CN110506626B - Breeding method of high-quality fragrant three-line sterile line I6A - Google Patents

Breeding method of high-quality fragrant three-line sterile line I6A Download PDF

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CN110506626B
CN110506626B CN201910935061.XA CN201910935061A CN110506626B CN 110506626 B CN110506626 B CN 110506626B CN 201910935061 A CN201910935061 A CN 201910935061A CN 110506626 B CN110506626 B CN 110506626B
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fragrant
line
quality
sterile
single plant
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CN110506626A (en
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刘怀年
李平
邓晓建
孙永健
邓其明
郑爱萍
王世全
李双成
朱军
梁越洋
马均
王玲霞
京金花
邹挺
刘尧
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Sichuan Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a breeding method of a high-quality fragrant three-line sterile line I6A, which comprises the steps of carrying out artificial hybridization by taking fragrant type Heiyou D1B as a female parent and taking gold 23B as a male parent, carrying out 3-generation system breeding to breed fragrant single plants with excellent plant types, excellent comprehensive properties and rice blast resistance, then carrying out multi-generation continuous backcross with Jing 46A, carrying out generation-by-generation microscopic examination and selection in the backcross process, selecting single plants with completely abortive fertility and basically consistent plant types and male parents in sterile line backcross groups, carrying out bidirectional selection, shortening the breeding period, finally breeding to breed a three-line fragrant sterile line with stable fertility, excellent rice quality, high breeding seed production yield, good matched combined resistance, strong hybrid vigor and good comprehensive properties, and is named as a high-quality three-line sterile line I6A. The combined rice of the invention has excellent quality and high yield, really realizes the organic combination of high quality and high yield, and solves the problem of high yield but poor quality of hybrid rice in rice breeding.

Description

Breeding method of high-quality fragrant three-line sterile line I6A
Technical Field
The invention belongs to the technical field of rice breeding, and particularly relates to a breeding method of a high-quality fragrant three-line sterile line I6A.
Background
Since the three-line hybrid rice is matched in 1973, the three-line hybrid rice is popularized in a large scale in the country in 1976, the rice yield in China continuously steps, and huge social benefits and economic benefits are generated. New sterile lines are continuously developed, such as Zhenshan 97A, D SHA A, Ongkang 46A, Xieqingzao A, II-32A, V20A and the like, and play a great role in rice production. Because the rice quality of the sterile lines is poor and the sterile lines are applied for many years in production, main diseases in production are easy to infect, and the yield of the matched rice variety is in a wandering situation. The high-yield rice variety is poor in rice quality, the yield of the good rice variety is not high, in order to solve the contradiction between high yield and high quality, the high-quality and high-yield of the two-line hybrid rice in Hunan walks at the front of the whole country, the two-line hybrid rice mainly comprising the Y58S female parent is bred, the popularization area is large, but rice blast is easy to occur due to long popularization age, the seed production fertility is not safe enough in the great agricultural province of Sichuan, and the rice quality also has the requirement for further improvement.
D fragrance 1B rice is excellent in quality and has fragrance, but the fertility of Sichuan is not improved due to unstable fertility, incomplete abortion, long flag leaves with high combination, and the like, and the combination is not pushed away in a large area in production.
Therefore, there is a need to provide a method for breeding a three-line sterile line with excellent rice quality, good resistance, excellent plant type and safe seed production fertility, and simultaneously, a rice sterile line (variety) with high yield, good quality, excellent plant type and environmental protection.
Disclosure of Invention
In view of the above, the invention provides a breeding method of a high-quality fragrant three-line sterile line I6A, aiming at the problems of poor rice quality, aging, wandering first-generation seed yield and unsafe fertility of a two-line sterile line in Sichuan.
In order to solve the technical problem, the invention discloses a breeding method of a high-quality fragrant three-line sterile line I6A, which comprises the following steps:
step 1, selecting D fragrance 1B containing fragrance type blood relationship as a female parent and gold 23B as a male parent to carry out artificial hybridization, and harvesting hybrid seeds;
step 2, planting hybrid F1Through 2 generations of systematic breeding, a single plant F with excellent rice quality, good resistance, excellent plant type, good comprehensive character and rice blast resistance is bred3The single plant is basically stable and contains fragrance;
step 3, using the gamboge 46A as a female parent, and hybridizing the maintainer line D, the incense 1B and the gold 23B3The fragrant later generation is used as a male parent to prepare a hybrid combination to obtain A1F1(ii) a Planting F3Fragrant single plant, harvesting fragrant single plant seed F4；
Step 4, in F4In the population, selection of individual plants F prone to the trait of interest4And with hit and select A1F1Hybridizing sterile single plants in pairs; preparing hybrid A2F1(ii) a Planting F4Fragrant single plant, harvesting fragrant single plant seed F5；
Step 5, in F5In the population, selection of individual plants F prone to the trait of interest5And with hit and select A2F1The sterile single plants are hybridized in pairs to prepare hybrid A3F1(ii) a Planting F5Fragrant single plant, harvesting fragrant single plant seed F6；
Step 6, in F6In the population, selection of individual plants F prone to the trait of interest6And with hit and select A3F1Sterile sheetCrossing the plants in pairs to prepare hybrid A4F1Namely a high-quality fragrant three-line sterile line I6A with a planting fragrance F6Harvesting the fragrant seeds F7I.e., retention system I6B.
Optionally, the single plant with the target property specifically refers to a single plant with good plant type, more tillers, slender grain shape, and more consistent properties of a plurality of single plants, and has fragrance.
Optionally, the selection criteria of the sterile individuals in the step 4 and the step 5 are: the white thin anther is observed by naked eyes, and then the single plant with classical failure is determined as the sterile single plant.
Compared with the prior art, the invention can obtain the following technical effects:
1) the invention has advanced technical route and bidirectional selection of parents and parents;
2) the processing quality of the high-quality fragrant three-line sterile line bred by the invention is as follows: the brown rice rate is 79.8 percent, the polished rice rate is 70.2 percent, and the whole polished rice rate is 55.6 percent; appearance quality: chalkiness rate 9.7%, rice grain length 7.7mm, length-width ratio 3.4; the quality of the cooked food is as follows: the amylose content is 14%, the gelatinization temperature is 4.1, the consistency of the gel is 90mm, and the nutrition quality is as follows: the protein content was 8.1%.
3) The combined rice of the invention has excellent quality and high yield, really realizes the organic combination of high quality and high yield, and solves the problem of high yield but poor quality of hybrid rice in rice breeding.
Of course, it is not necessary for any one product in which the invention is practiced to achieve all of the above-described technical effects simultaneously.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a flow chart of the breeding method of the high-quality fragrant three-line sterile line I6A.
Detailed Description
The following embodiments are described in detail with reference to the accompanying drawings, so that how to implement the technical features of the present invention to solve the technical problems and achieve the technical effects can be fully understood and implemented.
The invention also discloses a breeding method of a high-quality fragrant three-line sterile line I6A, as shown in figure 1, using D fragrant 1B (containing Basmati fragrant blood relationship) bred by Sichuan agriculture university rice institute as a female parent (mentioned in the identification of D fragrant 4A (variety weight: CNA20060666.2) variety), gold 23B (sold in market) as a male parent to perform artificial hybridization, performing 2-generation system breeding to breed a single plant with excellent plant type, excellent comprehensive character and rice blast resistance, performing multi-generation continuous backcross with Oak 46A (sold in market) afterwards, performing generation-by-generation microscopic examination and selection in the backcross process, selecting a single plant with excellent plant type and male parent basically consistent with each other in sterile line backcross population, performing bidirectional selection, shortening breeding time limit, and finally breeding to breed stable and fragrant single plants, The three-line fragrant type sterile line with excellent rice quality, high breeding seed yield, good resistance of matched combination, strong heterosis and good comprehensive characters is named as a high-quality fragrant type three-line sterile line I6A, and comprises the following steps:
step 1, selecting D fragrance 1B containing fragrance type blood relationship as a female parent and gold 23B as a male parent to carry out artificial hybridization, and harvesting hybrid seeds;
step 2, planting hybrid F1Through 2 generations of systematic breeding, a single plant F with excellent rice quality, good resistance, excellent plant type, good comprehensive character and rice blast resistance is bred3The single plant is basically stable and contains fragrance;
step 3, using the gamboge 46A as a female parent, and hybridizing the maintainer line D, the incense 1B and the gold 23B3The fragrant later generation is used as a male parent to prepare a hybrid combination to obtain A1F1(ii) a Planting F3Fragrant single plant, harvesting fragrant single plant seed F4；
Step 4, in F4In the population, selecting a single plant F with good plant type, more tillers, slender grain shape, consistent properties of a plurality of single plants and fragrance4And with hit and select A1F1Sterile Single plant (white and thin anther will be observed by naked eye)Then the single plant with classical failure is determined as sterile single plant) are paired and crossed to prepare hybrid A2F1(ii) a Planting F4Fragrant single plant, harvesting fragrant single plant seed F5；
Step 5, in F5In the population, selecting a single plant F with good plant type, more tillers, slender grain shape, consistent properties of a plurality of single plants and fragrance5And with hit and select A2F1Crossing sterile individual plants (white thin anther observed by naked eye, and identifying the individual plants with classical failure by microscopic examination as sterile individual plants) in pairs to obtain hybrid A3F1(ii) a Planting F5Fragrant single plant, harvesting fragrant single plant seed F6；
Step 6, in F6In the population, selecting a single plant F with good plant type, more tillers, slender grain shape, consistent properties of a plurality of single plants and fragrance6And with hit and select A3F1Crossing sterile individual plants (white thin anther observed by naked eye, and identifying the individual plants with classical failure by microscopic examination as sterile individual plants) in pairs to obtain hybrid A4F1Namely a high-quality fragrant three-line sterile line I6A with a planting fragrance F6Harvesting the fragrant seeds F7I.e., retention system I6B.
At this time, the whole process of breeding the sterile line I6A is basically finished, and the sterile line I6A and the maintainer line I6B are completely stable.
Example 1
Selecting D fragrance 1B containing fragrance type blood relationship as female parent and gold 23B as male parent to carry out artificial hybridization, and harvesting hybrid seeds;
planting hybrid F1Through 2 generations of systematic breeding, a single plant F with excellent rice quality, good resistance, excellent plant type, good comprehensive character and rice blast resistance is bred3The single plant is basically stable and contains fragrance;
in Wenjiang in Sichuan in 2010, using Okawa 46A as female parent and maintainer line D, Xiang 1B and Jin23B hybrid F3The fragrant later generation is used as a male parent to prepare a hybrid combination to obtain A1F1(ii) a Planting F3Fragrant single plant, harvesting fragrant single plant seed F4；
2010Annual Hainan Ling water, in F4In the population, selecting a single plant F with good plant type, more tillers, slender grain shape, consistent properties of a plurality of single plants and fragrance4And with hit and select A1F1Crossing sterile individual plants (white thin anther observed by naked eye, and identifying the individual plants with classical failure by microscopic examination as sterile individual plants) in pairs to obtain hybrid A2F1(ii) a Planting F4Fragrant single plant, harvesting fragrant single plant seed F5；
Wen Jiang in Sichuan in 2011, and F5In the population, selecting a single plant F with good plant type, more tillers, slender grain shape, consistent properties of a plurality of single plants and fragrance5And with hit and select A2F1Crossing sterile individual plants (white thin anther observed by naked eye, and identifying the individual plants with classical failure by microscopic examination as sterile individual plants) in pairs to obtain hybrid A3F1(ii) a Planting F5Fragrant single plant, harvesting fragrant single plant seed F6；
In 2011 in Hainan Ling water, in F6In the population, selecting a single plant F with good plant type, more tillers, slender grain shape, consistent properties of a plurality of single plants and fragrance6And with hit and select A3F1Crossing sterile individual plants (white thin anther observed by naked eye, and identifying the individual plants with classical failure by microscopic examination as sterile individual plants) in pairs to obtain hybrid A4F1Harvesting the fragrant single-plant seed F6。
Planting hybrid A in Wenjiang of Sichuan in 20124F1Namely a high-quality fragrant three-line sterile line I6A with a planting fragrance F6Harvesting the fragrant seeds F7I.e., retention system I6B.
The I6A bred by the method is characterized in that the number of days for beginning sowing in the Wen river in Sichuan is about 58-60 days, the plant height is about 90cm, the main stem has 11 leaves, the plant type is compact, the stem is thin, the tillering force is moderate, 8-11 single plant ears are formed, the sword leaves are upright, the leaf color is deep, the leaf sheath is purple, the ear length is about 17-25 cm, the total grain number per ear is 130-160 grains, the grains are thin and long, the husk tips are purplish red, the grain length is 0.8-1.0 cm, the grain width is 0.25-0.30 cm, the length-width ratio is about 3.3-3.5, and the thousand seed weight is about 20-22 g, and the indexes can be different in different areas or different times due to the difference of natural environment and cultivation technology.
Analyzing the high-quality fragrant three-line sterile line I6A: I6A, wherein the brown rice rate is 79.8%, the polished rice rate is 70.2%, and the polished rice rate is 55.6%; appearance quality: chalkiness rate 9.7%, rice grain length 7.7mm, length-width ratio 3.4; the cooking quality is that the amylose content is 14%, the gelatinization temperature is 4.1, the consistency of the glue is 90mm, and the nutrition quality is as follows: the protein content was 8.1%.
While the foregoing description shows and describes several preferred embodiments of the invention, it is to be understood, as noted above, that the invention is not limited to the forms disclosed herein, but is not to be construed as excluding other embodiments and is capable of use in various other combinations, modifications, and environments and is capable of changes within the scope of the inventive concept as expressed herein, commensurate with the above teachings, or the skill or knowledge of the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (1)

1. A breeding method of a high-quality fragrant three-line sterile line I6A is characterized by comprising the following steps:
step 1, selecting D fragrance 1B containing fragrance type blood relationship as a female parent and gold 23B as a male parent to carry out artificial hybridization, and harvesting hybrid seeds;
step 2, planting hybrid F1Through 2 generations of systematic breeding, a single plant F with excellent rice quality, good resistance, excellent plant type, good comprehensive character and rice blast resistance is bred3The single plant is basically stable and contains fragrance;
step 3, using the gamboge 46A as a female parent, and hybridizing the maintainer line D, the incense 1B and the gold 23B3The fragrant later generation is used as a male parent to prepare a hybrid combination to obtain A1F1(ii) a Planting F3Fragrant single plant, harvesting fragrant single plant seed F4；
Step 4, in F4In the population, selection of individual plants F prone to the trait of interest4And with hit and select A1F1Hybridizing sterile single plants in pairs; preparing hybrid seedsA2F1(ii) a Planting F4Fragrant single plant, harvesting fragrant single plant seed F5；
Step 5, in F5In the population, selection of individual plants F prone to the trait of interest5And with hit and select A2F1The sterile single plants are hybridized in pairs to prepare hybrid A3F1(ii) a Planting F5Fragrant single plant, harvesting fragrant single plant seed F6；
Step 6, in F6In the population, selection of individual plants F prone to the trait of interest6And with hit and select A3F1The sterile single plants are hybridized in pairs to prepare hybrid A4F1Namely a high-quality fragrant three-line sterile line I6A with a planting fragrance F6Harvesting the fragrant single-plant seed F7I.e. the retention series I6B;
the single plant with the target character specifically refers to a single plant which has good plant type, more tillers, slender grain shape, more consistent properties of a plurality of single plants and fragrance;
the selection standard of the sterile single plants in the step 4 and the step 5 is as follows: the white thin anther is observed by naked eyes, and then the single plant with classical failure is determined as the sterile single plant.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1090165A (en) * 1993-10-28 1994-08-03 常德市农业科学研究所 Breeding method of high-quality hybrid rice sterile line gold 23A and maintainer line gold 23B thereof
CN1214856A (en) * 1998-12-03 1999-04-28 四川省农业科学院作物研究所 Breeding method of rice sterile line and its heterosis combination
CN107410003A (en) * 2017-05-04 2017-12-01 福建农林大学 A kind of high head rice rate and the special elongated Three-line rice sterile line breeding method of rice grain

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1090165A (en) * 1993-10-28 1994-08-03 常德市农业科学研究所 Breeding method of high-quality hybrid rice sterile line gold 23A and maintainer line gold 23B thereof
CN1214856A (en) * 1998-12-03 1999-04-28 四川省农业科学院作物研究所 Breeding method of rice sterile line and its heterosis combination
CN107410003A (en) * 2017-05-04 2017-12-01 福建农林大学 A kind of high head rice rate and the special elongated Three-line rice sterile line breeding method of rice grain

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
优质籼型三系不育系川华Ａ的选育与应用;龚桥等;《种子》;20181125;第37卷(第11期);第119页摘要，第120页图1 *
抗稻瘟病优质香稻不育系冈香1A的选育;周明镜等;《杂交水稻》;20130915;第28卷(第5期);第1节 选育经过 *

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