CN115287209B - Composite microbial agent and application thereof in treating swine waste water - Google Patents
Composite microbial agent and application thereof in treating swine waste water Download PDFInfo
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Abstract
The invention discloses a compound microbial agent and application thereof in treating swine waste water. The compound microbial agent consists of a Bacillus pumilus LZP strain, a Bacillus megaterium strain (Bacillus megaterium) LZP strain, a Bacillus subtilis strain (Bacillus subtilis) SC strain and a Bacillus bailii strain (Bacillus velezensis) WB strain. According to the invention, the composite rhizosphere growth promoting flora constructed by the four strains LZP, LZP02, WB and SC is used as a fermentation strain to ferment the pig raising wastewater, so that on one hand, the pollution sources such as ammonia nitrogen, organic matters, phosphorus and the like which are threatening to the environmental safety in the pig raising wastewater are reduced, and on the other hand, a large number of microbial thalli cultured by the pig raising wastewater can be used for collecting microbial preparations for preparing multiple aspects such as plant growth promotion, crop biocontrol, pollutant degradation and the like, thereby achieving two purposes.
Description
Technical Field
The invention relates to a compound microbial agent and application thereof in treating swine waste water, and also relates to a method for treating swine waste water. The invention belongs to the technical field of agricultural production.
Background
In recent years, livestock and poultry raising industry in China is greatly developed, and the production is gradually accelerated to the transition of large-scale, standardized, industrialized and intensified industries. The livestock and poultry raising industry which is vigorously developed provides rich food for people, and simultaneously, the livestock and poultry manure also causes pollution to the ecological environment.
Along with the continuous development of economy, the scale of the pig breeding industry chain is continuously enlarged, and the environmental pollution problem is also more and more prominent. Most live pig farms are cleaned by flushing water, so that pig urine, excrement and the like in pig farm waste water are mixed with each other, the concentration of ammonia nitrogen, phosphorus, suspended matters and organic matters in the waste water is high, the carbon nitrogen ratio is seriously unbalanced, and if a large amount of untreated discharge is carried out on environments such as lakes, rivers, farmlands and the like, the water body is enriched, the soil property is changed and other column pollution problems are caused.
At present, the pig raising wastewater treatment technology at home and abroad mainly focuses on three major modes, namely a physical and chemical treatment technology, a natural treatment technology and a biological treatment technology. Among the methods used in the physicochemical treatment are a medium adsorption method, a flocculation precipitation method and the like, such as Qian Feng, the zeolite-straw combination is used for filtering the swine waste water, and the removal rates of COD, ammoniacal nitrogen and phosphorus can reach 47.9%, 72.9% and 50.1% (Qian Feng, 2008) respectively, and 61.02% of swine waste water with the COD concentration of 3232mg/L can be removed by a magnetic flocculation method like Cui Lina and the like (Cui Lina, 2010). Although the physicochemical method has better pollutant removal rate on the swine waste water, the physicochemical method has the disadvantages of lower broad spectrum, higher pollutant removal cost, larger investment of treatment equipment, less engineering application and the like on the swine waste water pollution removal. The natural treatment method generally utilizes the comprehensive action of natural soil, water body and organisms to remove pollutants, such as Zhu Xizhen, and the like, and the artificial wetland construction is carried out through blast furnace slag, quartz sand and other matrixes, so that the removal rates of COD, BOD and phosphorus respectively reach 71% -88%, 80% -89%, 70% -85% (Zhu Xizhen, 2003), and Lu Xiuguo and the like, and the pig raising wastewater COD is less than or equal to 400mg/L and ammonia nitrogen is less than or equal to 70mg/L (Lu Xiuguo, 2009) by utilizing the oxidation pond. The natural treatment method has better decontamination capability on polluted wastewater, but has insufficient stability and longer purification time. The biological treatment technology is a novel method for treating high-concentration organic wastewater by using the catalysis of microorganisms, such as Liang Meidong, and the like, and the aeration treatment of pig raising wastewater by using an SRB reactor can increase the removal rate of COD to more than 90% (Liang Meidong, 2009), and Li Fengmin and the like can enable the removal rate of ammonia nitrogen and total nitrogen to reach 99.7% and 50.7% by adopting an aerobic-anaerobic combined treatment method (Li Fengmin, 2011). However, a large amount of activated sludge is generated in the process of treating the wastewater by a biological treatment method, and cannot be treated, so that secondary pollution is caused to the environment.
Although the pig raising waste water causes huge pressure on the environment, the pig raising waste water contains a great amount of organic matters, nitrogen, phosphorus, potassium and other nutrient elements. In the first Chinese public inspection notice of pollution in 2010, it is clearly pointed out that total nitrogen and total phosphorus are the foremost pollutant emissions of the livestock and poultry raising industry, the emissions are 102.48 ten thousand tons and 16.04 ten thousand tons respectively, and the total nitrogen and total phosphorus emissions in the national water pollution respectively account for 21.7% and 37.9%. Therefore, the feces and the wastewater generated by livestock are very important resources, and the potential of recycling is huge. If the pig raising waste water is used as a basic culture medium for fermentation production of beneficial microorganisms, the production cost of the microbial fertilizer can be greatly reduced, and the pollution problem can be reduced through biodegradation, so that the waste water can be changed into valuable, and the residual value of the waste water is realized.
According to the invention, the composite rhizosphere growth promoting flora constructed by the four strains LZP, LZP02, WB and SC is used as a fermentation strain to ferment the pig raising wastewater, so that on one hand, the pollution sources such as ammonia nitrogen, organic matters, phosphorus and the like which are threatening to the environmental safety in the pig raising wastewater are reduced, and on the other hand, a large number of microbial thalli cultured by the pig raising wastewater can be used for collecting microbial preparations for preparing multiple aspects such as plant growth promotion, crop biocontrol, pollutant degradation and the like, thereby achieving two purposes.
Disclosure of Invention
The invention aims at providing a compound microbial agent for treating pig raising wastewater;
the second object of the invention is to provide a method for treating pig raising wastewater by using the composite microbial agent.
In order to achieve the above purpose, the invention adopts the following technical means:
the invention discloses a composite microbial agent, which consists of a Bacillus pumilus LZP strain, a Bacillus megaterium (Bacillus megaterium) LZP strain, a Bacillus subtilis (Bacillus subtilis) SC strain and a Bacillus bailii (Bacillus velezensis) WB strain, wherein the Bacillus pumilus LZP strain is preserved in China center for type culture collection, the strain preservation number of the Bacillus pumilus 2018598 is CCTCC NO. M2018598, the Bacillus megaterium LZP strain 3703 is preserved in China center for type culture collection, the strain preservation number of the Bacillus megaterium is CCTCC NO. M2018599, and the Bacillus subtilis (Bacillus subtilis) SC strain is preserved in the China center for type culture collection, and the strain preservation number of the Bacillus bailii is CCTCC NO: m20211546, the Bacillus belicus (Bacillus velezensis) WB strain is preserved in China center for type culture collection, and has a strain preservation number of CCTCC NO: m20211547.
Furthermore, the invention also provides application of the composite microbial agent in treating pig raising wastewater.
Preferably, seed solutions of the Bacillus pumilus LZP strain, the Bacillus megaterium strain (Bacillus megaterium) LZP strain, the Bacillus subtilis strain (Bacillus subtilis) SC strain and the Bacillus bailii strain (Bacillus velezensis) WB strain are respectively inoculated into sterilized pig raising wastewater to be treated, and after fermentation, the treated pig raising wastewater is obtained, and compared with the treated pig raising wastewater, the treated pig raising wastewater has reduced pollutant content.
Wherein, preferably, the application further comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
preferably, the seed solution of Bacillus pumilus LZP strain, bacillus megaterium strain (Bacillus megaterium) LZP strain, bacillus subtilis strain (Bacillus subtilis) SC strain and Bacillus bailii strain (Bacillus velezensis) WB strain is prepared according to a volume ratio of 1:1:1:1, after mixing, inoculating the mixture into sterilized pig raising wastewater to be treated; the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
Preferably, the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus in the treated pig raising wastewater is reduced compared with the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus before treatment.
Furthermore, the invention also provides a method for treating pig raising wastewater, which comprises the following steps:
(1) Inoculating Bacillus pumilus LZP, bacillus megaterium (Bacillus megaterium) LZP, bacillus subtilis (Bacillus subtilis) SC and Bacillus berryis (Bacillus velezensis) WB according to the invention into beef extract peptone solid culture medium respectively for activation, multiplexing activated strain WB into beef extract peptone liquid culture medium, and regulating strain concentration to OD by using sterile water 600 =1.0 as seed fluid;
(2) Mixing LZP, LZP, WB and SC activated bacteria liquid according to a volume ratio of 1:1:1, inoculating the mixed bacteria liquid into sterilized pig raising wastewater to be treated, and fermenting to obtain treated pig raising wastewater, wherein the treated pig raising wastewater has reduced pollutant content compared with the treated pig raising wastewater.
Wherein, preferably, the step (2) further comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
preferably, in the step (2), the seed solution of Bacillus pumilus LZP strain, bacillus megaterium (Bacillus megaterium) LZP strain, bacillus subtilis (Bacillus subtilis) SC strain and Bacillus bailii (Bacillus velezensis) WB strain is prepared according to a volume ratio of 1:1:1:1, inoculating the mixture into sterilized pig raising wastewater to be treated according to an inoculum size of 1-2 vol%; the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
Preferably, the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus in the treated pig wastewater is reduced compared with the total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus before treatment.
Compared with the prior art, the invention has the beneficial effects that:
the invention takes a composite microbial agent obtained by constructing four strains of Bacillus pumilus LZP strain, bacillus megaterium (Bacillus megaterium) LZP strain, bacillus subtilis (Bacillus subtilis) SC strain and Bacillus belicus (Bacillus velezensis) WB strain as fermentation strains, and proves that LZP (Bacillus megaterium) and LZP (Bacillus pumilus) are taken as a rice rhizosphere growth promoting strain to effectively promote the growth and development of rice seedlings and improve the nutrient absorption capacity of the rice seedlings, WB (Bacillus velezensis) can obviously inhibit the growth of crop pathogenic bacteria fusarium oxysporum, improve the disease resistance of crops and promote the yield increase of crops, and SC (Bacillus subtilis) has good capability of preventing and treating watermelon fusarium wilt, so that the bacterial strain is adopted to inoculate in pig raising wastewater for fermentation to reduce pollutants in the pig raising wastewater. Compared with the traditional method, the method has the advantages that the method can better resist high COD, reduce pH, reduce ammonia nitrogen, phosphorus, total nitrogen, total organic matters and the like, can obtain beneficial microorganisms for producing microbial fertilizers through filtration after fermentation is finished, can obtain economic benefits while reducing pollutant cost, and can enable the compound bacteria to rapidly grow in the pig-raising wastewater through adjusting the carbon-nitrogen ratio of the pig-raising wastewater, so that the pig-raising wastewater treatment period is shortened, and a beneficial condition is created for continuously carrying out a large amount of pollutant treatment.
In addition, the single-strain fermented microbial fertilizer is used, so that the conditions of single effect, poor adaptability and the like of some products possibly occur, the single-strain fermented microbial fertilizer cannot play a role in the plant growth and development process, the functionality of the single-strain fermented microbial fertilizer is weakened, antagonism among strains is found through a flat-plate counter test by LZP, LZP, WB and SC strains, and the single-strain fermented microbial fertilizer has different functions, so that the defect of single-strain fermented microbial fertilizer can be effectively avoided, the adaptability of the fertilizer in soil is improved, the maximum release of fertilizer efficiency is ensured, sufficient nutrition is provided for crops in multiple directions, and a suitable growth environment is ensured, so that the produced composite microbial fertilizer has stronger functions and more various functions in the broad-spectrum application and can be better applied to agricultural yield increase. Compared with single strain for pig raising waste water fermentation, the method has the advantages that the growth speed of LZP03-LZP02-WB-SC composite bacteria in pig urine waste water is faster, the maximum biomass can be achieved within 48 hours, the fermentation period is greatly shortened, the yield is improved, and meanwhile, the method has a certain removal effect on pollutants such as COD, ammoniacal nitrogen and organic matters in pig raising waste water.
Drawings
FIG. 1 shows the biomass change with time in swine wastewater after optimization of single and composite bacterial agents;
wherein A: LZP02; b: LZP03; c: SC; d: WB; e: LZP 03-LZP-WB-SC complex bacteria;
FIG. 2 is a graph showing the trend of change and the decrease rate of total nitrogen, total carbon and total organic carbon in the supernatant of the composite microbial inoculum;
FIG. 3 shows the pH variation trend of the composite microbial inoculum in an optimized culture medium along with the fermentation time;
FIG. 4 shows the trend of removal of COD in the swine wastewater over time in the complex microbial agent fermentation broth;
FIG. 5 shows the removal rate of ammonia nitrogen and phosphorus content in the pig wastewater by the composite microbial inoculum.
Strain preservation information:
the Bacillus pumilus LZP02 is named Bacillus pumilus LZP02, is classified and named Bacillus pumilus LZP02 respectively, is preserved in China center for type culture collection, and has a strain preservation number of CCTCC No. M2018598 at university of Wuhan, china, and has a preservation time of 2018, 9 and 6 days.
The bacillus megatherium LZP03 is named Bacillus megaterium LZP03, is classified and named Bacillus megaterium LZP03 respectively, is preserved in China center for type culture Collection, and has a strain preservation number of CCTCC No. M2018599 and a preservation time of 2018, 9 and 6 days at university of Wuhan and Wuhan.
The bacillus subtilis strain SC is named Bacillus subtilis SC, classified and named Bacillus subtilis SC, is preserved in China center for type culture collection, and has a strain preservation number of CCTCC NO: m20211546, storage time is 2021, 12 and 6.
The bacillus belicus strain WB is named Bacillus velezensis WB, classified and named Bacillus velezensis WB, and is preserved in China center for type culture collection, and the address is in university of Wuhan, china, and the strain preservation number is CCTCC NO: m20211547, storage time is 2021, 12 and 6.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in connection with the embodiments of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present invention are within the protection scope of the present invention.
Example 1 growth of Single microbial agent and Compound microbial agent in Swine urine wastewater
1 materials and methods
1.1 test Strain Source
The strains used in the test are all object-separated and screened different kinds of pure culture beneficial microorganisms, and researches find that each strain is a high-efficiency strain with better industrial application potential in the aspects of plant growth promotion, biological control, pollutant control and the like, each strain can rapidly grow in pig raising wastewater and has better removal effect on ammonia nitrogen, phosphorus, organic matters and the like, the preservation place is a refrigerator at the temperature of minus 80 ℃, and all strains are identified by classification of strain morphology and 16SrDNA, and an identification list is shown in a table 1.
TABLE 1 test strains
1.2 physical and chemical Properties of pig raising wastewater
The swine waste water used in the test was taken from a swine farm in zizihaar Fula base region, and the basic characteristics of the collected swine waste water are shown in table 2.
TABLE 2 physicochemical Properties of raw pig raising wastewater
1.3 Medium
Beef extract peptone medium: 10.00g of peptone, 3.00g of beef extract, 5.00g of NaCl, 20.00g of agar, and distilled water with a constant volume of 1000.00mL, wherein agar is not added into the liquid culture medium.
Pig raising wastewater culture medium with optimized carbon-nitrogen ratio: 10g of brown sugar and the volume of the pig raising wastewater is fixed to 1000.00mL.
1.4 research methods
(1) Single strain fermentation culture
The beneficial microorganism strains LZP, LZP02, WB and SC preserved at-80 ℃ are respectively inoculated into a beef extract peptone solid culture medium and are cultured for 24 hours at 30 ℃ for activation. Multiplexing the strains after 2-3 loops activation in a 250mL triangular flask filled with 100mL beef extract peptone liquid medium under aseptic operation conditions, carrying out shaking culture at 30 ℃ and 120r/min, taking each strain cultured to the same concentration (OD 600 = 1.0) as seed liquid, inoculating the activated bacterial liquids of LZP03, LZP02, WB and SC into 300mL pig raising wastewater medium according to the inoculum size of 1vol%, carrying out shaking culture at 30 ℃ and 120r/min, sampling samples every 24h, and measuring the bacterial growth of a single bacterial agent in the optimized pig raising wastewater medium along with time;
(2) Composite strain fermentation culture
The beneficial microorganism strains LZP, LZP02, WB and SC preserved at-80 ℃ are respectively inoculated into a beef extract peptone solid culture medium and are cultured for 24 hours at 30 ℃ for activation. The method comprises the steps of multiplexing 2-3 loops of activated strains into a 250mL triangular flask filled with 100mL of beef extract peptone liquid medium under aseptic operation conditions, performing shaking culture at 30 ℃ and 120r/min, taking each strain cultured to the same concentration (OD 600 = 1.0) as seed liquid, mixing LZP, LZP02, WB and SC activated bacteria liquid according to the volume ratio of 1:1:1:1, inoculating the activated bacteria liquid into 300mL of pig raising waste water medium according to the inoculum size of 1vol%, culturing at 30 ℃ and 120r/min in a shaking flask, sampling samples every 24h, and determining the growth of single microbial agents or composite microbial agents in the optimized pig raising waste water medium along with time.
2. Results
In order to determine the change condition of biomass of the single microbial agent and the compound microbial agent in the culture medium along with time, the activated strain is inoculated in the optimized culture medium, and the living bacterial count is measured by a gradient dilution plating method.
As can be seen from FIG. 1A, LZP02 was cultured in an optimized pig raising waste water medium for 72 hours to a maximum viable count of 4.07×10 10 cfu/mL. As can be seen from FIG. 1B, the maximum viable count of 4.26X10 s is reached in LZP03 in the optimized pig raising wastewater medium for 96 hours 10 cfu/mL. From the graph1C shows that the SC strain is cultured in the pig raising wastewater culture medium with optimized carbon-nitrogen ratio for 48 hours, and the maximum viable count is 4.16X10 10 cfu/mL viable count. As can be seen from FIG. 1D, the maximum biomass of the WB strain in the original medium after 48 hours was 4.64×10 10 cfu/mL. As can be seen from FIG. 1E, the growth of the composite microbial inoculum in the pig urine culture medium shows a trend of increasing and then decreasing and tending to stabilize with time, and the number of viable bacteria reaches a maximum biomass of 3.84×10 after 48 hours of culture 10 cfu/mL, the subsequent biomass reduction may be due to the fact that brown sugar as a quick-acting carbon source can be rapidly utilized by microorganisms but as the utilization of growth increases, the carbon nitrogen ratio changes to affect the growth of microorganisms, resulting in a decrease in microbial biomass.
Compared with the fermentation of the pig raising wastewater by a single strain, the method has the advantages that the growth speed of the LZP03-LZP02-WB-SC composite strain in the pig urine wastewater is faster, the maximum biomass can be achieved within 48 hours, the fermentation period is greatly shortened, and the output is improved.
Example 2 Compound microbial inoculant and application thereof in treatment of swine wastewater
1 materials and methods
1.1 test Strain Source
As in example 1.
1.2 physical and chemical Properties of pig raising wastewater
The swine waste water used in the test is taken from a swine farm in the zizihaar Fula base region, 1wt% brown sugar is added into the collected swine waste water to optimize the carbon nitrogen ratio as a basic fermentation medium, and the basic characteristics of the optimized swine waste water are shown in table 3.
TABLE 3 physicochemical Properties of the waste Water from pig raising after optimization
1.3 Medium
Beef extract peptone medium: 10.00g of peptone, 3.00g of beef extract, 5.00g of NaCl, 20.00g of agar, and distilled water with a constant volume of 1000.00mL, wherein agar is not added into the liquid culture medium.
1.4 research methods
The beneficial microorganism strains LZP, LZP02, WB and SC preserved at-80 ℃ are respectively inoculated into a beef extract peptone solid culture medium and are cultured for 24 hours at 30 ℃ for activation. The 2-3 loop activated strain is multiplexed in a 250mL triangular flask filled with 100mL beef extract peptone liquid medium under aseptic operation conditions by using an inoculating loop, shaking culture is carried out at 30 ℃ and 120r/min, then each strain cultured to the same concentration (OD 600 = 1.0) is used as seed liquid, then LZP, LZP02, WB and SC activated bacterial liquid is mixed according to the volume ratio of 1:1:1:1, the mixture is inoculated in 300mL pig raising waste water medium according to the inoculum size of 1vol%, then shaking flask culture is carried out at 30 ℃ and 120r/min, samples are sampled every 24h, centrifugation is carried out for 5min under the condition of 12000rpm, and index measurement is carried out.
1.5 test measurement index
1.5.1 Water quality index
And measuring the pH, total nitrogen, total carbon, total organic carbon, ammonia nitrogen and phosphorus content of the sample.
1.5.2 microbial indicators
10mL of fermentation broth is put into a shaking table with 90mL of sterile water at 200r/min to shake for 30min, and then gradient dilution is carried out to obtain 10 -7 、10 -8 、10 -9 And (3) taking 0.1mL of the diluted solution, coating the diluted solution on a beef extract peptone culture medium plate, culturing at 30 ℃ for 24 hours, and calculating the viable count (cfu/mL) to form three groups of parallel groups.
2. Results and discussion
2.1 Change of various indexes of fermentation waste liquid after removing thalli from pig raising waste water treated by composite microbial agent
2.1.1 variation of total Nitrogen, total carbon and Total organic carbon content in optimized pig raising wastewater by composite microbial inoculant
Inoculating the composite microbial agent into an optimized culture medium, and measuring total nitrogen of the fermentation broth every 24 hours. As can be seen from FIG. 2, after the composite microbial agent is inoculated in the optimized culture medium, the total nitrogen content of the fermentation liquid shows a general decline trend along with the extension of the fermentation time, and the total nitrogen content is rapidly decreased in the fermentation interval of 0-24h and then gradually flattened, and the minimum value of the total nitrogen content reaches 102.2mg/L when the culture is carried out for 96h, and compared with the control group, the total nitrogen content is decreased by 73.55%. There is also a significant decrease in the change in total carbon of the composite microbial agent as shown in figure 2. The total carbon content in the fermentation liquor shows a slow descending trend along with the increase of the culture time, the minimum value of the total carbon content of the fermentation liquor of the composite microbial agent is 3010mg/L, compared with the control group, the total carbon descending rate of the fermentation liquor of the composite microbial agent after 96 hours of culture is 48.55 percent, and the reduction of the total carbon content is probably due to the fact that the total carbon content is reduced due to the fact that microorganisms continuously breathe in the growth process to discharge carbon dioxide, so that the microorganisms can grow and reproduce actively in the pig raising wastewater. In terms of total organic carbon, the composite microbial agent is inoculated into an optimized pig raising wastewater culture medium, the total organic carbon content of the fermentation liquid continuously decreases along with the extension of the culture time and finally tends to be stable, the total organic carbon content of the centrifugal fermentation liquid rapidly decreases to reach the minimum value in 48h and then tends to be stable after slightly rising in 72h in a fermentation time period of 0-24h, and compared with a control group, the minimum organic carbon decrease rate of the composite microbial agent reaches 54.10%.
2.1.2 pH variation of the composite microbial inoculant in the optimized pig raising wastewater
The pH measurement is carried out on the fermentation liquor of the composite microbial agent every 24 hours, and as can be known from fig. 3, after the pig raising wastewater is fermented for 96 hours by the composite microbial agent, the pH of the pig raising wastewater is reduced from the original weak alkaline solution with the pH of 7.82 to the weak acidic fermentation liquor with the pH of 6.51, which is probably caused by the reduction of the pH value due to the secretion of organic acid by the thalli, and the method has important significance for preventing the threat of the pig raising wastewater to soil salinization.
2.1.3 COD content variation and removal rate of the composite microbial inoculant in the optimized pig raising wastewater
In order to determine the COD removal effect of the composite microbial inoculum on the pig raising wastewater, the COD detection is carried out on the supernatant of the fermentation liquid, and as the fermentation time of the strain is continuously increased, the COD content of the strain is continuously and rapidly reduced from the original COD=27400 mg/L to the maximum COD=9360 mg/L, and the COD removal rate reaches 65.38%, which indicates that the composite microbial inoculum has a certain removal rate on the pig raising wastewater COD and reduces the threat of filtered waste liquid to the environment.
2.1.4 removal rate of ammonia nitrogen and phosphorus in the optimized pig raising wastewater by the composite microbial agent
The ammonia nitrogen content of the supernatant fluid of the fermentation liquid of the composite microbial agent at different fermentation times is measured, and the figure 5 shows that the composite microbial agent can greatly reduce the ammonia nitrogen content in the fermentation waste liquid, the ammonia nitrogen removal rate reaches 58.17%, and the ammonia nitrogen removal effect is relatively good. After the fermentation, the content of phosphorus in the supernatant fluid of the fermentation liquid is measured, so that whether the strain can well reduce the content removal rate of phosphorus in the fermentation liquid waste can reach 44.77 percent is judged, and the strain has good phosphorus removal capability.
Claims (10)
1. The composite microbial agent is characterized by comprising a Bacillus pumilus (Bacillus pumilus) LZP strain, a Bacillus megaterium (Bacillus megaterium) LZP strain, a Bacillus subtilis (Bacillus subtilis) SC strain and a Bacillus bailii (Bacillus velezensis) WB strain, wherein the Bacillus pumilus LZP strain is preserved in China center for type culture collection, the strain preservation number of the Bacillus pumilus 2018598 is CCTCC NO. M2018598, the Bacillus megaterium LZP strain is preserved in China center for type culture collection, the strain preservation number of the Bacillus pumilus is CCTCC NO. M2018599, and the Bacillus subtilis (Bacillus subtilis) SC strain is preserved in China center for type culture collection, and the strain preservation number of the Bacillus pumilus is CCTCC NO: m20211546, the Bacillus belicus (Bacillus velezensis) WB strain is preserved in China center for type culture collection, and has a strain preservation number of CCTCC NO: m20211547.
2. The use of the composite microbial agent of claim 1 in treating swine wastewater.
3. The use according to claim 2, wherein the seed liquids of bacillus pumilus LZP, bacillus megaterium (Bacillus megaterium) LZP, bacillus subtilis (Bacillus subtilis) SC and bacillus bailii (Bacillus velezensis) WB are respectively inoculated into sterilized swine wastewater to be treated, and the post-treatment swine wastewater is obtained after fermentation, wherein the post-treatment swine wastewater has a reduced content of contaminants compared to the pre-treatment swine wastewater.
4. The use according to claim 3, further comprising the step of adding brown sugar to the sterilized swine waste water to be treated such that the swine waste water has a carbon to nitrogen ratio of 16-20:1.
5. the use as claimed in claim 3, wherein the seed solution of Bacillus pumilus (Bacillus pumilus) LZP strain, bacillus megaterium (Bacillus megaterium) LZP strain, bacillus subtilis (Bacillus subtilis) SC strain and Bacillus bailii (Bacillus velezensis) WB strain is prepared by the following steps: 1:1:1, after mixing, inoculating the mixture into sterilized pig raising wastewater to be treated; the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
6. The use according to claim 3, wherein the treated swine wastewater has reduced levels of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus as compared to the pre-treatment swine wastewater.
7. A method for treating pig raising wastewater, comprising the steps of:
(1) Inoculating Bacillus pumilus LZP strain, bacillus megaterium strain (Bacillus megaterium) LZP strain, bacillus subtilis strain (Bacillus subtilis) SC strain and Bacillus bailii strain (Bacillus velezensis) WB strain described in claim 1 into beef extract peptone solid culture medium respectively for activation, multiplexing activated strain WB into beef extract peptone liquid culture medium, and regulating strain concentration to OD with sterile water 600 =1.0 as seed fluid;
(2) Mixing LZP, LZP, WB and SC activated bacteria liquid according to a volume ratio of 1:1:1, inoculating the mixed bacteria liquid into sterilized pig raising wastewater to be treated, and fermenting to obtain treated pig raising wastewater, wherein the treated pig raising wastewater has reduced pollutant content compared with the treated pig raising wastewater.
8. The method of claim 7, wherein the step (2) further comprises the step of adding brown sugar to the sterilized swine waste water to be treated such that the swine waste water has a carbon to nitrogen ratio of 16-20:1.
9. the method according to claim 7, wherein in the step (2), the seed solution of Bacillus pumilus LZP strain, bacillus megaterium strain (Bacillus megaterium) LZP strain, bacillus subtilis strain (Bacillus subtilis) SC strain and Bacillus bailii strain (Bacillus velezensis) WB strain is prepared in a volume ratio of 1:1:1:1, inoculating the mixture into sterilized pig raising wastewater to be treated according to an inoculum size of 1-2 vol%; the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
10. The method of claim 7, wherein the treated swine wastewater has reduced levels of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus, and pH as compared to the pre-treatment swine wastewater.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107964514A (en) * | 2017-09-08 | 2018-04-27 | 华夏春秋科技发展（北京）有限责任公司 | A kind of Bei Laisi bacillus and its application on plant |
| CN110312694A (en) * | 2017-01-12 | 2019-10-08 | 喜施倍全球股份有限公司 | System and method for water remediation |
| CN115353211A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Application of bacillus megaterium LZP03 in treatment of pig raising wastewater |
| CN115353987A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Bacillus subtilis strain SC strain for treating pig raising wastewater and application thereof |
| CN115353210A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Application of bacillus pumilus LZP02 in treatment of pig raising wastewater |
| CN115353986A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Bacillus belgii strain WB strain for treating swine wastewater and application thereof |
-
2022
- 2022-01-28 CN CN202210112144.0A patent/CN115287209B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110312694A (en) * | 2017-01-12 | 2019-10-08 | 喜施倍全球股份有限公司 | System and method for water remediation |
| CN107964514A (en) * | 2017-09-08 | 2018-04-27 | 华夏春秋科技发展（北京）有限责任公司 | A kind of Bei Laisi bacillus and its application on plant |
| CN115353211A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Application of bacillus megaterium LZP03 in treatment of pig raising wastewater |
| CN115353987A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Bacillus subtilis strain SC strain for treating pig raising wastewater and application thereof |
| CN115353210A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Application of bacillus pumilus LZP02 in treatment of pig raising wastewater |
| CN115353986A (en) * | 2022-01-28 | 2022-11-18 | 齐齐哈尔大学 | Bacillus belgii strain WB strain for treating swine wastewater and application thereof |
Non-Patent Citations (6)
| Title |
|---|
| Bacillus pumilus LZP02 Promotes Rice Root Growth by Improving Carbohydrate Metabolism and Phenylpropanoid Biosynthesis;Hong Liu等;Molecular Plant-Microbe Iteractions;第33卷(第10期);全文 * |
| 新型芽孢杆菌在农业领域应用研究进展;袁海峰;周舒扬;甄涛;赵晓宇;;国土与自然资源研究(02);全文 * |
| 根际芽孢杆菌对水稻根系的促生效应;徐伟慧等;河南农业科学;第47卷(第4期);全文 * |
| 水稻根际促生菌的筛选鉴定及促生能力分析;刘泽平等;农业资源与环境学报;35(2);全文 * |
| 芽孢杆菌特性及其在畜牧生态养殖中应用研究进展;曹树威等;中国兽药杂志;第55卷(第11期);全文 * |
| 贝莱斯芽孢杆菌E69预防稻瘟病等多种真菌病害的潜力;沙月霞;隋书婷;曾庆超;沈瑞清;;中国农业科学(11);全文 * |
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