CN1060917C - Production method for biological multi-bacterial fermented fodder made from stalks - Google Patents
Production method for biological multi-bacterial fermented fodder made from stalks Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The present invention relates to a method for producing biological multi-bacterial fermented fodder of crop stalks. Crop stalks are processed and converted to mycoprotein fodder by physics, chemistry and biology, particularly a coordinated fermentation method of multiple strains. The present invention has the advantages of high product yield rate, high crude protein content, large reduction of crude fiber contents, simple technique and low cost. The present invention is suitable for being popularized in a nationwide range.
Description
The present invention relates to a kind of production method of biological multi-bacterial fermented fodder made from stalks, belong to a kind of use physics, chemistry, biological integrated approach, in the agricultural crop straw culture material, insert multiple bacterial classification and carry out the cooperative fermentation processing, finally produce the processing method of bacterial classification protein fodder.
Have many pieces of patent documents to disclose all methods of utilizing the crop straws for producing feed in the prior art, wherein immediate documents is:
90105689.8 number Chinese patent discloses the method for utilizing straw to produce the bacterial classification protein fodder, it utilizes industrial residue, for example citric acid waste; the sugar slag; fecula directly carries out submerged fermentation with edible mushrooms and makes liquid spawn, this bacterial classification is received carried out solid fermentation in the comminuted plants stalk again; its shortcoming is: the one, and inoculum size is big; time-consuming taking a lot of work increased manufacturing cost, and the 2nd, fermentation period is long; increased the equipment input, be difficult to accomplish scale production.
Chinese patent 90107802.6 discloses the method for utilizing straw to produce animal feeding-stuff containing somatic protein; it utilizes edible mushrooms to make mycelium; inoculate the culture material conversion of fermenting, its shortcoming is: it is long to make mycelia kind and material fermentation time, can't accomplish scale production.
No. 87104802 Chinese patents then disclose and have utilized thick fecula inoculation geotrichum candidum, aspergillus niger strain carries out the method that solid fermentation prepares animal feeding-stuff containing somatic protein, owing to select for use thick fecula as culture material, then there is the raw material sources deficiency, it applies the restriction that can be subjected to resource, the manufacturability that does not possess the nationwide promotion and implementation, in addition, this method is diverted from one use to another in straw, then because the shortage of auxiliary bacteria and the problem on the main bacteria seed compatibility, feasible fermentation to stalk does not reach the standard of producing feed, and promptly this production method suitability is relatively poor.
Add up according to pertinent data, the whole world has 5,000 hundred million tons of cellulose resource every year approximately, China has 5,000,000,000 tons approximately, wherein straw (wheat straw, straw, corn stalk etc.) just reaches 500,000,000 tons, it is minority that these stalks can be used for feeding, major part acts as a fuel and fertilizer, even also adopt traditional direct feeding method as the small portion of feed, digestive utilization ratio is low more; If it is carried out certain fermentative processing, can improve the digestibility of animal greatly, because stalk resource is very abundant, regeneration is carried out fermentative processing to stalk and can be saved grain every year, alleviates China's grain pressure in short supply;
The production method that the objective of the invention is to overcome the deficiencies in the prior art and a kind of biological multi-bacterial fermented fodder made from stalks is provided, this method is used physics, chemistry, biological Synergistic method carries out fermentative processing to agricultural crop straw, applies multiple bacterial classification, acts synergistically, crop material is transformed, making it crude protein content increases, and crude fiber content reduces greatly, and comprehensive nutrient is good;
The production method of biological multi-bacterial fermented fodder made from stalks of the present invention comprises following two steps:
1) preparation of raw material comprises the preparation of culture material and the preparation of bacterial classification, separates purifying;
2) bacterial classification is inserted in the culture material, ferment;
Wherein the preparation of culture material is expressed as with weight percent:
Crop material (wheat, corn, straw) was pulverized the 5-25 order, straw powder with 80-95% adds wheat bran 0-15%, Semen Maydis powder 0-12%, urea 0-1%, calcium superphosphate 0-0.8%, sodium-chlor 0-1%, bone meal 0-1%, dipotassium hydrogen phosphate 0-1%, ammonium chloride 0-0.3%, magnesium chloride 0-0.8% transfers pH value to 4.5-5.5 with phosphoric acid, normal-pressure sterilization half an hour or unsterilised, cool to 25-35 ℃;
The bacterial classification that inserts in the culture material is that 100% weight is that benchmark is counted with culture material:
Yeast geotrichum candidum AS2.498 seed liquor 6-14% and green lignin AS3.3032 solid seed 0.5-2% compatibility; Or
Fungi aspergillus niger AS3.350 solid seed 3-6% and yeast Candida utilis AS2.1180 seed liquor 15-25% compatibility; Or
Yeast geotrichum candidum AS2.361/AS2.498 seed liquor 6-13% and viride AS3.2928 solid seed 0.2-1.2% and mould AS3.287 solid seed 0.4-1.0% compatibility;
More than the bacterial classification of various compatibilities add liquid photosynthetic bacterium seed liquor 0-5%, high-activity yeast bacterium 0-0.02%, saccharifying enzyme 0-0.8%, milk-acid bacteria 0-0.5% again;
According to quantity bacterial classification is joined in the culture material, mix, insert fermentor tank or fermentation machine or fermentation vat, cultivated 40-80 hour down at temperature 25-35 ℃;
After fermenting, can store with the grain preparing complete feed such as corn or the oven dry of granulating.
In the present invention, at first adding nitrogen, phosphorus, potassium growth-promoting agent in straw handles, add multiple synergistic bacterium then, liquid geotrichum candidum wherein act as raising albumen, this mould decomposition fiber that act as, the liquid photosynthetic bacterium act as the comprehensive nutrient composition that improves feed, acting as of saccharifying enzyme helps catalysis, decomposition of cellulose, saccharomycetic acting as improved albumen, amino acid, milk-acid bacteria act as the palatability of improving fermentation condition and feed.
The production method of biological multi-bacterial fermented fodder made from stalks of the present invention has the following advantages and positively effect:
1. the agricultural crop straw consumption is big, almost reaches 95%, and raw material sources are extensive, and the stalk consumption has only 30-50% in the method for prior art, and feed making cost of the present invention is low;
2. employing multi-strain fermentation, fibre content reduces, and protein content increases, and comprehensive nutrient is good, and culture efficiency is better than existing fermented feed, and also better than full grain fodder, meat ratio reaches 1: 3.2.
3. adopt physics, chemistry, biological integrated conduct method, the product yield is up to 90%, and crude protein content is more than 10%, and robust fibre is reduced to about 20% by original about 40%.
4. be rich in the continuation breeding in the animal digestion absorption system of biologically active substance such as Coenzyme Q10 99.0 and the panimmunity short long factor, especially biologically active substance, thereby the immunity system that has activated body has been strengthened the stress reaction of animal body.
5. with short production cycle, be generally in 48 hours.
6. existing fermented feed mainly is to be used for ox, sheep (ruminate, herbivorous animal) feed, the present invention can be used for chicken, pig, fish meal, after particularly making granulated feed, because the ratio height that farm crop straw organism feed accounts in the feed, volume is big, proportion is little, and the time that suspends in water is about 1 hour, is particularly suitable for using as fish meal.
7. the feed that method of the present invention makes contains more rich amino acid and trace element, be rich in little element, folic acid, vitamin H, antiviral substance and the somatomedin of giving birth to of abundant B family, this feed is fed through livestock and poultry can strengthen resistance against diseases, promote growth of animals or poultry, be the good inferior material of fowl poultry kind grain, its economic benefit and social benefit are quite remarkable.
For introducing advantage of the present invention and effect in detail, quote some charts below and be elaborated:
Table 1 is for utilizing the nutrition table of the product that method of the present invention makes;
The effect report that table 2 is fed laying hen for the farm crop straw organism fermented feed;
Table 3 is farm crop straw organism fermented feed growing and fattening pigs trial reports;
Table 4 blendes together the complete feed proportioning of chicken feed for the farm crop straw organism fermented feed;
Table 5 blendes together the complete feed proportioning of pig feed for the farm crop straw organism fermented feed.
The present invention utilizes that microbial liquid and solid geotrichum candidum, wood are mould, liquid photosynthetic bacterium bacterial classification and saccharifying enzyme, yeast, milk-acid bacteria cooperative fermentation are produced the farm crop straw organism granulated feed.The present invention can handle a large amount of agricultural crop straws, weeds and agricultural byproducts waste thereof by the pulverizing and the homogenizing of physics, add growth-promoting agents such as nitrogen, phosphorus, potassium then and carry out chemical treatment, utilize above-mentioned multiple bacterial classification to carry out biological fermentation at last, make it to be converted into animal feeding-stuff containing somatic protein.Technology of the present invention is simple, for the agricultural crop straw raw material, can carry out unsterilised solid fermentation, the solid fermentation of also can sterilizing is for the former, the investment of required equipment is less, but the quantity of inoculation bacterial classification is higher, and for the latter, the required equipment investment is big, corresponding, the consumption of the bacterial classification of its inoculation is smaller;
Agricultural crop straw transforms through above-mentioned physics, chemistry, bioprocess and makes albumen bring up to 9-16% by 3-4%, robust fibre is reduced to about 20% by former about 40%, contain more rich amino acid and trace element, be rich in little element, folic acid, vitamin H, antiviral substance and the somatomedin of giving birth to of abundant B family, this feed is through the livestock and poultry enhancing resistance against diseases of feeding, promoting growth of animals or poultry, is good fowl poultry kind grain fodder, and its economic benefit and social benefit are quite remarkable.
Agricultural crop straw improves through its nutritive ingredient of microbial transformation, feeds through the livestock and poultry examination to replace grain 40-70%, and every nutritive index meets national forage standard, through experiment, every day, every pig fed 2.2 jin of biological granulated forages, and 0.75 jin of day weight gain is fed into its hair color of pig, meat is better.Present technique is made granulated feed from crop material physics, chemical treatment and biological fermentation, and its cost is 1.3 yuan/Kg.
The granulated feed that present technique is made is through feeding pig test, have save food, diseases prevention, advantages such as livestock and poultry animal fast gaining.
Used following bacterial classification among the present invention: wherein preceding 3 are adopted following detailed process production, saccharifying enzyme, and yeast and milk-acid bacteria all can be bought from market; Black aspergillus of liquid geotrichum candidum wherein and fungi and yeast Candida utilis act as raising albumen, the mould decomposition fiber that act as of wood, the comprehensive nutrient composition that act as the raising feed of liquid photosynthetic bacterium, acting as of saccharifying enzyme helps catalysis, decomposition of cellulose, saccharomycetic acting as improved albumen, amino acid, milk-acid bacteria act as the palatability of improving fermentation condition and feed.
The preparation that divides three steps to introduce former bacterial classification respectively below, the separation of bacterial classification and purifying, culture of strains
(1) former spawn culture
1. the preparation of the former bacterial classification in geotrichum candidum AS2.498 inclined-plane
A. culture medium raw material:
Sweet potato 200-400g, glucose 15-28g, agar powder 15-25g water 1000ml, ph 5.7-6.4
B. make slant medium and inoculation culture
Sweet potato peeling stripping and slicing, boiled 40 minutes, double gauze filters, and filtered juice adds water to 1000ml, adds agar powder, and the heating in water bath dissolving adds the glucose stirring and dissolving, and the test tube of packing into is with 1.1Kg/CM
2The inclined-plane is put in pressure 30 minutes sterilization, by the aseptic technique inoculation, puts 28-30 ℃ and cultivates 1-2 days, and the inclined-plane is covered with white velvet-like mycelium, can directly use, the also available mouth of sealing with wax, and it is standby to put shady and cool place's preservation.
2. the preparation of the former bacterial classification of viride AS3.3032 and mould AS3.287 inclined-plane
The A culture medium raw material:
Sweet potato 200-400g, glucose 15-28g, agar powder 15-25g, stalk fibre powder 15-25g, water 1000ml, ph4.7-5.4
B. make slant medium and inoculation culture:
Sweet potato peeling stripping and slicing, boiled 40 minutes, double gauze filters, and filtered juice adds water to 1000ml, adds agar powder, and the heating in water bath dissolving adds the glucose stirring and dissolving, and the test tube of packing into is with 1.1Kg/CM
2The inclined-plane is put in pressure sterilization in 30 minutes, by the aseptic technique inoculation, puts 28-30 ℃ and cultivates 5-7 days, and the inclined-plane grows evenly intensive green spores, promptly gets the former bacterial classification in inclined-plane.
3. the preparation of the former bacterial classification in fungi aspergillus niger AS3.350 inclined-plane
A. culture medium raw material:
Potato liquor 1000ml, glucose 15-28g,
Agar powder 15-25g, ph4.7-5.4
B. make slant medium and inoculation culture
Get potato liquor 1000ml, glucose 15-28g, agar powder 15-25g, the heating in water bath dissolving, transferring pH value with phosphoric acid is 4.7-5.4, the test tube of packing into is with 1.1Kg/CM
2The inclined-plane is put in pressure sterilization in 30 minutes, by the aseptic technique inoculation, puts under the 28-30 ℃ of temperature and cultivates 3-4 days, promptly gets the former bacterial classification in inclined-plane.
4. the preparation of the former bacterial classification in Candida utilis AS2.1180 inclined-plane
A. culture medium raw material:
The 10BX wort, natural pH value, 2% agar powder;
B. make slant medium and inoculation culture
Take by weighing wort according to quantity, agar powder, the heating in water bath dissolving, the test tube of packing into is with 1.1K/gCM
2The inclined-plane is put in pressure sterilization in 30 minutes, by the aseptic technique inoculation, puts under the 28-30 ℃ of temperature and cultivates 2-3 days, promptly gets the former bacterial classification in inclined-plane.
(2) separation of bacterial classification and purifying:
Material
1) gravy, agar, the glucose culture test tube wherein is equipped with and is used for a kind of of several bacterial classifications that stalk is fermented or two kinds;
2) substratum: test tube gravy, agar, glucose
3) experimental ware: a secondary sterile petri dish, water-soluble pot, spirit lamp, transfering loop, incubator, transfer room or darkroom;
2. operation steps:
1) bouillon media is melted;
2) will melt the test tube substratum dries;
3) left hand is held bacterial classification and substratum two test tubes, in aseptic processing room or darkroom, will ask for a ring mixture in substratum by aseptic technique;
4) micro oscillation substratum makes it and spares;
5) will pour in the culture dish with the substratum of even sample to be separated, shake up,, former bacteria culture fluid can be diluted in advance if cell count is excessive in the bacterium liquid;
6) uviolizing processing and pure strain are cultivated:
Open ultraviolet preheating 15 minutes earlier, make light wave stable, then culture dish is inserted darkroom or transfer room (sealing unglazed), shining 30 seconds to 2 minutes from fluorescent tube 25CM place, then culture dish is wrapped with black cloth and placed in the incubator, under 28-32 ℃ of temperature, cultivated 24 hours, select to cultivate to produce in the vigorous energetic bacterial strain access of the growth slant medium and plant;
(3) production culture of strains or preparation
1. geotrichum candidum AS2.361/AS2.498 culture of seed liquid
First kind of culture material and method:
Useless dish water, spent wash, bean curd water, agricultural crop straw water etc. all can, transferring pH value with phosphoric acid is 3.8-4.2, adds urea 0.3-0.7%, calcium superphosphate 0.2-0.4% boils dissolving, keeps 10-15 minute at 105 ℃, filter and remove residue adds yeast extract paste 0.1-0.2%, vitimin supplement again, adding ammoniacal liquor accent pH value is 4.7-5.3, be cooled to inoculation about 35 ℃, insert the interior 28-32 ℃ of fermentation of seeding tank or mash-back about 24 hours, be generally 20-28 hour;
Second kind of culture material and method:
Maltose 0.2-0.4%, an albumen 0.6-1.0%, pH value 4.8-5.2 boiled 30 minutes or sterilizes, and inserted after the cooling in seed liquor jar or the mash-back, and 28-32 ℃ of bottom fermentation is about 24 hours;
2. viride AS3.3032 and mould AS3.287 solid seed culture:
1) culture material:
Agricultural crop straw powder 70-90%, wheat bran 5-16%, corn 5-16%, each 0.3-0.6% of calcium superphosphate and ammonium sulfate transfers pH value to 3.8-4.1 with phosphoric acid, and amount of water webs when holding have water not ooze to be advisable;
2) cultivate:
After culture material stirs, with 1.1Kg/CM
2Pressure sterilization 30 minutes cools to about 35 ℃ inoculation and stirs, and puts in fermentation dish or the fermentation vat about 30 ℃ fermentations about 5 days, and the bed of material covers with, and green, white spore can be stored standby as bacterial classification or oven dry below 40 ℃;
3. fungi aspergillus niger AS3.350 solid seed culture:
1) culture material
The agricultural crop straw powder of 60-80%, the wheat bran of 20-40%, the Semen Maydis powder of 6-10%, urea 0.3-0.6%, sodium-chlor 0.3-0.6%, ammonium sulfate 0.3-0.7%, it is 4.7-5.4 that phosphoric acid is transferred pH value, and amount of water is that material-water ratio equals 1: (1.3-1.7), and 1.1Kg/CM
2Pressure sterilization 30 minutes;
2) cultivate
Culture material cools to about 45 ℃, connects slant strains, fully stirs, and inserts on fermentation container or the fermentation dish, cultivates 3-4 days under the 28-32 ℃ of temperature, can store standby as bacterial classification or oven dry below 40 ℃;
4. the preparation of Candida utilis AS2.1180 seed liquor:
1) culture material
Wine lees liquor filters, and adds 0.3-0.6% ammonium sulfate, 0.3-0.6% phosphoric acid, and the urea of 0.3-0.6%, pH value is controlled at 3.5-3.8.
2) cultivate
Above feed liquid is inserted shaking table, and inoculation culture cultivating about 18 hours under the 30-32 ℃ of condition, was generally 15-20 hour, can be as seed liquor;
5. the preparation of photosynthetic bacterium seed liquor:
Ammonium chloride 0.8-1.2g, potassium primary phosphate 0.3-0.5g, magnesium chloride 0.2g, sodium-chlor 1.7-2.2g, be dissolved in the 900ml tap water, add 5g sodium bicarbonate distilled water solution again, 0.1g yeast extract paste, ethanol 2ml, 4g wheat bran or Semen Maydis powder hydrolyzed solution, with the dissolving of material loading water thorough mixing, the photosynthetic bacterium of inoculation 10% is inserted in the transparent vial or Plastic Bottle, carry out artificial lighting or utilize solar light irradiation with 100 watts of bulbs, cultivating about 72 hours under 30 ℃ of temperature, for example 60-80 hour, can be as photosynthetic bacterium finished product or seed;
6. the resurrection of saccharifying enzyme and high-activity yeast:
Buy saccharifying enzyme and high-activity yeast from the market, take by weighing the stroke-physiological saline solution that adds about 5 times according to quantity, under 35 ℃ of temperature, make its dissolving, and kept temperature-activated 2 hours, can be added in the solid fermentation material then;
7. the dilution of milk-acid bacteria:
Milk-acid bacteria is bought from market, and with 3 times of stroke-physiological saline solution dilutions, can directly be added in the solid culture medium;
Specific embodiment:
1. geotrichum candidum AS2.498 and viride AS3.3032 compatibility seed, and add photosynthetic bacterium, milk-acid bacteria, high-activity yeast work in coordination with biosolids and is fermented:
1) the solid fermentation material formula is represented with weight percent:
95% agricultural crop straw powder, 2% wheat bran or Semen Maydis powder, 0.5% urea, 0.5% calcium superphosphate, 0.5% bone meal, 0.5% sodium-chlor, with phosphoric acid regulating ph value about 5, normal-pressure sterilization 30 minutes, cool to 45 ℃ after inoculation;
2) bacterial classification access amount is that 100% weight is benchmark in culture material:
Geotrichum candidum AS2.498 seed liquor 10%, viride AS3.3032 solid seed 1%, photosynthetic bacterium 1%, milk-acid bacteria 0.01%, high-activity yeast 0.01%;
3) inoculation and fermentation:
Bacterial classification is inserted in the culture material, stirs, insert in fermentation machine or the fermentation vat, under 28-32 ℃ of temperature, cultivated 48-72 hour, the bed of material cover with white hypha send fragrance can preparing complete feed or make the particle oven dry and store;
2. fungi aspergillus niger AS3.350 and yeast Candida utilis AS2.1180 compatibility, mixed strains also adds photosynthetic bacterium, saccharifying enzyme, milk-acid bacteria is worked in coordination with the unsterilised fermentation of biosolids:
1) the solid fermentation material formula is represented with weight percent:
80% agricultural crop straw powder, 15% wheat bran, 12% Semen Maydis powder, 0.3% urea, 0.5% dipotassium hydrogen phosphate, 0.5% magnesium chloride, 0.4% sodium-chlor is with phosphoric acid regulating ph value about 5.5;
2) bacterial classification access amount is that 100% weight is benchmark in culture material:
Fungi aspergillus niger AS3.350 solid seed 5%, yeast Candida utilis AS2.1180 seed liquor 20%, photosynthetic bacterium seed liquor 5%, saccharifying enzyme 0.5%, milk-acid bacteria 0.3%;
3) inoculation and fermentation:
Bacterial classification is inserted in the culture material, stir, insert in fermentation machine or the fermentation vat, under 28-32 ℃ of temperature, cultivated 48-72 hour, get final product preparing complete feed or make particle oven dry storage;
3. yeast geotrichum candidum AS2.361 and viride AS3.2928 and mould AS3.287 compatibility mixed strains, and add photosynthetic bacterium, the collaborative grog biosolids fermentation of sterilizing of high-activity yeast bacterium:
1) the solid fermentation material formula is represented with weight percent:
95% agricultural crop straw powder, 2% wheat bran or Semen Maydis powder, 0.5% urea, 0.3% calcium superphosphate, 0.5% bone meal, 0.3% sodium-chlor, with phosphoric acid regulating ph value about 5.5, normal-pressure sterilization 30 minutes, cool to 45 ℃ after inoculation;
2) bacterial classification access amount is that 100% weight is benchmark in culture material:
Geotrichum candidum AS2.361 seed liquor 10%, viride AS3.2928 solid seed 0.5%, mould AS3.287 solid seed 0.7%, photosynthetic bacterium seed liquor 1%, high-activity yeast 0.01%;
3) inoculation and fermentation:
Bacterial classification is inserted in the culture material, stir, insert in fermentation machine or the fermentation vat, under 28-32 ℃ of temperature, cultivated 48-72 hour, get final product preparing complete feed or make particle oven dry storage;
Need to prove the collection that is numbered Institute of Microorganism, Academia Sinica number behind the strain name.
In this case, fermentation machine, fermentor tank, fermentation container, fermentation vat are the container of fermentation usefulness, have identical effect.
Subordinate list 1,2 and 3 has illustrated the composition of feed of the manufacturing of adopting present method and the effect of raising, and table 4 and 5 have provided the biological fodder that method of the present invention produces and the prescription under the different application condition of complete feed.
Table 1: sample title: farm crop straw organism fermented feed sample number into spectrum: S950758
Detect unit: State Fodder Quality Supervision ﹠ Examination Center (Beijing);
| Interventions Requested unit | Assay | The method of inspection |
| Crude protein % | 10．4 | GB6432-86 |
| Robust fibre % | 19．41 | GB6434-86 |
| Phosphorus % | 0．303 | GB6437-86 |
| Iron mg/kg | 2．2*10 | BG1T13885-92 |
| VB2 mg／kg | 43．0 | GB1T14701-93 |
| Crude fat % | 0．59 | GB6433-86 |
| Total reducing sugar % | 19．8 | Instrument |
| Heat energy kj/100g | 1218 | Instrument |
| Aflatoxin Bi ug/kg | 18．8 | Instrument |
| Aspartic acid | 1．10 | Ion-exchange chromatography |
| Threonine | 0．57 | Ion-exchange chromatography |
| Serine | 0．56 | Ion-exchange chromatography |
| L-glutamic acid | 1．50 | Ion-exchange chromatography |
| Proline(Pro) | 0．94 | Ion-exchange chromatography |
| Glycine | 0．58 | Ion-exchange chromatography |
| L-Ala | 0．73 | Ion-exchange chromatography |
| Interventions Requested | Assay | Assay |
| Gelucystine | ／ | Ion-exchange chromatography |
| Xie Ansuan | 0．73 | Ion-exchange chromatography |
| Methionine(Met) | 0．10 | Ion-exchange chromatography |
| Isoleucine | 0．66 | Ion-exchange chromatography |
| Leucine | 0．86 | Ion-exchange chromatography |
| Tyrosine | 0．30 | Ion-exchange chromatography |
| Phenylalanine | 0．88 | Ion-exchange chromatography |
| Methionin | 0．62 | Ion-exchange chromatography |
| Histidine | 0．22 | Ion-exchange chromatography |
| Arginine | 0．72 | Ion-exchange chromatography |
The analyzer room, Beijing City Nutrient Source Inst
Table 2: the farm crop straw organism feed laying hen effect test group (15) of feeding
| The test fate | Lay eggs (number) | Laying rate (%) | Egg size g/ number | Usage ratio (%) | Feed consumption g/ only | Damaged (number) | Color and luster |
| 1 | 11 | 73．3 | 59．09 | 30 | 140 | 0 | Deeply |
| 2 | 12 | 80 | 60．42 | 30 | 140 | 0 | Deeply |
| 3 | 13 | 86．67 | 61．54 | 30 | 133．3 | 0 | Deeply |
| 4 | 15 | 100 | 63．33 | 30 | 140 | 0 | Deeply |
| 5 | 12 | 80 | 58．33 | 40 | 140 | 0 | Deeply |
| 6 | 12 | 80 | 60．42 | 40 | 140 | 0 | Deeply |
| 7 | 11 | 73．33 | 59．09 | 40 | 140 | 0 | Deeply |
| 8 | 13 | 86．67 | 65．38 | 50 | 140 | 0 | Deeply |
| 9 | 13 | 86．67 | 65．38 | 60 | 133．3 | 0 | Deeply |
| 10 | 12 | 80 | 60．42 | 70 | 133．3 | 0 | Deeply |
| 11 | 12 | 80 | 60．42 | 70 | 133．3 | 0 | Deeply |
| 12 | 14 | 93．33 | 64．29 | 70 | 133．3 | 0 | Deeply |
| 13 | 11 | 73．33 | 63．64 | 70 | 130 | 0 | Light |
| 14 | 12 | 80 | 60．42 | 70 | 133．3 | 0 | Deeply |
| 15 | 12 | 80 | 60．42 | 80 | 133．3 | 1 | Deeply |
| 16 | 12 | 80 | 60 | 80 | 133．3 | 1 | Deeply |
| 17 | 11 | 73．33 | 61．3 | 90 | 133．3 | 0 | Deeply |
| 18 | 10 | 66．67 | 65 | 100 | 126．7 | 0 | Deeply |
| 19 | 8 | 53．33 | 62．5 | 100 | 126．7 | 1 | Deeply |
| 20 | 8 | 53．33 | 62．5 | 100 | 126．7 | 0 | Deeply |
| 234 | 1233．89 | 3 | Deeply |
Continuous table 2: control group (15)
Annotate: (1) usage ratio is 70% the occupancy volume of corn in complete feed.
| The test fate | Lay eggs (number) | Laying rate (%) | Egg size g/ number | Usage ratio (%) | Feed consumption g/ only | Damaged (number) | Color and luster |
| 1 | 11 | 73．3 | 63．64 | 30 | 133．33 | 1 | Deeply |
| 2 | 12 | 80 | 62．5 | 30 | 133．3 | Do not have | Deeply |
| 3 | 12 | 80 | 62．5 | 30 | 133．3 | 1 | Shallow |
| 4 | 73．3 | 59．09 | 30 | 133．3 | 1 | Shallow | |
| 5 | 13 | 86．67 | 65．38 | 40 | 133．3 | 1 | Shallow |
| 6 | 12 | 80 | 62．5 | 40 | 133．3 | Do not have | Deeply |
| 7 | 12 | 80 | 62．5 | 40 | 133．3 | Do not have | Deeply |
| 8 | 10 | 66．67 | 60 | 50 | 133．3 | Do not have | Deeply |
| 9 | 11 | 73．3 | 63．48 | 60 | 133．3 | Do not have | Shallow |
| 10 | 12 | 80 | 60．42 | 70 | 133．3 | Do not have | Shallow |
| 11 | 11 | 73．3 | 59．09 | 70 | 133．3 | Deeply | |
| 12 | 12 | 80 | 60．42 | 133．3 | Do not have | Deeply |
| 13 | 11 | 73．3 | 59．09 | 70 | 133．3 | Do not have | Light |
| 14 | 10 | 66．67 | 57．5 | 70 | 133．3 | Do not have | Deeply |
| 15 | 12 | 80 | 60．42 | 80 | 133．3 | Do not have | Deeply |
| 16 | 8 | 53．33 | 62．5 | 80 | 133．3 | Do not have | Deeply |
| 17 | 11 | 73．3 | 59．09 | 90 | 133．3 | Do not have | Deeply |
| 18 | 11 | 73．3 | 61．36 | 100 | 130 | 1 | Deeply |
| 19 | 9 | 60 | 61．11 | 100 | 133．3 | Do not have | Deeply |
| 20 | 11 | 73．3 | 59．09 | 100 | 133．3 | Do not have | Deeply |
| 222 | 1221．84 | 7 | Deeply |
(2) test site is in poulty house, eastern suburb, Datong City, Shanxi Province.Time: 10 ,-May 1,95 on April.
(3) examination is fed group than 12 on control group fecund egg, and examination is fed the group breakage and lacked 4 than control group again.
(4) 20 days eggs of test group add up to: 1233.89g, and control group is: 1221.84g,
The test group 12.05g that increases weight more.
(5) examination hello group crop material feed occupancy volume is 49%, and the crop material feed cost is by 1.0 yuan/kg
Calculate, corn calculates by 1.4 yuan/kg, and 20 days test group are fed feed from examination and can be saved 0.5 yuan
(6) see that from the color of laying eggs test group is generally better than control group.Table 3: farm crop straw organism fermented feed growing and fattening pigs test: table 3-1: the feeding crop material of test pig is biological to cooperate complete feed and control group weightening finish situation relatively:
(1) test period:, amount to 90 days from end of day in 1, to 95 year August 1 of 95 on May.(2) test site: Fu Zhuan pig farm, rice field town, Fengnan, Tangshan City.(3) source of test pig and grouping:
The growing and fattening pigs of rice field, Fengnan town Fu Qian village plant are all adopted in this test.Table 3-2: biological complete feed and the control group feed consumption situation cost table of cooperating of the feeding crop material of test pig
Cooperate complete feed feed growing and fattening pigs test and the average 3.5kg of control group quantitative feeding every day
By table 3-2 as can be seen, test group and control group are under the situation of equivalent amount feed of feeding, the weightening finish situation is basic identical, test group was than the low 450g of control group weightening finish in 90 days, see by control group and test group feed price, the every kg feed of test group and control group reduces cost 0.2 yuan, and therefore, the examination of 90 day trial period is fed group and reduce cost 63 yuan than control group from feed cost.
Table 4: farm crop straw organism complete feed prescription (chicken feed) the reserve chicken feed layer chicken feed broiler fodder of laying eggs
| Raw material | Ratio | Albumen % | Ratio | Albumen | Ratio | Albumen % |
| Biological fodder | 40 | 3．2 | 50 | 3．2 | 40 | 2．8 |
| Corn | 28 | 2．24 | 25 | 2．4 | 25 | 2．4 |
| Wheat bran | 10 | 1．57 | 8 | 1．12 | ||
| Soya-bean cake | 8 | 3．2 | 10 | 4 | 13 | 5．2 |
| Fish meal | 3 | 1．65 | 3 | 1．65 | 3 | 1．65 |
| Bone meal | 1 | 1 | 1 | |||
| Oyster shell whiting | 1 | 2 | 1 | |||
| Zeolite powder | 1 | 1 | 1 | |||
| Salt | 0．5 | 0．5 | 0．5 | |||
| Infinitesimal | 1 | 1 | 1 | |||
| Yeast | 3 | 1．56 | 3 | 1．56 | 3 | 1．56 |
| Terramycin | 0．004 | 0．004 | 0．004 | 0．004 | ||
| Feather meal | 3 | 2．3 | 3 | 2．3 | 3 | 2．3 |
Table 5: farm crop straw organism complete feed prescription (pig's feed) pig starter feed store pig
| Raw material | Ratio | Albumen % | Ratio | Albumen % |
| Biological fodder | 40 | 3．2 | 50 | 4 |
| Corn | 28 | 2．24 | 18 | 1．4 |
| Wheat bran | 10 | 1．57 | 10 | 1．57 |
| Soya-bean cake | 20 | 8 | 8 | 3．2 |
| Fish meal | 3 | 1．65 | 3 | 1．65 |
| Bone meal | 1 | 1 | ||
| Oyster shell whiting | 1 | 1 | ||
| Zeolite powder | 1 | 1 | ||
| Salt | 0．5 | 0．5 | ||
| Infinitesimal | 1 | 1 | ||
| Yeast | 3 | 1．56 | 3 | 1．56 |
| Asccharin | 0．0002 | 0．0002 | ||
| Essence | 0．0002 | 0．0002 | ||
| Terramycin | 0．004 | 0．004 | 0．0 | |
| Feather meal | 3 | 2．3 | 3 | 2．3 |
Claims (1)
1, a kind of production method of biological multi-bacterial fermented fodder made from stalks comprises the preparation of raw material, comprises the preparation of culture material and the preparation of bacterial classification, separates purifying; Bacterial classification is inserted in the culture material, ferment; After fermenting, get final product preparing complete feed or granulate the oven dry storage;
Wherein the preparation of culture material is expressed as with weight percent:
Crop material was pulverized 5～25 orders, and the straw powder with 80～95% adds wheat bran 0～15%, Semen Maydis powder 0～12%, urea 0～1%, calcium superphosphate 0～0.8%, sodium-chlor 0～1%, bone meal 0～1%, dipotassium hydrogen phosphate 0～1%, ammonium chloride 0～0.3%, magnesium chloride 0～0.8% is transferred pH value to 4.5～5.5 with phosphoric acid, normal-pressure sterilization half an hour or unsterilised, cool to 25～35 ℃;
The bacterial classification that inserts in the culture material is that 100% weight is that benchmark is counted with culture material:
Yeast geotrichum candidum AS2.498 seed liquor 6～14% and viride AS3.3032 solid seed 0.5～2% compatibility; Or
Fungi aspergillus niger AS3.350 solid seed 3～6% and yeast Candida utilis AS2.1180 seed liquor 15～25% compatibilities; Or
Yeast geotrichum candidum AS2.361/AS2.498 seed liquor 6～13% and viride AS3.2928 solid seed 0.2～1.2% and mould AS3.287 solid seed 0.4～1.0% compatibility;
More than the bacterial classification of various compatibilities add liquid photosynthetic bacterium seed liquor 0～5% again, high-activity yeast bacterium 0～0.02%, saccharifying enzyme 0～0.8% milk-acid bacteria 0～0.5%;
According to quantity bacterial classification is joined in the culture material, mix, insert fermentor tank or fermentation machine or fermentation vat, cultivated 40～80 hours down for 25～35 ℃ in temperature.
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| CN101313728B (en) * | 2008-05-17 | 2011-09-21 | 邹宗森 | Stalk biochemistry forage grass and its processing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1101673A (en) * | 1993-10-13 | 1995-04-19 | 傅为民 | Microorganism fermenting compound fungus and method for fermenting crop stem to produce livestock fodder |
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| CN1101673A (en) * | 1993-10-13 | 1995-04-19 | 傅为民 | Microorganism fermenting compound fungus and method for fermenting crop stem to produce livestock fodder |
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| CN101313728B (en) * | 2008-05-17 | 2011-09-21 | 邹宗森 | Stalk biochemistry forage grass and its processing method |
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