CN115104441B - Efficient rooting method for blueberry twig cuttage - Google Patents
Efficient rooting method for blueberry twig cuttage Download PDFInfo
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- CN115104441B CN115104441B CN202210967200.9A CN202210967200A CN115104441B CN 115104441 B CN115104441 B CN 115104441B CN 202210967200 A CN202210967200 A CN 202210967200A CN 115104441 B CN115104441 B CN 115104441B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Cultivation Of Plants (AREA)
Abstract
The invention adopts pine needle soil and coarse sand as cutting substrates, IAA and IBA are used in combination with vitamin B12 to promote rooting, and the pine needle soil and coarse sand are low in price, so that planting cost can be saved for enterprises.
Description
Technical Field
The invention relates to the technical field of blueberry cutting, in particular to an efficient rooting method for blueberry twig cutting.
Background
As known, blueberry (academic name: vaccinum spp.) is commonly known as a plant of the rhododendron genus and the blueberry genus, perennial low shrubs, shrubs are clustered, the difference of the size and morphology of the tree is remarkable, blueberry fruits are generally ripe 70-90 days after flowers, pulp is thin and soft, juice is rich, seeds are fine, taste is not affected when eating along with pulp, fruits are dark blue or violet when most varieties are ripe, such as rabbit eye blueberries, high cluster blueberries, short cluster blueberries and the like, and few varieties are red.
The blueberry is used as an emerging small berry tree species with higher economic value and wide development prospect, has fine fruit quality, extremely small seeds, palatable sweetness and sourness and fresh and pleasant fragrance, belongs to fruits with high amino acid content, high zinc content, high calcium content, high molten iron content and the like, is rich in nutrients such as multiple vitamins and trace elements, and the content of vitamin E in fresh fruits is several times or even tens times of that of apples, grapes and the like, and is known as the king of berries. The fresh blueberry fruit can be eaten raw, can be used as a raw material for processing fruit juice, fruit wine, jam and the like, and has higher economic value. Besides the conventional nutritional ingredients, the blueberries also contain rich flavonoids, polysaccharide compounds and anthocyanin pigment, so that the blueberry has good effects of protecting eyesight, protecting cardiovascular and cerebrovascular, reducing blood sugar and enhancing immunity, and has great market demands.
Along with the continuous increase of the market demand of blueberries, high-quality varieties are required to be rapidly propagated by blueberry planting enterprises in China, indoor tissue culture is adopted in the existing propagation technology, the cost is high, seedling hardening and transplanting are required, the time consumption is long, matrix is required to be irrigated by nutrient solution in the out-of-bottle cutting technology, the planting cost of the enterprises is increased, and the like.
Disclosure of Invention
In order to overcome the defects in the background art, the invention provides a high-efficiency rooting method for blueberry twig cuttage, which directly adopts pine needle soil and coarse sand as cuttage matrixes, uses IAA and IBA two hormones in combination with vitamin B12 to promote rooting, has short rooting time, and can greatly reduce the planting cost of enterprises.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
the efficient rooting method for blueberry twig cuttage specifically comprises the following steps:
firstly, preparing a cutting matrix:
mixing loose soil and coarse sand according to a ratio of 1:1 to obtain a cutting matrix, spraying potassium permanganate to the cutting matrix to disinfect the cutting matrix, sealing the disinfected cutting matrix with a film for 3-6 h, and then wetting with an aqueous solution with pH=4.5-5.5;
secondly, preparing strong branches of blueberries:
selecting strong blueberry branches in spring, sterilizing scissors by using 70% -80% alcohol, then shearing the branches which are lignified in the current year and have the diameter of 0.3-0.5 cm by using the scissors, flushing for 25-35 min under running water, and drying the moisture on the branches in the shade; then cutting off the non-lignified part of the morphological upper end of the branch, and trimming the rest part into scions with the length of 10-15 cm;
thirdly, pruning branches:
pruning the branches processed in the second step into a lower notch with a 45-degree wedge shape, flatly cutting the upper notch at a position 1-2 cm away from the uppermost leaf, removing the leaf at the lower end of the scion, only retaining 2-3 leaves at the upper end, cutting the retained leaves from the middle, retaining only half of the leaves connected with the branches, and reducing the transpiration and water loss of the leaves;
fourthly, soaking the base parts of the branches:
cutting the branches into wedge-shaped parts with one end of 5-8 cm, soaking the wedge-shaped parts in a solution containing 50-100 mg/L IAA, 50-100 mg/L IBA and 0.5-2% vitamin B12 for 8-12 h, and airing for standby;
fifthly, cutting and rooting:
soaking the wedge-shaped notch of the branch treated in the previous step in 1-3% potassium permanganate solution for 10-30 s, then cutting the wedge-shaped notch into the cutting matrix prepared in the first step, wherein the insertion depth is 5-10 cm, then performing dark culture for 3-7 days in an environment with the humidity of 60-90%, and finally performing rooting culture for 20-30 days under the conditions of 22-28 ℃ and 1500-2000 xl illumination and 16h/d, wherein rooting is completed.
The method for obtaining the pine needle soil in the first step is that the dried pine needle and a proper amount of soil under the pine tree are put into a large plastic bag, the plastic bag is wetted and kept moist, and fermentation is carried out after the plastic bag is sealed.
The blueberry twig cuttage efficient rooting method is characterized in that the dried pine needles are collected in autumn.
The method for efficiently rooting the blueberry twig cuttage comprises the steps of removing the non-rotted pine needles under the pine tree, and digging and removing pale yellow or light yellow brown leaf rot soil at the covered part of the pine needles.
The particle size of the coarse sand in the first step is 0.4-0.6 mm.
According to the efficient rooting method for blueberry twig cuttage, the concentration of potassium permanganate in the first step is 0.05% -0.15%.
According to the efficient rooting method for blueberry twig cuttage, in the first step, white vinegar is added into clear water, and the pH value of the clear water is adjusted to be 4.5-5.5 by using the white vinegar.
According to the efficient rooting method for blueberry twig cuttage, the length of the non-lignified part at the morphological upper end of the branch in the second step is 5-8 cm.
By adopting the technical scheme, the invention has the following advantages:
according to the invention, the pine needle soil and coarse sand are adopted as cutting matrixes, IAA and IBA are used for promoting rooting, and the IAA and IBA are matched with vitamin B12, so that the pine needle soil and coarse sand are low in price, the planting cost can be saved for enterprises, meanwhile, the spring blueberry branch cutting is adopted, the spring blueberry branch cutting is accompanied, the branch is directly cut by the trimmed branch, the branch is not wasted, and the pH value of the cutting matrixes is 4.5-5.5, so that the blueberry is suitable for growing, the rooting rate of scions is effectively improved, and the potassium permanganate is used for soaking, so that the scions are not easy to rot in matrix parts, and then matched with conditions such as dark culture, the efficient rooting is realized, the method is suitable for blueberry cutting seedling raising, a good method is provided for blueberry planting enterprises, a simple and effective rooting technology is provided for blueberry planting enterprises, the cost of enterprises is reduced, and the method is suitable for popularization and application in a large range.
Detailed Description
The present invention will be explained in more detail by the following examples, which are not intended to limit the scope of the invention;
the invention relates to a high-efficiency rooting method for blueberry twig cuttage, which specifically comprises the following steps:
firstly, preparing a cutting matrix:
mixing loose soil and coarse sand according to a ratio of 1:1 to obtain a cutting matrix, spraying potassium permanganate to the cutting matrix to disinfect the cutting matrix, sealing the disinfected cutting matrix with a film for 3-6 h, and then wetting with an aqueous solution with pH=4.5-5.5; then watering the scions is carried out by adopting water with the pH value;
in specific implementation, the pine needle soil obtaining method comprises the steps of putting the pine needle dried under the pine tree and a proper amount of soil into a large plastic bag, wetting and keeping moist, sealing the plastic bag, and fermenting; when fermenting, the plastic bag can be put in a place with higher temperature for fermenting after being sealed; wherein the dry pine needles are dry pine branches collected in autumn;
further, the alternative method for obtaining the pine needle soil is to remove the non-rotted pine needle under the pine tree, dig and remove the pale yellow or yellowish brown leaf rot soil at the covered part of the pine needle;
further, the particle size of the coarse sand is 0.4-0.6 mm, and when the method is implemented, the particle size of the coarse sand is preferably 0.5mm;
further, the concentration of the potassium permanganate is 0.05% -0.15%;
further, the aqueous solution is prepared by adding white vinegar into clear water, and adjusting the pH value of the clear water to be 4.5-5.5 by using the white vinegar;
secondly, preparing strong branches of blueberries:
selecting strong blueberry branches in spring, sterilizing scissors by using 70% -80% alcohol, then shearing the branches which are lignified in the current year and have the diameter of 0.3-0.5 cm by using the scissors, flushing for 25-35 min under running water, and drying the moisture on the branches in the shade; then cutting off the non-lignified part of the morphological upper end of the branch, and trimming the rest part into scions with the length of 10-15 cm; the length of the non-lignified part at the morphological upper end of the branch is 5-8 cm;
thirdly, pruning branches:
pruning the branches processed in the second step into a lower notch with a 45-degree wedge shape, flatly cutting the upper notch at a position 1-2 cm away from the uppermost leaf, removing the leaf at the lower end of the scion, only retaining 2-3 leaves at the upper end, cutting the retained leaves from the middle, retaining only half of the leaves connected with the branches, and reducing the transpiration and water loss of the leaves; generally, long branches can cut three scions up, middle and lower;
fourthly, soaking the base parts of the branches:
cutting the branches into wedge-shaped parts with one end of 5-8 cm, soaking the wedge-shaped parts in a solution containing 50-100 mg/L IAA, 50-100 mg/L IBA and 0.5-2% vitamin B12 for 8-12 h, and airing for standby; in the implementation, the components of the soaking solution are simple, no complex nutritional components or mineral substances exist, the rooting rate is greatly reduced due to the fact that the IAA and IBA in the soaking solution are too high or too low in concentration, the two hormones are matched with vitamin B12 with a certain concentration for use under a proper concentration, the highest rooting rate can reach 87% in experiments, and the rooting rate can reach more than 80% in two different blueberry varieties of rabbit eyes and duke;
fifthly, cutting and rooting:
soaking wedge-shaped cut parts of the branches treated in the previous step in 1-3% potassium permanganate solution for 10-30 s, and then cutting the wedge-shaped cut parts into the cutting matrix prepared in the first step, wherein the insertion depth is 5-10 cm and accounts for 2/3 of the whole scion; the scion cutting can be densely planted, the plant spacing is about 3cm multiplied by 3cm, (the densely planted method has the advantages of saving the matrix or space and reducing the enterprise cost), then the scion cutting is firstly dark-cultured for 3 to 7 days in an environment with the humidity of 60 to 90 percent, and finally rooting culture is carried out for 20 to 30 days under the conditions of 22 to 28 ℃ and 1500 to 2000xl illumination and 16 hours/d, and the rooting is completed at the moment.
In the concrete implementation of the invention, the scion is firstly dark-cultured for 7 days in the environment with the humidity of 60-90%, the rooting culture is carried out under the conditions of maintaining the laboratory temperature at 22-28 ℃ and illumination at 1500-2000 xl and 16h/d, watering is carried out once every 5 days, the substrate is always kept moist, and the watering water adopts clear water and white vinegar, and the pH value is 4.5-5.5.
Effect of different concentrations on maytansinol cutting rooting
The two tables adopt two different blueberry varieties, and when the screened IAA and IBA are 100mg/L, the two blueberry varieties are matched with vitamin B12 for use, the rooting rate is over 80 percent, and the blueberry cutting rooting method is used in different blueberry varieties.
The invention is not described in detail in the prior art.
The embodiments selected herein for the purposes of disclosing the present invention are presently considered to be suitable, however, it is to be understood that the present invention is intended to include all such variations and modifications as fall within the spirit and scope of the present invention.
Claims (8)
1. A blueberry twig cuttage efficient rooting method is characterized by comprising the following steps: the rooting method specifically comprises the following steps:
firstly, preparing a cutting matrix:
mixing loose soil and coarse sand according to a ratio of 1:1 to obtain a cutting matrix, spraying potassium permanganate to the cutting matrix to disinfect the cutting matrix, sealing the disinfected cutting matrix with a film for 3-6 h, and then wetting with an aqueous solution with pH=4.5-5.5;
secondly, preparing strong branches of blueberries:
selecting strong blueberry branches in spring, sterilizing scissors by using 70% -80% alcohol, then shearing the branches which are lignified in the current year and have the diameter of 0.3-0.5 cm by using the scissors, flushing for 25-35 min under running water, and drying the moisture on the branches in the shade; then cutting off the non-lignified part of the morphological upper end of the branch, and trimming the rest part into scions with the length of 10-15 cm;
thirdly, pruning branches:
pruning the branches processed in the second step into a lower notch with a 45-degree wedge shape, flatly cutting the upper notch at a position 1-2 cm away from the uppermost leaf, removing the leaf at the lower end of the scion, only retaining 2-3 leaves at the upper end, cutting the retained leaves from the middle, retaining only half of the leaves connected with the branches, and reducing the transpiration and water loss of the leaves;
fourthly, soaking the base parts of the branches:
cutting the branches into wedge-shaped parts with one end of 5-8 cm, soaking the wedge-shaped parts in a solution containing 50-100 mg/L IAA, 50-100 mg/L IBA and 0.5-2% vitamin B12 for 8-12 h, and airing for standby;
fifthly, cutting and rooting:
soaking the wedge-shaped notch of the branch treated in the previous step in 1-3% potassium permanganate solution for 10-30 s, then cutting the wedge-shaped notch into the cutting matrix prepared in the first step, wherein the insertion depth is 5-10 cm, then performing dark culture for 3-7 days in an environment with the humidity of 60-90%, and finally performing rooting culture for 20-30 days under the conditions of 22-28 ℃ and 1500-2000 xl illumination and 16h/d, wherein rooting is completed.
2. The efficient rooting method for blueberry twig cuttage of claim 1, which is characterized by comprising the following steps: the method for obtaining the pine needle soil in the first step comprises the steps of taking the pine needle dried under the pine tree and filling a proper amount of soil into a large plastic bag, wetting and keeping moist, sealing the plastic bag, and fermenting.
3. The efficient rooting method for blueberry twig cuttage of claim 2, which is characterized by comprising the following steps of: the dry pine needles are dry pine branches collected in autumn.
4. The efficient rooting method for blueberry twig cuttage of claim 1, which is characterized by comprising the following steps: the alternative method for obtaining the loose needle soil in the first step is to remove the non-rotted loose needles under the pine tree, dig and remove pale yellow or light yellow brown leaf rot soil at the covered parts of the loose needles.
5. The efficient rooting method for blueberry twig cuttage of claim 1, which is characterized by comprising the following steps: the grain diameter of the coarse sand in the first step is 0.4-0.6 mm.
6. The efficient rooting method for blueberry twig cuttage of claim 1, which is characterized by comprising the following steps: the concentration of potassium permanganate in the first step is 0.05% -0.15%.
7. The efficient rooting method for blueberry twig cuttage of claim 1, which is characterized by comprising the following steps: the first step of adding white vinegar into clear water, and adjusting the pH value of the clear water to be 4.5-5.5 by using the white vinegar.
8. The efficient rooting method for blueberry twig cuttage of claim 1, which is characterized by comprising the following steps: the length of the non-lignified part at the morphological upper end of the branch in the second step is 5-8 cm.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002262665A (en) * | 2001-03-08 | 2002-09-17 | Satoru Nagisa | Method for proliferating blueberry by graft cutting thereof |
| CN104756724A (en) * | 2015-04-15 | 2015-07-08 | 四川培培生态农业有限公司 | Blueberry green branch cutting and rooting method |
| CN105284388A (en) * | 2015-11-18 | 2016-02-03 | 四川农业大学 | Blueberry softwood cutting seedling method |
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- 2022-08-12 CN CN202210967200.9A patent/CN115104441B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002262665A (en) * | 2001-03-08 | 2002-09-17 | Satoru Nagisa | Method for proliferating blueberry by graft cutting thereof |
| CN104756724A (en) * | 2015-04-15 | 2015-07-08 | 四川培培生态农业有限公司 | Blueberry green branch cutting and rooting method |
| CN105284388A (en) * | 2015-11-18 | 2016-02-03 | 四川农业大学 | Blueberry softwood cutting seedling method |
Non-Patent Citations (1)
| Title |
|---|
| 蓝莓嫩枝在不同基质扦插繁育试验;史德胜;;农业工程技术(第11期);全文 * |
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