CN1214856A - Breeding method of rice sterile line and its heterosis combination - Google Patents
Breeding method of rice sterile line and its heterosis combination Download PDFInfo
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- CN1214856A CN1214856A CN 98124891 CN98124891A CN1214856A CN 1214856 A CN1214856 A CN 1214856A CN 98124891 CN98124891 CN 98124891 CN 98124891 A CN98124891 A CN 98124891A CN 1214856 A CN1214856 A CN 1214856A
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- sterile line
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 32
- 235000009566 rice Nutrition 0.000 title claims abstract description 32
- 240000007594 Oryza sativa Species 0.000 title claims 2
- 238000009395 breeding Methods 0.000 title abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 12
- 238000012360 testing method Methods 0.000 claims abstract description 8
- 238000009396 hybridization Methods 0.000 claims abstract description 7
- 206010000210 abortion Diseases 0.000 claims abstract description 4
- 231100000176 abortion Toxicity 0.000 claims abstract description 4
- 238000012423 maintenance Methods 0.000 claims description 10
- 238000011084 recovery Methods 0.000 claims description 9
- 230000008774 maternal effect Effects 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 238000012797 qualification Methods 0.000 claims description 4
- 230000008775 paternal effect Effects 0.000 claims description 3
- 241000209094 Oryza Species 0.000 abstract description 30
- 238000009401 outcrossing Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 235000013339 cereals Nutrition 0.000 description 4
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 235000021329 brown rice Nutrition 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- YNPNZTXNASCQKK-UHFFFAOYSA-N phenanthrene Chemical compound C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000002362 mulch Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A method for selectively culturing the sterile line and hybridized rice features that the male parent (L301) and the female parent (881) are hybridized to each other, and the male parent (F) is hybridized to each other2Generation selection of sterile plants with complete abortion, F297X 959 hybridization2Crossing the excellent single plants, testing the sterile plants in pairs, backcrossing continuously for 10 generations to obtain Chuan 7A, and preparing the hybrid seeds with Chuan 7A as female parent and the existing restoring line as male parent. The sterile line has excellent rice quality, high stigma exposure rate and good outcrossing seed setting rate, the breeding seed production can reach 220-250 kg/mu, the yield is 50-70 kg more than that of Zhenshan 97A, and the yield of the combined hybrid-optimal combination is increased by 8.5 percent than that of Shanyou 63.
Description
The invention belongs to crop hybrid superiority and utilize technical field, be specifically related to the selection of high different friendship male sterile line of hybrid rice high-quality and assorted excellent combination.
Hybrid rice is applied in China, but there are many shortcomings in existing rice sterile line, causes further developing of hybrid rice to be subjected to very big restriction.(1), make the rice matter of existing hybrid rice can not satisfy the requirement that people's living standard improves day by day because rice sterile line rice matter is relatively poor; (2) the rice sterile line outcrossing seed-setting rate is low, makes that the seed production cost is too high, has increased the weight of peasant's the burden and the market risk of seed enterprise.Thereby high-quality, the high different friendship male sterile line of the rice district plantation of seed selection suitable China south are great research topics on the crops good variety selection always.
The objective of the invention is to solve the problems referred to above in the hybrid rice production of rice district, China south, select wide adaptability, rice is of fine quality, the outcrossing seed-setting rate height, and the high yield that plant and leaf shape is good, the rice sterile line of high-combining ability, and utilize the strong advantage combination of this male sterile line assembly.
The object of the present invention is achieved like this:
The selection of a kind of hybrid rice high yield, disease-resistant, of fine quality male sterile line and assorted excellent combination is maternal with L301, and maintenance line 881 is a paternal hybrid, F
2In generation, selected the sterile completely strain of abortion, with the F of maintenance line D297 * maintenance line 959 hybridization
2The hybridization of Dai Zhongxuan elite seedling, the paired test cross of individual plant is carried out in the sterile strain of filial generation, and in continuous 10 generations, backcross according to qualifications, breeds male sterile line river 7A; With male sterile line river 7A is maternal, and existing recovery is a male parent, the preparing hybrid kind.
Recovery is CDR917.
Recovery is CDR909.
Recovery is R19.
A kind of hybrid rice high yield, disease-resistant, the seed selection of male sterile line of fine quality and breeding and the seeding technique of crossbreed thereof are realized by following steps.
1, be maternal with L301 and free new maintenance line 881 is a paternal hybrid, F
2In generation, isolates sterile strain and can educate two types of strains, and the fertility abortion of its sterile strain is thorough, does not dye through microscopy.Again from the F of high-quality maintenance line D297 * long stigma maintenance line 959 hybridization
2It is normally solid for Dai Zhongxuan, and elite seedling that plant and leaf shape is good and L301/881 are hybridized isolated sterile strain and carried out the paired test cross of individual plant, continuous 10 generation (F
10Generation) backcrosses according to qualifications.It is good to have bred good grain quality, stigma exposing ratio height, flowering habit, outcrossing seed-setting rate height, the new sterile line river 7A that coordinate force is strong.
2, be female parent with this sterile river 7A, it is male parent that existing high-quality is recovered, and assembly goes out high yield, strong advantage series of combination disease-resistant, of fine quality.
Fig. 1 is male sterile line of the present invention river 7A and assorted excellent combination breeding line spectrogram thereof
Advantage of the present invention and good effect are as follows:
1, rice is of fine quality: paddy rice of the present invention is not bred river 7A, and rice is of fine quality in present production Zhenshan 97a, hilllock 46A that large tracts of land is used. The length-width ratio of this sterile line=3: 1, rice Grain is translucent, and a small amount of white belly is arranged, no core white, and food flavor is better, its marketing quality and nutritional quality All be better than Zhenshan 97a and hilllock 46A.
2, stigma exposing ratio height, outcrossing seed-setting rate are good: the sterile line river 7A that the present invention breeds Stigma exposing ratio is 92.5%, and two exposing accounts for 51.9%, and is higher by 45.2% than Zhenshan 97a, by In the stigma exposing ratio height, normal during flower, outcrossing habit is good, general outcrossing than Zhenshan 97a The high 15-20% of setting percentage is through test, the general per mu yield 220-of this sterile line propagation and the production of hybrid seeds 250 kilograms, high reaches 280 kilograms, than every mu of Zhenshan 97a breeding and receives the 50-70 public affairs Jin.
3, strong stress resistance, Comprehensive Traits are good: the sterile line river 7A that the present invention breeds, in anti-Rice blast, it is better that institute's combo is closed field resistance. Well developed root system, cane are sturdy, blade thick straight, Proper height, ripe phase suit, and the later stage, ripe look good.
4, wide adaptability, yield potential are big: the sterile line river 7A that the present invention breeds is general Make rice growing in the season, date of seeding, is identical with Zhenshan 97a, and breeding time is longer 2 days than Zhenshan 97a. Add that disease resistance is strong, so the suitable planting scope is wide, can be at south China medium rice district treasure Shan The plantation of 97A crossbreeding area. Excellent 917 (the river 7A/CDR917) in river: through Shen in 1998 Ask someone to test 589.6 kilograms of per mu yields, were Shanyou 63 volume increase 8.5%.
Specific embodiments of the invention are described in detail in detail below:
Parental source and feature thereof:
Male sterile line of the present invention river 7A system selects L301B * 881B//No. 5 * 68 hybridization early of red reality for use.At F
2Isolate sterile strain in generation and can educate strain.Backcross with filial generation and the aforementioned sterile strain test cross of D297B * 959B//(phenanthrene changed for 112/ morning 68) in the back, combine male sterile line A and corresponding maintenance line B, carrying out resistance when male sterile line is backcrossed identifies, identify and the combining ability test result according to resistance, and field performance, backcrossing according to qualifications continuously, isozygotying through 10 generations, bred the male sterile line river 7A of high-quality, high different friendship, this male sterile line is that a comprehensive proterties is good, the stigma exposing ratio height, flowering habit is good, and the ripe phase is suitable, proper height, the high-combining ability male sterile line that rice is of fine quality.
The feature of river 7A: be indica type, Chengdu sowing mid-April, be 116 days breeding time, grow 2 days than the Zhenshan 97a of broadcasting the same period, it is 131 that 91 centimetres of this male sterile line plant heights, 23.5 centimetres of spike lengths, every fringe grain, stigma exposing ratio is 92.5%, two exposing accounts for 51.9%, and be normal during flower, the outcrossing seed-setting rate height, its sterile degree and sterile plant rate average out to 100%, seedling stage, growing way was prosperous, and plant strain growth is neat, and the stem blade is 13 leaves, the bran point is coloured, no awns, the fringe layer is neat, quality better, brown rice yield 81%, polished rice rate 79%, middle blast resisting, wide adaptability can be at southern rice district crossbreeding.
With river 7A is female parent, and existing recovery is a male parent, and the new combination that assembly goes out has: combinations such as river 7A/CDR917, river 7A/CDR909, river 7A/R19.The synthetic all area sowings in the first tenday period of a month in April (two sections seedlings of mulch film) of new combo are about the 150 days time of infertility, suitable breeding time with Shanyou 63, seedling stage growing way prosperous, tiller medium on the upper side, plant type tightly loose moderate, neat, the ripe look of fringe layer is good, the yellow bar green grass or young crops of paddy, disease resistance is strong, ripening rate is high, thousand kernel weight is the 27.5-28.5 gram, brown rice yield 81.0%, and the grain of rice is translucent, food flavor is good, in blast resisting, wide adaptability can be in Sichuan and southern main rice district popularizing planting.
Claims (4)
1, the selection of a kind of hybrid rice high yield, disease-resistant, of fine quality male sterile line and assorted excellent combination is characterized in that: be maternal with L301, maintenance line 881 is a paternal hybrid, F
2In generation, selected the sterile completely strain of abortion, with the F of maintenance line D297 * maintenance line 959 hybridization
2The hybridization of Dai Zhongxuan elite seedling, the paired test cross of individual plant is carried out in the sterile strain of filial generation, and in continuous 10 generations, backcross according to qualifications, breeds male sterile line river 7A; With male sterile line river 7A is maternal, and existing recovery is a male parent, the preparing hybrid kind.
2, selection according to claim 1 is characterized in that: recovery is CDR917.
3, selection according to claim 1 is characterized in that: recovery is CDR909.
4, selection according to claim 1 is characterized in that: recovery is R19.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98124891 CN1110245C (en) | 1998-12-03 | 1998-12-03 | Breeding method of rice sterile line and its heterosis combination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 98124891 CN1110245C (en) | 1998-12-03 | 1998-12-03 | Breeding method of rice sterile line and its heterosis combination |
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| Publication Number | Publication Date |
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| CN1214856A true CN1214856A (en) | 1999-04-28 |
| CN1110245C CN1110245C (en) | 2003-06-04 |
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| CN 98124891 Expired - Fee Related CN1110245C (en) | 1998-12-03 | 1998-12-03 | Breeding method of rice sterile line and its heterosis combination |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
| CN108040869A (en) * | 2017-12-11 | 2018-05-18 | 安徽荃银高科种业股份有限公司 | The two-line sterile line selection of multiple gene polymerization used in a kind of suitable for mechanization Mixed plant |
| CN110506626A (en) * | 2019-09-29 | 2019-11-29 | 四川农业大学 | A kind of selection of high-quality odor type CMS line I6A |
| CN110506625A (en) * | 2019-09-29 | 2019-11-29 | 四川农业大学 | A kind of I2A selection of high-quality CMS line |
-
1998
- 1998-12-03 CN CN 98124891 patent/CN1110245C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104686320A (en) * | 2015-04-01 | 2015-06-10 | 内江杂交水稻科技开发中心 | Cultivation method of aromatic high-quality rice sterile line |
| CN108040869A (en) * | 2017-12-11 | 2018-05-18 | 安徽荃银高科种业股份有限公司 | The two-line sterile line selection of multiple gene polymerization used in a kind of suitable for mechanization Mixed plant |
| CN110506626A (en) * | 2019-09-29 | 2019-11-29 | 四川农业大学 | A kind of selection of high-quality odor type CMS line I6A |
| CN110506625A (en) * | 2019-09-29 | 2019-11-29 | 四川农业大学 | A kind of I2A selection of high-quality CMS line |
| CN110506625B (en) * | 2019-09-29 | 2021-07-06 | 四川农业大学 | I2A breeding method of high-quality three-line sterile line |
| CN110506626B (en) * | 2019-09-29 | 2021-07-06 | 四川农业大学 | Breeding method of high-quality fragrant three-line sterile line I6A |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1110245C (en) | 2003-06-04 |
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