CN113677696A - 一种培育抗灰斑病植物的方法 - Google Patents
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- CN113677696A CN113677696A CN202080018758.1A CN202080018758A CN113677696A CN 113677696 A CN113677696 A CN 113677696A CN 202080018758 A CN202080018758 A CN 202080018758A CN 113677696 A CN113677696 A CN 113677696A
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
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Abstract
本发明公开了一种培育抗灰斑病植物的方法。本发明提供的蛋白质，获自玉米，命名为ZmPK蛋白，为序列表中序列2、序列4、序列7或序列9所示的蛋白质。编码ZmPK蛋白的核酸分子也属于本发明的保护范围。本发明还保护一种制备转基因植物的方法，包括如下步骤：在出发植物中导入所述核酸分子，得到灰斑病抗病性降低的转基因植物。本发明还保护一种制备转基因植物的方法，包括如下步骤：敲除或抑制出发植物中所述核酸分子的表达，得到灰斑病抗病性增高的转基因植物。本发明对于玉米的抗灰斑病育种具有重大的应用价值。
Description
PCT国内申请，说明书已公开。
Claims (22)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910160206.3A CN109705202B (zh) | 2019-03-04 | 2019-03-04 | 一种培育抗灰斑病植物的方法 |
| CN2019101602063 | 2019-03-04 | ||
| PCT/CN2020/076319 WO2020177560A1 (zh) | 2019-03-04 | 2020-02-24 | 一种培育抗灰斑病植物的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113677696A true CN113677696A (zh) | 2021-11-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910160206.3A Active CN109705202B (zh) | 2019-03-04 | 2019-03-04 | 一种培育抗灰斑病植物的方法 |
| CN202080018758.1A Pending CN113677696A (zh) | 2019-03-04 | 2020-02-24 | 一种培育抗灰斑病植物的方法 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201910160206.3A Active CN109705202B (zh) | 2019-03-04 | 2019-03-04 | 一种培育抗灰斑病植物的方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20220170041A1 (zh) |
| EP (1) | EP3936519A4 (zh) |
| CN (2) | CN109705202B (zh) |
| BR (1) | BR112021017043A2 (zh) |
| MX (1) | MX2021010663A (zh) |
| WO (1) | WO2020177560A1 (zh) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109705202B (zh) * | 2019-03-04 | 2022-05-10 | 中国农业大学 | 一种培育抗灰斑病植物的方法 |
| CN113444160A (zh) * | 2021-04-25 | 2021-09-28 | 东北农业大学 | ZmNuC基因及其编码蛋白在抗玉米粗缩病中的应用 |
| CN114262369B (zh) * | 2021-12-15 | 2023-05-16 | 中国农业大学 | ZmDi19基因及其靶标基因ZmPR10在培育抗灰斑病植物中的应用 |
| CN114410651B (zh) * | 2022-01-06 | 2024-02-20 | 中国农业大学 | 玉米灰斑病抗性相关蛋白及其编码基因与应用 |
| CN117209575A (zh) * | 2023-05-29 | 2023-12-12 | 中国农业大学 | 蛋白质及其编码基因在调控玉米大斑病和小斑病中的应用 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525780A (zh) * | 2013-10-08 | 2014-01-22 | 北京大学 | 水稻钙依赖性蛋白激酶基因及其应用 |
| CN105821016A (zh) * | 2016-04-21 | 2016-08-03 | 南京农业大学 | 一种大豆cdpk类激酶及其编码基因与应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8367899B2 (en) * | 2007-12-31 | 2013-02-05 | E I Du Pont De Neumours And Company | Gray leaf spot tolerant maize and methods of production |
| CN105218651B (zh) * | 2015-09-01 | 2019-05-28 | 中国农业大学 | 玉米抗禾谷镰刀菌茎腐病基因ZmAuxRP1的克隆及功能分析 |
| CN105859860A (zh) * | 2016-05-18 | 2016-08-17 | 中国科学院遗传与发育生物学研究所 | 抗病相关蛋白在调控植物抗病性中的应用 |
| EA201990256A1 (ru) * | 2016-07-11 | 2019-07-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Способы получения маиса, устойчивого к серой пятнистости листьев |
| CN106701972B (zh) * | 2017-01-23 | 2019-07-16 | 华中农业大学 | 玉米抗灰斑病主效qtl的连锁分子标记和应用 |
| CN109705202B (zh) * | 2019-03-04 | 2022-05-10 | 中国农业大学 | 一种培育抗灰斑病植物的方法 |
-
2019
- 2019-03-04 CN CN201910160206.3A patent/CN109705202B/zh active Active
-
2020
- 2020-02-24 CN CN202080018758.1A patent/CN113677696A/zh active Pending
- 2020-02-24 MX MX2021010663A patent/MX2021010663A/es unknown
- 2020-02-24 BR BR112021017043A patent/BR112021017043A2/pt unknown
- 2020-02-24 US US17/434,206 patent/US20220170041A1/en active Pending
- 2020-02-24 EP EP20767245.2A patent/EP3936519A4/en active Pending
- 2020-02-24 WO PCT/CN2020/076319 patent/WO2020177560A1/zh unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525780A (zh) * | 2013-10-08 | 2014-01-22 | 北京大学 | 水稻钙依赖性蛋白激酶基因及其应用 |
| CN105821016A (zh) * | 2016-04-21 | 2016-08-03 | 南京农业大学 | 一种大豆cdpk类激酶及其编码基因与应用 |
Non-Patent Citations (2)
| Title |
|---|
| "登记号NM_001359579" * |
| 徐凌: "玉米抗灰斑病主效QTL精细定位、基因预测及转录组分析" * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109705202A (zh) | 2019-05-03 |
| CN109705202B (zh) | 2022-05-10 |
| EP3936519A1 (en) | 2022-01-12 |
| EP3936519A4 (en) | 2022-12-14 |
| BR112021017043A2 (pt) | 2021-11-16 |
| MX2021010663A (es) | 2021-09-28 |
| US20220170041A1 (en) | 2022-06-02 |
| WO2020177560A1 (zh) | 2020-09-10 |
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