CN111700927B - Medicinal and edible composition with blood sugar reducing effect and preparation method and application thereof - Google Patents
Medicinal and edible composition with blood sugar reducing effect and preparation method and application thereof Download PDFInfo
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Abstract
The invention discloses a medicinal and edible composition with the function of reducing blood sugar, a preparation method and application thereof, wherein the composition is prepared by mixing extracts of eucommia ulmoides, lithocarpus litseifolius and gynura procumbens, and the preparation method comprises the following steps: (1) drying, pulverizing and sieving Eucommiae cortex, Litsea Coreana Hayata and Gynura procumbens to obtain Eucommiae cortex, Litsea Coreana Hayata and Gynura procumbens powder; (2) respectively extracting the eucommia bark, lithocarpus litseifolius and gynura procumbens powder by using a solvent, precipitating with ethanol, extracting and crystallizing to obtain eucommia bark, lithocarpus litseifolius and gynura procumbens extracts; (3) mixing cortex Eucommiae, Lithocarpus litseifolius (wall.) Szel and Gynura procumbens (wall.) Druce extract at a ratio of 1:1:1-1:1:3, and shaking to obtain combined extract. The medicinal and edible composition produced by the invention can obviously reduce the blood glucose index and the postprandial blood glucose content of diabetics, has good prevention and treatment effects on complications caused by diabetes, and has obvious social and economic benefits.
Description
Technical Field
The invention belongs to the technical field of extraction and application of effective active ingredients of plants, and particularly relates to a medicinal and edible composition with a blood sugar reducing effect, and a preparation method and application thereof.
Background
Diabetes refers to a disease of glucose metabolism disorder caused by insufficient insulin secretion, insulin dysfunction, and glucose metabolism disorder, and its symptoms are polydipsia, polyphagia, polyuria, and weight loss. Diabetes is one of the biggest emergent health events in the world of the 21 st century, and the global prevalence rate is continuously rising, currently affecting 8.8% of adults. Diabetes can induce a variety of acute or chronic complications, such as cardiovascular and cerebrovascular diseases, diabetic nephropathy, and the like. The continuous disturbance of glycolipid metabolism can cause a plurality of diseases of large and small blood vessels, micro blood vessels, nerves and other systems, and cause the abnormality of tissue structure and function. Diabetes is known in the medical community as the "source of all diseases" and endangers the health of people in the world. Most of the medicines are chemically or biologically synthesized, so that the blood sugar reducing effect is obvious, but a lot of side effects are brought, and the expensive price is also forbidden. In recent years, research on extracting effective components from natural products for preventing and treating diabetes has become a hot spot. Therefore, it is a great development trend to find natural medicines and health foods with high efficiency, low toxicity and low price from the Chinese medicines with abundant resources.
The term "gynura procumbens" is known as "gynura procumbensGynura procumbens(Lour.) Merr.) "is a plant of Panax genus of Compositae family, and is also called Sambucus nigra, herba Saussureae Cordatae, Angelica keiskei, is pungent, slightly bitter and cool in taste, is a perennial herbal and edible plant, and is widely distributed in China, Malaysia, Thailand, Indonesia, Korea, Philippines and other countries. Gynura procumbens has wide pharmacological effects, in the first half of 2009, toxicology inspection, hygiene inspection and inspection of various components are completed by relevant units such as the Chinese disease prevention and control center, the national food quality supervision and inspection center, the Chinese food fermentation industry research institute and the like, and the conclusion is that: no toxicity and no teratogenic influence; the effective components have the effects of clearing and activating the channels and collaterals, diminishing inflammation and relieving cough and dissipating blood stasisDetumescence, promoting blood circulation and promoting granulation, and can be used for treating traumatic injury, bronchopneumonia, and pulmonary tuberculosis; can delay aging, activate immunocyte, improve organism immunity, enhance metabolism and improve dysmnesia; has the obvious effects of reducing blood pressure, blood fat and blood sugar, resisting oxidation and ulcer, preventing chronic nephropathy and inhibiting hepatitis B; has certain curative effect on preventing and treating cardiovascular and cerebrovascular diseases, diabetes and the like; it also has antiviral, antibacterial, and bone marrow cancer and shiga-like toxin cell inhibiting effects. On the other hand, gynura procumbens is a unique plant which can be used as both medicine and food and has extremely good nutritional value, so that the gynura procumbens is widely applied to the fields of food and medicine industry, daily chemical industry and the like and is a plant which can be used as both medicine and food and has extremely high potential and high economic value.
Lithocarpus litseifolius (wall.) Rehd.)Lithocarpus polystachyusRehd), also named lithocarpus polystachyus, hydrangea strigosa, lithocarpus polystachyus. The wild state is mostly distributed in the provinces of south of the Yangtze river, and the resources of Jiangxi, Hunan, Fujian, Guangxi, Anhui and other provinces are most concentrated. Phlorizin and trilobatin are used as main components, and the content is up to 9.0-14.60%. Modern medical research shows that Lithocarpus litseifolius has effects of clearing heat, promoting diuresis, moistening lung, relieving cough, nourishing liver and kidney, etc., can be used for preventing and treating warm dysentery, carbuncle, malignant boil, skin pruritus, etc., and also has effects of 'three resisting' and 'three reducing'. Modern researches show that Lithocarpus litseifolius has pharmacological effects of resisting oxidation, reducing blood sugar, reducing blood lipid, resisting bacteria, resisting inflammation, resisting allergy, etc.
Eucommia ulmoides (eucommia ulmoides)Eucommia ulmoidesOliver), also known as Bakelite, is a plant of the genus eucommia of the family Eucommiaceae. The eucommia ulmoides oliv extract is a unique and precious nourishing traditional Chinese medicinal material in China, has high medicinal value, and has the effects of resisting aging, resisting oxidation, reducing blood pressure, reducing blood fat, reducing blood sugar, resisting mutagenesis, resisting cancer, resisting bacteria, resisting viruses and the like according to modern pharmacological research. The eucommia ulmoides has rich active ingredients, mainly contains flavonoids, phenylpropanoids, iridoids, polysaccharides, polyphenols, phenylpropanoids, lignans and the like, has outstanding pharmacological action of the eucommia ulmoides, and has various health-care effects. Eucommia bark is treasure throughout the body, and its seeds, flowers, bark and leaves can be eaten. Through toxicological identification, eucommia ulmoidesThe seed oil and the eucommia male flowers are safe and nontoxic, the seed oil and the eucommia male flowers are approved as new food raw materials by the national Wei Jie Commission, and the eucommia leaves are also approved by the national Wei Jian Commission in 2019 to list a human medicine and food homology test list, so that the eucommia leaves have great prospects in the aspect of health food development.
Disclosure of Invention
The invention aims to provide a medicinal and edible composition with a hypoglycemic effect and a preparation method and application thereof, wherein different extraction and purification modes are utilized to respectively enrich effective components in eucommia ulmoides, lithocarpus litseifolius and gynura procumbens, the obtained powders are combined in an optimized combination mode, and the hypoglycemic activity test is carried out on the composition.
In order to achieve the purpose, the invention adopts the following technical scheme:
a medicine-food homologous composition with a blood sugar reducing effect is prepared by respectively taking eucommia ulmoides, lithocarpus litseifolius and gynura procumbens as raw materials, respectively extracting an eucommia ulmoides extract, a lithocarpus litseifolius extract and a gynura procumbens extract, and then mixing the eucommia ulmoides extract, the lithocarpus litseifolius extract and the gynura procumbens extract according to a mass ratio of 1:1:1-1:1:3 to obtain a combined extract.
Further preferably, the combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobatin and rutin.
A preparation method of a medicinal and edible composition with a hypoglycemic effect comprises the following steps:
step 1): collecting folium Eucommiae as raw material, drying, pulverizing, extracting with water at 80 deg.C at a ratio of 1:10-20 under stirring, filtering, mixing filtrates, concentrating under reduced pressure to 1/10 of the stock solution, adding 4 times of anhydrous ethanol, placing in refrigerator, standing overnight, centrifuging, collecting supernatant, concentrating to no ethanol smell, adding equal amount of distilled water, ultrasonic pulverizing, extracting with solvent of the same volume for three times, mixing extractive phases, and concentrating to dry to obtain folium Eucommiae extract;
step 2): collecting Lithocarpus litseifolius leaves as raw materials, drying, pulverizing, extracting with water at 70 deg.C at a ratio of 1:10-20 under stirring, filtering, mixing filtrates, concentrating under reduced pressure to 1/10 of stock solution, adding 10% active carbon, stirring at 70 deg.C for decolorizing, and spray drying the filtrate to obtain Lithocarpus litseifolius extract;
step 3): collecting folium Notoginseng of Gynura procumbens as raw material, drying, pulverizing, extracting with ethanol water solution, centrifuging, collecting supernatant, concentrating until no alcohol smell is present, and concentrating until dry to obtain folium Notoginseng extract of Gynura procumbens;
step 4): mixing Eucommiae cortex extract, Lithocarpus litseifolius extract and Gynura procumbens extract at a certain proportion, and shaking to obtain medicinal and edible composition.
Further, in the step 4), the eucommia ulmoides extract, the lithocarpus litseifolius extract and the gynura procumbens extract are mixed according to the mass ratio of 1:1:1-1:1:3 to obtain the combined extract.
Further, the combined extract needs to contain chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobatin, rutin and other components.
Further, in the step 3), the extraction solvent is 50-90% ethanol water solution with pH =2-7, the liquid-material ratio is 1:10-20, the extraction temperature is normal temperature, ultrasonic extraction is carried out for 2 hours, and the filtrate is decompressed and concentrated until no alcohol smell exists.
Further, the organic solvent used in step 1) is any one of petroleum ether, n-hexane, dichloromethane, ethyl acetate, n-butanol, dichloromethane/methanol and petroleum ether/ethyl acetate.
Further, preferably, the extraction solvent used in step 1), the selected extraction solvent is ethyl acetate/methanol, and the volume ratio of ethyl acetate/methanol is 4: 1.
The invention also provides application of the prepared medicinal and edible composition in preparing a hypoglycemic medicament.
Due to the adoption of the technical scheme, the invention has the following advantages:
the invention enriches the effective components of eucommia bark, lithocarpus litseifolius and gynura procumbens by different extraction and purification modes to obtain 14 effective components which are respectively chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobatin and rutin, wherein the content of the chlorogenic acid A, the phlorizin, the pinoresinol diglucoside, the isochlorogenic acid C, the trilobatin and the rutin account for 90 percent of the powder of the composition, thereby disclosing the material basis for reducing blood sugar. The prepared medicinal and edible composition has a particularly good effect on patients with diabetes, and the effect is obviously better than that of the extract of eucommia ulmoides, lithocarpus litseifolius and gynura procumbens which are independently used.
Drawings
FIG. 1 is a liquid chromatogram of a composition of the invention.
FIG. 2 is a liquid chromatogram of the Gynura procumbens extract of the present invention.
FIG. 3 is a liquid chromatogram of an extract of eucommia ulmoides Oliver according to the present invention.
FIG. 4 is a liquid chromatogram of the Lithocarpus litseifolius extract of the present invention.
FIG. 5 is a liquid chromatogram of a comparative example extract of the present invention.
Detailed Description
The present invention will be further described in detail with reference to the following examples; however, the following examples are merely illustrative, and the present invention is not limited to these examples.
A preparation method of a medicinal and edible composition with a hypoglycemic effect comprises the following steps:
step 1): collecting folium Eucommiae as raw material, drying, pulverizing, extracting with water at 80 deg.C at a ratio of 1:10-20 under stirring, filtering, mixing filtrates, concentrating under reduced pressure to 1/10 of the stock solution, adding 4 times of anhydrous ethanol, placing in refrigerator, standing overnight, centrifuging, collecting supernatant, concentrating to no ethanol smell, adding equal amount of distilled water, ultrasonic pulverizing, extracting with solvent of the same volume for three times, mixing extractive phases, and concentrating to dry to obtain folium Eucommiae extract;
step 2): collecting Lithocarpus litseifolius leaves as raw materials, drying, pulverizing, extracting with water at 70 deg.C at a ratio of 1:10-20 under stirring, filtering, mixing filtrates, concentrating under reduced pressure to 1/10 of stock solution, adding 10% active carbon, stirring at 70 deg.C for decolorizing, and spray drying the filtrate to obtain Lithocarpus litseifolius extract;
step 3): collecting Notoginseng radix of Gynura procumbens as raw material, drying, pulverizing, extracting with ethanol water as extraction solvent, centrifuging, collecting supernatant, concentrating until no ethanol smell is present, and concentrating until dry to obtain Notoginseng radix extract of Gynura procumbens;
step 4): mixing Eucommiae cortex extract, Lithocarpus litseifolius extract and Gynura procumbens extract at a certain proportion, and shaking to obtain medicinal and edible composition. The combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobatin and rutin by liquid phase detection.
Example 1
Taking 100g of eucommia ulmoides leaves, drying and crushing the eucommia ulmoides leaves, adopting water as an extraction solvent, extracting at the temperature of 80 ℃ and the material-liquid ratio of 1:20, stirring and extracting, filtering, combining filtrate, concentrating the filtrate under reduced pressure to 1/10 of stock solution, adding 4 times of absolute ethyl alcohol, putting the mixture into a refrigerator, standing overnight, centrifuging, collecting supernatant, concentrating the supernatant until no alcohol smell exists, adding equal amount of distilled water for ultrasonic wave scattering, adding equal volume of n-butyl alcohol for extraction, extracting for three times, combining extraction phases, concentrating until the eucommia ulmoides leaves are dried to prepare 2.35g of eucommia ulmoides leaf extract, wherein the yield is 2.35%, and the liquid chromatogram of the eucommia ulmoides extract is shown in figure 3.
Taking 100g of Lithocarpus litseifolius, drying, crushing, taking water as an extraction solvent, extracting at 70 ℃ under stirring with a material-liquid ratio of 1:20, filtering, combining filtrates, concentrating under reduced pressure to 1/10 of a stock solution, adding 10% of active carbon, stirring at 70 ℃ for decolorization, and spray-drying the filtrate to obtain 6.42g of Lithocarpus litseifolius extract with the yield of 6.42%, wherein a liquid chromatogram of the Lithocarpus litseifolius extract is shown in FIG. 4.
Taking 100g of gynura procumbens, drying and crushing, then adopting 50% ethanol water solution with pH =3, the material-liquid ratio is 1:10, the extraction temperature is normal temperature, carrying out ultrasonic extraction for 2 hours, and carrying out reduced pressure concentration on the filtrate until no alcohol smell exists. Concentrating to dry to obtain Gynura procumbens leaf extract 9.24g, with yield 9.24%, and liquid chromatogram of Gynura procumbens extract shown in figure 2.
Mixing the eucommia ulmoides extract, the lithocarpus litseifolius extract and the gynura procumbens extract according to the mass ratio of 1:1:1, and shaking uniformly to prepare the medicine-food homologous composition. The combined extract contains 1 chlorogenic acid, 2 cryptochlorogenic acid, 3 caffeic acid, 4 geniposide, 5 asperuloside, 6 isochlorogenic acid B, 7 catechin, 8 isoquercitrin, 9 isochlorogenic acid A, 10 phloridzin, 11 pinoresinol diglucoside, 12 isochlorogenic acid C, 13 trilobatin and 14 rutin by liquid phase detection, as shown in figure 1.
Example 2
Taking 100g of eucommia ulmoides leaves, drying and crushing the eucommia ulmoides leaves, adopting water as an extraction solvent, stirring and extracting the eucommia ulmoides leaves at the extraction temperature of 80 ℃ and the material-liquid ratio of 1:10, filtering, combining filtrate, concentrating the filtrate under reduced pressure to 1/10 of stock solution, adding 4 times of absolute ethyl alcohol, putting the mixture into a refrigerator, standing overnight, centrifuging, collecting supernatant, concentrating the supernatant until no alcohol smell exists, adding equal amount of distilled water, performing ultrasonic wave scattering, adding equal volume of dichloromethane/methanol for extraction, extracting the mixture for three times, combining extract phases, and concentrating the extract phases until the extract is dried to obtain 4.18g of eucommia ulmoides leaf extract, wherein the yield is 4.;
taking 100g of lithocarpus litseifolius, drying, crushing, taking water as an extraction solvent, extracting at 70 ℃ with a material-liquid ratio of 1:10 under stirring, filtering, combining the filtrates, concentrating under reduced pressure to 1/10 of the stock solution, adding 10% of activated carbon, stirring and decolorizing at 70 ℃, and spray drying the filtrate to obtain 4.25g of lithocarpus litseifolius extract with the yield of 4.25%.
Taking 100g of gynura procumbens pseudo-ginseng leaves, drying and crushing the gynura procumbens pseudo-ginseng leaves, extracting the gynura procumbens pseudo-ginseng leaves by using 80% ethanol water with the pH =2 at the material-liquid ratio of 1:20 at the normal temperature for 2 hours by ultrasonic extraction, and concentrating the filtrate under reduced pressure until no alcohol smell exists. Concentrating to dry to obtain Gynura procumbens leaf extract 8.77g, with yield 8.77%.
Mixing the eucommia ulmoides extract, the lithocarpus litseifolius extract and the gynura procumbens extract according to the mass ratio of 1:1:2, and shaking uniformly to prepare the medicine-food homologous composition. The combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobatin and rutin by liquid phase detection.
Example 3
Taking 100g of eucommia ulmoides leaves, drying and crushing the eucommia ulmoides leaves, adopting water as an extraction solvent, carrying out extraction at 80 ℃ and a material-liquid ratio of 1:15, stirring and extracting, filtering, combining filtrate, concentrating the filtrate under reduced pressure to 1/10 of stock solution, adding 4 times of absolute ethyl alcohol, putting the mixture into a refrigerator, standing overnight, centrifuging, collecting supernatant, concentrating the supernatant until no alcohol smell exists, adding equal amount of distilled water, carrying out ultrasonic wave scattering, adding equal volume of petroleum ether/ethyl acetate for extraction, carrying out extraction for three times, combining extract phases, and concentrating until the mixture is dried to obtain 2.04g of eucommia ulmoides leaf extract, wherein the yield is 2.04%.
Taking 100g of Lithocarpus litseifolius, drying, crushing, taking water as an extraction solvent, extracting at 70 ℃ with stirring, wherein the material-liquid ratio is 1:15, filtering, combining the filtrates, concentrating under reduced pressure to 1/10 of the stock solution, adding 10% of active carbon, stirring and decolorizing at 70 ℃, and spray drying the filtrate to obtain 7.44g of Lithocarpus litseifolius extract with the yield of 7.44%.
Taking 100g of gynura procumbens, drying and crushing, then adopting 60% ethanol water solution with the pH =7, the material-liquid ratio is 1:15, the extraction temperature is normal temperature, ultrasonic extraction is carried out for 2 hours, and the filtrate is decompressed and concentrated until no alcohol smell exists. Concentrating to dry and obtaining the gynura procumbens pseudo-ginseng leaf extract 10.45g with the yield of 10.45 percent.
Mixing the eucommia ulmoides extract, the lithocarpus litseifolius extract and the gynura procumbens extract according to the mass ratio of 1:1:3, and shaking uniformly to prepare the medicine-food homologous composition. The combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobatin and rutin by liquid phase detection.
Comparative example
Taking 100g of each of eucommia ulmoides leaves, lithocarpus litseifolius and gynura procumbens, crushing the materials by using a crusher, adding the crushed materials into a conical flask, extracting the materials by using 90% ethanol water solution with the pH =5 at the material-liquid ratio of 1:15 at normal temperature for 2 hours by ultrasonic extraction, filtering, and concentrating the filtrate under reduced pressure until no alcohol smell exists. Adding 100% ethanol solution 4 times the volume of the obtained solution, placing in a refrigerator at 4 deg.C overnight for alcohol precipitation, centrifuging, collecting supernatant, concentrating under reduced pressure until no alcohol smell exists to obtain mixed extractive solution, extracting the mixed extractive solution with solvent of n-butanol, mixing extractive solutions, concentrating under reduced pressure, and drying to obtain mixed extract 4.52 g containing chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, phlorizin, isochlorogenic acid C and rutin. The compounds of cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, catechin, isoquercitrin, pinoresinol diglucoside and trilobatin were not detected.
Influence of medicinal and edible composition on mouse blood sugar caused by streptozotocin
130 healthy male Kunming mice (about 20 g) were selected as experimental animals and randomly divided into 13 groups of 10 groups of (blank control group, model control group, positive control group (acarbose), mixed extract = 1:1:1 high dose group, mixed extract = 1:1:1 low dose group, mixed extract = 1:1:2 high dose group, mixed extract = 1:1:2 low dose group, mixed extract = 1:1:3 high dose group, mixed extract = 1:1:3 low dose group, Lithocarpus litseifolius extract group, Gynura procumbens extract group, eucommia ulmoides leaf extract group, comparative example group). In the first week, the common feed is selected to provide sufficient food and water so as to avoid the abnormality caused by environmental change. One week later, all mice began a quick water feeding, and 12 hours later, the blank control group was injected with 0.1mol/L of STZ solution intraperitoneally. The injection dose per mouse was selected at 60 mg/kg to model type 2 diabetes, and the blank control group was injected with an equal volume of distilled water. After injection, the placebo mice were fed normal diet and the remaining mice were fed high sugar and high fat diets for 5 days. After 5 days of modeling, mice were tested for blood glucose, which ranged from 11-25mmol/L and was considered successful. If mice with blood glucose values of less than 11 mmol/L are present, additional injections of STZ are required, which are dissolved in citrate buffer at a pH between 4.2 and 4.5. And stored in test tubes wrapped in foil. And injected rapidly into the abdominal cavity of the mice during 30 min. Data results are expressed as mean ± standard deviation (x ± s), and differences between groups are analyzed by one-way variance analysis through statistical software package SPSS 19.0 version, where P <0.05 is considered significant difference and P >0.05 is not significant difference.
The mice with blood sugar concentration within 11-25mmol/L after molding are evenly distributed into 13 groups according to body weight and blood sugar concentration respectively, and the gavage is carried out according to the table 1.
TABLE 1 animal Experimental groups
As shown in table 2, it can be seen from the table that fasting blood glucose levels of diabetic mice significantly increased after successful modeling. And maintained high blood glucose levels, with very significant differences (p <0.01) compared to the blank control group, STZ successfully induced and mimicked the hyperglycemic model of mouse diabetes. The mean blood glucose levels of the blank control group stabilized at 5mmol/L throughout the dosing period, while the mice of the model control group gradually increased with the dosing period. Indicating that the diabetic condition is gradually serious until the fourth and fifth weeks are kept at about 25mmol/L, and the difference between the composition and the low and high dose groups is significant or extremely significant (P <0.01 or P < 0.05).
In positive control and mixed extracts = 1:1:1 high dose group, mixed extract = 1:1:1 low dose group, mixed extract = 1:1:2 high dose group, mixed extract = 1:1:2 low dose group, mixed extract = 1:1:3 high dose group, mixed extract = 1:1: in the 3 low dose group, the blood glucose levels in the first three weeks of mice increased with increasing treatment period. However, it was slower than the model control group. From week 4, the positive control group significantly reduced fasting blood glucose levels in diabetic mice; after five weeks of gastric lavage, the blood sugar of the positive control group mice is reduced by 45.54 percent compared with the blood sugar of the model control group mice, and the significant difference exists.
Mixed extract = 1:1:1 high dose group, mixed extract = 1:1:2 high dose group, mixed extract = 1:1: the fasting blood glucose values of the mice in the 3 high-dose groups are reduced in different degrees from the fourth week of the gavage, and show a significant or very significant difference compared with the model control group ((P <0.01 or P <0.05), and after 5 weeks of the gavage, the blood glucose of the mice in the mixed extract = 1:1:1 high-dose group, the blood glucose of the mice in the mixed extract = 1:1:2 high-dose group and the blood glucose of the mice in the mixed extract = 1:1:3 high-dose group is reduced by 80% on average relative to the blood glucose of the mice in the model group, so that the composition can have a certain control effect on diabetes.
The lithocarpus litseifolius extract group, the gynura procumbens extract group and the eucommia ulmoides leaf extract group show the same trend in fasting blood glucose value of mice in the comparative example group and the high and low dose groups of the mixed extract, except that the lithocarpus litseifolius extract group, the gynura procumbens extract group and the eucommia ulmoides leaf extract group show poorer effects in the fourth and fifth weeks than the mixed extract group, but the lithocarpus litseifolius extract group, the gynura procumbens extract group and the eucommia ulmoides leaf extract group have smaller fasting blood glucose values in the fourth and fifth weeks than the comparative example group, which indicates that the mixed extract effect is better than that of the extracts of the three independent medicinal and edible plants under the same dose and the comparative example has the worst effect.
TABLE 2 Effect of Mixed extracts on blood glucose levels in diabetic mice
Compared with the normal control group, the composition has the advantages that,##𝑃<0.01; in comparison to the set of models,*𝑃<0.05, **𝑃<0.01 and***𝑃<0.001。
the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (5)
1. A preparation method of a medicinal and edible composition with a hypoglycemic effect is characterized by comprising the following steps:
step 1): collecting folium Eucommiae as raw material, drying, pulverizing, extracting with water at 80 deg.C at a liquid-to-material ratio of 1:10-20 under stirring, filtering, mixing filtrates, concentrating under reduced pressure to 1/10 of the stock solution, adding 4 times of anhydrous ethanol, placing in refrigerator, standing overnight, centrifuging, collecting supernatant, concentrating to no alcohol smell, adding equal amount of distilled water, ultrasonic pulverizing, adding equal volume of solvent, extracting for three times, mixing extractive phases, and concentrating to dry to obtain folium Eucommiae extract;
step 2): collecting Lithocarpus litseifolius leaves as raw materials, drying, pulverizing, extracting with water at 70 deg.C at a liquid-to-material ratio of 1:10-20 under stirring, filtering, mixing filtrates, concentrating under reduced pressure to 1/10 of the stock solution, adding 10% active carbon, stirring at 70 deg.C for decolorizing, and spray drying the filtrate to obtain Lithocarpus litseifolius extract;
step 3): collecting Notoginseng radix of Gynura procumbens as raw material, drying, pulverizing, extracting with ethanol water as extraction solvent, centrifuging, collecting supernatant, concentrating until no ethanol smell is present, and concentrating until dry to obtain Notoginseng radix extract of Gynura procumbens;
step 4): mixing the eucommia ulmoides extract, the lithocarpus litseifolius extract and the gynura procumbens extract according to the mass ratio of 1:1:1-1:1:3, and shaking uniformly to prepare the medicinal and edible composition.
2. The method for preparing a pharmaceutical and edible composition with hypoglycemic effect as claimed in claim 1, wherein the combined extract contains chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, asperuloside, isochlorogenic acid B, catechin, isoquercitrin, isochlorogenic acid A, phlorizin, pinoresinol diglucoside, isochlorogenic acid C, trilobatin and rutin.
3. The preparation method of the medicinal and edible composition with the hypoglycemic effect according to claim 1, wherein in the step 3), the extraction solvent is 50-90% ethanol aqueous solution with pH =2-7, the liquid-material ratio is 1:10-20, the extraction temperature is normal temperature and ultrasonic extraction is performed for 2 hours, and the filtrate is concentrated under reduced pressure until no alcohol smell exists.
4. The preparation method of the medicinal and edible composition with hypoglycemic effect according to claim 1, wherein the organic solvent used in step 1) is any one of petroleum ether, n-hexane, dichloromethane, ethyl acetate, n-butanol, dichloromethane/methanol and petroleum ether/ethyl acetate.
5. Use of the medicinal and edible composition prepared according to any one of claims 1-4 in the preparation of hypoglycemic drugs.
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