CN1919229B - Bauhinia hupehara C. and its extract for the treatment of diabetes and obesity - Google Patents
Bauhinia hupehara C. and its extract for the treatment of diabetes and obesity Download PDFInfo
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- CN1919229B CN1919229B CN 200510093358 CN200510093358A CN1919229B CN 1919229 B CN1919229 B CN 1919229B CN 200510093358 CN200510093358 CN 200510093358 CN 200510093358 A CN200510093358 A CN 200510093358A CN 1919229 B CN1919229 B CN 1919229B
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- radix bauhiniae
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- bauhiniae hupehanae
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Abstract
The invention relates to an extract obtained from Bauhinia hupehara C, a Chinese medicinal herb and its use in lowering blood sugar and fat-reducing. The extract comprises flavones (including flavone glycosides), saponin and polyphenol acid. The extract can be used for treating diabetes and obesity.
Description
Technical field
The present invention relates to Radix Bauhiniae Hupehanae (Bauhinia hupehara C has another name called Hubei Province Bauhinia purpurea), extract, its preparation method, contain this extract compositions and, Radix Bauhiniae Hupehanae or its extract be used for the product purpose of prevention and treatment diabetes and obesity.
Background technology
Radix Bauhiniae Hupehanae (Bauhinia hupehara C) belongs to the pulse family bauhinia.Main effect is treatment dysentery, painful and swollen testis and scrotum pruritus due to wind pathogen etc.Bibliographical information contain 5,7-dihydroxy chromone, aromadendrin and dihydroquercetin (CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1992,17 (10): 613-5) in the Radix Bauhiniae Hupehanae.
Summary of the invention
Inventor's existing plant Radix Bauhiniae Hupehanae (Bauhiniahupehara) whole plant or its extract unexpectedly found under study for action has strong blood sugar lowering and reduces the body weight effect, thereby can be used for treating diabetes and obesity.
The present invention relates to Radix Bauhiniae Hupehanae plant or its extract for the preparation of prevention or slow down or treat purposes in diabetes and the obesity product, be particularly useful for treating diabetes.
The present invention relates to a kind of method for preparing the Radix Bauhiniae Hupehanae extract on the other hand, and it comprises Radix Bauhiniae Hupehanae vegetable material water, organic solvent or water-containing organic solvent extraction or leach the Radix Bauhiniae Hupehanae vegetable material, obtains the Radix Bauhiniae Hupehanae extract.
" extract or leach " of mentioning in the method among the present invention said for example: extraction or leaching modes such as decocting method, infusion process, percolation or steam distillation.
In extracting method of the present invention, if necessary, also comprise extracting solution or leachate are concentrated.A kind of common method for concentration is evaporation, for example atmospheric evaporation, reduction vaporization, thin film evaporation etc.Can carry out drying after concentrating, for example, constant pressure and dry, drying under reduced pressure, spray drying, lyophilization etc.Dry optional step, but be preferred in certain embodiments.
In some embodiment, concentration step also can omit.For example, can directly use leachate, for example directly take to the patient, perhaps directly contain the compositions of Radix Bauhiniae Hupehanae extract as feedstock production.
Extract of the present invention (as leachate or concentrated, dry extractum afterwards) can also further carry out purification.This can adopt any or several the carrying out in many purification process known in the art.In purge process, preferably monitor the existence of active component.For example by blood sugar lowering or reduce body weight or active component is monitored in treatment diabetes or treatment of obesity.
In the methods of the invention, described Radix Bauhiniae Hupehanae (Bauhinia hupehara C) vegetable material can be Radix Bauhiniae Hupehanae plant Herb or its any part, and for example its organ, tissue or cell comprise seed, stem, leaf, root, the preferably aerial parts of Radix Bauhiniae Hupehanae plant, for example stem and leaf.The Radix Bauhiniae Hupehanae vegetable material can be medical material, also can be crude drug.The Radix Bauhiniae Hupehanae vegetable material can be tissue culture, cell culture.The Radix Bauhiniae Hupehanae plant can be natural or genetically modified.The Radix Bauhiniae Hupehanae vegetable material can be drying or fresh.
In extracting method of the present invention, described Radix Bauhiniae Hupehanae vegetable material can be through pretreated.These processing comprise physical treatment, chemical treatment, biological treatment etc.For example, the Radix Bauhiniae Hupehanae vegetable material can simply wash in advance, pulverize.
In the present invention, organic solvent can be methanol, ethanol, propanol, acetone, ethyl acetate or n-butyl alcohol or their any combination.Described " water-containing organic solvent " is the aqueous solution that contains organic solvent; Wherein organic solvent can be methanol, ethanol, propanol, acetone or n-butyl alcohol or their any combination.Water can be for example distilled water, ion exchange water or tap water.
" product " described in the present invention says it can is any product that contains Radix Bauhiniae Hupehanae vegetable material or its extract for example, as functional food, and health product, or medicine etc.
In a specific embodiments, the preparation method of Radix Bauhiniae Hupehanae plant extract of the present invention be aerial parts with the Radix Bauhiniae Hupehanae plant under room temperature, normal pressure, obtain Radix Bauhiniae Hupehanae with moisture organic solvent lixiviate and extract extractum.Crude drug Radix Bauhiniae Hupehanae and immersion are recommended as 1 with the w/v of the solution of water-containing organic solvent: 5-15 (kg/liter).Immersion or decocting time were recommended greater than 1 hour, and soaking temperature is recommended 0 ℃-60 ℃, decocted temperature and recommended 90 ℃-100 ℃.Used organic solvent includes but not limited to methanol, ethanol, propanol, acetone, ethyl acetate and n-butyl alcohol.Concentrating can be with the extract concentrating under reduced pressure, and temperature is recommended to be equal to or less than 60 ℃ and carried out.
The invention still further relates to the Radix Bauhiniae Hupehanae extract.Radix Bauhiniae Hupehanae extract of the present invention refers to the Radix Bauhiniae Hupehanae extract that extracts by water, organic solvent or water-containing organic solvent from the Radix Bauhiniae Hupehanae vegetable material, but this Radix Bauhiniae Hupehanae extract blood sugar lowering or reduce body weight or the active component for the treatment of diabetes or treatment of obesity.
Extract of the present invention can obtain by the extracting method of the present invention of above definition.
Again on the one hand, the present invention relates to can be by the Radix Bauhiniae Hupehanae extract of any method acquisition of the present invention.
For example, can pass through decocting method, infusion process, percolation or steam distillation etc. and extract (perhaps leaching) Radix Bauhiniae Hupehanae vegetable material, obtain leachate.Then, randomly concentrate, dry or purification or their any combination.
Gained extractum or purified product can further be mixed with the purpose compositions, as pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
Mainly contain compositions such as flavone (comprising flavonoid glycoside), saponin, stilbene and polyphenol acid in the extract of the present invention.
Extract of the present invention has blood sugar lowering and weight losing function, and the medicine that it can be used for preparing treatment diabetes and obesity comprises oral medicine and injection, also can be prepared as the health product of blood sugar lowering, fat-reducing.
On the other hand, the present invention relates to contain the compositions of Radix Bauhiniae Hupehanae of the present invention or its extract.Preferably, said composition also contains excipient or carrier.
In specific embodiments, described compositions is pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion.
It is known in the art that Radix Bauhiniae Hupehanae extract of the present invention is mixed with the purpose method for compositions.According to different needs, one of ordinary skill in the art of the present invention can select suitable additive, compound method, dosage form, unit dose etc.
When the present composition is pharmaceutical composition, can contain suitable pharmaceutical excipient, carrier or diluent.Can adopt the pharmaceutical field technique known to carry out preparation.
Pharmaceutical composition of the present invention can adopt multiple dosage form, and this often depends on route of administration.For example, Radix Bauhiniae Hupehanae extract of the present invention can be used for preparing the medicine for the treatment of diabetes and obesity, comprises the preparation that oral medicine and parenteral are used, as injection.
Pharmaceutical composition of the present invention can pass through the number of ways administration, for example oral, parenteral etc.Dosage regimen mediating recipe amount depends on multiple factor, as route of administration, patient health situation etc., and can be determined by the doctor.On the other hand, the compositions that the present invention relates to the Radix Bauhiniae Hupehanae extract or contain the Radix Bauhiniae Hupehanae extract for the preparation of blood sugar lowering or reduce body weight or the medicine for the treatment of diabetes or treatment of obesity in purposes.
On the other hand, the present invention relates to Radix Bauhiniae Hupehanae vegetable material or its extract (preferred water extract) for the production of the purposes in the compositions that can be used for blood sugar lowering or reduction body weight or treatment diabetes or treatment of obesity.Described compositions is pharmaceutical composition, health composition, food compositions or beverage composition for treating dental erosion preferably.
On the other hand, the present invention relates to a kind of blood sugar lowering or reduce body weight or the method for the treatment of diabetes or treatment of obesity, comprise to the patient giving Radix Bauhiniae Hupehanae vegetable material or its extract or containing the compositions of this extract or other product after by the processing of Radix Bauhiniae Hupehanae vegetable material.
" patient " comprises any animal, preferred mammal, particularly house pet, letting animals feed.A preferred embodiment this, the patient is the people." patient " suffers from or suspects the individuality of suffering from diabetes or obesity or relevant disease, and also can be has the individuality of suffering from obesity or risk of diabetes or relevant disease, or normal individual.Therefore, Radix Bauhiniae Hupehanae vegetable material of the present invention or its extract or contain the compositions of this extract or the purposes of other product after by the processing of Radix Bauhiniae Hupehanae vegetable material not only comprises treatment also comprise prevent diabetes or obesity or relevant disease.
The specific embodiment
Of the present invention further specifying will further specify at embodiment, but they should not be construed as and limit the scope of the present invention.
Embodiment 1
Radix Bauhiniae Hupehanae (Bauhinia hupehara C) aerial parts 1kg pulverizes, and decocts 2h with 85% ethanol 5L normal pressure, filters, and residue continues to decoct 1h with 85% ethanol 5L.Filter, merging filtrate, 55 ℃ of concentrating under reduced pressure obtain the Radix Bauhiniae Hupehanae extractum 171g of alcohol extraction, yield 17.1%.
Embodiment 2
The extract of embodiment 1 adopts hydrochloric acid-magnesium powder method of inspection to differentiate and is positive, and shows and contains flavone compound; Acetic anhydride-strong sulfuric acid response is positive, and shows to contain saponins compound; 1% ferric chloride reagent reacting and vanillin-hydrochloric acid reagent reaction is positive, shows to contain phenols and tannin compounds; The alpha-Naphthol strong sulfuric acid response is positive, and shows to contain the reducing sugar glycosides compound.
Embodiment 3
50 kunming mices are divided into normal control group (10) at random and treat modeling group (40).Treat that the fasting of modeling group mice (can't help water) after 24 hours, tail vein fast injection alloxan normal saline solution, dosage is 60mgkg
-1The tail point is got hematometry mouse blood sugar, blood glucose value＞20.0mmolL behind the 72h
-1Mice be chosen as diabetic mice, be divided into model group (10) (model group and normal control group feedwater), embodiment 1 Radix Bauhiniae Hupehanae Bauhinia hupehara C extract group (500mg/kg, 10), metformin positive drug group (250mg/kg, 10).Gastric infusion is 18 days continuously, the 7th day mensuration blood glucose, the 18th day mensuration blood glucose.Data represent that with X ± SD two groups of mean differences are analyzed with the t check.The result shows that the Radix Bauhiniae Hupehanae extract has obvious blood sugar lowering and the effect of losing weight to the alloxan diabetes mice.
Table 1 Radix Bauhiniae Hupehanae is to the influence of alloxan diabetes mouse blood sugar
Annotate: numerical value=meansigma methods ± S.D., compare * P＜0.05, * * P＜0.01, * * * P＜0.001 with model group.n＝10。
Table 2 Radix Bauhiniae Hupehanae extract is to the influence of alloxan diabetes mice body weight
Annotate: numerical value=meansigma methods ± S.D., compare * P＜0.05, * * P＜0.01 with model group.n＝10
Claims (11)
- Radix Bauhiniae Hupehanae, Radix Bauhiniae Hupehanae extract, the compositions that comprises the compositions of Radix Bauhiniae Hupehanae or comprise the Radix Bauhiniae Hupehanae extract for the preparation of prevention, slow down or treat purposes in the product of diabetes or obesity, wherein said Radix Bauhiniae Hupehanae is selected from the stem of Radix Bauhiniae Hupehanae and the leaf of Radix Bauhiniae Hupehanae; Wherein said Radix Bauhiniae Hupehanae extract is to prepare by the method that may further comprise the steps:Obtain the Radix Bauhiniae Hupehanae vegetable material; Water, organic solvent or water-containing organic solvent extract the Radix Bauhiniae Hupehanae vegetable material, obtain the Radix Bauhiniae Hupehanae extract, the step that wherein said water, organic solvent or water-containing organic solvent extract the Radix Bauhiniae Hupehanae vegetable material comprises: (1) water, organic solvent or water-containing organic solvent under proper condition soaks the Radix Bauhiniae Hupehanae vegetable material; (2) separate liquid phase and solid phase; (3) randomly, repeating step (1) and (2) one or many; (4) randomly, merge liquid phase; (5) randomly, will be concentrated by the liquid phase that step (2) or step (4) obtain, wherein, described organic solvent is methanol, ethanol, propanol, acetone or n-butyl alcohol or their any combination; Described water-containing organic solvent is the aqueous solution that contains organic solvent, and wherein said organic solvent is methanol, ethanol, propanol, acetone or n-butyl alcohol or their any combination; Wherein, the described compositions that comprises Radix Bauhiniae Hupehanae is made up of Radix Bauhiniae Hupehanae and excipient or carrier; The described compositions that comprises the Radix Bauhiniae Hupehanae extract is made up of Radix Bauhiniae Hupehanae extract and excipient or carrier.
- 2. the purposes of claim 1, wherein the number of times that repeats in step (3) is 1-5 time.
- 3. the purposes of claim 1, wherein the number of times that repeats in step (3) is 1-2 time.
- 4. the purposes of claim 1, wherein the number of times that repeats in step (3) is 2-5 time.
- 5. the purposes of claim 1, wherein the number of times that repeats in step (3) is 3 times.
- 6. the purposes of claim 1, wherein the number of times that repeats in step (3) is 4 times.
- 7. the purposes of claim 1, wherein felicity condition comprises described in the step (1): temperature is 0-100 ℃; Pressure is 0.1 atmospheric pressure-5 atmospheric pressure; Soak time is at least greater than 0.5 hour, grows to 5 days; The w/v of Radix Bauhiniae Hupehanae vegetable material and solvent is 1: 1 to 1: 20 kg/liter; When soaking, randomly stir.
- 8. the purposes of claim 7, wherein said pressure is 0.5 atmospheric pressure-3 atmospheric pressure.
- 9. the purposes of claim 7, wherein said pressure is normal pressure.
- 10. the purposes of claim 1, wherein separate in the step (2) by filter, centrifugal or natural subsidence realizes.
- 11. the purposes of claim 1, wherein the concentrated of step (5) is concentrating under reduced pressure, and temperature is at 40-70 ℃; Perhaps concentrating is lyophilization.
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| CN 200510093358 CN1919229B (en) | 2005-08-26 | 2005-08-26 | Bauhinia hupehara C. and its extract for the treatment of diabetes and obesity |
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| CN 200510093358 CN1919229B (en) | 2005-08-26 | 2005-08-26 | Bauhinia hupehara C. and its extract for the treatment of diabetes and obesity |
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| CN1919229A CN1919229A (en) | 2007-02-28 |
| CN1919229B true CN1919229B (en) | 2013-07-03 |
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| US20050129788A1 (en) * | 2002-04-23 | 2005-06-16 | Agreda Navajas Juan C. | Herbal product to be administered to diabetic individuals and the production method thereof |
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