CN1875738A - A feed addictive, its preparation process and use - Google Patents
A feed addictive, its preparation process and use Download PDFInfo
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- CN1875738A CN1875738A CNA2005100118767A CN200510011876A CN1875738A CN 1875738 A CN1875738 A CN 1875738A CN A2005100118767 A CNA2005100118767 A CN A2005100118767A CN 200510011876 A CN200510011876 A CN 200510011876A CN 1875738 A CN1875738 A CN 1875738A
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- bacillus
- culture
- saccharomycete
- photosynthetic bacteria
- inoculated
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- 238000002360 preparation method Methods 0.000 title description 2
- 241000894006 Bacteria Species 0.000 claims abstract description 50
- 238000000855 fermentation Methods 0.000 claims abstract description 50
- 230000004151 fermentation Effects 0.000 claims abstract description 50
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 36
- 239000007788 liquid Substances 0.000 claims abstract description 30
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 30
- 241001465754 Metazoa Species 0.000 claims abstract description 15
- 239000002054 inoculum Substances 0.000 claims description 59
- 239000002609 medium Substances 0.000 claims description 48
- 239000001963 growth medium Substances 0.000 claims description 34
- 238000003756 stirring Methods 0.000 claims description 28
- 238000004519 manufacturing process Methods 0.000 claims description 24
- 238000005516 engineering process Methods 0.000 claims description 21
- 241000235342 Saccharomycetes Species 0.000 claims description 18
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 14
- 241000186046 Actinomyces Species 0.000 claims description 12
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 12
- 244000144977 poultry Species 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 8
- 239000003651 drinking water Substances 0.000 claims description 8
- 235000020188 drinking water Nutrition 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 6
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 6
- 230000007613 environmental effect Effects 0.000 claims description 6
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 6
- 241000190932 Rhodopseudomonas Species 0.000 claims description 4
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims description 4
- 241000187747 Streptomyces Species 0.000 claims description 4
- 241001655322 Streptomycetales Species 0.000 claims description 4
- 235000013599 spices Nutrition 0.000 claims description 4
- 239000012467 final product Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 2
- 239000000654 additive Substances 0.000 abstract description 8
- 230000000996 additive effect Effects 0.000 abstract description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 5
- 230000007423 decrease Effects 0.000 abstract description 5
- 235000013372 meat Nutrition 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 239000004310 lactic acid Substances 0.000 abstract description 4
- 235000014655 lactic acid Nutrition 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 52
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 48
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 42
- 239000001888 Peptone Substances 0.000 description 30
- 108010080698 Peptones Proteins 0.000 description 30
- 235000019319 peptone Nutrition 0.000 description 30
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 27
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 24
- 239000008103 glucose Substances 0.000 description 24
- 239000011780 sodium chloride Substances 0.000 description 24
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- 235000019341 magnesium sulphate Nutrition 0.000 description 21
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- 239000012138 yeast extract Substances 0.000 description 13
- 238000005286 illumination Methods 0.000 description 12
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 12
- 239000000047 product Substances 0.000 description 12
- 230000003203 everyday effect Effects 0.000 description 10
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 10
- 229920002472 Starch Polymers 0.000 description 9
- 230000002354 daily effect Effects 0.000 description 9
- 239000011790 ferrous sulphate Substances 0.000 description 9
- 235000003891 ferrous sulphate Nutrition 0.000 description 9
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 9
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 9
- 229940099596 manganese sulfate Drugs 0.000 description 9
- 239000011702 manganese sulphate Substances 0.000 description 9
- 235000007079 manganese sulphate Nutrition 0.000 description 9
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 9
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 9
- 229920000053 polysorbate 80 Polymers 0.000 description 9
- 239000011734 sodium Substances 0.000 description 9
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- 238000011534 incubation Methods 0.000 description 8
- 241000283690 Bos taurus Species 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 241000287828 Gallus gallus Species 0.000 description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 229960002413 ferric citrate Drugs 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
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- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 6
- 208000004396 mastitis Diseases 0.000 description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 6
- 239000004323 potassium nitrate Substances 0.000 description 6
- 235000010333 potassium nitrate Nutrition 0.000 description 6
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- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 6
- 229940038773 trisodium citrate Drugs 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 5
- 235000002595 Solanum tuberosum Nutrition 0.000 description 5
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- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 4
- VLSOAXRVHARBEQ-UHFFFAOYSA-N [4-fluoro-2-(hydroxymethyl)phenyl]methanol Chemical compound OCC1=CC=C(F)C=C1CO VLSOAXRVHARBEQ-UHFFFAOYSA-N 0.000 description 4
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- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000726221 Gemma Species 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 3
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 3
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- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
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- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
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- -1 citric acid diamines Chemical class 0.000 description 3
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- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 3
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provide a kind additive product technique and purpose that can improve the enviroment of inner animal house, improve the animal fecundation index, lag rate ;survival rate, improve feed conversion rate, improve product rate, decline death rate decrease attibiuo tics utilization, improve economical efficient, improve character of meat, egg, mild, and can use for fermenting ensilage feed and feed. The additive of the invention mix with the different ratio fermentation liquid, such as photosynthetic bacterinm, bacillus, saccharomycote, lactic acid bacteria, actimomycete .
Description
Technical field
The invention belongs to field of fodder, be specifically related to a kind of additive for microbe feedstuff and production technology thereof and purposes.
Background technology
China's biological feedstuff industry starting is late, and with the distance that developed country also differs greatly, feed addictive industrial standardization system is unsound, quality is uneven, the bacterial classification of the interpolation in the various additive for microbe feedstuff is single, and some biology feed additive use is cumbersome, needs secondary fermentation.
Biology feed additive of the present invention, technical merit is reached advanced world standards, is by multiple beneficial active microorganism process scientific matching, is composited jointly, has really reached pleiotropism, and living bacteria count is considerably beyond national standard.
Biology feed additive result of use among the present invention is more obvious; Use more convenient (directly drinking-water or spice are saved the labour); The scope more of acting on wide (remove drinking-water or the conventional feed China and foreign countries that are added on, also can be used to ferment ensilage and yellow storage feed have increased the utilization rate of straw greatly, solve the contradiction that people and animals divide grain, have reduced these raw materials simultaneously to pollution that environment caused); Consumption is few, and cost is low;
Biology feed additive among the present invention is applied in the processing of environment, can effectively eliminate environmental odors; This kind feed addictive does not contain antibiotic composition, and the quality that improves product is had very big effect, by Chinese organic products authentication.
Summary of the invention
The purpose of this invention is to provide a kind of environment that can improve in the fowl animal house, can improve livestock immunity again, improve fowl poultry pregnancy rate, farrowing rate, survival rate, improve food conversion ratio, improve output, reduce the death rate, reduce antibiotic use, increase economic efficiency, improve feed addictive and the production technology and the purposes of the quality of meat, eggs and milk.
The invention discloses a kind of feed addictive, it is characterized in that, is to be mixed in proportion by photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic zymocyte liquid.Preferably, each zymocyte liquid by volume usage ratio be: actinomyces 5%, photosynthetic bacteria 15%, bacillus 15%, saccharomycete 25%, Bacillus acidi lactici 40%.
The invention also discloses the production technology of described feed addictive, comprise step:
(1) respectively photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic female kind are inoculated in the one-level culture medium and cultivate, obtain first class inoculum;
(2) respectively photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic first class inoculum are inoculated in secondary medium and cultivate, obtain second class inoculum;
(3) respectively photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic second class inoculum are inoculated in fermentation medium and cultivate, obtain zymocyte liquid;
(4) mixing is promptly in proportion with each zymocyte liquid.
Preferably, described photosynthetic bacteria is swamp Rhodopseudomonas (Rhodopseudomonaspalustris); Described bacillus is bacillus subtilis (Bacillus subtilis); Described saccharomycete is brewer's yeast (Saccharomyces cerevisiae); Described Bacillus acidi lactici is lactobacillus acidophilus (Lactobacillus acidophilus); Described actinomyces are Jingyang streptomycete (Streptomyces jingyangesis).
Production technology of the present invention, preferably, wherein step (2), (3) photosynthetic bacteria are that the batch (-type) ventilation is cultivated; Bacillus is cultivated for ventilation; Saccharomycete is cultivated for ventilation; Bacillus acidi lactici is cultivated for leaving standstill stuffiness; Actinomyces are cultivated for ventilation.
Details are as follows for production technology of the present invention (Fig. 1):
The production of each zymocyte liquid:
The photosynthetic bacteria production technology:
One, culture medium:
The one-level culture medium:
MgSO
47H
2O 0.2g, (NH
4)
2SO
41.0g, NaHCO
35.0g, K
2HPO
40.5g, NaCl 0.2g, peptone 1.5g.
(NH wherein
4)
2SO
4Can use NH
4Cl replaces, MgSO
4The saturated MgCl of available 0.25ml
2Replace, during preparation each composition is dissolved in the 400ml water, use H
3PO
4And Na
2CO
3Transfer PH, add entry again to 1000ml, pH is 7.1-7.2, sterilizes 10 minutes for 115 ℃.
Secondary medium:
Sodium acetate 3g, NaCl 0.1g, (NH
4)
2SO
40.3g, MgSO
40.2g, KH
2PO
30.5g, K
2HPO
30.3g, ferric citrate 7ml, peptone 1.2g, water is settled to 1000ml.Use H
3PO
4And Na
2CO
3Transferring pH is 7.1～7.2.
Fermentation medium:
Sodium acetate 2.6g, NaCl 0.07g, (NH
4)
2SO
40.25g, MgSO
40.16g, KH
2PO
30.38g, K
2HPO
30.25g, ferric citrate 5ml, peptone 1.0g, water is settled to 1000ml, uses H
3PO
4And Na
2CO
3Transferring pH is 7.1～7.2.
Fermented and cultured is divided two stages:
The 100L seed tank culture; 2 tons of fermentation tank culture.
Two, inoculation
After culture medium prepares, should inoculate immediately.The inoculum concentration that the photosynthetic bacteria productivity is cultivated is than higher, be generally 20%～50%, be that bacterial classification amount and the ratio of newly joining the cultivation amount are 1: 4 (20%)～1: 1 (50%), and should not be lower than 20%, especially the inoculation total amount is supported in little air culture should be high, otherwise photosynthetic bacteria is difficult to have comparative advantage in nutrient solution.
Three, the required environmental condition of growing
1, culture medium: distilled water
2, acid-base value, pH value are best 8～8.5, and the adaptation pH scope of photosynthetic bacteria is between 6-10.
3, temperature is an optimum growth temperature with 28～36 ℃.
4, intensity of illumination is the best with 3000～4000 luxs (LX), and promptly per 25 kilograms of bacterium liquid need be with the incandescent lamp as light source that is equivalent to about 60 watts.
5, incubation time: 7-10 days
Four, cultivate management
1, stir or inflate: must stir or inflation in the photosynthetic bacteria incubation, its effect is that the photosynthetic bacteria come-up that helps to precipitate obtains illumination, keeps the good growth of bacterial cell.Shaking bottle anaerobic cultivation is to make the bacterial cell come-up with the method for manually shaking culture vessel, can add a small amount of bead before inoculation in culture vessel, is easy to stir bacterial cell when shaking; Stir with shaft during fermentation tank culture, shake at least every day or stir 3 times, regularly carry out.
2, regulate illuminance: cultivating photosynthetic bacteria need throw light on continuously, therefore in daily management work, should often adjust illuminance as requested.When rigidly connecting into bacterial classification because bacterial density is low, and intensity of illumination is adjusted to 3000lx, when middle and later periods bacterial growth breeding fast, the density height of bacterial cell, intensity of illumination should be brought up to 4000lx.
3, regulate temperature: in the incubation of photosynthetic bacteria, can effectively temperature be controlled under the optimum condition, it is optimal that yes.Temperature all is controlled at 32-36 ℃ in incubation.
4, the mensuration of acid-base value and adjustment: in order to prolong the exponential phase of growth of photosynthetic bacteria, improve the utilization rate and the yield of unit water body of culture medium, measuring and adjusting the pH value is important measures.The general acid-base value that adopts the way that adds acetic acid to reduce bacterium liquid in daily management work, must be measured the pH value of bacterium liquid every day, exceeds 8.5 when the pH value rises, and promptly adds acid and is adjusted to 7.5.
5, measure optical density (O.D.) value: be linear approximate relationship between OD value and the dry cell weight, this dependency relation is commonly used to measure the concentration of culture.At first, measure the OD value of bacterium liquid then, can roughly estimate the concentration of bacterial cell according to calibration curve according to the data of the measuring relevant canonical plotting that draws.OD value is used spectrophotometric determination always.In daily management mission,, can understand the growth and breeding situation of photosynthetic bacteria by measuring the optical density of bacterium liquid.
The bacillus production technology:
The one-level culture medium:
Beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, agar 2.0%, distilled water 1000ml, pH 7.0-7.2.
Or: beef extract 1.0%, peptone 1.0%, sodium chloride 0.5%, agar 2.0%, distilled water 1000ml, pH7.0～7.2.
Condition of culture: 30～32 ℃, 23～25 hours.
Secondary medium:
With the one-level culture medium.
The 250ml Kolle flask is adorned 65～70ml culture medium, and each bottle inclined-plane size should be consistent.
Condition of culture: 30-37 ℃, 2～3 days.
Fermentation medium:
Starch 0.3%, sucrose 0.5%, 30.8mg/Kg manganese sulfate solution 0.2%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%, dregs of beans 3.0%, water supplies 100%, pH7.0～7.5.
Or: soya-bean cake powder 3.0%, corn flour 2.0%, wheat bran 3.0%, sodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.03%, water supply 100%, pH7.0～7.5.
Fermented and cultured branch two-stage is carried out
1,100L seeding tank condition: press the fermentation medium batching; Volume is 59～61 liters after disappearing; Adjust pH 7.0～7.5; Temperature: 34～37 ℃, ventilating ratio 1: 0.3～0.5; Cultivation cycle 14～16 hours;
The omnidistance stirring.
2,2 tons of fermentation tank conditions: press the fermentation medium batching; Back 1.3～1.4 tons of volumes (cubic meter) disappear; Transfer pH 7.0～7.5; Temperature: 33～37 ℃, ventilating ratio 1: 0.3～0.5 adjusted to 0.6～0.8 after 6-8 hour; The omnidistance stirring is about rotating speed 200rpm/min; Cultivation cycle: be no more than 26 hours; Fermentation termination gemma ratio should reach more than 95%.
The saccharomycete production technology
The one-level culture medium:
A, PDA culture medium:
Potato juice 1000ml, glucose 20g, agar 20g.
Potato is soaked the method for making of juice: the 500g potato, and 1000ml water is used in the peeling stripping and slicing, boils soft; Use filtered through gauze, supply 1000ml water.
B, malt extract medium: the brewer's wort 1000ml of 12 Du Bolin (pol unit) adds 15-20g agar, dissolving back packing.
Condition of culture: 29～30 ℃ of temperature, 2～3 days time.
Secondary medium: yeast extract 1%, peptone 2%, glucose 2%, magnesium sulfate 0.06%, dipotassium hydrogen phosphate 0.25%, water supply 100%, pH6.0～6.3.
Condition of culture: 28～30 ℃ of temperature, rotating speed 150～160rpm/min, cultivation cycle 24～28 hours, terminal point pH about 6, inoculum concentration: 200/1000.
Fermentation medium:
Glucose 3%, peptone 0.3%, magnesium sulfate 0.04%, ammonium sulfate 0.3%, dipotassium hydrogen phosphate 0.2%, water supply 100%.
Fermented and cultured branch two-stage is carried out:
1, seed tank culture condition: back pH6.8～7.0 disappear; 28～30 ℃ of cultivation temperature; The omnidistance stirring; Ventilating ratio adjusts to 0.6～0.8 after 1: 0.3～0.5,6～8 hours, and about rotating speed 200rpm/min, cultivation cycle 24～28 hours is about terminal point pH5.5.
2,2 tons of fermentation tank culture conditions: back pH6.8～7.0 disappear; Volume is about 1.4 tons after disappearing; 28～30 ℃ of cultivation temperature; The omnidistance stirring; Ventilating ratio adjusts to 0.6～0.8 after 1: 0.3～0.5,6～8 hours, about rotating speed 220rpm/min, and cultivation cycle 30～36 hours; Terminal point pH about 4.
The lactic acid bacteria production technology:
The one-level culture medium:
A, MRS culture medium: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 10.0g, Tween-80 1.0g, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, trisodium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, L-cysteine hydrochloride 0.5g, agar 20g, distilled water are supplied 1000ml, pH 7.5.
B, degreasing milk medium: skimmed milk powder 11.0g, yeast extract 0.2g, agar 2g, distilled water are supplied 100ml, with before adding 0.27g L-cysteine hydrochloride (also can not adding).
Condition of culture: 38～40 ℃ of temperature, 1～2 day cycle.
Secondary medium:
Peptone 10g, beef extract 10g, yeast extract 5g, glucose 5g, Tween-80 1g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, citric acid diamines 2g, magnesium sulfate 0.2g, the violent 0.05g of sulfuric acid, distilled water are supplied 1000ml, pH 6.5～6.8.
Condition of culture: 38～40 ℃ of temperature, cultivation is left standstill in stuffiness, 24～36 hours cycles, inoculum concentration: 200/1000.
Fermentation medium:
Peptone 8.8g, beef extract 7.2g, yeast extract 4.1g, glucose 12g, Tween-80 1.0g, dipotassium hydrogen phosphate 1.6g, sodium acetate 3.8g, trisodium citrate 1.1g, magnesium sulfate 0.2g, manganese sulfate 0.04g, water 1000ml, pH7.3～7.5.
Fermented and cultured branch two-stage is carried out:
1, seed tank culture condition: 38～40 ℃ of cultivation temperature, the back pH6.8-7.0 that disappears, cultivation is left standstill in stuffiness, 18～22 hours cycles.
2,2 tons of fermentation tank culture conditions: 37～40 ℃ of cultivation temperature, the back pH 6.8-7.0 that disappears, volume is about 1.4 tons after disappearing, and cultivation is left standstill in stuffiness, and cycle 10-15 hour, below the terminal point pH4.0.
The actinomyces production technology:
The one-level culture medium:
The Gause I synthetic media: potassium nitrate 1g, soluble starch 20g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate 0.01g, agar 20g, distilled water are supplied 1000ml, pH 7.2～7.4.
Condition of culture: 27～29 ℃, 5～7 days.
Also available PDA culture medium.
Secondary medium: potassium nitrate 0.8g, soluble starch 8g, glucose 5g, yeast juice 5g, dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.2g, sodium chloride 0.4g, ferrous sulfate 0.05g, water are supplied 1000ml, pH 7.2～7.4.
Condition of culture: 27～29 ℃, 150～160rpm/min, 4～5 days.
Fermentation medium: glucose 1%, yeast juice 1%, dipotassium hydrogen phosphate 0.015%, sodium chloride 0.02%, ferrous sulfate 0.003%, water supply 100%, pH 7.2～7.5.
Fermented and cultured branch two-stage is carried out:
1, seed tank culture condition: 27～30 ℃, 160～170rpm/min, ventilating ratio adjust to 0.6～0.8,5～6 days after 1: 0.3～0.5,6～8 hours.
2,2 tons of fermentation tank culture conditions: 27～30 ℃, 160～180rpm/min, ventilating ratio are adjusted to 0.6～0.8,5～8 days after 1: 0.3～0.5,6～8 hours.
Mix finished product:
1, five kinds of bacterium liquid that ferment is carried out inspection by sampling respectively, as squeezing into fluid reservoir after qualified in proportion.(mixed proportion is: actinomyces 5%; Photosynthetic bacteria 15%; Bacillus 15%; Saccharomycete 25%; Bacillus acidi lactici 40%)
2, after being mixed, the bacterium liquid of fluid reservoir carries out inspection by sampling, as the qualified finished product that is.
The present invention further discloses described feed addictive nest house environmental applications in improving livestock rearing.
Environmental treatment: colony house is raiseeed preceding 3 days with 300 times of bacterium liquid sprinklings once input fowl, sprays once (annotate: the concentration multiple calculates with feed addictive bacterium liquid of the present invention, and bacterium liquid promptly refers to feed addictive of the present invention) every 10-15 days with 500 times of bacterium liquid afterwards.
The invention also discloses the application of described feed addictive, it is characterized in that, when the fowl poultry eats siccative, when drinking-water, add described feed addictive in proportion; During the edible damp spice of fowl poultry, in feed, add described feed addictive in proportion and stir and get final product.
Preferably, if fowl poultry edible be siccative, only need when drinking-water, to add the bacterium liquid of dilution: begin 1-3 days, and, used 1000 times drinking-water (notes: the concentration multiple calculates with bacterium liquid) afterwards with 300 times drinking-water; If what fowl poultry was edible is damp spice, only need in feed, to add bacterium liquid and stir and get final product, ratio is the same.
Feed addictive beneficial effect of the present invention is embodied in following several aspect:
One, improves the interior environment of fowl animal house
Environmental pollution is a great problem that modernized aquaculture runs into, and the foul smell in the fowl animal house can cause multiple disease, is directly threatening the health of fowl poultry.Adopt feed addictive biotechnology of the present invention, can will form the substance decomposition reduction such as ammonia, hydrogen sulfide, methyl mercaptan, trimethylamine of stench, thereby solve this difficult problem.
Two, improve the power of exempting from service, effectively prevent the generation of various diseases, improve pregnancy rate, farrowing rate, survival rate
The fowl poultry of adopting additive for microbe feedstuff technology of the present invention to feed, can make useful active microorganism in the enteron aisle of animal, form optimum microbiologic population, thereby suppress growing of harmful germ, animal gastrointestinal tract had tangible conditioning functions, improve the immunocompetence of animal, thereby reach prevent disease, (as: the comprehensive complication of pig, the various intestines problems of chicken, mammitis of ox and claw ill etc.).
Three, improve food conversion ratio, reduce feedstuff-meat ratio, feedstuff-egg ratio, reduce cost
Additive for microbe feedstuff of the present invention has very strong capacity of decomposition, can effectively decompose the available nutrient in the feed, improve protein utilization, can further improve the conversion ratio of feed, produce the animal UGF simultaneously, favourable animal faster growing makes meat material, egg material ratio, milk feed ratio obviously improve, thereby reduce cost.
Four, improve output, the reduction death rate, reduce the egg breakage rate
Five, reduce antibiotic use, increase economic efficiency, improve the quality of meat, eggs and milk
Raise with additive for microbe feedstuff of the present invention, reduce the use amount of antibiotics greatly, make that the product of producing is nontoxic, drug residue free, no hormone, improve the quality of product, meet the requirement of organic products fully.
Six, remove directly drinking-water or routine and be added on the feed China and foreign countries, also can be used to ferment ensilage and yellow storage feed have increased the utilization rate of straw greatly, solve the contradiction that people and animals divide grain, have reduced these raw materials simultaneously to pollution that environment caused.
Ensiling, yellow storage feed smell fragrant, soft and succulency, characteristics such as good palatability become one of herbvore domestic animal high-quality roughage such as cattle and sheep, and can receive the raising feed intake, increase the output of milk, improve the good result of the growth of livestock.
Description of drawings
Fig. 1: microbial inoculum technological process of production figure.
Fig. 2: milk cow demonstration output of milk comparison diagram.
The specific embodiment
Embodiment 1:
The production of each zymocyte liquid:
1, the female kind of photosynthetic bacteria swamp Rhodopseudomonas (Rhodopseudomonas palustris) is inoculated in one-level culture medium (MgSO
47H
2O 0.2g, (NH
4)
2SO
41.0g, NaHCO
35.0g, K
2HPO
40.5g, NaCl 0.2g, peptone 1.5g; Each composition is dissolved in the 400ml distilled water, uses H
3PO
4And Na
2CO
3Transfer pH 7.1, add distilled water again, sterilized 10 minutes for 115 ℃ to 1000ml.) on, 30 ℃ of temperature, incubation time is 7 days under the intensity of illumination 3000 lux conditions, obtains first class inoculum;
First class inoculum is inoculated in secondary medium (sodium acetate 3g, NaCl 0.1g, (NH
4)
2SO
40.3g, MgSO
40.2g, KH
2PO
30.5g, K
2HPO
30.3g, ferric citrate 7ml, peptone 1.2g, distilled water is settled to 1000ml, uses H
3PO
4And Na
2CO
3Transferring pH is 7.2.) on, in culture vessel, add a small amount of bead before the inoculation, 30 ℃ of temperature, cultivated 8 days under the intensity of illumination 4000 lux conditions, manually shake culture vessel and make the bacterial cell come-up, shake every day 3 times, regulate pH7.5 between culture period, obtain second class inoculum;
Second class inoculum is inoculated in the 100L seeding tank, and culture medium is fermentation medium (sodium acetate 2.6g, NaCl 0.07g, (NH in the seeding tank
4)
2SO
40.25g, MgSO
40.16g, KH
2PO
30.38g, K
2HPO
30.25g, ferric citrate 5ml, peptone 1.0g, water is settled to 1000ml, uses H
3PO
4And Na
2CO
3Transferring pH is 7.1～7.2.), inoculum concentration 25%, i.e. bacterial classification amount and newly to join the ratio of cultivating base unit weight be 1: 3, condition of culture: 28 ℃ of temperature, incubation time is 7 days under the intensity of illumination 3000 lux conditions, stirs with paddle during cultivation, stir every day 4 times, regularly carry out, regulate between culture period below the pH8.5;
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium), inoculum concentration is 20%, it is bacterial classification amount and newly to join the ratio of cultivating base unit weight be 1: 4, condition of culture: 30 ℃ of temperature, cultivated 8 days under the intensity of illumination 4000 lux conditions, stir with paddle during cultivation, stir every day 5 times, regularly carry out, regulate the pH value between culture period below 8.5.
2, (beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, distilled water 1000ml, pH 7.0 the female kind of bacillus bacillus subtilis (Bacillus subtilis) to be inoculated in the one-level culture medium.), condition of culture: 30 ℃, 23 hours, obtain first class inoculum;
First class inoculum is inoculated in secondary medium (beef extract 1.0%, peptone 1.0%, sodium chloride 0.5%, agar 2.0%, distilled water 1000ml, pH7.0.), 250ml Kolle flask dress 70ml culture medium, each bottle inclined-plane size should be consistent.Condition of culture: 30 ℃ of temperature 2 days, obtain second class inoculum;
Second class inoculum is inoculated in the 100L seeding tank: (starch 0.3%, sucrose 0.5%, 30.8mg/Kg manganese sulfate solution 0.2%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%, dregs of beans 3.0%, water supplies 100%, pH7.0 to press the fermentation medium batching.); Volume is 59 liters after disappearing; Ventilating ratio 1: 0.5, temperature: 37 ℃, the cycle surpasses 14 hours, the omnidistance stirring.
The seed tank culture thing is inoculated in 2 tons of fermentation tanks: (soya-bean cake powder 3.0%, corn flour 2.0%, wheat bran 3.0%, sodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.03%, water supply 100%, pH7.0) by the fermentation medium batching; Back 1.4 tons of volumes (cubic meter) disappear; Ventilating ratio 1: 0.3 adjusted to 0.6 after 6 hours; The omnidistance stirring is about rotating speed 200rpm/min; Cultivation cycle 26 hours; Fermentation termination gemma ratio reaches 95%.
3, the mother's kind with saccharomycete brewer's yeast (Saccharomyces cerevisiae Meyen etHansen) is inoculated in one-level culture medium (PDA culture medium: potato juice 1000ml, glucose 20g, agar 20g.), condition of culture: 29 ℃ of temperature, obtain first class inoculum at 2 days time;
First class inoculum is inoculated in secondary medium, and (yeast extract 1%, peptone 2%, glucose 2%, magnesium sulfate 0.06%, dipotassium hydrogen phosphate 0.25%, water supply 100%, pH6.0), condition of culture: 28 ℃ of temperature, rotating speed 150rpm/min, cultivation cycle 24 hours, terminal point pH about 6, inoculum concentration: 200/1000, obtain second class inoculum;
Second class inoculum is inoculated in adorns fermentation medium in the seeding tank (glucose 3%, peptone 0.3%, magnesium sulfate 0.04%, ammonium sulfate 0.3%, dipotassium hydrogen phosphate 0.2%, water supply 100%.), condition of culture: back pH6.8 disappears; 28 ℃ of cultivation temperature; The omnidistance stirring; Ventilating ratio 1: 0.5 adjusted to 0.6 after 8 hours, and about rotating speed 200rpm/min, cultivation cycle 24 hours is about terminal point pH5.5.
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium) condition of culture: back pH6.8 disappears; Volume is 1.4 tons after disappearing; 28 ℃ of cultivation temperature; The omnidistance stirring; Ventilating ratio 1: 0.3 adjusted to 0.6 after 6 hours, about rotating speed 220rpm/min, and cultivation cycle 30 hours; Terminal point pH about 4.
4, the mother of lactic acid bacteria lactobacillus acidophilus (Lactobacillus acidophilus) is planted be inoculated in the one-level culture medium (the MRS culture medium: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 10.0g, Tween-80 1.0g, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, trisodium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, L-cysteine hydrochloride 0.5g, agar 20g, distilled water are supplied 1000ml, pH 7.5.), condition of culture: 38 ℃ of temperature in 1 day cycle, obtain first class inoculum;
First class inoculum is inoculated in secondary medium, and (peptone 10g, beef extract 10g, yeast extract 5g, glucose 5g, Tween-80 1g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, citric acid diamines 2g, magnesium sulfate 0.2g, the violent 0.05g of sulfuric acid, distilled water are supplied 1000ml, pH 6.5.) condition of culture: 38 ℃ of temperature, cultivation is left standstill in stuffiness, 24 hours cycles, inoculum concentration: 200/1000, obtain second class inoculum;
Second class inoculum is inoculated in interior dress fermentation medium (peptone 8.8g, beef extract 7.2g, yeast extract 4.1g, glucose 12g, Tween-80 1.0g, dipotassium hydrogen phosphate 1.6g, sodium acetate 3.8g, trisodium citrate 1.1g, magnesium sulfate 0.2g, manganese sulfate 0.04g, water 1000ml, pH7.3.) seeding tank, condition of culture: 38 ℃ of cultivation temperature, disappear the back pH6.8, cultivation is left standstill in stuffiness, 18 hours cycles;
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium), condition of culture: 37 ℃ of cultivation temperature, the back pH 6.8 that disappears, volume is 1.4 tons after disappearing, and cultivation is left standstill in stuffiness, and 10 hours cycles are below the terminal point pH4.0.
5, the mother's kind with actinomyces Jingyang streptomycete (Streptomyces jingyangesis) is inoculated in one-level culture medium (Gause I synthetic media: potassium nitrate 1 gram, soluble starch 20 grams, dipotassium hydrogen phosphate 0.5 gram, magnesium sulfate 0.5 gram, sodium chloride 0.5 gram, ferrous sulfate 0.01 gram, agar 20 grams, distilled water is supplied 1000ml, pH7.2～7.4.), condition of culture: 27 ℃, 5 days.Obtain first class inoculum;
First class inoculum is inoculated in secondary medium, and (potassium nitrate 0.8 restrains, and soluble starch 8 restrains, and glucose 5 restrains, and yeast juice 5 restrains, and dipotassium hydrogen phosphate 0.3 restrains, and magnesium sulfate 0.2 restrains, and sodium chloride 0.4 restrains, and ferrous sulfate 0.05 restrains, and water is supplied 1000ml, pH7.2～7.4.) 27 ℃ of condition of culture, 150rpm/min 4 days, obtains second class inoculum;
With second class inoculum be inoculated in the dress fermentation medium (glucose 1%, yeast juice 1%, dipotassium hydrogen phosphate 0.015%, sodium chloride 0.02%, ferrous sulfate 0.003%, water supplies 100%, pH7.2～7.5.) seeding tank, condition of culture: 6.8,27 ℃ of the pH in back that disappear, 160rpm/min, ventilating ratio 1: 0.3 is adjusted to 0.6,5 day after 6 hours.
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium), condition of culture: pH is 6.8,27 ℃ after disappearing, 160rpm/min, and ventilating ratio 1: 0.3 is adjusted to 0.6,5 day after 6 hours.
Mix finished product:
1, five kinds of bacterium liquid that ferment is carried out inspection by sampling respectively, as squeezing into fluid reservoir after qualified in proportion.(mixed proportion is: actinomyces 5%; Photosynthetic bacteria 15%; Bacillus 15%; Saccharomycete 25%; Bacillus acidi lactici 40%)
2, after being mixed, the bacterium liquid of fluid reservoir carries out inspection by sampling, as the qualified finished product that is.
Embodiment 2:
The production of each zymocyte liquid:
1, the female kind of photosynthetic bacteria swamp Rhodopseudomonas (Rhodopseudomonas palustris) is inoculated in one-level culture medium (MgSO
47H
2O 0.2g, (NH
4)
2SO
41.0g, NaHCO
35.0g, K
2HPO
40.5g, NaCl 0.2g, peptone 1.5g; Each composition is dissolved in the 400ml distilled water, uses H
3PO
4And Na
2CO
3Transfer pH7.2, add distilled water again, sterilized 10 minutes for 115 ℃ to 1000ml.) on, 34 ℃ of temperature, incubation time is 7 days under the intensity of illumination 3000 lux conditions, obtains first class inoculum;
First class inoculum is inoculated in secondary medium (sodium acetate 3g, NaCl 0.1g, (NH
4)
2SO
40.3g, MgSO
40.2g, KH
2PO
30.5g, K
2HPO
30.3g, ferric citrate 7ml, peptone 1.2g, distilled water is settled to 1000ml, uses H
3PO
4And Na
2CO
3Transferring pH is 7.2.) on, in culture vessel, add a small amount of bead before the inoculation, 34 ℃ of temperature, cultivated 10 days under the intensity of illumination 4000 lux conditions, manually shake culture vessel and make the bacterial cell come-up, shake every day 3 times, regulate pH8.2 between culture period, obtain second class inoculum;
Second class inoculum is inoculated in the 100L seeding tank, and culture medium is fermentation medium (sodium acetate 2.6g, NaCl 0.07g, (NH in the seeding tank
4)
2SO
40.25g, MgSO
40.16g, KH
2PO
30.38g, K
2HPO
30.25g, ferric citrate 5ml, peptone 1.0g, water is settled to 1000ml, uses H
3PO
4And Na
2CO
3Transferring pH is 7.1～7.2.), inoculum concentration 25%, i.e. bacterial classification amount and newly to join the ratio of cultivating base unit weight be 1: 3, condition of culture: 32 ℃ of temperature, incubation time is 7 days under the intensity of illumination 3000 lux conditions, stirs with paddle during cultivation, stir every day 4 times, regularly carry out, regulate between culture period below the pH8.5;
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium), inoculum concentration is 20%, it is bacterial classification amount and newly to join the ratio of cultivating base unit weight be 1: 4, condition of culture: 36 ℃ of temperature, cultivated 10 days under the intensity of illumination 4000 lux conditions, stir with paddle during cultivation, stir every day 5 times, regularly carry out, regulate the pH value between culture period below 8.5.
2, the female kind of bacillus bacillus subtilis (Bacillus subtilis) is inoculated in one-level culture medium (beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, distilled water 1000ml, pH7.2.), condition of culture: 32 ℃, 25 hours, obtain first class inoculum;
First class inoculum is inoculated in secondary medium (beef extract 1.0%, peptone 1.0%, sodium chloride 0.5%, agar 2.0%, distilled water 1000ml, pH7.2.), 250ml Kolle flask dress 70ml culture medium, each bottle inclined-plane size should be consistent.Condition of culture: 37 ℃ of temperature 3 days, obtain second class inoculum;
Second class inoculum is inoculated in the 100L seeding tank: (starch 0.3%, sucrose 0.5%, 30.8mg/Kg manganese sulfate solution 0.2%, dipotassium hydrogen phosphate 0.3%, potassium dihydrogen phosphate 0.15%, dregs of beans 3.0%, water supplies 100%, pH7.5 to press the fermentation medium batching.); Volume is 61 liters after disappearing; Ventilating ratio 1: 0.5, temperature: 37 ℃, the cycle surpasses 16 hours, the omnidistance stirring.
The seed tank culture thing is inoculated in 2 tons of fermentation tanks: (soya-bean cake powder 3.0%, corn flour 2.0%, wheat bran 3.0%, sodium hydrogen phosphate 0.4%, potassium dihydrogen phosphate 0.03%, water supply 100%, pH7.5) by the fermentation medium batching; Back 1.4 tons of volumes (cubic meter) disappear; Ventilating ratio 1: 0.3 adjusted to 0.6 after 6 hours; The omnidistance stirring is about rotating speed 200rpm/min; Cultivation cycle 26 hours; Fermentation termination gemma ratio reaches 95%.
3, the mother's kind with saccharomycete brewer's yeast (Saccharomyces cerevisiae Meyen etHansen) is inoculated in one-level culture medium (PDA culture medium: potato juice 1000ml, glucose 20g, agar 20g.), condition of culture: 30 ℃ of temperature, obtain first class inoculum at 3 days time;
First class inoculum is inoculated in secondary medium, and (yeast extract 1%, peptone 2%, glucose 2%, magnesium sulfate 0.06%, dipotassium hydrogen phosphate 0.25%, water supply 100%, pH6.3), condition of culture: 30 ℃ of temperature, rotating speed 160rpm/min, cultivation cycle 28 hours, terminal point pH about 6, inoculum concentration: 200/1000, obtain second class inoculum;
Second class inoculum is inoculated in adorns fermentation medium in the seeding tank (glucose 3%, peptone 0.3%, magnesium sulfate 0.04%, ammonium sulfate 0.3%, dipotassium hydrogen phosphate 0.2%, water supply 100%.), condition of culture: back pH7.0 disappears; 30 ℃ of cultivation temperature; The omnidistance stirring; Ventilating ratio 1: 0.5 adjusted to 0.8 after 8 hours, and about rotating speed 200rpm/min, cultivation cycle 28 hours is about terminal point pH5.5.
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium) condition of culture: back pH7.0 disappears; Volume is 1.4 tons after disappearing; 30 ℃ of cultivation temperature; The omnidistance stirring; Ventilating ratio 1: 0.3 adjusted to 0.8 after 6 hours, about rotating speed 220rpm/min, and cultivation cycle 36 hours; Terminal point pH about 4.
4, the mother of lactic acid bacteria lactobacillus acidophilus (Lactobacillus acidophilus) is planted be inoculated in the one-level culture medium (the MRS culture medium: peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 10.0g, Tween-80 1.0g, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, trisodium citrate 2.0g, magnesium sulfate 0.2g, manganese sulfate 0.05g, L-cysteine hydrochloride 0.5g, agar 20g, distilled water are supplied 1000ml, pH 7.5.), condition of culture: 40 ℃ of temperature in 2 days cycles, obtain first class inoculum;
First class inoculum is inoculated in secondary medium, and (peptone 10g, beef extract 10g, yeast extract 5g, glucose 5g, Tween-80 1g, dipotassium hydrogen phosphate 2g, sodium acetate 5g, citric acid diamines 2g, magnesium sulfate 0.2g, the violent 0.05g of sulfuric acid, distilled water are supplied 1000ml, pH 6.8.) condition of culture: 40 ℃ of temperature, cultivation is left standstill in stuffiness, 36 hours cycles, inoculum concentration: 200/1000, obtain second class inoculum;
Second class inoculum is inoculated in interior fermentation medium (peptone 8.8g, beef extract 7.2g, yeast extract 4.1g, glucose 12g, Tween-80 1.0g, dipotassium hydrogen phosphate 1.6g, sodium acetate 3.8g, trisodium citrate 1.1g, magnesium sulfate 0.2g, manganese sulfate 0.04g, water 1000ml, pH7.3～7.5 adorned.) seeding tank, condition of culture: 40 ℃ of cultivation temperature, disappear the back pH7.0, cultivation is left standstill in stuffiness, 22 hours cycles;
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium), condition of culture: 40 ℃ of cultivation temperature, the back pH 7.0 that disappears, volume is 1.4 tons after disappearing, and cultivation is left standstill in stuffiness, and 15 hours cycles are below the terminal point pH4.0.
5, the mother's kind with actinomyces Jingyang streptomycete (Streptomyces jingyangesis) is inoculated in one-level culture medium (Gause I synthetic media: potassium nitrate 1 gram, soluble starch 20 grams, dipotassium hydrogen phosphate 0.5 gram, magnesium sulfate 0.5 gram, sodium chloride 0.5 gram, ferrous sulfate 0.01 gram, agar 20 grams, distilled water is supplied 1000ml, pH7.4.), condition of culture: 29 ℃, 7 days.Obtain first class inoculum;
First class inoculum is inoculated in secondary medium, and (potassium nitrate 0.8 restrains, and soluble starch 8 restrains, and glucose 5 restrains, and yeast juice 5 restrains, and dipotassium hydrogen phosphate 0.3 restrains, and magnesium sulfate 0.2 restrains, and sodium chloride 0.4 restrains, and ferrous sulfate 0.05 restrains, and water is supplied 1000ml, pH7.4.) condition of culture: 29 ℃, 160rpm/min 5 days, obtains second class inoculum;
With second class inoculum be inoculated in the dress fermentation medium (glucose 1%, yeast juice 1%, dipotassium hydrogen phosphate 0.015%, sodium chloride 0.02%, ferrous sulfate 0.003%, water supplies 100%, pH7.5.) seeding tank, condition of culture: 7.0,30 ℃ of the pH in back that disappear, 170rpm/min, ventilating ratio 1: 0.5 is adjusted to 0.8,6 day after 8 hours.
The seed tank culture thing is inoculated in 2 tons of fermentation tanks (interior dress fermentation medium), condition of culture: pH is 7.0,30 ℃ after disappearing, 180rpm/min, and ventilating ratio 1: 0.5 is adjusted to 0.8,8 day after 8 hours.
Mix finished product:
1, five kinds of bacterium liquid that ferment is carried out inspection by sampling respectively, as squeezing into fluid reservoir after qualified in proportion.(mixed proportion is: actinomyces 5%; Photosynthetic bacteria 15%; Bacillus 15%; Saccharomycete 25%; Bacillus acidi lactici 40%)
2, after being mixed, the bacterium liquid of fluid reservoir carries out inspection by sampling, as the qualified finished product that is.
Experimental example 1: feed addictive of the present invention improves fowl animal house environmental test
The situation unit that table 1 feed addictive of the present invention improves foul smell: mg/litre
| Pernicious gas | Before the processing | After the processing |
| Ammonia | 2.209 | 0.005 |
| Hydrogen sulfide | 1.850 | Can't check |
| Methyl mercaptan | 0.016 | Can't check |
| Trimethylamine | 0.035 | Can't check |
Experimental example 2: improve the power of exempting from service, effectively prevent the generation of various diseases, improve pregnancy rate, farrowing rate, survival rate
Carry out cad pig in March, 2005 on kind pig farm, east, the Shunyi District Chen Ge village, Beijing, relied the demonstration of pig, selected 40 deadlocks altogether, rely pig, be divided into 20/group at random, the result who demonstrated 60 days:
Stiff, bad pig is ecological deterioration, genetic disease, the bad reason formation of yellowish-white dysentery prevention, generally will account for about the 10%-15% of piglet, and the expert is decided to be comprehensive complication, and this boar dry-eating is not long.
Table 2 utilization feed addictive effect of the present invention: unit:, kilogram
| Group | Number | First starting weight | All the end is heavy | Total augment weight | Daily gain | The survival number | Survival rate |
| Demonstration (stiff bad pig) | 20 | 16.8 | 53.05 | 36.25 | 0.604 | 20 | 100％ |
| Contrast (stiff bad pig) | 20 | 16.9 | It is dead successively to fail to respond to any medical treatment with antibiotic etc | 0 | 0％ | ||
By demonstration, with deadlock, bad pig that this kind biology feed additive is raised, about 10 days, it is normal that stiff, bad pig begins to recover, and by 60 days demonstration, reached the requirement (is 0.605 kilogram with tire normal pig daily gain) of market pig.
If bring into use feed addictive of the present invention to feed, can reduce or stop deadlock, rely the generation of pig, and can improve pregnancy rate, farrowing rate, survival rate from boar.Litter size can improve 25.12%, produces the young number of living and can improve 29.17%, and birth weight can improve 21.56%, and nascent nest can improve 28.19%.
Table 3 feed addictive of the present invention is to the kill ratio of chicken colibacillosis, salmonella, coccidia
| The Escherichia coli kill ratio | The salmonella kill ratio | The coccidia kill ratio |
| 96％ | 94％ | 46％ |
Table 4 milk cow is used the concrete effect (San He cattle farm, Beijing) of feed addictive of the present invention
| Daily yielding raising rate | The mammitis slip | The claw ill slip |
| 8.2％-20％ | 45.3％-76.5％ | 40.4％-54.6％ |
Experimental example 3: the test of improving food conversion ratio
Table 5 feed addictive of the present invention is raised the contrast of 77 days effect of growing and fattening pigs
(Maoming City Dianbai County, Guangdong Province) weight: kilogram
| Group | Number | Equal starting weight | All the end is heavy | All weightening finishes always | Equal daily gain | Feedstuff-meat ratio |
| The demonstration group | 20 | 18.96 | 61.03 | 42.07 | 0.546 | 2.42 |
| Control group | 20 | 19.67 | 54.76 | 35.09 | 0.456 | 2.57 |
Data can draw daily gain and improved 19.7% from top form, and feedstuff-meat ratio has reduced by 5.83%.
Experimental example 4: improve output, reduce the death rate, reduce the test of egg breakage rate
Test in chicken farm, the Xia Ge village, Pinggu District, Beijing
Table 6 43 days correction data of feed addictive demonstration laying hen of the present invention
| Group | Number of elements | A day feed intake (g) | Day egg production (g) | Feedstuff-egg ratio | The death rate | The egg breakage rate |
| The demonstration group | 5526 | 142.03 | 59.31 | 2.4 | 0.8 | 0.08 |
| Control group | 4846 | 147.57 | 57.44 | 2.6 | 0.9 | 0.14 |
| Increase and decrease amount (demonstration-contrast) | -5.54 | 1.87 | -0.2 | -0.1 | -0.06 | |
| Gradient | -3.8％ | 3.3％ | -7.7％ | -11.1％ | -42.9％ |
Experimental example 5: reduce antibiotic use, improve the quality test of meat, eggs and milk
Carried out fryer demonstration in chicken farm, Lianshan District new worker street, Huludao City, Liaoning Province in April, 2005, and extract two cages at random out and do contrast, from buying chicken to delivering for sale totally 50 days, 7 yuan/kilogram of the market prices, correction data is as follows:
Table 7 uses the gain situation of feed addictive of the present invention
| Group | Number of elements | Input cost (with a calculating) | Dead number of elements | Deliver counterpoise for sale | Net profit | ||||
| The chicken cost | Antibiotic | Feed addictive | Feed | Cost accounting | |||||
| The demonstration group | 3500 | 2.6 | 0.26 | 14.3 | 17.16 | 57 | 3.55 | 7.69 | |
| Control group | 3500 | 2.6 | 0.53 | 14.85 | 17.98 | 181 | 3.20 | 4.42 | |
| Increase and decrease amount (demonstration-contrast) | -0.82 | -124 | 0.35 | 3.27 | |||||
| Gradient | -4.56％ | -68.51％ | 10.94％ | 73.98％ | |||||
Can be drawn by last table data analysis: the fryer death rate that the fryer that uses this feed addictive to raise is raised than tradition has reduced by 68.5%, and input cost has reduced by 4.56%, delivers counterpoise for sale to have improved 10.94%, and a net profit has increased by 73.98%.
The * 7.69=26476.67 unit of demonstration group total revenue=(3500-57), the * 4.42=14669.98 unit of control group total revenue=(3500-181), the demonstration group is overcharged 11806.69 yuan of benefits than control group.
Table 8 feed addictive of the present invention is fed the egg of laying hen production and the contrast of common egg
(data are detected at the quality inspection center):
| Group | Protein | Fat | Cholesterol (mg/100g) | Phosphatide (mg/100g) |
| The demonstration group | 12.1 | 8.33 | 217 | 495.4 |
| Control group | 12.7 | 11.1 | 585 | 140.0 |
| Increase and decrease amount (demonstration-contrast) | -0.6 | -2.77 | -368 | 355.4 |
| Gradient | -4.7％ | -24.95％ | -62.9％ | 253.86％ |
As can be seen from the above table, use the additive for microbe feedstuff technology to raise the egg that laying hen produces, quality has raising greatly, and the cholesterol of this egg is 217mg/100g, well below the cholesterol standard (300mg/100g) of outlet Japan, European countries; And to the human body useful phosphatide of brain particularly, the detected value of this egg is 495.4mg/100g, is higher than the market average level of 140.0mg/100g far away; And the content of fat also is lower than national average level.The nutritive value of this egg is higher than common egg far away, reaches the standard of organic food.
Experimental example 6: be used for ensiling and yellow storage, improve the output of milk, prolong the peak period of giving milk
Carried out the milk cow demonstration in July, 2004 in Fengtai, Beijing three and cattle farm, extracted 20 oxen at random out, demonstration and control group are respectively 10, demonstrate 53 days, remove 15 day laundering period of changing feed, on average survey milk once weekly, and survey altogether six times in back 38 days.
Average every ox output of milk unit of table 9: kilogram
| Date | The demonstration group | Control group | Increment | Growth rate | Remarks |
| Before the demonstration on July 21 | 18.48 | 18.66 | -0.18 | Data do not participate in contrast before the demonstration | |
| August 1 | 20.40 | 19.54 | 0.86 | 4.40％ | |
| August 8 | 20.90 | 19.00 | 1.90 | 10.00％ | |
| August 15 | 19.83 | 18.43 | 1.40 | 7.60％ | |
| August 24 | 20.60 | 16.53 | 4.07 | 24.62％ | The control group a head of cattle can not continue to give milk because of suffering from mammitis |
| September 3 | 17.40 | 10.90 | 6.50 | 59.63％ | Three oxen of control group suffer from mammitis |
| September 8 | 16.80 | 10.99 | 5.81 | 52.87％ | Three oxen of control group suffer from mammitis |
| Equal daily yielding | 19.32 | 15.90 | 3.42 | 21.51％ |
Analyze from the output of milk: calculate with per kilogram milk 2 yuans, every ox fecund every day milk is increased income 6.84 yuan, deducts 1.44 yuan of costs that use this product, and every ox increases by 5.4 yuan of incomes than control group every day.
Other need to prove during the demonstration that control group has 3 oxen to suffer from mammitis at different times, can not continue to give milk, and medical expense can increase cost; If use this feed addictive for a long time, the peak period of giving milk can prolong 10-15 days.
Claims (11)
1, a kind of feed addictive is characterized in that, is to be mixed in proportion by photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic zymocyte liquid.
According to the described feed addictive of claim 1, it is characterized in that 2, each zymocyte liquid usage ratio by volume is: actinomyces 5%, photosynthetic bacteria 15%, bacillus 15%, saccharomycete 25%, Bacillus acidi lactici 40%.
3, the production technology of a kind of claim 1 or 2 described feed addictives comprises step:
(1) respectively photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic female kind are inoculated in the one-level culture medium and cultivate, obtain first class inoculum;
(2) respectively photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic first class inoculum are inoculated in secondary medium and cultivate, obtain second class inoculum;
(3) respectively photosynthetic bacteria, bacillus, saccharomycete, Bacillus acidi lactici, actinomycetic second class inoculum are inoculated in fermentation medium and cultivate, obtain zymocyte liquid;
(4) mixing is promptly in proportion with each zymocyte liquid.
According to the described production technology of claim 3, it is characterized in that 4, described photosynthetic bacteria is swamp Rhodopseudomonas (Rhodopseudomonas palustris).
According to the described production technology of claim 3, it is characterized in that 5, described bacillus is bacillus subtilis (Bacillus subtilis).
According to the described production technology of claim 3, it is characterized in that 6, described saccharomycete is brewer's yeast (Saccharomyces cerevisiae).
According to the described production technology of claim 3, it is characterized in that 7, described Bacillus acidi lactici is lactobacillus acidophilus (Lactobacillus acidophilus).
According to the described production technology of claim 3, it is characterized in that 8, described actinomyces are Jingyang streptomycete (Streptomyces jingyangesis).
9, according to the described production technology of claim 3, wherein step (2), (3) photosynthetic bacteria are that the batch (-type) ventilation is cultivated; Bacillus is cultivated for ventilation; Saccharomycete is cultivated for ventilation; Bacillus acidi lactici is cultivated for leaving standstill stuffiness; Actinomyces are cultivated for ventilation.
10, claim 1 or 2 described feed addictives are improving fowl animal house environmental applications.
11, the application of claim 1 or 2 described feed addictives is characterized in that, when the fowl poultry eats siccative, adds described feed addictive in proportion when drinking-water; During the edible damp spice of fowl poultry, in feed, add described feed addictive in proportion and stir and get final product.
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