CN106420932A - Composition capable of increasing insulin sensitivity, and applications thereof - Google Patents

Composition capable of increasing insulin sensitivity, and applications thereof Download PDF

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CN106420932A
CN106420932A CN201610793976.8A CN201610793976A CN106420932A CN 106420932 A CN106420932 A CN 106420932A CN 201610793976 A CN201610793976 A CN 201610793976A CN 106420932 A CN106420932 A CN 106420932A
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mori
compositionss
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insulin sensitivity
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CN106420932B (en
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步世忠
罗春
袁燕燕
邹欣蓉
刘冰洋
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Ningbo University
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
    • A61K36/605—Morus (mulberry)
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    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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Abstract

The invention discloses a composition capable of increasing insulin sensitivity. The composition is characterized by being mixed and extracted from the following raw material in percentage by weight: 10-20% of silkworm excrement, 50-55% of cortex mori and 25-40% of folium mori. The composition shows a good effect on increasing the insulin sensitivity.

Description

A kind of compositionss increasing insulin sensitivity and its application
Technical field
The present invention relates to a kind of compositionss increasing insulin sensitivity, said composition is by Bombyx mori L. 10～20%, Cortex Mori 50～55%, Folium Mori 25～40% mixed extraction.
Background technology
Mulberry is perennial Moraceae Mulberry plant, tropical, sub- in Asia, Europe, North America, South America, Africa and India The torrid zone, temperate zone etc. are widely distributed, have plantation the province silkworm cultivation base such as China southeast Zhejiang, Jiangsu, containing abundant Huang more Letones.Cortex Mori extract is derived from the dry root skin of Moraceae Mulberry plant, and winter excavates, and cleans, and takes advantage of fresh scraping off and carries on the back palm fibre Color cork, separates skin zone and core, strips Rhizoma Euonymus, dry.Multinomial research confirms, Cortex Mori extract (mori cortex Extract) there are multiple medicinal efficacies, including antiinflammatory, antihepatocarcinoma effect, such as induce cancerous cell to wither by suppressing microtubules Die, eliminate the pneumonia including bronchitis.Cortex Mori extract passes through the two-way phosphorylation of glucocorticoid receptor (GR) To Hippocampus, there is antidepressant effect.Cortex Mori extract phellinus ketone G is alternatively arranged as the antibacterial of cariogenic bacteria.
The main chemical compositions of Cortex Mori have flavonoid, Coumarinses, polysaccharide and other compound.Flavonoid master Mulberrin to be included, cyclomulberrochromene element, Morusin, cyclomorusin, mulberrochromene, hydroxyl dihydro Morusin, morindone A～V, Cortex Mori root Plain hydroperoxides, Morusin -4- glucose, ring Mulberry tryptophol, flavanone sanggenon compound A, Mulberry glycosides A-D, Radix Mori Skin alcohol, 5,7- dihydroxy chromone, the chalcone A that rubs etc..Coumarin include scopoletin, 5, umbelliferone, scopoletin (6- methoxyl group -7- hydroxyl-coumarin), umbrella shape spend lactone.Polysaccharide includes shitosan, chitin, mucoitin, Mulberry polysaccharide.Its Its compound also include Mulberry Aglycone A, 1-DNJ, stibene glucoside type compound, Morusin (A, B, C, D, F, G), butanol, glycoside derivant, tannin, sitosterol, the acid of birch skin, volatile oil, daucosterol, oleanolic acid, ursolic acid, 3- methoxy Base -4- hydroxy-benzaldehyde etc..
The research report such as Liu Xiaowen, the alcohol extraction liquid energy of Cortex Mori suppresses the sucrase active of enteric epithelium, delays the suction of monosaccharide Receive, thus reducing the rapid rising of post-prandial glycemia；The research such as Hikino is found that Cortex Mori has another composition of hypoglycemic activity The glycoprotein of moran A can substantially reduce the blood sugar level of diabetic mice caused by normal mouse and alloxan induction；Week The high fat diet such as cutting edge of a knife or a sword is equipped with lumbar injection STZ and sets up diabetes rat model, inquires into the blood sugar reducing function of white mulberry rootbark general flavone, knot Fruit finds that white mulberry rootbark general flavone can reduce blood glucose and the triglyceride levels of type 2 diabetes mellitus rat, has anti-diabetic and regulation The effect of blood fat；The diabetes rat that Zhong Guolian etc. is induced with STZ, gastric infusion Cortex Mori aqueous extract studies it to diabetes The impact of rat glycolipid metabolism, finds there is hypoglycemic activity to experimental diabetic rats, but blood fat is no affected；Wang Ning etc. Research finds, Cortex Mori aqueous extract, in the case of not having insulin action, promotes HepG2Hepatocyte disappears to glucose Consumption, the HepG that low dosage insulin is connected to2Cell Sugar intake has the enhanced effect of auxiliary.Horse soughing of the wind in the pines etc. uses Cortex Mori extract The diabetes rat that gavage induces to alloxan, after administration a period of time, result shows the neuropathy to diabetes rat Improve significantly, the area of transverse section around neural myelin can be increased, mitigate edema, and MDA content can be reduced, increase SOD activity, the pathological changes of diabetes-alleviating Peripheral Nerves in Rats early stage；Find, Cortex Mori extract can increase sciatic nerve simultaneously Synapse cellulose content, improves or increases sensation and the motor conduction velocity of rat sciatic nerve, shorten incubation period；In addition, Cortex Mori Extract can improve the activity of sodiumpotassium pump enzyme, increase the content of sciatic nerve cGMP, cAMP, have one to diabetic neuropathy Fixed preventive and therapeutic effect.
Bombyx mori L. is rich in chlorophyll, pectin, flavonoid, crude protein, and the necessary trace element of part human body etc. effectively becomes Point.Research about Bombyx mori L. chemical composition is constantly being goed deep into always《China's book on Chinese herbal medicine》Record：Bombyx mori L. contains phyllins, bag Include pheophytin a and b, 13- hydroxyl pheophytin a and b, 10- hydroxyl pheophytin a etc..《Dictionary of medicinal plant》Conclude： Bombyx mori L. contains Organic substance 83.77～90.44%, ash 9.56～16.23%, total nitrogen 1.91～3.60%, and pohytol 0.25～ 0.29%.Also contain chlorophyll, free amino acid, not saponification composition cupreol, cholesterol, ergosterol and carnaubyl alcohol, Serpentiss Numb lipidol, cupreol-β-glycoside, copper, a large amount of carotene and vitamin B and auxin-miscellaneous are grown up strong and sturdy Element.Yan Xinpei etc. summarizes：Bombyx mori L. contain 70 crude protein, crude fat, crude fibre, nitrogen-free extract, ash, water etc., also contain chlorophyll, Carotenoid, pectin, leaf protein etc.；Triacontanol, vitamin A, B, D, solanesol, Bombyx mori L. ketone, β-paddy is also contained in Bombyx mori L. Steroid-D-Glucose, plumage Seem Lablab Album alcohol, ergosterol, sitosterol, cholesterol, tetracosanol, Lupeol, 13 kinds of free amino acid, The necessary trace element of the human bodies such as auxin, heteroauxin and copper.Wei Kemin etc. reports：Abundant battalion is contained in Bombyx mori L. Foster material, modern study shows, air-dry after silkworm in spring sand in containing 13.6% crude protein, 3.7% fat, 12.5% thick Fiber, 75 nitrogen-free extract 48.7%, ash 12.6%, calcium 3.2%.In Bombyx mori L. in addition to containing abundant chlorophyll, also class The nutrient such as the compositions such as carotene, pectin, leaf protein and phosphorus, potassium, silicon, magnesium.Cui's stannum is strong to be waited from 60% ethanol extraction In Ethyl acetate extract, isolate scopoletin, umbelliferone, dihydrouracil, uracil, blumenol- first A, loliolide, benzoic acid, 9,16-dioxo-10,12,14-octadecatrienoicacid.Period-luminosity hero etc. applies macropore Multiple column chromatography means such as resin, ion exchange resin, polydextran gel isolate and purify alkaloid component, with mass spectrum and nuclear-magnetism etc. Spectrographic technique determines the structure of compound, isolates 1-DNJ, fagomine and 3-epifagomine biology first Alkali composition.Cui's stannum waits by force application chromatographic technique separating and purifying technology, ethyl acetate portion from Bombyx mori L. 95% ethanol extraction first Point, isolate 3S, 5R-dihydroxy-6R, 7-megstigmadien-9-one, 3S, 5R-dihydroxy-6S, 7- Megstigmadien-9-one, (6R, 9R) -3-oxo- α-ionol β-D-glucopyranoside, (6R, 9S) -3-oxo- α - Ionol- β-D-glucopyranoside, blumenol C glucoside, byzantionoside B, alangionoside A series of sesquiterpene such as L and its glycosides compound；Also separately obtain compound phylloxanthin in fatty contents；Divide from water section From obtain water soluble ingredient compound pipecoline acid, glycine betaine, alanine, glutamic acid, Phenylalanine, leucine, isoleucine.
Since ancient times, the traditional Chinese medical science just using Folium Mori as treatment diabetess (i.e. the diabetes of modern medicine) application in TCM in facing Bed, Japanese ancient book《Eat tea health preserving note》Also recording Folium Mori plays the role of improvement " drinking-water disease " (i.e. the diabetes of modern medicine).State Inside and outside research data confirms, alkaloid and polysaccharide are main blood sugar reducing components in Folium Mori.
The hypoglycemic activity of Folium Mori is to be realized by two approach：One is that (buttocks is mould for 1- deoxidation open country by alkaloid DNJ Element) enzymatic activity generation inhibitory action is decomposed to disaccharides, thus suppressing the absorption to disaccharidase for the small intestinal, reduce the peak of blood glucose after food Value；Two be by Folium Mori alkaloid fagomine and polysaccharides of Folium Mori promote β cell divide must insulin, and insulin can promote carefully Born of the same parents to sugared utilization, hepatic glycogen synthesis and improve carbohydrate metabolism, are finally reached hypoglycemic effect.
In sum, Bombyx mori L., Cortex Mori and Folium Mori all have the hypoglycemic effect of fall, but because their composition is multiple Miscellaneous, specifically which kind of composition blood sugar reducing function is played by what kind of mechanism by and do not know.Then in treatment diabetes mellitus In, hypoglycemia is typically to treat the complication of diabetes, and hypoglycemia is harmful to human body, especially to elderly patients, low blood The harm of sugar is more more than hyperglycemia.Hypoglycemic harm mainly has：1st, during hypoglycemia, internal epinephrine, glucocorticoid, Glucagon and growth hormone etc. rise C-21 cortico-steroid to be increased, and leads to react sexual intercourse blood glucose (Lignum Sappan outstanding person's effect), causes blood glucose fluctuation, Aggravation；2nd, prolonged and repeated serious hypoglycemic episodes may result in the irreversible infringement of central nervous system, causes patient's property Lattice make a variation, Mental Subnormality, dementia etc.；3rd, hypoglycemia can also stimulate cardiovascular system, inspires arrhythmia, myocardial infarction, brain Apoplexy etc.；4th, hypoglycemic coma is undiscovered too long causes death.
In addition, different extracting method, the substance classes extracted are had very big from Bombyx mori L., Cortex Mori and Folium Mori Difference has the extract showing Bombyx mori L., Cortex Mori and Folium Mori to have a certain degree of function of reducing blood sugar although different reports, But mixed extract and extracting method all do not have been reported that for three.
Content of the invention
The present inventor through creatively test and makes great efforts it was found that a kind of new extract is for increase by persistence The sensitivity of insulin and reduction blood glucose have significant effect.It is an object of the invention to provide a kind of new increase insulin is quick The compositionss of perception, described compositionss are to be extracted by after Bombyx mori L. 10～20%, Cortex Mori 50～55%, Folium Mori 25～40% mixing Gained.
The Bombyx mori L. of the present invention refers to silkworm faeces, the Folium Mori that are mixed into, casts off a skin or other debris are separated when removing sand.The present invention Cortex Mori be the dry root skin of moraceae plants mulberry Morus alba L. or the dried object of mulberry root.The present invention's Folium Mori, also from the leaveves of moraceae plants mulberry Morus alba L., dry standby after harvesting.
Above-mentioned raw materials from farmers''s buying or can be provided for oneself, after above-mentioned raw materials Bombyx mori L., Cortex Mori, Folium Mori are dried, is ground into dry Dry powder.The mixed proportion of above-mentioned dried powder is Bombyx mori L. 10～20%, Cortex Mori 50～55%, Folium Mori 25～40%.
A kind of extracting method of the compositionss increasing insulin sensitivity, comprises the following steps：
Pulverising step, after Bombyx mori L., Cortex Mori, Folium Mori are separately dried, is ground into dried powder；
Blend step, by the dried powder stirring of above-mentioned Bombyx mori L. 10～20%, Cortex Mori 50～55%, Folium Mori 25～40% Mix, obtain mixture；
Leach step, said mixture is mixed with extracting solution, and described extraction solution is the 1 of water content 40～60%, 3- butanediol, mixed liquor is placed in stirred in water bath and extracts, described bath temperature is 80～95 DEG C；
Filtration step, the mixed liquor after extraction is filtrated to get filtrate, and using NaOH, pH value is adjusted to 10；
Concentration step, the 20～50% of above-mentioned filtrate reduced in volume to original content；
Ion exchange resin treatment step, above-mentioned concentrated solution is passed through film process or ion exchange resin, obtains compositionss Stock solution；
Drying steps, above-mentioned composition mixed liquor are dried, obtain brown powder.
Brief description
The comparison of T-CHOL TC in Fig. 1 each group rat blood serum.
The comparison of triglyceride TG in Fig. 2 each group rat blood serum.
The blood glucose situation when 0,30,60,120min for Fig. 3 OGTT experiment.
The statistical result of area AUC under Fig. 4 OGTT empirical curve.
Specific embodiment
Embodiment 1
The Bombyx mori L. of the present invention, Cortex Mori, Folium Mori come from the silkworm raiser of Zhejiang Province northern territory, by the silkworm of purchase at peasant household Sand, Cortex Mori, Folium Mori deionized water are cleaned, and are placed in 30 DEG C of drying baker overnight, after obtaining dry product, with mortar or pulverizing Machine grinds to powder or molecule.Then weigh Bombyx mori L. 200g, Cortex Mori 500g, Folium Mori 300g mix homogeneously respectively, add To in the big flask extracting solution equipped with 5L, described extraction solution is the 1,3 butylene glycol of water content 50%.
Bath temperature is set to 85 DEG C, stirring and leaching 48 hours, then use filter paper filtering (whatman company), obtain filter Liquid 3.72L.Use 1M NaOH solution, filtrate pH value is adjusted to 10.Concentrate filtrate to using under Rotary Evaporators, room temperature 800ml.HPD400A macroporous resin column on the concentrated solution obtaining is adsorbed, the loading of resin column is 5Kg, and blade diameter length ratio is 1：4, Loading 3 times repeatedly, staticaccelerator adsorption 60 minutes.
First with the deionized water drip washing resin column of 4 times of column volumes；Again with 20% alkaline ethanol (NaOH, pH of 8 times of column volumes =10) drip washing；Then 20% ethanol rinse with 3 times of column volumes is extremely neutral；To be adsorbed on resin with 6 times of column volume 60% ethanol Extract eluting.
Eluting also vacuum distillation is reclaimed ethanol, is then vacuum dried, and dried solid was pulverized 100 mesh Sieve, obtains 102.1g extract, is denoted as compositionss A.
Embodiment 2
According to the method for embodiment 1, extracted using the Bombyx mori L. of 1,000g, Cortex Mori, Folium Mori respectively, finally obtained respectively To 78.4g compositionss B, 104.3g compositionss C and 112.8g compositionss D.
Embodiment 3 compositionss increase the effect measuring of insulin sensitivity
1.II patients with type Ⅰ DM modelling
The rat limosis 12h that high lipid food is fed, with the dosage disposable celiac injection 1% chain urea assistant bacterium of 30mg/kg Element (the 0.1mol/L sodium citrate buffer solution dissolving being 4.2～4.4 with pH, using front preparation), standard diet group is synchronized with abdomen Isodose sodium citrate buffer solution is injected in chamber.After 72h STZ injection rat cut tail point venous blood sampling fasting glucose (FBG, Mmol/L) it is estimated, a touch blood glucose meter surveys FBG, with blood glucose value >=13.8mmol/L for the successful standard of rat molding.
2. composition treatment
Treatment stage implements the present composition A, B, C, D treatment to treating diabetes group within 12nd～20 week.Extract Given low is 10g/kg d.For carrying out comparison, remaining rat is also adopted by gavage mode and gives equal-volume normal saline.
3. blood sugar detection
Each group animal surveys FBG once in synchronization weekly during testing, and before surveying FBG, Rat Fast be can't help water 12h, Next day rat-tail point venous blood sampling, measures blood sugar concentration with a touch blood glucose meter and carries out relative recording.
4. oral glucose tolerance test
Carry out oral glucose tolerance test (OGTT) at the end for the treatment of stage.Empty stomach 12h rat tail point venous blood collection is surveyed empty Abdomen blood glucose value is designated as the blood glucose value of 0h.Carry out gavage by 2.0g/kg dosage with 50% sugar juice afterwards, respectively at 30min, 60min, 120min take tail point venous blood to survey blood glucose.5 group rat OGTT curves are drawn in experiment after terminating, abscissa is the time (min), vertical coordinate is blood sugar concentration (mmol/L).And under calculated curve area (area under curve, AUC) judging sugar Tolerance state.
AUC (h.mmol/L)=1/4 × 0h+1/2 × 0.5h+3/4 × 1h+2h.(formula 1)
Experimentation
30 male SD rats are drunk water in the free diet of Animal House to shake down, 1 week by a definite date.It is randomly divided into N (normal Group), M (diabetic groups), M+A (treating diabetes group, put into the present invention extract A), M+B (treating diabetes group, put into this Invention extract B), M+C (treating diabetes group, put into the present invention extract C), M+D (treating diabetes group, put into this The extract D of invention) 6 experimental grouies, every group 5.N group whole process normal diet is raised, and M (diabetic groups), M+A (control by diabetes Treatment group), M+B (treating diabetes group), M+C (treating diabetes group), M+D (treating diabetes group) high the fat stage continues 12 weeks, Then lumbar injection 1%STZ (30mg/kg), injects the citric acid of equivalent in the same fashion for eliminating variable impact to N group rat Buffer.After 72h, the rat of STZ injection is cut tail point venous blood sampling fasting glucose (FBG, mmol/L) and is estimated, a touch Blood glucose meter surveys FBG, with blood glucose value >=13.8mmol/L for the successful standard of rat molding.After molding success, treatment group enters treatment In the stage, carry out gavage using the aqueous solution (10g/kg d) of extract A, B, C, D compositionss respectively, and gastric infusion continues 8 Week.Carry out OGTT test at the end of experiment in 20th week；Then put to death rat, blood plasma is carried out with the biochemistry detection of correlation, pathology divides The configuration situation of analysis liver, Western blot method detects fatty acid synthesis related gene.
Experimental result
1. extract A has more preferable effect for blood fat reducing TC (T-CHOL), TG (triglyceride) level
As depicted in figs. 1 and 2, with diabetes rat ratio, extract A of the present invention, TC, TG of B, C, D treatment group, under all having Fall, but extract A treatment group of the present invention is remarkably decreased (P<0.05), almost normal mouse level, therefore, with extract B, C, D compare, and the extract A of the present invention has more preferable effect for reduction blood fat.
2. extract A shows more preferable effect for increasing insulin sensitivity
In order to assess the carbohydrate tolerance situation after extract drug treatment of the present invention of rat, carry out at the end of experiment OGTT tests, such as Fig. 3, the blood glucose situation when 0,30,60,120min for the OGTT experiment.Diabetes rats AUC are just significantly higher than Often rat (P<0.05), as shown in figure 4, comparing with diabetic groups, extract A of the present invention, B, C, D treatment group AUC all notable Lower, and compared with extract B, C, D, the extract A of the present invention has more preferable effect for increasing insulin sensitivity.

Claims (6)

1. a kind of compositionss increasing insulin sensitivity are it is characterised in that described compositionss are by Bombyx mori L. 10～20%, Cortex Mori 50～55%, Folium Mori 25～40% mixed extraction.
2. application in preparation treatment/prevention diabetes medicament for the compositionss as claimed in claim 1.
3. a kind of extracting method of the compositionss increasing insulin sensitivity is it is characterised in that comprise the following steps：
Pulverising step, after Bombyx mori L., Cortex Mori, Folium Mori are separately dried, is ground into dried powder；
Blend step, the dried powder of the Bombyx mori L. 10～20% after pulverizing, Cortex Mori 50～55%, Folium Mori 25～40% is stirred Mix, obtain mixture；
Leach step, described mixture is mixed with extracting solution, and described extraction solution is 1, the 3- fourth of water content 40～60% Glycol, obtains mixed liquor, described mixed liquor is placed in stirred in water bath and extracts, described bath temperature is 80～95 DEG C；
Filtration step, the mixed liquor after extraction is filtrated to get filtrate, and using NaOH, the pH value of described filtrate is adjusted to 10；
Concentration step, by the 20～50% of described filtrate reduced in volume to original content；
Ion exchange resin treatment step, above-mentioned concentrated solution is passed through ion exchange resin treatment, obtains compositionss stock solution；
Drying steps, above-mentioned composition stock solution lyophilization obtains brown powder.
4. method as claimed in claim 3, described extraction solution is the 1,3 butylene glycol of water content 50%.
5. method as claimed in claim 3, described bath temperature is 85 DEG C.
6. method as claimed in claim 3, in described leach step, extraction time is not less than 24 hours.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110279719A (en) * 2019-06-27 2019-09-27 北京市房山区中医医院 It treats and presses packet and its preparation and application outside the late silkworm excrement of diabetic gastroparesis

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1559539A (en) * 2004-02-23 2005-01-05 南开大学 Medicinal mixture possessing alpha glycocidase inhibiting activity and its use
CN101129476A (en) * 2006-08-21 2008-02-27 中国医学科学院药物研究所 Application of mulberry twigs alkaloid valid target in preparing antidiabetics medicament
CN101336964A (en) * 2008-08-19 2009-01-07 苏州大学 Mulberry bark extract capable of reducing blood sugar and preparation method thereof
CN101926853A (en) * 2009-06-25 2010-12-29 浙江京新药业股份有限公司 Medicine composition with function of lowering blood sugar level as well as preparation method and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1559539A (en) * 2004-02-23 2005-01-05 南开大学 Medicinal mixture possessing alpha glycocidase inhibiting activity and its use
CN101129476A (en) * 2006-08-21 2008-02-27 中国医学科学院药物研究所 Application of mulberry twigs alkaloid valid target in preparing antidiabetics medicament
CN101336964A (en) * 2008-08-19 2009-01-07 苏州大学 Mulberry bark extract capable of reducing blood sugar and preparation method thereof
CN101926853A (en) * 2009-06-25 2010-12-29 浙江京新药业股份有限公司 Medicine composition with function of lowering blood sugar level as well as preparation method and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110279719A (en) * 2019-06-27 2019-09-27 北京市房山区中医医院 It treats and presses packet and its preparation and application outside the late silkworm excrement of diabetic gastroparesis

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