CN104823623B - A kind of method for the weight and artemislnin content for improving sweet wormwood flower - Google Patents
A kind of method for the weight and artemislnin content for improving sweet wormwood flower Download PDFInfo
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Abstract
The present invention relates to a kind of method for the weight and artemislnin content for improving sweet wormwood flower.It is by the several plant auxin such as zinc sulfite, tyrosine, farnesyl diphosphonic acid, mevalonic acid, sodium diethyldithiocarbamate, dimethyl sulfoxide (DMSO), 6 BA, gibberellin, β-heteroauxin, Vc, ethephon (CEPHA),2-(chloroethyl) phosphonic acid, cycocel, qinghaosu biosynthesis intermediate metabolites, secondary substance regulation and control substance and composition of nutritive substance.Its mechanism of action is to promote nutriment to be transported to bud, and bud expands weightening, is circulated while adjusting secondary substance to qinghaosu biosynthesis, increases bud artemislnin content.Promote photosynthate to be flowed to bud, make bud stalwartness volume increase, improve the speed of artemisinin synthesis circulation.Cost is low, is easily received by medicinal herb grower, remarkable in economical benefits.Compared with the control, bud artemislnin content increase by 20% 40%, bud weight increase by 30% 42%.
Description
Technical field
The present invention relates to a kind of method for the weight and artemislnin content for improving sweet wormwood flower.Chemical regulator is taken,
Bud is allowed to expand weightening, while improving its artemislnin content.
Background technology
Sweet wormwood (Artemisiae annie L.), composite family, artemisia.Scientific name artemisia annua, the smelly wormwood artemisia of alias, abrotanum, Artemisia santonica.It is raw
It is longer than hillside, border and wasteland, is annual herb.Common people are used for the treatment extractions relieved summer heat, have a headache, bring down a fever and catch a cold etc.
Qinghaosu is then to utilize sweet wormwood aerial part blade and unopened bud, extracts effective physiologically active ingredient such as sweet wormwood of production
The products such as element are low toxicities, efficient, quick-acting new antimalarial agent (Chinese Pharmacopoeia, 2005；Zhong Guoyue, etc. 2007).Influence sweet wormwood blue or green
The factor of artemisin content has kind, edaphic condition, the sowing time, processing method, harvest time, planting density, illumination condition, applied
Rich water is flat and different parts of sweet wormwood plant etc..Artemisinin synthesis is bud, sweet wormwood wild resource with the major organs stored
It is abundant, but artemislnin content is not high.The change of artemislnin content is extremely notable in the sweet wormwood kind of different geographical, bud, wherein with
The bud artemislnin content of Chongqing region tenth of the twelve Earthly Branches sun and Xiushan Mountain sweet wormwood is optimal.In order to reduce pharmaceutical factory extract qinghaosu pharmacy into
This, except traditional breeding way is utilized, Recent study improves the artemislnin content of sweet wormwood using gene engineering method, cultivates Gaoqing
The bioengineering kind of artemisin content turns into focus.
Sweet wormwood primary medicinal component and effect qinghaosu chemical constitution contain peroxy-radical, are a kind of sesquiterpene lactones, blue or green
A variety of derivatives of artemisin such as double hydrogen sweet wormwood rope (dihydroanemisin), Artesunate (anesunatc), Artemethers
(ancmether), arteether (aneether), is that the active drug researchs for treating malaria show that dihydroartemisinine is to rat
The selective CDCC of C6 cells also can induce lung cancer cell line PC-14 cells and SPC-A-1 Apoptosis sweet wormwood ambers
Ester has inhibitory action to Human Large Intestine Carcinoma Cells, can suppress proliferation of colorectal cancer cells and promote apoptosis.Artemether, arteether and blue or green punt-pole
Amber ester is acted on after tumour cell, there is correlation between the mRNA of expression and its inhibitory action to tumour cell.As a result show
The drug action mode and the not the same of known cancer therapy drug for showing artemisinin derivative are made with to artemisinin-based drug pharmacology
With the continuous deep people of research, having proven to it has anti-fibrosis, resisting toxoplasmosis, antimalarial, anti-pregnant, anti-schistosome, tumor cytotoxicity
Although effect such medicine effects such as property and anti-arrhythmia are extensive, its mechanism of action, feature and application are still in primary rank
Section, research shows that qinghaosu can mitigate the injury of lungs of rats with sepsis by suppressing lung tissue local inflammatory response.Sweet wormwood is also
Containing volatile ingredient, mainly volatile oil include punt-pole ketone, sesquialter Chu alcohol, different punt-pole ketone, cloves alkene, caryophyllene oxide, by oil
The composition such as essence, trip's alkene, levo-camphor, borneol wherein, punt-pole ketone, camphor, the different punt-pole ketone of cloves alkene, the higher volatile oil of borneol equal size
With anti-inflammation, antipyretic-antalgic, it is relieving cough and asthma the effects such as.
The synthesis of sweet wormwood terpenoid mainly passes through two approach of MVA and DXP, i.e. mevalonic acid (mevalonate
Pathway, MVA) approach and 1- deoxy-D-xyluloses sugar 5- phosphate synthases (1-deoxy-D-xylulose-5-phosphate
Synthase, DXS) or methyl erythrite 4- phosphoric acid (methylerythritol 4-phosphatepathway, MEP) DXP ways
Footpath.3-hydroxy-3-methylglutaryl-coenzyme A reductase (3-hydroxy-3-methyl glutaryl coenzyme A
Reductase, HMGR) it is the important rate-limiting enzyme of MVA approach, and 1- deoxy-D-xyluloses sugar 5- phosphate synthases (1-deoxy-D-
Xylulose-5-phosphate synthase, DXS) be DXP approach key enzyme.In 3 kinds of direct precursor material farnesyl-s
Diphosphonic acid (farnesyl diphosphate, FPP), geranyl diphosphonic acid (geranyl diphosphate, GPP) and spiceleaf
After basic note phyllopodium diphosphonic acid (geranyl geranyl diphosphate, GGPP) synthesis, i.e., into terpenes building-up process,
Several terpene synthases (terpene synthase, TPS) play an important role in this process.GPP is in monoterpene synthase
The lower generation monoterpene (monoterpene, C10) of (monoteterpene synthase) effect, FPP is in sesquiterpene synthase
The lower generation sesquiterpene (sesquiterpene, C15) of (sesquiterpene synthase, SPS) effect, GGPP is in diterpene
Into two terpenes (diterpene, C20) under alkene synthase (diterpene synthase) effect.
Qinghaosu is the topmost effective clinical application for treating malaria, in addition, pharmacological further investigation shows, it is blue or green
Artemisin and its derivative are a kind of natural drugs of broad spectrum activity.Research shows, has more than 1,000,000 people in the world every year and dies from malaria
Disease.Plasmodium drug resistance so that the effect of drugs of malarial drug chloroquine is limited, and qinghaosu has low toxicity to malaria and quick-acting
Feature.Current qinghaosu is to generally acknowledge effective treatment malaria choice drug, and this causes the raw material sweet wormwood demand for extracting qinghaosu
Amount is very big.Sweet wormwood industrial seedling rearing can save the cost of plantation sweet wormwood, and sweet wormwood graft technology of the invention is by sweet wormwood factory
Change nursery to be possibly realized.Wild sweet wormwood flower is artemislnin content highest organ, is also pharmaceutical factory to reduce extraction qinghaosu
Cost, be most ready use raw material part.
But only sweet wormwood plant leaf is regulated and controled in the prior art, it have ignored bud as artemislnin content most
Organ.Therefore, it should study and chemical regulation is carried out to bud, bud is expanded weightening while improving its artemislnin content.
The content of the invention
Present invention aim to address the problem of sweet wormwood flower chemical regulation.
To realize that the technical scheme that the object of the invention is used is a kind of such, weight and sweet wormwood for improving sweet wormwood flower
The method of cellulose content, it is characterised in that comprise the following steps：
1) sweet wormwood sowing and management
1.1) sweet wormwood was collected seed in the last ten-days period in October in seed up to the maturity period, after seed collecting, took out seed sieving, was removed thick miscellaneous
Matter, is cleaned, and is spread in shady and cool ventilation, is dried in the air untill seeds of southernwood kind skin blacks；
1.2) seeds of southernwood sterilization and vernalization
With 50% carbendazim+1：The many potassium permanganate of Mil+0.48% warm water of thunder of 1500 times of liquid are to the stock kind before sowing
Son sterilization, the temperature of 0.48% potassium permanganate warm water is 41 DEG C, 2 hours of disinfecting time, and then clear water is cleaned, and gauze wrapped exists
Vernalization 30 hours in 33 DEG C of insulating boxs；Before sowing 2% (NH4) is added with 3% calcium superphosphate2SO420 hours of immersion kind, dry in the shade；
And rejecting has the viral mouldy empty flat seed that is off color；
1.3) sweet wormwood sowing nursery
Selection landform is flat, physical features is higher, well-drained open ground, the gentle slope of l-3 degree；Not only preferably pour water but also preferably arrange
Water, soil fertility and quality select that the deep chad of soil layer is few, and short texture is fertile, water penetration aeration good light loam or
Sandy loam or loamy sand, as nursery soil medium silt and sticky content between 18%-28%；Soil acidity or alkalinity PH6.4-
7.6, i.e. neutral, subacidity or subalkaline soil；Loose sandy loam plot should be selected sweet wormwood seedling bed, it is sunny,
10-20 DEG C of slope land；Hoe the fields to deep-cut and turn over 15-22 centimeters；Dig ditch wide 50 centimeters into railway carriage or compartment, it is long to do railway carriage or compartment according to landform, 5 meters long,
Railway carriage or compartment face will be put down, and spill liquid dung；After Seedlings nursery bed is carried out, sprinkle and apply farm manure, every mu of 15-18 load when not doing not wet, uses hoe
Head refinement soil, smoothes out surface management, sprayer spray disinfectant liquid disinfectant, arch membrane covering；With vegetable booth thermal insulation, resist down
Cold spell in spring；
1.4) sweet wormwood is after planting managed
Temperature >=18 DEG C before stock seed sprouting, while not higher than 25 DEG C, stock seedling excessive growth is prevented, need not before emerging
Water, the insulation of covering with plastic film water conservation 7 days after emerging, removes mulch film, suitably watered, see it is dry see wet, then 0.5% permanganic acid
The carbendazim of potassium solution+1% sprayer sprays disinfectant liquid disinfectant, arch membrane covering；With vegetable booth thermal insulation, cold spell in later spring is resisted；
2) field planting
When late April-the first tenday period of a month in May seedling grows 2-3 branch, the healthy and strong seedling replanting without disease pest of selection is to greatly
Field, seedling cultivation suitably selects sandy loam, and sunny, draining is unobstructed；Require to turn over plow harrow in small, broken bits, removal of impurities seedling field planting
After grass, smooth-going is arranged, farmyard manure or farm manure 2200-2600kg are become thoroughly decomposed in fertilising requirement and phosphate fertilizer 40-55kg, K fertilizer 5kg make bottom
Fertilizer, blinding makees ridge；Surrounding is ditched, 18-22 centimeters of ditch depth, 32-45 centimeters of furrow width, is drained flooded fields beneficial to draining；
Seedling and crop field are with 1 before transplanting：The bacterium of liquor potassic permanganate more than+1% of many Mils+0.5% of thunder of 1500 times of liquid
Clever disinfection；Seedling height is at 15 centimeters or so, and crop field is arrived in seedling replanting field planting, selects overcast and rainy；Seedling field planting is close
Spend for 0.8 × 0.8 meter, every mu 850-950 plants of seeding row spacing；Plant it is dilute it is close can be adjusted according to soil fertility, it is fairly simple；Children
Seedling diseases pest control：With Cercobinm medicine liquid spray prevention stem rot, downy mildew, powdery mildew and anthracnose, spread
This this granule of Xerox sprays the anti-aphid of decis decoction and black cutworm, using Imidacloprid Contol ofCotton aphid；
1. temperature；Covered after transplanting with film, after transplanting in 7 days, day temperature requires control in 25-27 DEG C, evening 22-
25 DEG C, maximum temperature is no more than 18-25 DEG C of temperature after 32 DEG C, 2 days, transplanted seedling temperature treatment and conventional sweet wormwood seedling after one week
Together；Sooner or later each ventilation is once；2. humidity；Cover film keeps moisture, humidity requirement 86%-95% in 7 days, optic lobe piece after transplanting
Here no wither, appropriate specking water, and 50% carbendazim solution is sprayed, film is removed after 2 days, transplanted seedling moisture management and routine after one week
Sweet wormwood seedling is same；3. illumination；It is required that sufficient, the sun greatly, with Shading net covering, removes Shading net covering, one week very much after 5 days
Afterwards according to normal nursery bed management；4. fertilizer；It is same with conventional sweet wormwood nursery bed management；
Mixed solution is sprayed in the knot flower bud phase of sweet wormwood；The concentration of each component is in the mixed solution：Zinc sulfite
10g/L, tyrosinase 15 g/L, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, sodium diethyldithiocarbamate 5g/L, two
Methyl sulfoxide 2g/L, 6-BA (6- benzyls aminoadenine) 200mmol/L, 20 milligrams per liter of gibberellin, β-heteroauxin 500mg/
L, Vc (vitamin C) 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 300, the mg/litre of cycocel (2-chloroethyl trimethyl ammonium chloride) 20.
A kind of method for the weight and artemislnin content for improving sweet wormwood flower, is prepared in mixed solution, the mixed solution
The concentration of each component is：Zinc sulfite 10g/L, tyrosinase 15 g/L, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, diethyl
Base nabam 5g/L, dimethyl sulfoxide (DMSO) 2g/L, 6-BA (6- benzyls aminoadenine) 200mmol/L, gibberellin 20
Milligrams per liter, β-heteroauxin 500mg/L, Vc 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 300, the mg/litre of cycocel 20.In sweet wormwood
Knot flower bud phase spray above-mentioned mixed solution.
A kind of method for the weight and artemislnin content for improving sweet wormwood flower, prepares A liquid and B liquid.In the A liquid, each
Constituent concentration is：Zinc sulfite 10g/L, tyrosinase 15 g/L, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, the sulphur of diethyl two
For carbamic acid sodium 5g/L, dimethyl sulfoxide (DMSO) 2g/L.In the B liquid, each constituent concentration is：6-BA concentration 200mmol/L, it is red
20 milligrams per liter of mycin, β-heteroauxin 1000mg/L, Vc 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 300, the mg/litre of cycocel 25,
Dimethyl sulfoxide (DMSO) 2g/L.Successively above-mentioned A liquid and B liquid are sprayed in the knot flower bud phase of sweet wormwood.
A kind of method for the weight and artemislnin content for improving sweet wormwood flower, prepares A liquid and B liquid.In the A liquid, each
Constituent concentration is：Zinc sulfite 10g/L, tyrosinase 15 g/L, 20 milligrams per liter of gibberellin, β-heteroauxin 100mg/L, diformazan
Base sulfoxide 2g/L.In the B liquid, each constituent concentration is：6-BA concentration 200mmol/L, Vc 5mg/L, 500 milligrams of ethephon (CEPHA),2-(chloroethyl) phosphonic acid/
Rise, the mg/litre of cycocel 20, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, sodium diethyldithiocarbamate 5g/L,
Dimethyl sulfoxide (DMSO) 2g/L.Successively above-mentioned A liquid and B liquid are sprayed in the knot flower bud phase of sweet wormwood.
The solution have the advantages that unquestionable.It is low that the chemical regulation method solves sweet wormwood plant artemislnin content
Bottleneck problem, is further illustrated by three following embodiments.
Embodiment
With reference to embodiment, the invention will be further described, but should not be construed above-mentioned subject area of the invention only
It is limited to following embodiments.Without departing from the idea case in the present invention described above, according to ordinary skill knowledge and used
With means, various replacements and change are made, all should be included within the scope of the present invention.
What deserves to be explained is, the method for the measure artemislnin content of three below embodiment is：
Artemislnin content measure use instrument " general analysis is general " high performance liquid chromatograph (P680A quarternary low pressure gradient pumps,
PDA2100 PDADs, TCC2100 column ovens, Chromeleon chromatographic work stations).Chromatographic condition：This experiment is adopted
It is chromatographic column:Dikma Kromasil C18column (10 μm, 200mm × 4.5mm), mobile phase:Methanol 0.01mol/L
Acetate/acetic buffer solution (pH=6.0) (65: 35: 1), flow velocity:0.8ml/min, Detection wavelength:250nm, column temperature:32℃.
Compare (implantation methods are as the sweet wormwood for spraying medicine) and the test sample (sweet wormwood planted according to embodiment method
Bud) solution preparation：Put in apparatus,Soxhlet's, plus petroleum ether (30~60 DEG C) 35ml, refluxing extraction 5h, extract solution is taken,
Water bath method, residue absolute ethyl alcohol, which dissolves and is settled in 10ml measuring bottles, to be shaken up, and takes 1mL to put in 10ml measuring bottles, adds 0.2%
Sodium hydroxide solution 4ml, shakes up.It is to be measured after being diluted with 0.08mol/L hac buffers.
The making of standard curve
Sample introduction 1 μ L, 3 μ L, 5 μ L, 7 μ are distinguished into above-mentioned control (standard sweet wormwood solution) under the conditions of corresponding HPLC chromatogram
L, 9 μ L, 11 μ L record collection of illustrative plates and chromatographic parameter, respectively with peak area (Y) to standard items content (X, μ g) fit regression curve.
Research finds that linearity curve regression equation of the artemisinine in 5-25 μ g ranges is in the present invention:Y=1.311e+OOOX+
5.01e+000, R=0.98853446.Weigh and use electronic balance.4 sweet wormwood flower chemical regulations (are divided into 3 embodiments)
In addition, the method for sweet wormwood cultivation may be referred to following method：
1 sweet wormwood is sowed and managed
1.1 sweet wormwoods were collected seed in the last ten-days period in October in seed up to the maturity period, after seed collecting, were taken out seed sieving, were removed thick impurity,
Clean, spread in shady and cool ventilation, dry in the air untill seeds of southernwood kind skin blacks.
1.2 seeds of southernwood are sterilized and vernalization
With 50% carbendazim+1：Thunder 41 DEG C of warm water of many potassium permanganate of Mil+0.48% of 1500 times of liquid are to the anvil before sowing
Wooden seed disinfection, 2 hours of time, then clear water is clean, gauze wrapped vernalization 30 hours in 33 DEG C of insulating boxs.Sow eve
2% (NH is added with 3% calcium superphosphate4)2SO420 hours of immersion kind, dry in the shade.And reject have virus, be off color, it is mouldy
Empty flat seed.
1.3 sweet wormwoods sow nursery
Select landform it is flat, physical features is higher, well-drained open ground, the gentle slope of l-3 degree it is optimal.Not only preferably poured water but also suitable
Draining, soil fertility and quality typically select that the deep chad of soil layer is few, and short texture is fertile, water penetration aeration good light earth
Soil or sandy loam or loamy sand, as nursery soil medium silt and sticky content between 18%-28%.Soil acidity or alkalinity
PH6.4-7.6 is that is, general with neutral, subacidity or subalkaline soil optimum.Loose sand should be selected sweet wormwood seedling bed
Loam plot, sunny, 10-20 DEG C of slope land draining is preferable.Hoe the fields to deep-cut and turn over 15-22 centimeters.Dig ditch wide by 50 into railway carriage or compartment
Centimetre, does that railway carriage or compartment is long according to landform, and general 5 meters long, railway carriage or compartment face will be put down, and spill liquid dung.After Seedlings nursery bed is carried out, sprinkle and apply farm manure (often
Mu 15-18 loads), when not doing not wet, soil is refined using hoe, surface management is smoothed out, sprayer spray sterilization decoction disappears
Poison, arch membrane covering.With vegetable booth thermal insulation, cold spell in later spring is resisted.
1.4 sweet wormwoods are after planting managed
Temperature >=18 DEG C before stock seed sprouting, while not higher than 25 DEG C, stock seedling excessive growth is prevented, need not before emerging
Water, the insulation of covering with plastic film water conservation 7 days after emerging, removes mulch film, suitably watered, see it is dry see wet, then 0.5% permanganic acid
The carbendazim of potassium solution+1% sprayer sprays disinfectant liquid disinfectant, arch membrane covering.With vegetable booth thermal insulation, cold spell in later spring is resisted.
2 field plantings
When late April-the first tenday period of a month in May seedling grows 2-3 branch, the healthy and strong seedling replanting without disease pest of selection is to greatly
Field, seedling cultivation suitably selects sandy loam, and sunny, draining is unobstructed.Require to turn over plow harrow in small, broken bits, removal of impurities seedling field planting
After grass, smooth-going is arranged, farmyard manure or farm manure 2200-2600kg are become thoroughly decomposed in fertilising requirement and phosphate fertilizer 40-55kg, K fertilizer 5kg make bottom
Fertilizer, blinding makees ridge.Surrounding is ditched, 18-22 centimeters of ditch depth, 32-45 centimeters of furrow width, is drained flooded fields beneficial to draining.
Seedling and crop field are with 1 before transplanting：The bacterium of liquor potassic permanganate more than+1% of many Mils+0.5% of thunder of 1500 times of liquid
Clever disinfection.Seedling height is at 15 centimeters or so, and crop field is arrived in seedling replanting field planting, is preferably selected overcast and rainy.Seedling crop field kind
Density in planting is 0.8 × 0.8 meter of seeding row spacing, every mu 850-950 plants.Plant it is dilute it is close can be adjusted according to soil fertility, relatively it is simple
It is single.The seedling prevention and control of plant diseases, pest control：With the bactericide such as thiophanate methyl medicine liquid spray prevention stem rot, downy mildew, powdery mildew and charcoal
Subcutaneous ulcer disease, spreads fertilizer over the fields Le Siben granules or sprays the anti-aphid of decis decoction and black cutworm, using Imidacloprid Contol ofCotton aphid.
1. temperature.Cover film is used after transplanting, after transplanting in 7 days, day temperature requires control in 25-27 DEG C, evening 22-
25 DEG C, maximum temperature is no more than 18-25 DEG C of temperature after 32 DEG C, 2 days, transplanted seedling temperature treatment and conventional sweet wormwood seedling after one week
Together.Sooner or later each ventilation is once.2. humidity.Moisture is kept with cover film, humidity requirement 86%-95%, optic lobe in 7 days after transplanting
Piece is withered here no, appropriate specking water, and sprays 50% carbendazim solution, and film is removed after 2 days, after one week transplanted seedling moisture management with often
Advise sweet wormwood seedling same.3. illumination.It is required that sufficient, the sun greatly, with Shading net covering, removes Shading net covering, one very much after 5 days
According to normal nursery bed management after week.4. fertilizer.It is same with conventional sweet wormwood nursery bed management.
3 determine artemislnin content (above method)
Embodiment 1：
Pharmaceutical formulation is as follows：
Zinc sulfite 10g/L, tyrosinase 15 g/L, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, diethyl-dithio ammonia
Base sodium formate 5g/L, dimethyl sulfoxide (DMSO) 2g/L, 6-BA concentration 200mmol/L, 20 milligrams per liter of gibberellin, β-heteroauxin
500mg/L, Vc 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 300, the mg/litre of cycocel 20.Mixed and sprayed with 28 DEG C of clear water.
Carried above using control mode (not spraying medicine, mode of the other conditions with embodiment above is identical) or embodiment
To mode cultivate sweet wormwood.Spraying time：(before blooming 1 week) is sprayed when sweet wormwood has about 1% bud knot flower bud, and sweet wormwood is planted
All stalk blades of strain are drenched.Select dew on, sweet wormwood plant to be sprayed after all finishing thirty in the fine day morning 10.After spraying, people
Do not shuttled inside sweet wormwood crop field.
When bud has 8 maturation, harvesting sweet wormwood flower determines artemislnin content, is weighed after drying.Under identical cultivation condition, not
The sweet wormwood flower for spraying medicine is compareed, and is repeated 3 times measure.After measured, compared with the control, bud artemislnin content increase
20%-40%, bud weight increase 30%-42%.
The artemislnin content % of table 1
Bud weight (unit g) of the table 2 per 1kg sweet wormwoods
Embodiment 2
Pharmaceutical formulation is as follows：
For the first time (A liquid)：Zinc sulfite 10g/L, tyrosinase 15 g/L, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, two
Sodium diethyldithiocarbamate 5g/L, dimethyl sulfoxide (DMSO) 2g/L, second (B liquid)：6-BA concentration 200mmol/L, gibberellin
20 milligrams per liter, β-heteroauxin 1000mg/L, Vc 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 300, the mg/litre of cycocel 25, diformazan
Base sulfoxide 2g/L, is mixed with 28 DEG C of clear water and sprayed.
Spraying time point 2 times, is sprayed on the 1st respectively at August 20 days and September.All stalk blades of sweet wormwood plant are drenched.
Select dew on, sweet wormwood plant to be sprayed after all finishing thirty in the fine day morning 10.After spraying, people not inside sweet wormwood crop field back and forth
Shuttle.
When bud has 8 maturation, harvesting sweet wormwood flower determines artemislnin content, is weighed after drying, not spray the green grass or young crops of medicine
Wormwood artemisia bud is compareed, and is repeated 3 times measure.After measured, compared with the control, bud artemislnin content increase 32%-52%, bud
Weight increases 38%-47%.In control mode, medicine is not sprayed, mode of the other conditions with embodiment above is identical.
The artemislnin content % of table 3
Bud weight (unit g) of the table 4 per 1kg sweet wormwoods
Embodiment 3
Pharmaceutical formulation is as follows：
For the first time (A liquid)：Zinc sulfite 10g/L, tyrosinase 15 g/L, 20 milligrams per liter of gibberellin, β-heteroauxin
100mg/L, dimethyl sulfoxide (DMSO) 2g/L,
Second (B liquid)：6-BA concentration 200mmol/L, Vc 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 500,20 milligrams of cycocel/
Rise, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, sodium diethyldithiocarbamate 5g/L, dimethyl sulfoxide (DMSO) 2g/L, with
28 DEG C of clear water, which are mixed, to be sprayed.
Spraying time point 2 times, is sprayed on the 1st respectively at August 20 days and September.All stalk blades of sweet wormwood plant are drenched.
Select dew on, sweet wormwood plant to be sprayed after all finishing thirty in the fine day morning 10.After spraying, inside people no longer sweet wormwood crop field back and forth
Shuttle.
When bud has 8 maturation, harvesting sweet wormwood flower determines artemislnin content, is weighed after drying, not spray the green grass or young crops of medicine
Wormwood artemisia bud is compareed, and is repeated 3 times.After measured, compared with the control, bud artemislnin content increase 38%-56%, bud weight
Increase 42%-52%.In control mode, medicine is not sprayed, mode of the other conditions with embodiment above is identical.
The artemislnin content % of table 5
Bud weight (unit g) of the table 6 per 1kg sweet wormwoods
In summary, the method for the weight and artemislnin content of the chemical regulation sweet wormwood flower that the present invention is provided, operation letter
Single reliable, cost is low, is easily received by medicinal herb grower, remarkable in economical benefits.Bud weight and artemislnin content are improved largely, and push away
Extensively have a extensive future.
Claims (3)
1. a kind of method for the weight and artemislnin content for improving sweet wormwood flower, it is characterised in that：Mixed solution is prepared, is mixed molten
The concentration of the component of each in liquid is：
Zinc sulfite 10g/L, tyrosinase 15 g/L, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, diethyl-dithio amino first
Sour sodium 5g/L, dimethyl sulfoxide (DMSO) 2g/L, 6-BA (6- benzyls aminoadenine) 200mmol/L, 20 milligrams per liter of gibberellin, β-Yin
Indolylbutyric acid 500mg/L, Vc 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 300, the mg/litre of cycocel 20；
Above-mentioned mixed solution is sprayed in the knot flower bud phase of sweet wormwood.
2. a kind of method for the weight and artemislnin content for improving sweet wormwood flower, it is characterised in that：Prepare A liquid and B liquid；
In the A liquid, each constituent concentration is：Zinc sulfite 10g/L, tyrosinase 15 g/L, farnesyl diphosphonic acid 5g/L, first hydroxyl penta
Sour 5g/L, sodium diethyldithiocarbamate 5g/L, dimethyl sulfoxide (DMSO) 2g/L；
In the B liquid, each constituent concentration is：6-BA concentration 200mmol/L, 20 milligrams per liter of gibberellin, β-heteroauxin
1000mg/L, Vc 5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 300, the mg/litre of cycocel 25, dimethyl sulfoxide (DMSO) 2g/L；
Successively above-mentioned A liquid and B liquid are sprayed in the knot flower bud phase of sweet wormwood.
3. a kind of method for the weight and artemislnin content for improving sweet wormwood flower, it is characterised in that：Prepare A liquid and B liquid；
In the A liquid, each constituent concentration is：Zinc sulfite 10g/L, tyrosinase 15 g/L, 20 milligrams per liter of gibberellin, β-Yin
Indolylbutyric acid 100mg/L, dimethyl sulfoxide (DMSO) 2g/L；
In the B liquid, each constituent concentration is：6-BA concentration 200mmol/L, Vc5mg/L, the mg/litre of ethephon (CEPHA),2-(chloroethyl) phosphonic acid 500 is short strong
Plain 20 mg/litres, farnesyl diphosphonic acid 5g/L, mevalonic acid 5g/L, sodium diethyldithiocarbamate 5g/L, dimethyl is sub-
Sulfone 2g/L；
Successively above-mentioned A liquid and B liquid are sprayed in the knot flower bud phase of sweet wormwood.
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|---|---|---|---|---|
| CN1985572A (en) * | 2006-12-28 | 2007-06-27 | 上海交通大学 | Salt treatment process for raising artemisinin content in sweet wormwood herb |
| CN101011007A (en) * | 2007-02-08 | 2007-08-08 | 湖南诺德生物科技有限公司 | Sweet wormwood cultivation method |
| CN101171915A (en) * | 2007-11-15 | 2008-05-07 | 上海交通大学 | Method for improving arteannuin content in abrotanum with abscisic acid process |
| CN101785407A (en) * | 2010-02-25 | 2010-07-28 | 唐罗欧 | Method for improving artemisinine content in sweet wormwood herb by methyl jasmonate treatment |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1985572A (en) * | 2006-12-28 | 2007-06-27 | 上海交通大学 | Salt treatment process for raising artemisinin content in sweet wormwood herb |
| CN101011007A (en) * | 2007-02-08 | 2007-08-08 | 湖南诺德生物科技有限公司 | Sweet wormwood cultivation method |
| CN101171915A (en) * | 2007-11-15 | 2008-05-07 | 上海交通大学 | Method for improving arteannuin content in abrotanum with abscisic acid process |
| CN101785407A (en) * | 2010-02-25 | 2010-07-28 | 唐罗欧 | Method for improving artemisinine content in sweet wormwood herb by methyl jasmonate treatment |
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| 青蒿栽培技术研究;韦中强等;《现代中药研究与实践》;20110315;第25卷(第2期);第6-8页 * |
| 青蒿栽培管理技术;田海龙;《南方农业》;20110315;第5卷(第3期);第13-15页 * |
| 青蒿高产栽培技术;苏世东等;《中国农技推广》;20071225(第12期);第28-29,10页 * |
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