CN102409013B - Organic material inoculant and preparation method thereof - Google Patents

Organic material inoculant and preparation method thereof Download PDF

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CN102409013B
CN102409013B CN 201110333334 CN201110333334A CN102409013B CN 102409013 B CN102409013 B CN 102409013B CN 201110333334 CN201110333334 CN 201110333334 CN 201110333334 A CN201110333334 A CN 201110333334A CN 102409013 B CN102409013 B CN 102409013B
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rice bran
microbial inoculum
organic material
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CN102409013A (en
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蔡培元
蔡立新
朴春会
朱忠贵
史振侠
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Jiangsu budding matrix science and Technology Development Co Ltd
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ZHENJIANG PEILEI MATRIX TECHNOLOGY DEVELOPMENT Co Ltd
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Abstract

The invention discloses an organic material inoculant, which is a complex microbial community composed of actinomycetes, yeast, lactic acid bacteria, bacillus and photosynthetic bacteria; the effective active bacteria content is 0.5-100000000 unit/g, wherein 40-60% thereof is actinomycetes, the dosage form is powder; the particle diameter of the powder is less than or equal to 0.2 mm, the water content is less than or equal to 20%; the pH value range is 5.5-7.5; and the invention has wine yeast smell. The optimal combination in the complex microbial community is as follows: 50 parts of actinomycetes, 10 parts of yeast, 10 parts of lactic acid bacteria, 15 parts of bacillus and 15 parts of photosynthetic bacteria. The preparation process flow of the material inoculant comprises preparing culturing pot, preparing material, dosing material, loading, culturing and fermenting, drying, crushing, detecting, packaging and storing. The organic material inoculant of the invention is a special organic material inoculant for vinegar residue, cassava residue and other organic materials; the effective active bacteria content is high, the degrading and rotting speed of cellulose is fast. The invention has disadvantages of fast temperature rise, high temperature, strong adaptability and simple preparation; the use effect is good, and the invention is especially suitable for self-culturing and self-consumption in substrate production enterprise.

Description

A kind of organic material composting microbial inoculum and preparation method thereof
Technical field
The present invention relates to a kind of microbial fermentation decomposing microbial inoculum, particularly relate to decomposing microbial inoculum of a kind of organic materials and preparation method thereof, belong to the microbial fermentation technology field.
Background technology
The organic wastes such as the acid-sludge of the annual generation of the light industries such as China's food, pharmacy, extraction using alcohol enormous amount, the dregs of a decoction, manioc waste, these organic waste utilization ratios are not high, great majority are all processed as " rubbish ", not only contaminate environment but also cause the wasting of resources.Since some units utilized these organic wastes as the raw material production soilless culture substrate, acid-sludge, the dregs of a decoction, manioc waste etc. became the organic materials resource, yet need to process by fermentation maturity could be for the production of soilless culture substrate for these organic materialss.Normal fermentation is processed, and mainly adopts the natural packing fermentation, not only the composting time long, efficient is low, it is inhomogeneous to become thoroughly decomposed, and nutrient runs off in a large number.Chinese patent CN1291006C discloses a kind of microbial starter culture, this starter by aspergillus, the composition of the microorganisms such as the side spore is mould, the arteries and veins spore is mould, yeast, use this starter can improve ferment effect.But this starter is not clearly mainly for the treatment of organic wastes such as agricultural crop straw, feces of livestock and poultry with its fermentative processing acid-sludge, the dregs of a decoction, manioc waste texts.
Summary of the invention
The objective of the invention is to adopt the organic wastes such as acid-sludge, manioc waste for the present soilless culture substrate of China is raw material, the present situation that lacks special-purpose decomposing microbial inoculum discloses that a kind of temperature is fast, the height that heats up, strong adaptability, effective, organic materials specific complex microbiobacterial agent that making method is simple and easy to do and preparation method thereof.
Technical scheme of the present invention is achieved in the following ways:
A kind of organic material composting microbial inoculum, it is characterized in that: it is the complex microbial community that is formed by combining by actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium and indigenous bacterium, hundred million units/gram of effective active bacterial content 0.5-1, wherein actinomycetes account for 40-60%, this microbial inoculum formulation is pulvis, powder diameter≤0.2mm, water content≤20%, pH5.5-7.5 has the Qu Xiangqi flavor.
Preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the described complex microbial community.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is as follows:
1) prepare to cultivate utensil: prepares long 30-60cm, wide 20-30cm, dark utensils such as the some and atomizer of the plastic culture dish of 2.5-4cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator, prepare simultaneously 1 of clean culturing room; The utensils such as culturing room, vinyl disc, gauze need to produced disinfection the day before yesterday, and sterilization method can be the physical methods such as ultraviolet ray, ozone machine, disinfecting time 5-6 hour; Also can adopt 30-35% formaldehyde solution to add water 7-10 doubly or the chemical disinfection method of 10-20% bromogeramine, disinfecting time 12-24 hour.
2) get the raw materials ready: prepare rice bran or wheat bran as cultivating bed material, described rice bran or wheat bran requirement are fresh, dry, nothing is gone mouldy, fineness≤0.2mm, about water content 15%, also can replace part rice bran or wheat bran with dried acid-sludge powder, cassava ground-slag or straw powder, the need that bed material is too thick adopt pulverizer to be ground into the particle of fineness≤0.2mm; Make auxiliary material with sugar, vinegar, sugar can be brown sugar, sucrose, glucose, and vinegar can be aromatic vinegar, rice vinegar, mature vinegar etc.
3) batching: press the proportioning of bed material 100kg, the sugared 1kg such as rice bran, vinegar 0.5kg, bacterial classification 2kg, take by weighing first the 2-5kg rice bran, add water and stir and be adjusted to water content 38-42%, be uniformly mixed into bacterial classification again and be bacterium material A; The water that sugar, vinegar is added 2-5kg is configured to sugar-vinegar liquid B; To remain rice bran paves on concrete floor or plastic film, take by weighing needed water by the 38-42% water content, stir after adding B, evenly be sprayed on the rice bran with atomizer, stir while spraying, again A is sprinkled into, uniform stirring adopts sodium hydroxide or potassium primary phosphate to adjust the pH value to 5-7, forms compound C, stacked 1-2 hour, rice bran is fully absorbed water.The calculation formula of amount of water is: the gross weight (kilogram) of answering amount of water=[(matrix requires water content-rice bran water content) ÷ (1-matrix requires water content)] dried rice bran.It is 40 that matrix requires water content ± 2%.
4) sabot: C is packed in the vinyl disc, about the thickness 3cm of C, about interior thickness 2.5cm, cover moist gauze around the dish, guarantee that substratum is exposed; The vinyl disc that will install again C is " groined type " and is stacked on through in the culturing room that disinfects, height 6-8 layer, and in upper lower floor dish, insert respectively thermometer, indoor extension Hygrothermograph.
5) cultivation and fermentation: cultivation and fermentation management minute three phases, 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and material temperature is controlled at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, and need the upper lower burrs reversing of position this moment, guarantees that mycelia evenly grows; This stage must prevent that following three kinds of phenomenons from occuring, the first, and material temperature is too high and culturing room's humidity is too low in the dish, and the moisture evaporation is too many, causes the dry hide phenomenon; The second, early stage, mixture moisture was excessive and material temperature is too high or excessively low, the bacterium piece occurred to evacuate phenomenon; The 3rd, the vinyl disc non-uniform charging, charging tray extruding, culturing room are too airtight, expect that perhaps local superheating, moisture are excessive, produce and burn bacterium phenomenon (material brown color occurs and the vinic acid flavor is arranged).3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and material temperature no longer raises and when beginning to descend, in time dispel the heat, and allows material temperature be down to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter.
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, namely becomes the organic material composting microbial inoculum after D dries, pulverizes.
7) detect: every batch of microbial inoculum product all needs to detect, and the effective active bacterial content reaches hundred million units/gram of 0.5-1, fineness≤0.2mm, and water content≤20%, pH5.5-7.5 has the Qu Xiangqi flavor.
8) packing, preservation: adopt opaque plastics bag to pack, every bag of 0.5-2kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
Beneficial effect of the present invention:
1, organic material composting microbial inoculum of the present invention plays that temperature is fast, the height that heats up, strong adaptability, result of use be good.
2, organic material composting microbial inoculum of the present invention is the become thoroughly decomposed special-purpose decomposing microbial inoculum of fermentative production soilless culture substrate of organic waste for light industry such as acid-sludge, manioc wastes, and its microbe species is many, the effective active bacterial content high, and the cellulose degradation speed of becoming thoroughly decomposed is fast.
3, this quick rotting agent making method is simple and easy to do, is particularly suitable for matrix manufacturing enterprise from numerous personal.
Description of drawings
Fig. 1 is process flow sheet of the present invention.
Embodiment
Below in conjunction with specific embodiment, further illustrate the present invention, should understand this embodiment only is used for explanation the present invention and is not used in and limits the scope of the invention, after having read the present invention, those skilled in the art all fall within the application's claims limited range to the modification of the various equivalent form of values of the present invention.
Embodiment 1:
A kind of organic material composting microbial inoculum, it is the complex microbial community that is formed by combining by actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium, 0.8 hundred million units/gram of effective active bacterial content, wherein actinomycetes account for about 50%, this microbial inoculum formulation is pulvis, powder diameter≤0.2mm, water content≤20%, pH6.0 has the Qu Xiangqi flavor.
The complex microbial community combination is: 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is as follows:
1) prepare to cultivate utensil: prepares about long 50cm, about wide 30cm, dark some on the plastic culture dish about 3cm, reach the utensils such as atomizer, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator, prepare simultaneously 1 of clean culturing room.
2) get the raw materials ready: make to cultivate bed material with rice bran, the rice bran requirement is fresh, dry, nothing is gone mouldy fineness≤0.2mm, water content about 15%; Make auxiliary material with brown sugar, vinegar; Brown shorts must be pulverized.
3) batching: press the proportioning of rice bran 100kg, brown sugar 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing first the 4kg rice bran, add water and stir and to be adjusted to water content 40 left and right sides %, be uniformly mixed into bacterial classification again and be bacterium material A; Water with sugar, vinegar adding 4kg is configured to sugar-vinegar liquid B; To remain rice bran paves on concrete floor or plastic film, take by weighing required water of answering amount of water by 40% left and right sides water content, stir after adding B, evenly be sprayed on the rice bran with atomizer, stir while spraying, again A is sprinkled into, uniform stirring is adjusted pH value to 6.0, forms compound C, stacked 2 hours, rice bran is fully absorbed water;
4) sabot: C is packed in the vinyl disc, the thickness 3cm of C around the dish, interior thickness 2.5cm covers moist gauze, guarantees that substratum is exposed; The vinyl disc that will install again C is " groined type " and is stacked on through in the culturing room that disinfects, 7 layers of height, and in the layer dish of upper, middle and lower, insert respectively thermometer, indoor extension Hygrothermograph;
5) cultivation and fermentation: cultivation and fermentation management minute three phases, 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 92 left and right sides %, and material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and material temperature is controlled at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, and need the upper lower burrs reversing of position this moment, guarantees that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and material temperature no longer raises and when beginning to descend, in time dispel the heat, and allows material temperature be down to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter;
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, namely becomes the organic material composting microbial inoculum after D dries, pulverizes;
7) detect: every batch of microbial inoculum product all needs to detect, and examination criteria is: 0.8 hundred million units/gram of effective active bacterial content, and fineness≤0.2mm, water content≤20%, pH6.0 has the Qu Xiangqi flavor;
8) packing, preservation: adopt opaque plastics bag to pack, every bag of 1kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
Embodiment 2:
A kind of organic material composting microbial inoculum, it is the complex microbial community that is formed by combining by actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium and indigenous bacterium, 0.5 hundred million units/gram of effective active bacterial content, wherein actinomycetes account for more than 40%, this microbial inoculum formulation is pulvis, powder diameter≤0.2mm, water content≤20%, pH5.5 has the Qu Xiangqi flavor.
Preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the complex microbial community.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is as follows:
1) prepare to cultivate utensil: prepares long 30cm, wide 20cm, the deeply some and atomizer of the plastic culture dish of 2.5cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator, prepare simultaneously 1 of clean culturing room.
2) get the raw materials ready: prepare wheat bran as cultivating bed material, the wheat bran requirement is fresh, dry, nothing is gone mouldy fineness≤0.2mm, water content about 15%; Make auxiliary material with sugar, vinegar.
3) batching: press the proportioning of wheat bran 100kg, sugared 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing first the 2-5kg wheat bran, add water and stir and to be adjusted to water content about 38%, be uniformly mixed into bacterial classification again and be bacterium material A; The water that sugar, vinegar is added 2kg is configured to sugar-vinegar liquid B; To remain wheat bran and pave on plastic film, take by weighing needed water by 38% water content, stir behind the adding B, evenly be sprayed on the wheat bran with atomizer, stir while spraying, A is sprinkled into again, uniform stirring is adjusted pH value to 5.0, form culture medium C, stacked 1 hour, wheat bran is fully absorbed water.
4) sabot: C is packed in the vinyl disc, about the thickness 3cm of C, about interior thickness 2.5cm, cover moist gauze around the dish, guarantee that substratum is exposed; The vinyl disc that will install again C is " groined type " and is stacked on through in the culturing room that disinfects, 6 layers of height, and in upper lower floor dish, insert respectively thermometer, indoor extension Hygrothermograph.
5) cultivation and fermentation: cultivation and fermentation management minute three phases, 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and material temperature is controlled at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, and need the upper lower burrs reversing of position this moment, guarantees that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and material temperature no longer raises and when beginning to descend, in time dispel the heat, and allows material temperature be down to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter.
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, namely becomes the organic material composting microbial inoculum after D dries, pulverizes.
7) detect: every batch of microbial inoculum product all needs to detect, and examination criteria is: the effective active bacterial content is not less than 0.5 hundred million units/gram, fineness≤0.2mm, and water content≤20%, pH5.5 has the Qu Xiangqi flavor.
8) packing, preservation: adopt opaque plastics bag to pack, every bag of 0.5kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
Embodiment 3:
A kind of organic material composting microbial inoculum, it is the complex microbial community that is formed by combining by actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium and indigenous bacterium, 100,000,000 units/gram of effective active bacterial content, wherein actinomycetes account for 60%, this microbial inoculum formulation is pulvis, powder diameter≤0.2mm, water content≤20%, pH7.5 has the Qu Xiangqi flavor.
Preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the complex microbial community.
A kind of making method of organic material composting microbial inoculum, Fig. 1 is seen in its technical process, its concrete making step is as follows:
1) prepare to cultivate utensil: prepares long 60cm, wide 30cm, dark utensils such as the some and atomizer of the plastic culture dish of 4cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator, prepare simultaneously 1 of clean culturing room.
2) get the raw materials ready: as cultivating bed material, the rice bran requirement is fresh, dry, nothing is gone mouldy fineness≤0.2mm, water content about 15% with rice bran; Make auxiliary material with sucrose, aromatic vinegar.
3) batching: press the proportioning of rice bran 100kg, sugared 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing first the 5kg rice bran, add water and stir and to be adjusted to water content 38-42%, be uniformly mixed into bacterial classification again and be bacterium material A; The water that sugar, vinegar is added 2-5kg is configured to sugar-vinegar liquid B; To remain rice bran paves on concrete floor or plastic film, take by weighing needed water by the 38-42% water content, stir after adding B, evenly be sprayed on the rice bran with atomizer, stir while spraying, again A is sprinkled into, uniform stirring is adjusted the pH value to 5-7, forms the compound culture medium C, stacked 2 hours, rice bran is fully absorbed water.
4) sabot: C is packed in the vinyl disc, about the thickness 3cm of C, about interior thickness 2.5cm, cover moist gauze around the dish, guarantee that substratum is exposed; The vinyl disc that will install again C is " groined type " and is stacked on through in the culturing room that disinfects, 8 layers of height, and in upper lower floor dish, insert respectively thermometer, indoor extension Hygrothermograph.
5) cultivation and fermentation: cultivation and fermentation management minute three phases, 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and material temperature is controlled at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, and need the upper lower burrs reversing of position this moment, guarantees that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and material temperature no longer raises and when beginning to descend, in time dispel the heat, and allows material temperature be down to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, training in 7-8 days winter.
6) dry, pulverizing: culture medium C obtains mycoplasma D through after the above fermentation culture, namely becomes the organic material composting microbial inoculum after D dries, pulverizes.
7) detect: every batch of microbial inoculum product all needs to detect, and examination criteria is: 100,000,000 units/gram of effective active bacterial content, and fineness≤0.2mm, water content≤20%, pH7.5 has the Qu Xiangqi flavor.
8) packing, preservation: adopt opaque plastics bag to pack, every bag of 2kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
The decomposing agent that present embodiment is made, it is the become thoroughly decomposed special-purpose decomposing microbial inoculum of fermentative production soilless culture substrate of organic waste for light industry such as acid-sludge, manioc wastes, its microbe species is many, the effective active bacterial content high, reach hundred million units/gram of 0.5-1, the cellulose degradation speed of becoming thoroughly decomposed is fast, and the materials such as the acid-sludge that is used for becoming thoroughly decomposed play that temperature is fast, height, strong adaptability, effective heat up.And this quick rotting agent making method is simple and easy to do, is particularly suitable for soilless culture substrate manufacturing enterprise from numerous personal.

Claims (3)

1. organic material composting microbial inoculum, it is characterized in that: it is the complex microbial community that is combined by actinomycetes, yeast, milk-acid bacteria, genus bacillus, photosynthetic bacterium, hundred million units/gram of effective active bacterial content 0.5-1, wherein actinomycetes account for 40-60%, this microbial inoculum formulation is pulvis, powder diameter≤0.2mm, water content≤20%, pH5.5-7.5 has the Qu Xiangqi flavor.
2. a kind of organic material composting microbial inoculum according to claim 1, it is characterized in that: preferred combination is 50 parts in actinomycetes, 10 parts in yeast, 10 parts of milk-acid bacterias, 15 parts of genus bacillus, 15 parts of photosynthetic bacteriums in the described complex microbial community.
3. the making method of the described organic material composting microbial inoculum of claim 1 is characterized in that: according to the following steps operation:
1) prepare to cultivate utensil: prepares long 30-60cm, wide 20-30cm, the deeply some and atomizer of the plastic culture dish of 2.5-4cm, weighing balance, plastic tank, plastic film, packing bag, gauze, Hygrothermograph, pH determinator;
2) get the raw materials ready: prepare rice bran or wheat bran as cultivating bed material, described rice bran or wheat bran requirement are fresh, dry, nothing is gone mouldy fineness≤0.2mm; Make auxiliary material with sugar, vinegar, described sugar can be brown sugar, sucrose, glucose;
3) batching: press the proportioning of rice bran 100kg, sugared 1kg, vinegar 0.5kg, bacterial classification 2kg, take by weighing first the 2-5kg rice bran, add water and stir and to be adjusted to water content 38-42%, be uniformly mixed into bacterial classification again and be bacterium material A; The water that sugar, vinegar is added 2-5kg is configured to sugar-vinegar liquid B; To remain rice bran paves on concrete floor or plastic film, take by weighing needed water by the 38-42% water content, stir after adding B, evenly be sprayed on the rice bran with atomizer, stir while spraying, again A is sprinkled into, uniform stirring is adjusted the pH value to 5-7, forms compound C, stacked 1-2 hour, rice bran is fully absorbed water;
4) sabot: C is packed in the vinyl disc, the thickness 3cm of C around the dish, interior thickness 2.5cm covers moist gauze, guarantees that substratum is exposed; The vinyl disc that will install again C is " groined type " and is stacked on through in the culturing room that disinfects, height 6-8 layer, and in upper lower floor dish, insert respectively thermometer, indoor extension Hygrothermograph;
5) cultivation and fermentation: cultivation and fermentation management minute three phases, 1. generate heat the stage: culturing room's temperature is controlled at 30-32 ℃, is not less than 25 ℃, and humidity is controlled at 90-95%, and material temperature will rise to more than 35 ℃ as early as possible in the dish; 2. hot stage: culturing room's temperature is controlled at 35-38 ℃, and material temperature is controlled at 40-45 ℃, and humidity is controlled at 85-90%; This stage cultivated through 48 hours, and charge level will present white hypha, and need the upper lower burrs reversing of position this moment, guarantees that mycelia evenly grows; 3. temperature-fall period: the culture material clumping of over grown hyphae is tight in dish, and material temperature no longer raises and when beginning to descend, in time dispel the heat, and allows material temperature be down to 33-34 ℃, and humidity is controlled at 70-75%; General summer was through 4-5 days, cultivation and fermentation end in 7-8 days winter;
6) dry, pulverizing: compound C obtains mycoplasma D through after the above fermentation culture, namely becomes the organic material composting microbial inoculum after D dries, pulverizes;
7) detect: every batch of microbial inoculum product all needs to detect, and the effective active bacterial content reaches hundred million units/gram of 0.5-1, fineness≤0.2mm, and water content≤20%, pH5.5-7.5 has the Qu Xiangqi flavor;
8) packing, preservation: adopt opaque plastics bag to pack, every bag of 0.5-2kg places drying, ventilation, shady and cool environment to preserve 6 months quality guaranteed perioves.
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CN1584009A (en) * 2004-06-02 2005-02-23 宁贵 Fermenting liquid for feed with microbiological colonies
CN1875738A (en) * 2005-06-06 2006-12-13 北京新纪元三色生态科技有限公司 A feed addictive, its preparation process and use
CN101606471A (en) * 2009-07-23 2009-12-23 安徽省丰田无籽西瓜技术有限公司 Seedling medium and preparation method that a kind of vinasse methane-sludge makes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1584009A (en) * 2004-06-02 2005-02-23 宁贵 Fermenting liquid for feed with microbiological colonies
CN1875738A (en) * 2005-06-06 2006-12-13 北京新纪元三色生态科技有限公司 A feed addictive, its preparation process and use
CN101606471A (en) * 2009-07-23 2009-12-23 安徽省丰田无籽西瓜技术有限公司 Seedling medium and preparation method that a kind of vinasse methane-sludge makes

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