperature control heating member from the recognition district with a plurality of reactors. The utility model discloses but camera straight line reciprocating motion to carry out disposable complete shooting to a plurality of reactors on the reactor frame, do benefit to the integration setting of biochip hybridization and result recognition.

Description

Biochip all-in-one with picture acquisition mechanism

Technical Field

The utility model belongs to the technical field of the biochip, specifically be a biochip all-in-one with picture acquisition mechanism.

Background

A biochip hybridization instrument is an analytical instrument which is generally used in the biological field, and a nucleic acid molecular hybridization technology is adopted to detect whether a genome to be detected contains a known gene sequence. When the hybridization instrument is used, liquid (hybridization liquid, binding liquid, sample liquid and the like) needs to be put into the hybridization instrument for reaction, and the reaction result can be judged by the color, the shape and the like after the reaction. After the reaction of the liquid in the hybridization instrument is completed, the state of the reacted reaction body generally needs to be shot for subsequent analysis and storage, and if the reaction body is shot manually by people, the efficiency is obviously low.

SUMMERY OF THE UTILITY MODEL

The above-mentioned problem to prior art exists, the utility model aims at providing a biochip all-in-one with picture acquisition mechanism, through picture acquisition mechanism shoots the reactor, in order to acquire the state of reactor after the reaction, but camera straight line reciprocating motion, in order to carry out disposable complete shooting to a plurality of reactors on the reactor frame, it is efficient, data are accurate to shoot, the reactor frame corresponds the department and sets up to the fretwork, in order not to block the shooting of camera, the drive assembly and the reactor that the cooperation set up shift the subassembly and can promote the reactor frame, in order to aim at the camera with it and shoot, after finishing shooting, reset the reactor frame, in order to overcome the blockking of heating block to the chip, do benefit to the integration setting of biochip hybridization and result recognition.

In order to realize the purpose, the utility model discloses the technical scheme who adopts is:

the utility model provides a biochip all-in-one with picture acquisition mechanism, the biochip all-in-one includes hybridization portion and recognition portion, and hybridization portion is equipped with the control by temperature change heating member, the reactor shifts subassembly and a plurality of reactor, and the reactor is equipped with inside appearance chamber, inside is equipped with biochip in holding the chamber, and the front panel and the rear panel of reactor are transparent, inside appearance chamber can see through front panel and rear panel, the reactor carries out hybridization reaction in the control by temperature change heating member, and a plurality of reactors are arranged in proper order, and recognition portion is equipped with picture acquisition mechanism, and the reactor shifts the subassembly and connects and drives a plurality of reactors and get into the recognition district from the hybridization reaction district and supply picture acquisition mechanism to shoot or return the temperature control heating member with a plurality of reactors from the recognition district.

As a further improvement of the above technical solution:

hybridization portion still is equipped with the support body, and the reactor is installed on the support body, is equipped with front panel and rear panel that fretwork department does not stop the reactor on the support body, and support body and reactor shift the subassembly and connect, and the reactor shifts the subassembly and drives the support body and remove, and the support body drives a plurality of reactors and removes to get into the recognition district at most a plurality of reactors and supply the picture to acquire the mechanism and shoot or to get into the control by temperature change heating member from the recognition district at most a plurality of reactors.

The image acquisition mechanism comprises a camera component, a third sliding rail and a camera driving piece, the third sliding rail is fixedly installed in the reading part, the three length directions of the sliding rails are parallel to the arrangement direction of the plurality of reactors, the camera component is arranged on the third sliding rail in a sliding mode, and the camera driving piece is connected with and drives the camera component to reciprocate along the third sliding rail.

The camera driving part comprises a motor III and a plurality of belt wheels II, a transmission belt is sleeved on the belt wheels II, the camera component is connected on the transmission belt, and the motor III is connected with and drives one of the belt wheels II to rotate.

The image acquisition mechanism further comprises an electric wire assembly, the electric wire assembly comprises a sliding rod and a second spring wire, the sliding rod is fixedly installed on the recognition portion, the spring wire is sleeved outside the sliding rod, the electric wire connected with the camera assembly is located in the second spring wire, one end of the second spring wire is connected with the camera assembly, and the other end of the second spring wire is connected with an external control system.

The control by temperature change heating member includes thermobloc and heating film, and the thermoblock is equipped with inside and holds the chamber, and inside thermoblock upper end opening, the upper end that the support body can follow the thermoblock got into or left the thermoblock, and the heating film cladding is outside the thermoblock, and the electric wire will be connected heating film and external power source, and the heating film generates heat after the circular telegram, and the heat passes through the thermoblock and transmits to the reactor.

The hybridization part is also provided with a driving component which is connected with and drives the reactor transfer component to ascend or descend.

Hybridization portion still is equipped with the sealing strip, and the sealing strip is connected with the support body, and reactor transfer assembly includes magnet mount, electro-magnet, connecting rod and return spring, and the magnet mount is installed on drive assembly, and the electro-magnet is installed on the magnet mount, and return spring's one end is connected the magnet mount is connected to connecting rod, the other end, can produce the effort to the connecting rod of metal material after the electro-magnet circular telegram, and the drive connecting rod removes, makes the connecting rod insert in the connecting hole of seting up on the sealing strip, and return spring is stretched simultaneously, after the electro-magnet outage, the connecting rod returns under return spring's spring action, breaks away from the contact with the sealing strip.

The driving assembly comprises a second motor, a second linear guide rail and a driving plate, the second motor is driven to the driving plate through the second linear guide rail in a transmission mode to drive the driving plate to ascend or descend, and the magnet fixing frame is installed on the driving plate.

The utility model has the advantages that: through picture acquisition mechanism shoots the reactor, in order to acquire the state of reaction back reactor, but camera straight line reciprocating motion, in order to carry out once only complete shooting to a plurality of reactors on the reactor frame, it is efficient, data are accurate to shoot, reactor frame correspondence department sets up to the fretwork, in order not to block the shooting of camera, the drive assembly and the reactor that the cooperation set up shift the subassembly and can promote reactor frame, in order to aim at the camera with it and shoot, shoot and reset reactor frame after finishing, in order to overcome the blockking of heating block to the chip, do benefit to the integration setting of biochip hybridization and result recognition.

Drawings

FIG. 1 is a schematic exterior view of an all-in-one machine of the present invention.

FIG. 2 is a schematic view of the structure of one embodiment of the present invention with the center cover panel, front panel and pull handle removed.

Fig. 3 is a schematic structural view of fig. 2 with the shooting assistance assembly removed.

Fig. 4 is a schematic view of the structure of fig. 3 with the thermal block removed.

FIG. 5 is a diagram illustrating a structure of a picture taking assembly according to an embodiment of the present invention.

FIG. 6 is a schematic diagram of a reactor and syringe configuration according to one embodiment of the present invention.

Figure 7 is a schematic view of the drive plate, drive rod and reactor transfer assembly configuration of one embodiment of the present invention.

FIG. 8 is a schematic view of a seal bead configuration according to an embodiment of the present invention.

Detailed Description

The following describes in detail embodiments of the present invention with reference to the accompanying drawings. It should be understood that the description herein is provided for illustration and explanation of the invention and is not intended to limit the invention.

Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

A biochip integrated machine with an image acquisition mechanism is disclosed, as shown in figures 1-8, and comprises a shell, and a hybridization part and a reading part in the shell. The reaction vessel 402 provided with the biochip is hybridized in the hybridization reaction region of the hybridization section, and the reading section is used to read the result of the biochip in the reaction vessel 402 after hybridization.

The shell comprises a lower mounting plate 101, a middle cover plate 102, a rear panel 103 and a front panel 104, wherein the lower mounting plate 101, the middle cover plate 102, the rear panel 103 and the front panel 104 are connected to form a shell of the biochip all-in-one machine. Wherein, the lower mounting plate 101 is arranged at the bottom of the biochip all-in-one machine, the back plate 103 and the front plate 104 are oppositely arranged at two ends of the lower mounting plate 101, and the middle cover plate 102 covers the top surface and two opposite side surfaces of the biochip all-in-one machine. Inside the housing is provided an upper main board 105 on which other components are mounted. Preferably, the upper main board 105 and the lower mounting board 101 are arranged in parallel and spaced, and the shape of the upper main board 105 can be flexibly designed according to the arrangement of the components. The rear panel 103 is provided with a power switch and a USB interface, and the power switch turns on or off a circuit where the electric equipment in the shell is located. The USB interface can be connected with a control system such as a computer and the like to collect data and input control instructions.

The hybridization part is provided with a liquid containing component 2, a liquid transferring component 3, a reactor frame 5, a temperature control heating component, a sealing pressing strip 7, a reactor taking and placing component, a piston component, a reactor transferring component 10, a driving component 11 and a plurality of reactors 402,

the liquid containing assembly 2 is used for containing liquid, and the liquid containing assembly 2 comprises a test tube groove tray 201 and a test tube groove 202. The upper surface parallel interval of test tube groove tray 201 has the recess of a plurality of rectangular shapes, the recess is used for placing the test tube that holds liquid. The test tube well tray 201 is detachably supported on the test tube well 202.

The liquid transferring component 3 is connected with and drives the liquid containing component 2 to move. Further, the liquid containing assembly 2 is driven to reciprocate to move linearly, and when the liquid containing assembly 2 moves linearly, the liquid containing assembly extends out of or retracts into the shell. Specifically, the pipetting assembly 3 comprises a first motor and a first linear guide rail. The test tube groove 202 is installed on the linear guide rail I, the motor I transmits and drives the test tube groove 202 through the linear guide rail I, and the test tube groove 202 drives the test tube groove tray 201 to move linearly.

Reactor 402 is provided with an interior volume in which the biochip is disposed. The front and rear panels of the reactor 402 through which the interior volume can be viewed or photographed are transparent. The reactor 402 is connected with a needle tube 401, preferably, the reactor 402 is fixedly connected with the needle tube 401, one end of the needle tube 401 is connected to the reactor 402, the other end of the needle tube 401 is suspended, and the inner channel of the needle tube 401 is communicated with the inner cavity of the reactor 402. The upper end of the reactor 402 is provided with a through hole which is communicated with the inner cavity of the reactor 402.

The reactor frame 5 is used for installing and supporting the reactor 402, and a plurality of installation positions for installing the reactor 402 are arranged on the reactor frame 5 and arranged in a row in parallel at intervals. The upper end of the reactor frame 5 is also provided with a plurality of through holes which are respectively communicated with the plurality of installation positions. The reactor 402 is removably mounted on the mounting station. The reactor frame 5 is detachably mounted on the upper main plate 105, and the reactor frame 5 is positioned above the liquid containing assembly 2. After the reactor 402 is installed on the reactor frame 5, the reactor 402 is clamped into the installation position, the inner cavity of the reactor 402, the through hole at the upper end of the reactor 402 and the through hole at the upper end of the reactor frame 5 are sequentially communicated, and in order to improve the sealing property of the communicated channel, a sealing ring can be arranged at the joint of the upper end of the reactor 402 and the reactor frame 5. In particular, the reactor 402 is snapped into the installation position from the side of the reactor frame 5. The transparent front panel and the transparent rear panel of the reactor 402 are not shielded by the reactor frame 5, and the corresponding reactor frame 5 is hollowed out to allow the subsequent image acquisition mechanism to take images. The open end of the needle tube 401 is downward, the needle tube 401 extends out of the bottom of the reactor frame 5, and the lower ends of the needle tubes 401 of the plurality of reactors 402 on the plurality of mounting positions are respectively engaged with the plurality of grooves on the test tube well tray 201. By the subsequent action of the piston assembly and the drive assembly 11, the fluid in the cuvette tray 201 can be drawn into the reactor 402 through the needle 401.

The temperature-controlled heating element includes a heat block 601, a heating film, and an electric wire, and the heat block 601 is used to heat the reactor 402 so that the reaction in the reactor 402 provides a temperature condition. The hot block 601 is provided with an internal cavity, the upper end of the hot block 601 is open, and the lower end of the hot block 601 is provided with a plurality of holes at intervals, the holes are communicated with the internal cavity, the reactor frame 5 can be placed into the hot block 601 from the upper end of the hot block 601, and meanwhile, the needle tubes 401 on the reactors 402 on the reactor frame 5 respectively penetrate through the holes at the lower end of the hot block 601 and then extend out of the hot block 601. I.e. the reactor frame 5 is placed directly in the thermoblock 601 without connecting the two by means of a connecting member. Both ends of the thermal block 601 are supported on the upper main plate 105. That is, the reactor frame 5 is not directly supported on the upper main plate 105 but is supported on the upper main plate 105 by the thermal block 601. The outside of the hot block 601 is coated with a heating film, the heating film is connected with an external power supply through a wire to heat the heating film, and heat is transferred to the reactor 402 through the hot block 601. When the reactor frame 5 is placed on the thermal block 601, the top of the reactor frame 5 is higher than the top of the thermal block 601.

The sealing pressing strip 7 is detachably connected with the upper end of the reactor frame 5, and the upper end face of the reactor frame 5 is attached to the lower end face of the sealing pressing strip 7 after connection. The sealing pressing strip 7 comprises a pressing strip body 701, and a first transverse block 702 and a second transverse block 703 which are positioned on the same surface of the pressing strip body 701. The first transverse block 702 and the second transverse block 703 are arranged in parallel at intervals, and the first transverse block 702 is positioned below the second transverse block 703. A plurality of parallel through holes are arranged in a row at intervals on the first transverse block 702, a plurality of parallel through holes are arranged in a row at intervals on the second transverse block 703, and the plurality of through holes on the first transverse block 702 and the plurality of through holes on the second transverse block 703 are on the same straight line. The number of the through holes on the first transverse block 702, the number of the through holes on the second transverse block 703 and the number of the installation positions on the reactor frame 5 are equal. Preferably, the aperture of the through hole on the first cross block 702 is equal to the aperture of the through hole on the second cross block 703. When the sealing pressing bar 7 is connected with the reactor frame 5, the plurality of through holes on the first cross block 702 are respectively communicated with the plurality of through holes at the upper end of the reactor frame 5. In order to improve the sealing performance of the communicated channel, a sealing ring can be arranged at the joint of the sealing pressing strip 7 and the reactor frame 5. The pressing strip body 701 is further provided with a blind hole-shaped connecting hole, and the connecting hole and the first cross block 702 are respectively located on two side faces of the pressing strip body 701.

The reactor pick-and-place assembly is used to bring or retract the reactor frame 5 out of or into the housing. The reactor pick-and-place assembly includes a handle 801, two sliders one 802, two slide rails one 803, and two spring wires one 804. Two first sliding rails 803 are arranged on two sides of the thermal block 601 at intervals in parallel, and the first sliding rails 803 and the thermal block 601 are perpendicular to each other. The first slide rail 803 is fixedly installed on the upper main board 105, and the first slide block 802 can slide linearly along the first slide rail 803. The two ends of the thermal block 601 are respectively connected and supported on the two first sliding blocks 802. The two ends of the handle 801 are respectively connected and supported on the two sliders one 802 or the two ends of the thermal block 601. When the handle 801 is pulled, the thermal block 601, the reactor frame 5 and the sealing pressing bar 7 are driven to move linearly by the two sliding blocks one 802, so that the thermal block 601, the reactor frame 5 and the sealing pressing bar can synchronously extend out of the shell or retract back to the shell. The handle 801 is provided on the front panel 104.

In order to allow the reactor frame 5 to be accurately returned to the initial position and stably held, two spring wires one 804 are provided. Two spring wires I804 are arranged in parallel at intervals, one end of each spring wire I804 is connected with the upper main board 105, and the other end of each spring wire I804 is connected with one end of one sliding block I802 or one end of the thermal block 601. The first spring wire 804 is in a stretched state, and when the reactor frame 5 and the thermal block 601 are pulled out, the stretched length of the first spring wire 804 is increased, and when the reactor frame 5 and the thermal block 601 are retracted to the set positions, the first spring wire 804 is stopped by the stopper to continue to retract, and at this time, the first spring wire 804 is still in a stretched state, so that the reactor frame 5 and the thermal block 601 can be stably and reliably located at the set positions. Preferably, the electrical wires connected to the heating film outside the thermal block 601 are located inside the spring wire one 804, i.e. the spring wire one 804 serves the dual function of energizing the thermal block 601 and stabilizing the position of the reactor frame 5.

The piston assembly includes a plurality of pistons 901 and a plurality of springs. One end of the piston 901 is provided with a stopper, the outer diameter of which is larger than the outer diameter of the piston 901. The outer diameter of the piston 901 is not larger than the aperture of the through hole on the first cross block 702 of the sealing pressing strip 7, and the outer diameter of the limiting head of the piston 901 is larger than the aperture of the through hole on the first cross block 702 and the aperture of the through hole on the second cross block 703. The piston 901 can extend into the through hole of the first cross block 702, and the circumferential surface of the piston 901 is attached to the wall of the through hole, so that the piston 901 can seal one end of the through hole of the first cross block 702. One end of the piston 901, which is far away from the limiting head, extends into the through hole of the first cross block 702, and the other end of the piston is located between the first cross block 702 and the second cross block 703, that is, the limiting head is located between the first cross block 702 and the second cross block 703, and the limiting head cannot extend into the through hole of the first cross block 702 or the through hole of the second cross block 703. The pistons 901 respectively extend into the through holes in the first cross block 702, and the pistons 901, the through holes in the first cross block 702 and the through holes in the second cross block 703 respectively share a center line. The spring is located between the first cross block 702 and the second cross block 703 and sleeved outside the piston 901, specifically, one end of the spring is connected to the top of the first cross block 702, and the other end of the spring is connected to the limiting head of the piston 901.

The reactor transfer assembly 10 includes a magnet holder, an electromagnet, a connecting rod, and a return spring. The electro-magnet is installed on the magnet mount, and the connecting rod passes through return spring to be installed on the magnet mount, and the connecting rod is arranged with lower mounting panel 101 parallel interval. After the electromagnet is electrified, acting force is generated on the connecting rod made of metal materials, the connecting rod is driven to move, the connecting rod is inserted into a connecting hole in the pressing strip body 701 of the sealing pressing strip 7, the driving assembly 11 drives the magnet fixing frame to lift, the sealing pressing strip 7 and the reactor frame 5 are driven to lift through the connecting rod, and the reactor frame 5 can be separated from the hot block 601 after lifting. When the electromagnet is powered off, the connecting rod returns under the elastic force of the return spring, and the connecting rod is withdrawn from the sealing pressing strip 7 and is separated from the sealing pressing strip 7.

The drive assembly 11 may engage and actuate the piston assembly to draw fluid into or out of the reactor 402. Meanwhile, the driving assembly 11 can drive the sealing pressing strip 7 and the reactor frame 5 to lift.

The driving assembly 11 includes a second motor, a module fixing plate, a second linear guide rail, a driving plate 1104 and a plurality of driving rods 1105. The module fixing plate is fixedly installed on the upper main plate 105, the second linear guide rail is installed on the module fixing plate, the transmission plate 1104 is connected to the second linear guide rail, and the second motor transmits drive to the transmission plate 1104 through the second linear guide rail, so that the transmission plate 1104 ascends or descends.

The linear guide rail II comprises a guide rail seat, a screw rod II and a nut connecting block II, the screw rod II is rotatably installed on the guide rail seat, the screw rod II is perpendicular to the horizontal plane and is in threaded connection with the screw rod II, and when the screw rod II rotates, the screw rod II drives the nut connecting block II to move on the guide rail seat along the length direction of the screw rod II.

And the module fixing plate is also provided with two sliding blocks II and two sliding rails II. The second sliding rails and the screw rod are arranged in parallel, and the two second sliding rails are respectively arranged on two sides of the screw rod. The two second sliding blocks are respectively arranged on the two second sliding rails in a sliding manner. One end of the transmission plate 1104 is simultaneously connected with a second nut connecting block and a second sliding block, the second nut connecting block drives the transmission plate 1104 to move synchronously, and the second sliding block is used for guiding the movement of the transmission plate 1104, so that the transmission plate 1104 can move more stably.

A plurality of drive links 1105 are spaced in parallel in a row, with one end of the drive links 1105 fixedly attached to the drive plate 1104 and the other end cantilevered downwardly. The number of drive rods 1105 is the same as the number of pistons 901. The outer diameter of the transmission rod 1105 is not larger than the aperture of the through hole on the second cross block 703. The transmission rods 1105 are respectively concentric with the through holes on the second cross block 703. When the transmission plate 1104 descends, the transmission rods 1105 are driven to descend and respectively pass through the through holes in the second cross block 703, then respectively contact one ends of the limiting heads of the pistons 901, when the transmission rods 1105 continue to descend, the pistons 901 in contact with the transmission rods can be pressed down, and the springs are compressed. When the transmission rod 1105 ascends, the piston 901 is driven by the spring to ascend due to the action of the spring, and when the transmission rod 1105 enters the through hole on the second cross block 703, the piston 901 is separated from the contact with the transmission rod 1105 because the limit head of the piston 901 cannot enter the through hole on the second cross block 703.

The magnet fixing frame of the reactor transfer assembly 10 is fixedly installed on the bottom surface of the transmission plate 1104, that is, the reactor transfer assembly 10 is driven to ascend or descend by the driving assembly 11.

The recognition part is provided with a picture acquisition mechanism which shoots the reacted reactor 402 and transmits the shot information to a control system electrically connected with the reactor. The picture taking mechanism comprises a camera assembly 1201, a slide rail three 1202, a camera driving member, a shooting auxiliary assembly 1204 and a wire assembly.

The third slide rail 1202 is mounted on the upper main plate 105, and the length direction of the third slide rail 1202 is parallel to the arrangement direction of the plurality of reactors 402 on the reactor rack 5, i.e. parallel to the arrangement direction of the plurality of mounting positions on the reactor rack 5.

The camera assembly 1201 is slidably disposed on the third slide rail 1202. The camera assembly 1201 is used for shooting the reactor 402, and the camera assembly 1201 includes camera support, camera and camera hood, and the camera support slides and establishes on three 1202 slide rails, and the camera is installed on the camera support, and camera hood covers outside the camera, exposes the camera lens of camera.

The camera is connected with an external control system and a power supply through a wire assembly. The wire assembly comprises a sliding rod 12051 and a second spring wire 12052, the sliding rod 12051 is erected on the upper main board 105, the second spring wire 12052 is sleeved outside the sliding rod 12051, the second spring wire 12052 can move along the sliding rod 12051, a wire connected with the camera assembly 1201 is positioned in the second spring wire 12052, one end of the second spring wire 12052 is connected with the camera assembly 1201, and the other end of the second spring wire 12052 is connected with an external control system so as to transmit a shot picture or video to the control system. The second spring wire 12052 is movably disposed to accommodate the change in position of the camera.

The shooting assistance component 1204 includes a backlight, a light barrier, and the like, and the backlight and the light barrier provide qualified light conditions for shooting. Both ends of the backlight and the light barrier are connected to the thermal block 601 or the first sliding rail 803, namely, when the handle 801 is pulled, the backlight and the light barrier move synchronously along with the thermal block 601 and the reactor frame 5.

The camera driving member is connected to and drives the camera assembly 1201 to move along the third slide rail 1202 to photograph the plurality of reactors 402 on the reactor rack 5. The camera driving member comprises a third motor 12031 and a plurality of second belt wheels 12032, wherein a transmission belt is sleeved on the second belt wheels 12032, namely the transmission belt connects the second belt wheels 12032, the camera assembly 1201 is connected to the transmission belt, and specifically, the camera support is connected to the transmission belt. The third motor 12031 is connected with and drives one of the second belt wheels 12032 to rotate, the second belt wheel 12032 drives the transmission belt to move through the matching of the other second belt wheels 12032, and the transmission belt drives the camera assembly 1201 connected with the transmission belt to move. The back and forth movement of the camera assembly 1201 on the rail three 1202 may be controlled by controlling the rotation of the motor three 12031. In this embodiment, four second belt wheels 12032 are provided, the four second belt wheels 12032 are located on four vertexes of a rectangle, one side of the rectangle is parallel to the third sliding rail 1202, and the camera support is connected to the side parallel to the third sliding rail 1202.

All the motors, the picture acquisition mechanism, the circuit switch where the electromagnet is located and the circuit switch where the heating film of the hot block 601 is located are electrically connected with an external control system through USB interfaces.

The utility model discloses a use method and working process do:

the first step is as follows: the reactor 402 is mounted to the reactor frame 5.

Firstly, pulling the handle 801, the handle 801 drives the thermal block 601 or the sliding block I802, so that the thermal block 601 moves along the sliding rail I803 through the sliding block I802 and moves out of the shell, and simultaneously the thermal block 601 drives the reactor frame 5 placed on the thermal block I, the sealing pressing strip 7 connected with the reactor frame 5 and the piston assembly on the sealing pressing strip 7 to synchronously extend out.

Then, the sealing bead 7 and the reactor frame 5 connected thereto are taken out, and the plurality of reactors 402 are respectively inserted from the side surfaces of the reactor frame 5, so that the reactors 402 are stably mounted on the mounting positions of the reactor frame 5, and the needle tubes 401 downwardly overhang the bottom of the reactor frame 5.

Finally, the reactor frame 5 with the reactor 402 installed and the sealing bead 7 are placed in the thermal block 601 with the reactor frame 5 facing downward and the plurality of needle tubes 401 respectively passing through the plurality of holes at the lower end of the thermal block 601 and protruding out of the thermal block 601. The handle 801 is retracted, the first spring wire 804 drives the first sliding block 802, the thermal block 601, the reactor frame 5 and the sealing and pressing strip 7 to retract to the initial position, and the initial position is stably maintained under the tension of the first spring wire 804.

The second step is that: and connecting a USB interface on the back panel 103 of the biochip all-in-one machine with a computer or other control systems, and turning on a power supply.

The third step: the test tube filled with the liquid is put into the test tube well tray 201.

Firstly, a first motor is started through a control system, a first screw rod 3032 of the first motor driving a linear guide rail rotates, the first screw rod 3032 drives a first nut connecting block 3033 to move along the length direction of the first screw rod, and the first nut connecting block 3033 drives a test tube groove 202 and a test tube groove tray 201 connected with the first screw rod to move, so that the test tube groove 202 and the test tube groove tray 201 extend out of a shell.

Then, the test tube filled with the liquid is placed on the groove of the test tube well tray 201. The preferred, the recess complex liquid box of setting and test-tube groove tray 201, the liquid box includes box body and test-tube rack, and the test-tube rack is installed on the box body, be equipped with a plurality of test tubes on the test-tube rack, a plurality of test tubes are used for the different liquid of splendid attire respectively. The test-tube rack is also provided with a through hole communicated with the box body, and the box body is also used for receiving waste liquid. The direction of the array of the plurality of test tubes is parallel to the moving direction of the test tube well tray 201.

The fourth step: starting the first motor, enabling the first motor to drive the test tube groove 202 and the test tube groove tray 201 to operate according to a set program, enabling the reactor 402 to simultaneously suck liquid and discharge liquid, and enabling the thermal block 601 to be electrified and heated.

In this step, the test tube well tray 201 moves stepwise. The fourth step includes the following steps:

step S1: the reactor transfer assembly 10 lifts the reactor rack 5 so that the needle tubes 401 do not interfere with the movement of the test tubes on the test tube well tray 201.

First, the circuit of the electromagnet is turned on, and the connection hole of the sealing bead 7 is aligned with the connection rod of the reactor transfer unit 10 on the driving plate 1104 in the initial position. After the electromagnet is electrified, acting force is generated between the electromagnet and the connecting rod, the connecting rod is driven to be inserted into the connecting hole in the pressing strip body 701 of the sealing pressing strip 7, and the electromagnet is kept electrified.

And then, starting the second motor, transmitting the power of the second motor to the transmission plate 1104 through the second linear guide rail, driving the transmission plate 1104 to ascend by the second linear guide rail, driving the connecting rod on the transmission plate 1104 to ascend, and driving the sealing pressing strip 7, the reactor frame 5 and the reactor 402 to ascend synchronously by the connecting rod.

Step S2: the tube well tray 201 moves, and when a tube filled with a liquid is moved to a position right under the needle tube 401, the tube well tray 201 stops moving.

Step S3: the reactor transfer unit 10 lowers the reactor frame 5 so that the needle tubes 401 are inserted into the test tubes.

In this step, the second motor is started in the reverse direction, the transmission plate 1104 and the connecting rod are driven to descend, and the connecting rod drives the sealing pressing bar 7, the reactor frame 5 and the reactor 402 to descend synchronously, so that the reactor frame 5 enters and is supported on the hot block 601 again.

Step S4: the piston assembly is actuated and the needle 401 draws the fluid in the test tube into the interior volume of the reactor 402.

In this step, first, the second motor is started by the control system, so that the power of the second motor is transmitted to the transmission plate 1104 through the second linear guide rail, at this time, the circuit connected with the electromagnet is in a disconnected state, and the connecting rod of the reactor transfer assembly 10 is not connected with the sealing pressing strip 7. The transmission plate 1104 is driven to descend by the second linear guide rail, the transmission rods 1105 on the transmission plate 1104 respectively extend into and penetrate through the through holes on the second cross block 703, after the transmission rods 1105 respectively contact the pistons 901, the pistons 901 are pressed, the pistons 901 respectively descend along the through holes on the first cross block 702, and at the moment, the springs outside the pistons 901 are compressed. As the piston 901 descends, the through holes below the piston 901 on the first cross block 702 and the gas in the cavity of the reactor 402 are exhausted outward.

Then, the second motor is started in a reverse direction, so that the power of the second motor is transmitted to the transmission plate 1104 through the second linear guide rail, the transmission plate 1104 is driven by the second linear guide rail to rise, the piston 901 rises under the elastic force of the spring until the limit head of the piston 901 contacts the bottom of the second cross block 703, and cannot rise any more, and the spring is still in a compressed state at the moment, so that the current state of the piston 901 can be stably maintained. During the process of piston 901 rising, the liquid in the test tube will be sucked into the cavity of the reactor 402 through the needle tube 401, similar to the principle of a syringe. The drive plate 1104 continues to rise to the initial position.

Step S5: the reactor transfer unit 10 lifts the reactor frame 5, and the test tube well tray 201 moves, and when the test tube well tray 201 moves to a position where the opening of the chamber for containing the waste liquid is located below the needle tube 401, the movement of the test tube well tray 201 stops.

Step S6: the piston assembly is actuated and the liquid in the chamber of the reactor 402 is drained. The operation of the piston assembly in this step is the same as that in step S4.

Step S7: the steps S1 to S6 are repeated to suck the liquid in another test tube into the needle tube 401.

The reaction is complete when the reactors 402 have completed their sequential aspiration of liquid into the tubes. After each aspiration of liquid, a procedure is performed in reactor 402, such as hybridization, washing, coloration, etc.

During the reaction, the thermal block 601 will transfer heat to the reactor 402. Specifically, a circuit where the heating film outside the thermal block 601 is located is conducted, the heating film is electrified and heated, and heat is transferred to the reactor 402 inside the thermal block 601 through the thermal block 601 to provide reaction conditions for the liquid in the reactor 402.

The fifth step: after the reaction is complete, each reactor 402 is photographed and the photographed pictures or videos are saved.

First, the reactor transfer assembly 10 lifts the reactor frame 5 so that the reactor 402 is exposed and at the same height as the camera assembly 1201.

Then, the camera driving part is started to drive the camera assembly 1201 to move along the third slide rail 1202, and the reactors 402 are sequentially photographed.

Finally, the reactor transfer assembly 10 lowers the reactor frame 5.

And a sixth step: the liquid cartridge in the cuvette tray 201 is removed and the reactor 402 is dismantled.

Finally, it must be said here that: the above embodiments are only used for further detailed description of the technical solutions of the present invention, and should not be understood as limiting the scope of the present invention, and the modifications and adjustments made by those skilled in the art according to the above-mentioned contents of the present invention are all included in the scope of the present invention.

Claims

1. The utility model provides a biochip all-in-one with picture acquisition mechanism, its characterized in that, the biochip all-in-one includes hybridization portion and recognition portion, and hybridization portion is equipped with the control by temperature change heating member, reactor shifts subassembly (10) and a plurality of reactor (402), and reactor (402) are equipped with inside appearance chamber, inside holds and is equipped with biochip in the chamber, and the front panel and the rear panel of reactor (402) are transparent, inside appearance chamber can see through front panel and rear panel, and reactor (402) carry out hybridization reaction in the control by temperature change heating member, and a plurality of reactors (402) arrange in proper order, and recognition portion is equipped with picture acquisition mechanism, and reactor shift subassembly (10) are connected and are driven a plurality of reactors (402) and get into the recognition district from the hybridization reaction district and supply picture acquisition mechanism to shoot or with a plurality of reactors (402) from the recognition district to send back to the control by temperature change heating member.

2. The biochip all-in-one machine according to claim 1, wherein: hybridization portion still is equipped with the support body, reactor (402) are installed on the support body, be equipped with front panel and rear panel that fretwork department does not block reactor (402) on the support body, support body and reactor shift subassembly (10) are connected, reactor shift subassembly (10) drive support body and remove, the support body drives a plurality of reactors (402) and removes to a plurality of reactors (402) and get into the district of reading and supply the picture to get the mechanism and shoot or to a plurality of reactors (402) from the district of reading and get into the control by temperature change heating member.

3. The all-in-one biochip manufacturing machine according to claim 1 or 2, wherein: the picture acquisition mechanism comprises a camera assembly (1201), a third sliding rail (1202) and a camera driving piece, wherein the third sliding rail (1202) is fixedly installed on the reading part, the length direction of the third sliding rail (1202) is parallel to the arrangement direction of the plurality of reactors (402), the camera assembly (1201) is arranged on the third sliding rail (1202) in a sliding mode, and the camera driving piece is connected with and drives the camera assembly (1201) to move back and forth along the third sliding rail (1202).

4. The all-in-one biochip maker according to claim 3, wherein: the camera driving piece comprises a motor III (12031) and a plurality of belt wheels II (12032), a transmission belt is sleeved on the belt wheels II (12032), the camera component (1201) is connected to the transmission belt, and the motor III (12031) is connected with one of the belt wheels II (12032) and drives the same to rotate.

5. The all-in-one biochip maker according to claim 3, wherein: the picture acquisition mechanism further comprises a wire assembly, the wire assembly comprises a sliding rod (12051) and a second spring wire (12052), the sliding rod (12051) is fixedly installed on the reading part, the second spring wire (12052) is sleeved outside the sliding rod (12051), a wire connected with the camera assembly (1201) is located in the second spring wire (12052), one end of the second spring wire (12052) is connected with the camera assembly (1201), and the other end of the second spring wire (12052) is connected with an external control system.

6. The biochip all-in-one machine according to claim 2, wherein: the temperature control heating member comprises a hot block (601) and a heating film, wherein an inner containing cavity is formed in the hot block (601), the upper end of the hot block (601) is open, the frame body can enter or leave the hot block (601) from the upper end of the hot block (601), the heating film is coated outside the hot block (601), the heating film is connected with an external power supply through an electric wire, the heating film generates heat after being electrified, and the heat is transferred to the reactor through the hot block (601).

7. The biochip integrated machine of claim 2, wherein: the hybridization part is also provided with a driving component (11), and the driving component (11) is connected with and drives the reactor transfer component (10) to ascend or descend.

8. The biochip all-in-one machine according to claim 7, wherein: the cross portion still is equipped with sealing strip (7), and sealing strip (7) are connected with the support body, and reactor transfer assembly (10) are including magnet mount, electro-magnet, connecting rod and return spring, and the magnet mount is installed on drive assembly (11), and the electro-magnet is installed on the magnet mount, and return spring's one end is connected the magnet mount is connected to connecting rod, the other end, and the electro-magnet circular telegram back can produce the effort to metal material's connecting rod, and the drive connecting rod removes, makes the connecting rod insert in the connecting hole of seting up on sealing strip (7), and return spring is stretched simultaneously, after the electro-magnet outage, the connecting rod returns under return spring's spring action, breaks away from the contact with sealing strip (7).

9. The all-in-one biochip maker according to claim 8, wherein: the driving assembly (11) comprises a second motor, a second linear guide rail and a transmission plate (1104), the second motor is driven to the transmission plate (1104) through the second linear guide rail in a transmission mode to drive the transmission plate (1104) to ascend or descend, and the magnet fixing frame is installed on the transmission plate (1104).