CN102910988B

CN102910988B - Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer

Info

Publication number: CN102910988B
Application number: CN201210463592.1A
Authority: CN
Inventors: 刘彪
Original assignee: Pop Beijing Gram Eco Science Technology Co Ltd
Current assignee: Popek International Ecological Technology (Beijing) Co. Ltd.
Priority date: 2012-11-18
Filing date: 2012-11-18
Publication date: 2014-08-20
Anticipated expiration: 2032-11-18
Also published as: CN102910988A

Abstract

The invention discloses a compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and a preparation method of the compound microorganism liquid bacterial fertilizer and belongs to the technical field of microorganisms. The bacterial fertilizer comprises raw materials of, by weight, 10-20 parts of paprika powders, 10-20 parts of chilli powders, 5-15 parts of ginkgo leaf powders, 5-15 parts of chinaberry powders, 5-15 parts of lightyellow sophora root powders, 20-40 parts of mashed garlic, 40-80 parts of molasses, 4-8 parts of soybean meal, 5-15 parts of calcium sulfate, 5-15 parts of magnesium chloride, 5-10 parts of salt, 5-15 parts of photosynthetic bacterium strains, 5-15 parts of saccharomycete strains, 5-15 parts of lactic acid bacterium strains, 5-15 parts of streptomyces jingyangensis strains, 5-15 parts of bacillus subtilis strains and 800 parts of water. According to the compound microorganism liquid bacterial fertilizer, probiotic floras which are screened and are capable of coexisting are used for fermenting plant leaching agents of pepper, chilli, ginkgo leaves, chinaberry, lightyellow sophora roots and garlic, the plant leaching agents are capable of expelling and killing the insect pests and harmful bacteria, the compound microorganism liquid bacterial fertilizer has the advantages of being high in content of live bacteria of probiotics, high in activities, high in stability, small in using amount of plant materials, fast in acting effects, non-toxic and environment-friendly, and has wide application prospects in agricultural planting.

Description

A kind of complex microorganism liquid bacterial fertilizer of preventing and treating plant pest and preparation method thereof

Technical field

The invention belongs to microbial fertilizer technical field, be specifically related to one and can prevent and treat plant pest, replenish the calcium, mend the multi-efficiency such as magnesium, fertilising complex microorganism liquid bacterial fertilizer and preparation method thereof.

Background technology

Use unreasonably for a long time chemical fertilizer, agricultural chemicals, cause the wasting of resources and production cost to increase, severe environmental pollution, agricultural product quality declines.The green barrier of international agriculture trade is that export of farm produce difficulty increases, and peasant faces the severe challenge that Higher output is not accompanied by a higher income.In recent years, along with the raising of environmental protection and health perception, nationwide has started " green food " heat.Meanwhile, along with developing rapidly of foreign trade, also grow with each passing day to the demand of pollution-free food in world market.Some developed countries, in order to protect the agricultural from state, take Green Trade Barrier.A large amount of agricultural-food that use chemical fertilizer and agricultural chemicals are kept outside of the door.Therefore,, in agriculture production, there is Important Economic benefit, environmental benefit and social benefit be to open up new microorganism medicine fertilizer even not use chemical fertilizer and agricultural chemicals to reduce, produce the agricultural-food of high yield and high quality, improve international competitiveness, promote the Sustainable development of rural economy.But biological pesticide sterilant and the microbial-bacterial fertilizer of the development and production of Present Domestic institute can improve the immunizing power of plant, prevention physiological barrier, to reach the object of stable yield and high quality.But, promote in growth can not with chemical fertilizer comparison, can not be directly and agricultural chemicals comparison aspect curing the disease at desinsection.Before not entering agricultural modernization, the example that China also has a large amount of use vegetable Chinese herbal medicine materials to carry out diseases prevention insect protected, but there is the shortcomings such as vegetable Chinese herbal medicine material usage is large, and effect is single, and the time length is short.

Summary of the invention

The object of the invention is for the deficiencies in the prior art, biotechnology and Chinese Chinese traditional herbs technology are combined, provide that a kind of probiotics viable bacteria content is high, vegetable Chinese herbal medicine material usage is few, activity is high, stability is high, action effect is fast, there is kill harmful bacterium, kill disease and insect, replenish the calcium, mend the complex microorganism liquid bacterial fertilizer of the multi-efficiencies such as magnesium, fertilising, rehabilitating soil, volume increase.Another object of the present invention is to provide the preparation method of this complex microorganism liquid bacterial fertilizer.

To achieve these goals, the technical scheme that the present invention adopts is: a kind of complex microorganism liquid bacterial fertilizer of preventing and treating plant pest, it is characterized in that the raw material of preparing this bacterial manure is by weight: zanthoxylum powder 10-20 part, red chilly powder 10-20 part, ginkgo leaf powder 0-5 part, China tree powder 0-5 part, kuh-seng powder 0-5 part, salt 5-10 part, mashed garlic 20-40 part, be greater than 6,000,000,000/milliliter photosynthetic bacterium 5-15 part, be greater than 500,000,000/ml yeast bacterium 5-15 part, be greater than 10,000,000,000/milliliter plant lactobacillus 5-15 part, be greater than 100,000,000/milliliter Jingyang streptomycete 5-15 part, be greater than 6,000,000,000/milliliter subtilis 5-15 part, molasses 40-80 part, analysis for soybean powder 4-8 part, calcium sulfate 5-15 part, magnesium chloride 5-15 part, 800 parts, water.

The preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest of the present invention:

(1), in fermentor tank, add successively: 800 parts of molasses 40-80 parts, analysis for soybean powder 4-8 part, calcium sulfate 5-15 part, magnesium chloride 5-15 part, zanthoxylum powder 10-20 part, red chilly powder 10-20 part, ginkgo leaf powder 5-15 part, China tree powder 5-15 part, kuh-seng powder 5-15 part, salt 5-10 part, water, heated and boiled 30 minutes, is cooled to 40 degree;

(2) the cooling fluid of, (1) producing in step adds mashed garlic 20-40 part, is greater than 6,000,000,000/milliliter photosynthetic bacterium 5-15 part, is greater than 500,000,000/ml yeast bacterium 5-15 part, is greater than 10,000,000,000/milliliter plant lactobacillus 5-15 part, is greater than 100,000,000/milliliter Jingyang streptomycete 5-15 part, is greater than 6,000,000,000/milliliter subtilis 5-15 part;

(3), 30-35 degree is cultivated 120-150 hour, stirring in every 10 hours 30 minutes;

, again at the static cultivation of 30-35 degree 120-150 hour, detect viable bacteria total content and be greater than 4,000,000,000/milliliter;

(5), emit four layers of filtered through gauze for bacterium liquid, packaging final prod.

Step (2) photosynthetic bacterium bacterial classification, yeast bacterial classification, lactic acid bacteria culturers, Jingyang streptomyces species, Bacillus subtilis strain can adopt prior art, the invention provides following methods.

1., the preparation of milk-acid bacteria liquid bacterial classification

Take out culture presevation pipe, draw flat board recover with MRS solid medium, 37 degree anaerobism are cultivated 48 hours.Under flat board, in 50 milliliters of MRS substratum of the single colony inoculation of picking, in incubator, 37 degree anaerobism are cultivated 24 hours.Seed adopts 5% inoculum size, is seeded in the large triangular flask of 10L, and 37 degree anaerobism are cultivated 20-28 hour, detect its bacterial concentration, and bacterial content exceedes 10,000,000,000/milliliter, can be used as the inoculation of lactobacillus suspension body bacterial classification.Wherein said MRS substratum: peptone 10g/L, yeast extract 5 g/L, extractum carnis 10 g/L, glucose 20 g/L, sodium-acetate 5 g/L, dibasic ammonium citrate 2 g/L, tween-80 1 ml/L, magnesium sulfate 0.6 g/L, manganous sulfate 0.05 g/L, dipotassium hydrogen phosphate 2 g/L, agar 20 g/L, calcium carbonate 20 g/L, add agar 20 g/L in solid medium.Wherein said milk-acid bacteria seed substratum: glucose 50 g/L, peptone 15 g/L, extractum carnis 7.5 g/L, sodium-chlor 1 g/L, manganous sulfate 0.01 g/L, magnesium sulfate 0. 2 g/L, sodium-acetate 0. 5 g/L, calcium carbonate 50 g/L, pH6.0.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.

2., the preparation of yeast liquid bacterial classification

Take out culture presevation pipe, draw flat board recover with PDA solid medium, 30 degree are cultivated 48 hours.Under flat board, in 50 milliliters of PDA substratum of the single colony inoculation of picking, in incubator, 30 degree concussions are cultivated 24 hours.Seed adopts 5% inoculum size, is seeded in the large triangular flask of 5L, and 20-28 hour is cultivated in 30 degree concussions, detects its bacterial concentration, and bacterial content exceedes 500,000,000/milliliter, can be used as the inoculation of yeast liquid body bacterial classification.Wherein said PDA substratum: potato 200g, sucrose 20g, water 1000mL, adds agar 20 g/L in solid medium.Wherein said yeast seed substratum: glucose 10 g/L, yeast extract paste 3 g/L, peptone 5 g/L, ammonium sulfate 5 g/L, magnesium sulfate 0.5 g/L.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.

3., the preparation of subtilis liquid spawn

Take out culture presevation pipe, draw flat board recover with LB solid medium, 37 degree are cultivated 48 hours.Under flat board, in 50 milliliters of LB substratum of the single colony inoculation of picking, in incubator, 37 degree concussions are cultivated 24 hours.Seed adopts 5% inoculum size, is seeded in the large triangular flask of 5L, and 20-28 hour is cultivated in 37 degree concussions, detects its bacterial concentration, and gemma content exceedes 6,000,000,000/milliliter, can be used as subtilis liquid-spawn inoculation.Wherein said LB substratum: peptone 5g, extractum carnis 3g, sodium-chlor 5g, water 1000mL, pH7.2, adds agar 2% in solid medium.Wherein said subtilis liquid spawn culture medium: glucose 10g/L, Semen Maydis powder 8 g/L, bean cake powder 25 g/L, magnesium sulfate 0.5 g/L, manganous sulfate 0.5 g/L, SODIUM PHOSPHATE, MONOBASIC 0.5 g/L, Sodium phosphate dibasic 2.3 g/L, pH7.2.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.

4., the preparation of photosynthetic bacterium liquid bacterial classification

Take out culture presevation pipe, draw flat board recover by photosynthetic bacterium solid culture medium, in illumination box, 35 degree are cultivated 72 hours.Intensity of illumination: 1000lex-6000lex.Under flat board, in 50 milliliters of photosynthetic bacterium liquid substratum of the single colony inoculation of picking, in illumination box, 35 degree anaerobism are cultivated 120 hours.Seed adopts 10% inoculum size, is seeded to the large triangular flask of 5L, and in illumination box, 35 degree anaerobism are cultivated 72-96 hour, detect its bacterial concentration, and bacterial content exceedes 6,000,000,000/milliliter, can be used as the inoculation of solution of photosynthetic bacteria body bacterial classification seed.Wherein said photosynthetic bacterium solid medium: potassium primary phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, sodium-acetate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium-chlor 1g, agar 20g, pH7.5, water 1000mL.Wherein said photosynthetic bacterium liquid substratum: potassium primary phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, sodium-acetate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium-chlor 1g, pH7.5, water 1000mL.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.

5., the preparation of Jingyang streptomycete liquid spawn

Take out culture presevation pipe, draw flat board recover with Jingyang streptomycete solid medium, in incubator, 30 degree are cultivated 72 hours.On flat board, in 50 milliliters of Jingyang streptomycete liquid nutrient mediums of the single colony inoculation of picking, in incubator, 30 degree concussions are cultivated 72 hours.Seed adopts 10% inoculum size, is seeded in the large triangular flask of 5L, and 72-96 hour is cultivated in 30 degree concussions, detects its bacterial concentration, and bacterial content exceedes 100,000,000/milliliter, can be used as Jingyang streptomycete liquid spawn seed inoculation.Wherein said Jingyang streptomycete solid medium: saltpetre 1g, Zulkovsky starch 20g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium-chlor 0.5g, ferrous sulfate 0.01g, agar 20g, water 1000ml, pH 7.2-7.4.Wherein said Jingyang streptomycete seed substratum: saltpetre 1g, Zulkovsky starch 20g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium-chlor 0.5g, ferrous sulfate 0.01g, water 1000ml, pH 7.2-7.4.The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.

Adopt the beneficial effect of technique scheme: this complex microorganism liquid bacterial fertilizer contains photosynthetic bacterium, photosynthetic bacterium can promote soil material to transform, and improves Soil structure, increases soil fertility, and promotes plant growth.Photosynthetic bacterium is mostly had a nitrogen fixing capacity, can improve soil nitrogen level, can effectively improve some organic composition, sulfide, ammonia-state nitrogen in soil, and promote the conversion of noxious pollutant (as agricultural chemicals, chemical fertilizer) by its Metabolic activity.Can promote beneficial microorganism propagation, make jointly to participate in the material cycle of soil ecology simultaneously.In addition, the abundant physiologically active substance that photosynthetic bacterium produces can directly be absorbed by plant as proline(Pro), uridylic, cytosine(Cyt), VITAMIN, ubiquinone, carotenoid etc., contribute to improve crop alimentary, the activity of activated plant cell, promote root system development, improve photosynthesis and reproductive growth ability.In Fermentation by Photosynthetic Bacteria liquid, contain several physiological active substances, as Chlorophyll A, B, carotenoid, multivitamin (especially vitamin B group), various plants hormone (as IAA, GA3, ABA, ethene, phytokinin), nucleic acid, Whitfield's ointment, multiple amino acids and single-cell protein etc.These physiologically active substances have or promote plant root growth or promote plant leaf growth or promote the accumulation of some specific component in fruit or increase crop yield, thereby improve quality of agricultural product.Photosynthetic bacterium can strengthen crop disease-resistant diseases prevention ability.Photosynthetic bacterium contains antibacterium, antiviral material, the virulence of these substance passivation pathogenic agent and inhibition pathogenic growth.The activity of photosynthetic bacterium simultaneously can promote the beneficial microorganism propagation such as actinomycetes, suppresses the pernicious bacteria growths such as filamentous fungus, thereby effectively suppresses some generation of planting disease and spread.Because photosynthetic bacterium has defending and fighting against diseases effect, can extend the fruit shelf lives.Photosynthetic bacterium can be improved plant chlorophyll content, promote the growth of the microorganism species such as vinelandii in soil, root nodule bacterium, actinomycetes, in tobacco, radish, sweet potato, Soybean and Other Crops, spray after photosynthetic bacterium, all can improve vinelandii in soil, root nodule bacterium, actinomycetes and bacterial number, filamentous fungus reduces, and plays the effect of soil remediation.Photosynthetic bacterium also has and alleviates murder by poisoning and the deteriorating pesticide residue of agricultural chemicals to farm crop.

This complex microorganism liquid bacterial fertilizer contains yeast, yeast utilizes the sugar in molasses, synthetic amino acid, carbohydrate and other organic substances of secretory product, photosynthetic bacteria that plant root produces to produce fermenting power, synthetic root growth and the fissional active substance of promoting.Yeast can promote other effective microbes (as milk-acid bacteria, actinomycetes) to breed needed matrix (food) provides important subsistence support.

This complex microorganism liquid bacterial fertilizer contains milk-acid bacteria.The carbohydrate that it produces by picked-up photosynthetic bacterium, yeast forms lactic acid.Lactic acid has very strong sterilizing ability, can effectively suppress the movable and organic sharply corrupt decomposition of harmful microbe.Milk-acid bacteria can be decomposed not labile xylogen and Mierocrystalline cellulose under normal conditions, and organic matter fermentation is decomposed.Milk-acid bacteria can also suppress the pathogenic bacterium propagation that continuous cropping obstacle produces.Pathogenic bacterium are active, and harmful nematode can sharply increase, and plant will be weak, and milk-acid bacteria has suppressed pathogenic bacterium, and harmful nematode just can fade away.

This complex microorganism liquid bacterial fertilizer contains Jingyang streptomycete, Jingyang streptomycete is a kind of actinomycetes, the Metabolic activity of Jingyang streptomycete can promote organic substance decomposing, make nutrient effectuation, can discharge antimicrobial substance simultaneously, thereby effectively prevent various crop disease and pest, and can produce the phytokinin of stimulating plant growth, it can also promote the formation of soil agreegate, have the formation that promotes soil ulmin and the ability that produces polysaccharide, the mixed clean bacterium effect of Jingyang streptomycete and photosynthetic bacterium is more much bigger than the lethality of Jingyang streptomycete individual combat.Jingyang streptomycete also can promote vinelandii and VA mycodomatium bacterium propagation.

This complex microorganism liquid bacterial fertilizer contains rich sporiferous subtilis, genus bacillus is the important component part of Plant diseases Biocontrol microorganism, there is significant Biocontrol Potential, can produce the contrary gemma of heat-resistant, be beneficial to production, the formulation of biocontrol fungicide and in environment, survive, surely grow and breeding.At stability, and the aspect such as the consistency of chemical pesticide, be obviously better than non-genus bacillus and fungi biocontrol fungicide.It can prevent and treat the plurality of plant diseases such as cotton wilt, tobacco mosaic virus (TMV), take-all, rice blast, leaf blight, cucumber at seedling stage samping off, potato scab, vegetable sprout term dry thread Pyrenomycetes, spinach blight, wheat scab, the red carninomatosis of apple, root rotof flax, ring rot of apple.

This complex microorganism liquid bacterial fertilizer contains a large amount of Chinese prickly ashes, capsicum, and Ginkgo Leaf, China tree, kuh-seng, the vegetable materials such as garlic, can drive away or kill white plant hopper, aphid, nematode, looper, oriental tobacco budworm, the insects such as tangerine a red-spotted lizard, leek ground maggot, eucleid caterpillar.In various probiotics fermention processes, destroyed its cell walls and can make effective ingredient discharge in a large number, greatly strengthened its deinsectization in probiotics fermention process, the ability of kill harmful bacterium, has reduced the utilization of its vegetable material.

This complex microorganism liquid bacterial fertilizer contains abundant calcium, magnesium, trace element, soybean protein, and under multiple bacterium co-fermentation, produce a large amount of organic acids, amino acid and many active substances, organic acid in these elements and fermented liq, amino acid are complex status or negative and positive particle equilibrium state, the namely lytic activity state in being easily absorbed by plants, play multi-efficiencies such as replenishing the calcium, mend magnesium, fertilising, rehabilitating soil, volume increase, this is also the advantage of microbial liquid fertilizer, is that other chemical fertilizer and trace element cannot replace.

Above-mentioned component coordinates and has that probiotics viable bacteria content is high, vegetable material consumption is few, activity is high, stability is high, action effect is fast; kill harmful bacterium, controlling plant diseases worm, replenish the calcium, mend the multi-efficiency of magnesium, fertilising; and undergo microbial fermentation its effect is multiplied; many things once complete; greatly reduce labour intensity; raw material is mainly probiotic bacterium, frequently seen plants fruit and leaf and sugar refinery waste liquid; cost is low; environment is not only formed to destruction, and also have very large provide protection.This technology tallies with the national condition and agricultural development direction, on agricultural planting, has broad application prospects.

The field effect (being called for short bacterial manure below) of preventing and treating the complex microorganism liquid bacterial fertilizer of plant pest below in conjunction with test explanation the present invention.

One, the effect of complex microorganism liquid bacterial fertilizer on tobacco

1. test site: Long Zhu village, beachhead town, Longhui County, Hunan Province Cotton Varieties by Small Farming Households ground

2. test method: flue-cured tobacco cultivars: middle cigarette 99-10-1, when flue-cured tobacco transplanting seedling, uses 500 times of dilution liquid irrigating roots of bacterial manure once; After slow seedling, within 20 days, spray for the first time with 250 times of liquid of bacterial manure, then sprayed for the second time every 20 days continuously and (altogether every mu with 4 kilograms, bacterial manure) for the third time.Check plot in spraying with 250 times of liquid of bacterial manure, with the clear water sprinkling of same consumption at every turn;

3. test-results:

Use after complex microorganism liquid bacterial fertilizer, plant height, stem girth, pitch, leaves number, maximum leaf area are all greater than the contrast that sprays clear water, and explanation can effectively improve the economical character of cigarette strain, promote the healthy and strong growth of cigarette strain.Cigarette strain sickness rate and the occurring degree used significantly reduce, and can effectively improve the disease resistance of cigarette strain, especially mosaic disease are had to good control effect.As can be seen from Table 1, according to the Major Diseases of field investigation, a situation arises, and sickness rate and the disease index of balck shank obviously reduce; Bacterial wilt does not find only there is a small amount of generation in contrast on treatment zone; Very low for the treatment of zone mosaic disease sickness rate and disease index, is only 6.3 and 1.2, reaches and controls extremely significantly effect;

Table 1 complex microorganism liquid bacterial fertilizer is to tobacco disease prevention effect

Table 2 complex microorganism liquid bacterial fertilizer is to tobacco production output value the effect of increasing income

Process	Output (kg/acre)	The output value (unit/mu)	Average price (unit/kilogram)
				Check plot	198.2	2505.2	12.64
Treatment zone	247.4	3527.9	14.26

Use tobacco premature ripening after complex microorganism liquid bacterial fertilizer, improve quality, improve to a great extent output, average price, the output value, the upper medium grade cigarette ratio of tobacco leaf.Can analyze and draw from table 2, experiment and demonstration district yield of tobacco, the output value, average price exceed 49.2 kgs/acre, 1022.7 yuan/mu, 1.62 yuan/kilogram than control zone respectively.370 yuan of the costs (wherein artificial 310 yuan) that deduct increase, can have a net increase of 652.7 yuan/mu.Using this bacterial fertilizer products can make tobacco leaf per mu yield increase by 24.8%, tobacco leaf per mu yield value net increase 40.8%.

Two, the test effect of complex microorganism liquid bacterial fertilizer on paddy rice

1. test site: peasant household of Long Zhu village, beachhead town, Longhui County, Hunan Province good farmland

2. test method:

(1). with 100 times of complex microorganism liquid bacterial fertilizer diluent seed soaking 6-8 hour;

(2). spray once with 250 times of diluents of complex microorganism liquid bacterial fertilizer of 1000 milliliters/mu respectively in seeding stage, tillering phase, boot stage;

3. test-results:

(1). the impact effect to paddy rice percentage of germination is best. and can promote seed germination, take root, improve germinating energy and percentage of germination, germinating energy amplification is 7.9～10.75%, and percentage of germination amplification is 8.8～9.5%, germinate fast and neat, well developed root system, thick white root is many, and adult seedling rate is high;

(2). per unit area yield is grown, improved to raising rice seedlings and grown in field all positive effect, and especially, to improving seedling quality, height survives seedling, and raising thousand seed weight etc. has remarkable effect.Plot experiment is along with using difference in period, and every mu can be increased production paddy 52-96 kilogram, and amount of increase in production is 8～12%, additional income 104-200 unit, and 60 yuan of deduction costs, mu has a net increase of income 44-140 unit;

(3). improve the drought resisting of paddy rice, the resistance such as disease-resistant, resistant to lodging, to various soil-borne diseases and bacterial disease, especially the diseases such as rice blast, banded sclerotial blight, damping-off are had to stronger inhibition and an effect of prevention.

Three, the effect of complex microorganism liquid bacterial fertilizer on apple

1. test site: Qiansuo Town, Suizhong County, Liaoning Province

2. test method: test is divided into: check plot is exactly the region that does not use bacterial manure.Test site, the region of use bacterial manure.The operation of two other fertilizer of Zoned application and other farming is identical, rushes application of water, and contrast also uses identical water.Test site: in spring, 500 times of punchings of bacterial manure dilution are executed or spreaded manuer in holes.In apple flowering period and fruit expanding period with once (mu is used 5 kilograms altogether) of the each spraying of 250 times of diluents of bacterial manure;

3. test-results

Use after foliage-spray in flowering period, investigation finds that apple fruit setting rate obviously improves, and compares with contrast, and test site percentage of fertile fruit improves 8-10 %; After expanding stage uses, compare with contrast, test site apple deformity number obviously reduces, when results, use the one-level fruit of bacterial manure and improved 18% than check plot, and fruit size is more even, output obviously improves, and yield increased district, test site output has improved 20%; The more important thing is that fruit color is even, surface is round and smooth, and brightness increases, and pol increases 2-3 degree Beaume, and shape delicious food is better.Check plot has apple to occur the symptom of bitter pit, elephant skin, water core, casting skin deliquescing, and the calcium that test site bacterial manure contains the needed various chelating states of fruit tree, magnesium and trace element are easy to fruit tree absorb, and do not occur these symptoms.

Four, complex microorganism liquid bacterial fertilizer test effect on strawberry

1. test site: plantation family, Tao Hong town, Longhui County, Hunan Province

2. test method: at setting date, seedling is planted, use 500 times of dilution liquid irrigating roots of bacterial manure 1 time,

At strawberry growing period, foliage-spray 1:250 times bacterial manure, 10d(days) 1 time, connect and spray 3 times;

3. test-results:

Found that, Blooming result, fruit is large and full, number of results is many, heterocarp is few, mouthfeel is good, painted vivid, the single plant yield average out to 126.96g of strawberry (Feng Xiang), contrast (not spraying bacterial manure) improves 28.07%, and use the soluble solid content of strawberry fruit after bacterial manure and reach 10.12%, contrast is relatively high by 13.8%, simultaneously, use after bacterial manure, acid content reduces, Vitamin C content improves, the local flavor of fruit and quality be improved significantly, and resistance to storage, than the many storages of the strawberry that does not use bacterial manure more than 10 days, strawberry root is stout and strong, Powdery Mildew and the root rot of strawberry have not had yet.Use the plot of bacterial manure to improve 28% than the output of check plot, pol has increased 2-3 degree than check plot.

Five, the application test of complex microorganism liquid bacterial fertilizer on muskmelon

1, test period: in February, 2011-June

2, test site: plantation family, Cang County, Hebei province booth

3, test method: use 100 times of vacuole kinds of bacterial manure 2 hours; Emerge and spray for the first time with 250 times of liquid of bacterial manure for latter 10 days, then sprayed for the second time to the every 20 days continuously four times (altogether every mu with 4 kilograms, bacterial manure);

4, test-results:

(1). output increases, and quality improves.Reach 4689 kilograms by the muskmelon per mu yield of bacterial manure, than contrast volume increase 18.2%, single melon weighs 1.46 kilograms, increases 0.214 kilogram than contrast; Refractive power pol 16.8, improves 1.62 than contrast;

(2). disease is light, and disease resistance is strong.According to investigation on June 25, the disease index 4.5 of oidium, has reduced by 63.4% than 12.3 of check plot.The disease index 6.5 of Powdery Mildew, has reduced by 34% than 10.3 of check plot.There are a large amount of melon aphids at back, check plot later stage blade face, but test site there is no;

(3). less investment, high efficiency, is used every mu, bacterial manure to increase input 60 yuan, and recruitment cost increases by 100 yuan, always increases by 160 yuan, can increase by 500 yuan of left and right of net return, input-output ratio 1:3.

Six, complex microorganism liquid bacterial fertilizer is at the effect of tomato

1. test period: in June, 2011-September

2. test site: plantation family, Du Lin township, Cang County, Hebei province

3. test method: before transplanting, seedling is stained with to 30 points of kinds of root with 200 times of bacterial manure diluents, after 10 days, uses 500 times of dilution liquid irrigating roots of bacterial manure 1 time after field planting, at tomato growing period, foliage-spray 1:250 times bacterial manure, 20 days 1 time, connects and spray 3 times;

4. test-results: found that, Blooming result, fruit is large and full, and number of results is many, and heterocarp is few, mouthfeel is good, painted vivid, the average plant height of tomato contrasts (not spraying bacterial manure) and improves 11%, and yield increased increases 23%, compare photograph, can effectively prevent the tomato fruit blossom-end rot being caused by calcium deficiency.

Seven, complex microorganism liquid bacterial fertilizer is to the sick test of Chinese cabbage Perishing prevention

1, test period: 9-2010 October in 2010, spray before at 9 o'clock in the morning or after at 4 o'clock in afternoon;

2, test site: Cangzhou City peasant household vegetable plot;

3, test method: bacterial manure 1:250 is doubly diluted fully, spray leaf positive and negative.Opening quotation phase, bag heart stage spray 2-3 time, wherein wrap heart stage spray 1-2 time.3000～4000 times of liquid foliage-sprays of agricultural streptomycin of 72% for check plot;

4, test effect: spray investigation in latter 2 days, fallen ill stop morbidity; Investigation in the 4th day, plant restore normal growth.November is while results, than check plot volume increase 10.8%.

Eight, complex microorganism liquid bacterial fertilizer is to Radix Gossypii maize ear rot control test

1, test period: in August, 2010-2010 year September

2, test site: Cangzhou City peasant household cotton field, has the rotten pathogenetic region of Radix Gossypii in various degree every year;

3, test method: bacterial manure 1:500 doubly dilutes overcast and rainy rear filling root.After every two weeks, fill with once, bacterial manure usage quantity is each 1 liter/mu again;

4, test-results: check plot sickness rate reaches 20-40%, and executes the not generation disease of our bacterial manure.Reach 45% with check plot volume increase.

Nine, the control test of complex microorganism liquid bacterial fertilizer to radish aphid

1, test period: on February 13rd, 2011, at 4 o'clock in afternoon

2, test site: plantation vegetable plot, family, Longhui County, Hunan Province

3, test method: bacterial manure 1:250 doubly dilutes and sprays;

4, test-results: spray latter second day, green aphid is all dead.Black aphid has not had substantially yet, should have been driven away by the smell of bacterial manure.

Ten, the control of complex microorganism liquid bacterial fertilizer to ginger root knot nematode

1, test site: plantation vegetable plot, family, Longhui County, Hunan Province

2, test method: in ginger children's phase, bacterial manure 1:250 doubly dilutes and sprays once.In ginger vegetative period, bacterial manure 1:500 doubly dilutes rear filling root, and utilizes bacterial manure 1:250 doubly to dilute to spray once simultaneously;

3, test-results: the ginger of general planting, rot, root knot nematode are serious, disease-free with the ginger of bacterial manure.

Embodiment

Substantive distinguishing features of the present invention can be embodied from the following examples, but only conduct explanation of these embodiment, instead of limit the invention.

Embodiment 1

The raw material of preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 5 parts, China tree powder, 5 parts, kuh-seng powder, 5 parts of salt, 30 parts of mashed garlics, 50 parts, molasses, 5 parts of analysis for soybean powder, 10 parts, calcium sulfate, 10 parts, magnesium chloride, be greater than 10 parts of 6,000,000,000/milliliter photosynthetic bacteriums, be greater than 10 parts of 500,000,000/ml yeast bacterium, be greater than 10 parts of 10,000,000,000/milliliter plant lactobacillus, be greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, be greater than 10 parts of 6,000,000,000/milliliter subtilises, 800 parts, water.

The preparation method of the complex microorganism liquid bacterial fertilizer of this control plant pest comprises the steps:

(1), in fermentor tank, add successively: 50 parts, molasses, 5 parts of analysis for soybean powder, 10 parts, calcium sulfate, 10 parts, magnesium chloride, 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 5 parts, China tree powder, 5 parts, kuh-seng powder, 5 parts of salt, 800 parts, water, heated and boiled 30 minutes, is cooled to 40 degree;

(2) the cooling fluid of, (1) producing in step adds 10 parts of 30 parts of mashed garlics, 6,000,000,000/milliliter photosynthetic bacteriums, 10 parts of 500,000,000/ml yeast bacterium, 10 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 10 parts of 6,000,000,000/milliliter subtilises;

Embodiment 2

The raw material of preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 20 parts of zanthoxylum powders, 10 parts of red chilly powders, 10 parts of ginkgo leaf powders, 10 parts, China tree powder, 5 parts, kuh-seng powder, 5 parts of salt, 20 parts of mashed garlics, 50 parts, molasses, 5 parts of analysis for soybean powder, 10 parts, calcium sulfate, 10 parts, magnesium chloride, be greater than 15 parts of 6,000,000,000/milliliter photosynthetic bacteriums, be greater than 5 parts of 500,000,000/ml yeast bacterium, be greater than 5 parts of 10,000,000,000/milliliter plant lactobacillus, be greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, be greater than 10 parts of 6,000,000,000/milliliter subtilises, 800 parts, water.

(1), in fermentor tank, add successively: 50 parts, molasses, 5 parts of analysis for soybean powder, 10 parts, calcium sulfate, 10 parts, magnesium chloride, 20 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 10 parts, China tree powder, 5 parts, kuh-seng powder, 5 parts of salt, 810 parts, water, heated and boiled 30 minutes, is cooled to 40 degree;

(2) the cooling fluid of, (1) producing in step adds 15 parts of 20 parts of mashed garlics, 6,000,000,000/milliliter photosynthetic bacteriums, 5 parts of 500,000,000/ml yeast bacterium, 5 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 10 parts of 6,000,000,000/milliliter subtilises;

Embodiment 3

The raw material of preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 5 parts, China tree powder, 5 parts, kuh-seng powder, 5 parts of salt, 30 parts of mashed garlics, 50 parts, molasses, 5 parts of analysis for soybean powder, 10 parts, calcium sulfate, 10 parts, magnesium chloride, be greater than 15 parts of 6,000,000,000/milliliter photosynthetic bacteriums, be greater than 10 parts of 500,000,000/ml yeast bacterium, be greater than 10 parts of 10,000,000,000/milliliter plant lactobacillus, be greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, be greater than 15 parts of 6,000,000,000/milliliter subtilises, 800 parts, water.

(2) the cooling fluid of, (1) producing in step adds 15 parts of 30 parts of mashed garlics, 6,000,000,000/milliliter photosynthetic bacteriums, 10 parts of 500,000,000/ml yeast bacterium, 10 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 15 parts of 6,000,000,000/milliliter subtilises;

Embodiment 4

The raw material of preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 10 parts of ginkgo leaf powders, 10 parts, China tree powder, 5 parts, kuh-seng powder, 5 parts of salt, 20 parts of mashed garlics, 50 parts, molasses, 5 parts of analysis for soybean powder, 15 parts, calcium sulfate, 10 parts, magnesium chloride, be greater than 10 parts of 6,000,000,000/milliliter photosynthetic bacteriums, be greater than 10 parts of 500,000,000/ml yeast bacterium, be greater than 10 parts of 10,000,000,000/milliliter plant lactobacillus, be greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, be greater than 15 parts of 6,000,000,000/milliliter subtilises, 800 parts, water.

(1), in fermentor tank, add successively: 50 parts, molasses, 5 parts of analysis for soybean powder, 15 parts, calcium sulfate, 10 parts, magnesium chloride, 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 10 parts of ginkgo leaf powders, 10 parts, China tree powder, 5 parts, kuh-seng powder, 5 parts of salt, 800 parts, water, heated and boiled 30 minutes, is cooled to 40 degree;

(2) the cooling fluid of, (1) producing in step adds 10 parts of 20 parts of mashed garlics, 6,000,000,000/milliliter photosynthetic bacteriums, 10 parts of 500,000,000/ml yeast bacterium, 10 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 15 parts of 6,000,000,000/milliliter subtilises;

Claims

1. a preparation method who prevents and treats the complex microorganism liquid bacterial fertilizer of plant pest, is characterized in that:

The raw material of preparing this bacterial manure is by weight: zanthoxylum powder 10-20 part, red chilly powder 10-20 part, ginkgo leaf powder 5-15 part, China tree powder 5-15 part, kuh-seng powder 5-15 part, molasses 40-80 part, analysis for soybean powder 4-8 part, calcium sulfate 5-15 part, magnesium chloride 5-15 part, salt 5-10 part, mashed garlic 20-40 part, be greater than 6,000,000,000/milliliter photosynthetic bacterium bacterial classification 5-15 part, be greater than 500,000,000/ml yeast bacterium bacterial classification 5-15 part, be greater than 10,000,000,000/milliliter plant lactobacillus bacterial classification 5-15 part, be greater than 100,000,000/milliliter Jingyang streptomyces species 5-15 part, be greater than 6,000,000,000/milliliter Bacillus subtilis strain 5-15 part, 800 parts, water,

Preparation method comprises the steps:

(2) the cooling fluid of, (1) producing in step adds mashed garlic 20-40 part, is greater than 6,000,000,000/milliliter photosynthetic bacterium bacterial classification 5-15 part, is greater than 500,000,000/ml yeast bacterium bacterial classification 5-15 part, is greater than 10,000,000,000/milliliter lactic acid bacteria culturers 5-15 part, is greater than 100,000,000/milliliter Jingyang streptomyces species 5-15 part, is greater than 6,000,000,000/milliliter Bacillus subtilis strain 5-15 part;

, emit four layers of filtered through gauze for bacterium liquid, packaging final prod,

Bacillus subtilis strain adopts following methods preparation:

The preparation of subtilis liquid spawn: take out culture presevation pipe, draw flat board recover with LB solid medium, 37 degree are cultivated 48 hours, under flat board, in 50 milliliters of LB substratum of the single colony inoculation of picking, in incubator, 37 degree concussions are cultivated 24 hours; Seed adopts 5% inoculum size, is seeded in the large triangular flask of 5L, and 20-28 hour is cultivated in 37 degree concussions, detects its bacterial concentration, and gemma content exceedes 6,000,000,000/milliliter, can be used as subtilis liquid-spawn inoculation; LB substratum: peptone 5g, extractum carnis 3g, sodium-chlor 5g, water 1000mL, pH7.2, adds agar 2% in solid medium; Subtilis liquid spawn culture medium: glucose 10g/L, Semen Maydis powder 8 g/L, bean cake powder 25 g/L, magnesium sulfate 0.5 g/L, manganous sulfate 0.5 g/L, SODIUM PHOSPHATE, MONOBASIC 0.5 g/L, Sodium phosphate dibasic 2.3 g/L, pH 7.2; The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes;

Milk-acid bacteria liquid bacterial classification adopts following methods preparation:

Take out culture presevation pipe, drawing flat board with MRS solid medium recovers, 37 degree anaerobism are cultivated 48 hours, under flat board in 50 milliliters of MRS substratum of the single colony inoculation of picking, in incubator, 37 degree anaerobism are cultivated 24 hours, milk-acid bacteria seed adopts 5% inoculum size, be seeded in the large triangular flask of 10L, 37 degree anaerobism are cultivated 20-28 hour, detect its bacterial concentration, bacterial content exceedes 10,000,000,000/milliliter, can be used as the inoculation of lactobacillus suspension body bacterial classification, MRS substratum: peptone 10g/L, yeast extract 5 g/L, extractum carnis 10 g/L, glucose 20 g/L, sodium-acetate 5 g/L, dibasic ammonium citrate 2 g/L, tween-80 1 ml/L, magnesium sulfate 0.6 g/L, manganous sulfate 0.05 g/L, dipotassium hydrogen phosphate 2 g/L, agar 20 g/L, calcium carbonate 20 g/L, in solid medium, add agar 20 g/L, milk-acid bacteria seed substratum: glucose 50 g/L, peptone 15 g/L, extractum carnis 7.5 g/L, sodium-chlor 1 g/L, manganous sulfate 0.01 g/L, magnesium sulfate 0. 2 g/L, sodium-acetate 0. 5 g/L, calcium carbonate 50 g/L, pH6.0, the condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes,

Yeast liquid bacterial classification adopts following methods preparation:

Take out culture presevation pipe, drawing flat board with PDA solid medium recovers, 30 degree are cultivated 48 hours, under flat board in 50 milliliters of PDA substratum of the single colony inoculation of picking, in incubator, 30 degree concussions are cultivated 24 hours, yeast seed adopts 5% inoculum size, be seeded in the large triangular flask of 5L, 20-28 hour is cultivated in 30 degree concussions, detect its bacterial concentration, bacterial content exceedes 500,000,000/milliliter, can be used as the inoculation of yeast liquid body bacterial classification, PDA substratum: potato 200g, sucrose 20g, water 1000mL, in solid medium, add agar 20 g/L, yeast seed substratum: glucose 10 g/L, yeast extract paste 3 g/L, peptone 5 g/L, ammonium sulfate 5 g/L, magnesium sulfate 0.5 g/L, the condition of+each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes,

Photosynthetic bacterium liquid bacterial classification adopts following methods preparation:

Take out culture presevation pipe, drawing flat board by photosynthetic bacterium solid culture medium recovers, in illumination box, 35 degree are cultivated 72 hours, intensity of illumination: 1000lex-6000lex, under flat board in 50 milliliters of photosynthetic bacterium liquid substratum of the single colony inoculation of picking, in illumination box, 35 degree anaerobism are cultivated 120 hours, photosynthetic bacterium seed adopts 10% inoculum size, be seeded to the large triangular flask of 5L, in illumination box, 35 degree anaerobism are cultivated 72-96 hour, detect its bacterial concentration, bacterial content exceedes 6,000,000,000/milliliter, can be used as the inoculation of solution of photosynthetic bacteria body bacterial classification seed, photosynthetic bacterium solid medium: potassium primary phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, sodium-acetate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium-chlor 1g, agar 20g, pH7.5, water 1000mL, photosynthetic bacterium liquid substratum: potassium primary phosphate 1g, calcium chloride 0.1g, sodium carbonate 3g, sodium-acetate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium-chlor 1g, pH7.5, water 1000mL, the condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes,

Jingyang streptomyces species adopts following methods preparation:

The preparation of Jingyang streptomycete liquid spawn: take out culture presevation pipe, draw flat board recover with Jingyang streptomycete solid medium, 30 degree are cultivated 72 hours in incubator; On flat board, in 50 milliliters of Jingyang streptomycete liquid nutrient mediums of the single colony inoculation of picking, in incubator, 30 degree concussions are cultivated 72 hours; Seed adopts 10% inoculum size, is seeded in the large triangular flask of 5L, and 72-96 hour is cultivated in 30 degree concussions, detect its bacterial concentration, bacterial content exceedes 100,000,000/milliliter, can be used as Jingyang streptomycete liquid spawn seed inoculation, Jingyang streptomycete solid medium: saltpetre 1g, Zulkovsky starch 20g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium-chlor 0.5g, ferrous sulfate 0.01g, agar 20g, water 1000ml, pH 7.2-7.4; Jingyang streptomycete seed substratum: saltpetre 1g, Zulkovsky starch 20g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium-chlor 0.5g, ferrous sulfate 0.01g, water 1000ml, pH 7.2-7.4; The condition of each medium sterilization is: 0.10-0.15MPa, 121 DEG C of sterilizings 30 minutes.

2. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1, is characterized in that, mashed garlic be by cooling boiling water with etc. after the garlic of weight mixes, pulling an oar makes.

3. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1, it is characterized in that, zanthoxylum powder, red chilly powder, ginkgo leaf powder, China tree powder, kuh-seng powder are that the Chinese prickly ash of buying from the market, capsicum, Ginkgo Leaf, China tree, kuh-seng were pulverized to 60 order-100 order gained.

4. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1, is characterized in that, photosynthetic bacterium bacterial classification is Rhodopseudomonas palustris, the one in Rhodopseudomonas spheroides.

5. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1, is characterized in that, barms is yeast saccharomyces cerevisiae, Candida utilis, one or more in candida tropicalis.

6. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1, is characterized in that, lactobacillus inoculation is plant lactobacillus, Lactobacterium acidophilum, one or more in lactobacterium casei.