CN104958582A - Lophatherum gracile extract product, preparation containing extract product, and use of extract product - Google Patents
Lophatherum gracile extract product, preparation containing extract product, and use of extract product Download PDFInfo
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Abstract
The invention discloses a Lophatherum gracile extract product, a preparation containing the extract product, and a use of the extract product, belongs to the field of traditional Chinese medicines, and concretely relates to a Lophatherum gracile extract product and a medicinal preparation containing the extract product. The Lophatherum gracile extract product can be used for preparing medicines for preventing or treating obesity. The extract product is prepared through the following steps: adding a small amount of a dried orange peel medicinal material to a Lophatherum gracile medicinal material, extracting, removing impurities and enriching active components, has an obvious obesity treatment effect, and has a wonderful effect than a Lophatherum gracile medicinal material individually extracted product. The Lophatherum gracile extract product with a substantial treatment effect can be obtained through extracting by using an appropriate concentration of an ethanol-water solution, removing impurities and enriching on the basis of full and reasonable test design. The invention also provides an appropriate liquid phase analysis method used for controlling the difference among batches of the extract product prepared from different producing areas and batches of medicinal materials.
Description
Technical field
The present invention relates to the field of Chinese medicines, be specifically related to a kind of Herba Lophatheri extract and containing the pharmaceutical preparation of this extract, this Herba Lophatheri extract can be used for preparing the medicine of prevention or treatment of obesity.
Background technology
Plant Herba Lophatheri (Herba Loophatheri) has another name called Rhizoma lophantheri, Gallus jabouillei rice, Herba Lophatheri, for grass family Herba Lophatheri belongs to herbaceos perennial.Expand for spindle meat tuber in the middle part of Herba Lophatheri fibrous root, yellow-white; Leaf lanceolar, wide 2 ~ 3cm, vein is parallel, has obvious small crossvein, and blade face is without hair or have seta, and there is short bristle at edge; Panicle top is raw, and small ear is bordering on stockless, and arrangement is cob side slightly partially, wholely comes off; Caryopsis is oval.Herba Lophatheri is mainly born in the dark and damp place of hillside sylvan life, is distributed in each provinces and regions on the south the Changjiang river.Herba Lophatheri has effect of clearing heat and relieving fidgetness, diuresis, be mainly used in treating in the heart disease heat, cough with dyspnea, haematemesis, pyretic toxicity wind, only quench one's thirst, press borax poison, expectorant, control fanatical unhappiness, apoplectic aphasia in silence, pain wind syndrome of head, spasmolytic are throbbed with fear, pestilence fan is vexed, kill small worm, heat extraction delays the symptoms such as spleen.The dry stem and leaf that medical material Herba Lophatheri (Lophatherum gracile Brongn.) is grass family (Gramineae) herbaceous plant Herba Lophatheri (Lophatherum gracile Brongn.), has clearing away heat-fire, relieving restlessness is quenched the thirst, effect of inducing diuresis for treating stranguria syndrome.Ming Dynasty's Li Shizhen (1518-1593 A.D.) claims it in Compendium of Material Medica: sweet, cold, nontoxic, remove dysphoria with smothery sensation, diuresis, clear away heart-fire.
Containing a large amount of flavone compounds and bioactive polysaccharide and other effective ingredient in Herba Lophatheri, as phenolic acid compound, anthraquinone analog compound, terpene lactone, extraordinary aminoacid and active skin, manganese, zinc, selenium and other trace elements.Functional factor mainly flavone glycoside and Coumarins lactone contained in Herba Lophatheri.The content of its effective ingredient and biological activity all have comparability with Folium Ginkgo.
Modern pharmacological research shows, reactive oxygen free radical in functional factor energy purged body contained in Herba Lophatheri; The activity of the Antioxidant Enzymes of induction organism inside; The anti-stress of enhancing body and anti-fatigue ability; Improve memory ability, the process etc. of slow down aging.In addition, also abundant chlorophyll is contained in Herba Lophatheri.Research shows, chlorophyll is the important component of many vegetable antimutagenesises, has the anti-canceration effect of antitumor, and because chlorophyll has stronger anti-oxidation function, this is to prevention cardiovascular diseases and anti-agingly also have positive role.
Summary of the invention
The object of the present invention is to provide a kind of Herba Lophatheri extract, the preparation method of this extract and liquid phase analysis method, the pharmaceutical preparation containing this extract and utilize this extract to prepare prevention or the purposes of obesity treating medicine.
Above-mentioned purpose of the present invention is achieved by technical scheme below:
A kind of Herba Lophatheri extract, this extract is prepared by following methods:
Step one: Herba Lophatheri medical material and weight are the Pericarpium Citri Reticulatae medical material co-grinding of Herba Lophatheri medical material 0.05 ~ 0.09 times;
Step 2: be the ethanol water circumfluence distillation 2 ~ 3 hours of 60% ~ 85% by the medical material concentration of pulverizing, extract 2 ~ 3 times, each ethanol water volume is Herba Lophatheri medical material weight 3 ~ 5 times;
Step 3: be concentrated into by gained extracting solution without alcohol taste, by D101 type macroporous resin enrichment, first uses 30% ~ 40% ethanol remove impurity, be eluted to eluent colourless, then use 65% ~ 85% ethanol deposition activity part, be eluted to eluent colourless, 65% ~ 85% ethanol elution is concentrated, spraying dry.
Preferably, in described preparation method, Pericarpium Citri Reticulatae medical material weight is 0.06 ~ 0.08 times of Herba Lophatheri medical material weight.
Preferably, in described preparation method, described in step 2, ethanol water is the ethanol water of 75%.
Preferably, in described thing preparation method, step 3 is used for the ethanol of remove impurity is 35% ethanol.
Preferably, in described preparation method, step 3 is used for the ethanol of deposition activity component is 70% ethanol.
In order to be controlled Herba Lophatheri extract quality prepared by Different sources, batch medical material by the method setting up HPLC finger printing, the liquid phase analysis method of described extract is:
Chromatographic column: Agilent Extend C18 post (4.6mm × 250mm, 5 μm);
Mobile phase: A is acetonitrile, and B is 0.1% phosphoric acid solution (triethylamine adjust ph to 5.5);
Gradient elution program: 0.01 ~ 70min, A 20% → 80%;
Flow rate of mobile phase: 1.0mLmin
-1;
Determined wavelength: 230nm; Column temperature: 25 DEG C; Sample size: 10 μ L.
Pharmaceutical preparation, the described Herba Lophatheri extract containing treatment effective dose and pharmaceutically acceptable carrier.
The application of described Herba Lophatheri extract in preparation prevention or obesity treating medicine.
When extract of the present invention is used as medicine, directly can uses, or use in the form of a pharmaceutical preparation.
Described pharmaceutical preparation contains the Herba Lophatheri extract of the present invention for the treatment of effective dose, and all the other are acceptable on materia medica, nontoxic to humans and animals and pharmaceutically suitable carrier of inertia and/or excipient.
Described pharmaceutically suitable carrier or excipient are that one or more are selected from solid, semisolid and liquid diluent, filler and pharmaceutical preparation adjuvant.Pharmaceutical preparation of the present invention is used with the form of per weight dose.Extract of the present invention is applied to by form that is oral or injection the patient needing treatment.For time oral, tablet, slow releasing tablet, controlled release tablet, capsule, drop pill, micropill, suspensoid, Emulsion, powder or granule, oral liquid etc. can be made into; During for injecting, can be made into aqueous or oily solution, aseptic powder injection, liposome or the Emulsion etc. of sterilizing.
Advantage of the present invention: the present invention, by adding a small amount of Pericarpium Citri Reticulatae medical material in Herba Lophatheri medical material, makes final obtained extract have the effect that significantly treatment is fat, than having an unexpected effect with Herba Lophatheri medicinal material extract tool separately; The present invention is based on fully, rational EXPERIMENTAL DESIGN, to extract by selecting the ethanol water of suitable concn to carry out, remove impurity and enrichment, the significant Herba Lophatheri extract of therapeutical effect can be obtained; Liquid phase analysis method provided by the invention may be used for the finger printing setting up this extract, for controlling Different sources, the quality of extract prepared by batch medical material.
Accompanying drawing explanation
Fig. 1 is extractive HPLC chromatograms.
Detailed description of the invention
Further illustrate essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.Although be explained in detail the present invention with reference to preferred embodiment, those of ordinary skill in the art should be appreciated that and can modify to technical scheme of the present invention or equivalent replacement, and does not depart from essence and the scope of technical solution of the present invention.
Main agents and material: Herba Lophatheri medical material and Pericarpium Citri Reticulatae medical material are purchased from Haozhou, Anhui Chinese Medicinal Materials Markets; Food-grade ethanol is purchased from Shanghai Ling Feng chemical reagent company limited; Pharmaceutical grade D101 macroporous resin is purchased from sky tunami letter resin company limited; Acetonitrile is HPLC level, is purchased from TEDIA; 85% phosphoric acid is HPLC level, is purchased from TEDIA; Chromatographic grade pure water is heartily pure water.
Embodiment 1: Pericarpium Citri Reticulatae medical material amount is Herba Lophatheri weight 0.05 times
Get 10kg Herba Lophatheri medical material and 0.5kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 2: Pericarpium Citri Reticulatae medical material amount is Herba Lophatheri weight 0.06 times
Get 10kg Herba Lophatheri medical material and 0.6kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 3: Pericarpium Citri Reticulatae medical material amount is Herba Lophatheri weight 0.08 times
Get 10kg Herba Lophatheri medical material and 0.8kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 4: Pericarpium Citri Reticulatae medical material amount is Herba Lophatheri weight 0.09 times
Get 10kg Herba Lophatheri medical material and 0.9kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 5: step 2 60% ethanol water extracts
Get 10kg Herba Lophatheri medical material and 0.6kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 60%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 6: step 2 85% ethanol water extracts
Get 10kg Herba Lophatheri medical material and 0.6kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 85%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 7: step 3 is except using mixedly 30% ethanol
Get 10kg Herba Lophatheri medical material and 0.6kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 30% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 8: step 3 is except using mixedly 40% ethanol
Get 10kg Herba Lophatheri medical material and 0.6kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 40% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol deposition activity part, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 9: step 3 enrichment 65% ethanol
Get 10kg Herba Lophatheri medical material and 0.6kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 65% ethanol deposition activity part, be eluted to eluent colourless, 65% ethanol elution concentrated, spraying dry.
Embodiment 10: step 3 enrichment 85% ethanol
Get 10kg Herba Lophatheri medical material and 0.6kg Pericarpium Citri Reticulatae medical material co-grinding; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 85% ethanol deposition activity part, be eluted to eluent colourless, 85% ethanol elution concentrated, spraying dry.
Embodiment 11: comparative example's (not adding Pericarpium Citri Reticulatae)
Get 10kg Herba Lophatheri pulverizing medicinal materials; With the ethanol water circumfluence distillation 3 times that concentration is 75%, first time 50L extracts 3 hours, and second and third time 30L extracts 2 hours; Gained extracting solution is concentrated into without alcohol taste, by D101 type macroporous resin enrichment, first uses 35% ethanol remove impurity, be eluted to eluent colourless, then use 70% ethanol enrichment, be eluted to eluent colourless, 70% ethanol elution concentrated, spraying dry.
Embodiment 12: liquid-phase chromatographic analysis
Need testing solution is prepared: in the brown volumetric flask of extract 5mg to 50mL that Example 2 method is obtained, add 30mL 20% acetonitrile solution ultrasonic dissolution, after being cooled to room temperature, continue to add 20% acetonitrile solution standardize solution.
Analytical method:
High performance liquid chromatograph: Agilent 1260, binary pump;
Chromatographic column: Agilent Extend C18 post (4.6mm × 250mm, 5 μm);
Mobile phase: A is acetonitrile, and B is 0.1% phosphoric acid solution (triethylamine adjust ph to 5.5);
Gradient elution program: 0.01 ~ 70min, A 20% → 80%;
Flow rate of mobile phase: 1.0mLmin
-1;
Determined wavelength: 230nm; Column temperature: 25 DEG C; Sample size 10 μ L.
Analyze the extract prepared with 10 batches of Different sources, batch Herba Lophatheri and Pericarpium Citri Reticulatae, set up finger printing and mate, 1 ~ No. 9 peak all occurs in 10 batch sample chromatograms as a result.Therefore demarcate 9 peaks for total fingerprint peaks, set up the HPLC finger printing of this extract accordingly, the results are shown in Figure 1.
Embodiment 13: the preparation of tablet
By embodiment 2 method first obtained extract, be 1 by itself and excipient weight ratio
:the ratio of 8 adds excipient, pelletizing press sheet.
Embodiment 14: the preparation of oral liquid
By embodiment 2 method first obtained extract, oral liquid method for making makes oral liquid routinely.
Embodiment 15: the preparation of capsule or granule
By embodiment 2 method first obtained extract, be 1 by itself and excipient weight ratio
:the ratio of 9 adds excipient, makes capsule or granule.
Embodiment 16: the preparation of injection
Obtain extract by embodiment 2 method, inject with water, fine straining, injection is made in embedding sterilizing.
Embodiment 17: the preparation of aseptic powder injection
By embodiment 2 method first obtained extract, be dissolved in sterile water for injection, stirring makes molten, filters with aseptic suction funnel, more aseptic fine straining, and be sub-packed in ampoule, after frozen drying, aseptic sealing by fusing obtains injectable powder.
Embodiment 18: Herba Lophatheri extract measures the inhibit activities of pancreatic lipase
First substrate p-Nitrophenyl acetate (sigma company) phosphate buffer (PBS, pH7.4) is made into 1.35M; Porcine pancreatic lipase (sigma company) is made into 10mg/mL with phosphate buffer; The Herba Lophatheri extract phosphate buffer obtained according to the method for embodiment 2 is mixed with the solution of variable concentrations.Then add successively in 96 orifice plates 50 μ L dilute 20 times after enzymatic solution, 40 μ L dilute the given the test agent of the substrate solution after 1000 times and 10 μ L variable concentrations, mixing.React 20 minutes at 25 DEG C, under 405nm, detected the absorbance in every hole every 2 minutes.
Calculating given the test agent to the suppression ratio (%) of pancreatic lipase according to the absorbance under 405nm, take distilled water as contrast, and when inhibition of enzyme activity rate (%) being reached 50%, the concentration determination of inhibitor is IC
50value.Suppression ratio (%) calculates according to following formula:
Suppression ratio (%)=[(A-B)-(C-D)]/(A-B) × 100%
In above formula, A represent reaction after the absorbance of blank well under 405nm, B represent reaction before the absorbance of blank well under 405nm, C represent reaction after the absorbance of sample well under 405nm, D represent reaction before the absorbance of sample well under 405nm.
Detect Herba Lophatheri extract to the inhibitory action result of pancreatic lipase, IC
50be 10.65 ± 0.139 μ g/mL, show that Herba Lophatheri extract has significant inhibitory action to pancreatic lipase.
Embodiment 19: Herba Lophatheri extract is to the antiobesity action of obese rat
Zucker rat (heritability obese rat) is bred by Shanghai medicine institute of Chinese Academy of Sciences Animal House, male, 8 week age.Get normal Zucker rat 6, as blank group.Fatty Zucker rats is divided into 12 groups, i.e. model control group, Herba Lophatheri extract group (totally 10 groups) and comparative example's group, often organizes 6.By Herba Lophatheri extract 0.5%CMC-Na hydrotropy obtained for the method according to embodiment 2, dosage is 1g/kg; Blank and model control group gives the 0.5%CMC-Na of equal volume, oral administration gavage administration two weeks.Periodic detection diet and body weight.The results are shown in Table 1.
Table 1 Herba Lophatheri extract is on the impact (± SE) of Zucker rat body weight and diet
Compared with model control group,
*p < 0.05.
Conclusion: as can be seen from Table 1, compared with model control group, Herba Lophatheri extract group rat body weight of the present invention increases and obviously declines, and do not have a significant impact, and rat feces is normal to diet.
Fat intake too much may be causeed fat and the decompensated disease such as the diabetes relevant to obesity, hyperlipemia, fatty liver.Suppress pancreatic lipase that fat can be suppressed in the decomposition of small intestinal, thus suppress the absorption of fat.Herba Lophatheri extract as pancreatic lipase inhibitor, and has antiobesity action, can be used for the diseases such as prevention or treatment of obesity.
Claims (8)
1. a Herba Lophatheri extract, is characterized in that described extract is prepared by following methods:
Step one: Herba Lophatheri medical material and weight are the Pericarpium Citri Reticulatae medical material co-grinding of Herba Lophatheri medical material 0.05 ~ 0.09 times;
Step 2: be the ethanol water circumfluence distillation 2 ~ 3 hours of 60% ~ 85% by the medical material concentration of pulverizing, extract 2 ~ 3 times, each ethanol water volume is Herba Lophatheri medical material weight 3 ~ 5 times;
Step 3: be concentrated into by gained extracting solution without alcohol taste, by D101 type macroporous resin enrichment, first uses 30% ~ 40% ethanol remove impurity, be eluted to eluent colourless, then use 65% ~ 85% ethanol deposition activity part, be eluted to eluent colourless, 65% ~ 85% ethanol elution is concentrated, spraying dry.
2. Herba Lophatheri extract according to claim 1, is characterized in that: Pericarpium Citri Reticulatae medical material weight is 0.06 ~ 0.08 times of Herba Lophatheri medical material weight.
3. Herba Lophatheri extract according to claim 1, is characterized in that: ethanol water described in step 2 is the ethanol water of 75%.
4. Herba Lophatheri extract according to claim 1, is characterized in that: the ethanol that step 3 is used for remove impurity is 35% ethanol.
5. Herba Lophatheri extract according to claim 1, is characterized in that: the ethanol that step 3 is used for deposition activity component is 70% ethanol.
6. Herba Lophatheri extract according to claim 1, is characterized in that: the liquid phase analysis method of described extract is:
Chromatographic column: Agilent Extend C18 post (4.6mm × 250mm, 5 μm);
Mobile phase: A is acetonitrile, and B is 0.1% phosphoric acid solution (triethylamine adjust ph to 5.5);
Gradient elution program: 0.01 ~ 70min, A 20% → 80%;
Flow rate of mobile phase: 1.0mLmin
-1;
Determined wavelength: 230nm; Column temperature: 25 DEG C; Sample size: 10 μ L.
7. pharmaceutical preparation, is characterized in that: described preparation contains the Herba Lophatheri extract according to claim 1 for the treatment of effective dose and pharmaceutically acceptable carrier.
8. the application of Herba Lophatheri extract as claimed in claim 1 in preparation prevention or treatment antiobesity agents.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109374785A (en) * | 2018-12-21 | 2019-02-22 | 广东方制药有限公司 | The construction method and detection method of lophatherum gracile medicinal material UPLC characteristic spectrum |
| CN112587620A (en) * | 2020-12-10 | 2021-04-02 | 江西农业大学 | Application of herba Pteridis Multifidae extract in preparing weight-reducing composition and weight-reducing composition |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302685A (en) * | 2011-05-13 | 2012-01-04 | 暨南大学 | Common lophatherum herb extract and preparation method and application thereof |
| KR101302222B1 (en) * | 2012-09-18 | 2013-08-30 | 주식회사 아가방앤컴퍼니 | Mixed vegetable extracts for preventing, improving and treating atopic dermatitis and allergic skin diseases, composition including the same and method of producing the same |
| CN103601775A (en) * | 2013-11-27 | 2014-02-26 | 桂林益元素生物科技有限公司 | Method for extracting hesperidin from orange peels |
| CN103845477A (en) * | 2012-12-04 | 2014-06-11 | 浙江中医药大学 | Pericarpium citri reticulatae extract with antihyperlipidemic effect and application thereof |
-
2015
- 2015-06-09 CN CN201510312272.XA patent/CN104958582A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302685A (en) * | 2011-05-13 | 2012-01-04 | 暨南大学 | Common lophatherum herb extract and preparation method and application thereof |
| KR101302222B1 (en) * | 2012-09-18 | 2013-08-30 | 주식회사 아가방앤컴퍼니 | Mixed vegetable extracts for preventing, improving and treating atopic dermatitis and allergic skin diseases, composition including the same and method of producing the same |
| CN103845477A (en) * | 2012-12-04 | 2014-06-11 | 浙江中医药大学 | Pericarpium citri reticulatae extract with antihyperlipidemic effect and application thereof |
| CN103601775A (en) * | 2013-11-27 | 2014-02-26 | 桂林益元素生物科技有限公司 | Method for extracting hesperidin from orange peels |
Non-Patent Citations (8)
| Title |
|---|
| 刘明等: "大孔吸附树脂精制纯化淡竹叶总黄酮的研究", 《中国民族民间医药》 * |
| 初阳等: "淡竹叶中竹叶黄酮的纯化工艺研究", 《医药导报》 * |
| 谢捷等: "竹叶黄酮和香豆素类内酯的提取及含量比较", 《林业实用技术》 * |
| 郑里翔: "《生物化学》", 31 January 2015, 中国医药科技出版社 * |
| 郭宏昌: "《中草药抗肿瘤便览》", 30 November 2014, 新疆人民卫生出版社 * |
| 金旭丹: "《神奇的元素》", 31 January 2007, 内蒙古人民出版社 * |
| 闻华工作室茶疗健康组: "《肾病药茶》", 30 June 2005, 广东科技出版社 * |
| 黄世敬: "《轻松降血脂》", 31 March 2015, 金盾出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109374785A (en) * | 2018-12-21 | 2019-02-22 | 广东方制药有限公司 | The construction method and detection method of lophatherum gracile medicinal material UPLC characteristic spectrum |
| CN112587620A (en) * | 2020-12-10 | 2021-04-02 | 江西农业大学 | Application of herba Pteridis Multifidae extract in preparing weight-reducing composition and weight-reducing composition |
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Application publication date: 20151007 |