CN113951135A - Screening method of saline-alkali tolerant plants - Google Patents
Screening method of saline-alkali tolerant plants Download PDFInfo
- Publication number
- CN113951135A CN113951135A CN202111234042.8A CN202111234042A CN113951135A CN 113951135 A CN113951135 A CN 113951135A CN 202111234042 A CN202111234042 A CN 202111234042A CN 113951135 A CN113951135 A CN 113951135A
- Authority
- CN
- China
- Prior art keywords
- screened
- saline
- plant
- plants
- alkali
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003513 alkali Substances 0.000 title claims abstract description 136
- 238000000034 method Methods 0.000 title claims abstract description 48
- 238000012216 screening Methods 0.000 title claims abstract description 43
- 239000002689 soil Substances 0.000 claims abstract description 150
- 238000005065 mining Methods 0.000 claims abstract description 60
- 230000012010 growth Effects 0.000 claims abstract description 49
- 238000002386 leaching Methods 0.000 claims description 61
- 230000035784 germination Effects 0.000 claims description 52
- 239000013078 crystal Substances 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000012153 distilled water Substances 0.000 claims description 20
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 claims description 15
- 229930002875 chlorophyll Natural products 0.000 claims description 15
- 235000019804 chlorophyll Nutrition 0.000 claims description 15
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 15
- 230000035040 seed growth Effects 0.000 claims description 14
- 230000006378 damage Effects 0.000 claims description 12
- 208000027418 Wounds and injury Diseases 0.000 claims description 11
- 208000014674 injury Diseases 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 9
- 229940118019 malondialdehyde Drugs 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 213
- 239000004576 sand Substances 0.000 abstract description 6
- 244000025254 Cannabis sativa Species 0.000 abstract description 3
- 238000005070 sampling Methods 0.000 description 16
- 150000003839 salts Chemical class 0.000 description 12
- 239000010410 layer Substances 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 239000011780 sodium chloride Substances 0.000 description 10
- 239000000203 mixture Substances 0.000 description 6
- 239000003245 coal Substances 0.000 description 5
- -1 conductivity Chemical compound 0.000 description 4
- 238000003973 irrigation Methods 0.000 description 4
- 230000002262 irrigation Effects 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 239000002344 surface layer Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000234643 Festuca arundinacea Species 0.000 description 2
- 244000303040 Glycyrrhiza glabra Species 0.000 description 2
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 description 2
- 241000700189 Hystrix <Rodentia> Species 0.000 description 2
- 241000209082 Lolium Species 0.000 description 2
- 241000195474 Sargassum Species 0.000 description 2
- 241001145009 Sophora alopecuroides Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 description 1
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000010883 coal ash Substances 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000010881 fly ash Substances 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940010454 licorice Drugs 0.000 description 1
- 235000011477 liquorice Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The application provides a screening method of saline-alkali tolerant plants, relates to the field of ecological environment, and solves the problem that sand storm occurs frequently because the current severe saline-alkali causes the dead grass in a mining area. The screening method comprises the following steps: extracting saline-alkali components from a soil sample of a mining area; cultivating plants to be screened by utilizing the saline-alkali components to obtain growth data of the plants to be screened; and determining target plants from the plants to be screened according to the growth data. The screening method of the saline-alkali tolerant plants is used for screening out the plants suitable for being planted in the mining area.
Description
Technical Field
The application relates to the field of ecological environment, in particular to a screening method of saline-alkali tolerant plants.
Background
The Xinjiang gobi in China has a large number of open-air mining areas with large areas, and soil in the mining areas gradually becomes severe saline-alkali soil under the influence of climate and mining area wastes (coal ash, burnt stone, coal gangue and the like).
Severe saline and alkaline causes the grass to be nearly insanitary in a mining area, so that sand storm occurs frequently and great harm is brought to the ecological environment. Therefore, screening out saline-alkali tolerant plants suitable for planting in the mining area is of great importance to ecological restoration of the mining area.
Disclosure of Invention
The invention provides a screening method of saline-alkali tolerant plants, which can be used for solving the problem that sand storm occurs frequently because severe saline-alkali causes the grasses to be nearly insbesonderant in a mining area in the prior art.
The embodiment of the invention provides a screening method of saline-alkali tolerant plants, which comprises the following steps:
extracting saline-alkali components from a soil sample of a mining area;
cultivating plants to be screened by utilizing the saline-alkali components to obtain growth data of the plants to be screened;
and determining target plants from the plants to be screened according to the growth data.
Optionally, in one embodiment, the extracting saline alkali component from the mining area soil sample includes:
leaching the soil sample to obtain a leaching solution corresponding to the soil sample;
drying the leaching solution to obtain a crystal containing the saline-alkali component;
the method for cultivating the plants to be screened by utilizing the saline-alkali components comprises the following steps: preparing the crystal into a solution, and cultivating the plant to be screened by using the solution.
Optionally, in an embodiment, before leaching the soil sample to obtain a leaching solution corresponding to the soil sample, the screening method further includes:
screening the soil sample by using a screen with a preset aperture;
drying the soil sample passing through the screen;
the leaching of the soil sample to obtain a leaching solution corresponding to the soil sample comprises: and leaching the dried soil sample to obtain a leaching liquor corresponding to the soil sample.
Optionally, in an embodiment, the leaching the soil sample to obtain a leaching solution corresponding to the soil sample includes:
mixing the soil sample with distilled water to obtain a mixed sample;
and oscillating the mixed sample to obtain a leaching liquor corresponding to the soil sample.
Optionally, in one embodiment, the mixing the soil sample with distilled water to obtain a mixed sample comprises
Dividing the soil sample into at least two soil subsamples;
respectively mixing each soil subsample with distilled water to obtain a first mixed sample corresponding to each soil subsample;
the step of oscillating the mixed sample to obtain a leaching liquor corresponding to the soil sample comprises the following steps: and respectively oscillating the first mixed sample corresponding to each part of the soil subsample to obtain a first leaching solution corresponding to each part of the soil subsample, and combining the first leaching solutions corresponding to each part of the soil subsample to obtain a leaching solution corresponding to the soil sample.
Optionally, in an embodiment, the plant to be screened includes a seed corresponding to the plant to be screened, the growth data includes a seed growth indicator, and the seed growth indicator includes at least one of:
germination rate, germination index, germination vigor, injury rate and root-to-bud ratio.
Optionally, in one embodiment, the configuring the crystal into a solution, and the growing the plant to be screened with the solution comprises:
configuring the crystal into at least two concentrations of solutions as experimental culture solutions, wherein a preset concentration gradient exists between the at least two concentrations of solutions;
and cultivating seeds corresponding to the plants to be screened by using the experimental culture solution.
Optionally, in an embodiment, the plant to be screened includes a seedling plant corresponding to the plant to be screened at a seedling stage, the growth data includes a seedling stage physiological index, and the seedling stage physiological index includes at least one of:
malondialdehyde content, conductivity, chlorophyll content, Na+The content of,K+And (4) content.
Optionally, in one embodiment, the configuring the crystal into a solution, and the growing the plant to be screened with the solution comprises:
configuring the crystal into at least two concentrations of solutions as experimental culture solutions, wherein a preset concentration gradient exists between the at least two concentrations of solutions;
and culturing seedling plants corresponding to the plants to be screened in the seedling stage by using the experimental culture solution.
Optionally, in one embodiment, the plant to be screened comprises seeds corresponding to the plant to be screened and seedling plants corresponding to the plant to be screened at a seedling stage; the growth data comprises seed growth indexes and seedling stage physiological indexes;
determining a target plant from the plants to be screened according to the growth data, wherein the determining comprises the following steps: and determining a target plant from the plant to be screened according to the seed growth index and the seedling stage physiological index.
The beneficial effect that this application brought is as follows:
according to the screening method of the saline-alkali tolerant plants, provided by the embodiment of the application, saline-alkali components are extracted from a soil sample of a mining area; cultivating plants to be screened by utilizing the saline-alkali components to obtain growth data of the plants to be screened; determining target plants from the plants to be screened according to the growth data; can screen out saline-alkali tolerant plants, thereby restoring the ecological environment of a mining area by planting the screened saline-alkali tolerant plants in the mining area.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts. In the drawings:
FIG. 1 is a schematic flow chart of a method for screening saline-alkali tolerant plants according to an embodiment of the present application;
FIG. 2 is a schematic flow chart of another method for screening saline-alkali tolerant plants according to the embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the technical solutions of the present application will be described in detail and completely with reference to the following specific embodiments of the present application and the accompanying drawings. It should be apparent that the described embodiments are only some of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
As described in the background of the application, the number and area of the Xinjiang Gobi open pit coal mines are large, the mining areas belong to typical temperate continental arid climates, the drying is less rainy, the annual precipitation is only 33.8mm, and the annual evaporation capacity is as high as 3300 mm; meanwhile, the wastes such as fly ash, burnt stone and coal gangue in the mining area also occupy a large amount of land. Under the influence of climate and mining area wastes, most of the soil in the mining area is severe saline-alkali soil, the pH value is about 7-7.7, the total salt content is 8.53-16.6 g/kg, the content of various nutrients in the soil is low, particularly, nutrient elements such as nitrogen, phosphorus and the like are extremely deficient, the plant growth faces severe environmental conditions, the mining area is almost grass-grown, and then, the sand storm is frequent. The 'tree planting and sand control' is an economic means for restoring the ecological environment of a mining area at present, so that saline-alkali tolerant plants suitable for being planted in the mining area are screened out, and the screened saline-alkali tolerant plants are planted in the mining area and are of great importance for restoring the ecological environment of the mining area.
Aiming at the problems, the application provides a screening method of saline-alkali tolerant plants, which is used for solving the problem that sand storm occurs frequently because the current severe saline-alkali causes the dead grass in a mining area; as shown in fig. 1, the method may include the steps of:
step 101: saline-alkali components are extracted from a soil sample of a mining area.
Wherein, the saline alkali component can comprise chloride, sulfate, carbonate and the like. The saline-alkali component can be extracted from the soil sample, and the crystal can comprise not only the saline-alkali component in the soil sample, but also other components in the soil sample, such as mineral components and the like.
Before extracting the saline-alkali component from the mining area soil sample in step 101, the method may further include: collecting the soil sample. In practical application, a plurality of sampling points (such as 5-10) can be selected from a mining area according to the distribution condition of a saline-alkali layer of the soil in the mining area, the same depth is dug at each sampling point, and the soil of a surface layer, a shallow layer and a deep layer is collected at each sampling point; then, aiming at each sampling point, firstly mixing the collected surface layer soil, shallow layer soil and deep layer soil to obtain first mixed soil corresponding to the sampling point; and finally, mixing the first mixed soil corresponding to all sampling points to obtain a mining area soil sample. The mining area soil sample obtained by the sampling mode can accurately reflect the characteristics of the mining area soil.
Step 102: and cultivating the plants to be screened by utilizing the saline-alkali components, and acquiring growth data of the plants to be screened.
When the plant is cultivated by using the saline-alkali component, the saline-alkali component can be prepared into an experimental culture solution to cultivate the plant. When a crystal containing a salt-alkali component is extracted from soil, the crystal can be prepared into an experimental culture solution. In order to reduce the composition of each component (such as saline-alkali component and other components influencing plant growth) in the soil sample of the mining area as much as possible, the crystal can be prepared by distilled water when the crystal is prepared into an experimental culture solution.
The plant to be screened may include tall fescue, ryegrass, elymus, sargassum, licorice, sophora alopecuroides, etc. The number of the plants to be screened can include one or more, and when the number of the plants to be screened is one, the scheme provided by the embodiment of the application can also be regarded as that the saline-alkali tolerance of the plants is identified to determine whether the plants are suitable for being planted in the mining area.
In practical applications, the plant to be screened may be a seed corresponding to the plant to be screened and/or a seedling plant corresponding to the plant to be screened at a seedling stage.
For example, in an embodiment, the plant to be screened is a seed corresponding to the plant to be screened, and in this case, in step 102, the plant to be screened is cultivated by using the saline-alkali component, so as to obtain growth data of the plant to be screened, specifically, the plant to be screened is cultivated by using the saline-alkali component, so as to obtain growth data of the seed corresponding to the plant to be screened, which may also be referred to as a seed growth indicator. Wherein the seed growth index may include at least one of germination rate, germination index, germination vigor, injury rate, and root-to-shoot ratio.
It can be understood that, work as treat the screening plant does treat when screening the seed that the plant corresponds, can be right treat that the germination phase of screening plant carries out salt and alkali tolerance evaluation, can judge through germination percentage, germination index, vigor of germination, injury rate, root-bud ratio and cultivate under saline and alkali composition, treat the germination condition of the seed that the screening plant corresponds, and then can select the seed that suitable seeding is in the mining area according to the germination condition. In practical application, the germination condition of the seeds corresponding to the plant to be screened can be judged by simultaneously combining the germination rate, the germination index, the germination vigor, the injury rate and the root-bud ratio, and then the seeds suitable for being sown in a mining area can be accurately screened.
For example, in another embodiment, the plant to be screened is a seedling plant corresponding to the plant to be screened in a seedling stage, and in this case, in step 102, the saline-alkali component is used to cultivate the plant to be screened, so as to obtain growth data of the plant to be screened, specifically, the saline-alkali component is used to cultivate the seedling plant corresponding to the plant to be screened in the seedling stage, so as to obtain growth data of the seedling plant corresponding to the plant to be screened in the seedling stage, which may also be referred to as a seedling stage physiological index. Wherein the physiological index of seedling stage comprises malondialdehyde content, conductivity, chlorophyll content, and Na+Content and K+At least one of the contents.
It is understood that when the plant to be screened is a seedling plant corresponding to the plant to be screened at a seedling stage, the plant to be screened may be screenedEvaluating the saline-alkali resistance of the plant in the seedling stage according to the content of malonaldehyde, the conductivity, the chlorophyll content and Na+Content, K+The content can be judged under saline-alkali component is cultivated, the growth condition of the seedling plant corresponding to the plant to be screened in the seedling stage can be further screened out, and the plant seedling plant suitable for being transplanted to a mining area for planting can be further screened out according to the growth condition of the seedling plant. In practical application, the malonaldehyde content, the conductivity, the chlorophyll content and the Na can be combined at the same time+Content and K+The growth condition of the seedling plant corresponding to the plant to be screened in the seedling stage is judged according to the content, and then the plant seedling plant suitable for being transplanted to a mining area for planting can be accurately screened.
In a more preferred embodiment, the plant to be screened includes a seed corresponding to the plant to be screened and a seedling plant corresponding to the plant to be screened in a seedling stage, and in this case, in step 102, the plant to be screened is cultivated by using the saline-alkali component to obtain growth data of the plant to be screened, specifically, the seed corresponding to the plant to be screened and the seedling plant corresponding to the plant to be screened in the seedling stage are cultivated by using the saline-alkali component to obtain a seed growth index and a seedling stage physiological index. Wherein the seed growth index may include at least one of germination percentage, germination index, germination potential, injury rate and root-to-shoot ratio, and the seedling stage physiological index may include malondialdehyde content, conductivity, chlorophyll content, Na+Content and K+At least one of the contents.
It can be understood that when the plant to be screened includes the seed that the plant to be screened corresponds with when the plant to be screened is in the seedling that seedling stage corresponds, can be right the germination phase and seedling stage that wait to screen the plant carry out salt and alkali tolerance evaluation respectively, can judge through the seed growth index of plant germination phase and the seedling stage physiological index of seedling stage and cultivate under salt and alkali composition cultivates, wait to screen the plant and be in the growth condition of germination phase and seedling stage, and then can select from the germination phase to the seedling stage all can carry out the plant of planting in the mining area.
Step 103: and determining target plants from the plants to be screened according to the growth data.
Wherein the target plant is a plant suitable for being planted in a mining area, namely a saline-alkali tolerant plant.
According to the growth data, plants with better growth vigor under the cultivation of saline-alkali components can be screened out, and then the plants with better growth vigor can be determined as plants suitable for being planted in the mining area.
When the plant to be screened is the seed corresponding to the plant to be screened, the germination rate, the germination index, the germination vigor, the injury rate and the root-bud ratio can be used as different seed growth indexes, and the saline-alkali tolerance score of each seed under each growth index is obtained: and U (Xij) ═ Xijmin)/(Xjmax-Xjmin), finally, aiming at each seed, adding the salt and alkali tolerance scores under different growth indexes to obtain the salt and alkali tolerance score of the seed, ranking various seeds according to the salt and alkali tolerance score, and determining the salt and alkali tolerance seed according to the ranking. For example, the first or top ranked seed is determined to be a salt tolerant seed. Wherein U (Xij) in the formula represents the salt and alkali tolerance score value of the growth index of i plant j; xij represents the corresponding value of i plant j growth index; xjmax and Xjmin represent the corresponding maximum and minimum values of the j growth index for i plants.
When the plant to be screened is a seedling plant corresponding to the seedling stage of the plant to be screened, the malondialdehyde content, the conductivity, the chlorophyll content and the relative Na content can be adjusted+/K+The content is used as different seedling stage physiological indexes, and the salt and alkali tolerance score of each seed under each physiological index is obtained: and U (Xij) ═ Xijmin)/(Xjmax-Xjmin), finally, adding the saline-alkali tolerance scores under different physiological indexes aiming at each seed to obtain the saline-alkali tolerance score of the seedling, ranking various seedling plants according to the saline-alkali tolerance score, and determining the saline-alkali tolerance seedling plants according to the ranking. For example, the first or top ranked plant is identified as a saline tolerant seedling plant. Wherein, U (Xij) represents the saline-alkali tolerance score value of the physiological index of i plant j; xij represents the corresponding value of the physiological index of i plant j; xjmax and Xjmin represent the corresponding maximum and minimum values of the physiological index of j for i plants.
When the plant to be screened comprises the seed corresponding to the plant to be screened and the seedling plant corresponding to the plant to be screened in the seedling stage, the saline-alkali tolerance scores under different growth indexes and the saline-alkali tolerance scores under different physiological indexes can be added for each plant, the saline-alkali tolerance score of the plant is finally obtained, various plants are ranked according to the saline-alkali tolerance scores, and the saline-alkali tolerance plant can be determined according to the ranking.
It can be understood that, by adopting the screening method of saline-alkali tolerant plants provided by the embodiment of the application, the plants to be screened are cultivated by utilizing saline-alkali components extracted from the soil samples of the mining area, the growth data of the plants to be screened are obtained, the target plants are determined from the plants to be screened according to the growth data, the saline-alkali tolerant plants can be screened, and therefore, the ecological environment of the mining area can be recovered by planting the screened saline-alkali tolerant plants in the mining area.
On the other hand, the embodiment of the application directly utilizes the saline-alkali components extracted from the mining area soil sample to cultivate the plants, and really reduces the influence of the mining area soil on the growth of the plants, so that saline-alkali tolerant plants suitable for being planted in the mining area can be accurately screened out.
In order to successfully extract the saline-alkali component from the mining area soil sample, in one embodiment, the step 101 of extracting the saline-alkali component from the mining area soil sample specifically comprises: leaching the soil sample to obtain a leaching solution corresponding to the soil sample; and drying the leaching liquor to obtain a crystal containing the saline-alkali component. Then step 102, cultivating the plant to be screened by using the saline-alkali component, which specifically comprises: preparing the crystal into a solution, and cultivating the plant to be screened by using the solution.
In order to prevent other substances introduced into the solution from affecting the growth of plants and causing inaccurate screening of saline-alkali tolerant plants, in one embodiment, the soil sample is leached to obtain a leaching solution corresponding to the soil sample, and the leaching solution specifically comprises: mixing the soil sample with distilled water to obtain a mixed sample; and oscillating the mixed sample to obtain a leaching liquor corresponding to the soil sample.
It can be understood that the saline-alkali component is generally water-soluble saline-alkali, and the mixed sample is prepared by using distilled water and is oscillated, on one hand, the saline-alkali component in the soil sample is dissolved in the distilled water, and then solid soil can be separated and removed by standing, centrifuging and the like, and a leaching solution containing various components in the soil sample is reserved, wherein the leaching solution contains the saline-alkali component in the soil sample. On the other hand, the mixed sample is prepared by using distilled water, so that the influence on the growth of plants caused by introducing other substances into the mixed sample can be avoided. In practical application, after the leaching liquor corresponding to the soil sample is obtained, the pH value and the total saline-alkali content of the leaching liquor can be further determined.
In order to further accelerate the obtaining process of the leaching liquor corresponding to the soil sample, in one embodiment, the soil sample is mixed with distilled water to obtain a mixed sample, which specifically includes: dividing the soil sample into at least two soil subsamples; and respectively mixing each soil subsample with distilled water to obtain a first mixed sample corresponding to each soil subsample. Then, the oscillating the mixed sample to obtain a leaching solution corresponding to the soil sample includes: and respectively oscillating the first mixed sample corresponding to each part of the soil subsample to obtain a first leaching solution corresponding to each part of the soil subsample, and combining the first leaching solutions corresponding to each part of the soil subsample to obtain a leaching solution corresponding to the soil sample.
The soil sample is divided into at least two soil subsamples, wherein the soil sample can be divided into 5-10 parts, and each part is one soil subsample. The mass ratio of each soil subsample to the distilled water may be 3: 1-8: 1; the time period for oscillating each first mixed sample can be 2-5 minutes.
It can be understood that by dividing the soil sample into at least two soil subsamples and leaching each soil subsample separately, the leaching process of multiple soil subsamples can be performed simultaneously, and then the leaching time of the soil sample can be shortened, so that the saline-alkali component in the soil sample can be extracted quickly.
In practice, other impurities, such as stones, may be present in the soil sample collected from the mine site, and the soil sample may contain some moisture, which may affect the subsequent leaching process. Therefore, in an embodiment, before leaching the soil sample to obtain a leaching solution corresponding to the soil sample, the method further includes: screening the soil sample by using a screen with a preset aperture; and drying the soil sample passing through the screen. Then, leaching the soil sample to obtain a leaching solution corresponding to the soil sample, specifically including: and leaching the dried soil sample to obtain a leaching liquor corresponding to the soil sample. Wherein the predetermined aperture may be 1 mm.
In order to further accurately screen saline-alkali tolerant plants suitable for planting in a mining area, when the crystals are prepared into a solution to cultivate the plants to be screened, the crystals can be prepared into solutions with various concentrations as an experimental culture solution (the plants cultivated by the experimental culture solution can be called an experimental group), and the solutions with various concentrations have preset concentration gradients. The multiple solutions are used for cultivating each plant in the plants to be screened, and in order to further increase the screening accuracy, the number of plants cultivated in each concentration is multiple for each plant in the plants to be screened; a control group can also be set, and distilled water is cultured as the control group.
The crystal can be prepared into solutions with 3-8 concentrations, the preset concentration gradient between the solutions can be 0.5% -1.0%, each plant to be screened can comprise 3-5 groups of plants cultivated in each concentration, and the number of the plants in each group can be the same.
When the plant to be screened is the corresponding seed, the crystal is configured into 3-8 solutions with concentration gradient difference of 0.5% -1.0%, and a distilled water culture control group is arranged. Aiming at each plant in the plants to be screened, each concentration of cultivated plants comprises 3-5 groups, and each group comprises 50 or 100 seeds. The cultivation process can be as follows: 50 or 100 seeds are placed in each culture dish, the solution prepared by the crystallisate is arranged in the culture dish, and the illumination and temperature environment for the growth of the seeds is that the illumination cycle is 12h, the day temperature is 25-30 ℃, the night cycle is 12h and the night temperature is 15-20 ℃. In order to ensure the constant concentration of saline alkali in the solution for cultivating seeds, a weighing method can be adopted to supplement the volatile water in time every day; the number of seed shoots can be recorded every 24h starting on day 2, and the experiment is ended 15 days after which the root length and germ length of the seeds are measured. In order to eliminate the difference between the experimental groups and the difference between the experimental group and the control group, the measured germination percentage, germination index, germination vigor, injury rate and root-to-bud ratio were converted into relative values, respectively. Specifically, the relative germination rate is (germination rate of experimental group/germination rate of control group) × 100%; the relative germination index is an experimental group (Sigma GT/DT)/a control group (Sigma GT/DT), wherein GT represents the germination rate of seeds on the Tth day, and DT represents the corresponding germination days; relative germination potential ═ (number of sprouts in experimental group/n × 100%)/(number of sprouts in control group/n × 100%), where n is the number of seeds; injury rate ═ [ (control group germination rate-experimental group germination rate)/control group germination rate ] × 100%; relative root-shoot ratio (experimental radicle length/experimental radicle length)/(control radicle length/control plantle length).
When the plant to be screened is a seedling plant corresponding to the seedling stage, the crystal is prepared into solutions with 3-8 concentrations, the difference between concentration gradients is 0.5% -1.0%, and a distilled water culture control group is arranged. Adopting a saline-alkali solution irrigation mode for pot cultivation, and placing 0.5-2.0 kg of culture soil (vermiculite: flower soil is 1:2) in each pot; the room temperature is maintained at 15-30 ℃, and natural lighting is performed. Aiming at each plant in the plants to be screened, each plant with the concentration corresponding to be cultivated comprises 3-5 groups (pots), 2-5 seeds are broadcast in each pot, thinning is carried out after sprouting and growing for 3 weeks, seedlings with good growth and equal quantity are left, saline-alkali solution with the corresponding concentration is irrigated after growing for a certain time (2-3 weeks), wherein the plants to be screened normally sprout and grow before thinning, and the saline-alkali solution is not irrigated. Sampling and testing malondialdehyde, conductivity, chlorophyll and Na of the plant sample after one week of irrigation+And K+Content (c); to eliminate the differences between experimental groups and between experimental and control groups, malondialdehyde, conductivity, chlorophyll, Na, were measured+And K+Content (wt.)They are converted into relative values, respectively. Specifically, the relative malonaldehyde content (experimental malonaldehyde content/control malonaldehyde content) × 100%; relative conductivity is experiment group conductivity/control group conductivity; relative chlorophyll content ═ experimental group chlorophyll content/control group chlorophyll content; relative to K+/Na+content-Experimental group K+/Na+Content/control group K+/Na+And (4) content.
Based on the method for screening saline-alkali tolerant plants provided in the above examples of the present application, a more specific example is also provided to illustrate the method for screening saline-alkali tolerant plants provided in the above examples of the present application, and it should be understood that this example is only an example and does not represent a limitation on the method for screening saline-alkali tolerant plants provided in the examples of the present application.
The implementation place is as follows: a coal mine area in hami city of hami coal mine area in Xinjiang. The mining area belongs to a typical continental climate, the annual average temperature is 9.8 ℃, the extreme maximum temperature is 43 ℃, and the extreme minimum temperature is-32 ℃; annual precipitation amount is 33.8mm, annual evaporation amount is 3300mm, annual average sunshine is 3358 hours, and frost-free period is 182 days; the surface layer of the soil is provided with a 10-20 cm saline-alkali layer.
The screening method of the saline-alkali tolerant plants comprises the following specific steps:
the plant species to be screened: tall fescue, ryegrass, elymus, sargassum, liquorice and sophora alopecuroides.
Step 201: and collecting a soil sample of the mining area. According to mining area soil salt and alkali layer distribution situation, select 6 typical sampling points, the same degree of depth is dug at every sampling point, and all gather the top layer at every sampling point, shallow layer and deep soil, then, to every sampling point, the top layer that obtains earlier the collection, shallow layer and deep soil mix, obtain the first mixed soil of this sampling point, and then obtain the first mixed soil that 6 typical sampling points correspond respectively, every first mixed soil weight 10 kilograms, mix the first mixed soil misce bene that corresponds 6 typical sampling points respectively and obtain mining area soil sample.
Step 202: and extracting saline-alkali components from the soil sample. Screening the soil sample through a screen with 1mm sieve pores, drying the soil sample passing through the sieve pores, dividing the soil sample into 5 parts of soil subsamples, each part of the soil subsamples is 1 kilogram, oscillating the soil subsamples on an oscillator for 3min according to the water-soil ratio of 5:1, and leaching water-soluble saline-alkali; combining leaching liquor of 5 soil subsamples, and determining the pH value and the total saline-alkali content of the leaching liquor; then drying the water in the leaching liquor to obtain a crystal.
Step 203: and cultivating seeds corresponding to the plants to be screened by utilizing the saline-alkali components to obtain growth indexes of the seeds corresponding to the plants to be screened. The crystals were prepared as solutions having 4 concentrations of 0.5%, 1.0%, 1.5% and 2.0%, respectively, with a concentration gradient of 0.5%, and a control group was incubated with distilled water. For each plant in the plants to be screened, the plants grown at each concentration included 3 groups, each group including 50 seeds. The cultivation process is as follows: 50 seeds were placed in each dish and germinated in a light cycle of 12 hours, at a day temperature of 26 ℃, a night cycle of 12 hours and at a night temperature of 15 ℃. The distilled water of the volatile water in the day is supplemented in time by a weighing method every day; the test was recorded starting on day 2 and the number of seed shoots was recorded every 24 h. The experiment was terminated in 15 days, and the length of the germ of the seeds were measured. Respectively converting the measured growth indexes, namely the germination rate, the germination index, the germination potential, the injury rate and the root-bud ratio into relative values, namely the relative index value of a certain growth index is (the index value of an experimental group/the index value of a control group) multiplied by 100%; for a specific conversion formula, reference may be made to the above embodiments, which are not described herein again.
Step 204: and culturing seedling plants corresponding to the plants to be screened in the seedling stage by utilizing the saline-alkali components, and acquiring the physiological indexes of the seedling plants corresponding to the plants to be screened in the seedling stage. The crystals were prepared as solutions having 4 concentrations of 0.5%, 1.0%, 1.5% and 2.0%, respectively, with a concentration gradient of 0.5%, and a control group was incubated with distilled water. Adopting a saline alkali solution irrigation treatment mode of pot cultivation; 2.0kg of culture soil (vermiculite: flower soil is 1:2) is placed in each pot; the room temperature is maintained at 15-30 ℃, and natural lighting is carried out; aiming at each plant in the plants to be screened, each plant with the corresponding concentration comprises 3 groups (pots), 2-5 seeds are broadcast in each pot, and the seeds grow in a germination mode by 3Thinning the seedlings after week (normally sprouting and growing the plants to be screened before thinning, irrigating without saline-alkali solution), leaving the seedlings with good growth and equal quantity, and irrigating with saline-alkali solution with corresponding concentration after 2 weeks of growth. Sampling and testing physiological indexes such as malondialdehyde, conductivity, chlorophyll and Na of the plant sample after one week of irrigation+And K+Content (c); the measured malondialdehyde, conductivity, chlorophyll and Na+And K+The contents are respectively converted into relative values, namely the relative index value of a certain physiological index is equal to (index value of an experimental group/index value of a control group) multiplied by 100 percent; for a specific conversion formula, reference may be made to the above embodiments, which are not described herein again.
Step 205: and determining a target plant from the plants to be screened according to the growth index and the physiological index. Namely, the saline-alkali tolerance of the plants to be screened in the germination stage and the seedling stage is comprehensively evaluated, and the relative index values (relative germination percentage, relative germination index, relative germination vigor, relative root-bud ratio and relative injury rate) of 5 growth indexes in the germination stage of the plants to be screened and the relative index values (relative malonaldehyde content, relative conductivity, relative chlorophyll content and relative K) of 4 physiological indexes in the seedling stage of the plants to be screened are compared+/Na+Content) is calculated by a membership function, and the saline alkali tolerance score of each plant to be screened is obtained: u (Xij) ═ Xijmin)/(Xjmax-Xjmin), and determining the saline-alkali tolerance degree of each plant to be screened according to the ranking; the saline alkali tolerance score of each plant to be screened is shown in table 1.
TABLE 1 saline alkali tolerance score of plants to be screened
The six plants are planted in the mining area, and the saline-alkali tolerance performance of the six plants is basically consistent with the ranking condition in the table 1, so that the saline-alkali tolerance plant screening method provided by the embodiment of the application can accurately identify the saline-alkali tolerance of the plants and accurately screen the plants suitable for being planted in the mining area.
It can be understood that the saline-alkali components in the mining area soil sample are extracted, and the plants to be screened are cultivated by the saline-alkali components, so that the saline-alkali structure and composition of the original mining area soil can be embodied, and the reliability of the screening result can be guaranteed. Meanwhile, the plants to be screened are respectively cultivated in the germination stage and the seedling stage, and relevant growth indexes and physiological indexes are determined, so that the saline-alkali tolerant plants are screened by adopting comprehensive indexes on the basis, and the accuracy of the screening result is further improved.
It should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A screening method for saline-alkali tolerant plants, which is characterized by comprising the following steps:
extracting saline-alkali components from a soil sample of a mining area;
cultivating plants to be screened by utilizing the saline-alkali components to obtain growth data of the plants to be screened;
and determining target plants from the plants to be screened according to the growth data.
2. The method for screening saline-alkali tolerant plants according to claim 1, wherein the extraction of saline-alkali components from the sample of the soil in the mining area comprises:
leaching the soil sample to obtain a leaching solution corresponding to the soil sample;
drying the leaching solution to obtain a crystal containing the saline-alkali component;
the method for cultivating the plants to be screened by utilizing the saline-alkali components comprises the following steps: preparing the crystal into a solution, and cultivating the plant to be screened by using the solution.
3. The method for screening saline-alkali tolerant plants according to claim 2, wherein before leaching the soil sample to obtain a leaching solution corresponding to the soil sample, the method further comprises:
screening the soil sample by using a screen with a preset aperture;
drying the soil sample passing through the screen;
the leaching of the soil sample to obtain a leaching solution corresponding to the soil sample comprises: and leaching the dried soil sample to obtain a leaching liquor corresponding to the soil sample.
4. The method for screening saline-alkali tolerant plants according to claim 2, wherein the leaching of the soil sample to obtain a leaching solution corresponding to the soil sample comprises:
mixing the soil sample with distilled water to obtain a mixed sample;
and oscillating the mixed sample to obtain a leaching liquor corresponding to the soil sample.
5. The method for screening saline-alkali tolerant plants according to claim 4, wherein the mixing the soil sample with distilled water to obtain a mixed sample comprises
Dividing the soil sample into at least two soil subsamples;
respectively mixing each soil subsample with distilled water to obtain a first mixed sample corresponding to each soil subsample;
the step of oscillating the mixed sample to obtain a leaching liquor corresponding to the soil sample comprises the following steps: and respectively oscillating the first mixed sample corresponding to each part of the soil subsample to obtain a first leaching solution corresponding to each part of the soil subsample, and combining the first leaching solutions corresponding to each part of the soil subsample to obtain a leaching solution corresponding to the soil sample.
6. The method for screening saline-alkali tolerant plants according to claim 2, wherein the plant to be screened comprises seeds corresponding to the plant to be screened, the growth data comprises seed growth indicators, and the seed growth indicators comprise at least one of the following:
germination rate, germination index, germination vigor, injury rate and root-to-bud ratio.
7. The method for screening saline-alkali tolerant plants according to claim 6, wherein the preparing the crystal into a solution, and the cultivating the plant to be screened with the solution comprises:
configuring the crystal into at least two concentrations of solutions as experimental culture solutions, wherein a preset concentration gradient exists between the at least two concentrations of solutions;
and cultivating seeds corresponding to the plants to be screened by using the experimental culture solution.
8. The method for screening the saline-alkali tolerant plant according to claim 2, wherein the plant to be screened comprises a seedling plant corresponding to the plant to be screened at a seedling stage, the growth data comprises seedling stage physiological indexes, and the seedling stage physiological indexes comprise at least one of the following:
malondialdehyde content, conductivity, chlorophyll content, Na+Content, K+And (4) content.
9. The method for screening saline-alkali tolerant plants according to claim 8, wherein the configuring the crystal into a solution, and the cultivating the plant to be screened with the solution comprises:
configuring the crystal into at least two concentrations of solutions as experimental culture solutions, wherein a preset concentration gradient exists between the at least two concentrations of solutions;
and culturing seedling plants corresponding to the plants to be screened in the seedling stage by using the experimental culture solution.
10. The method for screening the saline-alkali tolerant plant according to claim 2, wherein the plant to be screened comprises seeds corresponding to the plant to be screened and seedling plants corresponding to the plant to be screened at a seedling stage; the growth data comprises seed growth indexes and seedling stage physiological indexes;
determining a target plant from the plants to be screened according to the growth data, wherein the determining comprises the following steps: and determining a target plant from the plant to be screened according to the seed growth index and the seedling stage physiological index.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111234042.8A CN113951135A (en) | 2021-10-22 | 2021-10-22 | Screening method of saline-alkali tolerant plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111234042.8A CN113951135A (en) | 2021-10-22 | 2021-10-22 | Screening method of saline-alkali tolerant plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113951135A true CN113951135A (en) | 2022-01-21 |
Family
ID=79466500
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111234042.8A Pending CN113951135A (en) | 2021-10-22 | 2021-10-22 | Screening method of saline-alkali tolerant plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113951135A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067206A (en) * | 2022-07-22 | 2022-09-20 | 山西农业大学园艺学院 | Screening method and screening device for saline-alkali tolerant garden ground cover plants |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785383A (en) * | 2010-03-17 | 2010-07-28 | 吉林省农业科学院 | Method for screening corn salt/alkali-tolerance germplasm |
| CN103828713A (en) * | 2014-02-25 | 2014-06-04 | 江苏沿海地区农业科学研究所 | Saline-alkaline tolerant germplasm screening and high-yield cultivating method of barley |
| CN110140602A (en) * | 2019-06-14 | 2019-08-20 | 国家能源投资集团有限责任公司 | The method of arid biogeographic zone coal field revegetation |
| AU2020101605A4 (en) * | 2020-07-31 | 2020-09-17 | Beijing Normal University | A Fluid Seed Formula, Fluid Seed And Manufacturing Process Thereof Used For Vegetation Restoration of the Tidal Salt-Marsh In Coastal Wetland |
| CN112273087A (en) * | 2020-10-30 | 2021-01-29 | 黑龙江八一农垦大学 | Method for identifying saline-alkali resistance of soybeans |
-
2021
- 2021-10-22 CN CN202111234042.8A patent/CN113951135A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101785383A (en) * | 2010-03-17 | 2010-07-28 | 吉林省农业科学院 | Method for screening corn salt/alkali-tolerance germplasm |
| CN103828713A (en) * | 2014-02-25 | 2014-06-04 | 江苏沿海地区农业科学研究所 | Saline-alkaline tolerant germplasm screening and high-yield cultivating method of barley |
| CN110140602A (en) * | 2019-06-14 | 2019-08-20 | 国家能源投资集团有限责任公司 | The method of arid biogeographic zone coal field revegetation |
| AU2020101605A4 (en) * | 2020-07-31 | 2020-09-17 | Beijing Normal University | A Fluid Seed Formula, Fluid Seed And Manufacturing Process Thereof Used For Vegetation Restoration of the Tidal Salt-Marsh In Coastal Wetland |
| CN112273087A (en) * | 2020-10-30 | 2021-01-29 | 黑龙江八一农垦大学 | Method for identifying saline-alkali resistance of soybeans |
Non-Patent Citations (4)
| Title |
|---|
| 何淼等: "模拟复合盐碱胁迫对芒幼苗生理特性的影响", 《草业科学》, no. 07, 8 July 2016 (2016-07-08), pages 1342 - 1352 * |
| 王雁等: "四种草坪草抗盐能力的研究", 《中国城市林业》, no. 6, 15 December 2004 (2004-12-15), pages 36 - 38 * |
| 申午艳等: "盐碱胁迫下黑麦草生长及离子微区分布特征", 《草业学报》, no. 02, 20 February 2020 (2020-02-20), pages 52 - 63 * |
| 穆平: "《作物育种学》", 31 July 2017, pages: 172 - 173 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115067206A (en) * | 2022-07-22 | 2022-09-20 | 山西农业大学园艺学院 | Screening method and screening device for saline-alkali tolerant garden ground cover plants |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102090245B (en) | Cultivation method for plant Maca | |
| CN103262724B (en) | Camellia oleifera forest ecological conservation composite cultivation mode | |
| Xing et al. | Comparison of yield traits in rice among three mechanized planting methods in a rice-wheat rotation system | |
| CN104186189B (en) | A kind of mulberry tree point disk seedling growing method | |
| CN102487676A (en) | Method of planting herba houttuyniae in greenhouse | |
| CN107969291B (en) | Landscape grass tip circular cultivation method | |
| CN101946602A (en) | Method for culturing and transplanting seedling of cassava | |
| CN107771604A (en) | A kind of efficient cultivation method of oil tea | |
| KR102049746B1 (en) | Method for cultivating high quality Astragalus membranaceus plug seedling using nutrition solution having controlled electrical conductivity | |
| CN109047304B (en) | Screening method and application of rhizomes plants for repairing soil uranium and cadmium combined pollution | |
| CN109006220A (en) | Method suitable for the plantation of S.plumbizincicola large area | |
| CN101983550A (en) | Artificial domestication, planting and cultivation method of wild calotropis gigantea | |
| CN114258813A (en) | Multilayer forest construction method suitable for eucalyptus forest reconstruction | |
| CN113951135A (en) | Screening method of saline-alkali tolerant plants | |
| CN112544381A (en) | Method for planting bletilla striata in karst arbor forest land | |
| CN109526606B (en) | Method for inducing open field natural vegetation to quickly recover | |
| CN113994866A (en) | Method for improving rice seedling raising soil by artificial humus | |
| CN102318530A (en) | Labor-saving walnut planting method suitable for mechanical operation | |
| CN106212189A (en) | A kind of method that Arundo donax is planted at saline and alkaline desert Land Size | |
| CN111631065A (en) | Economic planting method under southern low-efficiency artificial forest | |
| CN110972863A (en) | Method for breeding sweet potato seedlings for vegetables in winter greenhouse | |
| CN109832102A (en) | A method of utilizing plant in treating sand field | |
| CN108633369B (en) | Method for repairing degraded soil in southwest Asia alpine region by utilizing nitrogen-fixing tree species drynaria | |
| CN109287398A (en) | A kind of mind green pepper No.1 high yield cultivating method | |
| CN110447444B (en) | Method for artificially promoting natural renewal of dalbergia odorifera |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220121 |