CN115216396A - Nucleic acid detection device - Google Patents

Abstract

The embodiment of the application provides a nucleic acid detection device, and relates to the technical field of detection equipment. The nucleic acid detection device comprises a bottom plate, a shell, a magnetic frame assembly, an extraction heating assembly, a PCR module, a driving module, an injector assembly and a ventilation and filtration system; the magnetic frame assembly is arranged on the bottom plate and comprises a magnetic rod and a first driving mechanism, the first driving mechanism drives the magnetic rod to move along a preset direction, the magnetic rod is arranged on one side of the to-be-tested agent frame, and magnetic beads are arranged in the to-be-tested agent frame; the extraction heating assembly is arranged on the bottom plate; the PCR module is arranged on the bottom plate, and the extraction heating assembly is matched with the PCR module; the driving module comprises an X-axis module, a Y-axis module and a Z-axis module, the X-axis module is arranged on the bottom plate, the Y-axis module is arranged on the X-axis module, the X-axis module drives the Y-axis module to move in the left and right directions, the Z-axis module is arranged on the Y-axis module, and the Y-axis module drives the Z-axis module to move back and forth; the ventilation filter system is mounted inside the housing.

Description

Nucleic acid detection device

Technical Field

The application relates to the technical field of detection equipment, in particular to a nucleic acid detection device.

Background

At present, the existing nucleic acid detecting instrument generally adopts a magnetic bead extraction method, wherein the nucleic acid detecting instrument is a single device, and a fluorescent Polymerase Chain Reaction (PCR) instrument is a device, and the detection is carried out in an independent laboratory. The magnetic bead is extracted and is used the bar magnet, and the bar magnet can reciprocate and remove about with, and the bar magnet outside also has the bar magnet cover, a plastic sheath promptly, and the plastic sheath effect is for making bar magnet and magnetic bead liquid separate, and the bar magnet inserts the bar magnet cover, and the bar magnet cover inserts 96 orifice plates together with the bar magnet in, makes the magnetic bead gathering or makes the magnetic bead transfer to No. 2 No. 3 No. 4 No. 5 isoporous hole from No. 1 hole. Most of the current market uses a fluorescent PCR instrument for interpretation, and simultaneously, a light-emitting Diode (LED) lamp emits light, the light passes through an optical fiber and hits the liquid in the PCR tube, the liquid in the PCR tube hits the returned optical fiber after reflecting the light, and the optical fiber transmits the light to a Photodiode (PD) receiver. The PCR instrument is also heated or cooled by a heat radiator and a semiconductor Cooler (TEC).

In the prior art, a nucleic acid detector needs a special experimental field, extraction equipment needs to occupy a laboratory, a fluorescence PCR instrument is equipment and needs to occupy an independent laboratory, the occupied area is large, and the detection efficiency is low.

Disclosure of Invention

An object of the embodiments of the present application is to provide a nucleic acid detecting apparatus that can achieve a technical effect of improving detection efficiency.

The embodiment of the application provides a nucleic acid detection device, which comprises a bottom plate, a shell, a magnetic frame assembly, an extraction heating assembly, a PCR module, a driving module, an injector assembly and a ventilation and filtration system, wherein the bottom plate is provided with a magnetic frame;

the magnetic rack assembly is arranged on the bottom plate and comprises a magnetic rod and a first driving mechanism, the first driving mechanism drives the magnetic rod to move along a preset direction, the magnetic rod is arranged on one side of the reagent rack to be tested, and magnetic beads are arranged in the reagent rack to be tested;

the extraction heating assembly is mounted on the base plate;

the PCR module is arranged on the bottom plate, and the extraction heating assembly is matched with the PCR module;

the driving module comprises an X-axis module, a Y-axis module and a Z-axis module, the X-axis module is installed on the bottom plate, the Y-axis module is installed on the X-axis module, the X-axis module drives the Y-axis module to move in the left-right direction, the Z-axis module is installed on the Y-axis module, and the Y-axis module drives the Z-axis module to move back and forth;

the ventilation filter system is mounted inside the housing.

In the implementation process, the nucleic acid detection device can independently and fully automatically complete the detection process through one device without manual carrying after consumable materials such as a PCR tube, a reagent bottle, a gun head and the like are placed by assembling the magnetic frame assembly, the extraction heating assembly, the PCR module, the driving module, the injector assembly and the ventilation filtering system together; moreover, by arranging the ventilation and filtration system, biological cross contamination and biological safety hazards are avoided. The nucleic acid detection device has small volume and can realize the technical effect of improving the detection efficiency.

Further, the device also comprises a bottle locking component;

the bottle locking assembly comprises an electric locking gripper and an electric screwing-cover gripper, the electric screwing-cover gripper is used for gripping the reagent bottle to be detected and then moving the reagent bottle to be detected to the position of the electric locking gripper, the reagent bottle to be detected is locked through the electric locking gripper, and the reagent bottle to be detected is unscrewed through the electric screwing-cover gripper.

In the implementation process, the locking and the uncovering actions of the reagent bottle to be tested are realized through the mutual matching of the locking electric gripper and the cover screwing electric gripper, and the subsequent detection flow is conveniently realized.

Further, the PCR module comprises a circuit board, a PCR tube seat, a TEC mechanism and a heat radiator;

the TEC mechanism is mounted to the heat sink, and the surface of the TEC mechanism is coated with heat-conducting silicone grease;

the PCR tube seat is arranged on the upper surface of the TEC mechanism;

the circuit board is provided with an LED lamp and a detection sensor, the LED lamp emits a detection light beam to the PCR tube, the detection sensor receives the detection light beam reflected by the PCR tube, and the detection sensor identifies the liquid information of the PCR tube according to the detection light beam.

Furthermore, the magnetic frame assembly also comprises a triangular fixing plate, a magnetic frame bottom plate, an optical axis seat, an optical axis, a linear bearing, a driving mounting seat and a movable plate;

the triangular fixing plate is mounted to a magnetic frame bottom plate, the optical axis seat is fixed to the magnetic frame bottom plate, and the optical axis is mounted to the optical axis seat;

the linear bearing is fixedly installed to the movable plate, the first driving mechanism is installed to the driving installation seat, and the driving installation seat is installed to the magnetic frame bottom plate.

Furthermore, the magnetic frame component also comprises a first induction sheet, a first photoelectric switch, a photoelectric switch seat and a magnetic rod seat;

the first induction sheet is installed to the movable plate, the first photoelectric switch is installed to the photoelectric switch base, the photoelectric switch base is installed to the magnetic frame bottom plate, the magnetic rod is installed to the magnetic rod base, the magnetic rod base is installed to the movable plate, and the movable plate and the magnetic rod base are driven by the first driving mechanism to enable the magnetic rod to move up and down.

Further, the Z-axis module comprises a guide rail, a Z-axis bottom plate, a screwing cover electric gripper movable plate, a gun head movable plate, a guide rail slide block, a gun head linear motor and a screwing cover electric gripper linear motor;

the guide rail is mounted to the Z-axis base plate;

the gun head linear motor and the screwing cover electric gripper linear motor are respectively mounted to the linear mounting base.

Furthermore, the device also comprises an X-axis induction sheet, a second induction sheet, a gun head link, a gun head rod and a needle release plate;

the X-axis induction sheet is mounted to the linear motor base, and the second induction sheet is mounted to the gun head movable plate and the screwing cover electric gripper movable plate;

the gun head link is mounted to the gun head rod, and the gun head rod is mounted to the gun head movable plate;

the stripper plate is mounted to the Z-axis bed.

Further, the device also comprises a reagent rack assembly, wherein the reagent rack assembly comprises a pressing strip, a first supporting plate, a second supporting plate, a reagent rack supporting plate and a reagent bottle supporting plate;

the pressing strip is mounted to the reagent rack support plate;

the first support plate and the second support plate are respectively mounted to the reagent rack support plate;

the reagent bottle support plate is mounted to the reagent rack support plate.

Further, the extraction heating assembly comprises a first heating plate, a heating assembly base, a heating film, a pressing plate, a temperature protector and a temperature protector pressing plate;

the first heater plate is mounted to the heater assembly base and the heater film is mounted to the first heater plate;

the clamp plate is installed to first hot plate, temperature protector, the temperature protector clamp plate is installed respectively to the clamp plate.

The device further comprises a heat cover assembly, wherein the heat cover assembly comprises a heat insulation plate, a second heating plate, a sliding block, a heat cover guide rail, a second driving mechanism, a second driving installation seat, a third induction sheet and a second photoelectric switch;

the heat insulation plate is mounted to the second heating plate;

the sliding plate is mounted to the second heating plate and the sliding block is mounted to the hot cover rail;

the second drive mechanism is mounted to the second drive mount;

the hot cover rail is fixed to the panel, the sliding block is fixed to the sliding plate, the third sensing piece is fixedly mounted to the sliding plate, and the second photoelectric switch is fixed to the panel.

Additional features and advantages of the disclosure will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by the practice of the above-described techniques.

In order to make the aforementioned objects, features and advantages of the present application comprehensible, preferred embodiments accompanied with figures are described in detail below.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments of the present application will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and that those skilled in the art can also obtain other related drawings based on the drawings without inventive efforts.

FIG. 1 is a schematic diagram showing an exploded structure of a nucleic acid detecting apparatus according to an embodiment of the present application;

FIG. 2 is a schematic diagram of an exploded structure of a PCR module according to an embodiment of the present disclosure;

FIG. 3 is a schematic structural diagram of a PCR module provided in an embodiment of the present application;

FIG. 4 is an exploded view of a magnetic carrier assembly according to an embodiment of the present application;

fig. 5 is an exploded view of a Z-axis module according to an embodiment of the present disclosure;

FIG. 6 is a schematic diagram of an explosive mechanism of a reagent rack assembly provided in an embodiment of the present application;

fig. 7 is an exploded view of an extraction heating assembly according to an embodiment of the present disclosure;

fig. 8 is an exploded view of a thermal cover assembly according to an embodiment of the present disclosure.

Icon: a base plate 10; a housing 11; a middle fixing plate 12; a ground leg 13; a power supply 14; a bracket 15; an ultraviolet lamp 16; a front door 17; a rear cover 18; the back cover HEPA air purifier filters the cotton 19; a rear cover fan 110; a consumable support plate assembly 111; a middle baffle 112; a sealing tape 113; a thermal lid cover 114; a display screen 115; a hot lid assembly 116; a bottom fan 117; a panel 118; a magnetic carrier assembly 20; a magnetic rod 21; a first drive mechanism 22; a triangular fixing plate 23; a magnetic frame bottom plate 24; a light axis seat 25; an optical axis 26; a linear bearing 27; a drive mount 28; a movable plate 29; a first sensing piece 210; a first photoelectric switch 211; a photoelectric switch base 212; a magnet bar base 213; an extraction heating assembly 30; a first heating plate 31; a heating assembly base 32; heating the film 33; a platen 34; a temperature protector 35; a temperature protector platen 36; a PCR module 40; a circuit board 41; a PCR tube holder 42; a TEC mechanism 43; a heat sink 44; an LED lamp 45; a detection sensor 46; a PCR tube 47; an LED indicator 48; a wire cover 49; a drive module 50; an X-axis module 51; a Y-axis module 52; a Z-axis module 53; a guide rail 54; a Z-axis base plate 55; covering the electric gripper moving plate 56; a gun head movable plate 57; a rail slider 58; a gun head linear motor 59; screwing the electric gripper linear motor 510; an X-axis sensor chip 511; a second sensing piece 512; a lance head link 513; a nosepiece bar 514; a needle removing plate 515; a linear mount 516; a third photoelectric switch 517; a lance tip 518; a reagent bottle 519; a guide sleeve cover plate 520; a guide sleeve 521; a stop block 522; a syringe assembly 60; a ventilation filter system 70; the filtration system housing 71; a blower 72; a blower bracket plate 73; operating area HEPA air purifier filter cotton 74; a filtration system baffle 75; a bottle locking assembly 80; locking the electric gripper 81; screw-capping the electric gripper 82; a reagent rack assembly 90; a bead 91; a first support plate 92; a second support plate 93; a reagent rack support plate 94; a reagent bottle support plate 95; a front support plate 96; a reagent rack 97; a lance tip 98; a hot lid assembly 100; a heat insulating board 1001; a second heating plate 1002; a sliding plate 1003; a slider 1004; a hot lid rail 1005; a second drive mechanism 1006; a second drive mount 1007; a third sensing piece 1008; a second photoelectric switch 1009; a second heating film 1010; a linear motor nut 1011; linear motor lead screw 1012.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present application, as presented in the figures, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.

In the present application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal", and the like indicate an orientation or positional relationship based on the orientation or positional relationship shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.

Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as appropriate.

Furthermore, the terms "mounted," "disposed," "provided," "connected," and "connected" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; can be a mechanical connection, or a point connection; either directly or indirectly through intervening media, or may be an internal communication between two devices, elements or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

Furthermore, the terms "first," "second," and the like, are used primarily to distinguish one device, element, or component from another (the specific nature and configuration may be the same or different), and are not used to indicate or imply the relative importance or number of the indicated devices, elements, or components. "plurality" means two or more unless otherwise specified.

The embodiment of the application provides a nucleic acid detection device which can be applied to small-scale outpatients, fever outpatients, and virulent infectious disease detection and other scenes; according to the nucleic acid detection device, the magnetic frame assembly, the extraction heating assembly, the PCR module, the driving module, the injector assembly and the ventilation and filtration system are assembled together, and after consumables such as a PCR tube, a reagent bottle and a gun head are placed, a detection process can be independently and fully automatically completed through one device without manual carrying; moreover, by arranging the ventilation and filtration system, biological cross contamination and biological safety hazards are avoided. The nucleic acid detection device has small volume and can realize the technical effect of improving the detection efficiency.

Referring to fig. 1, fig. 1 is a schematic diagram illustrating an exploded structure of a nucleic acid detecting apparatus according to an embodiment of the present disclosure, the nucleic acid detecting apparatus includes a base plate 10, a housing 11, a magnetic rack assembly 20, an extraction heating assembly 30, a PCR module 40, a driving module 50, an injector assembly 60, and a ventilation and filtration system 70.

Illustratively, the magnetic rack assembly 20 is mounted on the base plate 10, the magnetic rack assembly 20 includes a magnetic rod 21 and a first driving mechanism 22, the first driving mechanism 22 drives the magnetic rod 21 to move along a preset direction, the magnetic rod 21 is disposed at one side of the test agent rack, and the test agent rack is provided with magnetic beads.

Illustratively, the first drive mechanism 22 may be a linear motor; the first driving mechanism 22 drives the magnetic bar 21 to move the magnetic bar 21 up and down: when the first driving mechanism 22 drives the magnetic rod to ascend, the magnetic rod is close to the reagent shelf to be tested, magnetic beads are arranged in the reagent shelf to be tested, the magnetic rod 21 has magnetic force, the magnetic rod 21 gathers the magnetic beads in the reagent shelf to be tested, when the first driving mechanism 22 drives the magnetic rod 21 to descend, the magnetic rod 21 is far away from the reagent shelf to be tested, the magnetic beads are sucked through the injector component 60, and therefore the magnetic beads are scattered, and liquid in the reagent shelf to be tested is uniformly mixed.

Illustratively, the nucleic acid detecting device further comprises a middle fixing plate 12, a ground pin 13, a power supply 14 and a bracket 15; the ground feet 13 are assembled below the bottom plate and play a supporting role; the power supply 14 is assembled on the bottom plate and supplies power to the whole machine; the bracket 15 is assembled on the bottom plate and plays a role of supporting; the middle fixing plate 12 is assembled on the bracket and plays a supporting role.

Illustratively, the extraction heating assembly 30 is mounted to the base plate 10.

Illustratively, the extraction heating assembly 30 is disposed on the middle fixing plate 12, and the bead wells in the test agent rack can be heated by the extraction heating assembly 30 in a manner of heating the electric heating film at room temperature to 98 ℃.

Illustratively, the PCR module 40 is mounted to the base plate 10, and the extraction heating assembly 30 is mounted in mating relation with the PCR module 40.

Illustratively, the driving module 50 includes an X-axis module 51, a Y-axis module 52 and a Z-axis module 53, the X-axis module 51 is mounted on the base plate 10, the Y-axis module 52 is mounted on the X-axis module 51, the X-axis module 51 drives the Y-axis module 52 to move in the left-right direction, the Z-axis module 53 is mounted on the Y-axis module 52, and the Y-axis module 52 drives the Z-axis module 53 to move back and forth.

Illustratively, the ventilation filter system 70 is mounted inside the housing.

In some embodiments, the nucleic acid detecting apparatus further comprises an ultraviolet lamp 16; after the experiment is over, the uv lamp 16 can be applied for sterilization by itself.

In some embodiments, the PCR module 40 is assembled on the middle fixing plate 12, the heating mode is TEC, and the temperature rising and falling rate can reach about 4 to 5 degrees, or can be constant; the PCR module 40 may be heated, or may be used to determine whether the PCR tube has a negative or positive result. The X-axis module 51 is assembled on the base plate 10 and can drive the Y-axis module 52 to move left and right.

In some embodiments, the middle barrier 112 is assembled to the column 5101 of the X-axis module 51 to provide front-to-back isolation; the back is an electric control area, and the front door 17 is a placing area of the to-be-tested agent rack. The Y-axis module 52 is assembled on the X-axis module 51, and the motor of the X-axis module 51 drives the Y-axis module 52 to move left and right; the mechanical arm of the Z-axis module 53 is assembled on the Y-axis module 52, and the motor of the Y-axis module 52 drives the mechanical arm of the Z-axis module 53 to move back and forth.

In some embodiments, the housing 11 is assembled to the base plate 10 and the ultraviolet lamp 16 is assembled inside the housing 11 to act as a virus killer.

In some embodiments, the nucleic acid detection device further comprises a front door 17, the vent filtration system 70 comprises a filtration system housing 71, a blower 72, a blower holder plate 73, operating zone HEPA air purifier filter cotton 74, a filtration system baffle 75; the filtration system housing 71 is assembled inside the right side of the housing 11, the blower 72 is assembled to the blower holder plate 73, and this assembly is assembled together inside the filtration system housing 71; the HEPA air purifier filter cotton 74 in the operation area is inserted into the filtering system shell 71, when the experiment is finished, the front door 17 is closed, the air blower 72 in the equipment is electrified, the air in the operation area can be exhausted from the filtering system shell 71 to the outside of the equipment through the HEPA air purifier filter cotton 74 in the operation area, and the HEPA air purifier filter cotton 74 in the operation area can realize the air purification effect after being verified by a manufacturer; the filtration system baffle 75 is matingly mounted with the filtration system housing 71.

In some embodiments, the nucleic acid detecting device further comprises a back cover 18, a back cover HEPA air purifier filter cotton 19, a back cover fan 110; the back cover HEPA air purifier filter cotton 19 is assembled to the back cover 18, and the back cover fan 110 is assembled to the back cover 18; when the experiment was performed, the front door 17 was closed, the back cover fan 110 was opened, and the air inside the apparatus was discharged to the inside of the apparatus through the back cover fan 110 and the back cover HEPA air cleaner filter cotton 19.

In some embodiments, the syringe assembly 60 is assembled to the X-axis module 51, the syringe assembly 60 is connected to the gun head rod through a silicone tube, when the syringe assembly 60 operates, the liquid is transferred by sucking and discharging air, when the syringe assembly sucks air, the gun head inserts into the liquid, the syringe assembly sucks air, negative pressure is formed in the whole silicone tube, the liquid is sucked into the gun head, and the volume of the liquid can be adjusted according to actual conditions.

In some embodiments, as shown in FIG. 1, the nucleic acid detecting apparatus further comprises a consumable rack plate assembly 111, a middle baffle 112, a sealing strip 113, a thermal cover plate 114, a display screen 115, a thermal cover assembly 116, a bottom fan 117, and a face plate 118.

Illustratively, the nucleic acid detecting device further comprises a bottle locking assembly 80; the bottle locking assembly 80 comprises a locking electric gripper 81 and a screwing-cover electric gripper 82, after the screwing-cover electric gripper 82 grips the reagent bottle to be detected, the reagent bottle to be detected is moved to the position of the locking electric gripper 81, the reagent bottle to be detected is locked through the locking electric gripper 81, and the reagent bottle to be detected is unscrewed through the screwing-cover electric gripper 82.

Illustratively, the locking and uncovering actions of the reagent bottle to be tested are realized through the mutual matching of the locking electric hand grip 81 and the cover screwing electric hand grip 82, so that the subsequent detection process is conveniently realized.

In some embodiments, the electric bottle-locking gripper 81 is assembled on the bottom plate 10, and after the electric bottle-capping gripper 82 grips the bottle, the electric bottle-locking gripper 81 moves to a proper position, the electric bottle-locking gripper 81 grips the bottle body, so that the bottle body does not rotate, the electric bottle-capping gripper 82 grips the bottle cap, and the electric bottle-capping gripper 82 starts to rotate, so that the cap of the bottle can be screwed off.

Referring to fig. 2 and fig. 3, fig. 2 is a schematic diagram of an explosion structure of a PCR module according to an embodiment of the present disclosure, and fig. 3 is a schematic diagram of a PCR module according to an embodiment of the present disclosure.

Illustratively, the PCR module 40 includes a circuit board 41, a PCR stem 42, a TEC mechanism 43, and a heat sink 44; the TEC mechanism 43 is mounted to the heat sink 44, and the surface of the TEC mechanism 43 is coated with heat conductive silicone grease; a PCR tube holder 42 is mounted to the upper surface of the TEC mechanism 43; the circuit board 41 is provided with an LED lamp 45 and a detection sensor 46, the LED lamp 45 emits a detection light beam to the PCR tube 47, the detection sensor 46 receives the detection light beam reflected by the PCR tube 47, and the detection sensor 46 identifies liquid information of the PCR tube 47 according to the detection light beam.

Optionally, the detection sensor 46 is a color sensor.

Exemplarily, the TEC mechanism 43 is assembled to the heat sink 44, the upper and lower surfaces of the TEC mechanism 43 are coated with thermal silicone grease, and the TEC mechanism 43 can realize control functions such as temperature rise, temperature reduction, and constant temperature;

the PCR tube seat 42 is assembled on the upper surface of the TEC mechanism 43, the material of the PCR tube seat 42 is metal, when the TEC mechanism 43 is heated, heat can be transferred to the PCR tube seat 42, the PCR tube seat 42 transfers the heat to the PCR tube 47, the PCR tube 47 is heated, and liquid in the PCR tube 47 can be heated;

when the liquid reacts, the LED lamp 45 emits light, the light beam passes through the PCR tube 47 and the liquid inside, and then the liquid inside the PCR tube 47 reflects the light beam back, and the color sensor receives the reflected light beam. When the liquid in the PCR tube 47 is inserted into the PCR tube seat 42, the liquid in the PCR tube 47 is in one color, the LED lamp 45 emits light, the light is transmitted to the liquid in the PCR tube 47 through the hole, the liquid in the PCR tube 47 also reflects the light to the color sensor, the color sensor senses that the initial color and the return color have color difference, whether the PCR tube 47 and the liquid exist at the position is judged, and meanwhile, the temperature is increased/decreased, the liquid in the PCR tube 47 also changes, the color difference is different, and the negative and positive of the liquid in the PCR tube 47 can be judged according to the color difference; optionally, the PCR module 40 also includes an LED indicator 48 and a wire cover 49, the LED indicator 48 displaying a different color for operator identification, the wire cover 49 being used to match the various components of the mounted PCR module 40.

Referring to fig. 4, fig. 4 is an exploded view of a magnetic rack assembly according to an embodiment of the present disclosure.

Exemplarily, the magnetic frame assembly 20 further includes a triangular fixing plate 23, a magnetic frame base plate 24, an optical axis base 25, an optical axis 26, a linear bearing 27, a driving mounting base 28 and a movable plate 29; the triangular fixing plate 23 is mounted to the magnetic frame base plate 24, the optical axis seat 25 is fixed to the magnetic frame base plate 24, and the optical axis 26 is mounted to the optical axis seat 25; the linear bearing 27 is fixedly mounted to the movable plate 29, the first drive mechanism 22 is mounted to the drive mount 28, and the drive mount 28 is mounted to the magnet frame base plate 24.

Illustratively, the magnetic rack assembly 20 further includes a first sensing piece 210, a first photoelectric switch 211, a photoelectric switch base 212, and a magnetic rod base 213; the first sensing plate 210 is mounted to the movable plate 29, the first photoelectric switch 211 is mounted to the photoelectric switch base 212, the photoelectric switch base 212 is mounted to the magnetic stand bottom plate 24, the magnetic rod 21 is mounted to the magnetic rod base 213, and the magnetic rod base 213 is mounted to the movable plate 29, so that the movable plate 29 and the magnetic rod base 213 are driven by the first driving mechanism 22 to move the magnetic rod 21 up and down.

Illustratively, the first drive mechanism 22 is a linear motor, and the drive mounting mechanism is a linear motor mount; the triangular fixing plate 23 is assembled to the magnetic frame base plate 24, the optical axis seat 25 is fixed to the magnetic frame base plate 24, the optical axis 26 is assembled to the optical axis seat 25, and the optical axis 26 plays a role in guiding and sliding; the linear bearing 27 is fixed to the movable plate 29, the linear motor is assembled to the linear motor base, and the linear motor base is assembled to the magnetic frame bottom plate 24; the first sensing piece 210 is assembled to the movable plate 29, the photoelectric switch 211 is assembled to the photoelectric switch base 212, and the photoelectric switch base 212 is assembled to the magnetic stand bottom plate 24; the magnetic rod 21 is assembled to the magnetic rod seat 213, and the magnetic rod seat 213 is assembled to the movable plate 29; when the linear motor rotates, the movable plate 29, the magnetic rod seat 213 and the magnetic rod 21 are driven to move up and down; when the magnetic rod 21 is close to the magnetic beads of the reagent rack to be tested, the magnetic rod is attracted, and when the magnetic rod 21 is away, the magnetic rod is not attracted.

Please refer to fig. 5, fig. 5 is an exploded view of a Z-axis module according to an embodiment of the present disclosure.

Illustratively, the Z-axis module 53 includes a guide rail 54, a Z-axis base plate 55, a screw-capping electric gripper moving plate 56, a gun head moving plate 57, a guide rail slider 58, a gun head linear motor 59, and a screw-capping electric gripper linear motor 510; the guide rail 54 is mounted to the Z-axis base plate 55; the screwing cap electric gripper moving plate 56 is mounted to the guide rail sliding block 58, the gun head moving plate 57 is mounted to the guide rail sliding block 58, and the gun head linear motor 59 and the screwing cap electric gripper linear motor 510 are respectively mounted to the linear mounting base 516.

Exemplarily, the nucleic acid detecting apparatus further includes an X-axis sensing piece 511, a second sensing piece 512, a lance tip link 513, a lance tip rod 514, and a needle release plate 515; the X-axis induction sheet 511 is installed on the linear motor base, and the second induction sheet 512 is installed on the gun head movable plate 57 and the screwing cover electric hand grip movable plate 56; the lance tip link 513 is mounted to the lance tip rod 514, and the lance tip rod 514 is mounted to the lance tip movable plate 56; the stripper plate 515 is mounted to the Z-axis base plate 55.

Optionally, as shown in fig. 5, the Z-axis module 53 further includes a guide sleeve cover plate 520, a guide sleeve 521, and a stop block 522.

Illustratively, the guide rail 54 is assembled to the Z-axis base plate 55, the guide rail 54 serving a guiding function; the screwing cap electric gripper moving plate 56 is assembled to the guide rail sliding block 58, the gun head moving plate 57 is assembled to the guide rail sliding block 58, and the gun head linear motor 59 and the screwing cap electric gripper linear motor 510 are assembled to the linear mounting base 516 and serve as fixed motors; the X-axis sensing piece 511 is assembled to the linear mount 516. The second sensing piece 512 is assembled to the gun head movable plate 57 and the screw cap electric hand grip movable plate 56. The third photoelectric switch 517 is assembled to the Z-axis base plate 55. The electronic tongs 82 of screw-on cap assembles to electronic tongs fly leaf 56 of screw-on cap, and the effect of electronic tongs 82 of screw-on cap is snatched reagent bottle 519, moves to the electronic tongs 81 of bottle locking along XYZ axle, removes reagent bottle 519, twists off the reagent bottle lid. Gun head link 513 is assembled to gun head rod 514 and this assembly acts to telescope gun head 518 such that gun head link 513 is in a tight fit with gun head 518. The gun head rod is assembled to the gun head movable plate and moves up and down. The stripper plate 515 is assembled to the Z-axis base plate 55 and functions to remove the lance head 518 when the linear motor of the lance head 518 is moved upward. The assembling motion process comprises the steps that when an electric bottle-capping gripper moves to a reagent bottle position along an XYZ axis, the electric bottle-capping gripper is opened to grip a reagent bottle, then the electric bottle-capping gripper moves to the bottle-locking gripper position along the XYZ axis, the bottle-locking gripper is opened, the reagent bottle is placed into the bottle-locking gripper, then the bottle-locking gripper locks a reagent bottle body, the electric bottle-capping gripper rotates to unscrew a reagent bottle cap, then the reagent bottle cap is moved away along the XZY axis, at the moment, a gun head mechanical arm moves to sleeve a gun head, the gun head moves to the opened reagent bottle along the XYZ axis, a syringe assembly operates to suck liquid inside, then the gun head mechanical arm moves along the XYZ axis to a first reagent holder magnetic bead hole, the syringe assembly operates to discharge the liquid of the gun head, the gun head mechanical arm moves back to an initial position along the XYZ axis to retreat the gun head, then the electric gripper moves to cover the reagent bottle, the reagent bottle is covered, the reagent bottle is opened, the electric gripper locks the reagent bottle, the reagent bottle and the reagent bottle cap moves to the initial reagent holder position, and the reagent bottle-capping gripper completes the reagent bottle-capping gripper motion process. The device can make 16 copies at a time, and 16 cycles are required.

Referring to fig. 6, fig. 6 is a schematic view of an explosion mechanism of the reagent rack assembly according to the embodiment of the present application.

Illustratively, the nucleic acid detecting device further comprises a reagent rack assembly 90, wherein the reagent rack assembly 90 comprises a pressing bar 91, a first support plate 92, a second support plate 93, a reagent rack support plate 94 and a reagent bottle support plate 95; the hold down bar 91 is mounted to the reagent rack support plate 94; the first support plate 92 and the second support plate 93 are mounted to the reagent rack support plate 94, respectively; the reagent bottle support plate 95 is mounted to the reagent rack support plate 94.

Illustratively, the bead 91 is assembled to the reagent rack support plate 94, which acts as a stop. The first support plate 92 and the second support plate 93 are assembled to the reagent rack support plate to play a supporting role; optionally, the front support plate 96 is assembled to the reagent rack support plate, which acts as a support; the reagent bottle support plate 95 is assembled to the reagent rack support plate 94 and functions to support the limiting reagent bottle 519; the tip 98 is inserted into the tip hole of the reagent rack 97, the PCR tube 47 is inserted into the reagent rack support plate 94, the other components of the reagent rack assembly 90 are assembled into the reagent rack support plate 94, and the whole reagent rack assembly 90 is put into the panel 118 of the whole machine.

Referring to fig. 7, fig. 7 is a schematic diagram of an exploded structure of an extraction heating element according to an embodiment of the present disclosure.

Illustratively, the extraction heating assembly 30 includes a first heating plate 31, a heating assembly base 32, a heating film 33, a platen 34, a temperature protector 35, and a temperature protector platen 36; the first heating plate 31 is mounted to the heating assembly base 32, and the heating film 33 is mounted to the first heating plate 31; a platen 34 is mounted to the first heating plate 31, and a temperature protector 35, a temperature protector platen 36 are mounted to the platen 34, respectively.

Illustratively, a first heater plate 31 is assembled to the heater assembly base 32, a heater film 33 is assembled to the first heater plate 31, a pressure plate 34 is assembled to the first heater plate 31, and a temperature protector 35 and a temperature protector pressure plate 36 are assembled to the pressure plate 34; the extraction heating component 30 is used for heating extraction, and the first heating plate 31 has a limiting effect on the reagent rack; the temperature protector 35 is used to prevent the temperature from exceeding 100 deg.c, and the temperature protector 35 will cut off the power supply to the heating film automatically and return to the initial state when the temperature is proper.

Referring to fig. 8, fig. 8 is a schematic diagram illustrating an exploded structure of a thermal cover assembly according to an embodiment of the present disclosure.

Illustratively, the nucleic acid detecting apparatus further comprises a heat shield assembly 100, the heat shield assembly 100 comprising a heat shield 1001, a second heating plate 1002, a sliding plate 1003, a sliding block 1004, a heat shield rail 1005, a second driving mechanism 1006, a second driving mount 1007, a third sensing piece 1008, and a second photoelectric switch 1009; the heat insulation plate 1001 is mounted to the second heating plate 1002; a sliding plate 1003 is mounted to the second heating plate 1002, a sliding block 1004 is mounted to a thermal cover rail 1005; the second driving mechanism 1006 is mounted to a second driving mounting base 1007 and is matched with a linear motor screw 1012; the thermal cover rail 1005 is fixed to the face plate 118, the slide block 1004 is fixed to the slide plate 1003, the third sensing tab 1008 is fixedly mounted to the slide plate 1003, and the second opto-electronic switch 1009 is fixed to the face plate 118.

Illustratively, as shown in fig. 8, a second heating film 1010 is assembled to a second heating plate 1002, the second heating film 1010 serving as a heating function, the heating plate transferring heat; an insulation plate 1001 is assembled to the second heating plate 1002, and the insulation plate 1001 is a non-metal material and plays a role of insulation. The sliding plate 1003 is assembled to the second heating plate 1002, the sliding block 1004 is assembled to the thermal cover rail 1005, and the sliding plate 1003 functions as a coupling and fixing. The linear motor is assembled to the motor base and functions as a transmission distance. The hot cover rail 1005 is fixed to the face plate 118 for guiding, the slide block 1004 is fixed to the slide plate 1003, the third sensing piece 1008 is fixed to the slide plate 1003, the third photoelectric switch 1009 is fixed to the face plate 118, the motor base is fixed to the face plate 118, and the linear motor nut 1011 is fixed to the slide block 1004. When the linear motor rotates to drive the sliding plate to move back and forth and move to the position above the PCR tube, the heating film stops heating, heat is transferred to the PCR tube through the heating plate, and the temperature of liquid in the PCR tube is ensured to be uniform up and down without steam.

In some embodiments, the nucleic acid detection device provided by the embodiment of the application can solve the problem that one device places consumable materials such as a reagent rack, a PCR (polymerase chain reaction) tube, a reagent bottle and a gun head in a reagent rack supporting plate, places the reagent rack supporting plate assembly in an equipment panel fixing area, then starts the device to complete the operation independently and automatically without manual carrying, and the device is provided with an ultraviolet lamp after the experiment is finished and can disinfect by using the ultraviolet lamp. This equipment has the air ventilation filtration system of oneself, has avoided biological cross contamination and biosafety harm, and when killing, X, Y, Z axle arm can reciprocate around controlling, and the inside air of abundant agitated vessel makes the inside killing of equipment more abundant, has guaranteed that exhaust air is safe harmless. The device is small, like two desktop computers, and can be used in small outpatients, fever outpatients, and highly contagious diseases. Quick detection within 30 minutes, sample pretreatment function, constant temperature amplification function and self-purification function, and is applied to small-size outpatient service, two fans at the back exhaust to form negative pressure, the circulation detects the function, the hot lid, pretreatment consumptive material centralized processing, constant temperature processing system, the easy plug of filtration system detects different colour discernments, and the transmission is fluorescence detection, amplification, negative pressure, and joint heat dissipation, backplate convulsions heat dissipation.

In all embodiments of the present application, the terms "large" and "small" are relative terms, and the terms "more" and "less" are relative terms, and the terms "upper" and "lower" are relative terms, and the description of these relative terms is not repeated herein.

It should be appreciated that reference throughout this specification to "in this embodiment," "in an embodiment of the present application," or "as an alternative implementation" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present application. Thus, the appearances of the phrases "in this embodiment," "in the examples of the present application," or "as an alternative embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments. Those skilled in the art should also appreciate that the embodiments described in this specification are exemplary embodiments in nature, and that acts and modules are not necessarily required to practice the invention.

In various embodiments of the present application, it should be understood that the size of the serial number of each process described above does not mean that the execution sequence is necessarily sequential, and the execution sequence of each process should be determined by its function and inherent logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

The above description is only for the specific embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present application, and shall be covered by the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims (10)

1. A nucleic acid detection device is characterized by comprising a bottom plate, a shell, a magnetic frame assembly, an extraction heating assembly, a PCR module, a driving module, an injector assembly and a ventilation and filtration system;

the magnetic frame assembly is arranged on the bottom plate and comprises a magnetic rod and a first driving mechanism, the first driving mechanism drives the magnetic rod to move along a preset direction, the magnetic rod is arranged on one side of the reagent frame to be tested, and magnetic beads are arranged in the reagent frame to be tested;

the extraction heating assembly is mounted on the bottom plate;

the PCR module is arranged on the bottom plate, and the extraction heating assembly is matched with the PCR module;

the driving module comprises an X-axis module, a Y-axis module and a Z-axis module, the X-axis module is installed on the bottom plate, the Y-axis module is installed on the X-axis module, the X-axis module drives the Y-axis module to move in the left-right direction, the Z-axis module is installed on the Y-axis module, and the Y-axis module drives the Z-axis module to move back and forth;

the ventilation filter system is mounted inside the housing.

2. The nucleic acid detecting apparatus according to claim 1, further comprising a bottle locking assembly;

the bottle locking assembly comprises a locking electric gripper and a screwing-cap electric gripper, the screwing-cap electric gripper moves the reagent bottle to be detected to the position of the locking electric gripper after gripping the reagent bottle to be detected, the reagent bottle to be detected is locked through the locking electric gripper, and the reagent bottle to be detected is unscrewed through the screwing-cap electric gripper.

3. The nucleic acid detecting device of claim 2, wherein the PCR module comprises a circuit board, a PCR tube holder, a TEC mechanism, and a heat sink;

the TEC mechanism is mounted to the heat sink, and the surface of the TEC mechanism is coated with heat-conducting silicone grease;

the PCR tube seat is arranged on the upper surface of the TEC mechanism;

the circuit board is provided with an LED lamp and a detection sensor, the LED lamp emits a detection light beam to the PCR tube, the detection sensor receives the detection light beam reflected by the PCR tube, and the detection sensor identifies the liquid information of the PCR tube according to the detection light beam.

4. The nucleic acid detecting device according to claim 2, wherein the rack assembly further comprises a triangular fixing plate, a rack base plate, a spindle base, a spindle axis, a linear bearing, a drive mounting base, and a movable plate;

the triangular fixing plate is mounted to a magnetic frame bottom plate, the optical axis seat is fixed to the magnetic frame bottom plate, and the optical axis is mounted to the optical axis seat;

the linear bearing is fixedly mounted to the movable plate, the first driving mechanism is mounted to the driving mounting seat, and the driving mounting seat is mounted to the magnetic frame bottom plate.

5. The nucleic acid detecting device according to claim 4, wherein the magnetic rack assembly further comprises a first sensor sheet, a first photoelectric switch, a photoelectric switch holder, and a magnetic rod holder;

the first induction sheet is installed to the movable plate, the first photoelectric switch is installed to the photoelectric switch base, the photoelectric switch base is installed to the magnetic frame bottom plate, the magnetic rod is installed to the magnetic rod base, the magnetic rod base is installed to the movable plate, and the movable plate and the magnetic rod base are driven by the first driving mechanism to enable the magnetic rod to move up and down.

6. The nucleic acid detection device according to claim 2, wherein the Z-axis module comprises a guide rail, a Z-axis bottom plate, a screw cap electric gripper movable plate, a gun head movable plate, a guide rail slider, a gun head linear motor and a screw cap electric gripper linear motor;

the guide rail is mounted to the Z-axis base plate;

the gun head movable plate is mounted to the guide rail slide block, and the gun head linear motor and the screwing cap electric gripper linear motor are respectively mounted to the linear mounting seat.

7. The nucleic acid detecting device according to claim 6, further comprising an X-axis sensor chip, a second sensor chip, a tip link, a tip rod, and a needle release plate;

the X-axis induction sheet is mounted to the linear motor base, and the second induction sheet is mounted to the gun head movable plate and the screwing cover electric gripper movable plate;

the gun head link is mounted to the gun head rod, and the gun head rod is mounted to the gun head movable plate;

the stripper plate is mounted to the Z-axis bed.

8. The nucleic acid detecting device according to claim 1, further comprising a reagent rack assembly including a bead, a first support plate, a second support plate, a reagent rack support plate, and a reagent bottle support plate;

the pressing strip is mounted to the reagent rack support plate;

the first support plate and the second support plate are respectively mounted to the reagent rack support plate;

the reagent bottle support plate is mounted to the reagent rack support plate.

9. The nucleic acid detecting apparatus according to claim 1, wherein the extraction heating unit includes a first heating plate, a heating unit base, a heating film, a pressing plate, a temperature protector, and a temperature protector pressing plate;

the first heater plate is mounted to the heater assembly base and the heater film is mounted to the first heater plate;

the clamp plate is installed to first hot plate, temperature protector, the temperature protector clamp plate is installed respectively to the clamp plate.

10. The nucleic acid detecting apparatus according to claim 1, further comprising a thermal cover assembly, the thermal cover assembly comprising a thermal shield, a second heating plate, a sliding block, a thermal cover rail, a second driving mechanism, a second driving mount, a third sensing piece, and a second photoelectric switch;

the heat insulation plate is mounted to the second heating plate;

the sliding plate is mounted to the second heating plate and sliding blocks are mounted to the hot cover rail;

the second drive mechanism is mounted to the second drive mount;

the hot cover rail is fixed to the panel, the sliding block is fixed to the sliding plate, the third sensing piece is fixedly mounted to the sliding plate, and the second photoelectric switch is fixed to the panel.

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