CN114027132A - Method for producing potato breeder's seed by using fungus stick compound matrix - Google Patents

Method for producing potato breeder's seed by using fungus stick compound matrix Download PDF

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Publication number
CN114027132A
CN114027132A CN202111305202.3A CN202111305202A CN114027132A CN 114027132 A CN114027132 A CN 114027132A CN 202111305202 A CN202111305202 A CN 202111305202A CN 114027132 A CN114027132 A CN 114027132A
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compound matrix
matrix
fungus
seedlings
temperature
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张红骥
于德才
赵晓松
刘平杰
欧坤玉
普金
张丽
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Yunnan Agricultural University
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Yunnan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The method for producing the potato breeder seeds by utilizing the bacterium rod compound matrix comprises the steps of firstly preparing the bacterium rod compound matrix, smashing fresh bacterium rods, piling and retting, carrying out sealed anaerobic fermentation for 2 days, changing bacterium powder in the bacterium rods from white to black, then uniformly mixing the fermented bacterium rods and raw soil with the same volume to serve as the compound matrix, pouring water into the compound matrix until the compound matrix is saturated, keeping the temperature for 1-2 d when the temperature of the compound matrix reaches 60 ℃, continuing to ferment the compound matrix, circulating the process for at least 2 times, completely fermenting the compound matrix, reducing the temperature of the bacterium rod compound matrix to 20-30 ℃, and finishing the preparation of the bacterium rod compound matrix within 5-7 d; then, transplanting tissue culture seedlings, and managing the transplanted seedlings to enable the survival rate of the seedlings to reach 99%; covering soil when the stolons of the potatoes grow to 6-10 cm, covering all the stolons, and managing according to a conventional potato original seed cultivation process. The method effectively utilizes the fungus stick waste, and improves the quality and the yield of the potato breeder's seed.

Description

Method for producing potato breeder's seed by using fungus stick compound matrix
Technical Field
The invention belongs to the technical field of agricultural cultivation, and particularly relates to a production method of potato breeder seeds.
Background
Matrix cultivation is the most extensive cultivation mode for potato breeder's seed production at present. The substrate cultivation is cultivation in which solid matters such as grass peat, gravel, agricultural and forestry wastes and the like replace soil, and nutrient solution controlled manually provides nutrients and water for crops, so that plants can grow normally and the whole cultivation period is completed. The plants cultivated by the matrix are not easy to fall down, the root system grows in the matrix, and the matrix has good air permeability and is beneficial to the growth of the root system. In the cultivation process, water and fertilizer are poured into the matrix and are kept by the matrix, so that the loss is less and the utilization rate is high. The substrate has a certain buffering effect on the change of the nutrient solution, and the slight change of the concentration, the pH value and the like of the nutrient solution generally cannot cause obvious adverse effects on plants. Therefore, the substrate cultivation is beneficial to the growth of the root system of the virus-free potato seedling and can effectively improve the potato bearing rate of the potato breeder's seed.
At present, the following matrixes are mainly adopted for cultivating the potato virus-free seedlings, namely the first matrix: the matrix prepared from vermiculite and perlite according to the proportion of 1:1, and the second: a substrate prepared by vermiculite and turf according to a ratio of 1:1, and a third substrate: vermiculite, ash and decomposed cow dung are mixed according to the proportion of 8: 1:1 ratio of the prepared matrix. The vermiculite is added into the matrix, belongs to the matrix with larger air gaps, is easy to mix uniformly, has better water and fertilizer retention, but has higher cost price, is fragile and cannot be used continuously, and has influence on air permeability and high pH value when being used continuously.
The waste mushroom sticks are used as waste raw materials after the edible mushrooms are planted, mainly comprise sawdust, cottonseed hulls, straws and the like, are still rich in N, P, K and other nutrient elements, and have a non-negligible nutritional value. At present, the treatment and the reutilization of the waste fungus sticks in China mainly focus on secondary utilization or the utilization of the waste fungus sticks as organic fertilizers, culture mediums, animal feeds, fuels, methane tank fermentation materials and the like. The waste mushroom sticks are rich in organic matters which can be absorbed by plants, and are rich in mycelium residues, so that the waste mushroom sticks are loose in texture and have stronger water absorption and fertilizer retention than perlite, and can be secondarily utilized as a culture medium.
Disclosure of Invention
The invention provides a method for producing potato breeder seeds by using a fungus stick compound matrix, which can effectively utilize fungus stick waste, improve the quality of potatoes and improve the yield of potatoes.
The technical scheme adopted by the invention is as follows:
the method for producing the potato breeder seeds by using the fungus stick compound matrix is carried out in a cultivation greenhouse, a shading net is covered on a greenhouse top, the fungus stick compound matrix is manufactured, tissue culture seedlings are transplanted, and then transplanting seedling management is carried out, wherein the method comprises the following steps:
(1) preparing a fungus stick compound matrix:
1) crushing the fungus sticks: crushing fresh mushroom sticks;
2) primary fermentation treatment: piling and retting the crushed fungus rod materials, sealing and performing anaerobic fermentation for 2 days, wherein the fungus powder in the fungus rod materials is changed from white to black;
3) secondary fermentation treatment: uniformly mixing the primary fermented mushroom stick material with raw soil with the same volume to serve as a compound matrix, paving the compound matrix on a seedbed, watering the compound matrix until the compound matrix is saturated, and keeping the temperature for 1-2 d when the temperature of the compound matrix reaches 60 ℃ so as to continuously ferment the compound matrix; watering the compound matrix to saturation, and continuously keeping the temperature for 1-2 d when the temperature of the compound matrix reaches 60 ℃ so that the compound matrix is continuously fermented; the fermentation is continued for at least 2 times in such a circulating way, and the compound substrate is completely fermented;
4) reducing the temperature in the shed to reduce the temperature of the fermented fungus stick compound matrix to 20-30 ℃;
completing the preparation of the fungus stick compound matrix within 5-7 days;
(2) transplanting the tissue culture seedlings, wherein the method comprises the following steps:
1) seedling pouring: selecting a thick and strong tissue culture seedling without pollution, pouring the tissue culture seedling out of a culture bottle to avoid the stem from being broken, and cleaning a culture medium remained on a root system after taking out;
2) transplanting: fully watering the bacterium rod compound matrix until the bacterium rod compound matrix is saturated before transplanting the tissue culture seedlings, and directly transplanting the tissue culture seedlings into the bacterium rod compound matrix;
(3) managing transplanted seedlings: checking the growth vigor of the seedlings every 1d, watering for 2 times a day, timely preventing and treating diseases, pests, weeds and rats, culturing for 10 d-15 d, removing the shading net, reducing the watering times and the watering amount, and ensuring that the survival rate of the seedlings reaches 99%; covering soil when the stolons of the potatoes grow to 6-10 cm, covering all the stolons, and managing according to a conventional potato original seed cultivation process.
The invention adopts the bacterium rod compound matrix to cultivate the potato original seeds, the bacterium rod compound matrix has high nutrient content, can effectively inhibit bacteria, has low cost, better improves the physicochemical property of soil, balances the vegetative growth and reproductive growth and improves the potato yield, and is particularly shown in the following aspects:
(1) the nutrient content of the fungus stick compound matrix is high, which is beneficial to improving the yield of the potatoes. Wherein the organic matter content of the new substrate is 107 percent, compared with the seedling substrate, the organic matter content is increased by 52.3 percent, and the seedling substrate is commonly used in the market (the organic matter content is more than 46 percent). The total nitrogen content is 0.841%, which is increased by 55.7% compared with the seedling substrate. The total phosphorus content is 0.299, and is increased by 15.7 percent compared with the seedling culture substrate. The total potassium content is 2.53, which is increased by 78.3% compared with the seedling substrate. During the whole growth period of the potatoes, less fertilizer is needed in the seedling stage, the fertilizer requirement is increased in the bud stage, and the fertilizer requirement is reduced in the potato bearing stage, so that the fertilizer is in a unimodal curve. The three factors of nitrogen, phosphorus and potassium are absorbed in each growth stage of the potatoes, the absorption ratio of the nitrogen, phosphorus and potassium is 1:0.5:2.5, the potatoes absorb potassium most, nitrogen is secondly absorbed, and phosphorus is minimum. The bacterial stick complex matrix can effectively increase the total potassium content, ensure the continuous generation of starch and sugar and increase the potato production rate of the potato original seed.
(2) The bacterium rod compound matrix can effectively inhibit bacteria. The temperature is rapidly increased in the fermentation process of the fungus stick compound matrix, so that harmful germs in raw soil can be inhibited, worm eggs are killed by high-temperature decomposition, plant diseases and insect pests are reduced, and medicine residues are degraded. The content of beneficial bacteria after fermentation is increased, so that the soil structure can be improved, and soil hardening can be prevented.
(3) The fungus stick compound matrix can effectively reduce the cost. The common substrate perlite for cultivating the potato tissue culture seedlings is high in vermiculite price, and the cultivation substrate made of the selected mushroom sticks can save 3-5 yuan per square meter. Meanwhile, the culture medium prepared from the fungus sticks has high nutrient content, and the use of fertilizers can be reduced in the process of cultivating the potato breeder seeds, so that the cost can be effectively reduced by selecting the fungus stick compound medium in the production of the potato breeder seeds.
(4) The zymophyte stick can better improve the physical and chemical properties of soil. The fermented fungus sticks can increase the content of organic matters in soil, increase the air permeability of the soil and improve the temperature of the soil. The soilless culture of the fungus sticks is obviously superior to the common soil culture in the aspects of root length, root number, root dry weight and the like, and the fungus sticks have the advantages of fast rooting, fast growth and much dry matter accumulation of root systems.
Detailed Description
The present invention will be further illustrated with reference to the following examples.
The method for producing the potato breeder's seed by using the fungus stick compound matrix comprises the following steps:
1. building a shed: constructing an arched shed in a place with flat terrain, close to a water source and without tall trees nearby, covering a shading net on the shed roof, digging a drainage channel in the middle of the ground in the shed, and paving grass-prevention cloth on the seedbed ground at two sides of the drainage channel to prevent the primary weeds in the shed from influencing the growth of crops; the sprinkling irrigation system is installed in the shed, the sprinkling irrigation water is required to be clean and pollution-free, and the polluted river water is forbidden by using tap water, well water or mountain spring water, so that the occurrence of diseases is prevented.
2. The method for preparing the fungus stick compound matrix comprises the following steps:
1) crushing the fungus sticks: crushing fresh mushroom sticks into small pieces by using a crusher;
2) primary fermentation treatment: and (3) piling and retting the crushed bacteria bar, sealing and performing anaerobic fermentation for 2 days, wherein the bacteria powder in the bacteria bar is changed from white to black.
3) Secondary fermentation treatment: uniformly mixing the primary fermented mushroom stick material with raw soil with the same volume to serve as a compound matrix, paving the compound matrix on a seedbed, watering the compound matrix until the compound matrix is saturated, and keeping the temperature for 1-2 d when the temperature of the compound matrix reaches 60 ℃ so as to continuously ferment the compound matrix; watering the compound matrix to saturation, and continuing to keep the temperature for 1-2 d when the temperature of the compound matrix reaches 60 ℃ so that the compound matrix is fermented again; the fermentation is continued for at least 2 times in such a circulating way, and the compound substrate is completely fermented;
4) ventilating to reduce the temperature in the shed, and reducing the temperature of the fermented fungus stick compound matrix to 20-30 ℃;
and finishing the preparation of the fungus stick compound matrix within 5-7 days.
The unfermented fungus sticks are white large blocks, the fermented fungus sticks are black small crushed particles, and the unfermented fungus sticks of the white blocks cannot be used for planting, because a large amount of heat is generated in the fermentation process of the fungus sticks, and the seedlings are easy to burn.
3. Transplanting the tissue culture seedlings, wherein the method comprises the following steps:
1) seedling pouring: selecting a thick and strong tissue culture seedling without pollution, pouring the tissue culture seedling out of a culture bottle to avoid the stem from being broken, and cleaning a culture medium remained on a root system after taking out;
2) transplanting: because the fungus stick compound matrix has very strong water absorption, the fungus stick compound matrix needs to be fully watered until the fungus stick compound matrix is saturated before the tissue culture seedling is transplanted, and then the tissue culture seedling is directly transplanted into the fungus stick compound matrix, so that the survival rate of the tissue culture seedling is ensured.
4. Managing transplanted seedlings: the growth vigor of the seedlings is checked every 1d, watering is carried out for 2 times every day, diseases, pests, weeds and rats are found to be timely prevented and controlled, the shading net is removed after cultivation is carried out for 10 d-15 d, the watering times and the watering amount are properly reduced, and the survival rate of the seedlings can reach 99%. Covering soil when the stolons of the potatoes grow to 6-10 cm, covering all the stolons, and managing according to a conventional potato original seed cultivation process.
The waste of the waste mushroom sticks is reduced, the mushroom stick materials are fully fermented by adopting the mushroom stick compound matrix to cultivate the tissue culture seedlings, the plant diseases and insect pests in the matrix are killed, the subsequent seedling burning is prevented, and meanwhile, the process conditions for preparing the mushroom stick compound matrix are strictly controlled, so that the potato bearing rate of the potato breeder seeds is effectively improved, and the yield and the quality of the potato breeder seeds are improved. Compared with the prior art, the potato bearing rate of the potato original seed is obviously improved, the average bearing rate of 3 green potatoes 9 and 4.5 sweet potatoes 6 is improved, and the bearing rate of 88 cooperative potatoes is more 5, so that the bearing rate is improved by about 20-80 percent compared with that of the potato cultured by the traditional matrix. The invention has good application prospect.

Claims (2)

1. The method for producing the potato breeder seeds by using the fungus stick compound matrix is characterized in that the method is carried out in a cultivation greenhouse, a shading net is covered on the top of the greenhouse firstly, the fungus stick compound matrix is manufactured, tissue culture seedlings are transplanted, then transplanting seedling management is carried out, and the method comprises the following steps:
(1) preparing a fungus stick compound matrix:
1) crushing the fungus sticks: crushing fresh mushroom sticks;
2) primary fermentation treatment: piling and retting the crushed fungus rod materials, sealing and performing anaerobic fermentation for 2 days, wherein the fungus powder in the fungus rod materials is changed from white to black;
3) secondary fermentation treatment: uniformly mixing the primary fermented mushroom stick material with raw soil with the same volume to serve as a compound matrix, paving the compound matrix on a seedbed, watering the compound matrix until the compound matrix is saturated, and keeping the temperature for 1-2 d when the temperature of the compound matrix reaches 60 ℃ so as to continuously ferment the compound matrix; watering the compound matrix to saturation, and continuously keeping the temperature for 1-2 d when the temperature of the compound matrix reaches 60 ℃ so that the compound matrix is continuously fermented; the fermentation is continued for at least 2 times in such a circulating way, and the compound substrate is completely fermented;
4) reducing the temperature in the shed to reduce the temperature of the fermented fungus stick compound matrix to 20-30 ℃;
completing the preparation of the fungus stick compound matrix within 5-7 days;
(2) transplanting the tissue culture seedlings, wherein the method comprises the following steps:
1) seedling pouring: selecting a thick and strong tissue culture seedling without pollution, pouring the tissue culture seedling out of a culture bottle to avoid the stem from being broken, and cleaning a culture medium remained on a root system after taking out;
2) transplanting: fully watering the bacterium rod compound matrix until the bacterium rod compound matrix is saturated before transplanting the tissue culture seedlings, and directly transplanting the tissue culture seedlings into the bacterium rod compound matrix;
(3) managing transplanted seedlings: checking the growth vigor of the seedlings every 1d, watering for 2 times a day, timely preventing and treating diseases, pests, weeds and rats, culturing for 10 d-15 d, removing the shading net, reducing the watering times and the watering amount, and ensuring that the survival rate of the seedlings reaches 99%; covering soil when the stolons of the potatoes grow to 6-10 cm, covering all the stolons, and managing according to a conventional potato original seed cultivation process.
2. The method for producing potato breeder seeds by utilizing the fungus stick compound matrix as claimed in claim 1, wherein a cultivation greenhouse is built, a place with a flat terrain, a position close to a water source and no tall trees nearby is selected, a drainage channel is dug in the middle of the ground in the greenhouse, seedbed grounds on two sides of the drainage channel are paved with grass-proof cloth, a sprinkler irrigation system is installed in the greenhouse, and water for sprinkler irrigation is kept clean and pollution-free.
CN202111305202.3A 2021-11-05 2021-11-05 Method for producing potato breeder's seed by using fungus stick compound matrix Pending CN114027132A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105384529A (en) * 2015-11-30 2016-03-09 王教义 Potato mini-potato organic culture medium and preparation method
CN105601356A (en) * 2016-02-29 2016-05-25 山东省农业科学院农业资源与环境研究所 Method for comprehensive utilization of edible fungus residues
CN107439199A (en) * 2017-08-31 2017-12-08 迪庆州农业科学研究所 A kind of virus-free potato primary stock efficient breeding method
CN113197077A (en) * 2021-06-21 2021-08-03 云南农业大学 Floating hardening method for potato test-tube plantlets

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105384529A (en) * 2015-11-30 2016-03-09 王教义 Potato mini-potato organic culture medium and preparation method
CN105601356A (en) * 2016-02-29 2016-05-25 山东省农业科学院农业资源与环境研究所 Method for comprehensive utilization of edible fungus residues
CN107439199A (en) * 2017-08-31 2017-12-08 迪庆州农业科学研究所 A kind of virus-free potato primary stock efficient breeding method
CN113197077A (en) * 2021-06-21 2021-08-03 云南农业大学 Floating hardening method for potato test-tube plantlets

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
董飞等: "保护地马铃薯无土(菌棒代土)栽培试验研究", 《农业科技通讯》 *

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