CN110024611A - A kind of betel nut alkali process improves the method and application of betel nut disease resistance - Google Patents
A kind of betel nut alkali process improves the method and application of betel nut disease resistance Download PDFInfo
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- CN110024611A CN110024611A CN201910437732.XA CN201910437732A CN110024611A CN 110024611 A CN110024611 A CN 110024611A CN 201910437732 A CN201910437732 A CN 201910437732A CN 110024611 A CN110024611 A CN 110024611A
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- 244000080767 Areca catechu Species 0.000 title claims abstract description 89
- 235000006226 Areca catechu Nutrition 0.000 title claims abstract description 89
- 208000035240 Disease Resistance Diseases 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 36
- 239000003513 alkali Substances 0.000 title description 5
- 230000008569 process Effects 0.000 title description 4
- HJJPJSXJAXAIPN-UHFFFAOYSA-N arecoline Chemical compound COC(=O)C1=CCCN(C)C1 HJJPJSXJAXAIPN-UHFFFAOYSA-N 0.000 claims abstract description 44
- 238000012545 processing Methods 0.000 claims abstract description 30
- 230000006698 induction Effects 0.000 claims abstract description 22
- 238000005507 spraying Methods 0.000 claims abstract description 8
- 230000003698 anagen phase Effects 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 235000013399 edible fruits Nutrition 0.000 claims description 16
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 15
- 201000010099 disease Diseases 0.000 claims description 14
- 239000002689 soil Substances 0.000 claims description 12
- 241000196324 Embryophyta Species 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000003337 fertilizer Substances 0.000 claims description 6
- 239000004576 sand Substances 0.000 claims description 6
- 241000193738 Bacillus anthracis Species 0.000 claims description 5
- 235000016709 nutrition Nutrition 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 244000052769 pathogen Species 0.000 claims description 4
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- 238000009423 ventilation Methods 0.000 claims description 3
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 2
- 230000035784 germination Effects 0.000 claims description 2
- 230000005070 ripening Effects 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 6
- 230000018109 developmental process Effects 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 3
- 229940079593 drug Drugs 0.000 abstract 1
- 235000008216 herbs Nutrition 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 description 10
- 230000003902 lesion Effects 0.000 description 9
- 241001480650 Colletotrichum kahawae Species 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
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- 241001133184 Colletotrichum agaves Species 0.000 description 2
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229930013930 alkaloid Natural products 0.000 description 2
- 150000003797 alkaloid derivatives Chemical class 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
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- 239000003292 glue Substances 0.000 description 2
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- 241001123536 Colletotrichum acutatum Species 0.000 description 1
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- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
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- 239000003864 humus Substances 0.000 description 1
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- 210000003079 salivary gland Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- COEZWFYORILMOM-UHFFFAOYSA-N sodium 4-[(2,4-dihydroxyphenyl)diazenyl]benzenesulfonic acid Chemical compound [Na+].OC1=CC(O)=CC=C1N=NC1=CC=C(S(O)(=O)=O)C=C1 COEZWFYORILMOM-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of methods that arecaline induces processing to improve betel nut disease resistance, this method includes that plantation nursery, seedling management and arecaline induce processing step, the arecaline induction processing step includes: to the betel nut seedling for being in growth phase any period, the betel nut aqueous slkali for being continuously 200 μM -1000 μM to leaf surface spraying concentration, time are 5-14 days.The method of the present invention is simple and easy, easy to operate, takes effect quick, the disease resistance of betel nut can be improved in a short time, while the drug is medicine based on herbs, is of great significance to maintenance betel nut industry sustainable and healthy development.
Description
Technical field
The invention belongs to the disease-resistant technical fields of betel nut, are improved in particular to one kind by short time betel nut alkali process
The method of betel nut disease resistance.
Background technique
Betel nut (ArecacatechuL.) belongs to Palmae Areca perennial evergreen magaphanerophytes, and stem is upright, arbor shape, high
More than 10 meters, 30 meters are reached as high as, there is an apparent annular leaf trace, monoecism, inflorescence multi-branched, ovary oblong, fruit is oval
Shape or ovoid, seed is oval, the flowering fruit bearing stage 3-4 month, is the main industrial crops in China torrid areas, main product in Hainan Province and
Taiwan.Betel nut is one of four great Nan medicine of China (betel nut, fructus amomi, intelligence development, Morinda offcinalis How), and medicinal range is wide, and economic value is high.Bin
Bulky original Malaysia is mainly distributed on Southeast Asia, Tropical Asian area, East Africa and Some European region, Pacific region master
It is distributed in Papua New Guinea, Solomon Islands, Fiji, Vanuatu and Micronesia, is sporadicly distributed in skin
The island Na Pei, beauty belong to Mariana Islands and the Marshall Islands, and betel nut plantation in Hawaiian Islands is used only as watching.Hainan be China most
The big betel nut place of production, 99% (not counting Taiwan) of Zhan Quanguo yield；Gaozhou county and Leizhou of Guangdong Province east are also distributed, but
Upgrowth situation is bad；Also there are a small amount of plantation in Guangxi, Fujian and Yunnan some areas as ornamental trees.
The warm and humid heat type sun plant of Areca, the climatic environment pyrophilous, abundant rainfall is wet.It is common scattered in low mountain valley
Bottom, ridge foot, footslope and the secondary woodland of Plain small stream side tropical monsoon forest also have and are grown on rich humus-containing cheuch in flakes, mountain bank,
In sparse woods and subacidity is to neutral sandy loam deserted mountain wilderness.It is mainly distributed between 28 ° of north and south latitude, most suitable temperature is in 10-
36 DEG C, minimum temperature is not higher than 40 DEG C not less than 10 DEG C, maximum temperature, be advantageous to the generations of all kinds of disease pest and weeds with for
Evil.It is reported that external betel nut disease include anthracnose, yellows, leaf spot, leaf bacterial stripe, dry rot, yellowtop,
Root rot, leaf blight, epidemic disease, fruit ear wilt disease, bud rot, algae pinta, dark mildew etc., wherein Major Diseases have anthracnose, leaf
Pinta, bacterial stripe, dry rot, root rot, and Hainan Province's betel nut Major Diseases are yellows, anthracnose, bacillary item
Pinta, algae pinta, dark mildew, leaf spot and root rot etc..
Plant anthracnose is often concurrently caused by a variety of pathogens, as rubber, mango anthracnose by glue born of the same parents' anthrax-bacilus
(C.gloeosporioides) cause with sharp spore anthrax-bacilus (C.acutatum).The anthracnose that betel nut is previously reported can also be by glue
Born of the same parents' anthrax-bacilus (C.gloeosporioides) causes.Disease survey and identification discovery, coffee anthrax-bacilus are carried out for the previous period
(C.kahawae) betel nut anthracnose can also be caused.Anthrax pathogen adaptation viability is stronger, in the main betel nut growing area in China
There is generation, and the situation very severe that causes harm.In production mainly by the methods of agricultural measures and medical treatment to the disease into
Row prevention and treatment, still shortage effectively preventing method.Therefore, how its disease resistance is improved in a short time, produced with reducing disease to betel nut
The influence of industry steadily improves the economic benefit of betel nut industry, it has also become betel nut industry sustainable and healthy development reality urgently to be resolved
Problem.
Arecaline belongs to one kind of alkaloid, because it is primarily present in betel nut, therefore is named as arecaline, is betel nut
The alkaloid that content is most in material composition is learned, content reaches 0.3%-0.63%, is that primary healthcare and the pharmacology of betel nut are living
One of property ingredient.Arecaline is highly soluble in water, is soluble in methanol, is dissolved in ethyl alcohol.Arecaline can stimulate m receptor to compensate acetyl gallbladder
The deficiency of alkali promotes body excited, improves learning and memory ability；Arecaline has under doses increases salivary gland secretion
Effect is conducive to digestion, and stomach is promoted to move normalization；Arecaline also has antiatherosclerosis and antithrombotic work
With；In addition, arecaline is to Escherichia coli, staphylococcus aureus, bacillus subtilis, Bacillus cercus, Salmonella gallinarum
Etc. Gram-negatives and gram-positive bacterium have inhibiting effect, and the research to the inhibiting effect of fungal microbe there is not yet
Report.
How betel nut disease resistance is further increased, and be colonized betel nut can preferably in relevant environment stress is mesh
One hot spot of preceding research.Currently, not yet quickly improve betel nut disease resistance method, therefore using allogenic material at
Reason maintains betel nut industry sustainable and healthy development to be of great significance to improve betel nut disease resistance.
Summary of the invention
In view of the deficiencies in the prior art, the purpose of the invention is to provide a kind of methods for improving disease resistance of plant, especially
Its method for inducing processing to improve betel nut disease resistance by arecaline.This method is simple and easy, easy to operate, takes effect quick,
Betel nut disease resistance can be improved, in a short time to maintain betel nut industry sustainable and healthy development to be of great significance.
In order to realize above-mentioned goal of the invention, the present inventor creatively utilize allogenic material arecaline to betel nut seedling into
Row induction processing, to obtain a kind of short time interior method for improving betel nut disease resistance.Specifically, present invention employs following
Technical solution:
The method that a kind of induction processing of arecaline improves betel nut disease resistance, this method include plantation nursery, seedling management and
Arecaline induces processing step, and the arecaline induction processing step includes: to the betel nut for being in growth phase any period
Seedling, the betel nut aqueous slkali for being continuously 200 μM -1000 μM to leaf surface spraying concentration, time are 5-14 days.
It is further preferred that the method that arecaline induction processing improves betel nut disease resistance as described above, wherein starting to leaf
Face sprays the high 20-30cm of betel nut seedling when betel nut aqueous slkali.
It is further preferred that the method that arecaline induction processing improves betel nut disease resistance as described above, wherein continuous spraying
The time of betel nut aqueous slkali is 6-8 days, 2 times/day.
Still further preferably, the method that arecaline induction processing as described above improves betel nut disease resistance, wherein described
Planting nursery step includes:
(1) choose seeds: selection growth 15-30, as a result normal no disease and pests harm, per plant are no less than 300, and hypocarp is many
In 3 supports, 8, blade or more, internode shorter plant in stem top selects second, third support, and fruit shape is oval, oval gold
Yellow ripening fruits is as seed；
(2) vernalization: fruit being placed under sunlight and shines 1-2d, and then in the indoor carry out vernalization of ventilation, ground spreads 1 layer of 10cm
Fine sand, water drenching irrigate, and accumulate fruit, highly between 15-20cm, cover 4-6cm thickness straw, 2-3d drenches 1 water, sorts out germination
Seed is used for nursery；
(3) nursery: selecting bore 12cm, high 15cm, and the black pot for growing seedlings that 4 apertures are leaked in bottom carries out nutritional bag, adds
Enter Nutrition Soil and compound fertilizer, account for bag 4/5, be reloaded into river sand to purseful, every bag puts 1 germinated seeds, and seed bud point is upward, leaching
It is permeable, keep wet, nursery room temperature is controlled at 29-30 DEG C, shady and cool.
It is further preferred that the method that arecaline induction processing improves betel nut disease resistance as described above, wherein the battalion
Soil is supported to mix in surface soil, red soil, decomposed cow dung 1:1:1 ratio.
It is further preferred that the method that arecaline induction processing improves betel nut disease resistance as described above, wherein nursery is in March
First early spring carries out to during late spring by the end of April.
Still further preferably, the method that arecaline induction processing as described above improves betel nut disease resistance, wherein described
Seedling management step includes: 30 days before nursery, and water 300-700L per acre, and ground is kept not do；It 30-90 days, waters per acre
200-500L, and primary grass is removed within one month, 25-40 kilograms of compound fertilizer was applied per acre every 15 days.
Test of the invention is shown, passes through 200 μM of -1000 μ of short time (7 days) continuous spraying various concentration betel nut aqueous slkali
M (molal weight concentration, i.e. every liter of water contain 31.038-155.19mg arecaline) processing, finds processed betel nut material ratio
There is no processed betel nut material to have more disease resistance.It is embodied in, is inoculated with coffee anthrax-bacilus (C.kahawae) after 10 days, system
Count lesion diameter discovery: the average lesion diameter of blank control group is up to 1.3cm or so, and with 200 μM of low concentration betel nut aqueous slkali-
Lesion diameter is respectively after 1000 μM (molal weight concentration, i.e. every liter of water contain 31.038-155.19mg arecaline) processing
1.1cm and 0.9cm or so, and 1000 μM of betel nut aqueous slkalis treated lesion diameter is 4/5 or so of blank control group, variance
Analysis shows that the difference with statistical significance.Based on the studies above achievement, the present invention also provides arecalines to mention in a short time
Application in high betel nut disease resistance, the disease resistance are the performances of anti-anthracnose.It is further preferred that the anthracnose is
Anthracnose caused by coffee anthrax pathogen.
Compared with prior art, the method that arecaline induction processing of the present invention improves betel nut disease resistance has following
Advantage and effect:
(1) arecaline is to inhibit coffee using the natural materials that Bing Lang contains itself as specific to tropical crops betel nut
On the one hand coffee anthrax growth of pathogenic bacteria not will cause pesticide or compound residual, on the other hand will not change contained by betel nut itself
By forming for natural materials, it is of great significance.
(2) the method for the present invention is simple and easy, easy to operate, takes effect significant, the disease resistance of betel nut can be improved in a short time,
To maintain betel nut industry sustainable and healthy development to be of great significance.
Detailed description of the invention
Fig. 1 be after continuously pouring the induction of various concentration betel nut aqueous slkali processing 7 days betel nut to the resistance shadow of betel nut anthrax bacteria
Ring schematic diagram.
Specific embodiment
Technical solution in order to enable those skilled in the art to better understand the present invention, in the following with reference to the drawings and specific embodiments
It further describes, advantages and features of the present invention will be more clear with the description.It should be understood that the embodiment is only
It is exemplary, any restrictions are not constituted to protection scope of the present invention.It will be understood by those skilled in the art that without departing from
Under spirit of the invention can details to technical solution and form modify or replace, but these modifications or substitutions are each fallen within
Protection scope of the present invention.
Embodiment 1: influence of the arecaline induction processing to betel nut anti-subcutaneous ulcer disease disease is tested
1, planting:
(1) choose seeds: betel nut elite stand should select 15-30 raw, and as a result normal no disease and pests harm, per plant are no less than 300,
Hypocarp is no less than 3 supports, and 8, blade or more, the shorter plant of stem top internode.The fruit for selecting second, third to hold in the palm makees kind.
It is best that mellow fruit, which is in golden yellow preferably oval, oval, when fruit is ripe,.
(2) it vernalization: after picking fruit, is placed under sunlight and shines 1-2d, then carry out vernalization.In the interior of ventilation, ground paving 1
Layer 10cm fine sand, water drenching irrigate, and accumulate fruit, highly between 15-20cm, cover 5cm thickness straw, 2-3d drenches 1 water.
(3) nursery: selecting bore 12cm, high 15cm, and the black pot for growing seedlings that 4 apertures are leaked in bottom carries out nutritional bag.Battalion
It supports soil to mix in surface soil, red soil, decomposed cow dung 1:1:1 ratio, compound fertilizer is added, Nutrition Soil accounts for bag 4/5, is reloaded into river sand extremely
Purseful, every bag puts 1 germinated seeds, and seed bud point is upward, water of drenching, and keeps wet, and nursery room temperature is controlled at 30 DEG C, yin
It is cool.It is planted in early spring (at the beginning of 3 months) and late spring (by the end of April) carries out.
2, seedling management:
Nursery initial stage, watering, every 667m2Water 300-600L, and ground is kept not do；30 days before nursery, every 667m2Watering
300-600L；30-90 days, every 667m2Water 200-500L.And primary grass is removed within one month, every 15 days every 667m2Apply 25-40 public affairs
Jin compound fertilizer.
3, arecaline induction is handled:
Normal growth 90 days betel nut seedling, height of seedling 20-30cm, are divided into three parts, first part at the growing way consistent 3 leaf phase
Spray clear water 7 days, second part to spray 200 μM of arecalines (molal weight concentration, i.e. every liter of water contain 31.038mg arecaline) molten
Liquid 7 days, third part sprayed 1000 μM of arecalines (molal weight concentration, i.e. every liter of water contain 155.19mg arecaline) solution 7 days.
Each group sprays 2 times/day, next day after spraying, and stabs inoculation coffee anthrax-bacilus (C.kahawae), observation system to three parts of materials
Count lesion diameter.
By 200 μM -1000 μM of the above short time (7 days) continuous spraying various concentration betel nut aqueous slkali, (molal weight is dense
Degree, i.e., every liter of water contains 31.038-155.19mg arecaline) processing, it is found that processed betel nut material ratio is not processed
Betel nut material is more disease-resistant.As shown in Figure 1, inoculation coffee anthrax-bacilus (C.kahawae), after 10 days, statistics lesion diameter is found: right
According to group average lesion diameter up to 1.3cm or so, and with 200 μM -1000 μM of low concentration betel nut aqueous slkali (molal weight concentration,
I.e. every liter of water contains 31.038-155.19mg arecaline) lesion diameter is respectively 1.1cm and 0.9cm or so after processing, and 1000
Treated that lesion diameter is control group for μM betel nut aqueous slkali (molal weight concentration, i.e. every liter of water contain 155.19mg arecaline)
4/5 or so, variance analysis reaches significant difference.Show that betel nut alkali process can improve betel nut to the resistance of coffee anthrax-bacilus.
Claims (10)
1. a kind of method that arecaline induction processing improves betel nut disease resistance, which is characterized in that this method includes plantation nursery, seedling
Period management and arecaline induce processing step, the arecaline induction processing step include: to it is any in growth phase when
The betel nut seedling of phase, the betel nut aqueous slkali for being continuously 200 μM -1000 μM to leaf surface spraying concentration, time are 5-14 days.
2. the method that arecaline induction processing improves betel nut disease resistance according to claim 1, which is characterized in that start to leaf
Face sprays the high 20-30cm of betel nut seedling when betel nut aqueous slkali.
3. the method that arecaline induction processing improves betel nut disease resistance according to claim 1, which is characterized in that continuous spraying
The time of betel nut aqueous slkali is 6-8 days, 2 times/day.
4. the method that any one of -3 arecaline induction processing improve betel nut disease resistance according to claim 1, which is characterized in that
The plantation nursery step includes:
(1) choose seeds: selection growth 15-30, as a result normal no disease and pests harm, per plant are no less than 300, and hypocarp is no less than 3
Support, 8, blade or more, internode shorter plant in stem top selects second, third support, and fruit shape is oval, oval golden yellow
Ripening fruits is as seed；
(2) vernalization: fruit being placed under sunlight and shines 1-2d, and then in the indoor carry out vernalization of ventilation, it is thin that ground spreads 1 layer of 10cm
Sand, water drenching irrigate, and accumulate fruit, highly between 15-20cm, cover 4-6cm thickness straw, and 2-3d drenches 1 water, sorts out germination kind
Son is used for nursery；
(3) nursery: selecting bore 12cm, high 15cm, and the black pot for growing seedlings that 4 apertures are leaked in bottom carries out nutritional bag, battalion is added
Soil and compound fertilizer are supported, bag 4/5 is accounted for, is reloaded into river sand to purseful, every bag puts 1 germinated seeds, and seed bud point is upward, drenches
Water keeps wet, and nursery room temperature is controlled at 29-30 DEG C, shady and cool.
5. the method that arecaline induction processing improves betel nut disease resistance according to claim 4, which is characterized in that the battalion
Soil is supported to mix in surface soil, red soil, decomposed cow dung 1:1:1 ratio.
6. the method that arecaline induction processing improves betel nut disease resistance according to claim 4, which is characterized in that nursery is 3
The early spring at the beginning of the month carries out to during late spring by the end of April.
7. the method that any one of -3 arecaline induction processing improve betel nut disease resistance according to claim 1, which is characterized in that
The seedling management step includes: 30 days before nursery, and water 300-700L per acre, and ground is kept not do；30-90 days, per acre
Water 200-500L, and removes within one month primary grass, applied 25-40 kilograms of compound fertilizer per acre every 15 days.
8. arecaline improves the application in betel nut disease resistance in a short time, the disease resistance is the performance of anti-anthracnose.
9. arecaline improves the application in betel nut disease resistance in a short time according to claim 8, which is characterized in that described
Short-term is 5-14 days.
10. arecaline improves the application in betel nut disease resistance in a short time according to claim 8, which is characterized in that described
Anthracnose be anthracnose caused by coffee anthrax pathogen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910437732.XA CN110024611B (en) | 2019-05-24 | 2019-05-24 | Method for improving disease resistance of betel nuts through arecoline treatment and application |
Applications Claiming Priority (1)
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