AU2020101605A4 - A Fluid Seed Formula, Fluid Seed And Manufacturing Process Thereof Used For Vegetation Restoration of the Tidal Salt-Marsh In Coastal Wetland - Google Patents

Abstract

The invention relates to a fluid seed formula, fluid seed and manufacturing process thereof used for vegetation restoration of the Tidal Salt-marsh in coastal wetland. In particular, the present invention provides a fluid seed formulation that includes the following components. water-retaining agent, auxiliary adhesive, nutrition promoter. Hydroxyethyl alcohol is selected as water retaining agent and mainly used to store water. The auxiliary binder is composed of coastal clay, attapulgite clay and hydroxyethyl cellulose, which can resist tidal hydrodynamic disturbance by bonding with surface soil. Nutritional promoters include biomass particles, chito-oligosaccharides, and polyasparagine acid, tea saponin, indole acetic acid, humus, fermented faeces and oysters shell powder, which plays the role of nutrition and growth regulation. The present invention provides a fluid seed that solves the problems of uneven seed flow diffusion and non-effective planting in tide beach of coastal wetland, and improved the seed emergence rate. In addition, that invention also provide a manufacture process of a liquid seed, which is simple, low cost, and suitable for mass production. 100 -- Oppt -- 5ppt -A 1Qppt 80 - l5ppt 20ppt _ S60 S40 _ _ (D 20 0 1 0 2 4 6 8 10 Time Fig.1I

Description

100 -- Oppt -- 5ppt -A 1Qppt

80 - l5ppt 20ppt _

S60

S40 _ _

(D 20

0 1

0 2 4 6 8 10 Time

Fig.1I

AUSTRALIA

PATENTS ACT 1990

PATENT SPECIFICATION FOR THE INVENTION ENTITLED:

A Fluid Seed Formula, Fluid Seed And Manufacturing Process Thereof Used For

Vegetation Restoration of the Tidal Salt-Marsh In Coastal Wetland

The invention is described in the following statement: -

A Fluid Seed Formula, Fluid Seed And Manufacturing Process Thereof Used

For Vegetation Restoration of the Tidal Salt-Marsh In Coastal Wetland

TECHNICAL FIELD

[0001] The present invention relates to a fluid seed formula, fluid seed and its preparation

process for vegetation restoration of tidal flat salt marshes in coastal wetlands.

BACKGROUND

[0002] Wetlands are one of the most important ecosystems in the world, and they are also

the ecosystems rich in biodiversity and high productivity in nature, so they are called the

"source of life." Compared with other types of wetlands, coastal wetlands are characterized

much by rich biodiversity and high productivity, and they are the functional blocks with

special natural conditions, complex ecosystems and most economic significance in the

coastal zone. However, the integrity of the structure, function and process integrity of

coastal wetland ecosystem are seriously damaged and the wetland area is seriously shrunk

under the dual action of human activities and climate change.

[0003] China and the rest of the world have made unremitting efforts in coastal wetland

restoration, and many coastal countries have large-scale coastal restoration plans. The

repair technologies involved can be divided into four categories according to the repair

objects. First, substrate restoration technology includes wetland substrate reconstruction

technology, wetland and soil erosion control technology, and desilting technology; Second,

the hydrological condition restoration technology includes the restoration technology of

hydrological conditions (removing dams, building approach channels and other water conservancy projects) and the water environment quality improvement technology

(sewage treatment technology, and the eutrophication control technology of water bodies);

Third, soil remediation technology includes soil pollution control technology and soil

fertility restoration technology; Fourth, vegetation restoration technology includes

transplanting and propagation plant technology, soil seed bank technology and invasive

species removal technology. However, most of that repair technology can not solve the

problem of uneven distribution of the seed bank due to the disturbance of the

hydrodynamic force and the problems of water, salt and nutrition stress existing in the cut

off area of the see. In that actual implementation process, the seedling raising success rate

is often less than 10 percent. Therefore, there is an urgent need for a new recovery method

with enhanced seeds as the core, which may be the key to resisting hydrodynamic

interference, alleviating water and salt stress, improving seedling establishment, plant

growth and natural seed recovery. Most of the seeds are collected from wild sources,

representing limited resources not to be wasted. At present, there are more and more

researches on ecological restoration of degraded land by enhancing vegetation seeds, but

the related technologies are applied to degraded pastures in areas affected by wildfires,

semi-arid arid areas and degraded land after mining. There is no related technology in the

field of ecological restoration of coastal wetlands research and reports.

[0004] Under the unique environment of coastal wetlands, tidal events play an important

role in the transport and distribution of vegetation seeds, and the intensity and density of

hydrological connectivity determine the structure of soil seed banks and the seed retention

capacity. Strong winds and tidal events in the fall and winter of each year transport the

seeds scattered in the mid-low tide zone to the tide zone, which greatly reduced the utilization rate of seed banks in the ecosystem. The high salinity and low water content of topsoil during the spring seed germination window greatly inhibited the seed germination rate. The present invention belongs to the field of biology and ecology, and from the point of view of enhancing vegetation seeds, in view of the special tide phenomenon and soil salinization in the tide beach of coastal wetland, which leads to the degradation of vegetation in coastal wetland, making fluid seeds that are wave-proof, water-retaining and salt-tolerant can effectively improve the damage and interference of tidal events to soil seed banks and germinating seedlings, and can cope with the inhibition of spring drought to seed germination. It provides a new way for the restoration of degraded vegetation in tidal flat of coastal wetland.

[0005] At present, the fluid seed technology is mainly applied to the agricultural field, and

in the related technology, there are a large number of organic or inorganic chemical

substances in the active ingredients, such as insecticides, fungicides and herbicides, etc.

The cost is high and will cause environmental pollution, unable to meet the large-scale and

pollution-free requirements of ecological restoration. In addition, the special tidal

environment, soil salinization and spring drought in the tidal flat of the coastal wetland are

also a test to the fluid seed technology.

[0006] The present invention is hereby presented.

SUMMARY

[0007] An object of the present invention is to provide a fluid seed formulation, fluid seed

and its manufacturing process for wave resistance and drought resistance of a coastal

wetland, in view of the existing problem of vegetation degradation of the tidal flat of the

coastal wetland. The fluid seed formula has the advantages of pertinence and universality, and has the merits of wave prevention, drought resistance, salt and alkali resistance and nutrition; meanwhile, the raw material components are mostly pure natural components, which are environment-friendly and pollution-free, meeting the requirements of ecological restoration of coastal wetlands. The fluid seeds are slowly combined with the soil under the action of the auxiliary binder after being sprayed to the tidal flat, thereby effectively reducing the damage and disturbance of the soil seed bank caused by the tidal event. And the water-retaining agent therein will absorb water and swell after each tide comes, thereby forming a series of "mini reservoirs" in the operation area, providing water for seed germination and seedling growth, at the same time, it reduced the salinity of soil around the area, promoted the germination of seeds, and significantly increased the survival rate of seeds and seedlings. The preparation method of the fluid seed is simple in operation and low in cost, and is suitable for popularization in a large area.

[0008] In order to achieve the above object of the present invention, the following

technical solution is adopted:

[0009] The present invention provides a fluid seed formulation for salt marsh vegetation

restoration in coastal wetlands, comprising a water retaining agent, an auxiliary binder,

and a nutrition promoter

[0010] The weight ratio of the water-retaining agent, the auxiliary binder and the

nutrition promoter is 1-5: 10-20: 1 to 5.

[0011] The water retaining agent above is hydroxyethyl cellulose;

[0012] The auxiliary binders described above include coastal clay, attapulgite clay and

hydroxyethyl cellulose.

[0013] In particular, the ratio by weight is 30-40: 40-60: 2-5.

[0014] The nutritional enhancers described above include biomass particles, chito

oligosaccharides, polyaspartic acid, tea saponin, indole acetic acid, humus, fermented

faeces and oyster shell powder.

[0015] In particular, the ratio by weight is 3-8: 0.5: 0.5: 1-5: 0.5-1 .5: 10-15: 1-3

1-50

[0016] In particular, the coastal clay is from the Yellow River Delta and has a mesh of

100-300 mesh.

[0017] In particular, the mesh number of attapulgite clay is : 200 mesh.

[0018] Wherein the biomass particles comprise reed powder, suaeda salsa powder, corn

cob powder.

[0019] In particular, the biomass particles are prepared from reed powder, suaeda salsa

powder, corn cob powder in a weight ratio of 2:1: 2.

[0020] In particular, the biomass particles comprise reed powder, suaeda salsa powder,

corn cob powder, with the mesh number of 100-300.

[0021] In the fluid seed formulation provided by the present invention, the water

retaining material has the functions of absorbing and storing water, relieving water and

salt stress in the process of seed germination, and promoting seed germination and

seedling growth.

[0022] The coastal clay is used to increase the density of fluid seeds, so as to make the

seeds better integrate with the tidal flat soil, and effectively reduce the damage and

interference of tidal events to the soil seed bank. The organic matter itself can supply the

nutrients during seed germination and seedling growth, which provides the balanced

mineral nutrients for seedling growth and promotes the growth of root system.

[0023] Attapulgite clay, also known as palygorskite, is a water-containing Mg-Al-rich

silicate clay mineral with a chain-like structure, with a special fibrous structure, unusual

colloids and adsorption properties. In addition, that invention has the advantage of good

environmental protection and free natural pollution, and can effectively inhibit the

growth of bacteria and microorganism in the germination of seeds and seedling stage. It

has good water absorption and expansibility, and can provide a large amount of air for

seed germination while maintaining water content.

[0024] Biomass particles are organic materials that can supply nutrients during seed

germination and seedling growth which is an effective approach to the reuse of discarded

agriculture organic compounds.

[0025] Chito-oligosaccharides are low molecular weight products with good water

solubility, great function and high biological activity. It has many unique functions, such

as being completely soluble in water, easy to be absorbed and utilized by organisms. Chito

oligosaccharide can change the soil flora and promote the growth of beneficial

microorganisms, and it can also induce the disease resistance of plants, and produce

immunity and kill many kinds of fungi, bacteria and viruses.

[0026] Polyaspartic acid is a water-soluble biomimetic polymer, which can effectively

promote plant seedlings' absorption of nutritional elements, promote their absorption of

trace elements such as iron, zinc and manganese, and promote their root growth, enhance

stress resistance.

[0027] A class of glycoside compounds, tea saponin, is a natural surfactant with good

properties. It is an excellent additive of water agent or soluble powder, which can improve the physical properties of the formula of the fluid seed and its adhesion on the surface of the seed or seedling, and plays a synergistic effect on the fluid seeds.

[0028] Indole acetic acid, pure product is colorless leaf-like crystal or crystalline powder.

It can be used as a plant growth stimulant to promote the rooting of seeds and improve

the germination rate and survival rate of seeds.

[0029] Humus is a kind of brown-black or brown, amorphous, acidic, hydrophilic and

polydisperse organic matter. Humus is a complex and stable macromolecular organic

compound formed by soil organic matter under the action of microorganism. Humus is

the main component of soil organic matter. In general, it accounts for 50-70% of the total

organic matter, and the main components of humus are carbon, hydrogen, oxygen,

nitrogen, sulfur and phosphorus, etc. Humus is not only the main source of soil nutrients,

but also has an important influence on the physical, chemical and biological properties of

soil, and is one of the indicators of soil fertility. It plays an important role in the

maintenance of soil nutrients, the formation of soil structure, and the promotion of

mineral decomposition and nutrient release.

[0030] Fermented feces contain elements such as nitrogen, phosphorus and potassium

needed for plant growth, which can not only provide a large amount of nutrition for seed

germination, but also increase and renew the organic matter in the soil around seeds, and

can promote microbial reproduction, improve the physical and chemical properties and

biological activities of the soil so as to provide an excellent environment for the growth

of seedling plants in the future.

[0031] Oyster shell powder not only contains a large amount of calcium carbonate, but

also contains essential trace elements in plants, such as copper, magnesium, potassium, molybdenum, phosphorus, manganese, iron and zinc, providing balanced mineral nutrients for seedling growth and promoting the growth of the plant.

[0032] Alternatively, the weight ratio of water retaining agent, auxiliary binder and

nutrition promoter is 2-6: 20-40: 1-5.

[0033] Alternatively, the nutritional promoter is based on parts by weight, biomass

particles 5-10, chitooligosaccharide 0.5,polyaspartic acid 0.5,teasaponin5-7,indole

acetic acid 0.5-1.5, humus 15-20, fermented feces 3-5 and oyster shell powder 1-3.

[0034] Alternatively, the fermented manure is obtained by fermentation of one or more

of cow manure, pig manure, chicken manure and sheep manure.

[0035] A process for making fluid seeds for salt marsh vegetation restoration in coastal

wetlands comprises the following steps:

[0036] (1) The seashore clay, attapulgite clay and hydroxyethyl cellulose after screening

are mixed evenly in a mixer at a ratio of 30-40: 40-60:2-5 to form an auxiliary binder;

[0037] (2) After screening, biomass particles, chito-oligosaccharides, polyaspartic acid,

tea saponin, indole acetic acid and humus, the ferment excrement and oyster shell powder

are mix evenly in a stirrer according to the proportion of 3-8: 0.5: 0.5-1: 5: 0. 5-1, 5: 10

: 1-1-5: 1 to obtain a nutrition accelerator;

[0038] (3) A fluid seed formulation is prepared by mixing a water retaining agent, an

auxiliary binder, and a nutritional promoter in a ratio of 1-5: 10-20: 1 to 5 by weight in a

blender.

[0039] (4) The fluid seed formulation, seeds and water shall be uniformly stirred at a

weight ratio of 4: 1: 40-60 until a suspension is formed and shall be spread through a

specific spraying device.

[0040] Alternatively, the seed may be selected from any of the types of vegetation in the

coastal wetland having a diameter of 5 mm or more.

BRIEF DESCRIPTION OF THE FIGURES

[0041] FIG. 1 is a line graph of the daily germination rate of fluid seeds of Suaeda salsa

seeds manufactured in Example 1 under each salinity stress (0 g/kg, 5 g/kg, 10 g/kg and

g/kg, 20 g/kg)

[0042] FIG. 2 is a bar graph comparing the cumulative germination rates of the fluid seeds

of Suaeda salsa seeds manufactured in Example 1 with those of common seeds under

each salinity stress (0 g/kg, 5 g/kg, 10 g/kg and 15 g/kg, 20 g/kg).

DESCRIPTION OF THE INVENTION

[0043] The present invention will be further described in detail below in conjunction with

examples. In the process of describing the present invention, for related known

technologies or structures, if the description is deemed to unnecessarily obscure the gist

of the present invention, the detailed description will be omitted. In addition, the

following words are words that are defined in consideration of the functions of the present

invention, which may be different according to the intentions or conventions ofusers and

operators, and should be defined according to the content of the entire specification of

the present invention. If specific conditions are not indicated in the examples, the routine

conditions or the conditions recommended by the manufacturer are used. The reagents or instruments used without the manufacturer's indication are all conventional products that can be purchased commercially.

[0044] The present invention provides a fluid seed formulation used for vegetation

restoration of coastal wetland tide beach salt marsh, which includes a water retaining

agent, an auxiliary adhesive, and a nutrition promoter. Among them, in terms of parts by

weight, the ratio of the water-retaining agent, auxiliary adhesive and nutrition promoter is

1-5:10-20:1-5. More preferably, the ratio of the water-retaining agent, the auxiliary

binder and the nutrition enhancer is 2-6:20-40:1-5; the water-retaining agent, the water

retaining material is hydroxyethyl cellulose; The components of the auxiliary binder

include: 30-40 parts of coastal clay, 40-60 parts of attapulgite clay, and 2-5 parts of

hydroxyethyl cellulose; the components of the nutrition promoter include: biomass

particles 3 -8 parts, chito-oligosaccharide 0.5 parts, polyaspartic acid 0.5 parts, tea

saponin 1-5 parts, indole acetic acid 0.5-1.5 parts, humus 10-15 parts, fermented feces 1

3 servings and 1-5 servings of oyster shell powder. More preferably, the components of

the nutrition enhancer include: 5-10 parts of biomass particles, 0.5 parts of

chitooligosaccharides, 0.5 parts of polyaspartic acid, 5-7 parts of tea saponin, and indole

0.5-1.5 parts of inole acetic acid, 15-20 parts of humus, 3-5 parts of fermented manure

and 1-3 parts of oyster shell powder; the coastal clay is taken from the Yellow River

Delta, and its grain number is 100-300 mesh The number of particles of the attapulgite

clay lgite clay 20 parts of humus, 3-5 parts of fermented manure and 1-3 parts of oyder, 1

part of Suaeda powder, 2 parts of corn cob powder; The mesh number is 100-300; the fermented manure is obtained by fermentation of one or more of cow manure, pig manure, chicken manure and sheep manure.

[0045] The present invention also provides a preparation method of fluid seeds for

vegetation restoration of tidal flat salt marshes in coastal wetlands, including the

following steps:

[0046] Step 101: The sieved littoral clay, attapulgite clay and hydroxyethyl cellulose are

uniformly mixed in a mixer to form an auxiliary binder.

[0047] In addition, in order to make the auxiliary binder have good bonding and weight

gain effects, preferably, the mass ratio of coastal clay to attapulgite clay is 30-40: 40

: 2-5.

[0048] Step 102: The sieved biomass particles, chitooligosaccharides, polyaspartic acid,

tea saponin, indole acetic acid, humus, fermented feces and oyster shell powder are

uniformly mixed in a mixer to prepare a nutrition promoter.

[0049] Preferably, in order to achieve a better nutritional promotion effect, the mass ratio

of biomass particles, chitooligosaccharides, polyaspartic acid, tea saponin, indole acetic

acid, humus, fermented feces and oyster shell powder is 3-8: 0.5:0.5:1-5:0.5-1 .5:10

:1-3:1-5.

[0050] Step 103: Mix the water-retaining agent, auxiliary binder, and nutrient enhancer

in a mixer to prepare a fluid seed formula.

[0051] Preferably, in the actual operation process, in order to optimize the effect of the

fluid seed, the mass ratio of the water-retaining agent, auxiliary adhesive and nutrition

promoter is 1-5:10-20:1-5.

[0052] Step 104: The fluid seed formula, seeds, and water are stirred until a suspension

is formed, and then the suspension is sown through a specific sprinkling device.

[0053] Preferably, in order to optimize the sowing effect of the fluid seeds, the

suspension state of the seeds is the best, and the mass ratio of the fluid seed formulation,

the seeds and the water is 4:1:40-60.

[0054] Preferably, the seed may be selected from any vegetation type with a seed

diameter of 5 mm or more in coastal wetlands.

[0055] In the fluid seed formulation provided by the present invention, the water

retaining material has the functions of absorbing and storing water, alleviating water and

salt stress during seed germination, and promoting seed germination and seedling growth.

[0056] The coastal clay is used to increase the density of fluid seeds, better integrate with

the tidal flat soil, effectively reduce the damage and interference of tidal events to the soil

seed bank, and play a role in wave prevention; the organic matter contained in it can

provide seed germination and The nutrients during the seedling period provide balanced

mineral nutrient elements for the growth of the seedling period and promote the

development and growth of the root system during the seedling period.

[0057] Attapulgite clay, also known as palygorskite, is a kind of water-rich magnesium

aluminosilicate clay mineral with chain layered structure, with special fiber structure,

unusual colloids and adsorption properties. In addition, that invention has the advantage

of good environmental protection and natural pollution-free, and can effectively inhibit

the germination of seeds and the growth of bacteria and microorganism in seedling stage.

It has good water absorption and expansibility and can provide a large amount of air for

seed germination while maintaining water content.

[0058] Biomass particles are organic materials that can supply nutrients during seed

germination and seedling stage, and are an effective way to reuse agricultural wastes.

[0059] Chitooligosaccharides are low molecular weight products with good water

solubility, great function and high biological activity. It has many unique functions, such

as being fully soluble in water, easy to be absorbed and utilized by organisms. Chito

oligosaccharide can change the soil flora and promote the growth of beneficial

microorganisms, and it can also induce the disease resistance of plants, and produce

immunity and killing effects on a variety of fungi, bacteria and viruses.

[0060] Polyaspartic acid is a water-soluble biomimetic polymer that can effectively

promote the absorption of nutritional elements by plant seedlings, promote their

absorption of trace elements such as iron, zinc and manganese, and promote their root

growth and enhance stress resistance.

[0061] Tea saponin is a kind of glycoside compound, which is a natural surfactant with

good performance. It is an excellent additive of water agent or soluble powder, which can

improve the physical properties of the formula of the fluid seed, increase its adhesion on

the surface of the seed or seedling, and play a synergistic effect on fluid seeds.

[0062] Indole acetic acid, pure product is colorless leaf crystal or crystalline powder. It

can be used as a plant growth stimulant to promote the rooting of seeds and increase the

germination rate and survival rate of seeds.

[0063] Humus is a kind of brown-black or brown, amorphous, acidic, hydrophilic and

polydisperse organic matter. Humus is a complex and stable macromolecular organic

compound formed by soil organic matter under the action of microorganism. Humus is

the main component of soil organic matter. In general, it accounts for 50-70% of the total

organic matter, and the main components of humus are carbon, hydrogen, oxygen,

nitrogen, sulfur and phosphorus, etc. Humus is not only the main source of soil nutrients,

but also has an important influence on the physical, chemical and biological properties of

soil, and is one of the indicators of soil fertility. It plays an important role in the

maintenance of soil nutrients, the formation of soil structure, and the promotion of

mineral decomposition and nutrient release.

[0064] Fermented feces contain elements such as nitrogen, phosphorus and potassium

needed for plant growth, which can not only provide a large amount of nutrients for seed

germination, but also increase and renew the organic matter in the soil around seeds, and

promote microbial reproduction. Improve the physical and chemical properties and

biological activities of the soil, and provide an excellent environment for the growth of

seedling plants in the future.

[0065] Oyster shell powder not only contains a large amount of calcium carbonate, but

also contains essential trace elements in plants, such as copper, magnesium, potassium,

molybdenum, phosphorus, manganese, iron and zinc, providing balanced mineral

nutrients for seedling growth. Promote the growth of the plant.

[0066] The action of fluid seeds mixed in the above-mentioned weight ratio on the

auxiliary binder after being sprayed onto the tidal flat. In combination with that soil

slowly, the method effectively reduce the damage and disturbance of the soil seed bank cause by the tidal event, and the water-retaining agent in the soil absorbs water and swells every time the tide come, A series of "mini-reservoirs" are formed in the operation area, which provide water for seed germination and seedling growth, effectively cope with the inhibition of spring drought on seed germination, and at the same time reduce the salinity of soil around the area. The seed germination was promoted and the survival rate of seeds and seedlings was increased significantly. The fluid seed formula has the advantages of pertinence, universality, wave resistance, drought resistance, salt and alkali resistance and nutrition. In addition, that raw material component are mostly pure natural components, are environment-friendly and pollution-free, and meet the requirement of ecological restoration of the coastal wetland.

[0067] In addition, the fluid seed has a simple manufacturing method, low cost, simple

actual operation process, and is suitable for large-area tidal flat operations.

[0068] To make the objectives, technical solutions, and advantages of the embodiments

of the present invention clearer, the following will clearly and completely describe the

technical solutions in the embodiments of the present invention with reference to the

accompanying drawings in the embodiments of the present invention. Obviously, the

described embodiments are Some embodiments of the present invention, but not all

embodiments. Based on the embodiments of the present invention, all other embodiments

obtained by those of ordinary skill in the art without creative work shall fall within the

protection scope of the present invention.

[0069] Example 1: Preparation of Suaeda salsa fluid seeds

[0070] 1. Pretreatment of the seeds of Suaeda salsa:

[0071] Seed shall be collected and placed in a ventilated place, and after being completely

dried, natural air selection or mechanical air selection shall be adopted to remove

impurities, dust, dead branches and unfull seeds, so as to obtain pre-treated seeds of Suaeda

salsa.

[0072] 2. Preparation of fluid seed formulations:

[0073] 1) Preparation of water retention agent:

[0074] 1-A) The water-retaining material shall be crushed so that the particle size of the

water-retention material is more than 200 mesh, and the water retaining material is

hydroxyethyl cellulose.

[0075] 2) Preparation of auxiliary adhesives:

[0076] 2-A) Crushing coastal clay, attapulgite clay and hydroxyethyl cellulose respectively

to prepare coastal clay, palygorskite clay, hydroxyethylcellulose respectively with a

particle size more 200 mesh.

[0077] 2-B) Mixing the coastal clay, attapulgite clay and hydroxyethyl cellulose in a mixer

to obtain an auxiliary binder for future use. The weight ratio of seashore clay, attapulgite

clay and hydroxyethyl cellulose is 6: 10: 1.

[0078] 3) Preparation of nutritional promoters:

[0079] 3-A) Crushing reed, suaeda salsa, corn cobs, chito-oligosaccharides, polyaspartic

acid, tea saponin, indole acetic acid, humus, fermented feces and oyster shell powder

respectively. Herba Phragmites australis, Suaeda salsa, corn cob, oligochitosan,

polyaspartic acid, tea saponin, indole acetic acid, humus, ferment excrement and oyster

shell powder with particle size more than 200 mesh were prepared.

[0080] 3-B) Mix the reed powder, suaeda salsa powder and corn cob powder evenly to

prepare biomass granules waiting for use, wherein the weight ratio of reed powder to

suaeda heteroptera powder and corns powder is 2: 1: 2.

[0081] 3-C) Mix biomass particles, chito-oligosaccharides, polyaspartic acid, tea saponin,

indole acetic acid, humus, fermented excrement and oyster shell powder evenly to prepare

a middle coating agent for use. The weight ratio of biomass particles,

chitooligosaccharides, polyaspartic acid, teasaponin, indole acetic acid, humus, fermented

feces and oyster shell powder is 5: 0.5: 0. 5: 2: 0: 10: 2.

[0082] 3. Preparing fluid seeds

[0083] 1) 18g of water-retaining agent, 364g of formulated auxiliary binder and 18g of

formulated nutrition-promoting agent were successively put into a stirrer, and stirred for 5

minutes in an adequate amount to prepare a fluid seed formulation. Wherein the mass of

the added water retaining agent is about 5% of the weight of the auxiliary binder, that is 5

g of the water retention agent is required for every 100 g of auxiliary binder added; and the

masses of the nutritional promoters added are about 5 percent of the mass thereof, that is,

for every 100 g of the auxiliary binder added, 5 g of a nutritional promoter is added.

[0084] 2) The fluid seed formulation and Suaeda salsa seeds were added in turn to a

blender, stirred thoroughly for 5 minutes, and then added a certain mass of water, stirred

throughly for 10 minutes until a stable suspension was formed and a fluid seed for Suaeda

salsa was prepared. Wherein the mass of the fluid seed formulation added is about 400%

of the seed mass of Suaeda salsa, that is, 400 g of the fluid seed formulation is required for

every 100 g of suaeda salsa seeds added; the volume of water added is about 5000% of the seed mass of supress salsa, that is 5000 ml of water is required per 100 g the seed mass of

Suaeda salsa.

[0085] Example 2: Preparation of Suaeda salsa fluid seeds

[0086] 1. Pretreatment of the seeds of Suaeda salsa:

[0087] Seed shall be collected and placed in a ventilated place, and after being completely

dried, natural air selection or mechanical air selection shall be adopted to remove

impurities, dust, dead branches and unfull seeds, so as to obtain pre-treated seeds of Suaeda

salsa.

[0088] 2. Preparation of fluid seed formulations:

[0089] 1) Preparation of water retention agent:

[0090] 1-A) The water-retaining material shall be crushed so that the particle size of the

water-retention material is more than 200 mesh, and the water retaining material is

hydroxyethyl cellulose.

[0091] 2) Preparation of auxiliary adhesives:

[0092] 2-A) Crushing coastal clay, attapulgite clay and hydroxyethyl cellulose respectively

to prepare coastal clay palygorskite clay, hydroxyethylcellulose with a particle size more

than 200 mesh.

[0093] 2-B) Mixing evenly coastal clay, attapulgite clay and hydroxyethyl cellulose in a

mixer to obtain an auxiliary binder for use. The weight ratio of seashore clay, attapulgite

clay and hydroxyethyl cellulose is 15: 20: 1.

[0094] 3) Preparation of nutritional promoters:

[0095] 3-A) Crushing reed, Suaeda salsa, corn cobs, chito-oligosaccharides, polyaspartic

acid, tea saponin, indole acetic acid, humus, fermented feces and oyster shell powder respectively. Herba Phragmites australis, Suaeda salsa, corn cob, oligochitosan, polyaspartic acid, tea saponin, indole acetic acid, humus, ferment excrement and oyster shell powder with particle size 200 mesh were prepared.

[0096] 3-B) Mix the reed powder, suaeda salsa powder and corn cob powder evenly to

prepare biomass granules, wherein the weight ratio of reed powder to suaeda heteroptera

powder and corns powder is 2: 1: 2.

[0097] 3-C) Mix biomass particles, chito-oligosaccharides, polyaspartic acid, tea saponin,

indole acetic acid, humus, fermented excrement and oyster shell powder evenly to prepare

a middle coating agent for use. The weight ratio of biomass particle, chitooligosaccharide,

polyaspartic acid, tea saponin, indole acetic acid, humus, fermented excrement and oyster

shell powder is 3: 0.5: 0.5: 1: 0.5: 10: 1: 1.

[0098] 3. Preparation of fluid seeds

[0099] 1) 18g of water-retaining agent, 364g of formulated auxiliary binder and 18g of

formulated nutrition-promoting agent were successively put into a stirrer, and stirred for 5

minutes in an adequate amount to prepare a fluid seed formulation. Wherein the mass of

the added water retaining agent is about 5% of the weight of the auxiliary binder, that is, 5

g of the water retention agent is required for every 100 g of auxiliary binder added; and the

masses of the nutritional promoters added are about 5 percent of the mass thereof, that is,

for every 100 g of the auxiliary binder added, 5 g of a nutritional promoter is added.

[0100] 2) Add the fluid seed formula and Suaeda salsa seeds into the stirrer in turn, stir

them for 5 minutes, add water of a certain quality, and stir them fully for 10 minutes to

form a stable suspension to obtain the fluid seeds of suaeda salsa. Wherein the mass of the

fluid seed formulation added is about 400% of the seed mass of Suaeda salsa, that is, 400 g of the fluid seed formulation is required for every 100 g of suaeda salsa seeds added; the volume of water added is about 600% of the seed mass of supress salsa, that is 6000 ml of water is required per 100 g fluid seeds of suaeda salsa.

[0101] Example 3: Preparation of Suaeda salsa fluid seeds

[0102] 1. Pretreatment of the seeds of Suaeda salsa:

[0103] Seed shall be collected and placed in a ventilated place, and after being completely

dried, natural air selection or mechanical air selection shall be adopted to remove

impurities, dust, dead branches and unfull seeds, so as to obtain pre-treated seeds of Suaeda

salsa.

[0104] 2. Prepare the fluid seed formulation:

[0105] 1) Preparation of a water retaining agent:

[0106]1-A) Crushing the water-retaining material such that the particle size of the water

retention material is more then 200 mesh, and the water retaining material is hydroxyethyl

cellulose.

[0107]2) Preparation of auxiliary adhesive:

[0108] 2-A) Crushing coastal clay, attapulgite clay and hydroxyethyl cellulose respectively

to prepare coastal clay palygorskite clay, hydroxyethylcellulose with a particle size more

than 200 mesh.

[0109] 2-B) Mixing coastal clay, attapulgite clay and hydroxyethyl cellulose evenly in a

mixer to obtain an auxiliary binder for use. The weight ratio of seashore clay, attapulgite

clay and hydroxyethyl cellulose is 8: 12: 1.

[0110] 3) Preparation of nutritional promoters:

[0111] 3-A) Crushed reed, Suaede salsa, corn cobs, chito-oligosaccharides, polyaspartic

acid, tea saponin, indole acetic acid, humus, fermented feces and oyster shell powder

respectively. Herba Phragmites australis, Suaede salsa, corn cob, oligochitosan,

polyaspartic acid, tea saponin, indole acetic acid, humus, ferment excrement and oyster

shell powder with particle size : 200 mesh were prepared.

[0112] 3-B) Mix the reed powder, Suaede salsa powder and corn cob powder evenly to

prepare biomass granules, wherein the weight ratio of reed powder to Suaede heteroptera

powder is 2: 1: 2.

[0113] 3-C) Mix biomass particles, chito-oligosaccharides, polyaspartic acid, tea saponin,

indole acetic acid, humus, fermented excrement and oyster shell powder evenly to

prepare a middle coating agent for use. The weight ratio of biomass particles,

chitooligosaccharides, polyaspartic acid, teasaponin, indole acetic acid, humus,

fermented excrement and oyster shell powder is 8: 0.5: 0.5: 1.5: 15: 3: 5.

[0114] 3. Preparation of fluid seeds

[0115] 1) 40g of water retaining agent, 320g of formulated auxiliary binder and 40g the

formulated nutrition promoter was successively put into a stirrer, and stirred thoroughly

for 5 minutes to prepare a fluid seed formulation. Wherein the mass of the water retaining

agent added is about 12.5% by weight of the auxiliary binder, that is, 12.5 g of a water

retaining agent is required to add per 100 g of the auxiliary binder; the mass of the

nutritional promoter added is about 12.5% of the mass for the auxiliary adhesive, that is,

12.5 g of a nutritional promoter is required to add for every 100 g of auxiliary binder

added.

[0116] 2) The fluid seed formula and the seeds of Suaede salsa were sequentially added

to a stirrer, stirred for 5 minutes, then added with water of a certain quality, and stirred

for 10 minutes until a stable suspension was formed and the fluid seeds of Suaeda salsa is

obtained. Wherein the mass of the fluid seed formulation added is about 400% of the

seed mass of Suaede salsa, that is, 400 g of the fluid seed formulation is required for

every 100 g of Suaede salsa seeds added; the volume of water added is about 4000% of

the seed mass of supress salsa, that is 4000 ml of water is required per 100 g the fluid

seeds of Suaeda salsa.

[0117] Example: Laboratory simulated germination test of fluid seeds of Suaede salsa

[0118] Take seeds of Suaede salsa with full grains, disinfect them with 0.1% potassium

permanganate solution for 8 minutes, rinse them with deionized water for 3 times, and

soak them in normal temperature to promote germination for 6 hours. There were two

treatments in the experiment. The control group was untreated naked seeds, and the

experimental group was saline Suaede salsa seeds added with fluid seed formula, and

each treatment was repeated in 5 groups. Placing 100 (about 0.33 g) seeds of Suaede

salsa in each culture dish, wherein the culture dishes of the experimental group were

added with 1.32 g of the fluid seed formulation. Add a proper amount of saline water

(divided into five salinity gradients of 0 g/kg, 5 g/kg, 10 g/kg and 15 g/kg as well as 20

g/kg), and the depth is inclined at 45 0 and the water does not overflow, so as to provide

the necessary conditions for its germination. In each culture dish, 100 seed are placed and

alternately culture under light for 12 hours / dark for 12 hours. during that culture

process, the cover of the culture dishes is covered to reduce the loss of water. The amount of germination was recorded at 6 o'clock in the afternoon, and the germination was regarded as the emergence of 2 mm radicle, the experiment was terminated after 10 days of continuous culture until no seed germination was found for 3 days.

[0119] In this test, the Suaede salsa fluid seeds of Example 1 of the present invention

were used.

[0120] The results are shown in Figure 1 and Figure 2. the test results show that:

[0121] 1. There was no significant difference in germination rate of fluid seeds under

moderate and high salinity stress (10, 15, 20 g/kg (P<0 .05), indicating that the fluid

seeds have good salt tolerance to medium and high salinity.

[0122] 2. The germination rate of fluid seeds was significantly higher than that of bare

seeds under all salinity stresses (Og/kg, 5g/kg, lOg/kg, 15g/kg, 20 g/kg), indicating that

the seed products could promote the germination of Suaede salsa seeds.

[0123] Although the invention has been described with reference to specific examples, it

will be appreciated by those skilled in the art that the invention may be embodied in

many other forms, in keeping with the broad principles and the spirit of the invention

described herein.

[0124] The present invention and the described preferred embodiments specifically

include at least one feature that is industrial applicable.

Claims (11)

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:

1. A fluid seed formula used for vegetation restoration of tidal flat salt marshes of a coastal wetland, comprising a water retaining agent, an auxiliary binder and a

nutrition promoter.

2. The fluid seed formulation according to claim 1, wherein the weight ratio of the water-retaining agent, the auxiliary binder and the nutrition promoter is 1: 5: 20 : 1-5.

3. The fluid seed formulation according to claim 1, wherein the water retaining agent is hydroxyethyl cellulose, the auxiliary binder is composed of coastal clay, attapulgite clay and hydroxyethylcellulose, in an amount of 30-40: 40-60: 2-5 by weight, and that nutrition promoter comprises biomas particles, chito oligosaccharides, polyaspartic acid, tea saponin, indole acetic acid, humus, fermented feces and oyster shell powder, in an amount of 3-8 : 0.5 : 0.5 : 1-5 : 0.5-1 .5 : 10-15

: 1-3 : 1-5 by weight.

4. The fluid seed formulation according to claim 3, wherein the number of coastal clays is 100-300.

5. The fluid seed formulation according to claim 3, wherein the number of meshes of the attapulgite clay is 200 or more.

6. The fluid seed formula according to claim 3, wherein the biomass particles comprise reed powder, suaeda salsa powder and corn cob powder.

7. The fluid seed formula according to claim 3 wherein the biomass particles are composed of reed powder, suaeda salsa powder and corn cob powder mixed in a weight ratio of 2: 1: 2.

8. The fluid seed formula according to claim 3, wherein the biomass particles comprise reed powder, suaeda salsa powder and corn cob powder, the mesh number of which is 100-300 mesh.

9. The fluid seed formulation according to claim 3, wherein the fermented manure is obtained by fermenting one or more of cow manure, pig manure, chicken manure and sheep manure.

10. A manufacturing process of a wave-resistant, drought-resistant and salt

tolerant fluid seed used for the degradation of tidal flat vegetation of a coastal wetland, comprising the steps of:

(1) Mixing the sieved coastal clay, attapulgite clay and hydroxyethyl cellulose in a ratio of 30-40: 40-60: 2-5 by weight in a stirrer to make an auxiliary binder;

(2) After screening, biomass particles, chitooligosaccharides, polyaspartic acid, tea saponin, indoleacetic acid, humus, fermented faeces and oyster shell powder were mixed evenly in a blender in the proportion of 3-8:0.5:0.5:1-5:0.5-1.5:10-15:1-3:1-5 to obtain nutritional promoters;

(3) Mixing the water retaining agent, the auxiliary binder and the nutrition

promoter in a ratio of 1: 5: 10-20: 1-5 by weight in a stirrer to prepare a fluid seed formulation;

(4) Stirring the fluid see formula, the seed and the water uniformly at a weight ratio of 4: 1: 40-60 until a suspension is formed, and the suspension is scattered through a specific spray device.

11. A process for producing a fluid seed for vegetation restoration of a tidal flat salt marsh of a coastal wetland according to claim 7, the seed can be selected from any vegetation type with a seed diameter of more than 5mm in the coastal wetland.

Germination rate (%) -1/2-

Fig. 1 Time

Cumulative germination rate

Salinity (ppt) -2/2-

Fig. 2 Fluid seed Naked seed