CN109874810A - A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase - Google Patents

A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase Download PDF

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CN109874810A
CN109874810A CN201910093533.1A CN201910093533A CN109874810A CN 109874810 A CN109874810 A CN 109874810A CN 201910093533 A CN201910093533 A CN 201910093533A CN 109874810 A CN109874810 A CN 109874810A
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conopomorpha sinensis
sinensis bradley
adult
ethanol extract
bradley
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CN109874810B (en
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姚琼
全林发
董易之
陈炳旭
林少源
冯日碧
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Plant Protection Research Institute Guangdong Academy of Agricultural Sciences
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Abstract

The invention discloses a kind of Conopomorpha sinensis Bradley adult stages to cooperate with preventing control method with the ovum phase.It is to spray litchi and longan fruit tree in the Conopomorpha sinensis Bradley adult stage with Conopomorpha sinensis Bradley adult repellant;In the Conopomorpha sinensis Bradley ovum phase, fruit tree is sprayed with Conopomorpha sinensis Bradley ovicide.Conopomorpha sinensis Bradley adult stage and ovum phase proposed by the present invention collaboration preventing control method are can not to carry out this feature of prevention and control for Conopomorpha sinensis Bradley larval phase and pupa time by laboratory and orchard verification test, research and develop the worm adult stage and with the ovum phase cooperate with preventing control method.Pest population quantity can be effectively controlled using method of the invention, the use of chemical pesticide is greatly reduced, not only ensure that control efficiency, but also reduces the influence to environment to the maximum extent, the yield and quality for increasing substantially litchi and longan reaches litchi and longan safety in production purpose.

Description

A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase
Technical field
The invention belongs to field of agricultural production technologies, are related to a kind of pest and disease damage preventing control method, and in particular to a kind of lichee base of a fruit The moth adult stage cooperates with preventing control method with the ovum phase.
Background technique:
Lichee and longan are the rare fruit of advantage in China, and the cultivated area and fruit yield of the two rank first in the world Position.Conopomorpha sinensis Bradley (Conopomorpha sinensis Bradley) be litchi and longan cultivation in take place frequently, fulminant moth, The worm is caused harm with larva, is that the pest of litchi and longan industry " zero tolerance " is anti-up to 60% or more when borer fruit rate is serious Control object.Meanwhile Conopomorpha sinensis Bradley is always the prevention and control problem in fruit tree pest insect, has only and is largely administered repeatedly just in its adult stage It can guarantee control effect.However repeatedly a large amount of uses of chemical pesticide cause a series of problems, comprising: pest resistance to insecticide is accelerated to produce It is raw, environmental pollution is aggravated, causes litchi and longan agriculture residual exceeded and influences fruit quality and domestic outlet etc..Conopomorpha sinensis Bradley has Brill moth feature and unique pests occurrence rule can not carry out prevention and control in the worm larval phase and pupa time.
Summary of the invention:
For the defects in the prior art, provide a kind of Conopomorpha sinensis Bradley adult stage with the ovum phase cooperates with anti-prosecutor to the present invention Method, the preventing control method are killed ovum in the Conopomorpha sinensis Bradley ovum phase, by interfering its oviposition in the Conopomorpha sinensis Bradley adult stage to have Effect control Conopomorpha sinensis Bradley population quantity, reduces the use of chemical pesticide.
The Conopomorpha sinensis Bradley adult stage of the invention cooperates with preventing control method with the ovum phase, which is characterized in that (1) Conopomorpha sinensis Bradley at The worm phase sprays litchi and longan fruit tree with Conopomorpha sinensis Bradley adult repellant;(2) it the Conopomorpha sinensis Bradley ovum phase, is killed with Conopomorpha sinensis Bradley Fruit tree is sprayed in ovum agent.Different agents are applied to carry out prevention and control, Conopomorpha sinensis Bradley adult respectively in Conopomorpha sinensis Bradley adult stage and ovum phase Repellant repellent Conopomorpha sinensis Bradley adult, makes it not lay eggs on lichee, and Conopomorpha sinensis Bradley ovicide kills Conopomorpha sinensis Bradley Worm's ovum, by cooperateing with prevention and control in the crucial developmental stages of the worm two.
It is preferred that the Conopomorpha sinensis Bradley adult repellant comprising Wedelia trilobata ethanol extract and lantana Ethanol extract.The Conopomorpha sinensis Bradley adult repellant is plant source oviposition detterent agent, is a kind of green medicament.
It is preferred that the Conopomorpha sinensis Bradley adult repellant is prepared by the following method, trilobated leaf is added in water Wedelia chinensis cauline leaf ethanol extract and lantana cauline leaf ethanol extract.
Further preferably, the Conopomorpha sinensis Bradley adult repellant is prepared by the following method, in every 100g water Middle addition 10-30g Wedelia trilobata cauline leaf ethanol extract and 10-30g lantana cauline leaf ethanol extract, thus obtain litchi Branch base of a fruit moth adult repellant.Tender tip and the fruit position of litchi and longan fruit tree are sprayed with the Conopomorpha sinensis Bradley adult repellant, The amount of spraying is 5L/ plants.
It is preferred that the Conopomorpha sinensis Bradley ovicide is the preparation for killing Conopomorpha sinensis Bradley ovum, it such as include diflubenzuron and secondary Octanol polyoxyethylene ether, is further preferably prepared by the following method: 0.01g diflubenzuron and 20g Zhong Xin being added in every 100g water Alcohol polyoxyethylene ether.Fruit tree is sprayed with Conopomorpha sinensis Bradley ovicide, the amount of spraying is 5L/ plants.
Generally from the Conopomorpha sinensis Bradley adult stage by the Conopomorpha sinensis Bradley ovum phase general two days or so, thus Conopomorpha sinensis Bradley at The worm phase sprays litchi and longan fruit tree with Conopomorpha sinensis Bradley adult repellant, and the amount of spraying is (Conopomorpha sinensis Bradley ovum after 5L/ plants, 2 days Phase), fruit tree is sprayed with Conopomorpha sinensis Bradley ovicide, the amount of spraying is 5L/ plants.Implement frequency are as follows: from lichee fruiting early period to receipts fruit Period, every month implement 1 time.
Wedelia trilobata cauline leaf ethanol extract of the invention, lantana cauline leaf ethanol extract, fragrant eupatorium herb cauline leaf second Alcohol extracting thing and palace powder mountain ebony cauline leaf ethanol extract the preparation method is as follows:
Alcohol steep is used after dry Wedelia trilobata cauline leaf is crushed, and obtains three after the concentrated drying of ethanol extract Decomposite leaf wedelia chinensis cauline leaf ethanol extract.
Alcohol steep is used after dry lantana cauline leaf is crushed, and obtains lantana stem after the concentrated drying of ethanol extract Leaf ethanol extract.
Alcohol steep is used after dry fragrant eupatorium herb cauline leaf is crushed, and obtains aircraft grass blade after the concentrated drying of ethanol extract Leaf ethanol extract.
Alcohol steep is used after dry palace powder mountain ebony cauline leaf is crushed, and obtains palace powder after the concentrated drying of ethanol extract Mountain ebony cauline leaf ethanol extract.
Compared with prior art, of the invention to have the advantage that
(1) plant source Conopomorpha sinensis Bradley adult repellant proposed by the present invention is produced by interference adult stage Conopomorpha sinensis Bradley Ovum behavior can effectively reduce and fall ovum amount on litchi and longan fruit, mitigate the prevention and treatment pressure in later period.
(2) its group of Conopomorpha sinensis Bradley ovicide proposed by the present invention is divided into diflubenzuron and the mixing of sec-octyl alcohol polyoxyethylene ether Object, the two are respectively insect growth regulator, IGR and less toxic bleeding agent, environmental safety height, and degradation speed lower using concentration Fastly, it can promote the use of a large area.
(3) Conopomorpha sinensis Bradley adult stage and ovum phase collaboration preventing control method proposed by the present invention is tested by laboratory and orchard Confirmatory test can not carry out this feature of prevention and control for Conopomorpha sinensis Bradley larval phase and pupa time, research and develop worm adult stage and ovum phase Cooperate with preventing control method.Pest population quantity can be effectively controlled using method of the invention, the use of chemical pesticide is greatly reduced, Not only it ensure that control efficiency, but also reduce the influence to environment to the maximum extent, increase substantially the yield and product of litchi and longan Matter reaches litchi and longan safety in production purpose.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Wedelia trilobata cauline leaf ethanol extract that following embodiment uses, lantana cauline leaf ethanol extract, aircraft Grass blade leaf ethanol extract and palace powder mountain ebony cauline leaf ethanol extract the preparation method is as follows:
It is used alcohol steep 24 hours after dry Wedelia trilobata cauline leaf is crushed, after the concentrated drying of ethanol extract Obtain Wedelia trilobata cauline leaf ethanol extract.
It is used alcohol steep 24 hours after dry lantana cauline leaf is crushed, obtains horse after the concentrated drying of ethanol extract Tassel pellet cauline leaf ethanol extract.
It uses alcohol steep 24 hours after dry fragrant eupatorium herb cauline leaf is crushed, is flown after the concentrated drying of ethanol extract Machine grass blade leaf ethanol extract.
It is used alcohol steep 24 hours after dry palace powder mountain ebony cauline leaf is crushed, after the concentrated drying of ethanol extract To palace powder mountain ebony cauline leaf ethanol extract.
Embodiment 1:
Medicament A: 20g Wedelia trilobata cauline leaf ethanol extract and 20g lantana cauline leaf ethanol extract are added to In 100g water, mixes and obtain medicament A.
Medicament B: 20g fragrant eupatorium herb cauline leaf ethanol extract and the palace 20g powder mountain ebony cauline leaf ethanol extract are added to In 100g water, mixes and obtain medicament B.
Medicament C: 40g Wedelia trilobata cauline leaf ethanol extract is added in 100g water, is mixed and is obtained medicament C.
Medicament D: 40g lantana cauline leaf ethanol extract is added in 100g water, is mixed and is obtained medicament D.
Laboratory test: using medicament A, medicament B, medicament C and medicament D as test group medicament, using clear water as control group medicament.
Choice tests: after the lichee fresh fruit dried is impregnated 10s in four kinds of medicaments (A, B, C and D) and clear water respectively It takes out, to lay eggs, paper is wrapped up.5d or so is in production after each 10 pairs of emergence of dependent insect cage (30cm × 30cm × 30cm) access The Conopomorpha sinensis Bradley adult (♀: ♂=1:1) of ovum peak period, by medicament A (4 fruits) and clear water (4 fruits) treated lichee fresh fruit Random alignment is put into the same cage, and by medicament B (4 fruits) and clear water (4 fruits), treated, and lichee fresh fruit random alignment is put Enter in another cage, by medicament C (4 fruits) and clear water (4 fruits), treated, and lichee fresh fruit random alignment is put into a cage, By medicament D (4 fruits) and clear water (4 fruits), treated, and lichee fresh fruit random alignment is put into another cage, in four cages respectively It is put into 10% hydromel of mass fraction, it is allowed to lay eggs, removes lichee fresh fruit afterwards for 24 hours, check and records each lichee fresh fruit is corresponding Oviposition paper on Conopomorpha sinensis Bradley ovum number, it is each processing be equipped with 3 repetitions.
Non-selective test: separately the lichee base of a fruit of the 5d or so in spawning period is eaten into after each dependent insect cage accesses 10 pairs of emergence Worm adult (♀: ♂=1:1), by four kinds of medicaments, (treated that lichee fresh fruit is respectively put into Bu Tong supports worm for A, B, C and D) and clear water In cage (10 fruit/cages), and it is put into 10% hydromel of mass fraction, stimulation Conopomorpha sinensis Bradley is laid eggs, and it is fresh to remove lichee afterwards for 24 hours Fruit checks and records the Conopomorpha sinensis Bradley ovum number on the corresponding oviposition paper of each lichee fresh fruit, and each processing is equipped with 3 repetitions.
Four kinds of medicaments (A, B, C and D) are evaluated to the lichee base of a fruit using oviposition detterent rate (Effective Deterrence, ED) The interference effect (formula 1) of moth oviposition behavior.The results are shown in Table 1, and medicament A walks quickly and keeps away rate to Conopomorpha sinensis Bradley oviposition and selecting Property and non-selective test in respectively be up to 73.3 and 53.5, be above other three kinds of chemicals treatment groups, have interfere significantly with litchi The effect of branch base of a fruit moth oviposition behavior.On this basis, initial option medicament A interferes medicine as the Conopomorpha sinensis Bradley Adult worms producting eggs phase Agent.
The different reagent agents of table 1 are to Conopomorpha sinensis Bradley Oviposition Repellent of Alcohol Extracts
Embodiment 2:
Medicament E: 0.01g diflubenzuron and 20g sec-octyl alcohol polyoxyethylene ether are dissolved in 100g water, mix and medicament E is made.
Laboratory test: using medicament E as test group medicament, using clear water as control group medicament, medicament E and clear water are sprayed respectively In Conopomorpha sinensis Bradley ovum paper card, egg hatch rate (formula 2) is counted two days later.The ovicidal effect of medicament E is evaluated by hatching rate, The result shows that: egg hatch rate is 29.7 ± 1.7%, substantially less than control group hatching rate 97.2 ± 2.1% after medicament E processing. Illustrate that medicament E has significant toxic effect to Conopomorpha sinensis Bradley ovum, on this basis, selects medicament E as the Conopomorpha sinensis Bradley ovum phase Green ovicide.
Hatching rate (%)=hatching worm's ovum number/total worm's ovum number × 100 formula 2
Embodiment 3
Medicament A: 20g Wedelia trilobata ethanol extract and 20g alcohol extracts of Lantana camara are added in 100g water, It mixes and obtains medicament A, i.e., contain 20g Wedelia trilobata ethanol extract and 20g alcohol extracts of Lantana camara in every 100g water.
Medicament E: 0.01g diflubenzuron and 20g sec-octyl alcohol polyoxyethylene ether are dissolved in 100ml water, mix and medicament E is made, Contain 0.01g diflubenzuron and 20g sec-octyl alcohol polyoxyethylene ether in i.e. every 100g water.
A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase, comprising: (1) the Conopomorpha sinensis Bradley adult stage, to plant Litchi and longan fruit tree is sprayed in material resource oviposition detterent agent, and the plant source oviposition detterent agent is medicament A;(2) the Conopomorpha sinensis Bradley ovum phase, Fruit tree is sprayed with green ovicide, the green ovicide is medicament E.
It is as follows to carry out orchard verification test for litchi and longan garden Adult occurrence peak at the beginning of -7 months at the beginning of selection 6 months:
Routine administration group: 20 plants of lichee fruit trees of coprocessing, 5L/ plants of amounts of spraying of effective cypermethrin.
Cooperate with prevention and control group: 20 plants of lichee fruit trees of coprocessing, 2L/ plants of amounts of spraying of effective cypermethrin (medication decrement 60%); Meanwhile (1) Conopomorpha sinensis Bradley adult stage, litchi and longan fruit tree is sprayed with plant source oviposition detterent agent, the amount of spraying is 5L/ plants, institute Stating plant source oviposition detterent agent is medicament A;(2) the Conopomorpha sinensis Bradley ovum phase, that is, after spraying medicament A 2 days, with the spray of green ovicide Fruit tree is spilt, the amount of spraying is 5L/ plants, and the green ovicide is medicament E.
Investigation result:
(1) adult amount
After 3 days, routine administration group is counted with adult quantity on the lichee fruit tree after prevention and control group medication is cooperateed with, it is conventional On medication group litchi Conopomorpha sinensis Bradley adult quantity be 1.86/every plant, cooperate with prevention and control group litchi on Conopomorpha sinensis Bradley at Borer population amount is 3.57/every plant, and collaboration prevention and control group is significantly higher than routine administration group.
After 7 days, Conopomorpha sinensis Bradley adult quantity is 3.83/every plant on routine administration group litchi, cooperates with prevention and control group litchi It is 7.53/every plant that branch, which sets upper Conopomorpha sinensis Bradley adult quantity, and collaboration prevention and control group is significantly higher than routine administration group.
After 14 days, Conopomorpha sinensis Bradley adult quantity is 9.07/every plant on routine administration group litchi, cooperates with prevention and control group litchi It is 8.70/every plant that branch, which sets upper Conopomorpha sinensis Bradley adult quantity, routine administration group with to cooperate with prevention and control group to distinguish unobvious.
(2) borer fruit rate and control efficiency
After 3 days, random stripping fruit detection is carried out with the lichee fruit tree after prevention and control group medication is cooperateed with to routine administration group, it is conventional to use Medicine group lichee borer fruit rate is 0.1%, and cooperateing with prevention and control group lichee borer fruit rate is 0.07%, and the two is compared: collaboration prevention and control group eats into fruit Rate is substantially less than routine administration group, and control efficiency is up to 33.33%.
After 7 days, routine administration group lichee borer fruit rate is 0.17%, and cooperateing with prevention and control group lichee borer fruit rate is 0.10%, two Person compares: collaboration prevention and control group borer fruit rate is substantially less than routine administration group, and control efficiency is up to 40%.
After 14 days, routine administration group lichee borer fruit rate is 0.27%, and cooperateing with prevention and control group lichee borer fruit rate is 0.13%, two Person compares: collaboration prevention and control group borer fruit rate is substantially less than routine administration group, and control efficiency is up to 33.33%.
Embodiment 4:
Medicament E: 0.01g diflubenzuron and 20g sec-octyl alcohol polyoxyethylene ether are dissolved in 100ml water, mix and medicament E is made, Contain 0.01g diflubenzuron and 20g sec-octyl alcohol polyoxyethylene ether in i.e. every 100g water.
Medicament F: 10g Wedelia trilobata ethanol extract and 30g alcohol extracts of Lantana camara are added in 100g water, It mixes and obtains medicament F, i.e., contain 10g Wedelia trilobata ethanol extract and 30g alcohol extracts of Lantana camara in every 100g water.
Medicament G: 30g Wedelia trilobata ethanol extract and 10g alcohol extracts of Lantana camara are added in 100g water, It mixes and obtains medicament G, i.e., contain 30g Wedelia trilobata ethanol extract and 10g alcohol extracts of Lantana camara in every 100g water.
A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase, comprising: (1) the Conopomorpha sinensis Bradley adult stage, to plant Litchi and longan fruit tree is sprayed in material resource oviposition detterent agent, and the plant source oviposition detterent agent is medicament F or G;(2) Conopomorpha sinensis Bradley ovum Phase sprays fruit tree with green ovicide, and the green ovicide is medicament E.
It is as follows to carry out orchard verification test for litchi and longan garden Adult occurrence peak at the beginning of -7 months at the beginning of selection 6 months:
Cooperate with prevention and control group: 20 plants of lichee fruit trees of coprocessing, 2L/ plants of amounts of spraying of effective cypermethrin (medication decrement 60%); Meanwhile (1) Conopomorpha sinensis Bradley adult stage, litchi and longan fruit tree is sprayed with plant source oviposition detterent agent, the amount of spraying is 5L/ plants, institute Stating plant source oviposition detterent agent is medicament F or G;(2) the Conopomorpha sinensis Bradley ovum phase, that is, after spraying medicament A2 days, with green ovicide Fruit tree is sprayed, the amount of spraying is 5L/ plants, and the green ovicide is medicament E.
It can be seen that the implementation in Conopomorpha sinensis Bradley adult stage and ovum phase collaboration Control Technology, although in adult quantitative aspects It is unobvious to Conopomorpha sinensis Bradley control, but conventional chemical applications of pesticide amount can be effectively reduced, egg hatch can be substantially reduced Rate and reduction lichee borer fruit rate, reach preferable fruit retention effect.

Claims (10)

1. a kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase, which is characterized in that (1) the Conopomorpha sinensis Bradley adult stage, Litchi and longan fruit tree is sprayed with Conopomorpha sinensis Bradley adult repellant;(2) the Conopomorpha sinensis Bradley ovum phase, with the spray of Conopomorpha sinensis Bradley ovicide Spill fruit tree.
2. the Conopomorpha sinensis Bradley adult stage according to claim 1 cooperates with preventing control method with the ovum phase, which is characterized in that described Conopomorpha sinensis Bradley adult repellant comprising Wedelia trilobata ethanol extract and alcohol extracts of Lantana camara.
3. the Conopomorpha sinensis Bradley adult stage according to claim 2 cooperates with preventing control method with the ovum phase, which is characterized in that described Conopomorpha sinensis Bradley adult repellant is prepared by the following method, and Wedelia trilobata cauline leaf ethanol extract is added in water With lantana cauline leaf ethanol extract.
4. the Conopomorpha sinensis Bradley adult stage according to claim 2 or 3 cooperates with preventing control method with the ovum phase, which is characterized in that institute The Wedelia trilobata ethanol extract and alcohol extracts of Lantana camara stated are respectively Wedelia trilobata cauline leaf ethanol extract With lantana cauline leaf ethanol extract, the Wedelia trilobata cauline leaf ethanol extract is the Wedelia trilobata that will be dried Cauline leaf uses alcohol steep after crushing, and Wedelia trilobata cauline leaf ethanol extract is obtained after the concentrated drying of ethanol extract;Institute The lantana cauline leaf ethanol extract stated is to use alcohol steep after the lantana cauline leaf that will be dried crushes, and ethanol extract is concentrated Lantana cauline leaf ethanol extract is obtained after drying.
5. the Conopomorpha sinensis Bradley adult stage according to claim 4 cooperates with preventing control method with the ovum phase, which is characterized in that described Conopomorpha sinensis Bradley adult repellant is prepared by the following method, and 10-30g Wedelia trilobata stem is added in every 100g water Leaf ethanol extract and 10-30g lantana cauline leaf ethanol extract, thus obtain Conopomorpha sinensis Bradley adult repellant.
6. the Conopomorpha sinensis Bradley adult stage according to claim 1 cooperates with preventing control method with the ovum phase, which is characterized in that described Conopomorpha sinensis Bradley ovicide includes diflubenzuron and sec-octyl alcohol polyoxyethylene ether.
7. the Conopomorpha sinensis Bradley adult stage according to claim 6 cooperates with preventing control method with the ovum phase, which is characterized in that described Conopomorpha sinensis Bradley ovicide is that 0.01g diflubenzuron and 20g sec-octyl alcohol polyoxyethylene ether is added in every 100g water and obtains lichee Base of a fruit moth ovicide.
8. the Conopomorpha sinensis Bradley adult stage according to claim 1 cooperates with preventing control method with the ovum phase, which is characterized in that it is implemented Frequency are as follows: from lichee fruiting early period to receiving fruiting period, every month implements 1 time.
9. according to claim 1, the Conopomorpha sinensis Bradley adult stage described in 5 or 7 cooperates with preventing control method with the ovum phase, which is characterized in that Conopomorpha sinensis Bradley adult repellant sprinkling position is tender tip and the fruit position of litchi and longan fruit tree, and the amount of spraying is 5L/ plants.
10. according to claim 1, the Conopomorpha sinensis Bradley adult stage described in 5 or 7 cooperates with preventing control method with the ovum phase, which is characterized in that It is the amount of spraying after Conopomorpha sinensis Bradley adult repellant sprays work completion 2 days that the Conopomorpha sinensis Bradley ovicide spraying time, which is selected, It is 5L/ plants.
CN201910093533.1A 2019-01-30 2019-01-30 Litchi fruit borer adult stage and egg stage cooperative prevention and control method Active CN109874810B (en)

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