CN115287209A - Compound microbial agent and application thereof in treatment of swine urine wastewater - Google Patents
Compound microbial agent and application thereof in treatment of swine urine wastewater Download PDFInfo
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Abstract
The invention discloses a compound microbial agent and application thereof in treatment of swine urine wastewater. The compound microbial agent consists of a Bacillus pumilus (Bacillus pumilus) LZP02 strain, a Bacillus megaterium (Bacillus megaterium) LZP03 strain, a Bacillus subtilis (Bacillus subtilis) SC strain and a Bacillus velezensis (Bacillus velezensis) WB strain. According to the invention, the composite rhizosphere growth-promoting flora constructed by the four strains LZP03, LZP02, WB and SC is used as a fermentation strain to ferment the pig raising wastewater, so that pollution sources such as ammonia nitrogen, organic matters, phosphorus and the like which threaten the environmental safety in the pig raising wastewater are reduced, and a large amount of microbial thalli cultured by the pig raising wastewater can be collected to prepare microbial preparations in various aspects such as plant growth promotion, crop biocontrol, pollutant degradation and the like, thereby achieving two purposes.
Description
Technical Field
The invention relates to a compound microbial agent and application thereof in treatment of swine urine wastewater, and also relates to a method for treating swine wastewater. The invention belongs to the technical field of agricultural production.
Background
In recent years, livestock and poultry breeding in our country has been greatly developed, and the production is gradually accelerated towards scale, standardization, industrialization and intensification. The booming livestock and poultry breeding industry provides abundant food for people, and meanwhile, the livestock and poultry manure causes pollution to the ecological environment.
Along with the continuous development of economy, the scale of the pig breeding industry chain is continuously enlarged, and the problem of environmental pollution caused by the pig breeding industry chain is more and more prominent. As most pig farms clean pig houses by flushing water, pig urine and excrement in pig farm wastewater are mixed with each other, the ammonia nitrogen content, the phosphorus content, suspended matters and organic matter concentration in the wastewater are high, the carbon nitrogen ratio is seriously unbalanced, and if a large amount of wastewater is discharged without treatment, the wastewater causes pollution problems such as water eutrophication, soil property change and the like in lakes, rivers, farmlands and other environments.
At present, the pig-raising wastewater treatment technology at home and abroad mainly focuses on three main types of modes, namely a physicochemical treatment technology, a natural treatment technology and a biological treatment technology. The physical and chemical treatment methods are mostly used, such as a medium adsorption method, a flocculation precipitation method and the like, for example, qianfeng and the like are used for filtering the swine urine wastewater by utilizing a zeolite-straw combination, the removal rates of COD, ammoniacal nitrogen and phosphorus can respectively reach 47.9%, 72.9% and 50.1% (Qianfeng, 2008), and like Traine and the like, 61.02% (Traine, 2010) of the swine wastewater with the COD concentration of 3232mg/L can be removed by a magnetic flocculation method. Although the physicochemical method has better pollutant removal rate on the pig urine wastewater, the physicochemical method has the disadvantages of lower broad spectrum for removing the pig urine wastewater pollution, higher pollutant removal cost, larger investment on treatment equipment, less engineering application and the like. The natural treatment method is generally to remove pollutants by utilizing the comprehensive utilization of natural soil, water and organisms, such as Zhu Xiezhen and the like, the removal rates of COD, BOD and phosphorus respectively reach 71-88%, 80-89% and 70-85% (Zhu Xiezhen, 2003) by carrying out artificial wetland construction through substrates such as blast furnace slag and quartz sand, and the COD of pig-raising wastewater treated by an oxidation pond of Luxiu and the like is less than or equal to 400mg/L and the ammonia nitrogen is less than or equal to 70mg/L (Luxiu, 2009). The natural treatment method has good decontamination capability on polluted wastewater, but has insufficient stability and long purification time. The biological treatment technology is a novel method for treating high-concentration organic wastewater by using the catalytic action of microorganisms, such as that pig raising wastewater subjected to aeration treatment by using an SRB reactor in Shandong et al can increase the removal rate of COD to over 90% (Shandong, 2009), and Lifeng et al can increase the removal rates of ammonia nitrogen and total nitrogen to 99.7% and 50.7% (Lifeng, 2011) by using an aerobic-anaerobic combined treatment method. However, in the process of treating wastewater by biological treatment, a large amount of activated sludge is generated and cannot be treated, so that secondary pollution is caused to the environment.
Although pig raising wastewater exerts great pressure on the environment, the pig raising wastewater contains a large amount of organic matters and nutrient elements such as nitrogen, phosphorus, potassium and the like. In the first Chinese pollution census announcement in 2010, it is clearly indicated that total nitrogen and total phosphorus are the most important pollutant emissions of the livestock and poultry breeding industry, the emission amounts of the total nitrogen and the total phosphorus are 102.48 ten thousand tons and 16.04 ten thousand tons respectively, and the emission amounts of the total nitrogen and the total phosphorus in the national water pollution account for 21.7 percent and 37.9 percent respectively. Therefore, the excrement and the waste water generated by the livestock are very important resources, and the potential of resource utilization is huge. If the pig-raising wastewater is used as a basic culture medium for beneficial microbial fermentation production, the production cost of the microbial fertilizer can be greatly reduced, and the pollution problem can be reduced through biodegradation, so that the wastewater can be changed into valuable, and the residual value of the wastewater is realized.
According to the invention, the composite rhizosphere growth-promoting flora constructed by the four strains LZP03, LZP02, WB and SC is used as a fermentation strain to ferment the pig raising wastewater, so that pollution sources such as ammoniacal nitrogen, organic matters, phosphorus and the like which threaten the environmental safety in the pig raising wastewater are reduced, and a large amount of microbial thalli cultured by the pig raising wastewater can be collected to prepare microbial preparations in various aspects such as plant growth promotion, crop biocontrol, pollutant degradation and the like, thereby achieving two purposes at one stroke.
Disclosure of Invention
One of the purposes of the invention is to provide a compound microbial agent for treating pig-raising wastewater;
the second purpose of the invention is to provide a method for treating swine wastewater by using the compound microbial agent.
In order to achieve the purpose, the invention adopts the following technical means:
the invention relates to a compound microbial agent, which consists of a Bacillus pumilus (Bacillus pumilus) LZP02 strain, a Bacillus megaterium (Bacillus megaterium) LZP03 strain, a Bacillus subtilis (Bacillus subtilis) SC strain and a Bacillus velezensis (Bacillus velezensis) WB strain, wherein the Bacillus pumilus LZP02 strain is preserved in a China center for type culture collection, the strain preservation number is CCTCC NO.M 2018598, the Bacillus megaterium LZP03 strain is preserved in a China center for type culture collection, the strain preservation number is CCTCC NO.M 2018599, the Bacillus subtilis (Bacillus subtilis) SC strain is preserved in a China center for type culture collection, the strain preservation number is CCTCC NO: m20211546, wherein the Bacillus velezensis WB strain is preserved in China center for type culture Collection, and the strain preservation number is CCTCC NO: m20211547.
Furthermore, the invention also provides application of the compound microbial agent in treatment of pig raising wastewater.
Preferably, seed liquids of the Bacillus pumilus (LZP 02 strain, the Bacillus megaterium (LZP 03 strain), the Bacillus Subtilis (SC) strain and the Bacillus belius (WB) strain are respectively inoculated into the sterilized to-be-treated pig raising wastewater, the treated pig raising wastewater can be obtained through fermentation, and the content of pollutants in the treated pig raising wastewater is reduced compared with that in the treated pig raising wastewater.
Preferably, the application further comprises the step of adding brown sugar into the sterilized swine wastewater to be treated, so that the carbon-nitrogen ratio of the swine wastewater is 16-20:1.
wherein, preferably, the seed liquid of the Bacillus pumilus (Bacillus pumilus) LZP02 strain, the Bacillus megaterium (Bacillus megaterium) LZP03 strain, the Bacillus subtilis (Bacillus subtilis) SC strain and the Bacillus beleisis (Bacillus velezensis) WB strain is mixed according to the volume ratio of 1:1:1:1, mixing, and inoculating the mixture into sterilized wastewater to be treated for pig raising; the fermentation refers to fermentation culture at 30 ℃ and 120r/min for 24-96h.
Preferably, the pig raising wastewater after treatment has reduced contents of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus compared with the pig raising wastewater before treatment.
Furthermore, the invention also provides a method for treating the pig raising wastewater, which comprises the following steps:
(1) Respectively inoculating the Bacillus pumilus (Bacillus pumilus) LZP02 strain, the Bacillus megaterium (Bacillus megaterium) LZP03 strain, the Bacillus subtilis (SC strain) strain and the Bacillus velezensis (Bacillus velezensis) WB strain into a beef extract peptone solid culture medium for activation, re-inoculating the activated strain WB into a beef extract peptone liquid culture medium, and then adjusting the strain concentration to OD (OD) by using sterile water 600 =1.0 asSeed liquid;
(2) Mixing activated bacterium liquids of LZP03, LZP02, WB and SC according to a volume ratio of 1.
Preferably, the step (2) further comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
preferably, in the step (2), seed liquids of the Bacillus pumilus (Bacillus pumilus) LZP02 strain, the Bacillus megaterium (Bacillus megaterium) LZP03 strain, the Bacillus subtilis (Bacillus subtilis) SC strain and the Bacillus belgii (Bacillus velezensis) WB strain are mixed according to a volume ratio of 1:1:1:1, after mixing, inoculating the mixture into sterilized wastewater to be treated for pig raising according to the inoculation amount of 1-2 vol%; the fermentation refers to fermentation culture at 30 ℃ and 120r/min for 24-96h.
Preferably, the pig raising wastewater after treatment has reduced content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus and pH value compared with the pig raising wastewater before treatment.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, a composite microbial agent obtained by constructing four strains of Bacillus pumilus (LZP 02), bacillus megaterium (LZP 03), bacillus Subtilis (SC) and Bacillus velezensis (WB) is used as a fermentation strain, and related experiments prove that the LZP03 (Bacillus megaterium) and the LZP02 (Bacillus velezensis) are used as a rice rhizosphere growth-promoting bacterium which can effectively promote the growth and development of rice seedlings and improve the nutrient absorption capacity of the rice seedlings, the WB (Bacillus velezensis) can remarkably inhibit the growth of crop fusarium oxysporum, improve the disease resistance of crops and promote the yield and income of crops, and the SC (Bacillus subtilis) has good capacity of preventing and controlling watermelon blight and is a high-efficiency biocontrol bacterium, so that the beneficial bacterium group is used for being inoculated in pig-raising wastewater to ferment and reduce pollutants in the pig-raising wastewater. Compared with the traditional method, the method has the advantages that the high COD resistance, the pH value reduction, the ammonia nitrogen, phosphorus, total nitrogen, total organic matters and other effects can be better achieved, beneficial microorganisms can be obtained through filtration after fermentation is finished to produce microbial bacterial manure, the cost of pollutants can be reduced, and economic benefits can be obtained at the same time.
In addition, the single-bacterium fermented microbial fertilizer is used, so that the situations of single effect, poor adaptability and the like of some products can be caused, the single-bacterium fermented microbial fertilizer cannot play the due role in the growth and development process of plants, and the functionality of the single-bacterium fermented microbial fertilizer is weakened, while the LZP03, LZP02, WB and SC strains find that the strains have no antagonistic action and different functions through a flat plate confrontation test, so that the core flora of the composite microbial fertilizer formed by combining the strains can effectively avoid the defects of the single-bacterium fermented microbial fertilizer, the adaptability of the fertilizer in soil is improved, the maximum release of the fertilizer efficiency is guaranteed, sufficient nutrition is provided for crops in multiple directions, the suitable growth environment is guaranteed, the produced composite microbial fertilizer has stronger and more diverse functions in the application broad spectrum, and can be better applied to agricultural production increase. Compared with single strain for pig raising wastewater fermentation, the growth speed of the LZP03-LZP02-WB-SC compound bacteria in the pig urine wastewater is higher, the maximum biomass can be reached within 48 hours, the fermentation period is greatly shortened, the yield is improved, and meanwhile, certain removal effect is achieved on pollutants such as COD (chemical oxygen demand), ammoniacal nitrogen, organic matters and the like existing in the pig raising wastewater.
Drawings
FIG. 1 shows the biomass of the single bacterial agent and the compound bacterial agent in the optimized pig-raising wastewater along with the change of time;
wherein, A: LZP02; b: LZP03; c: SC; d: WB; e: LZP03-LZP02-WB-SC compound bacteria;
FIG. 2 is a comparison of the variation trend and the reduction rate of total nitrogen, total carbon and total organic carbon in the supernatant of the complex microbial inoculum fermentation liquor;
FIG. 3 shows the pH variation trend of the complex microbial inoculum in an optimized culture medium along with the fermentation time;
FIG. 4 is a trend of the removal of COD in the pig wastewater in the complex microbial inoculum fermentation broth with time;
FIG. 5 shows the removal rate of ammoniacal nitrogen and phosphorus in pig wastewater by the composite microbial inoculum.
Strain preservation information:
the Bacillus pumilus LZP02 is named as Bacillus pumilus LZP02, is classified and named as Bacillus pumilus LZP02 respectively, is preserved in China center for type culture collection, is addressed to the university of Wuhan, china, has a strain preservation number of CCTCC NO.M 2018598, and has a preservation time of 2018, 9 months and 6 days.
The Bacillus megatherium LZP03 is named as Bacillus megaterium LZP03, is classified and named as Bacillus megaterium LZP03 respectively, is preserved in China center for type culture Collection, has the address of China, wuhan university, has the strain preservation number of CCTCC NO. M2018599, and has the preservation time of 2018, 9 months and 6 days.
The Bacillus subtilis strain SC is named as Bacillus subtilis SC, classified and named as Bacillus subtilis SC, is preserved in China center for type culture Collection, and is addressed to Wuhan university, wuhan, china, and the strain preservation number is CCTCC NO: m20211546, preservation time 12/6/2021.
The Bacillus beijerinckii strain WB is named as Bacillus velezensis WB and classified as Bacillus velezensis WB, is preserved in China center for type culture Collection, and is addressed to Wuhan university, wuhan, china, and the strain preservation number is CCTCC NO: m20211547, preservation time 12/6/2021.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.
Example 1 comparison of growth of Single and Complex microbial Agents in pig urine wastewater
1 materials and methods
1.1 sources of test strains
The strains used in the test are different kinds of pure culture beneficial microorganisms obtained by separation and screening of subject components, research shows that all strains are high-efficiency strains with better industrial application potential in the aspects of plant growth promotion, biological control, pollutant control and the like, all strains can rapidly grow in the pig raising wastewater and have better removal effect on ammonia nitrogen, phosphorus, organic matters and the like, the preservation place is a refrigerator at minus 80 ℃, all strains are classified and identified by strain morphology and 16SrDNA, and the identification list is shown in Table 1.
TABLE 1 test strains
1.2 physical and chemical characteristics of wastewater from pig raising
The swine waste water used in the test was obtained from a pig farm in the Fular base region of the Qiqiahall city, and the basic characteristics of the collected swine waste water are shown in Table 2.
TABLE 2 physical and chemical properties of original swine wastewater
1.3 Medium
Beef extract peptone medium: 10.00g of peptone, 3.00g of beef extract, 5.00g of NaCl, 20.00g of agar, and adding no agar into a liquid culture medium, wherein the volume of distilled water is up to 1000.00mL.
The pig raising wastewater culture medium after carbon-nitrogen ratio optimization: 10g of brown sugar and the volume of the pig raising wastewater is up to 1000.00mL.
1.4 methods of investigation
(1) Single strain fermentation culture
Beneficial microorganism strains LZP03, LZP02, WB and SC preserved at-80 ℃ are respectively inoculated into a beef extract peptone solid medium and cultured for 24h at 30 ℃ for activation. Inoculating the strains with 2-3 activated loops into a 250mL triangular flask filled with 100mL beef extract peptone liquid culture medium under aseptic operation conditions by using an inoculating loop, carrying out shaking culture at 30 ℃ and 120r/min, then taking each strain cultured to the same concentration (OD 600= 1.0) as seed solution, then inoculating activated bacterial solutions of LZP03, LZP02, WB and SC into 300mL pig-raising wastewater culture medium according to 1vol% of inoculation amount respectively, then carrying out shake-flask culture at 30 ℃ and 120r/min, sampling samples every 24h, and determining the growth condition of thalli of a single bacterium agent in the optimized pig-raising wastewater culture medium along with time;
(2) Fermentation culture of composite strain
Beneficial microorganism strains LZP03, LZP02, WB and SC preserved at-80 ℃ are respectively inoculated into a beef extract peptone solid medium and cultured for 24h at 30 ℃ for activation. The strains after 2-3 rings activation are inoculated in a 250mL triangular flask containing 100mL beef extract peptone liquid medium by using an inoculating ring under aseptic operation conditions, shaking culture is carried out at 30 ℃ and 120r/min, then each strain cultured to the same concentration (OD 600= 1.0) is used as seed liquid, then activated bacterial liquids of LZP03, LZP02, WB and SC are mixed according to a volume ratio of 1.
2. Results
In order to determine the change of biomass of the single microbial inoculum and the compound microbial inoculum in the culture medium along with time, the activated strains are inoculated in the optimized culture medium to carry out viable count determination by a gradient dilution plating method.
As shown in FIG. 1A, LZP02 was cultured in the optimized pig-raising wastewater culture medium for 72 hours to reach a maximum viable count of 4.07X 10 10 cfu/mL. As shown in FIG. 1B, the maximum viable count reached by LZP03 in the optimized pig wastewater culture medium for 96h was 4.26X 10 10 cfu/mL. As can be seen from FIG. 1C, SC strain was cultured in pig-raising wastewater culture medium with optimized carbon-nitrogen ratio for 48h to reach a maximum viable count of 4.16X 10 10 Viable count of cfu/mL. As can be seen from FIG. 1D, the WB strain reached a maximum biomass of 4.64X 10 after 48h in the original medium 10 cfu/mL. From FIG. 1E, it can be seen that the biomass of the complex microbial inoculum in the pig urine culture medium increases and then decreases and tends to be stable with time, and the maximum biomass of the viable bacteria after 48h of culture is 3.84 × 10 10 cfu/mL, and the subsequent biomass reduction may be due to the fact that brown sugar is used as a quick-acting carbon source by the microorganism but the biomass of the microorganism is reduced as the growth utilization is increased to change the carbon-nitrogen ratio so as to influence the growth of the microorganism.
Therefore, compared with the single strain for pig raising wastewater fermentation, the LZP03-LZP02-WB-SC compound strain has the advantages that the growth speed of the strain in the pig urine wastewater is higher, the maximum biomass can be reached within 48 hours, the fermentation period is greatly shortened, and the yield is improved.
Example 2 Compound microbial agent and application thereof in treatment of swine urine wastewater
1 materials and methods
1.1 sources of test strains
The same as in example 1.
1.2 physicochemical Properties of wastewater from pig raising
The swine waste water used in the test is taken from a certain pig farm in the Fulal base area in Qiqi Harer city, 1wt% of brown sugar is added into the collected swine waste water to optimize the carbon-nitrogen ratio to be used as a basic fermentation culture medium, and the basic characteristics of the optimized swine waste water are shown in Table 3.
TABLE 3 optimization of post-pig-raising wastewater physicochemical properties
1.3 Medium
Beef extract peptone medium: 10.00g of peptone, 3.00g of beef extract, 5.00g of NaCl, 20.00g of agar, and adding no agar into a liquid culture medium, wherein the volume of distilled water is up to 1000.00mL.
1.4 methods of investigation
Beneficial microorganism strains LZP03, LZP02, WB and SC preserved at-80 ℃ are respectively inoculated into a beef extract peptone solid medium and cultured for 24h at 30 ℃ for activation. The 2-3 loops activated strain was inoculated in a 250mL Erlenmeyer flask containing 100mL beef extract peptone liquid medium under aseptic conditions using an inoculating loop, and subjected to shaking culture at 30 ℃ at 120r/min, then each strain cultured to the same concentration (OD 600= 1.0) was used as a seed solution, and then activated bacterial solutions of LZP03, LZP02, WB, SC were mixed at a volume ratio of 1.
1.5 test measurement index
1.5.1 Water quality index
And (4) measuring the contents of pH, total nitrogen, total carbon, total organic carbon, ammoniacal nitrogen and phosphorus of the sample.
1.5.2 microbiological indicators
Placing 10mL of fermentation liquor into 90mL of sterile water, shaking for 30min in a shaking table at 200r/min, and then performing gradient dilution to obtain 10 -7 、10 -8 、10 -9 And (3) taking 0.1mL of the diluent, coating the diluent on a beef extract peptone medium plate, culturing at 30 ℃ for 24h, calculating the number of viable bacteria (cfu/mL), and setting three groups in parallel.
2. Results and discussion
2.1 Change of Each index of fermentation waste liquid after removing thallus from pig-raising waste water treated by composite microbial agent
2.1.1 Change of Total Nitrogen, total carbon and Total organic carbon content of the Compound microbial inoculum in the optimized pig-raising wastewater
And inoculating the compound microbial agent to an optimized culture medium, and measuring the total nitrogen of the fermentation liquor every 24 hours. As can be seen from FIG. 2, after the compound microbial inoculum is inoculated in the optimized culture medium, the total nitrogen content of the fermentation broth shows a general descending trend along with the prolonging of the fermentation time, the total nitrogen content is rapidly descended in a fermentation interval of 0-24 hours and then tends to be flat, the total nitrogen content reaches a minimum value of 102.2mg/L when the fermentation broth is cultured for 96 hours, and the total nitrogen content is descended by 73.55 percent compared with the control group. The change of the total carbon of the complex microbial inoculant is also obviously reduced as shown in FIG. 2. The total carbon content in the fermentation liquor shows a slow descending trend along with the increase of the culture time, the minimum value of the total carbon content in the fermentation liquor of the compound microbial inoculum is 3010mg/L, compared with a control group, the total carbon reduction rate of the fermentation liquor of the compound microbial inoculum after 96 hours of culture is 48.55%, the reduction of the total carbon content is probably caused by the fact that the total carbon content is reduced due to the fact that the microorganisms continuously perform aerobic respiration to discharge carbon dioxide in the growth process, and therefore the situation that the microorganisms can actively grow and reproduce in the pig-raising wastewater is shown. In the aspect of total organic carbon, the compound microbial inoculum is inoculated in the optimized pig-raising wastewater culture medium, the total organic carbon content of fermentation liquor continuously decreases along with the prolonging of the culture time and finally tends to be stable, the total organic carbon content of centrifugal fermentation liquor rapidly decreases in a fermentation period of 0-24h, reaches a minimum value in 48h and then tends to be stable after slightly increasing in 72h, and the minimum decrease rate of the total organic carbon of the compound microbial inoculum compared with a control group reaches 54.10%.
2.1.2 pH change of the optimized pig-raising wastewater by the composite microbial inoculum
The pH value of the fermentation liquor of the compound microbial agent is measured every 24 hours, and as can be seen from figure 3, after the pig-raising wastewater is fermented for 96 hours by the compound microbial agent, the pH value of the pig-raising wastewater is reduced from an original weak alkaline solution with pH =7.82 to a weak acidic fermentation liquor with pH =6.51, which is probably due to the fact that the thalli secrete organic acids, the pH value is reduced, and the method has important significance for threat prevention of the pig-raising wastewater to soil salinization.
2.1.3 change of COD content and removal rate of the compound microbial inoculum in the optimized pig-raising wastewater
In order to determine the effect of the complex microbial inoculum on removing COD from the pig raising wastewater, COD detection is carried out on the supernatant of the fermentation liquid, and as can be seen from figure 4, along with the continuous increase of the fermentation time of the strain, the COD content of the strain is continuously and rapidly reduced from original COD =27400 mg/L to COD =9360mg/L at the maximum, and the removal rate of the COD reaches 65.38%, which indicates that the complex microbial inoculum has a certain removal rate on the COD of the pig raising wastewater, and the threat of the filtered waste liquid on the environment is reduced.
2.1.4 removal rate of ammonia nitrogen and phosphorus in optimized pig raising wastewater by using compound microbial inoculant
The ammonia nitrogen content of the fermentation liquid supernatant of the compound microbial inoculum at different fermentation times is measured, and as can be seen from fig. 5, the compound microbial inoculum can greatly reduce the ammonia nitrogen content of the fermentation waste liquid, the removal rate of the ammonia nitrogen reaches 58.17%, and the removal effect of the ammonia nitrogen is good. After the fermentation, the phosphorus content of the supernatant of the fermentation liquor is measured, so that whether the strain can well reduce the phosphorus content removal rate in the fermentation waste liquor can reach 44.77 percent or not can be seen, and the strain has good phosphorus removal capability.
Claims (10)
1. The compound microbial agent is characterized by consisting of a Bacillus pumilus (Bacillus pumilus) LZP02 strain, a Bacillus megaterium (Bacillus megaterium) LZP03 strain, a Bacillus subtilis (Bacillus subtilis) SC strain and a Bacillus velezensis (Bacillus velezensis) WB strain, wherein the Bacillus pumilus LZP02 strain is preserved in a China center for type culture collection with the strain preservation number of CCTCC NO.M 2018598, the Bacillus megaterium LZP03 strain is preserved in a China center for type culture collection with the strain preservation number of CCTCC NO.M 2018599, the Bacillus subtilis (Bacillus subtilis) SC strain is preserved in a China center for type culture collection with the strain preservation number of CCTCC NO: m20211546, said Bacillus velezensis WB strain, preserve in China center for type culture Collection, its strain preservation number is CCTCC NO: m20211547.
2. The use of the complex microbial inoculant defined in claim 1 in the treatment of swine wastewater.
3. The application of claim 2, wherein seed solutions of the Bacillus pumilus (LZP 02), the Bacillus megaterium (LZP 03), the Bacillus Subtilis (SC) and the Bacillus belius (WB) are respectively inoculated into sterilized pig wastewater to be treated, and the treated pig wastewater is fermented to obtain the treated pig wastewater, wherein the pollutant content of the treated pig wastewater is reduced compared with that of the treated pig wastewater.
4. The use of claim 3, further comprising the step of adding brown sugar to the sterilized swine wastewater to be treated so that the swine wastewater has a carbon-to-nitrogen ratio of 16-20:1.
5. the use according to claim 3, wherein the seed liquid of Bacillus pumilus (Bacillus pumilus) LZP02 strain, bacillus megaterium (Bacillus megaterium) LZP03 strain, bacillus subtilis SC strain and Bacillus bleekensis (Bacillus velezensis) WB strain is mixed in a volume ratio of 1:1:1:1, mixing and inoculating the mixture into sterilized wastewater to be treated for pig raising; the fermentation refers to fermentation culture at 30 ℃ and 120r/min for 24-96h.
6. The use according to claim 3, wherein the swine wastewater after treatment has a reduced content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus compared to the swine wastewater before treatment.
7. A method for treating pig raising wastewater is characterized by comprising the following steps:
(1) Respectively inoculating the Bacillus pumilus (Bacillus pumilus) LZP02 strain, bacillus megaterium (Bacillus megaterium) LZP03 strain, bacillus subtilis (Bacillus subtilis) SC strain and Bacillus velezensis (Bacillus velezensis) WB strain as defined in claim 1 into a beef extract peptone solid medium for activation, inoculating the activated strain WB into a beef extract peptone liquid medium, and adjusting the strain concentration to OD using sterile water 600 =1.0 as seed liquid;
(2) Mix LZP03, LZP02, WB, SC's activation fungus liquid according to volume ratio 1.
8. The method as claimed in claim 7, wherein the step (2) further comprises the step of adding brown sugar to the sterilized swine wastewater to be treated so that the carbon-nitrogen ratio of the swine wastewater is 16-20:1.
9. the method according to claim 7, wherein in the step (2), seed liquids of Bacillus pumilus (Bacillus pumilus) LZP02 strain, bacillus megaterium (Bacillus megaterium) LZP03 strain, bacillus subtilis (Bacillus subtilis) SC strain and Bacillus bleezensis (Bacillus velezensis) WB strain are mixed in a volume ratio of 1:1:1:1, after mixing, inoculating the mixture into sterilized pig raising wastewater to be treated according to the inoculation amount of 1-2 vol%; the fermentation refers to fermentation culture at 30 ℃ and 120r/min for 24-96h.
10. The method of claim 7, wherein the swine wastewater after treatment has a reduced content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus and pH compared to the swine wastewater before treatment.
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